Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07394.t1 | XP_003595099.1 | 98 | 102 | 2 | 0 | 39 | 140 | 143 | 244 | 7.50E-51 | 209.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07394.t1 | P60039 | 85.3 | 102 | 15 | 0 | 39 | 140 | 141 | 242 | 1.3e-48 | 193.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07394.t1 | A0A072VPV6 | 98.0 | 102 | 2 | 0 | 39 | 140 | 174 | 275 | 5.4e-51 | 209.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene07394 | 0.805057 | 1.60E-49 | -1.69E-46 |
| MS.gene049203 | MS.gene07394 | 0.806271 | 8.92E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene07394 | 0.860627 | 1.81E-63 | -1.69E-46 |
| MS.gene049343 | MS.gene07394 | 0.837851 | 4.07E-57 | -1.69E-46 |
| MS.gene049350 | MS.gene07394 | 0.825069 | 5.73E-54 | -1.69E-46 |
| MS.gene049404 | MS.gene07394 | 0.814426 | 1.55E-51 | -1.69E-46 |
| MS.gene049666 | MS.gene07394 | 0.8088 | 2.59E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene07394 | 0.839367 | 1.65E-57 | -1.69E-46 |
| MS.gene049821 | MS.gene07394 | 0.803945 | 2.74E-49 | -1.69E-46 |
| MS.gene049824 | MS.gene07394 | 0.8198 | 9.60E-53 | -1.69E-46 |
| MS.gene049825 | MS.gene07394 | 0.852616 | 4.11E-61 | -1.69E-46 |
| MS.gene049850 | MS.gene07394 | 0.83064 | 2.62E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene07394 | 0.860158 | 2.51E-63 | -1.69E-46 |
| MS.gene049977 | MS.gene07394 | 0.809422 | 1.91E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene07394 | 0.848201 | 7.13E-60 | -1.69E-46 |
| MS.gene050029 | MS.gene07394 | 0.808013 | 3.82E-50 | -1.69E-46 |
| MS.gene050031 | MS.gene07394 | 0.811145 | 8.10E-51 | -1.69E-46 |
| MS.gene050043 | MS.gene07394 | 0.806572 | 7.71E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene07394 | 0.82328 | 1.51E-53 | -1.69E-46 |
| MS.gene050201 | MS.gene07394 | 0.810319 | 1.22E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene07394 | 0.815845 | 7.50E-52 | -1.69E-46 |
| MS.gene050559 | MS.gene07394 | 0.825483 | 4.57E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene07394 | 0.821194 | 4.60E-53 | -1.69E-46 |
| MS.gene050562 | MS.gene07394 | 0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene050799 | MS.gene07394 | 0.865865 | 4.33E-65 | -1.69E-46 |
| MS.gene051181 | MS.gene07394 | 0.80538 | 1.37E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene07394 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene07394 | 0.83042 | 2.97E-55 | -1.69E-46 |
| MS.gene051279 | MS.gene07394 | 0.835005 | 2.16E-56 | -1.69E-46 |
| MS.gene051594 | MS.gene07394 | 0.846355 | 2.29E-59 | -1.69E-46 |
| MS.gene051700 | MS.gene07394 | 0.802335 | 5.89E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene07394 | 0.849313 | 3.50E-60 | -1.69E-46 |
| MS.gene051720 | MS.gene07394 | 0.839755 | 1.31E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene07394 | 0.864732 | 9.84E-65 | -1.69E-46 |
| MS.gene051957 | MS.gene07394 | 0.823913 | 1.07E-53 | -1.69E-46 |
| MS.gene052208 | MS.gene07394 | 0.819639 | 1.04E-52 | -1.69E-46 |
| MS.gene052358 | MS.gene07394 | -0.816832 | 4.51E-52 | -1.69E-46 |
| MS.gene052447 | MS.gene07394 | 0.806729 | 7.14E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene07394 | 0.818238 | 2.18E-52 | -1.69E-46 |
| MS.gene052488 | MS.gene07394 | 0.806057 | 9.90E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene07394 | 0.874466 | 6.61E-68 | -1.69E-46 |
| MS.gene052561 | MS.gene07394 | 0.801761 | 7.73E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene07394 | 0.844636 | 6.69E-59 | -1.69E-46 |
| MS.gene052584 | MS.gene07394 | 0.869611 | 2.72E-66 | -1.69E-46 |
| MS.gene052685 | MS.gene07394 | 0.808354 | 3.23E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene07394 | 0.835197 | 1.93E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene07394 | 0.812296 | 4.55E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene07394 | 0.823853 | 1.11E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene07394 | 0.802425 | 5.65E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene07394 | -0.806355 | 8.56E-50 | -1.69E-46 |
| MS.gene053084 | MS.gene07394 | 0.800854 | 1.19E-48 | -1.69E-46 |
| MS.gene053122 | MS.gene07394 | 0.800319 | 1.52E-48 | -1.69E-46 |
| MS.gene053144 | MS.gene07394 | 0.802978 | 4.34E-49 | -1.69E-46 |
| MS.gene053145 | MS.gene07394 | 0.841481 | 4.63E-58 | -1.69E-46 |
| MS.gene053221 | MS.gene07394 | 0.815392 | 9.46E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene07394 | 0.864244 | 1.40E-64 | -1.69E-46 |
| MS.gene053396 | MS.gene07394 | 0.822601 | 2.17E-53 | -1.69E-46 |
| MS.gene053592 | MS.gene07394 | 0.804466 | 2.13E-49 | -1.69E-46 |
| MS.gene053599 | MS.gene07394 | 0.802651 | 5.07E-49 | -1.69E-46 |
| MS.gene05365 | MS.gene07394 | 0.83258 | 8.72E-56 | -1.69E-46 |
| MS.gene053883 | MS.gene07394 | 0.819519 | 1.11E-52 | -1.69E-46 |
| MS.gene053900 | MS.gene07394 | 0.833957 | 3.96E-56 | -1.69E-46 |
| MS.gene053966 | MS.gene07394 | 0.815181 | 1.05E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene07394 | 0.827979 | 1.16E-54 | -1.69E-46 |
| MS.gene053998 | MS.gene07394 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene07394 | 0.818631 | 1.77E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene07394 | 0.837228 | 5.88E-57 | -1.69E-46 |
| MS.gene05422 | MS.gene07394 | 0.816961 | 4.22E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene07394 | 0.810839 | 9.44E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene07394 | 0.864996 | 8.13E-65 | -1.69E-46 |
| MS.gene054291 | MS.gene07394 | -0.827186 | 1.80E-54 | -1.69E-46 |
| MS.gene054576 | MS.gene07394 | 0.822476 | 2.32E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene07394 | 0.802976 | 4.34E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene07394 | 0.860473 | 2.02E-63 | -1.69E-46 |
| MS.gene054775 | MS.gene07394 | 0.836448 | 9.30E-57 | -1.69E-46 |
| MS.gene054967 | MS.gene07394 | 0.81312 | 3.01E-51 | -1.69E-46 |
| MS.gene054991 | MS.gene07394 | 0.838974 | 2.09E-57 | -1.69E-46 |
| MS.gene055228 | MS.gene07394 | 0.820205 | 7.76E-53 | -1.69E-46 |
| MS.gene055374 | MS.gene07394 | 0.81372 | 2.22E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene07394 | 0.808448 | 3.08E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene07394 | 0.828797 | 7.36E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene07394 | 0.807177 | 5.74E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene07394 | -0.814283 | 1.67E-51 | -1.69E-46 |
| MS.gene055586 | MS.gene07394 | 0.820748 | 5.82E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene07394 | 0.841812 | 3.79E-58 | -1.69E-46 |
| MS.gene055694 | MS.gene07394 | 0.82553 | 4.46E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene07394 | 0.82712 | 1.86E-54 | -1.69E-46 |
| MS.gene055808 | MS.gene07394 | 0.800351 | 1.50E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene07394 | 0.849692 | 2.75E-60 | -1.69E-46 |
| MS.gene056050 | MS.gene07394 | 0.847717 | 9.69E-60 | -1.69E-46 |
| MS.gene056091 | MS.gene07394 | 0.813536 | 2.44E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene07394 | 0.809244 | 2.08E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene07394 | 0.830603 | 2.68E-55 | -1.69E-46 |
| MS.gene056272 | MS.gene07394 | 0.829999 | 3.76E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene07394 | 0.802848 | 4.62E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene07394 | 0.847876 | 8.76E-60 | -1.69E-46 |
| MS.gene056373 | MS.gene07394 | 0.816747 | 4.71E-52 | -1.69E-46 |
| MS.gene056498 | MS.gene07394 | 0.808249 | 3.40E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene07394 | 0.82779 | 1.29E-54 | -1.69E-46 |
| MS.gene056854 | MS.gene07394 | 0.803431 | 3.50E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene07394 | 0.827705 | 1.35E-54 | -1.69E-46 |
| MS.gene05690 | MS.gene07394 | 0.819726 | 9.98E-53 | -1.69E-46 |
| MS.gene057021 | MS.gene07394 | 0.805549 | 1.26E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene07394 | 0.814819 | 1.27E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene07394 | 0.824353 | 8.45E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene07394 | 0.815768 | 7.80E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene07394 | 0.817751 | 2.80E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene07394 | 0.837003 | 6.72E-57 | -1.69E-46 |
| MS.gene057314 | MS.gene07394 | 0.800758 | 1.24E-48 | -1.69E-46 |
| MS.gene057414 | MS.gene07394 | 0.828439 | 8.99E-55 | -1.69E-46 |
| MS.gene057444 | MS.gene07394 | 0.841081 | 5.90E-58 | -1.69E-46 |
| MS.gene057598 | MS.gene07394 | 0.823748 | 1.17E-53 | -1.69E-46 |
| MS.gene057915 | MS.gene07394 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene057921 | MS.gene07394 | 0.816272 | 6.03E-52 | -1.69E-46 |
| MS.gene058008 | MS.gene07394 | 0.819901 | 9.11E-53 | -1.69E-46 |
| MS.gene058009 | MS.gene07394 | 0.822628 | 2.14E-53 | -1.69E-46 |
| MS.gene058037 | MS.gene07394 | 0.855495 | 6.07E-62 | -1.69E-46 |
| MS.gene058279 | MS.gene07394 | 0.804048 | 2.60E-49 | -1.69E-46 |
| MS.gene058294 | MS.gene07394 | 0.809348 | 1.98E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene07394 | 0.869978 | 2.06E-66 | -1.69E-46 |
| MS.gene058636 | MS.gene07394 | 0.809257 | 2.07E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene07394 | 0.800873 | 1.18E-48 | -1.69E-46 |
| MS.gene058829 | MS.gene07394 | 0.826232 | 3.04E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene07394 | 0.819063 | 1.41E-52 | -1.69E-46 |
| MS.gene058984 | MS.gene07394 | 0.802019 | 6.84E-49 | -1.69E-46 |
| MS.gene059015 | MS.gene07394 | 0.828269 | 9.88E-55 | -1.69E-46 |
| MS.gene059084 | MS.gene07394 | 0.815756 | 7.85E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene07394 | 0.825851 | 3.74E-54 | -1.69E-46 |
| MS.gene059458 | MS.gene07394 | 0.801161 | 1.03E-48 | -1.69E-46 |
| MS.gene059459 | MS.gene07394 | 0.811122 | 8.20E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene07394 | 0.814044 | 1.88E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene07394 | 0.834525 | 2.85E-56 | -1.69E-46 |
| MS.gene059584 | MS.gene07394 | 0.816095 | 6.60E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene07394 | 0.871196 | 8.22E-67 | -1.69E-46 |
| MS.gene05985 | MS.gene07394 | -0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene060348 | MS.gene07394 | 0.801141 | 1.04E-48 | -1.69E-46 |
| MS.gene060352 | MS.gene07394 | 0.832239 | 1.06E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene07394 | 0.821566 | 3.77E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene07394 | 0.820301 | 7.37E-53 | -1.69E-46 |
| MS.gene060519 | MS.gene07394 | 0.811239 | 7.73E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene07394 | 0.837743 | 4.34E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene07394 | 0.81678 | 4.64E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene07394 | 0.815852 | 7.47E-52 | -1.69E-46 |
| MS.gene060634 | MS.gene07394 | -0.837296 | 5.65E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene07394 | 0.801892 | 7.27E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07394.t1 | MTR_2g038250 | 98.039 | 102 | 2 | 0 | 39 | 140 | 143 | 244 | 9.26e-71 | 213 |
| MS.gene07394.t1 | MTR_1g022385 | 98.039 | 102 | 2 | 0 | 39 | 140 | 174 | 275 | 2.07e-70 | 213 |
| MS.gene07394.t1 | MTR_4g008160 | 89.216 | 102 | 11 | 0 | 39 | 140 | 142 | 243 | 3.38e-65 | 198 |
| MS.gene07394.t1 | MTR_1g022375 | 76.829 | 82 | 19 | 0 | 49 | 130 | 75 | 156 | 3.26e-42 | 137 |
| MS.gene07394.t1 | MTR_7g059170 | 54.902 | 102 | 46 | 0 | 39 | 140 | 145 | 246 | 1.49e-40 | 135 |
| MS.gene07394.t1 | MTR_1g108785 | 75.676 | 37 | 8 | 1 | 97 | 132 | 23 | 59 | 1.68e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07394.t1 | AT2G44120 | 85.294 | 102 | 15 | 0 | 39 | 140 | 141 | 242 | 3.22e-64 | 196 |
| MS.gene07394.t1 | AT2G44120 | 85.294 | 102 | 15 | 0 | 39 | 140 | 146 | 247 | 4.18e-64 | 196 |
| MS.gene07394.t1 | AT2G01250 | 86.275 | 102 | 14 | 0 | 39 | 140 | 141 | 242 | 6.90e-64 | 195 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT3G13580 | 84.314 | 102 | 16 | 0 | 39 | 140 | 143 | 244 | 5.89e-63 | 193 |
| MS.gene07394.t1 | AT1G80750 | 52.941 | 102 | 47 | 1 | 40 | 140 | 146 | 247 | 1.13e-30 | 110 |
| MS.gene07394.t1 | AT2G01250 | 72.973 | 37 | 10 | 0 | 39 | 75 | 141 | 177 | 4.97e-11 | 58.2 |
Find 37 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCATTAGAAGGATGAATT+AGG | 0.270598 | 1.2:+64334757 | MS.gene07394:CDS |
| TTTCAAGCTCAAAGCTCCTT+TGG | 0.284641 | 1.2:+64334661 | MS.gene07394:CDS |
| AGTGTTCAATTGCAGGTTCT+TGG | 0.317764 | 1.2:+64334548 | MS.gene07394:intron |
| TTCAAGCTCAAAGCTCCTTT+GGG | 0.329811 | 1.2:+64334662 | MS.gene07394:CDS |
| TTGATCTACAAGCGCGGTTA+TGG | 0.337108 | 1.2:+64334199 | MS.gene07394:CDS |
| TCAATTCTCTAACACTCTTC+AGG | 0.373375 | 1.2:-64334180 | None:intergenic |
| AAGGTGGTGATGCTGGTAAC+AGG | 0.401808 | 1.2:+64334717 | MS.gene07394:CDS |
| GTGTTCAATTGCAGGTTCTT+GGG | 0.403327 | 1.2:+64334549 | MS.gene07394:intron |
| ACATCAATGAACTCATTAGA+AGG | 0.462677 | 1.2:+64334747 | MS.gene07394:CDS |
| GAAGCGTGAAGCTAAGCTTA+AGG | 0.464202 | 1.2:+64334140 | MS.gene07394:intron |
| AAGCGTGAAGCTAAGCTTAA+GGG | 0.467088 | 1.2:+64334141 | MS.gene07394:intron |
| AGATAACTCTATCATTGAAC+AGG | 0.482014 | 1.2:+64334252 | MS.gene07394:CDS |
| TTTATGCATCTACGGAGGTC+AGG | 0.484893 | 1.2:+64333759 | None:intergenic |
| AAGAGAAATCACTATGTTGA+AGG | 0.489111 | 1.2:+64334698 | MS.gene07394:CDS |
| AAAGGAGCTTTGAGCTTGAA+AGG | 0.504831 | 1.2:-64334659 | None:intergenic |
| GAGAGGCAAACAACTTCCTA+TGG | 0.504947 | 1.2:+64334636 | MS.gene07394:CDS |
| TATGTTGAAGGTGGTGATGC+TGG | 0.533299 | 1.2:+64334710 | MS.gene07394:CDS |
| AAGCTGAGAAAGAACATGGT+TGG | 0.534698 | 1.2:+64333814 | MS.gene07394:CDS |
| TTGCAGGTTCTTGGGAAACA+TGG | 0.547799 | 1.2:+64334557 | MS.gene07394:intron |
| ATCCACGAGATCATGACTGT+TGG | 0.549865 | 1.2:+64334602 | MS.gene07394:CDS |
| GCGGTTATGGAAAGGTTGAT+AGG | 0.549876 | 1.2:+64334212 | MS.gene07394:CDS |
| TTCTTCTTCATGCCACCCAA+AGG | 0.554983 | 1.2:-64334677 | None:intergenic |
| TGACAGGGTTTATGCATCTA+CGG | 0.562090 | 1.2:+64333751 | None:intergenic |
| CTTTGAGCTTGAAAGGCCAT+AGG | 0.568033 | 1.2:-64334652 | None:intergenic |
| AAGCTCAAAGCTCCTTTGGG+TGG | 0.570830 | 1.2:+64334665 | MS.gene07394:CDS |
| ATCCAACAGTCATGATCTCG+TGG | 0.585385 | 1.2:-64334604 | None:intergenic |
| CTACAAGCGCGGTTATGGAA+AGG | 0.599869 | 1.2:+64334204 | MS.gene07394:CDS |
| CGTGAAGCTAAGCTTAAGGG+TGG | 0.634387 | 1.2:+64334144 | MS.gene07394:CDS |
| GAGAAAGCTGAGAAAGAACA+TGG | 0.636998 | 1.2:+64333810 | MS.gene07394:CDS |
| AGAAATCACTATGTTGAAGG+TGG | 0.637998 | 1.2:+64334701 | MS.gene07394:CDS |
| GAGGTCAGGAGAGGCTACAG+AGG | 0.640976 | 1.2:+64333773 | MS.gene07394:CDS |
| AGGTGGTGATGCTGGTAACA+GGG | 0.651466 | 1.2:+64334718 | MS.gene07394:CDS |
| GCATCTACGGAGGTCAGGAG+AGG | 0.664972 | 1.2:+64333764 | MS.gene07394:CDS |
| AGAGAATTGATCTACAAGCG+CGG | 0.721136 | 1.2:+64334193 | MS.gene07394:CDS |
| TGTTGGATCTCACTTCAGAG+AGG | 0.724978 | 1.2:+64334619 | MS.gene07394:CDS |
| TGGTGATGCTGGTAACAGGG+AGG | 0.745630 | 1.2:+64334721 | MS.gene07394:CDS |
| CAGGGTTTATGCATCTACGG+AGG | 0.767701 | 1.2:+64333754 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAATAGAAAAATTTAAC+CGG | - | chr1.2:64334519-64334538 | None:intergenic | 15.0% |
| !!! | AATGTTTTAGAATTTTAGAA+TGG | + | chr1.2:64334078-64334097 | MS.gene07394:intron | 15.0% |
| !!! | TTCTTTCTTTGACTTATATA+TGG | + | chr1.2:64333928-64333947 | MS.gene07394:intron | 20.0% |
| !!! | TTTAGAATTTTAGAATGGTA+AGG | + | chr1.2:64334083-64334102 | MS.gene07394:intron | 20.0% |
| ! | CTATCATATTTGTTCTTGTA+GGG | + | chr1.2:64333963-64333982 | MS.gene07394:intron | 25.0% |
| ! | GTTTATCCAATTAATAGTGA+GGG | + | chr1.2:64334035-64334054 | MS.gene07394:intron | 25.0% |
| ! | TGTTTATCCAATTAATAGTG+AGG | + | chr1.2:64334034-64334053 | MS.gene07394:intron | 25.0% |
| !! | AATTTAAAGTACGTACTTGT+GGG | + | chr1.2:64333995-64334014 | MS.gene07394:intron | 25.0% |
| !! | TAATTTAAAGTACGTACTTG+TGG | + | chr1.2:64333994-64334013 | MS.gene07394:intron | 25.0% |
| AAGAGAAATCACTATGTTGA+AGG | + | chr1.2:64334698-64334717 | MS.gene07394:CDS | 30.0% | |
| ACATCAATGAACTCATTAGA+AGG | + | chr1.2:64334747-64334766 | MS.gene07394:CDS | 30.0% | |
| AGATAACTCTATCATTGAAC+AGG | + | chr1.2:64334252-64334271 | MS.gene07394:CDS | 30.0% | |
| AGTCAAAGAAAGAATCTCAT+TGG | - | chr1.2:64333922-64333941 | None:intergenic | 30.0% | |
| GCTATCATATTTGTTCTTGT+AGG | + | chr1.2:64333962-64333981 | MS.gene07394:intron | 30.0% | |
| !! | GGTGCTATTATTATTGTTGA+AGG | + | chr1.2:64334485-64334504 | MS.gene07394:intron | 30.0% |
| AACAACTCACATTCTGACTT+TGG | - | chr1.2:64334390-64334409 | None:intergenic | 35.0% | |
| AATAATAGCACCACCATACA+GGG | - | chr1.2:64334477-64334496 | None:intergenic | 35.0% | |
| ACATTCTGACTTTGGCAAAT+TGG | - | chr1.2:64334382-64334401 | None:intergenic | 35.0% | |
| AGAAATCACTATGTTGAAGG+TGG | + | chr1.2:64334701-64334720 | MS.gene07394:CDS | 35.0% | |
| ATCCAATTAATAGTGAGGGA+TGG | + | chr1.2:64334039-64334058 | MS.gene07394:intron | 35.0% | |
| ATTGTTGAAGGTTATGTTGC+CGG | + | chr1.2:64334497-64334516 | MS.gene07394:intron | 35.0% | |
| CATTCTGACTTTGGCAAATT+GGG | - | chr1.2:64334381-64334400 | None:intergenic | 35.0% | |
| CTGAACTGTATGACATAGAT+GGG | + | chr1.2:64334326-64334345 | MS.gene07394:intron | 35.0% | |
| GTGATAACTACATCCAATGA+GGG | - | chr1.2:64333895-64333914 | None:intergenic | 35.0% | |
| TAATAATAGCACCACCATAC+AGG | - | chr1.2:64334478-64334497 | None:intergenic | 35.0% | |
| TACCATCCCTCACTATTAAT+TGG | - | chr1.2:64334044-64334063 | None:intergenic | 35.0% | |
| TCAATTCTCTAACACTCTTC+AGG | - | chr1.2:64334183-64334202 | None:intergenic | 35.0% | |
| TCTGAACTGTATGACATAGA+TGG | + | chr1.2:64334325-64334344 | MS.gene07394:intron | 35.0% | |
| TGTGATAACTACATCCAATG+AGG | - | chr1.2:64333896-64333915 | None:intergenic | 35.0% | |
| !!! | CTTTGAAACATCCTTTTCCA+GGG | + | chr1.2:64333866-64333885 | MS.gene07394:intron | 35.0% |
| !!! | TCTTTGAAACATCCTTTTCC+AGG | + | chr1.2:64333865-64333884 | MS.gene07394:intron | 35.0% |
| AAGCGTGAAGCTAAGCTTAA+GGG | + | chr1.2:64334141-64334160 | MS.gene07394:intron | 40.0% | |
| AAGCTGAGAAAGAACATGGT+TGG | + | chr1.2:64333814-64333833 | MS.gene07394:CDS | 40.0% | |
| AGAGAATTGATCTACAAGCG+CGG | + | chr1.2:64334193-64334212 | MS.gene07394:CDS | 40.0% | |
| AGTGTTCAATTGCAGGTTCT+TGG | + | chr1.2:64334548-64334567 | MS.gene07394:intron | 40.0% | |
| ATAATAGCACCACCATACAG+GGG | - | chr1.2:64334476-64334495 | None:intergenic | 40.0% | |
| CTGACATAGTGTTCAATTGC+AGG | + | chr1.2:64334541-64334560 | MS.gene07394:intron | 40.0% | |
| GAGAAAGCTGAGAAAGAACA+TGG | + | chr1.2:64333810-64333829 | MS.gene07394:CDS | 40.0% | |
| GGAATACGATGATTCGCAAA+AGG | + | chr1.2:64334104-64334123 | MS.gene07394:intron | 40.0% | |
| GTGTTCAATTGCAGGTTCTT+GGG | + | chr1.2:64334549-64334568 | MS.gene07394:intron | 40.0% | |
| TGTGTGATCGAATGAAAGCA+GGG | - | chr1.2:64334297-64334316 | None:intergenic | 40.0% | |
| TTCAAGCTCAAAGCTCCTTT+GGG | + | chr1.2:64334662-64334681 | MS.gene07394:CDS | 40.0% | |
| TTTCAAGCTCAAAGCTCCTT+TGG | + | chr1.2:64334661-64334680 | MS.gene07394:CDS | 40.0% | |
| !! | AAAGGAGCTTTGAGCTTGAA+AGG | - | chr1.2:64334662-64334681 | None:intergenic | 40.0% |
| AATGAGGGATTCCCTGGAAA+AGG | - | chr1.2:64333880-64333899 | None:intergenic | 45.0% | |
| ATCCAACAGTCATGATCTCG+TGG | - | chr1.2:64334607-64334626 | None:intergenic | 45.0% | |
| ATCCACGAGATCATGACTGT+TGG | + | chr1.2:64334602-64334621 | MS.gene07394:CDS | 45.0% | |
| CTGTGTGATCGAATGAAAGC+AGG | - | chr1.2:64334298-64334317 | None:intergenic | 45.0% | |
| CTTTGAGCTTGAAAGGCCAT+AGG | - | chr1.2:64334655-64334674 | None:intergenic | 45.0% | |
| GAAGCGTGAAGCTAAGCTTA+AGG | + | chr1.2:64334140-64334159 | MS.gene07394:intron | 45.0% | |
| GAGAGGCAAACAACTTCCTA+TGG | + | chr1.2:64334636-64334655 | MS.gene07394:CDS | 45.0% | |
| TGTTGGATCTCACTTCAGAG+AGG | + | chr1.2:64334619-64334638 | MS.gene07394:CDS | 45.0% | |
| TTCTTCTTCATGCCACCCAA+AGG | - | chr1.2:64334680-64334699 | None:intergenic | 45.0% | |
| TTGATCTACAAGCGCGGTTA+TGG | + | chr1.2:64334199-64334218 | MS.gene07394:CDS | 45.0% | |
| TTGCAGGTTCTTGGGAAACA+TGG | + | chr1.2:64334557-64334576 | MS.gene07394:intron | 45.0% | |
| ! | GCGGTTATGGAAAGGTTGAT+AGG | + | chr1.2:64334212-64334231 | MS.gene07394:CDS | 45.0% |
| ! | TATGTTGAAGGTGGTGATGC+TGG | + | chr1.2:64334710-64334729 | MS.gene07394:CDS | 45.0% |
| ! | TTTTCCAGGGAATCCCTCAT+TGG | + | chr1.2:64333879-64333898 | MS.gene07394:intron | 45.0% |
| AAGCTCAAAGCTCCTTTGGG+TGG | + | chr1.2:64334665-64334684 | MS.gene07394:CDS | 50.0% | |
| ACATCCAATGAGGGATTCCC+TGG | - | chr1.2:64333886-64333905 | None:intergenic | 50.0% | |
| CGTGAAGCTAAGCTTAAGGG+TGG | + | chr1.2:64334144-64334163 | MS.gene07394:CDS | 50.0% | |
| CTACAAGCGCGGTTATGGAA+AGG | + | chr1.2:64334204-64334223 | MS.gene07394:CDS | 50.0% | |
| CTTGACCAATGTCCCCTGTA+TGG | + | chr1.2:64334461-64334480 | MS.gene07394:intron | 50.0% | |
| ! | AAGGTGGTGATGCTGGTAAC+AGG | + | chr1.2:64334717-64334736 | MS.gene07394:CDS | 50.0% |
| ! | AGGTGGTGATGCTGGTAACA+GGG | + | chr1.2:64334718-64334737 | MS.gene07394:CDS | 50.0% |
| CACCACCATACAGGGGACAT+TGG | - | chr1.2:64334469-64334488 | None:intergenic | 55.0% | |
| GACCAATGTCCCCTGTATGG+TGG | + | chr1.2:64334464-64334483 | MS.gene07394:intron | 55.0% | |
| ! | TGGTGATGCTGGTAACAGGG+AGG | + | chr1.2:64334721-64334740 | MS.gene07394:CDS | 55.0% |
| GAGGTCAGGAGAGGCTACAG+AGG | + | chr1.2:64333773-64333792 | MS.gene07394:CDS | 60.0% | |
| GCATCTACGGAGGTCAGGAG+AGG | + | chr1.2:64333764-64333783 | MS.gene07394:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 64333762 | 64334778 | 64333762 | ID=MS.gene07394 |
| chr1.2 | mRNA | 64333762 | 64334778 | 64333762 | ID=MS.gene07394.t1;Parent=MS.gene07394 |
| chr1.2 | exon | 64333762 | 64333838 | 64333762 | ID=MS.gene07394.t1.exon1;Parent=MS.gene07394.t1 |
| chr1.2 | CDS | 64333762 | 64333838 | 64333762 | ID=cds.MS.gene07394.t1;Parent=MS.gene07394.t1 |
| chr1.2 | exon | 64334144 | 64334273 | 64334144 | ID=MS.gene07394.t1.exon2;Parent=MS.gene07394.t1 |
| chr1.2 | CDS | 64334144 | 64334273 | 64334144 | ID=cds.MS.gene07394.t1;Parent=MS.gene07394.t1 |
| chr1.2 | exon | 64334563 | 64334778 | 64334563 | ID=MS.gene07394.t1.exon3;Parent=MS.gene07394.t1 |
| chr1.2 | CDS | 64334563 | 64334778 | 64334563 | ID=cds.MS.gene07394.t1;Parent=MS.gene07394.t1 |
| Gene Sequence |
| Protein sequence |
