AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032181

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032181.t1 MTR_2g099030 99.471 378 2 0 1 378 30 407 0.0 784
MS.gene032181.t1 MTR_2g087780 46.825 378 189 7 1 374 23 392 3.25e-110 328
MS.gene032181.t1 MTR_7g490350 49.408 338 160 5 37 372 36 364 2.83e-106 317
MS.gene032181.t1 MTR_7g090300 48.910 321 154 4 53 372 53 364 6.68e-104 311
MS.gene032181.t1 MTR_7g490330 48.338 331 123 4 43 372 37 320 1.37e-101 303
MS.gene032181.t1 MTR_4g050190 43.351 376 204 5 1 374 20 388 1.83e-101 305
MS.gene032181.t1 MTR_5g091980 45.483 321 124 5 53 372 48 318 8.45e-85 261
MS.gene032181.t1 MTR_7g090320 38.056 360 202 6 21 367 26 377 2.49e-83 259
MS.gene032181.t1 MTR_7g090310 39.222 334 181 7 47 367 52 376 2.62e-82 256
MS.gene032181.t1 MTR_4g112670 33.533 334 193 4 41 373 27 332 9.97e-62 202
MS.gene032181.t1 MTR_4g112650 33.136 338 193 4 41 375 26 333 2.95e-59 195
MS.gene032181.t1 MTR_4g112630 33.731 335 178 5 41 371 26 320 1.48e-58 193
MS.gene032181.t1 MTR_2g058450 33.531 337 192 6 41 375 28 334 4.27e-55 184
MS.gene032181.t1 MTR_4g112620 29.518 332 167 5 41 371 30 295 7.30e-43 151
MS.gene032181.t1 MTR_8g074270 32.911 316 165 9 33 347 60 329 4.46e-42 151
MS.gene032181.t1 MTR_8g074170 34.317 271 148 6 33 303 60 300 4.08e-41 149
MS.gene032181.t1 MTR_8g074210 33.721 258 143 6 48 305 73 302 3.87e-39 143
MS.gene032181.t1 MTR_2g042370 29.329 283 189 6 95 376 84 356 4.70e-36 135
MS.gene032181.t1 MTR_2g058410 32.031 256 147 4 122 375 1 231 6.07e-34 126
MS.gene032181.t1 MTR_2g042430 26.899 316 209 6 64 378 75 369 3.36e-33 127
MS.gene032181.t1 MTR_2g042420 27.445 317 202 7 64 376 56 348 1.53e-31 122
MS.gene032181.t1 MTR_0003s0530 29.268 205 121 3 165 368 1 182 4.26e-25 101
MS.gene032181.t1 MTR_1g075865 41.905 105 61 0 170 274 7 111 1.27e-24 98.2
MS.gene032181.t1 MTR_8g074320 32.544 169 104 6 78 242 58 220 9.57e-20 87.4
MS.gene032181.t1 MTR_4g112840 38.776 98 60 0 198 295 63 160 1.19e-18 82.8
MS.gene032181.t1 MTR_2g058430 35.789 95 58 1 115 206 54 148 1.23e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032181.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene032181.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene032181.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene032181.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene032181.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene032181.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene032181.t1 AT3G57030 49.371 318 149 5 53 367 56 364 9.88e-105 313
MS.gene032181.t1 AT1G08470 41.995 381 209 7 1 378 19 390 1.55e-100 303
MS.gene032181.t1 AT3G57020 41.250 320 174 5 51 367 51 359 4.35e-81 253
MS.gene032181.t1 AT3G57010 42.105 323 167 7 51 367 52 360 3.07e-78 246
MS.gene032181.t1 AT2G41300 41.667 312 167 5 59 367 84 383 1.91e-76 241
MS.gene032181.t1 AT3G57020 39.375 320 166 5 51 367 51 345 2.30e-75 238
MS.gene032181.t1 AT2G41290 39.939 328 176 8 51 367 49 366 2.06e-71 228
MS.gene032181.t1 AT1G74020 32.915 319 171 9 53 367 38 317 8.34e-50 171
MS.gene032181.t1 AT1G74010 33.129 326 178 9 44 367 28 315 8.05e-47 162
MS.gene032181.t1 AT1G74000 33.438 320 168 11 53 367 40 319 5.39e-46 160
MS.gene032181.t1 AT3G51440 30.746 335 183 9 54 378 70 365 6.45e-33 127
MS.gene032181.t1 AT3G51430 28.657 335 190 12 54 378 70 365 2.60e-31 122
MS.gene032181.t1 AT3G51430 28.060 335 190 12 54 378 70 363 5.76e-31 121
MS.gene032181.t1 AT3G51450 29.080 337 186 12 54 378 70 365 3.63e-30 119
MS.gene032181.t1 AT3G51420 26.727 333 190 12 54 375 70 359 4.15e-30 119

Find 95 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTCGAAAATGAAGTATT+TGG 0.199215 2.3:-4488665 MS.gene032181:CDS
TTCTTCATGACTTGTTTATA+TGG 0.232247 2.3:+4488734 None:intergenic
CATTCCACTGTTCCATCTTT+AGG 0.248214 2.3:+4487223 None:intergenic
GCCAACAAGCTCATTCTTAC+TGG 0.269587 2.3:+4487058 None:intergenic
TGCATATTATGGTCTTCTTA+TGG 0.279511 2.3:-4488076 MS.gene032181:CDS
TTCTCATGTTTCCATTGCTT+TGG 0.287013 2.3:+4488162 None:intergenic
CAAGACAACATGAGTTGTTT+TGG 0.309300 2.3:+4487615 None:intergenic
TACATAGCAGATGCATATTA+TGG 0.318723 2.3:-4488087 MS.gene032181:CDS
AATTGGTGGTTTCGGTAAAA+AGG 0.329225 2.3:+4487539 None:intergenic
TCATTCATTCTTACGTTGTC+TGG 0.331202 2.3:+4487178 None:intergenic
AAACCTGCAATTGGTGGTTT+CGG 0.351201 2.3:+4487531 None:intergenic
ATAAACAAGTCATGAAGAAT+TGG 0.371511 2.3:-4488730 MS.gene032181:CDS
AAGAGGAGAAAGGAAAGCTT+TGG 0.372961 2.3:-4486919 MS.gene032181:CDS
TTCGAGATTTGCACTCCATT+AGG 0.378181 2.3:+4487577 None:intergenic
AACGTAAGAATGAATGAAAA+AGG 0.383654 2.3:-4487172 MS.gene032181:CDS
CTTCTTATGGTTGGACCAAA+TGG 0.385238 2.3:-4488063 MS.gene032181:CDS
AACACGACCATCGGCTAAAC+CGG 0.389147 2.3:+4488598 None:intergenic
AAGATCATTTGCAAAGAGAA+TGG 0.401336 2.3:+4487999 None:intergenic
TGGTAGGAGGATCATATCTA+AGG 0.402934 2.3:+4487635 None:intergenic
CCTAGTGGACCTAAATGAAA+AGG 0.405868 2.3:+4488794 None:intergenic
ATGACAGGCTAATGAAACTT+TGG 0.408963 2.3:-4487255 MS.gene032181:intron
TATTATGGTCTTCTTATGGT+TGG 0.409388 2.3:-4488072 MS.gene032181:CDS
TTACCGAAACCACCAATTGC+AGG 0.427515 2.3:-4487534 MS.gene032181:intron
ATCTTGATATTCACAAGAAC+GGG 0.427957 2.3:-4487981 MS.gene032181:CDS
CTAATGAAACTTTGGATAGA+AGG 0.434236 2.3:-4487247 MS.gene032181:CDS
CCATCGGCTAAACCGGTGTA+AGG 0.444345 2.3:+4488605 None:intergenic
GCAATAGATTGTTGTCGAAC+TGG 0.446574 2.3:-4487136 MS.gene032181:CDS
CTTACGTTGTCTGGAAATCC+TGG 0.449914 2.3:+4487187 None:intergenic
TGGTCGTGTTGTCCGATGGA+TGG 0.450319 2.3:-4488585 MS.gene032181:CDS
GGTGTAGCCAGTCCTCCATT+TGG 0.451029 2.3:+4488048 None:intergenic
GCCAGTAAGAATGAGCTTGT+TGG 0.451834 2.3:-4487059 MS.gene032181:CDS
AGATCATTTGCAAAGAGAAT+GGG 0.452460 2.3:+4488000 None:intergenic
GGTCGTGTTGTCCGATGGAT+GGG 0.455520 2.3:-4488584 MS.gene032181:CDS
CAAAACAACTCATGTTGTCT+TGG 0.456064 2.3:-4487614 MS.gene032181:CDS
TTAAGATTTGACAAAGAGAG+TGG 0.459619 2.3:-4488117 MS.gene032181:CDS
CCTTCAACATGGGTTGCCAA+TGG 0.466610 2.3:+4488027 None:intergenic
GCTGGTTAGTGAAGTTAAAG+AGG 0.470251 2.3:-4486936 MS.gene032181:CDS
TTGCAGTTGTCACATCCAAT+TGG 0.472198 2.3:-4488538 MS.gene032181:intron
GAATGCGTAGCTGACCTTCC+AGG 0.474792 2.3:-4487205 MS.gene032181:CDS
GAGCTTGTTGGCTAAAGCTA+TGG 0.475927 2.3:-4487047 MS.gene032181:CDS
ATTGCACTTCTTGATGATAA+CGG 0.476011 2.3:-4487004 MS.gene032181:CDS
ATGAGCAGACTCGGACTTCA+CGG 0.478710 2.3:-4488695 MS.gene032181:CDS
GAGAAAGGAAAGCTTTGGAT+TGG 0.479363 2.3:-4486914 MS.gene032181:CDS
TTTATATTGTTGGAAGGAGA+AGG 0.479834 2.3:-4487670 MS.gene032181:CDS
CTTTGTCAAATCTTAAACCA+AGG 0.491441 2.3:+4488124 None:intergenic
GTCACTTGAGTTCGATAACA+TGG 0.494746 2.3:-4488636 MS.gene032181:CDS
AGCTTGTTGGCTAAAGCTAT+GGG 0.498617 2.3:-4487046 MS.gene032181:CDS
CGAAAATGAAGTATTTGGAC+CGG 0.503096 2.3:-4488660 MS.gene032181:CDS
AAAACATGTGGGCGTCCCCT+TGG 0.510216 2.3:-4488141 MS.gene032181:CDS
AGTCTGCTCATGTTGTCCTT+AGG 0.510827 2.3:+4488707 None:intergenic
TGGATAGAAGGTCCTAAAGA+TGG 0.515483 2.3:-4487235 MS.gene032181:CDS
GTCGTGTTGTCCGATGGATG+GGG 0.520866 2.3:-4488583 MS.gene032181:CDS
AGTGAAGTTAAAGAGGAGAA+AGG 0.523089 2.3:-4486929 MS.gene032181:CDS
CCATTGGCAACCCATGTTGA+AGG 0.526859 2.3:-4488027 MS.gene032181:CDS
TGGAGGACTGGCTACACCAT+TGG 0.527000 2.3:-4488043 MS.gene032181:CDS
ACAACTCATGTTGTCTTGGA+TGG 0.528253 2.3:-4487610 MS.gene032181:CDS
CTAGTGGACCTAAATGAAAA+GGG 0.529354 2.3:+4488795 None:intergenic
TTGTTACTCAAGACTTCTTG+AGG 0.540862 2.3:+4487112 None:intergenic
TTTGTCAAATCTTAAACCAA+GGG 0.542290 2.3:+4488125 None:intergenic
TTGTTGGAAGGAGAAGGAAC+AGG 0.544523 2.3:-4487664 MS.gene032181:CDS
CCTTACACCGGTTTAGCCGA+TGG 0.545860 2.3:-4488605 MS.gene032181:CDS
GGTGCAACGTTGTGTTTCAC+AGG 0.546534 2.3:+4488755 None:intergenic
CCGATGGTCGTGTTGTCCGA+TGG 0.555843 2.3:-4488589 MS.gene032181:CDS
AAGTCTTGAGTAACAATCCA+TGG 0.565030 2.3:-4487105 MS.gene032181:CDS
GGTTGGACCAAATGGAGGAC+TGG 0.566119 2.3:-4488055 MS.gene032181:CDS
GAGTTCGATAACATGGGCCG+TGG 0.566184 2.3:-4488629 MS.gene032181:CDS
AAGGACAACATGAGCAGACT+CGG 0.574652 2.3:-4488704 MS.gene032181:CDS
GATCTTGATATTCACAAGAA+CGG 0.577423 2.3:-4487982 MS.gene032181:CDS
GACTGAGAAGATTTGCATGA+GGG 0.580450 2.3:-4488202 MS.gene032181:CDS
ATGGGCCGTGGCCCTTACAC+CGG 0.582691 2.3:-4488617 MS.gene032181:CDS
ACTCTACAACTCCAAAGCAA+TGG 0.585049 2.3:-4488173 MS.gene032181:CDS
CCATCGGACAACACGACCAT+CGG 0.587160 2.3:+4488589 None:intergenic
CTAAATTCATGTTCTCCTAG+TGG 0.592914 2.3:+4488779 None:intergenic
CTTATGGTTGGACCAAATGG+AGG 0.594581 2.3:-4488060 MS.gene032181:CDS
AGAAGGCAAAGTGATGAAGC+TGG 0.599296 2.3:-4486954 MS.gene032181:CDS
AGTCATGAAGAATTGGCCTA+AGG 0.608763 2.3:-4488723 MS.gene032181:CDS
GGACTGAGAAGATTTGCATG+AGG 0.612890 2.3:-4488203 MS.gene032181:CDS
CTAAACCGGTGTAAGGGCCA+CGG 0.618440 2.3:+4488612 None:intergenic
TTATCGAACTCAAGTGACTC+CGG 0.620156 2.3:+4488641 None:intergenic
TGGAAACATGAGAAAACATG+TGG 0.620861 2.3:-4488153 MS.gene032181:CDS
TGAGTAACAATCCATGGTTG+AGG 0.621036 2.3:-4487099 MS.gene032181:CDS
ACTGAGAAGATTTGCATGAG+GGG 0.622020 2.3:-4488201 MS.gene032181:CDS
GCTTGTGTCAGTGAAGAAGA+TGG 0.622137 2.3:+4487957 None:intergenic
AGCTCATTCTTACTGGCAAG+CGG 0.627455 2.3:+4487065 None:intergenic
GAAGTTCTTGAAGATCGAGA+AGG 0.628335 2.3:-4486971 MS.gene032181:CDS
CATCGGCTAAACCGGTGTAA+GGG 0.633079 2.3:+4488606 None:intergenic
CGTTGTCTGGAAATCCTGGA+AGG 0.633711 2.3:+4487191 None:intergenic
TCGTGTTGTCCGATGGATGG+GGG 0.655214 2.3:-4488582 MS.gene032181:CDS
TTGTCAAATCTTAAACCAAG+GGG 0.656805 2.3:+4488126 None:intergenic
AAGTAAATACTCCTCAACCA+TGG 0.660467 2.3:+4487088 None:intergenic
TCACTTGAGTTCGATAACAT+GGG 0.668236 2.3:-4488635 MS.gene032181:CDS
AGGTCCTAAAGATGGAACAG+TGG 0.678364 2.3:-4487227 MS.gene032181:CDS
TGGAAGGAGAAGGAACAGGT+AGG 0.689818 2.3:-4487660 MS.gene032181:CDS
GGAAACATGAGAAAACATGT+GGG 0.709710 2.3:-4488152 MS.gene032181:CDS
GCTTGTTGGCTAAAGCTATG+GGG 0.726642 2.3:-4487045 MS.gene032181:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTAATATAAAATA+CGG + chr2.3:4487842-4487861 None:intergenic 0.0%
!! AAAAAATCTACAATAAAATT+TGG - chr2.3:4487855-4487874 MS.gene032181:intron 10.0%
!!! ACATAATATTTTAGACTTAA+TGG + chr2.3:4487396-4487415 None:intergenic 15.0%
!!! TAATGAATTAGTTATTTTGT+TGG + chr2.3:4488387-4488406 None:intergenic 15.0%
!! AATCTACAATAAAATTTGGA+CGG - chr2.3:4487859-4487878 MS.gene032181:intron 20.0%
!! CTTAGCTGAAATAAATAAAT+AGG + chr2.3:4487943-4487962 None:intergenic 20.0%
!!! TGAAACTTGGTATTTTTAAA+AGG - chr2.3:4487447-4487466 MS.gene032181:intron 20.0%
! AAACAATAACACTCATCATA+AGG + chr2.3:4487967-4487986 None:intergenic 25.0%
! AAATAAATAAATAGGATCCC+TGG + chr2.3:4487935-4487954 None:intergenic 25.0%
! AACGTAAGAATGAATGAAAA+AGG - chr2.3:4488499-4488518 MS.gene032181:intron 25.0%
! ATAAACAAGTCATGAAGAAT+TGG - chr2.3:4486941-4486960 MS.gene032181:CDS 25.0%
! ATCATTTGATCTCAATTCAA+CGG + chr2.3:4487805-4487824 None:intergenic 25.0%
! GAGAAAATGCAAAATAAGTA+CGG + chr2.3:4488198-4488217 None:intergenic 25.0%
! TCATTACATTCTTTAATGAC+AGG - chr2.3:4488401-4488420 MS.gene032181:intron 25.0%
! TGTAATACTTGAGGTTAAAT+GGG - chr2.3:4488225-4488244 MS.gene032181:intron 25.0%
! TTGTAATACTTGAGGTTAAA+TGG - chr2.3:4488224-4488243 MS.gene032181:intron 25.0%
! TTTGTCAAATCTTAAACCAA+GGG + chr2.3:4487549-4487568 None:intergenic 25.0%
!! GAATTCGAAAATGAAGTATT+TGG - chr2.3:4487006-4487025 MS.gene032181:CDS 25.0%
!! TACATTGTGATCTTTTATCT+TGG - chr2.3:4488266-4488285 MS.gene032181:intron 25.0%
!!! CACTTTTTTATATTGTTGGA+AGG - chr2.3:4487995-4488014 MS.gene032181:CDS 25.0%
!!! CGCTAAGGTTTTTTAAAAAA+TGG + chr2.3:4488301-4488320 None:intergenic 25.0%
!!! TGCACACTTTTTTATATTGT+TGG - chr2.3:4487991-4488010 MS.gene032181:CDS 25.0%
!!! TTCTTCATGACTTGTTTATA+TGG + chr2.3:4486940-4486959 None:intergenic 25.0%
AAGATCATTTGCAAAGAGAA+TGG + chr2.3:4487675-4487694 None:intergenic 30.0%
AGATCATTTGCAAAGAGAAT+GGG + chr2.3:4487674-4487693 None:intergenic 30.0%
ATCTTGATATTCACAAGAAC+GGG - chr2.3:4487690-4487709 MS.gene032181:intron 30.0%
CTAATGAAACTTTGGATAGA+AGG - chr2.3:4488424-4488443 MS.gene032181:intron 30.0%
CTTTGTCAAATCTTAAACCA+AGG + chr2.3:4487550-4487569 None:intergenic 30.0%
GATCTTGATATTCACAAGAA+CGG - chr2.3:4487689-4487708 MS.gene032181:intron 30.0%
TACATAGCAGATGCATATTA+TGG - chr2.3:4487584-4487603 MS.gene032181:CDS 30.0%
TATTATGGTCTTCTTATGGT+TGG - chr2.3:4487599-4487618 MS.gene032181:CDS 30.0%
TGCATATTATGGTCTTCTTA+TGG - chr2.3:4487595-4487614 MS.gene032181:CDS 30.0%
TTAAGATTTGACAAAGAGAG+TGG - chr2.3:4487554-4487573 MS.gene032181:CDS 30.0%
TTGTCAAATCTTAAACCAAG+GGG + chr2.3:4487548-4487567 None:intergenic 30.0%
TTTATATTGTTGGAAGGAGA+AGG - chr2.3:4488001-4488020 MS.gene032181:CDS 30.0%
! ATTGCACTTCTTGATGATAA+CGG - chr2.3:4488667-4488686 MS.gene032181:CDS 30.0%
! GAATGAAAAAGGACAGTTTT+GGG - chr2.3:4488510-4488529 MS.gene032181:intron 30.0%
! TGAATGAAAAAGGACAGTTT+TGG - chr2.3:4488509-4488528 MS.gene032181:intron 30.0%
!! GTTATTTTGTTGGACGAATT+TGG + chr2.3:4488377-4488396 None:intergenic 30.0%
AAGTAAATACTCCTCAACCA+TGG + chr2.3:4488586-4488605 None:intergenic 35.0%
AAGTCTTGAGTAACAATCCA+TGG - chr2.3:4488566-4488585 MS.gene032181:CDS 35.0%
AGTGAAGTTAAAGAGGAGAA+AGG - chr2.3:4488742-4488761 MS.gene032181:CDS 35.0%
AGTTAAAAACCTGCAATTGG+TGG + chr2.3:4488149-4488168 None:intergenic 35.0%
ATAAATAGGATCCCTGGATT+CGG + chr2.3:4487929-4487948 None:intergenic 35.0%
ATGACAGGCTAATGAAACTT+TGG - chr2.3:4488416-4488435 MS.gene032181:intron 35.0%
ATTGCAATGTGTCTCTCTAT+AGG + chr2.3:4487267-4487286 None:intergenic 35.0%
CAAAACAACTCATGTTGTCT+TGG - chr2.3:4488057-4488076 MS.gene032181:CDS 35.0%
CATTAGCATGAATGCACATA+TGG + chr2.3:4487429-4487448 None:intergenic 35.0%
CTAAATTCATGTTCTCCTAG+TGG + chr2.3:4486895-4486914 None:intergenic 35.0%
GCATTCATGCTAATGAAACT+TGG - chr2.3:4487434-4487453 MS.gene032181:intron 35.0%
GGAAACATGAGAAAACATGT+GGG - chr2.3:4487519-4487538 MS.gene032181:intron 35.0%
TCACTTGAGTTCGATAACAT+GGG - chr2.3:4487036-4487055 MS.gene032181:CDS 35.0%
TCATTCATTCTTACGTTGTC+TGG + chr2.3:4488496-4488515 None:intergenic 35.0%
TCTGCAACTTGTAATACTTG+AGG - chr2.3:4488216-4488235 MS.gene032181:intron 35.0%
TGCAGTTAAAAACCTGCAAT+TGG + chr2.3:4488152-4488171 None:intergenic 35.0%
TGGAAACATGAGAAAACATG+TGG - chr2.3:4487518-4487537 MS.gene032181:intron 35.0%
TTCTCATGTTTCCATTGCTT+TGG + chr2.3:4487512-4487531 None:intergenic 35.0%
TTGTTACTCAAGACTTCTTG+AGG + chr2.3:4488562-4488581 None:intergenic 35.0%
! AATTGGTGGTTTCGGTAAAA+AGG + chr2.3:4488135-4488154 None:intergenic 35.0%
! CAAGACAACATGAGTTGTTT+TGG + chr2.3:4488059-4488078 None:intergenic 35.0%
! CGAAAATGAAGTATTTGGAC+CGG - chr2.3:4487011-4487030 MS.gene032181:CDS 35.0%
! CTAGTGGACCTAAATGAAAA+GGG + chr2.3:4486879-4486898 None:intergenic 35.0%
! TCTTGATGATACTCACCAAT+TGG + chr2.3:4487151-4487170 None:intergenic 35.0%
!! AATGGGTTTTCCTTCAACAT+GGG + chr2.3:4487657-4487676 None:intergenic 35.0%
!!! ACAACATGAGTTGTTTTGGT+AGG + chr2.3:4488055-4488074 None:intergenic 35.0%
!!! GATGGTCTTGTTTTTCCTAA+TGG - chr2.3:4488079-4488098 MS.gene032181:CDS 35.0%
AAACCTTAGCGACACGAAAT+TGG - chr2.3:4488310-4488329 MS.gene032181:intron 40.0%
AAGAGGAGAAAGGAAAGCTT+TGG - chr2.3:4488752-4488771 MS.gene032181:CDS 40.0%
ACAACTCATGTTGTCTTGGA+TGG - chr2.3:4488061-4488080 MS.gene032181:CDS 40.0%
ACTCTACAACTCCAAAGCAA+TGG - chr2.3:4487498-4487517 MS.gene032181:intron 40.0%
ACTGAGAAGATTTGCATGAG+GGG - chr2.3:4487470-4487489 MS.gene032181:intron 40.0%
CATTCCACTGTTCCATCTTT+AGG + chr2.3:4488451-4488470 None:intergenic 40.0%
CTTCTTATGGTTGGACCAAA+TGG - chr2.3:4487608-4487627 MS.gene032181:CDS 40.0%
GAAGTTCTTGAAGATCGAGA+AGG - chr2.3:4488700-4488719 MS.gene032181:CDS 40.0%
GACTGAGAAGATTTGCATGA+GGG - chr2.3:4487469-4487488 MS.gene032181:intron 40.0%
GCAATAGATTGTTGTCGAAC+TGG - chr2.3:4488535-4488554 MS.gene032181:intron 40.0%
GCTGCAGTATGATGTTGAAA+CGG + chr2.3:4488344-4488363 None:intergenic 40.0%
GCTGGTTAGTGAAGTTAAAG+AGG - chr2.3:4488735-4488754 MS.gene032181:CDS 40.0%
GTCACTTGAGTTCGATAACA+TGG - chr2.3:4487035-4487054 MS.gene032181:CDS 40.0%
TAAATAGGATCCCTGGATTC+GGG + chr2.3:4487928-4487947 None:intergenic 40.0%
TGAGTAACAATCCATGGTTG+AGG - chr2.3:4488572-4488591 MS.gene032181:CDS 40.0%
TGGATAGAAGGTCCTAAAGA+TGG - chr2.3:4488436-4488455 MS.gene032181:intron 40.0%
TGGTAGGAGGATCATATCTA+AGG + chr2.3:4488039-4488058 None:intergenic 40.0%
TTATCGAACTCAAGTGACTC+CGG + chr2.3:4487033-4487052 None:intergenic 40.0%
TTCGAGATTTGCACTCCATT+AGG + chr2.3:4488097-4488116 None:intergenic 40.0%
TTGCAGTTGTCACATCCAAT+TGG - chr2.3:4487133-4487152 MS.gene032181:CDS 40.0%
! AAACCTGCAATTGGTGGTTT+CGG + chr2.3:4488143-4488162 None:intergenic 40.0%
! AGTCATGAAGAATTGGCCTA+AGG - chr2.3:4486948-4486967 MS.gene032181:CDS 40.0%
! CCTAGTGGACCTAAATGAAA+AGG + chr2.3:4486880-4486899 None:intergenic 40.0%
! CCTTTTCATTTAGGTCCACT+AGG - chr2.3:4486877-4486896 MS.gene032181:CDS 40.0%
!! AGCTTGTTGGCTAAAGCTAT+GGG - chr2.3:4488625-4488644 MS.gene032181:CDS 40.0%
!! GAATGGGTTTTCCTTCAACA+TGG + chr2.3:4487658-4487677 None:intergenic 40.0%
!! GAGAAAGGAAAGCTTTGGAT+TGG - chr2.3:4488757-4488776 MS.gene032181:CDS 40.0%
!!! ACATGAGTTGTTTTGGTAGG+AGG + chr2.3:4488052-4488071 None:intergenic 40.0%
!!! TGAAAAAGGACAGTTTTGGG+TGG - chr2.3:4488513-4488532 MS.gene032181:intron 40.0%
AAATAGGATCCCTGGATTCG+GGG + chr2.3:4487927-4487946 None:intergenic 45.0%
AAGGACAACATGAGCAGACT+CGG - chr2.3:4486967-4486986 MS.gene032181:CDS 45.0%
AGAAGGCAAAGTGATGAAGC+TGG - chr2.3:4488717-4488736 MS.gene032181:CDS 45.0%
AGCTCATTCTTACTGGCAAG+CGG + chr2.3:4488609-4488628 None:intergenic 45.0%
AGGTCCTAAAGATGGAACAG+TGG - chr2.3:4488444-4488463 MS.gene032181:intron 45.0%
AGTCTGCTCATGTTGTCCTT+AGG + chr2.3:4486967-4486986 None:intergenic 45.0%
CTTACGTTGTCTGGAAATCC+TGG + chr2.3:4488487-4488506 None:intergenic 45.0%
CTTATGGTTGGACCAAATGG+AGG - chr2.3:4487611-4487630 MS.gene032181:CDS 45.0%
GCCAACAAGCTCATTCTTAC+TGG + chr2.3:4488616-4488635 None:intergenic 45.0%
GCCAGTAAGAATGAGCTTGT+TGG - chr2.3:4488612-4488631 MS.gene032181:CDS 45.0%
GCTTGTGTCAGTGAAGAAGA+TGG + chr2.3:4487717-4487736 None:intergenic 45.0%
GGACGGTCATCTCAATTCAA+CGG - chr2.3:4487876-4487895 MS.gene032181:intron 45.0%
GGACTGAGAAGATTTGCATG+AGG - chr2.3:4487468-4487487 MS.gene032181:intron 45.0%
GGCTATCAACAATCTGACTC+TGG + chr2.3:4487784-4487803 None:intergenic 45.0%
GGTTTCGGTAAAAAGGAGGA+AGG + chr2.3:4488128-4488147 None:intergenic 45.0%
TGGTGGTTTCGGTAAAAAGG+AGG + chr2.3:4488132-4488151 None:intergenic 45.0%
TTACCGAAACCACCAATTGC+AGG - chr2.3:4488137-4488156 MS.gene032181:CDS 45.0%
TTGTTGGAAGGAGAAGGAAC+AGG - chr2.3:4488007-4488026 MS.gene032181:CDS 45.0%
! AGTCCAATTTCGTGTCGCTA+AGG + chr2.3:4488316-4488335 None:intergenic 45.0%
! GCTTGTTGGCTAAAGCTATG+GGG - chr2.3:4488626-4488645 MS.gene032181:CDS 45.0%
!! GAGCTTGTTGGCTAAAGCTA+TGG - chr2.3:4488624-4488643 MS.gene032181:CDS 45.0%
ATGAGCAGACTCGGACTTCA+CGG - chr2.3:4486976-4486995 MS.gene032181:CDS 50.0%
CAAGTTGTTCCCCCATCCAT+CGG + chr2.3:4487101-4487120 None:intergenic 50.0%
CCATTGGCAACCCATGTTGA+AGG - chr2.3:4487644-4487663 MS.gene032181:CDS 50.0%
CGTTGTCTGGAAATCCTGGA+AGG + chr2.3:4488483-4488502 None:intergenic 50.0%
GATGGGGGAACAACTTGGTT+GGG - chr2.3:4487104-4487123 MS.gene032181:CDS 50.0%
GGTGCAACGTTGTGTTTCAC+AGG + chr2.3:4486919-4486938 None:intergenic 50.0%
TGGAAGGAGAAGGAACAGGT+AGG - chr2.3:4488011-4488030 MS.gene032181:CDS 50.0%
! AACACGACCATCGGCTAAAC+CGG + chr2.3:4487076-4487095 None:intergenic 50.0%
! ATGCATTGATCCCCGAATCC+AGG - chr2.3:4487914-4487933 MS.gene032181:intron 50.0%
! CATCGGCTAAACCGGTGTAA+GGG + chr2.3:4487068-4487087 None:intergenic 50.0%
! CCTTCAACATGGGTTGCCAA+TGG + chr2.3:4487647-4487666 None:intergenic 50.0%
! TGCATTGATCCCCGAATCCA+GGG - chr2.3:4487915-4487934 MS.gene032181:intron 50.0%
AAAACATGTGGGCGTCCCCT+TGG - chr2.3:4487530-4487549 MS.gene032181:intron 55.0%
CCATCGGACAACACGACCAT+CGG + chr2.3:4487085-4487104 None:intergenic 55.0%
CGATGGATGGGGGAACAACT+TGG - chr2.3:4487099-4487118 MS.gene032181:CDS 55.0%
CTAAACCGGTGTAAGGGCCA+CGG + chr2.3:4487062-4487081 None:intergenic 55.0%
GAATGCGTAGCTGACCTTCC+AGG - chr2.3:4488466-4488485 MS.gene032181:intron 55.0%
GAGTTCGATAACATGGGCCG+TGG - chr2.3:4487042-4487061 MS.gene032181:CDS 55.0%
GGATGGGGGAACAACTTGGT+TGG - chr2.3:4487103-4487122 MS.gene032181:CDS 55.0%
GGTCGTGTTGTCCGATGGAT+GGG - chr2.3:4487087-4487106 MS.gene032181:CDS 55.0%
GGTGTAGCCAGTCCTCCATT+TGG + chr2.3:4487626-4487645 None:intergenic 55.0%
GGTTGGACCAAATGGAGGAC+TGG - chr2.3:4487616-4487635 MS.gene032181:CDS 55.0%
GTCGTGTTGTCCGATGGATG+GGG - chr2.3:4487088-4487107 MS.gene032181:CDS 55.0%
TCGTGTTGTCCGATGGATGG+GGG - chr2.3:4487089-4487108 MS.gene032181:CDS 55.0%
TGGTCGTGTTGTCCGATGGA+TGG - chr2.3:4487086-4487105 MS.gene032181:CDS 55.0%
! CCATCGGCTAAACCGGTGTA+AGG + chr2.3:4487069-4487088 None:intergenic 55.0%
!! CCTTACACCGGTTTAGCCGA+TGG - chr2.3:4487066-4487085 MS.gene032181:CDS 55.0%
!! TGGAGGACTGGCTACACCAT+TGG - chr2.3:4487628-4487647 MS.gene032181:CDS 55.0%
CCGATGGTCGTGTTGTCCGA+TGG - chr2.3:4487082-4487101 MS.gene032181:CDS 60.0%
ATGGGCCGTGGCCCTTACAC+CGG - chr2.3:4487054-4487073 MS.gene032181:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4486871 4488822 4486871 ID=MS.gene032181
chr2.3 mRNA 4486871 4488822 4486871 ID=MS.gene032181.t1;Parent=MS.gene032181
chr2.3 exon 4488539 4488822 4488539 ID=MS.gene032181.t1.exon1;Parent=MS.gene032181.t1
chr2.3 CDS 4488539 4488822 4488539 ID=cds.MS.gene032181.t1;Parent=MS.gene032181.t1
chr2.3 exon 4487940 4488224 4487940 ID=MS.gene032181.t1.exon2;Parent=MS.gene032181.t1
chr2.3 CDS 4487940 4488224 4487940 ID=cds.MS.gene032181.t1;Parent=MS.gene032181.t1
chr2.3 exon 4487535 4487702 4487535 ID=MS.gene032181.t1.exon3;Parent=MS.gene032181.t1
chr2.3 CDS 4487535 4487702 4487535 ID=cds.MS.gene032181.t1;Parent=MS.gene032181.t1
chr2.3 exon 4486871 4487270 4486871 ID=MS.gene032181.t1.exon4;Parent=MS.gene032181.t1
chr2.3 CDS 4486871 4487270 4486871 ID=cds.MS.gene032181.t1;Parent=MS.gene032181.t1
Gene Sequence

>MS.gene032181

ATGGACCCTTTTCATTTAGGTCCACTAGGAGAACATGAATTTAGACCTGTGAAACACAACGTTGCACCATATAAACAAGTCATGAAGAATTGGCCTAAGGACAACATGAGCAGACTCGGACTTCACGGAAAATTAGAATTCGAAAATGAAGTATTTGGACCGGAGTCACTTGAGTTCGATAACATGGGCCGTGGCCCTTACACCGGTTTAGCCGATGGTCGTGTTGTCCGATGGATGGGGGAACAACTTGGTTGGGAAACTTTTGCAGTTGTCACATCCAATTGGTGAGTATCATCAAGAAATTTGTAATCTAATTTGATAAATCAGCTTTACAATACAACTCAAAATATGACATATAATTTTCATTCAACTATTATGATTCATGACTACGTGCCTATAGAGAGACACATTGCAATTATAATTGTTGAATGAAGATCAGACAATTCACATTTTGTGTTCGAAATATGAGATGTCTAAAATATGAAATGAAGATCTTCACGCAATTGCGTGATTGTATACACTCCATTAAGTCTAAAATATTATGTTTTTATATGTCCATATGTGCATTCATGCTAATGAAACTTGGTATTTTTAAAAGGACTGAGAAGATTTGCATGAGGGGAAATGACTCTACAACTCCAAAGCAATGGAAACATGAGAAAACATGTGGGCGTCCCCTTGGTTTAAGATTTGACAAAGAGAGTGGAGATTTATACATAGCAGATGCATATTATGGTCTTCTTATGGTTGGACCAAATGGAGGACTGGCTACACCATTGGCAACCCATGTTGAAGGAAAACCCATTCTCTTTGCAAATGATCTTGATATTCACAAGAACGGGTCCATCTTCTTCACTGACACAAGCACACGATACAATCGAGTGTAATTTCTTCTATTTCTATCTATTCACCAGAGTCAGATTGTTGATAGCCGTTGAATTGAGATCAAATGATATAGATTTAAAGTTCCGTATTTTATATTAAAAAAAATCTACAATAAAATTTGGACGGTCATCTCAATTCAACGGTTATCGATATGTTAAATGCATTGATCCCCGAATCCAGGGATCCTATTTATTTATTTCAGCTAAGTCCTTATGATGAGTGTTATTGTTTGCAGTGCACACTTTTTTATATTGTTGGAAGGAGAAGGAACAGGTAGGCTCCTTAGATATGATCCTCCTACCAAAACAACTCATGTTGTCTTGGATGGTCTTGTTTTTCCTAATGGAGTGCAAATCTCGAAAGATCAATCCTTCCTCCTTTTTACCGAAACCACCAATTGCAGGTTTTTAACTGCATTAATTATTTCTTTATCAACATACCGTACTTATTTTGCATTTTCTCTGCAACTTGTAATACTTGAGGTTAAATGGGATGCAACTCAATTGTAGTTACATTGTGATCTTTTATCTTGGTGCTGCAAACCATTTTTTAAAAAACCTTAGCGACACGAAATTGGACTCTCTACCGTTTCAACATCATACTGCAGCTATAATGAATCCAAATTCGTCCAACAAAATAACTAATTCATTACATTCTTTAATGACAGGCTAATGAAACTTTGGATAGAAGGTCCTAAAGATGGAACAGTGGAATGCGTAGCTGACCTTCCAGGATTTCCAGACAACGTAAGAATGAATGAAAAAGGACAGTTTTGGGTGGCAATAGATTGTTGTCGAACTGGACCTCAAGAAGTCTTGAGTAACAATCCATGGTTGAGGAGTATTTACTTCCGCTTGCCAGTAAGAATGAGCTTGTTGGCTAAAGCTATGGGGATGAAAATGTACACAATGATTGCACTTCTTGATGATAACGGAAAGATTCTTGAAGTTCTTGAAGATCGAGAAGGCAAAGTGATGAAGCTGGTTAGTGAAGTTAAAGAGGAGAAAGGAAAGCTTTGGATTGGAACAGTAGCTCATAATCACATCTCCACCTTACCTTACCCTTAA
Protein sequence

>MS.gene032181.t1

MDPFHLGPLGEHEFRPVKHNVAPYKQVMKNWPKDNMSRLGLHGKLEFENEVFGPESLEFDNMGRGPYTGLADGRVVRWMGEQLGWETFAVVTSNWTEKICMRGNDSTTPKQWKHEKTCGRPLGLRFDKESGDLYIADAYYGLLMVGPNGGLATPLATHVEGKPILFANDLDIHKNGSIFFTDTSTRYNRVAHFFILLEGEGTGRLLRYDPPTKTTHVVLDGLVFPNGVQISKDQSFLLFTETTNCRLMKLWIEGPKDGTVECVADLPGFPDNVRMNEKGQFWVAIDCCRTGPQEVLSNNPWLRSIYFRLPVRMSLLAKAMGMKMYTMIALLDDNGKILEVLEDREGKVMKLVSEVKEEKGKLWIGTVAHNHISTLPYP