AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54479


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54479.t1 MTR_7g116750 92.308 117 9 0 1 117 1 117 8.99e-81 232
MS.gene54479.t1 MTR_5g030180 89.655 116 11 1 1 116 14 128 7.81e-75 218
MS.gene54479.t1 MTR_5g030180 89.655 116 11 1 1 116 1 115 1.04e-74 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54479.t1 AT4G07950 43.103 116 55 4 1 116 1 105 2.17e-28 100
MS.gene54479.t1 AT1G01210 42.241 116 56 4 1 116 1 105 2.48e-27 97.4
MS.gene54479.t1 AT1G01210 42.241 116 56 4 1 116 1 105 2.48e-27 97.4
MS.gene54479.t1 AT1G01210 42.241 116 56 4 1 116 1 105 2.48e-27 97.4
MS.gene54479.t1 AT3G25940 29.839 124 63 5 3 117 11 119 9.15e-11 55.1

Find 38 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGAGGATGGCGACAATT+TGG 0.237510 7.4:+92025039 None:intergenic
ATTTCATCATGGGAGATAAT+AGG 0.283528 7.4:+92026245 None:intergenic
GTCCTGATTGGGCAAATATA+TGG 0.298301 7.4:+92026423 None:intergenic
CACTCGTAAAATATTGTTGC+AGG 0.321821 7.4:+92024979 None:intergenic
CTTGAGGATGGCGACAATTT+GGG 0.324696 7.4:+92025040 None:intergenic
GTCCATATATTTGCCCAATC+AGG 0.365575 7.4:-92026425 MS.gene54479:CDS
TTTCATCATGGGAGATAATA+GGG 0.399931 7.4:+92026246 None:intergenic
TAACATACCCCTGTCCTGAT+TGG 0.407408 7.4:+92026411 None:intergenic
AAAGTAGTACCGTCGGCTTT+TGG 0.414427 7.4:+92026209 None:intergenic
AACATACCCCTGTCCTGATT+GGG 0.444565 7.4:+92026412 None:intergenic
TTTATCCCTCCTTCCACTTG+TGG 0.445466 7.4:+92024938 None:intergenic
ATTCTTGTTGATTTCATCAT+GGG 0.448511 7.4:+92026235 None:intergenic
TATTCTTGTTGATTTCATCA+TGG 0.453333 7.4:+92026234 None:intergenic
GTGCCGTGACTCGTAGTATT+GGG 0.460519 7.4:+92026480 None:intergenic
AGAGAAGGCAAATGCTCGTC+AGG 0.467028 7.4:-92026280 MS.gene54479:CDS
TGTGCCGTGACTCGTAGTAT+TGG 0.489174 7.4:+92026479 None:intergenic
GGCAAATGCTCGTCAGGAAA+GGG 0.498231 7.4:-92026274 MS.gene54479:CDS
TATATTTGCCCAATCAGGAC+AGG 0.517313 7.4:-92026420 MS.gene54479:CDS
GTGAGGCAATAAAACCTTGT+GGG 0.529652 7.4:+92026450 None:intergenic
AGGCAAATGCTCGTCAGGAA+AGG 0.543690 7.4:-92026275 MS.gene54479:CDS
TCAGCATGCTCCCACACATA+GGG 0.562374 7.4:+92026514 None:intergenic
ATCAAAAGTGTGGCCACAAG+TGG 0.562650 7.4:-92024951 MS.gene54479:CDS
ATCAGCATGCTCCCACACAT+AGG 0.581470 7.4:+92026513 None:intergenic
GGATGGCGACAATTTGGGCA+TGG 0.583454 7.4:+92025045 None:intergenic
TACAGGTTAAGATTAAGAGA+AGG 0.585633 7.4:-92026295 MS.gene54479:intron
TGTGGCCACAAGTGGAAGGA+GGG 0.590514 7.4:-92024943 MS.gene54479:CDS
TGTTATAACTAGCTTGAGGA+TGG 0.611096 7.4:+92025028 None:intergenic
GTGTGGCCACAAGTGGAAGG+AGG 0.613594 7.4:-92024944 MS.gene54479:CDS
AAAGTGTGGCCACAAGTGGA+AGG 0.625563 7.4:-92024947 MS.gene54479:CDS
ATATTTGCCCAATCAGGACA+GGG 0.626623 7.4:-92026419 MS.gene54479:CDS
TTACCCAATACTACGAGTCA+CGG 0.630549 7.4:-92026483 MS.gene54479:CDS
GGCAAATATATGGACAAGTG+AGG 0.649591 7.4:+92026433 None:intergenic
AAGAATAAACCAAAAGCCGA+CGG 0.660260 7.4:-92026218 MS.gene54479:intron
AGTGAGGCAATAAAACCTTG+TGG 0.661965 7.4:+92026449 None:intergenic
TGCATGTTATAACTAGCTTG+AGG 0.674563 7.4:+92025024 None:intergenic
GAGTGCATGAATCAAAAGTG+TGG 0.693654 7.4:-92024961 MS.gene54479:CDS
TATTTGCCCAATCAGGACAG+GGG 0.710769 7.4:-92026418 MS.gene54479:intron
ACGGCACATCTCATCCCACA+AGG 0.717130 7.4:-92026464 MS.gene54479:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAGTATTACAAAAATT+AGG + chr7.4:92026262-92026281 None:intergenic 15.0%
!! AAACAGTATTACAAAAATTA+GGG + chr7.4:92026261-92026280 None:intergenic 15.0%
!! TTAAACCTTGAAAATATATT+AGG + chr7.4:92025599-92025618 None:intergenic 15.0%
!!! AATCCAACTTTTTAAAAAAA+CGG + chr7.4:92026187-92026206 None:intergenic 15.0%
!!! TGTCTTTTTTTAATGATTTA+CGG - chr7.4:92026058-92026077 MS.gene54479:intron 15.0%
!! AACAGTATTACAAAAATTAG+GGG + chr7.4:92026260-92026279 None:intergenic 20.0%
!! AATTAACATTGTTTATGGTT+CGG - chr7.4:92025964-92025983 MS.gene54479:intron 20.0%
!! TAAACTCAAAAGTTTCTATT+AGG + chr7.4:92025850-92025869 None:intergenic 20.0%
!! TCTGAAGATAATCAATATTT+GGG - chr7.4:92025444-92025463 MS.gene54479:intron 20.0%
!!! TAACTAATTGGTAATAGAAA+TGG + chr7.4:92025102-92025121 None:intergenic 20.0%
!!! TAATACTGTTTTGTTCTTTT+TGG - chr7.4:92026270-92026289 MS.gene54479:CDS 20.0%
!!! TGTAATACTGTTTTTGTTAA+CGG - chr7.4:92026300-92026319 MS.gene54479:intron 20.0%
! AACTAGGAGGAAAAAAAAAA+GGG + chr7.4:92026399-92026418 None:intergenic 25.0%
! AAGAAAGAAAGATGAATTGA+AGG + chr7.4:92025129-92025148 None:intergenic 25.0%
! ATGGAAATAACAAAGAAACA+AGG + chr7.4:92026346-92026365 None:intergenic 25.0%
! ATTGAAGGTAACTAACTAAT+TGG + chr7.4:92025114-92025133 None:intergenic 25.0%
! CATATCAAATTTCTGTGATT+TGG - chr7.4:92025699-92025718 MS.gene54479:intron 25.0%
! CTTTAGTCAATAAGATAAGA+GGG + chr7.4:92025475-92025494 None:intergenic 25.0%
! GAGAAGGAAAAAAATAGTAA+TGG + chr7.4:92026365-92026384 None:intergenic 25.0%
! TCTTTAGTCAATAAGATAAG+AGG + chr7.4:92025476-92025495 None:intergenic 25.0%
! TTTAGTCAATAAGATAAGAG+GGG + chr7.4:92025474-92025493 None:intergenic 25.0%
!! AACTGATTGTACTAGTTTAT+TGG - chr7.4:92025383-92025402 MS.gene54479:intron 25.0%
!! CATGTAAAAGAAAAGCATAA+TGG + chr7.4:92025915-92025934 None:intergenic 25.0%
!! CTCTGAAGATAATCAATATT+TGG - chr7.4:92025443-92025462 MS.gene54479:intron 25.0%
!! GGTAACCTAATATATTTTCA+AGG - chr7.4:92025591-92025610 MS.gene54479:intron 25.0%
!!! ATTCTTGTTGATTTCATCAT+GGG + chr7.4:92025232-92025251 None:intergenic 25.0%
!!! CATCATGTTTTTTACTTTAC+AGG - chr7.4:92025152-92025171 MS.gene54479:intron 25.0%
!!! TATTCTTGTTGATTTCATCA+TGG + chr7.4:92025233-92025252 None:intergenic 25.0%
!!! TGTCCGTTTTTTTAAAAAGT+TGG - chr7.4:92026181-92026200 MS.gene54479:intron 25.0%
!!! TTATGGTTTTCTTATTTGAG+CGG - chr7.4:92026112-92026131 MS.gene54479:intron 25.0%
ACTAGGAGGAAAAAAAAAAG+GGG + chr7.4:92026398-92026417 None:intergenic 30.0%
ATCTAATGCAAAGGGATATA+TGG - chr7.4:92025517-92025536 MS.gene54479:intron 30.0%
ATTTCATCATGGGAGATAAT+AGG + chr7.4:92025222-92025241 None:intergenic 30.0%
CGACGAATTAACATTGTTTA+TGG - chr7.4:92025959-92025978 MS.gene54479:intron 30.0%
GAACTAGGAGGAAAAAAAAA+AGG + chr7.4:92026400-92026419 None:intergenic 30.0%
TACAGGTTAAGATTAAGAGA+AGG - chr7.4:92025169-92025188 MS.gene54479:intron 30.0%
TATCAGACAGCTTATATACA+TGG + chr7.4:92025664-92025683 None:intergenic 30.0%
TCGCGAATTAACATTGTTTA+TGG - chr7.4:92026095-92026114 MS.gene54479:intron 30.0%
TCTAATGCAAAGGGATATAT+GGG - chr7.4:92025518-92025537 MS.gene54479:intron 30.0%
TGAATGTGATCTAATGCAAA+GGG - chr7.4:92025509-92025528 MS.gene54479:intron 30.0%
TTACATGATTACTGTTTGAG+CGG - chr7.4:92025928-92025947 MS.gene54479:intron 30.0%
TTGAATGTGATCTAATGCAA+AGG - chr7.4:92025508-92025527 MS.gene54479:intron 30.0%
TTTCATCATGGGAGATAATA+GGG + chr7.4:92025221-92025240 None:intergenic 30.0%
! AAGTATTGCTCACTTAGATT+TGG + chr7.4:92025638-92025657 None:intergenic 30.0%
! TTATGAGCTTTTGTGAAGTA+GGG - chr7.4:92025754-92025773 MS.gene54479:intron 30.0%
! TTTATGAGCTTTTGTGAAGT+AGG - chr7.4:92025753-92025772 MS.gene54479:intron 30.0%
!! ATTTCTGTGATTTGGTTTCA+AGG - chr7.4:92025707-92025726 MS.gene54479:intron 30.0%
!!! TATTTTTTCTAGCACTGTTG+AGG - chr7.4:92025570-92025589 MS.gene54479:intron 30.0%
AAGAATAAACCAAAAGCCGA+CGG - chr7.4:92025246-92025265 MS.gene54479:intron 35.0%
AAGTAGGGAATTAGAAGTTG+TGG - chr7.4:92025769-92025788 MS.gene54479:intron 35.0%
CACTCGTAAAATATTGTTGC+AGG + chr7.4:92026488-92026507 None:intergenic 35.0%
CTAATGCAAAGGGATATATG+GGG - chr7.4:92025519-92025538 MS.gene54479:intron 35.0%
CTAGGAGGAAAAAAAAAAGG+GGG + chr7.4:92026397-92026416 None:intergenic 35.0%
GGTGATAGTTATTAGTCATG+TGG + chr7.4:92026239-92026258 None:intergenic 35.0%
! TCATTGTGGACTTGTTTACT+TGG - chr7.4:92025321-92025340 MS.gene54479:intron 35.0%
! TGAGTTTGCTTGTTTCAGTT+TGG - chr7.4:92025808-92025827 MS.gene54479:intron 35.0%
! TGCATGTTATAACTAGCTTG+AGG + chr7.4:92026443-92026462 None:intergenic 35.0%
! TGTACTAGTTTATTGGACGT+TGG - chr7.4:92025390-92025409 MS.gene54479:intron 35.0%
! TGTTATAACTAGCTTGAGGA+TGG + chr7.4:92026439-92026458 None:intergenic 35.0%
!!! GTTCAACCACTGTTTTGTTT+TGG - chr7.4:92026134-92026153 MS.gene54479:intron 35.0%
AGCAAGACAAACTTGGTACA+AGG + chr7.4:92025877-92025896 None:intergenic 40.0%
AGTCCACAATGAGCTAAAGA+AGG + chr7.4:92025313-92025332 None:intergenic 40.0%
AGTGAGGCAATAAAACCTTG+TGG + chr7.4:92025018-92025037 None:intergenic 40.0%
ATATTTGCCCAATCAGGACA+GGG - chr7.4:92025045-92025064 MS.gene54479:CDS 40.0%
CTCGAACCAAAACAAAACAG+TGG + chr7.4:92026143-92026162 None:intergenic 40.0%
GAGTGCATGAATCAAAAGTG+TGG - chr7.4:92026503-92026522 MS.gene54479:CDS 40.0%
GATGAACAGCAAGACAAACT+TGG + chr7.4:92025884-92025903 None:intergenic 40.0%
GGCAAATATATGGACAAGTG+AGG + chr7.4:92025034-92025053 None:intergenic 40.0%
GTCCATATATTTGCCCAATC+AGG - chr7.4:92025039-92025058 MS.gene54479:CDS 40.0%
GTCCTGATTGGGCAAATATA+TGG + chr7.4:92025044-92025063 None:intergenic 40.0%
GTGAGGCAATAAAACCTTGT+GGG + chr7.4:92025017-92025036 None:intergenic 40.0%
TATATTTGCCCAATCAGGAC+AGG - chr7.4:92025044-92025063 MS.gene54479:CDS 40.0%
TGTGAGAAAAGTAGTACCGT+CGG + chr7.4:92025265-92025284 None:intergenic 40.0%
TTACCCAATACTACGAGTCA+CGG - chr7.4:92024981-92025000 MS.gene54479:CDS 40.0%
! CATCCTTCTTTAGCTCATTG+TGG - chr7.4:92025307-92025326 MS.gene54479:intron 40.0%
! TGGGGCTTTAGTTTAGTGTA+AGG - chr7.4:92025537-92025556 MS.gene54479:intron 40.0%
!! CTGTGATTTGGTTTCAAGGA+CGG - chr7.4:92025711-92025730 MS.gene54479:intron 40.0%
!!! CTGTTTTGTTTTGGTTCGAG+CGG - chr7.4:92026143-92026162 MS.gene54479:intron 40.0%
AACATACCCCTGTCCTGATT+GGG + chr7.4:92025055-92025074 None:intergenic 45.0%
AATTTGGGCATGGAACTAGG+AGG + chr7.4:92026412-92026431 None:intergenic 45.0%
CTTGAGGATGGCGACAATTT+GGG + chr7.4:92026427-92026446 None:intergenic 45.0%
GACAATTTGGGCATGGAACT+AGG + chr7.4:92026415-92026434 None:intergenic 45.0%
GGGAATTAGAAGTTGTGGTC+TGG - chr7.4:92025774-92025793 MS.gene54479:intron 45.0%
TAACATACCCCTGTCCTGAT+TGG + chr7.4:92025056-92025075 None:intergenic 45.0%
TATTTGCCCAATCAGGACAG+GGG - chr7.4:92025046-92025065 MS.gene54479:CDS 45.0%
! ATCAAAAGTGTGGCCACAAG+TGG - chr7.4:92026513-92026532 MS.gene54479:CDS 45.0%
! ATGGAGTTTTGCCCTATGTG+TGG - chr7.4:92024939-92024958 MS.gene54479:CDS 45.0%
! GTTCAACCACCGTTTTGTCT+AGG - chr7.4:92025998-92026017 MS.gene54479:intron 45.0%
! TGGAGTTTTGCCCTATGTGT+GGG - chr7.4:92024940-92024959 MS.gene54479:CDS 45.0%
!!! AAAGTAGTACCGTCGGCTTT+TGG + chr7.4:92025258-92025277 None:intergenic 45.0%
AAAGTGTGGCCACAAGTGGA+AGG - chr7.4:92026517-92026536 MS.gene54479:CDS 50.0%
AAGGGGGAATGAGTGAGAGA+AGG + chr7.4:92026381-92026400 None:intergenic 50.0%
AGAGAAGGCAAATGCTCGTC+AGG - chr7.4:92025184-92025203 MS.gene54479:intron 50.0%
AGGCAAATGCTCGTCAGGAA+AGG - chr7.4:92025189-92025208 MS.gene54479:intron 50.0%
ATCAGCATGCTCCCACACAT+AGG + chr7.4:92024954-92024973 None:intergenic 50.0%
CCGCTCGAACCTAGACAAAA+CGG + chr7.4:92026010-92026029 None:intergenic 50.0%
CTCGAACCTAGACAAAACGG+TGG + chr7.4:92026007-92026026 None:intergenic 50.0%
GCTTGAGGATGGCGACAATT+TGG + chr7.4:92026428-92026447 None:intergenic 50.0%
GGCAAATGCTCGTCAGGAAA+GGG - chr7.4:92025190-92025209 MS.gene54479:intron 50.0%
GTGCCGTGACTCGTAGTATT+GGG + chr7.4:92024987-92025006 None:intergenic 50.0%
TCAGCATGCTCCCACACATA+GGG + chr7.4:92024953-92024972 None:intergenic 50.0%
TGTGCCGTGACTCGTAGTAT+TGG + chr7.4:92024988-92025007 None:intergenic 50.0%
! CCGTTTTGTCTAGGTTCGAG+CGG - chr7.4:92026007-92026026 MS.gene54479:intron 50.0%
ACGGCACATCTCATCCCACA+AGG - chr7.4:92025000-92025019 MS.gene54479:CDS 55.0%
GGATGGCGACAATTTGGGCA+TGG + chr7.4:92026422-92026441 None:intergenic 55.0%
TGTGGCCACAAGTGGAAGGA+GGG - chr7.4:92026521-92026540 MS.gene54479:CDS 55.0%
GTGTGGCCACAAGTGGAAGG+AGG - chr7.4:92026520-92026539 MS.gene54479:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 92024939 92026547 92024939 ID=MS.gene54479
chr7.4 mRNA 92024939 92026547 92024939 ID=MS.gene54479.t1;Parent=MS.gene54479
chr7.4 exon 92026419 92026547 92026419 ID=MS.gene54479.t1.exon1;Parent=MS.gene54479.t1
chr7.4 CDS 92026419 92026547 92026419 ID=cds.MS.gene54479.t1;Parent=MS.gene54479.t1
chr7.4 exon 92026219 92026312 92026219 ID=MS.gene54479.t1.exon2;Parent=MS.gene54479.t1
chr7.4 CDS 92026219 92026312 92026219 ID=cds.MS.gene54479.t1;Parent=MS.gene54479.t1
chr7.4 exon 92024939 92025069 92024939 ID=MS.gene54479.t1.exon3;Parent=MS.gene54479.t1
chr7.4 CDS 92024939 92025069 92024939 ID=cds.MS.gene54479.t1;Parent=MS.gene54479.t1
Gene Sequence

>MS.gene54479

ATGGAGTTTTGCCCTATGTGTGGGAGCATGCTGATGCTTGAATTACCCAATACTACGAGTCACGGCACATCTCATCCCACAAGGTTTTATTGCCTCACTTGTCCATATATTTGCCCAATCAGGACAGGGGTATGTTATGTACTCGAGTCGAGTCTATTTTCCATTTCTATTACCAATTAGTTAGTTACCTTCAATTCATCTTTCTTTCTTTATCATCATGTTTTTTACTTTACAGGTTAAGATTAAGAGAAGGCAAATGCTCGTCAGGAAAGGGATTGACCCTATTATCTCCCATGATGAAATCAACAAGAATAAACCAAAAGCCGACGGTACTACTTTTCTCACAATTTATCTCAATTTTTGTTAATCATCCTTCTTTAGCTCATTGTGGACTTGTTTACTTGGATAATCAAATATTTGTGAAATGATTATATCAATTGTCTGAACTGATTGTACTAGTTTATTGGACGTTGGAGATGATACAATCTGTTTTTAGTGATTCGACTCTGAAGATAATCAATATTTGGGATTACCCCTCTTATCTTATTGACTAAAGACTTTCTCTTGAGTTGAATGTGATCTAATGCAAAGGGATATATGGGGCTTTAGTTTAGTGTAAGGCAAGCTTGTTTATTTTTTCTAGCACTGTTGAGGTAACCTAATATATTTTCAAGGTTTAAATGTCAAAATGTGAAGCCAAATCTAAGTGAGCAATACTTTTGCCATGTATATAAGCTGTCTGATATAGCTTAATTTTTAACATATCAAATTTCTGTGATTTGGTTTCAAGGACGGTGTGCTCAAAGCTATAATATTTATGAGCTTTTGTGAAGTAGGGAATTAGAAGTTGTGGTCTGGTTTTGTTTGTGTGAGTTTGCTTGTTTCAGTTTGGATTATCAATATCAACTCCTAATAGAAACTTTTGAGTTTATTTTCCTTGTACCAAGTTTGTCTTGCTGTTCATCATGTGATTCCATTATGCTTTTCTTTTACATGATTACTGTTTGAGCGGTTGAATCACGACGAATTAACATTGTTTATGGTTCGGTTATCTGAGCAGTTCAACCACCGTTTTGTCTAGGTTCGAGCGGTTTAAAACAGTTGAATCAATGATTTAGATGTCTTTTTTTAATGATTTACGGTTTGAGCAGTTGAATCGCGAATTAACATTGTTTATGGTTTTCTTATTTGAGCGGTTCAACCACTGTTTTGTTTTGGTTCGAGCGGTTTAAAACTGCTTGATGTCCGTTTTTTTAAAAAGTTGGATTGATTTTGTCTTTGTATGTCTTCTTTCAGACCACATGACTAATAACTATCACCCCTAATTTTTGTAATACTGTTTTGTTCTTTTTGGTACAATTTGTAATACTGTTTTTGTTAACGGATATTGAGTAATTATGCAATCCTTGTTTCTTTGTTATTTCCATTACTATTTTTTTCCTTCTCTCACTCATTCCCCCTTTTTTTTTTCCTCCTAGTTCCATGCCCAAATTGTCGCCATCCTCAAGCTAGTTATAACATGCAACAGACTCGATCAGCTGATGAACCTGCAACAATATTTTACGAGTGCATGAATCAAAAGTGTGGCCACAAGTGGAAGGAGGGATAA

Protein sequence

>MS.gene54479.t1

MEFCPMCGSMLMLELPNTTSHGTSHPTRFYCLTCPYICPIRTGVKIKRRQMLVRKGIDPIISHDEINKNKPKADVPCPNCRHPQASYNMQQTRSADEPATIFYECMNQKCGHKWKEG