AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050031


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050031.t1 MTR_6g015010 73.810 126 15 1 1 108 167 292 2.59e-44 152
MS.gene050031.t1 MTR_6g015010 89.286 56 6 0 107 162 363 418 4.68e-25 100
MS.gene050031.t1 MTR_7g013070 67.857 56 18 0 107 162 334 389 3.89e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050031.t1 AT5G15510 64.286 56 20 0 107 162 369 424 7.40e-17 77.0
MS.gene050031.t1 AT5G15510 38.261 115 63 3 1 108 186 299 4.02e-12 63.5
MS.gene050031.t1 AT5G15510 64.286 56 20 0 107 162 369 424 7.40e-17 77.0
MS.gene050031.t1 AT5G15510 38.261 115 63 3 1 108 186 299 4.02e-12 63.5
MS.gene050031.t1 AT5G15510 64.286 56 20 0 107 162 369 424 8.74e-17 77.0
MS.gene050031.t1 AT5G15510 38.261 115 63 3 1 108 186 299 3.04e-12 63.9
MS.gene050031.t1 AT5G15510 64.286 56 20 0 107 162 369 424 1.00e-16 76.6
MS.gene050031.t1 AT5G15510 38.261 115 63 3 1 108 186 299 4.09e-12 63.5
MS.gene050031.t1 AT3G01015 63.636 55 20 0 108 162 360 414 1.02e-15 73.9
MS.gene050031.t1 AT3G01015 38.655 119 60 2 1 108 173 289 7.96e-12 62.8

Find 38 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGTTACTTAGTATAAAA+AGG 0.150829 5.4:-51216547 MS.gene050031:CDS
AGGAATAGTATACCTGAAAA+TGG 0.240990 5.4:-51216599 MS.gene050031:CDS
GAGCAATCTGAAGTTGTTTC+TGG 0.276694 5.4:-51216419 MS.gene050031:CDS
TGCTAGTTTGTTTACTGCTT+GGG 0.315351 5.4:-51216381 MS.gene050031:CDS
TCCCTTACAGTTGGCTGAAT+AGG 0.336982 5.4:-51214833 None:intergenic
AGAGAATGAGAAGAAGAATA+AGG 0.338941 5.4:-51216498 MS.gene050031:CDS
TGCACCTGATGATCAAATTC+CGG 0.346673 5.4:+51215088 None:intergenic
AGAAGAATAAGGTGATGAAA+TGG 0.355774 5.4:-51216487 MS.gene050031:CDS
GCACCTGATGATCAAATTCC+GGG 0.368237 5.4:+51215089 None:intergenic
GAAGAATAAGGTGATGAAAT+GGG 0.371425 5.4:-51216486 MS.gene050031:CDS
TGCTCATCTTCATCCTCAAC+AGG 0.390012 5.4:+51216437 None:intergenic
GTGTGTAGCAGGAACTCTTC+TGG 0.394499 5.4:-51216208 MS.gene050031:CDS
AGAACAACAGAAAAGGGAAT+TGG 0.400583 5.4:-51214965 MS.gene050031:CDS
ATGCTAGTTTGTTTACTGCT+TGG 0.408757 5.4:-51216382 MS.gene050031:CDS
AGGCTTTGTGCTTTCTTTCA+CGG 0.409057 5.4:+51215153 None:intergenic
CTATGAAGCTTCACATCAAT+AGG 0.425787 5.4:+51215133 None:intergenic
GGCTTTGTGCTTTCTTTCAC+GGG 0.443138 5.4:+51215154 None:intergenic
TCGCCCGGAATTTGATCATC+AGG 0.444522 5.4:-51215092 MS.gene050031:intron
GGGATTGTGGAAGTGGAAGA+AGG 0.460575 5.4:-51216361 MS.gene050031:intron
GGAATTGGAAATACAACAAA+AGG 0.463028 5.4:-51214950 MS.gene050031:intron
GGAATAGTATACCTGAAAAT+GGG 0.472624 5.4:-51216598 MS.gene050031:CDS
TTGTTTACTGCTTGGGATTG+TGG 0.473847 5.4:-51216374 MS.gene050031:CDS
TTTGATAGAACAACAGAAAA+GGG 0.474688 5.4:-51214971 MS.gene050031:CDS
GTTTGATAGAACAACAGAAA+AGG 0.481566 5.4:-51214972 MS.gene050031:CDS
GAAAGAGAAGAATGAGGATG+AGG 0.486091 5.4:-51216522 MS.gene050031:CDS
GTGATGAAATGGGGATTACA+TGG 0.497700 5.4:-51216476 MS.gene050031:CDS
GAAGCTTCATAGTGATTTGA+GGG 0.498697 5.4:-51215122 MS.gene050031:CDS
TGAAGCTTCATAGTGATTTG+AGG 0.503817 5.4:-51215123 MS.gene050031:CDS
TTTGAGGGCAATTGATCGCC+CGG 0.517154 5.4:-51215107 MS.gene050031:CDS
ACTGCTTGGGATTGTGGAAG+TGG 0.521266 5.4:-51216368 MS.gene050031:CDS
AAATGGGAAAGTGATGCATT+TGG 0.528141 5.4:-51216582 MS.gene050031:CDS
GCAATTTCAACAACCTGTTG+AGG 0.537406 5.4:-51216450 MS.gene050031:CDS
AGAGAGTAATGGAAGAAGCA+AGG 0.549720 5.4:-51216619 None:intergenic
AAGAATAAGGTGATGAAATG+GGG 0.564108 5.4:-51216485 MS.gene050031:CDS
AAGGGAGAAAGAGAAGAATG+AGG 0.575728 5.4:-51216528 MS.gene050031:CDS
GAAAGTGATGCATTTGGTGA+AGG 0.605917 5.4:-51216576 MS.gene050031:CDS
TTTGTGCTTTCTTTCACGGG+AGG 0.620777 5.4:+51215157 None:intergenic
TGTAGCAGGAACTCTTCTGG+TGG 0.669446 5.4:-51216205 MS.gene050031:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAATTCTTTACATTGA+CGG - chr5.4:51215848-51215867 MS.gene050031:intron 15.0%
!! AAAACTTTCAGTTTATTTGA+TGG - chr5.4:51215807-51215826 MS.gene050031:intron 20.0%
!! ACTATAATACTAATGAAGAT+TGG - chr5.4:51215424-51215443 MS.gene050031:intron 20.0%
!! CAAATTTATCATAATTCTGT+TGG - chr5.4:51216213-51216232 MS.gene050031:intron 20.0%
! AGAGGTTACTTAGTATAAAA+AGG - chr5.4:51214898-51214917 MS.gene050031:intron 25.0%
! ATAGCAATTCTCAATTAGAA+CGG - chr5.4:51215987-51216006 MS.gene050031:intron 25.0%
! ATGCTAGGATATTAGATATA+AGG + chr5.4:51215953-51215972 None:intergenic 25.0%
! GAGGTTACTTAGTATAAAAA+GGG - chr5.4:51214899-51214918 MS.gene050031:intron 25.0%
! TCATGATGTGTAAAACTAAA+GGG + chr5.4:51216405-51216424 None:intergenic 25.0%
! TCATTTCTGCAAAATGTTAT+CGG - chr5.4:51215375-51215394 MS.gene050031:intron 25.0%
! TGTATTTCCATTTGTGATTA+AGG - chr5.4:51215311-51215330 MS.gene050031:intron 25.0%
! TTAGTGATTCACTATTAACA+AGG - chr5.4:51215447-51215466 MS.gene050031:intron 25.0%
! TTCATGATGTGTAAAACTAA+AGG + chr5.4:51216406-51216425 None:intergenic 25.0%
! TTTGATAGAACAACAGAAAA+GGG - chr5.4:51216474-51216493 MS.gene050031:CDS 25.0%
!! CTTTTAGATAAAGGAAAACA+TGG + chr5.4:51216575-51216594 None:intergenic 25.0%
!!! GTAATTTGTTAATCAAGCAT+AGG - chr5.4:51215599-51215618 MS.gene050031:intron 25.0%
AAACATGGCAACTCAAAATT+AGG + chr5.4:51216560-51216579 None:intergenic 30.0%
AAGAATAAGGTGATGAAATG+GGG - chr5.4:51214960-51214979 MS.gene050031:CDS 30.0%
AGAAGAATAAGGTGATGAAA+TGG - chr5.4:51214958-51214977 MS.gene050031:CDS 30.0%
AGAGAATGAGAAGAAGAATA+AGG - chr5.4:51214947-51214966 MS.gene050031:intron 30.0%
AGCATATACACTTCAAACTA+AGG + chr5.4:51215525-51215544 None:intergenic 30.0%
AGGAATAGTATACCTGAAAA+TGG - chr5.4:51214846-51214865 MS.gene050031:intron 30.0%
ATCGTAATTGCATTACATGA+TGG + chr5.4:51215669-51215688 None:intergenic 30.0%
CATGATGTGTAAAACTAAAG+GGG + chr5.4:51216404-51216423 None:intergenic 30.0%
CTTTATCTAAAAGTTGACGT+CGG - chr5.4:51216582-51216601 MS.gene050031:CDS 30.0%
GAAGAATAAGGTGATGAAAT+GGG - chr5.4:51214959-51214978 MS.gene050031:CDS 30.0%
GAATTAAATCTGTGAGTAGT+TGG - chr5.4:51215878-51215897 MS.gene050031:intron 30.0%
GGAATAGTATACCTGAAAAT+GGG - chr5.4:51214847-51214866 MS.gene050031:intron 30.0%
GGAATTGGAAATACAACAAA+AGG - chr5.4:51216495-51216514 MS.gene050031:CDS 30.0%
GTTTGATAGAACAACAGAAA+AGG - chr5.4:51216473-51216492 MS.gene050031:CDS 30.0%
TAACAACTCACAGTCAAATT+TGG - chr5.4:51215548-51215567 MS.gene050031:intron 30.0%
TCGTAATTGCATTACATGAT+GGG + chr5.4:51215668-51215687 None:intergenic 30.0%
TCTAATATCCTAGCATAACA+TGG - chr5.4:51215957-51215976 MS.gene050031:intron 30.0%
TTACGATTATAGCTTATGTC+TGG - chr5.4:51215682-51215701 MS.gene050031:intron 30.0%
AAATGGGAAAGTGATGCATT+TGG - chr5.4:51214863-51214882 MS.gene050031:intron 35.0%
ACTATTAACAAGGTCTTCCA+TGG - chr5.4:51215457-51215476 MS.gene050031:intron 35.0%
AGAACAACAGAAAAGGGAAT+TGG - chr5.4:51216480-51216499 MS.gene050031:CDS 35.0%
AGACTCAAGAGGATGTTTAT+TGG + chr5.4:51216081-51216100 None:intergenic 35.0%
ATTTGTGATTAAGGCATGTC+CGG - chr5.4:51215320-51215339 MS.gene050031:intron 35.0%
CTATGAAGCTTCACATCAAT+AGG + chr5.4:51216315-51216334 None:intergenic 35.0%
CTGTAACAGAAGAATGCTAA+TGG + chr5.4:51216256-51216275 None:intergenic 35.0%
GAAGCTTCATAGTGATTTGA+GGG - chr5.4:51216323-51216342 MS.gene050031:intron 35.0%
GGTTTATATTCCCTTACAGT+TGG - chr5.4:51216603-51216622 MS.gene050031:CDS 35.0%
TAACGCAGCAACATCATTAA+GGG + chr5.4:51216054-51216073 None:intergenic 35.0%
TCAATTAGAACGGTACATTG+TGG - chr5.4:51215997-51216016 MS.gene050031:intron 35.0%
TGAAGCTTCATAGTGATTTG+AGG - chr5.4:51216322-51216341 MS.gene050031:intron 35.0%
TGATGAATGCTTAACTGTGT+AGG - chr5.4:51216435-51216454 MS.gene050031:CDS 35.0%
TGGAGTTAATTCAGTTTCAG+TGG - chr5.4:51216121-51216140 MS.gene050031:intron 35.0%
TTAACGCAGCAACATCATTA+AGG + chr5.4:51216055-51216074 None:intergenic 35.0%
! ATAAATTTTCACGCACTGTC+AGG - chr5.4:51215714-51215733 MS.gene050031:intron 35.0%
! CGACGTCAACTTTTAGATAA+AGG + chr5.4:51216584-51216603 None:intergenic 35.0%
! CTGATTTGAGATGTGAAAGT+TGG + chr5.4:51216532-51216551 None:intergenic 35.0%
! TAAATTTTCACGCACTGTCA+GGG - chr5.4:51215715-51215734 MS.gene050031:intron 35.0%
!! ATGCTAGTTTGTTTACTGCT+TGG - chr5.4:51215063-51215082 MS.gene050031:intron 35.0%
!! ATTTGGTGAAGGTTTTTGAG+AGG - chr5.4:51214880-51214899 MS.gene050031:intron 35.0%
!! GATGTGAAAGTTGGTAAACA+TGG + chr5.4:51216523-51216542 None:intergenic 35.0%
!! TGCTAGTTTGTTTACTGCTT+GGG - chr5.4:51215064-51215083 MS.gene050031:intron 35.0%
AAGGGAGAAAGAGAAGAATG+AGG - chr5.4:51214917-51214936 MS.gene050031:intron 40.0%
ACATATGGTCAAGACTCAAG+AGG + chr5.4:51216092-51216111 None:intergenic 40.0%
ACTCCATATCAGCTGACATA+TGG + chr5.4:51216107-51216126 None:intergenic 40.0%
ACTGATCATGTGTATGACTC+TGG - chr5.4:51215765-51215784 MS.gene050031:intron 40.0%
ATCAACGTGTTAGTGACATG+CGG - chr5.4:51215399-51215418 MS.gene050031:intron 40.0%
CGATTATAGCTTATGTCTGG+TGG - chr5.4:51215685-51215704 MS.gene050031:intron 40.0%
GAAAGAGAAGAATGAGGATG+AGG - chr5.4:51214923-51214942 MS.gene050031:intron 40.0%
GCAATTTCAACAACCTGTTG+AGG - chr5.4:51214995-51215014 MS.gene050031:intron 40.0%
GGACATGCCTTAATCACAAA+TGG + chr5.4:51215321-51215340 None:intergenic 40.0%
GTGATGAAATGGGGATTACA+TGG - chr5.4:51214969-51214988 MS.gene050031:CDS 40.0%
TATCCTAGCATAACATGGCA+AGG - chr5.4:51215962-51215981 MS.gene050031:intron 40.0%
TGACCATATGTCAGCTGATA+TGG - chr5.4:51216101-51216120 MS.gene050031:intron 40.0%
TGAGAAATTGACCTTACCTC+AGG + chr5.4:51215495-51215514 None:intergenic 40.0%
TGCACCTGATGATCAAATTC+CGG + chr5.4:51216360-51216379 None:intergenic 40.0%
TTGTTTACTGCTTGGGATTG+TGG - chr5.4:51215071-51215090 MS.gene050031:intron 40.0%
! ACAAACACAGAAAGTACACG+AGG + chr5.4:51216174-51216193 None:intergenic 40.0%
! AGGCTTTGTGCTTTCTTTCA+CGG + chr5.4:51216295-51216314 None:intergenic 40.0%
! CATGATCAGTTGAGAAGTAC+TGG + chr5.4:51215755-51215774 None:intergenic 40.0%
! GAAAGTGATGCATTTGGTGA+AGG - chr5.4:51214869-51214888 MS.gene050031:intron 40.0%
! GAGCAATCTGAAGTTGTTTC+TGG - chr5.4:51215026-51215045 MS.gene050031:intron 40.0%
! GCTAATGGACAAGCATTAGT+AGG + chr5.4:51215635-51215654 None:intergenic 40.0%
! TGCATCACTTTCCCATTTTC+AGG + chr5.4:51214861-51214880 None:intergenic 40.0%
! TTACATGATGGGACAATCAG+AGG + chr5.4:51215657-51215676 None:intergenic 40.0%
!! ACGAGGTATTGTGAGCAATA+AGG + chr5.4:51216157-51216176 None:intergenic 40.0%
ATGGGACAATCAGAGGCTAA+TGG + chr5.4:51215650-51215669 None:intergenic 45.0%
CTACTGATGAACCTGAGGTA+AGG - chr5.4:51215481-51215500 MS.gene050031:intron 45.0%
GCACCTGATGATCAAATTCC+GGG + chr5.4:51216359-51216378 None:intergenic 45.0%
TCACATCACATGTGTCGTGT+CGG + chr5.4:51215349-51215368 None:intergenic 45.0%
TCAGGTTCATCAGTAGTCCA+TGG + chr5.4:51215477-51215496 None:intergenic 45.0%
TCTCCTTGCCATGTTATGCT+AGG + chr5.4:51215968-51215987 None:intergenic 45.0%
TGCTCATCTTCATCCTCAAC+AGG + chr5.4:51215011-51215030 None:intergenic 45.0%
TTTGTGCTTTCTTTCACGGG+AGG + chr5.4:51216291-51216310 None:intergenic 45.0%
! ATGGACTACTGATGAACCTG+AGG - chr5.4:51215476-51215495 MS.gene050031:intron 45.0%
! GGCTTTGTGCTTTCTTTCAC+GGG + chr5.4:51216294-51216313 None:intergenic 45.0%
! TTTTGTGCAGTGTGTGTAGC+AGG - chr5.4:51215226-51215245 MS.gene050031:intron 45.0%
ACTGCTTGGGATTGTGGAAG+TGG - chr5.4:51215077-51215096 MS.gene050031:intron 50.0%
GGGATTGTGGAAGTGGAAGA+AGG - chr5.4:51215084-51215103 MS.gene050031:intron 50.0%
GTGTGTAGCAGGAACTCTTC+TGG - chr5.4:51215237-51215256 MS.gene050031:intron 50.0%
TCGCCCGGAATTTGATCATC+AGG - chr5.4:51216353-51216372 MS.gene050031:intron 50.0%
TGTAGCAGGAACTCTTCTGG+TGG - chr5.4:51215240-51215259 MS.gene050031:intron 50.0%
TTTGAGGGCAATTGATCGCC+CGG - chr5.4:51216338-51216357 MS.gene050031:intron 50.0%
ACATGTGTCGTGTCGGACAC+CGG + chr5.4:51215342-51215361 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 51214834 51216633 51214834 ID=MS.gene050031
chr5.4 mRNA 51214834 51216633 51214834 ID=MS.gene050031.t1;Parent=MS.gene050031
chr5.4 exon 51216362 51216633 51216362 ID=MS.gene050031.t1.exon1;Parent=MS.gene050031.t1
chr5.4 CDS 51216362 51216633 51216362 ID=cds.MS.gene050031.t1;Parent=MS.gene050031.t1
chr5.4 exon 51216186 51216231 51216186 ID=MS.gene050031.t1.exon2;Parent=MS.gene050031.t1
chr5.4 CDS 51216186 51216231 51216186 ID=cds.MS.gene050031.t1;Parent=MS.gene050031.t1
chr5.4 exon 51215093 51215191 51215093 ID=MS.gene050031.t1.exon3;Parent=MS.gene050031.t1
chr5.4 CDS 51215093 51215191 51215093 ID=cds.MS.gene050031.t1;Parent=MS.gene050031.t1
chr5.4 exon 51214951 51215010 51214951 ID=MS.gene050031.t1.exon4;Parent=MS.gene050031.t1
chr5.4 CDS 51214951 51215010 51214951 ID=cds.MS.gene050031.t1;Parent=MS.gene050031.t1
chr5.4 exon 51214834 51214845 51214834 ID=MS.gene050031.t1.exon5;Parent=MS.gene050031.t1
chr5.4 CDS 51214834 51214845 51214834 ID=cds.MS.gene050031.t1;Parent=MS.gene050031.t1
Gene Sequence

>MS.gene050031

ATGGAAGAAGCAAGGAATAGTATACCTGAAAATGGGAAAGTGATGCATTTGGTGAAGGTTTTTGAGAGGTTACTTAGTATAAAAAGGGAGAAAGAGAAGAATGAGGATGAGGAAGAGAATGAGAAGAAGAATAAGGTGATGAAATGGGGATTACATGGATTGCAATTTCAACAACCTGTTGAGGATGAAGATGAGCAATCTGAAGTTGTTTCTGGTTGTCGTGATGATGATGCTAGTTTGTTTACTGCTTGGGATTGTGGAAGTGGAAGAAGGTTTTTGTGTTTTACTTCTTTTACTTTGATAACTCTAAAGTTTGTTTCATTTTATTTGAATTTGATTTGATTCATTTTGCTTGAGATTGTTGACTTGTGTCAATTGTTGTGTGCGCTTTTTTTTGTGCAGTGTGTGTAGCAGGAACTCTTCTGGTGGTAGAAGAAGCAGAAGAAATGTAAGATTTTTAGATGATGTTATTGTTATTGTATTTCCATTTGTGATTAAGGCATGTCCGGTGTCCGACACGACACATGTGATGTGATTTATGTCATTTCTGCAAAATGTTATCGGTATCAACGTGTTAGTGACATGCGGTTACTATAATACTAATGAAGATTGGTTAGTGATTCACTATTAACAAGGTCTTCCATGGACTACTGATGAACCTGAGGTAAGGTCAATTTCTCATGTCAATCCTTAGTTTGAAGTGTATATGCTTAGTAACAACTCACAGTCAAATTTGGTCTATTTTTGCTCTTGCTAAGTATAAATGTAATTTGTTAATCAAGCATAGGCTCGTTTATGCCTACTAATGCTTGTCCATTAGCCTCTGATTGTCCCATCATGTAATGCAATTACGATTATAGCTTATGTCTGGTGGTTCAGTATAAATTTTCACGCACTGTCAGGGTAAAAAAAATTACACCAGTACTTCTCAACTGATCATGTGTATGACTCTGGAAGTAGTTATAATAAAGTCAAAACTTTCAGTTTATTTGATGGTTATGATTAATTGACAGTTTAAAAATTCTTTACATTGACGGTGTATATGAATTAAATCTGTGAGTAGTTGGTTATATGATCTTGCTTCAAAACTTTGAACTGATTTATATTATAAAGCAGCCTTATATCTAATATCCTAGCATAACATGGCAAGGAGATAGCAATTCTCAATTAGAACGGTACATTGTGGCAAGCATTCTATGTCATCTCATATTTAGCTACCCTTAATGATGTTGCTGCGTTAATCTCCAATAAACATCCTCTTGAGTCTTGACCATATGTCAGCTGATATGGAGTTAATTCAGTTTCAGTGGAATTAGAAATCCTTATTGCTCACAATACCTCGTGTACTTTCTGTGTTTGTAATCAAAGTTTTTCTTTGTCAAATTTATCATAATTCTGTTGGAAGTCTCTACTGCTTGTCCATTAGCATTCTTCTGTTACAGTGCTTGCTAAAACCTCCCGTGAAAGAAAGCACAAAGCCTATTGATGTGAAGCTTCATAGTGATTTGAGGGCAATTGATCGCCCGGAATTTGATCATCAGGTGCATATTTCATCTACTCTTTCTTTCCCCTTTAGTTTTACACATCATGAAATGTAAAATTGATGAATGCTTAACTGTGTAGGTCGCTGAAAAATTGAGTTTGATAGAACAACAGAAAAGGGAATTGGAAATACAACAAAAGGTACCATGTTTACCAACTTTCACATCTCAAATCAGTGTGTCCTAATTTTGAGTTGCCATGTTTTCCTTTATCTAAAAGTTGACGTCGGTTTATATTCCCTTACAGTTGGCTGAATAG

Protein sequence

>MS.gene050031.t1

MEEARNSIPENGKVMHLVKVFERLLSIKREKEKNEDEEENEKKNKVMKWGLHGLQFQQPVEDEDEQSEVVSGCRDDDASLFTAWDCGSGRSVCSRNSSGGRRSRRNCLLKPPVKESTKPIDVKLHSDLRAIDRPEFDHQVAEKLSLIEQQKRELEIQQKLAE