Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55063.t1 | XP_013449411.1 | 98.4 | 321 | 5 | 0 | 1 | 321 | 1 | 321 | 2.30E-180 | 641.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55063.t1 | Q8VY08 | 73.5 | 317 | 75 | 2 | 5 | 321 | 1 | 308 | 5.9e-132 | 471.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55063.t1 | A0A072U137 | 98.4 | 321 | 5 | 0 | 1 | 321 | 1 | 321 | 1.7e-180 | 641.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene55063 | 0.80612 | 9.60E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene55063 | 0.816896 | 4.36E-52 | -1.69E-46 |
| MS.gene049861 | MS.gene55063 | 0.808125 | 3.61E-50 | -1.69E-46 |
| MS.gene050001 | MS.gene55063 | 0.826191 | 3.11E-54 | -1.69E-46 |
| MS.gene050114 | MS.gene55063 | 0.838578 | 2.65E-57 | -1.69E-46 |
| MS.gene050317 | MS.gene55063 | 0.810474 | 1.13E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene55063 | 0.83406 | 3.73E-56 | -1.69E-46 |
| MS.gene051748 | MS.gene55063 | 0.803539 | 3.32E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene55063 | 0.819493 | 1.13E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene55063 | 0.80739 | 5.17E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene55063 | 0.817274 | 3.59E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene55063 | 0.846511 | 2.07E-59 | -1.69E-46 |
| MS.gene052434 | MS.gene55063 | 0.83061 | 2.67E-55 | -1.69E-46 |
| MS.gene052533 | MS.gene55063 | 0.803301 | 3.72E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene55063 | -0.817621 | 3.00E-52 | -1.69E-46 |
| MS.gene052932 | MS.gene55063 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene55063 | 0.805172 | 1.52E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene55063 | 0.835348 | 1.77E-56 | -1.69E-46 |
| MS.gene053340 | MS.gene55063 | 0.802147 | 6.44E-49 | -1.69E-46 |
| MS.gene053952 | MS.gene55063 | 0.815349 | 9.68E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene55063 | 0.845006 | 5.31E-59 | -1.69E-46 |
| MS.gene054222 | MS.gene55063 | 0.805746 | 1.15E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene55063 | 0.804778 | 1.83E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene55063 | 0.816391 | 5.67E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene55063 | 0.804472 | 2.13E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene55063 | 0.812523 | 4.06E-51 | -1.69E-46 |
| MS.gene055314 | MS.gene55063 | 0.807981 | 3.88E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene55063 | 0.806201 | 9.23E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene55063 | 0.850369 | 1.78E-60 | -1.69E-46 |
| MS.gene056181 | MS.gene55063 | 0.80711 | 5.93E-50 | -1.69E-46 |
| MS.gene056403 | MS.gene55063 | 0.868062 | 8.63E-66 | -1.69E-46 |
| MS.gene056605 | MS.gene55063 | 0.860053 | 2.70E-63 | -1.69E-46 |
| MS.gene056638 | MS.gene55063 | 0.810326 | 1.22E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene55063 | 0.800632 | 1.32E-48 | -1.69E-46 |
| MS.gene056986 | MS.gene55063 | 0.855712 | 5.25E-62 | -1.69E-46 |
| MS.gene057094 | MS.gene55063 | 0.819019 | 1.45E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene55063 | 0.829359 | 5.38E-55 | -1.69E-46 |
| MS.gene057155 | MS.gene55063 | 0.802814 | 4.69E-49 | -1.69E-46 |
| MS.gene057360 | MS.gene55063 | 0.821234 | 4.50E-53 | -1.69E-46 |
| MS.gene05781 | MS.gene55063 | 0.840228 | 9.87E-58 | -1.69E-46 |
| MS.gene057999 | MS.gene55063 | 0.852289 | 5.09E-61 | -1.69E-46 |
| MS.gene058074 | MS.gene55063 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene55063 | 0.815213 | 1.04E-51 | -1.69E-46 |
| MS.gene058897 | MS.gene55063 | 0.804601 | 2.00E-49 | -1.69E-46 |
| MS.gene059144 | MS.gene55063 | 0.801366 | 9.31E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene55063 | 0.808755 | 2.65E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene55063 | 0.802727 | 4.89E-49 | -1.69E-46 |
| MS.gene059830 | MS.gene55063 | 0.808132 | 3.60E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene55063 | -0.807631 | 4.60E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene55063 | 0.800425 | 1.45E-48 | -1.69E-46 |
| MS.gene06011 | MS.gene55063 | 0.853666 | 2.05E-61 | -1.69E-46 |
| MS.gene060365 | MS.gene55063 | 0.802626 | 5.13E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene55063 | 0.82053 | 6.53E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene55063 | 0.803099 | 4.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55063.t1 | MTR_7g081715 | 98.442 | 321 | 5 | 0 | 1 | 321 | 1 | 321 | 0.0 | 660 |
| MS.gene55063.t1 | MTR_7g081715 | 98.377 | 308 | 5 | 0 | 1 | 308 | 1 | 308 | 0.0 | 633 |
| MS.gene55063.t1 | MTR_7g081715 | 98.052 | 308 | 5 | 1 | 1 | 308 | 1 | 307 | 0.0 | 627 |
| MS.gene55063.t1 | MTR_7g081715 | 98.282 | 291 | 5 | 0 | 1 | 291 | 1 | 291 | 0.0 | 595 |
| MS.gene55063.t1 | MTR_7g081715 | 97.611 | 293 | 6 | 1 | 1 | 293 | 1 | 292 | 0.0 | 591 |
| MS.gene55063.t1 | MTR_7g081715 | 97.938 | 291 | 5 | 1 | 1 | 291 | 1 | 290 | 0.0 | 590 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55063.t1 | AT1G02100 | 73.502 | 317 | 75 | 2 | 5 | 321 | 1 | 308 | 3.26e-176 | 491 |
| MS.gene55063.t1 | AT1G02100 | 73.502 | 317 | 75 | 2 | 5 | 321 | 1 | 308 | 3.26e-176 | 491 |
| MS.gene55063.t1 | AT1G02100 | 73.026 | 304 | 73 | 2 | 5 | 308 | 1 | 295 | 2.01e-165 | 462 |
Find 68 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGTATCTGCGTCTTTAT+TGG | 0.202675 | 7.3:-25805839 | None:intergenic |
| AATAATACCTGCGTCTTTCT+TGG | 0.274176 | 7.3:-25813646 | None:intergenic |
| CATCCAGGTAACAAGTAAAA+AGG | 0.327516 | 7.3:+25807396 | MS.gene55063:intron |
| TTGTAGGTCATGTGTGAAAA+AGG | 0.332658 | 7.3:+25805571 | MS.gene55063:intron |
| ATAGTATCTGCGTCTTTATT+GGG | 0.334293 | 7.3:-25805838 | None:intergenic |
| ATAATCGGAGATCTTCTTAC+AGG | 0.352376 | 7.3:-25805635 | None:intergenic |
| ATCCATCCAGATGATGCTTT+TGG | 0.356856 | 7.3:+25809113 | MS.gene55063:CDS |
| ACAACTTCGTGACTTGAATC+TGG | 0.377061 | 7.3:-25808951 | None:intergenic |
| GAAACTGGTAGAGAAATTTC+CGG | 0.393824 | 7.3:-25805784 | None:intergenic |
| ATAATACCTGCGTCTTTCTT+GGG | 0.402004 | 7.3:-25813645 | None:intergenic |
| TGAAGGTTACTTTGCTCGTT+GGG | 0.405577 | 7.3:+25805754 | MS.gene55063:intron |
| GCAGATGATCAGAAATTTAG+AGG | 0.407200 | 7.3:+25809139 | MS.gene55063:CDS |
| TGCTGACCAAAAGCATCATC+TGG | 0.407645 | 7.3:-25809119 | None:intergenic |
| CGTGCGAGCGTACAATTTAC+TGG | 0.413604 | 7.3:-25804466 | None:intergenic |
| ACTGTTTAGGATTGAACGTC+TGG | 0.413688 | 7.3:+25816322 | MS.gene55063:intron |
| ATCTTACCACGATTAATAAT+CGG | 0.432639 | 7.3:-25805650 | None:intergenic |
| GTGAAACAGCCTCCATTTCA+AGG | 0.435679 | 7.3:+25807461 | MS.gene55063:CDS |
| AAGTCACGAAGTTGTTGGTT+GGG | 0.441637 | 7.3:+25808959 | MS.gene55063:CDS |
| TTTATATCACGCAAGTCTGC+AGG | 0.460139 | 7.3:-25807952 | None:intergenic |
| GGCGGAAGCGTCGTCGTTGG+TGG | 0.461549 | 7.3:-25804445 | None:intergenic |
| ATGAAGGTTACTTTGCTCGT+TGG | 0.462272 | 7.3:+25805753 | MS.gene55063:intron |
| CATTCTGCAATTATAAAAGT+TGG | 0.463383 | 7.3:-25808909 | None:intergenic |
| GCACTACTTACCCCTTGAAA+TGG | 0.465292 | 7.3:-25807473 | None:intergenic |
| GATTCAAGTCACGAAGTTGT+TGG | 0.467158 | 7.3:+25808954 | MS.gene55063:CDS |
| CATGAAATACCTGCCATCCT+TGG | 0.472289 | 7.3:-25810643 | None:intergenic |
| ACTGTTGTCGATGCGTGAAT+CGG | 0.479296 | 7.3:-25804403 | None:intergenic |
| TGCTGTTGAAAATGTTCGGC+AGG | 0.483957 | 7.3:-25808982 | None:intergenic |
| TTGCTCGTTGGGCTGCCCTC+CGG | 0.486240 | 7.3:+25805765 | MS.gene55063:CDS |
| CAAGTCACGAAGTTGTTGGT+TGG | 0.496700 | 7.3:+25808958 | MS.gene55063:CDS |
| ACAGTGCTGTTGAAAATGTT+CGG | 0.499849 | 7.3:-25808986 | None:intergenic |
| CTGCAATTATAAAAGTTGGC+AGG | 0.510055 | 7.3:-25808905 | None:intergenic |
| ACATTCATTTATTTGTGAGA+AGG | 0.526997 | 7.3:+25805612 | MS.gene55063:CDS |
| CCTTGGATCCATCTCAGTAA+TGG | 0.532406 | 7.3:-25807995 | None:intergenic |
| GTGTCGCGGATGTCCTCTGT+TGG | 0.532985 | 7.3:+25810571 | MS.gene55063:intron |
| TGTCGCGGATGTCCTCTGTT+GGG | 0.539944 | 7.3:+25810572 | MS.gene55063:CDS |
| GACCAAAAGCATCATCTGGA+TGG | 0.540152 | 7.3:-25809115 | None:intergenic |
| TACACCAACTTTACATGCAA+AGG | 0.540339 | 7.3:+25810604 | MS.gene55063:CDS |
| CATCATAACCATTACTGAGA+TGG | 0.545001 | 7.3:+25807987 | MS.gene55063:CDS |
| AGTCAATTGAAGAATAAACT+TGG | 0.549546 | 7.3:+25807439 | MS.gene55063:CDS |
| AGAATGTGTTCTGATTTACT+TGG | 0.566293 | 7.3:+25808926 | MS.gene55063:CDS |
| TGTGGAAGTGGACTTTAAAG+AGG | 0.570011 | 7.3:+25807124 | MS.gene55063:CDS |
| TGAAACAGCCTCCATTTCAA+GGG | 0.572201 | 7.3:+25807462 | MS.gene55063:CDS |
| ACCACATTTATATGTGGAAG+TGG | 0.576623 | 7.3:+25807112 | MS.gene55063:CDS |
| ACTGGCGGAAGCGTCGTCGT+TGG | 0.577080 | 7.3:-25804448 | None:intergenic |
| GTATCAGAAGTCTGTTGCAT+GGG | 0.583694 | 7.3:+25813589 | MS.gene55063:intron |
| CTACTTACCCCTTGAAATGG+AGG | 0.585507 | 7.3:-25807470 | None:intergenic |
| CAGATACTATCACTTGGAGC+AGG | 0.588756 | 7.3:+25805851 | MS.gene55063:CDS |
| TGAAGAGTGGTACATGATGC+AGG | 0.593224 | 7.3:+25816361 | MS.gene55063:CDS |
| GAAAGCACCACATTTATATG+TGG | 0.593298 | 7.3:+25807106 | MS.gene55063:CDS |
| TTCAACCATGGCGGATACAG+TGG | 0.595052 | 7.3:+25804380 | None:intergenic |
| TTATATCACGCAAGTCTGCA+GGG | 0.602424 | 7.3:-25807951 | None:intergenic |
| ATCGGCCACTGTATCCGCCA+TGG | 0.603474 | 7.3:-25804385 | None:intergenic |
| GGTATCAGAAGTCTGTTGCA+TGG | 0.607722 | 7.3:+25813588 | MS.gene55063:intron |
| CCATTACTGAGATGGATCCA+AGG | 0.618047 | 7.3:+25807995 | MS.gene55063:CDS |
| TTGATGCCCAAGAAAGACGC+AGG | 0.618119 | 7.3:+25813639 | MS.gene55063:CDS |
| TTATGCAATCAATGATGCAA+TGG | 0.620946 | 7.3:+25816667 | MS.gene55063:CDS |
| AAGACGCAGATACTATCACT+TGG | 0.623489 | 7.3:+25805845 | MS.gene55063:CDS |
| TGTTCGATGAATTTGAAGAG+TGG | 0.629354 | 7.3:+25816348 | MS.gene55063:CDS |
| TCCACTTCCACATATAAATG+TGG | 0.634696 | 7.3:-25807113 | None:intergenic |
| AGATCTCCGATTATTAATCG+TGG | 0.658165 | 7.3:+25805644 | MS.gene55063:CDS |
| ATTTCAGGAGCACTACTGTG+TGG | 0.662705 | 7.3:+25816643 | MS.gene55063:intron |
| GAAACAGCCTCCATTTCAAG+GGG | 0.674160 | 7.3:+25807463 | MS.gene55063:CDS |
| CTGGTAGAGAAATTTCCGGA+GGG | 0.686441 | 7.3:-25805780 | None:intergenic |
| ACTGGTAGAGAAATTTCCGG+AGG | 0.699047 | 7.3:-25805781 | None:intergenic |
| CGACAACAGTAACACTGCGG+CGG | 0.708713 | 7.3:+25804416 | MS.gene55063:CDS |
| GCGAGCGTACAATTTACTGG+CGG | 0.759993 | 7.3:-25804463 | None:intergenic |
| GTAGCATAGATACCCAACAG+AGG | 0.778818 | 7.3:-25810584 | None:intergenic |
| CATCGACAACAGTAACACTG+CGG | 0.817282 | 7.3:+25804413 | MS.gene55063:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25804387 | 25816688 | 25804387 | ID=MS.gene55063 |
| chr7.3 | mRNA | 25804387 | 25816688 | 25804387 | ID=MS.gene55063.t1;Parent=MS.gene55063 |
| chr7.3 | exon | 25804387 | 25804475 | 25804387 | ID=MS.gene55063.t1.exon1;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25804387 | 25804475 | 25804387 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25805577 | 25805665 | 25805577 | ID=MS.gene55063.t1.exon2;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25805577 | 25805665 | 25805577 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25805759 | 25805901 | 25805759 | ID=MS.gene55063.t1.exon3;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25805759 | 25805901 | 25805759 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25807098 | 25807145 | 25807098 | ID=MS.gene55063.t1.exon4;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25807098 | 25807145 | 25807098 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25807403 | 25807484 | 25807403 | ID=MS.gene55063.t1.exon5;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25807403 | 25807484 | 25807403 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25807914 | 25808016 | 25807914 | ID=MS.gene55063.t1.exon6;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25807914 | 25808016 | 25807914 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25808905 | 25809025 | 25808905 | ID=MS.gene55063.t1.exon7;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25808905 | 25809025 | 25808905 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25809113 | 25809160 | 25809113 | ID=MS.gene55063.t1.exon8;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25809113 | 25809160 | 25809113 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25810572 | 25810655 | 25810572 | ID=MS.gene55063.t1.exon9;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25810572 | 25810655 | 25810572 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25813596 | 25813660 | 25813596 | ID=MS.gene55063.t1.exon10;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25813596 | 25813660 | 25813596 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25816331 | 25816382 | 25816331 | ID=MS.gene55063.t1.exon11;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25816331 | 25816382 | 25816331 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| chr7.3 | exon | 25816650 | 25816688 | 25816650 | ID=MS.gene55063.t1.exon12;Parent=MS.gene55063.t1 |
| chr7.3 | CDS | 25816650 | 25816688 | 25816650 | ID=cds.MS.gene55063.t1;Parent=MS.gene55063.t1 |
| Gene Sequence |
| Protein sequence |
