AlfalfaGEDB | MS.gene90078

M. sativa cultivar XinJiangDaYe / MS.gene90078

KEGG: K03019
GO: GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0018130 GO:0019438 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90078.t1	XP_013453676.1	97.3	73	2	0	1	73	14	86	6.70E-35	156

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90078.t1	A0A072UDK1	97.3	73	2	0	1	73	14	86	4.8e-35	156.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene90078	MS.gene22836	PPI
MS.gene90078	MS.gene036302	PPI
MS.gene90078	MS.gene004126	PPI
MS.gene90078	MS.gene64653	PPI
MS.gene90078	MS.gene058216	PPI
MS.gene90078	MS.gene65289	PPI
MS.gene90078	MS.gene024486	PPI
MS.gene90078	MS.gene24295	PPI
MS.gene90078	MS.gene80957	PPI
MS.gene90078	MS.gene049822	PPI
MS.gene90078	MS.gene26950	PPI
MS.gene90078	MS.gene051008	PPI
MS.gene90078	MS.gene81051	PPI
MS.gene80957	MS.gene90078	PPI
MS.gene90078	MS.gene025949	PPI
MS.gene90078	MS.gene00182	PPI
MS.gene90078	MS.gene27326	PPI
MS.gene90078	MS.gene54479	PPI
MS.gene90078	MS.gene08465	PPI
MS.gene90078	MS.gene049824	PPI
MS.gene90078	MS.gene027891	PPI
MS.gene90078	MS.gene004127	PPI
MS.gene90078	MS.gene050602	PPI
MS.gene90078	MS.gene65249	PPI
MS.gene90078	MS.gene56011	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90078.t1	MTR_5g030180	97.260	73	2	0	1	73	1	73	2.62e-48	148
MS.gene90078.t1	MTR_5g030180	97.260	73	2	0	1	73	14	86	3.14e-48	149
MS.gene90078.t1	MTR_7g116750	89.189	74	7	1	1	73	1	74	2.89e-42	133

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90078.t1	AT4G07950	46.154	65	28	1	1	65	1	58	1.23e-16	68.2
MS.gene90078.t1	AT1G01210	41.538	65	31	1	1	65	1	58	1.15e-14	63.2
MS.gene90078.t1	AT1G01210	41.538	65	31	1	1	65	1	58	1.15e-14	63.2
MS.gene90078.t1	AT1G01210	41.538	65	31	1	1	65	1	58	1.15e-14	63.2

Find 19 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCGATTGAGCAAATATA+TGG	0.241781	5.2:-73922	None:intergenic
ATCTCATGATGGGAGATAAT+AGG	0.295531	5.2:-74093	None:intergenic
AAATTTGTCGTGACTCGAAT+TGG	0.331864	5.2:-73869	None:intergenic
AGAGAAGACAAATGCTGGTT+AGG	0.408988	5.2:+74058	MS.gene90078:CDS
AAAGTAGTACCGTCGGCTTT+TGG	0.414427	5.2:-74129	None:intergenic
TCTCATGATGGGAGATAATA+GGG	0.429313	5.2:-74092	None:intergenic
TATTCTTGTTGATCTCATGA+TGG	0.460772	5.2:-74104	None:intergenic
AATTTGTCGTGACTCGAATT+GGG	0.488382	5.2:-73868	None:intergenic
GACAAATGCTGGTTAGGAAA+GGG	0.497928	5.2:+74064	MS.gene90078:CDS
ATTCTTGTTGATCTCATGAT+GGG	0.514246	5.2:-74103	None:intergenic
GATTAAGAGAAGACAAATGC+TGG	0.517033	5.2:+74053	MS.gene90078:CDS
TATTTGCTCAATCGAGAAAG+AGG	0.528655	5.2:+73927	MS.gene90078:CDS
GTGAGGCAATAAAACCTTGT+GGG	0.529652	5.2:-73895	None:intergenic
AGACAAATGCTGGTTAGGAA+AGG	0.564511	5.2:+74063	MS.gene90078:CDS
ATCAGCATGCTCCCGCACAT+AGG	0.589669	5.2:-73835	None:intergenic
AGCAAATATATGGACAAGTG+AGG	0.632197	5.2:-73912	None:intergenic
ACGACAAATTTCATCCCACA+AGG	0.644788	5.2:+73881	MS.gene90078:CDS
AAGAATAAACCAAAAGCCGA+CGG	0.660260	5.2:+74120	MS.gene90078:CDS
AGTGAGGCAATAAAACCTTG+TGG	0.661965	5.2:-73896	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAGAAAACATGATGAAAT+GGG	-	chr5.2:74019-74038	None:intergenic	20.0%
!!	TAAAAGAAAACATGATGAAA+TGG	-	chr5.2:74020-74039	None:intergenic	20.0%
!!	CATGTTTTCTTTTACTTTAC+AGG	+	chr5.2:74026-74045	MS.gene90078:intron	25.0%
	ATTCTTGTTGATCTCATGAT+GGG	-	chr5.2:74106-74125	None:intergenic	30.0%
	TATTCTTGTTGATCTCATGA+TGG	-	chr5.2:74107-74126	None:intergenic	30.0%
	TTCTCGATTGAGCAAATATA+TGG	-	chr5.2:73925-73944	None:intergenic	30.0%
!!	TAACTGGTTGGTAATAGAAA+TGG	-	chr5.2:73984-74003	None:intergenic	30.0%
	AAATTTGTCGTGACTCGAAT+TGG	-	chr5.2:73872-73891	None:intergenic	35.0%
	AATTTGTCGTGACTCGAATT+GGG	-	chr5.2:73871-73890	None:intergenic	35.0%
	AGCAAATATATGGACAAGTG+AGG	-	chr5.2:73915-73934	None:intergenic	35.0%
	GATTAAGAGAAGACAAATGC+TGG	+	chr5.2:74053-74072	MS.gene90078:CDS	35.0%
	TATTTGCTCAATCGAGAAAG+AGG	+	chr5.2:73927-73946	MS.gene90078:CDS	35.0%
	TTGAATTGAAGGTAACTGGT+TGG	-	chr5.2:73996-74015	None:intergenic	35.0%
!	ATCTCATGATGGGAGATAAT+AGG	-	chr5.2:74096-74115	None:intergenic	35.0%
!	TCTCATGATGGGAGATAATA+GGG	-	chr5.2:74095-74114	None:intergenic	35.0%
!!	GATGAAATGGGTTGAATTGA+AGG	-	chr5.2:74007-74026	None:intergenic	35.0%
	ACGACAAATTTCATCCCACA+AGG	+	chr5.2:73881-73900	MS.gene90078:CDS	40.0%
	AGTGAGGCAATAAAACCTTG+TGG	-	chr5.2:73899-73918	None:intergenic	40.0%
	GTGAGGCAATAAAACCTTGT+GGG	-	chr5.2:73898-73917	None:intergenic	40.0%
!	AGACAAATGCTGGTTAGGAA+AGG	+	chr5.2:74063-74082	MS.gene90078:CDS	40.0%
!	AGAGAAGACAAATGCTGGTT+AGG	+	chr5.2:74058-74077	MS.gene90078:CDS	40.0%
!	GACAAATGCTGGTTAGGAAA+GGG	+	chr5.2:74064-74083	MS.gene90078:CDS	40.0%
!	TGGGTTGAATTGAAGGTAAC+TGG	-	chr5.2:74000-74019	None:intergenic	40.0%
!!	ATGGAGTTTTGTCCTATGTG+CGG	+	chr5.2:73823-73842	MS.gene90078:CDS	40.0%
!!	TGGAGTTTTGTCCTATGTGC+GGG	+	chr5.2:73824-73843	MS.gene90078:CDS	45.0%
	ATCAGCATGCTCCCGCACAT+AGG	-	chr5.2:73838-73857	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	73823	74141	73823	ID=MS.gene90078
chr5.2	mRNA	73823	74141	73823	ID=MS.gene90078.t1;Parent=MS.gene90078
chr5.2	exon	73823	73948	73823	ID=MS.gene90078.t1.exon1;Parent=MS.gene90078.t1
chr5.2	CDS	73823	73948	73823	ID=cds.MS.gene90078.t1;Parent=MS.gene90078.t1
chr5.2	exon	74048	74141	74048	ID=MS.gene90078.t1.exon2;Parent=MS.gene90078.t1
chr5.2	CDS	74048	74141	74048	ID=cds.MS.gene90078.t1;Parent=MS.gene90078.t1

Gene Sequence	>MS.gene90078 ATGGAGTTTTGTCCTATGTGCGGGAGCATGCTGATGCTTGAACTACCCAATTCGAGTCACGACAAATTTCATCCCACAAGGTTTTATTGCCTCACTTGTCCATATATTTGCTCAATCGAGAAAGAGGTATGTTATGTTATGTACTCTTCTTCTATTTTCCATTTCTATTACCAACCAGTTACCTTCAATTCAACCCATTTCATCATGTTTTCTTTTACTTTACAGGTTAAGATTAAGAGAAGACAAATGCTGGTTAGGAAAGGGATTGACCCTATTATCTCCCATCATGAGATCAACAAGAATAAACCAAAAGCCGACG
Protein sequence	>MS.gene90078.t1 MEFCPMCGSMLMLELPNSSHDKFHPTRFYCLTCPYICSIEKEVKIKRRQMLVRKGIDPIISHHEINKNKPKAD