AlfalfaGEDB | MS.gene036302

M. sativa cultivar XinJiangDaYe / MS.gene036302

KEGG: K03019
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036302.t1	RHN54834.1	97.1	68	2	0	1	68	1	68	2.50E-31	144.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036302.t1	A0A072UDK1	97.1	68	2	0	1	68	19	86	1.8e-31	144.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene004127	MS.gene036302	PPI
MS.gene036302	MS.gene051008	PPI
MS.gene90078	MS.gene036302	PPI
MS.gene26950	MS.gene036302	PPI
MS.gene54479	MS.gene036302	PPI
MS.gene025949	MS.gene036302	PPI
MS.gene00182	MS.gene036302	PPI
MS.gene65289	MS.gene036302	PPI
MS.gene050602	MS.gene036302	PPI
MS.gene049822	MS.gene036302	PPI
MS.gene036302	MS.gene65249	PPI
MS.gene036302	MS.gene56011	PPI
MS.gene036302	MS.gene08465	PPI
MS.gene027891	MS.gene036302	PPI
MS.gene80957	MS.gene036302	PPI
MS.gene24295	MS.gene036302	PPI
MS.gene058216	MS.gene036302	PPI
MS.gene036302	MS.gene64653	PPI
MS.gene036302	MS.gene050602	PPI
MS.gene036302	MS.gene65289	PPI
MS.gene22836	MS.gene036302	PPI
MS.gene036302	MS.gene81051	PPI
MS.gene049824	MS.gene036302	PPI
MS.gene036302	MS.gene27326	PPI
MS.gene004126	MS.gene036302	PPI
MS.gene024486	MS.gene036302	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036302.t1	MTR_5g030180	97.059	68	2	0	1	68	6	73	4.02e-44	137
MS.gene036302.t1	MTR_5g030180	97.059	68	2	0	1	68	19	86	5.06e-44	137
MS.gene036302.t1	MTR_7g116750	88.406	69	7	1	1	68	6	74	4.71e-38	122

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene036302.t1	AT4G07950	42.373	59	27	1	2	60	7	58	4.50e-12	56.6

Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCGATTGAGCAAATATA+TGG	0.241781	5.2:-19022806	None:intergenic
ATCTCATGATGGGAGATAAT+AGG	0.295531	5.2:-19022977	None:intergenic
AAATTTGTCGTGACTCGAAT+TGG	0.331864	5.2:-19022753	None:intergenic
AGAGAAGACAAATGCTAGTT+AGG	0.359824	5.2:+19022942	MS.gene036302:CDS
AAAGTAGTACCGTCGGCTTT+TGG	0.414427	5.2:-19023013	None:intergenic
TCTCATGATGGGAGATAATA+GGG	0.429313	5.2:-19022976	None:intergenic
TATTCTTGTTGATCTCATGA+TGG	0.460772	5.2:-19022988	None:intergenic
AATTTGTCGTGACTCGAATT+GGG	0.488382	5.2:-19022752	None:intergenic
ATCAGCATGCTCCCGCACAT+AGG	0.496816	5.2:-19022719	None:intergenic
GACAAATGCTAGTTAGGAAA+GGG	0.497928	5.2:+19022948	MS.gene036302:CDS
ATTCTTGTTGATCTCATGAT+GGG	0.514246	5.2:-19022987	None:intergenic
TATTTGCTCAATCGAGAAAG+AGG	0.528655	5.2:+19022811	MS.gene036302:CDS
GTGAGGCAATAAAACCTTGT+GGG	0.529652	5.2:-19022779	None:intergenic
AGACAAATGCTAGTTAGGAA+AGG	0.561312	5.2:+19022947	MS.gene036302:CDS
AGCAAATATATGGACAAGTG+AGG	0.632197	5.2:-19022796	None:intergenic
ACGACAAATTTCATCCCACA+AGG	0.644788	5.2:+19022765	MS.gene036302:CDS
AAGAATAAACCAAAAGCCGA+CGG	0.660260	5.2:+19023004	MS.gene036302:CDS
AGTGAGGCAATAAAACCTTG+TGG	0.661965	5.2:-19022780	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAGAAAACATGATGAAAT+GGG	-	chr5.2:19022903-19022922	None:intergenic	20.0%
!!	TAAAAGAAAACATGATGAAA+TGG	-	chr5.2:19022904-19022923	None:intergenic	20.0%
!!	CATGTTTTCTTTTACTTTAC+AGG	+	chr5.2:19022910-19022929	MS.gene036302:intron	25.0%
	ATTCTTGTTGATCTCATGAT+GGG	-	chr5.2:19022990-19023009	None:intergenic	30.0%
	TATTCTTGTTGATCTCATGA+TGG	-	chr5.2:19022991-19023010	None:intergenic	30.0%
	TTCTCGATTGAGCAAATATA+TGG	-	chr5.2:19022809-19022828	None:intergenic	30.0%
!!	TAACTGGTTGGTAATAGAAA+TGG	-	chr5.2:19022868-19022887	None:intergenic	30.0%
	AAATTTGTCGTGACTCGAAT+TGG	-	chr5.2:19022756-19022775	None:intergenic	35.0%
	AATTTGTCGTGACTCGAATT+GGG	-	chr5.2:19022755-19022774	None:intergenic	35.0%
	AGACAAATGCTAGTTAGGAA+AGG	+	chr5.2:19022947-19022966	MS.gene036302:CDS	35.0%
	AGAGAAGACAAATGCTAGTT+AGG	+	chr5.2:19022942-19022961	MS.gene036302:CDS	35.0%
	AGCAAATATATGGACAAGTG+AGG	-	chr5.2:19022799-19022818	None:intergenic	35.0%
	GACAAATGCTAGTTAGGAAA+GGG	+	chr5.2:19022948-19022967	MS.gene036302:CDS	35.0%
	TATTTGCTCAATCGAGAAAG+AGG	+	chr5.2:19022811-19022830	MS.gene036302:CDS	35.0%
	TTGAATTGAAGGTAACTGGT+TGG	-	chr5.2:19022880-19022899	None:intergenic	35.0%
!	ATCTCATGATGGGAGATAAT+AGG	-	chr5.2:19022980-19022999	None:intergenic	35.0%
!	TCTCATGATGGGAGATAATA+GGG	-	chr5.2:19022979-19022998	None:intergenic	35.0%
!!	GATGAAATGGGTTGAATTGA+AGG	-	chr5.2:19022891-19022910	None:intergenic	35.0%
	ACGACAAATTTCATCCCACA+AGG	+	chr5.2:19022765-19022784	MS.gene036302:CDS	40.0%
	AGTGAGGCAATAAAACCTTG+TGG	-	chr5.2:19022783-19022802	None:intergenic	40.0%
	GTGAGGCAATAAAACCTTGT+GGG	-	chr5.2:19022782-19022801	None:intergenic	40.0%
!	TGGGTTGAATTGAAGGTAAC+TGG	-	chr5.2:19022884-19022903	None:intergenic	40.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	19022722	19023025	19022722	ID=MS.gene036302
chr5.2	mRNA	19022722	19023025	19022722	ID=MS.gene036302.t1;Parent=MS.gene036302
chr5.2	exon	19022722	19022832	19022722	ID=MS.gene036302.t1.exon1;Parent=MS.gene036302.t1
chr5.2	CDS	19022722	19022832	19022722	ID=cds.MS.gene036302.t1;Parent=MS.gene036302.t1
chr5.2	exon	19022932	19023025	19022932	ID=MS.gene036302.t1.exon2;Parent=MS.gene036302.t1
chr5.2	CDS	19022932	19023025	19022932	ID=cds.MS.gene036302.t1;Parent=MS.gene036302.t1

Gene Sequence	>MS.gene036302 ATGTGCGGGAGCATGCTGATGCTTGAACTACCCAATTCGAGTCACGACAAATTTCATCCCACAAGGTTTTATTGCCTCACTTGTCCATATATTTGCTCAATCGAGAAAGAGGTATGTTATGTTATGTACTCTTCTTCTATTTTCCATTTCTATTACCAACCAGTTACCTTCAATTCAACCCATTTCATCATGTTTTCTTTTACTTTACAGGTTAAGATTAAGAGAAGACAAATGCTAGTTAGGAAAGGGATTGACCCTATTATCTCCCATCATGAGATCAACAAGAATAAACCAAAAGCCGACG
Protein sequence	>MS.gene036302.t1 MCGSMLMLELPNSSHDKFHPTRFYCLTCPYICSIEKEVKIKRRQMLVRKGIDPIISHHEINKNKPKAD