Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067208.t1 | XP_004503599.1 | 50 | 80 | 37 | 1 | 27 | 106 | 91 | 167 | 4.70E-10 | 75.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067208.t1 | O65155 | 40.8 | 103 | 51 | 2 | 11 | 106 | 62 | 161 | 7.4e-12 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067208.t1 | A0A2P2JC59 | 43.6 | 94 | 47 | 2 | 16 | 106 | 59 | 149 | 6.8e-11 | 77.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene067208 | 0.80311 | 4.08E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene067208 | 0.861142 | 1.26E-63 | -1.69E-46 |
| MS.gene049271 | MS.gene067208 | 0.820512 | 6.60E-53 | -1.69E-46 |
| MS.gene049272 | MS.gene067208 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene049404 | MS.gene067208 | 0.814617 | 1.41E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene067208 | 0.814702 | 1.35E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene067208 | 0.803426 | 3.51E-49 | -1.69E-46 |
| MS.gene049567 | MS.gene067208 | 0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene049669 | MS.gene067208 | 0.805528 | 1.28E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene067208 | 0.81345 | 2.54E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene067208 | 0.82091 | 5.34E-53 | -1.69E-46 |
| MS.gene049825 | MS.gene067208 | 0.832516 | 9.04E-56 | -1.69E-46 |
| MS.gene049850 | MS.gene067208 | 0.80774 | 4.36E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene067208 | 0.835996 | 1.21E-56 | -1.69E-46 |
| MS.gene050026 | MS.gene067208 | 0.861601 | 9.15E-64 | -1.69E-46 |
| MS.gene050029 | MS.gene067208 | 0.805646 | 1.21E-49 | -1.69E-46 |
| MS.gene050031 | MS.gene067208 | 0.811222 | 7.80E-51 | -1.69E-46 |
| MS.gene050043 | MS.gene067208 | 0.814696 | 1.35E-51 | -1.69E-46 |
| MS.gene050156 | MS.gene067208 | 0.802222 | 6.21E-49 | -1.69E-46 |
| MS.gene050201 | MS.gene067208 | 0.818133 | 2.30E-52 | -1.69E-46 |
| MS.gene050273 | MS.gene067208 | 0.808418 | 3.13E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene067208 | 0.837984 | 3.76E-57 | -1.69E-46 |
| MS.gene050442 | MS.gene067208 | 0.802186 | 6.32E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene067208 | 0.838699 | 2.46E-57 | -1.69E-46 |
| MS.gene050561 | MS.gene067208 | 0.837855 | 4.06E-57 | -1.69E-46 |
| MS.gene050562 | MS.gene067208 | 0.82932 | 5.50E-55 | -1.69E-46 |
| MS.gene050750 | MS.gene067208 | 0.821406 | 4.11E-53 | -1.69E-46 |
| MS.gene050799 | MS.gene067208 | 0.802127 | 6.50E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene067208 | 0.800714 | 1.27E-48 | -1.69E-46 |
| MS.gene050953 | MS.gene067208 | 0.81007 | 1.38E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene067208 | 0.825927 | 3.59E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene067208 | 0.835062 | 2.09E-56 | -1.69E-46 |
| MS.gene051279 | MS.gene067208 | 0.84254 | 2.43E-58 | -1.69E-46 |
| MS.gene051332 | MS.gene067208 | 0.813944 | 1.98E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene067208 | 0.818568 | 1.83E-52 | -1.69E-46 |
| MS.gene051700 | MS.gene067208 | 0.814297 | 1.66E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene067208 | 0.841015 | 6.14E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene067208 | 0.845643 | 3.57E-59 | -1.69E-46 |
| MS.gene051744 | MS.gene067208 | 0.843028 | 1.80E-58 | -1.69E-46 |
| MS.gene051957 | MS.gene067208 | 0.821146 | 4.72E-53 | -1.69E-46 |
| MS.gene052190 | MS.gene067208 | 0.826495 | 2.63E-54 | -1.69E-46 |
| MS.gene052208 | MS.gene067208 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene067208 | -0.807348 | 5.28E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene067208 | 0.820321 | 7.30E-53 | -1.69E-46 |
| MS.gene052488 | MS.gene067208 | 0.825214 | 5.29E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene067208 | 0.862305 | 5.57E-64 | -1.69E-46 |
| MS.gene052534 | MS.gene067208 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene052582 | MS.gene067208 | 0.870498 | 1.39E-66 | -1.69E-46 |
| MS.gene052584 | MS.gene067208 | 0.871923 | 4.72E-67 | -1.69E-46 |
| MS.gene052586 | MS.gene067208 | 0.818881 | 1.55E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene067208 | 0.847584 | 1.05E-59 | -1.69E-46 |
| MS.gene052829 | MS.gene067208 | 0.807345 | 5.29E-50 | -1.69E-46 |
| MS.gene052843 | MS.gene067208 | 0.810011 | 1.42E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene067208 | 0.801954 | 7.06E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene067208 | 0.815604 | 8.49E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene067208 | 0.811564 | 6.57E-51 | -1.69E-46 |
| MS.gene053154 | MS.gene067208 | 0.803814 | 2.91E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene067208 | 0.823881 | 1.09E-53 | -1.69E-46 |
| MS.gene053221 | MS.gene067208 | 0.825093 | 5.66E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene067208 | 0.851296 | 9.73E-61 | -1.69E-46 |
| MS.gene053396 | MS.gene067208 | 0.800438 | 1.44E-48 | -1.69E-46 |
| MS.gene053599 | MS.gene067208 | 0.818461 | 1.94E-52 | -1.69E-46 |
| MS.gene05365 | MS.gene067208 | 0.815028 | 1.14E-51 | -1.69E-46 |
| MS.gene053966 | MS.gene067208 | 0.820441 | 6.85E-53 | -1.69E-46 |
| MS.gene053974 | MS.gene067208 | 0.821124 | 4.77E-53 | -1.69E-46 |
| MS.gene053995 | MS.gene067208 | 0.804546 | 2.05E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene067208 | 0.8205 | 6.64E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene067208 | 0.852803 | 3.63E-61 | -1.69E-46 |
| MS.gene054236 | MS.gene067208 | 0.818108 | 2.33E-52 | -1.69E-46 |
| MS.gene05423 | MS.gene067208 | 0.834418 | 3.03E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene067208 | -0.8513 | 9.71E-61 | -1.69E-46 |
| MS.gene054467 | MS.gene067208 | 0.812265 | 4.63E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene067208 | 0.804313 | 2.29E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene067208 | 0.81825 | 2.16E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene067208 | 0.830131 | 3.49E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene067208 | 0.861755 | 8.21E-64 | -1.69E-46 |
| MS.gene054775 | MS.gene067208 | 0.840349 | 9.17E-58 | -1.69E-46 |
| MS.gene054967 | MS.gene067208 | 0.816935 | 4.28E-52 | -1.69E-46 |
| MS.gene054991 | MS.gene067208 | 0.833409 | 5.42E-56 | -1.69E-46 |
| MS.gene055166 | MS.gene067208 | 0.810286 | 1.24E-50 | -1.69E-46 |
| MS.gene055228 | MS.gene067208 | 0.818275 | 2.13E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene067208 | 0.806943 | 6.43E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene067208 | 0.821702 | 3.51E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene067208 | 0.842543 | 2.43E-58 | -1.69E-46 |
| MS.gene055468 | MS.gene067208 | 0.816897 | 4.36E-52 | -1.69E-46 |
| MS.gene055586 | MS.gene067208 | 0.836143 | 1.11E-56 | -1.69E-46 |
| MS.gene055589 | MS.gene067208 | 0.818557 | 1.84E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene067208 | 0.842182 | 3.02E-58 | -1.69E-46 |
| MS.gene055808 | MS.gene067208 | 0.81024 | 1.27E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene067208 | 0.827682 | 1.37E-54 | -1.69E-46 |
| MS.gene055841 | MS.gene067208 | 0.814029 | 1.90E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene067208 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene067208 | 0.821372 | 4.18E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene067208 | 0.817943 | 2.54E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene067208 | 0.823302 | 1.49E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene067208 | 0.824031 | 1.01E-53 | -1.69E-46 |
| MS.gene056215 | MS.gene067208 | 0.822362 | 2.47E-53 | -1.69E-46 |
| MS.gene056272 | MS.gene067208 | 0.809408 | 1.92E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene067208 | 0.840215 | 9.94E-58 | -1.69E-46 |
| MS.gene056506 | MS.gene067208 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene067208 | 0.812555 | 4.00E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene067208 | 0.80018 | 1.63E-48 | -1.69E-46 |
| MS.gene056779 | MS.gene067208 | 0.803313 | 3.70E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene067208 | 0.809798 | 1.58E-50 | -1.69E-46 |
| MS.gene05690 | MS.gene067208 | 0.82041 | 6.96E-53 | -1.69E-46 |
| MS.gene057021 | MS.gene067208 | 0.802703 | 4.95E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene067208 | 0.830288 | 3.20E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene067208 | 0.807903 | 4.03E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene067208 | 0.828609 | 8.17E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene067208 | 0.819771 | 9.75E-53 | -1.69E-46 |
| MS.gene057314 | MS.gene067208 | 0.814229 | 1.71E-51 | -1.69E-46 |
| MS.gene057414 | MS.gene067208 | 0.820334 | 7.25E-53 | -1.69E-46 |
| MS.gene057444 | MS.gene067208 | 0.811266 | 7.63E-51 | -1.69E-46 |
| MS.gene057598 | MS.gene067208 | 0.810476 | 1.13E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene067208 | 0.80565 | 1.21E-49 | -1.69E-46 |
| MS.gene057921 | MS.gene067208 | 0.809307 | 2.02E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene067208 | 0.809867 | 1.53E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene067208 | 0.815529 | 8.82E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene067208 | 0.84598 | 2.89E-59 | -1.69E-46 |
| MS.gene058294 | MS.gene067208 | 0.80652 | 7.90E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene067208 | 0.86029 | 2.29E-63 | -1.69E-46 |
| MS.gene058636 | MS.gene067208 | 0.818661 | 1.74E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene067208 | 0.82743 | 1.57E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene067208 | 0.839167 | 1.86E-57 | -1.69E-46 |
| MS.gene059015 | MS.gene067208 | 0.803731 | 3.03E-49 | -1.69E-46 |
| MS.gene059084 | MS.gene067208 | 0.848748 | 5.03E-60 | -1.69E-46 |
| MS.gene05925 | MS.gene067208 | 0.806725 | 7.16E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene067208 | 0.82268 | 2.08E-53 | -1.69E-46 |
| MS.gene059458 | MS.gene067208 | 0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene059459 | MS.gene067208 | 0.812056 | 5.14E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene067208 | 0.813084 | 3.06E-51 | -1.69E-46 |
| MS.gene05950 | MS.gene067208 | 0.820494 | 6.66E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene067208 | 0.854618 | 1.09E-61 | -1.69E-46 |
| MS.gene059570 | MS.gene067208 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene067208 | 0.81904 | 1.43E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene067208 | 0.873017 | 2.04E-67 | -1.69E-46 |
| MS.gene060322 | MS.gene067208 | 0.818681 | 1.73E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene067208 | 0.810319 | 1.22E-50 | -1.69E-46 |
| MS.gene060521 | MS.gene067208 | 0.829984 | 3.79E-55 | -1.69E-46 |
| MS.gene06052 | MS.gene067208 | 0.822949 | 1.80E-53 | -1.69E-46 |
| MS.gene060553 | MS.gene067208 | 0.804643 | 1.96E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene067208 | 0.846262 | 2.43E-59 | -1.69E-46 |
| MS.gene060587 | MS.gene067208 | 0.802553 | 5.31E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene067208 | -0.845873 | 3.10E-59 | -1.69E-46 |
| MS.gene060781 | MS.gene067208 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene060879 | MS.gene067208 | 0.809676 | 1.68E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene067208 | -0.826192 | 3.10E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067208.t1 | MTR_2g010790 | 42.202 | 109 | 48 | 3 | 121 | 220 | 307 | 409 | 1.08e-13 | 69.7 |
| MS.gene067208.t1 | MTR_8g099650 | 47.297 | 74 | 36 | 1 | 29 | 102 | 91 | 161 | 2.09e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067208.t1 | AT4G10920 | 47.436 | 78 | 38 | 1 | 29 | 106 | 87 | 161 | 9.48e-17 | 75.1 |
| MS.gene067208.t1 | AT4G10920 | 47.436 | 78 | 38 | 1 | 29 | 106 | 87 | 161 | 9.48e-17 | 75.1 |
Find 58 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCGAAATTGATGTGATTT+TGG | 0.193141 | 6.2:-22074837 | MS.gene067208:CDS |
| ATCTATATTTGCCTCTTTCT+TGG | 0.229552 | 6.2:+22074958 | None:intergenic |
| TTTGAATTGTAACTTTCTTT+TGG | 0.278276 | 6.2:+22075913 | None:intergenic |
| GATTTGAAAACGAAAGAAAT+AGG | 0.298954 | 6.2:-22074938 | MS.gene067208:CDS |
| GGATCAGTGGTCCATCTTAA+AGG | 0.299308 | 6.2:-22075068 | MS.gene067208:CDS |
| AGAAGAAGCATATATAAAAC+TGG | 0.300076 | 6.2:-22075029 | MS.gene067208:CDS |
| CAAGAAAGAATATGCTAAAA+TGG | 0.320277 | 6.2:-22074903 | MS.gene067208:CDS |
| GGGACCCTCCCAGTTCTGAC+TGG | 0.366748 | 6.2:+22074999 | None:intergenic |
| AGACAAAATTTGTCTCCATT+AGG | 0.391141 | 6.2:-22075872 | MS.gene067208:CDS |
| GGGAGGGTCCCAGTCAGAAC+TGG | 0.394937 | 6.2:-22075008 | MS.gene067208:CDS |
| TTCGGAGAAGACTGGATTGC+AGG | 0.395516 | 6.2:-22074684 | MS.gene067208:CDS |
| ATACTTTGCAGATGAATAGA+TGG | 0.400434 | 6.2:+22076047 | None:intergenic |
| GGATCGTAGACAGCTGATTT+CGG | 0.401576 | 6.2:-22074702 | MS.gene067208:CDS |
| CATTGGAGGAGAGGTGGAAT+AGG | 0.405658 | 6.2:-22074751 | MS.gene067208:CDS |
| TAACCTTCTTCTCGGCAATC+TGG | 0.406973 | 6.2:+22074726 | None:intergenic |
| AGACTGGATTGCAGGCATTT+CGG | 0.409767 | 6.2:-22074676 | MS.gene067208:CDS |
| TGGCACGCTTTGTTTCTGAC+TGG | 0.413267 | 6.2:+22074978 | None:intergenic |
| TGAACATGGACGACGTGAAA+AGG | 0.420395 | 6.2:-22074808 | MS.gene067208:CDS |
| GGCACGCTTTGTTTCTGACT+GGG | 0.423241 | 6.2:+22074979 | None:intergenic |
| ACGATCCATAACCTTCTTCT+CGG | 0.447059 | 6.2:+22074718 | None:intergenic |
| ATATATGCTTCTTCTATGGC+AGG | 0.452325 | 6.2:+22075037 | None:intergenic |
| TGGTGAGCTTGTAGCAGAAA+TGG | 0.453570 | 6.2:-22076128 | MS.gene067208:CDS |
| ATCTGAGAGGATTGCATTGG+AGG | 0.457412 | 6.2:-22074765 | MS.gene067208:CDS |
| GATTGCCGAGAAGAAGGTTA+TGG | 0.458622 | 6.2:-22074723 | MS.gene067208:CDS |
| TCGTCAGGCACAAGAGGAAC+TGG | 0.467093 | 6.2:-22076148 | MS.gene067208:CDS |
| GAAAAGGGGATTGGAATTGA+TGG | 0.468138 | 6.2:-22074792 | MS.gene067208:CDS |
| AAAACGAAAGAAATAGGTTG+TGG | 0.469430 | 6.2:-22074932 | MS.gene067208:CDS |
| GAACATGGACGACGTGAAAA+GGG | 0.481916 | 6.2:-22074807 | MS.gene067208:CDS |
| CAGCTGATTTCGGAGAAGAC+TGG | 0.483314 | 6.2:-22074692 | MS.gene067208:CDS |
| AGAAATGGATGATGCTCAAA+TGG | 0.487326 | 6.2:-22076113 | MS.gene067208:CDS |
| AGGCATATTTGCCTTTAAGA+TGG | 0.494076 | 6.2:+22075057 | None:intergenic |
| GTTCCAGATTGCCGAGAAGA+AGG | 0.495198 | 6.2:-22074729 | MS.gene067208:CDS |
| TATTAGACAATGTCTTCGTC+AGG | 0.500087 | 6.2:-22076163 | None:intergenic |
| AACAAAGCGTGCCAAGAAAG+AGG | 0.509036 | 6.2:-22074969 | MS.gene067208:CDS |
| GGATTGCATTGGAGGAGAGG+TGG | 0.517797 | 6.2:-22074757 | MS.gene067208:CDS |
| GGACGACGTGAAAAGGGGAT+TGG | 0.526254 | 6.2:-22074801 | MS.gene067208:CDS |
| GGACCCTCCCAGTTCTGACT+GGG | 0.529301 | 6.2:+22075000 | None:intergenic |
| TGATGCTCAAATGGAAGAAG+AGG | 0.544967 | 6.2:-22076104 | MS.gene067208:CDS |
| AAAAGGGGATTGGAATTGAT+GGG | 0.547783 | 6.2:-22074791 | MS.gene067208:CDS |
| GGAGGGTCCCAGTCAGAACT+GGG | 0.548617 | 6.2:-22075007 | MS.gene067208:CDS |
| AGAGGATTGCATTGGAGGAG+AGG | 0.560680 | 6.2:-22074760 | MS.gene067208:CDS |
| GAAATCTGAGAGGATTGCAT+TGG | 0.563890 | 6.2:-22074768 | MS.gene067208:CDS |
| AAAGAATATGCTAAAATGGA+AGG | 0.575746 | 6.2:-22074899 | MS.gene067208:CDS |
| TTTAGGAATAAGTTTGACTG+TGG | 0.581440 | 6.2:-22075089 | MS.gene067208:intron |
| CAATTCAAAAGCGGAATGAG+TGG | 0.584388 | 6.2:-22075899 | MS.gene067208:CDS |
| ATGTCTTCGTCAGGCACAAG+AGG | 0.600940 | 6.2:-22076154 | MS.gene067208:CDS |
| AACATGGACGACGTGAAAAG+GGG | 0.602059 | 6.2:-22074806 | MS.gene067208:CDS |
| TAAGTTTGACTGTGGATCAG+TGG | 0.608779 | 6.2:-22075081 | MS.gene067208:CDS |
| GAATATGCTAAAATGGAAGG+TGG | 0.613411 | 6.2:-22074896 | MS.gene067208:CDS |
| GGTCCCAGTCAGAACTGGGA+GGG | 0.621909 | 6.2:-22075003 | MS.gene067208:CDS |
| GGGTCCCAGTCAGAACTGGG+AGG | 0.629574 | 6.2:-22075004 | MS.gene067208:CDS |
| AGAAAGTTACAATTCAAAAG+CGG | 0.632985 | 6.2:-22075908 | MS.gene067208:CDS |
| GAAGAAGCATATATAAAACT+GGG | 0.640059 | 6.2:-22075028 | MS.gene067208:CDS |
| GTGGAATGTCCTTCTAACCC+AGG | 0.643475 | 6.2:-22075828 | MS.gene067208:intron |
| AAGCATATATAAAACTGGGA+GGG | 0.653918 | 6.2:-22075024 | MS.gene067208:CDS |
| GAAGCATATATAAAACTGGG+AGG | 0.661078 | 6.2:-22075025 | MS.gene067208:CDS |
| AAAGGGGATTGGAATTGATG+GGG | 0.671071 | 6.2:-22074790 | MS.gene067208:CDS |
| AATTGATGGGGAAATCTGAG+AGG | 0.674174 | 6.2:-22074778 | MS.gene067208:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTTTTTTTTATTTATCTA+AGG | + | chr6.2:22074832-22074851 | None:intergenic | 10.0% |
| !! | AAAAAAGTAATTTCAAAAGT+AGG | - | chr6.2:22075592-22075611 | MS.gene067208:intron | 15.0% |
| !!! | AAAAAAAAAAGTACAACTTT+AGG | - | chr6.2:22075302-22075321 | MS.gene067208:intron | 15.0% |
| !! | TAAAATTACAGATAAAGTCA+AGG | + | chr6.2:22075637-22075656 | None:intergenic | 20.0% |
| !! | TTTAAAAACAAACTTCCTAA+TGG | + | chr6.2:22074961-22074980 | None:intergenic | 20.0% |
| !!! | CTTTTTCTACTTGTTTATTT+TGG | - | chr6.2:22074797-22074816 | MS.gene067208:CDS | 20.0% |
| !!! | TAAAAAGTATGTAAACTTTG+AGG | + | chr6.2:22075126-22075145 | None:intergenic | 20.0% |
| !!! | TTTGAATTGTAACTTTCTTT+TGG | + | chr6.2:22074905-22074924 | None:intergenic | 20.0% |
| !!! | TTTTATATATGCTTCTTCTA+TGG | + | chr6.2:22075785-22075804 | None:intergenic | 20.0% |
| ! | AAAATTTAAGTACACATCCT+TGG | - | chr6.2:22075532-22075551 | MS.gene067208:intron | 25.0% |
| ! | AAAGAATATGCTAAAATGGA+AGG | - | chr6.2:22075916-22075935 | MS.gene067208:CDS | 25.0% |
| ! | AGAAAACAATAAAACCCAAA+TGG | + | chr6.2:22075175-22075194 | None:intergenic | 25.0% |
| ! | AGAAAGTTACAATTCAAAAG+CGG | - | chr6.2:22074907-22074926 | MS.gene067208:CDS | 25.0% |
| ! | CAAGAAAGAATATGCTAAAA+TGG | - | chr6.2:22075912-22075931 | MS.gene067208:CDS | 25.0% |
| ! | GAAAACAATAAAACCCAAAT+GGG | + | chr6.2:22075174-22075193 | None:intergenic | 25.0% |
| ! | GATTTGAAAACGAAAGAAAT+AGG | - | chr6.2:22075877-22075896 | MS.gene067208:CDS | 25.0% |
| ! | TATAACACAAAATCAGAGTT+AGG | - | chr6.2:22075503-22075522 | MS.gene067208:intron | 25.0% |
| ! | TTCTTAAGATTGTGTTTGTT+AGG | + | chr6.2:22075467-22075486 | None:intergenic | 25.0% |
| !! | AGAAGAAGCATATATAAAAC+TGG | - | chr6.2:22075786-22075805 | MS.gene067208:intron | 25.0% |
| !! | ATTCGAAATTGATGTGATTT+TGG | - | chr6.2:22075978-22075997 | MS.gene067208:intron | 25.0% |
| !! | GAAGAAGCATATATAAAACT+GGG | - | chr6.2:22075787-22075806 | MS.gene067208:intron | 25.0% |
| !!! | ACTTTCTTTTTCAAGTAACA+TGG | + | chr6.2:22075103-22075122 | None:intergenic | 25.0% |
| !!! | AGGAAGTTTGTTTTTAAACA+CGG | - | chr6.2:22074963-22074982 | MS.gene067208:CDS | 25.0% |
| !!! | CATTTTAGCATATTCTTTCT+TGG | + | chr6.2:22075914-22075933 | None:intergenic | 25.0% |
| !!! | CTTTTTTTATCTTGTCACTA+AGG | + | chr6.2:22075579-22075598 | None:intergenic | 25.0% |
| !!! | TTTCTATTTTTCTGCGTTTT+AGG | - | chr6.2:22075709-22075728 | MS.gene067208:intron | 25.0% |
| AAAACGAAAGAAATAGGTTG+TGG | - | chr6.2:22075883-22075902 | MS.gene067208:CDS | 30.0% | |
| AAGCATATATAAAACTGGGA+GGG | - | chr6.2:22075791-22075810 | MS.gene067208:intron | 30.0% | |
| ACAAAACTAAAATGACATCG+AGG | + | chr6.2:22075044-22075063 | None:intergenic | 30.0% | |
| AGACAAAATTTGTCTCCATT+AGG | - | chr6.2:22074943-22074962 | MS.gene067208:CDS | 30.0% | |
| AGTAACATGGATAAACTTTG+AGG | + | chr6.2:22075090-22075109 | None:intergenic | 30.0% | |
| ATACTTTGCAGATGAATAGA+TGG | + | chr6.2:22074771-22074790 | None:intergenic | 30.0% | |
| ATCTATATTTGCCTCTTTCT+TGG | + | chr6.2:22075860-22075879 | None:intergenic | 30.0% | |
| CAATCTTACCATATGTACTA+TGG | + | chr6.2:22075415-22075434 | None:intergenic | 30.0% | |
| GCAAATAACCATAGTACATA+TGG | - | chr6.2:22075404-22075423 | MS.gene067208:intron | 30.0% | |
| GTGTTATATTTGAGTGTTTC+AGG | + | chr6.2:22075491-22075510 | None:intergenic | 30.0% | |
| TTTAGGAATAAGTTTGACTG+TGG | - | chr6.2:22075726-22075745 | MS.gene067208:intron | 30.0% | |
| ! | GTATGCATTTTTAATCTCCA+AGG | + | chr6.2:22075552-22075571 | None:intergenic | 30.0% |
| ! | TTAAGATTGTGTTTGTTAGG+AGG | + | chr6.2:22075464-22075483 | None:intergenic | 30.0% |
| !!! | TGGGTTTTATTGTTTTCTGT+TGG | - | chr6.2:22075177-22075196 | MS.gene067208:intron | 30.0% |
| AAAAGGGGATTGGAATTGAT+GGG | - | chr6.2:22076024-22076043 | MS.gene067208:intron | 35.0% | |
| AGAAATGGATGATGCTCAAA+TGG | - | chr6.2:22074702-22074721 | MS.gene067208:CDS | 35.0% | |
| AGACAACATGCACCATATAT+AGG | + | chr6.2:22075208-22075227 | None:intergenic | 35.0% | |
| AGATAAAGTCAAGGATGTGT+AGG | + | chr6.2:22075628-22075647 | None:intergenic | 35.0% | |
| ATATATGCTTCTTCTATGGC+AGG | + | chr6.2:22075781-22075800 | None:intergenic | 35.0% | |
| GAAGCATATATAAAACTGGG+AGG | - | chr6.2:22075790-22075809 | MS.gene067208:intron | 35.0% | |
| GAATATGCTAAAATGGAAGG+TGG | - | chr6.2:22075919-22075938 | MS.gene067208:CDS | 35.0% | |
| GTCATCTTTGATTCCCATTT+GGG | - | chr6.2:22075158-22075177 | MS.gene067208:intron | 35.0% | |
| TGTCATCTTTGATTCCCATT+TGG | - | chr6.2:22075157-22075176 | MS.gene067208:intron | 35.0% | |
| ! | AAAGCACAATGCATTTTACC+TGG | + | chr6.2:22075008-22075027 | None:intergenic | 35.0% |
| ! | AAGCACAATGCATTTTACCT+GGG | + | chr6.2:22075007-22075026 | None:intergenic | 35.0% |
| ! | AGGCATATTTGCCTTTAAGA+TGG | + | chr6.2:22075761-22075780 | None:intergenic | 35.0% |
| ! | CGAAATTGATGTGATTTTGG+CGG | - | chr6.2:22075981-22076000 | MS.gene067208:intron | 35.0% |
| !! | AAATTGATGTGATTTTGGCG+GGG | - | chr6.2:22075983-22076002 | MS.gene067208:intron | 35.0% |
| !! | GAAATTGATGTGATTTTGGC+GGG | - | chr6.2:22075982-22076001 | MS.gene067208:intron | 35.0% |
| !!! | GTTTGTTTTTAAACACGGAG+TGG | - | chr6.2:22074968-22074987 | MS.gene067208:CDS | 35.0% |
| AAAGGGGATTGGAATTGATG+GGG | - | chr6.2:22076025-22076044 | MS.gene067208:intron | 40.0% | |
| AATTGATGGGGAAATCTGAG+AGG | - | chr6.2:22076037-22076056 | MS.gene067208:intron | 40.0% | |
| ACGATCCATAACCTTCTTCT+CGG | + | chr6.2:22076100-22076119 | None:intergenic | 40.0% | |
| CAACATGCACCATATATAGG+AGG | + | chr6.2:22075205-22075224 | None:intergenic | 40.0% | |
| CAATTCAAAAGCGGAATGAG+TGG | - | chr6.2:22074916-22074935 | MS.gene067208:CDS | 40.0% | |
| GAAAAGGGGATTGGAATTGA+TGG | - | chr6.2:22076023-22076042 | MS.gene067208:intron | 40.0% | |
| GAAATCTGAGAGGATTGCAT+TGG | - | chr6.2:22076047-22076066 | MS.gene067208:intron | 40.0% | |
| TAAGTTTGACTGTGGATCAG+TGG | - | chr6.2:22075734-22075753 | MS.gene067208:intron | 40.0% | |
| TGATGCTCAAATGGAAGAAG+AGG | - | chr6.2:22074711-22074730 | MS.gene067208:CDS | 40.0% | |
| !! | TGCATTTTACCTGGGTTAGA+AGG | + | chr6.2:22074999-22075018 | None:intergenic | 40.0% |
| AACAAAGCGTGCCAAGAAAG+AGG | - | chr6.2:22075846-22075865 | MS.gene067208:CDS | 45.0% | |
| AACATGGACGACGTGAAAAG+GGG | - | chr6.2:22076009-22076028 | MS.gene067208:intron | 45.0% | |
| AGACTGGATTGCAGGCATTT+CGG | - | chr6.2:22076139-22076158 | MS.gene067208:CDS | 45.0% | |
| ATCTGAGAGGATTGCATTGG+AGG | - | chr6.2:22076050-22076069 | MS.gene067208:intron | 45.0% | |
| GAACATGGACGACGTGAAAA+GGG | - | chr6.2:22076008-22076027 | MS.gene067208:intron | 45.0% | |
| GATTGCCGAGAAGAAGGTTA+TGG | - | chr6.2:22076092-22076111 | MS.gene067208:CDS | 45.0% | |
| GGATCAGTGGTCCATCTTAA+AGG | - | chr6.2:22075747-22075766 | MS.gene067208:intron | 45.0% | |
| TAACCTTCTTCTCGGCAATC+TGG | + | chr6.2:22076092-22076111 | None:intergenic | 45.0% | |
| TGAACATGGACGACGTGAAA+AGG | - | chr6.2:22076007-22076026 | MS.gene067208:intron | 45.0% | |
| TGGTGAGCTTGTAGCAGAAA+TGG | - | chr6.2:22074687-22074706 | MS.gene067208:CDS | 45.0% | |
| ! | CTGTTGGTGCCTCCTATATA+TGG | - | chr6.2:22075193-22075212 | MS.gene067208:intron | 45.0% |
| ! | GGATCGTAGACAGCTGATTT+CGG | - | chr6.2:22076113-22076132 | MS.gene067208:CDS | 45.0% |
| AGAGGATTGCATTGGAGGAG+AGG | - | chr6.2:22076055-22076074 | MS.gene067208:CDS | 50.0% | |
| ATGTCTTCGTCAGGCACAAG+AGG | - | chr6.2:22074661-22074680 | MS.gene067208:CDS | 50.0% | |
| CATTGGAGGAGAGGTGGAAT+AGG | - | chr6.2:22076064-22076083 | MS.gene067208:CDS | 50.0% | |
| GTGGAATGTCCTTCTAACCC+AGG | - | chr6.2:22074987-22075006 | MS.gene067208:CDS | 50.0% | |
| GTTCCAGATTGCCGAGAAGA+AGG | - | chr6.2:22076086-22076105 | MS.gene067208:CDS | 50.0% | |
| TTCGGAGAAGACTGGATTGC+AGG | - | chr6.2:22076131-22076150 | MS.gene067208:CDS | 50.0% | |
| ! | CAGCTGATTTCGGAGAAGAC+TGG | - | chr6.2:22076123-22076142 | MS.gene067208:CDS | 50.0% |
| !! | GGCACGCTTTGTTTCTGACT+GGG | + | chr6.2:22075839-22075858 | None:intergenic | 50.0% |
| !! | TGGCACGCTTTGTTTCTGAC+TGG | + | chr6.2:22075840-22075859 | None:intergenic | 50.0% |
| GGACGACGTGAAAAGGGGAT+TGG | - | chr6.2:22076014-22076033 | MS.gene067208:intron | 55.0% | |
| GGATTGCATTGGAGGAGAGG+TGG | - | chr6.2:22076058-22076077 | MS.gene067208:CDS | 55.0% | |
| TCGTCAGGCACAAGAGGAAC+TGG | - | chr6.2:22074667-22074686 | MS.gene067208:CDS | 55.0% | |
| !! | GATTTTGGCGGGGCTGAACA+TGG | - | chr6.2:22075993-22076012 | MS.gene067208:intron | 55.0% |
| GGAGGGTCCCAGTCAGAACT+GGG | - | chr6.2:22075808-22075827 | MS.gene067208:intron | 60.0% | |
| GGTCCCAGTCAGAACTGGGA+GGG | - | chr6.2:22075812-22075831 | MS.gene067208:intron | 60.0% | |
| ! | GGACCCTCCCAGTTCTGACT+GGG | + | chr6.2:22075818-22075837 | None:intergenic | 60.0% |
| GGGAGGGTCCCAGTCAGAAC+TGG | - | chr6.2:22075807-22075826 | MS.gene067208:intron | 65.0% | |
| GGGTCCCAGTCAGAACTGGG+AGG | - | chr6.2:22075811-22075830 | MS.gene067208:intron | 65.0% | |
| ! | GGGACCCTCCCAGTTCTGAC+TGG | + | chr6.2:22075819-22075838 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 22074661 | 22076176 | 22074661 | ID=MS.gene067208 |
| chr6.2 | mRNA | 22074661 | 22076176 | 22074661 | ID=MS.gene067208.t1;Parent=MS.gene067208 |
| chr6.2 | exon | 22076051 | 22076176 | 22076051 | ID=MS.gene067208.t1.exon1;Parent=MS.gene067208.t1 |
| chr6.2 | CDS | 22076051 | 22076176 | 22076051 | ID=cds.MS.gene067208.t1;Parent=MS.gene067208.t1 |
| chr6.2 | exon | 22075829 | 22075943 | 22075829 | ID=MS.gene067208.t1.exon2;Parent=MS.gene067208.t1 |
| chr6.2 | CDS | 22075829 | 22075943 | 22075829 | ID=cds.MS.gene067208.t1;Parent=MS.gene067208.t1 |
| chr6.2 | exon | 22074661 | 22075106 | 22074661 | ID=MS.gene067208.t1.exon3;Parent=MS.gene067208.t1 |
| chr6.2 | CDS | 22074661 | 22075106 | 22074661 | ID=cds.MS.gene067208.t1;Parent=MS.gene067208.t1 |
| Gene Sequence |
| Protein sequence |
