AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071342


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071342.t1 MTR_2g060720 98.540 137 2 0 1 137 297 433 7.00e-93 275
MS.gene071342.t1 MTR_2g060720 91.176 136 12 0 1 136 297 432 2.04e-84 254
MS.gene071342.t1 MTR_4g022860 73.571 140 33 3 1 137 293 431 1.80e-50 166
MS.gene071342.t1 MTR_1g053905 60.465 129 46 3 1 125 198 325 3.74e-45 150
MS.gene071342.t1 MTR_4g100880 45.255 137 47 3 1 137 175 283 1.08e-30 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071342.t1 AT2G44440 50.781 128 61 1 1 128 303 428 4.11e-38 134
MS.gene071342.t1 AT5G13020 52.381 126 53 3 1 123 273 394 1.86e-33 120
MS.gene071342.t1 AT5G06780 49.219 128 56 3 1 125 179 300 7.08e-31 112
MS.gene071342.t1 AT5G06780 49.219 128 56 3 1 125 186 307 7.55e-31 112
MS.gene071342.t1 AT3G12140 53.077 130 54 4 1 125 187 314 1.63e-24 96.3
MS.gene071342.t1 AT3G12140 53.077 130 54 4 1 125 187 314 1.63e-24 96.3
MS.gene071342.t1 AT3G12140 53.846 130 47 6 1 122 187 311 3.16e-23 93.6

Find 0 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAATTAAAATGCTA+TGG + 16771:2007-2026 MS.gene071342:intron 10.0%
!!! ATGGAAAATATTTAAATTTT+TGG + 16771:1652-1671 MS.gene071342:intron 10.0%
!!! TGGAAAATATTTAAATTTTT+GGG + 16771:1653-1672 MS.gene071342:intron 10.0%
!!! GCATTTTAATTTTAATTTAG+AGG - 16771:2005-2024 MS.gene071342:intergenic 15.0%
!! AATACTGCAAAAATGTAAAT+AGG - 16771:1954-1973 MS.gene071342:intergenic 20.0%
!! ATAGACAAAAGTGAAAAATA+TGG - 16771:2379-2398 MS.gene071342:intergenic 20.0%
!! TTTAAAAAAGTAGTGAATAC+TGG - 16771:2426-2445 MS.gene071342:intergenic 20.0%
!!! TAAATAGAAGTACAATAGTT+TGG + 16771:2199-2218 MS.gene071342:intron 20.0%
! AATTAAAATGCTATGGAGTA+TGG + 16771:2014-2033 MS.gene071342:intron 25.0%
! ATATTAAATCGTAGGATGAT+TGG - 16771:2339-2358 MS.gene071342:intergenic 25.0%
! ATCAATTATATTCAGTGAAG+TGG + 16771:2457-2476 MS.gene071342:intron 25.0%
! ATCTTATTCTTTATGCAGAT+GGG + 16771:2541-2560 MS.gene071342:intron 25.0%
! CAAGAAAATATTGAAGGTAA+AGG + 16771:1740-1759 MS.gene071342:intron 25.0%
! CAATTTAATGAATTAACTGC+AGG + 16771:1843-1862 MS.gene071342:intron 25.0%
! TATCTTATTCTTTATGCAGA+TGG + 16771:2540-2559 MS.gene071342:intron 25.0%
! TGCAAGAACATATAAATTAG+CGG - 16771:1806-1825 MS.gene071342:intergenic 25.0%
! TTGATTTAACATGTGAGAAA+TGG - 16771:2575-2594 MS.gene071342:intergenic 25.0%
! TTTCTCACATGTTAAATCAA+TGG + 16771:2575-2594 MS.gene071342:CDS 25.0%
! TTTGTCTATTTAAACAACCT+TGG + 16771:2390-2409 MS.gene071342:intron 25.0%
!! CTTTACCTTCAATATTTTCT+TGG - 16771:1742-1761 MS.gene071342:intergenic 25.0%
!! GAGATTGTTTAATCTTTTCA+TGG + 16771:1633-1652 MS.gene071342:intron 25.0%
!! TACAATAGTTTGGAATAACA+AGG + 16771:2209-2228 MS.gene071342:intron 25.0%
!!! TGATGGCAATAAATCTTTTT+TGG - 16771:1507-1526 MS.gene071342:intergenic 25.0%
ACGATTTAATATACCAAGCA+GGG + 16771:2347-2366 MS.gene071342:intron 30.0%
GAAAGCCAAGAAAATATTGA+AGG + 16771:1734-1753 MS.gene071342:CDS 30.0%
GTTAAAGAGGTAACTGAAAA+CGG + 16771:1587-1606 MS.gene071342:intron 30.0%
TACGATTTAATATACCAAGC+AGG + 16771:2346-2365 MS.gene071342:intron 30.0%
TAGTGAATACTGGATACTTT+AGG - 16771:2416-2435 MS.gene071342:intergenic 30.0%
TCTTATTCTTTATGCAGATG+GGG + 16771:2542-2561 MS.gene071342:intron 30.0%
TGCTTGGTATATTAAATCGT+AGG - 16771:2347-2366 MS.gene071342:intergenic 30.0%
TTCAAAGTGAACTAGCTAAA+TGG - 16771:2131-2150 MS.gene071342:intergenic 30.0%
TTCTCAATTTCATGTACATC+AGG - 16771:1717-1736 MS.gene071342:intergenic 30.0%
TTTAGCTAGTTCACTTTGAA+AGG + 16771:2131-2150 MS.gene071342:intron 30.0%
TTTATACACAGAATCAAGTG+TGG - 16771:2505-2524 MS.gene071342:intergenic 30.0%
!! GATTTTCACTGATTATTGCT+TGG + 16771:2236-2255 MS.gene071342:intron 30.0%
!!! AAATTTTTGGGTAACTTGTC+AGG + 16771:1665-1684 MS.gene071342:intron 30.0%
CAAATGAGTTGCAAGTGATA+CGG + 16771:2083-2102 MS.gene071342:intron 35.0%
CAGTAAAATAAATGGCAGAC+TGG - 16771:1779-1798 MS.gene071342:intergenic 35.0%
CGATTTAATATACCAAGCAG+GGG + 16771:2348-2367 MS.gene071342:intron 35.0%
CTTATTCTTTATGCAGATGG+GGG + 16771:2543-2562 MS.gene071342:intron 35.0%
GATTTATTGCCATCACAGAA+TGG + 16771:1512-1531 MS.gene071342:CDS 35.0%
AATCGTAGGATGATTGGAAG+TGG - 16771:2333-2352 None:intergenic 40.0%
ACAGAATGGAATCGGGAAAA+AGG + 16771:1526-1545 MS.gene071342:CDS 40.0%
ATACTGGATACTTTAGGCCA+AGG - 16771:2410-2429 MS.gene071342:intergenic 40.0%
ATCGTAGGATGATTGGAAGT+GGG - 16771:2332-2351 MS.gene071342:intergenic 40.0%
CACAGACACACTAGTTAAAG+AGG + 16771:1574-1593 MS.gene071342:CDS 40.0%
CCTACCCATTGAATATCTTC+CGG - 16771:1368-1387 MS.gene071342:intergenic 40.0%
CGGAAGATATTCAATGGGTA+GGG + 16771:1366-1385 MS.gene071342:CDS 40.0%
CTCTCCGGAAGATATTCAAT+GGG + 16771:1361-1380 MS.gene071342:CDS 40.0%
CTCTTATCGATGCAATTGCA+AGG + 16771:1878-1897 MS.gene071342:CDS 40.0%
GCGGCTAGCAGTAAAATAAA+TGG - 16771:1787-1806 MS.gene071342:intergenic 40.0%
GGAAGATATTCAATGGGTAG+GGG + 16771:1367-1386 MS.gene071342:CDS 40.0%
TCTCTCCGGAAGATATTCAA+TGG + 16771:1360-1379 MS.gene071342:CDS 40.0%
TTGCCATCACAGAATGGAAT+CGG + 16771:1518-1537 MS.gene071342:CDS 40.0%
! CTGGAATCAACCATCGTTTT+GGG + 16771:1396-1415 MS.gene071342:CDS 40.0%
! GATCTTTCTGATGGCGATAA+TGG + 16771:1907-1926 MS.gene071342:CDS 40.0%
! TGGAATCAACCATCGTTTTG+GGG + 16771:1397-1416 MS.gene071342:CDS 40.0%
!!! TTTTTGGGTAACTTGTCAGG+TGG + 16771:1668-1687 MS.gene071342:intron 40.0%
AATATGGAATCACCCCTGCT+TGG - 16771:2363-2382 None:intergenic 45.0%
AGATATTCAATGGGTAGGGG+AGG + 16771:1370-1389 MS.gene071342:CDS 45.0%
AGGCTTGCAGATCTTTCTGA+TGG + 16771:1898-1917 MS.gene071342:CDS 45.0%
ATTCTTTATGCAGATGGGGG+CGG + 16771:2546-2565 MS.gene071342:intron 45.0%
CCGGAAGATATTCAATGGGT+AGG + 16771:1365-1384 MS.gene071342:CDS 45.0%
GAGATCCATTGTTCCATCAC+TGG - 16771:2483-2502 MS.gene071342:intergenic 45.0%
GATTATGGCCACTGTTTCCA+CGG - 16771:2296-2315 MS.gene071342:intergenic 45.0%
TCGTAGGATGATTGGAAGTG+GGG - 16771:2331-2350 MS.gene071342:intergenic 45.0%
TGCCATCACAGAATGGAATC+GGG + 16771:1519-1538 MS.gene071342:CDS 45.0%
TTCATCTTACTTCCATGCCG+TGG + 16771:2276-2295 MS.gene071342:intron 45.0%
TTCCCGATTCCATTCTGTGA+TGG - 16771:1524-1543 MS.gene071342:intergenic 45.0%
! CCAAAACGATGGTTGATTCC+AGG - 16771:1398-1417 MS.gene071342:intergenic 45.0%
! CCTGGAATCAACCATCGTTT+TGG + 16771:1395-1414 MS.gene071342:CDS 45.0%
! GGAATCAACCATCGTTTTGG+GGG + 16771:1398-1417 MS.gene071342:CDS 45.0%
! GGGTGTAGTGGAACAGATTA+TGG - 16771:2311-2330 MS.gene071342:intergenic 45.0%
! GGTAACTTGTCAGGTGGAAA+GGG + 16771:1674-1693 MS.gene071342:intron 45.0%
TGATTGGAAGTGGGGTGTAG+TGG - 16771:2323-2342 MS.gene071342:intergenic 50.0%
TGGAATCGGGAAAAAGGCTC+TGG + 16771:1532-1551 MS.gene071342:CDS 50.0%
TTCAGTGAAGTGGCCAGTGA+TGG + 16771:2467-2486 MS.gene071342:intron 50.0%
! AAGAGGTAGGGGAGGTACAA+AGG + 16771:1475-1494 MS.gene071342:CDS 50.0%
! AGAGGTAGGGGAGGTACAAA+GGG + 16771:1476-1495 MS.gene071342:CDS 50.0%
! AGTGGCCAGTGATGGAACAA+TGG + 16771:2475-2494 MS.gene071342:intron 50.0%
! CGTGGGTTGAATAGGTCTGT+AGG + 16771:1428-1447 MS.gene071342:CDS 50.0%
! GATCTGGTCGTGGGTTGAAT+AGG + 16771:1420-1439 MS.gene071342:CDS 50.0%
! GGGTAACTTGTCAGGTGGAA+AGG + 16771:1673-1692 MS.gene071342:intron 50.0%
!!! AACCATCGTTTTGGGGGATC+TGG + 16771:1404-1423 MS.gene071342:CDS 50.0%
AGTGTTCCAGGAGCTGGAAG+AGG + 16771:1458-1477 MS.gene071342:CDS 55.0%
CTTCCATGCCGTGGAAACAG+TGG + 16771:2285-2304 MS.gene071342:intron 55.0%
GACCAGATCCCCCAAAACGA+TGG - 16771:1409-1428 MS.gene071342:intergenic 55.0%
GTAGGTCGCGATAGTGTTCC+AGG + 16771:1446-1465 MS.gene071342:CDS 55.0%
TCCAGGAGCTGGAAGAGGTA+GGG + 16771:1463-1482 MS.gene071342:CDS 55.0%
TTCCAGGAGCTGGAAGAGGT+AGG + 16771:1462-1481 MS.gene071342:CDS 55.0%
! GAGGTAGGGGAGGTACAAAG+GGG + 16771:1477-1496 MS.gene071342:CDS 55.0%
!!! GTTTTGGGGGATCTGGTCGT+GGG + 16771:1411-1430 MS.gene071342:CDS 55.0%
CAATGGGTAGGGGAGGAACC+TGG + 16771:1377-1396 MS.gene071342:CDS 60.0%
CCAGGAGCTGGAAGAGGTAG+GGG + 16771:1464-1483 MS.gene071342:CDS 60.0%
CGCGATAGTGTTCCAGGAGC+TGG + 16771:1452-1471 MS.gene071342:CDS 60.0%
TGGCCACTGTTTCCACGGCA+TGG - 16771:2291-2310 MS.gene071342:intergenic 60.0%
!!! CGTTTTGGGGGATCTGGTCG+TGG + 16771:1410-1429 MS.gene071342:CDS 60.0%
CCCCTACCTCTTCCAGCTCC+TGG - 16771:1467-1486 MS.gene071342:intergenic 65.0%
GGAGCTGGAAGAGGTAGGGG+AGG + 16771:1467-1486 MS.gene071342:CDS 65.0%


Chromosome Type Strat End Strand Name
16771 gene 1359 2602 1359 ID=MS.gene071342
16771 mRNA 1359 2602 1359 ID=MS.gene071342.t1;Parent=MS.gene071342
16771 exon 1359 1595 1359 ID=MS.gene071342.t1.exon1;Parent=MS.gene071342.t1
16771 CDS 1359 1595 1359 ID=cds.MS.gene071342.t1;Parent=MS.gene071342.t1
16771 exon 1687 1755 1687 ID=MS.gene071342.t1.exon2;Parent=MS.gene071342.t1
16771 CDS 1687 1755 1687 ID=cds.MS.gene071342.t1;Parent=MS.gene071342.t1
16771 exon 1865 1928 1865 ID=MS.gene071342.t1.exon3;Parent=MS.gene071342.t1
16771 CDS 1865 1928 1865 ID=cds.MS.gene071342.t1;Parent=MS.gene071342.t1
16771 exon 2559 2602 2559 ID=MS.gene071342.t1.exon4;Parent=MS.gene071342.t1
16771 CDS 2559 2602 2559 ID=cds.MS.gene071342.t1;Parent=MS.gene071342.t1
Gene Sequence

>MS.gene071342

ATCTCTCCGGAAGATATTCAATGGGTAGGGGAGGAACCTGGAATCAACCATCGTTTTGGGGGATCTGGTCGTGGGTTGAATAGGTCTGTAGGTCGCGATAGTGTTCCAGGAGCTGGAAGAGGTAGGGGAGGTACAAAGGGGCAATCCAAAAAAGATTTATTGCCATCACAGAATGGAATCGGGAAAAAGGCTCTGGATGATATACAAATACTTCACACAGACACACTAGTTAAAGAGGTAACTGAAAACGGTTCAATCAGAACACTATTCAATTGAGATTGTTTAATCTTTTCATGGAAAATATTTAAATTTTTGGGTAACTTGTCAGGTGGAAAGGGTGTTCAGTGCAAATCATCCTGATGTACATGAAATTGAGAAAGCCAAGAAAATATTGAAGGTAAAGGACAATTTTATTCTCCAGTCTGCCATTTATTTTACTGCTAGCCGCTAATTTATATGTTCTTGCAAATGCTGTATGTATAATCAATTTAATGAATTAACTGCAGGATCATGAGCAAGCTCTTATCGATGCAATTGCAAGGCTTGCAGATCTTTCTGATGGCGATAATGGTACTTATTCGCATTTCGCACACCTATTTACATTTTTGCAGTATTATAAGACATTTGTTCTTTCACAAGTAGTCCTCTAAATTAAAATTAAAATGCTATGGAGTATGGACTTCGTTTAGATGCTTTTTTTACTATTTAGTTTCAAATCATAATTCAAATGAGTTGCAAGTGATACGGATGATAATACAGATTAGTTTGTCCATTTAGCTAGTTCACTTTGAAAGGCTAGCATTTTGATTTTAAAAATTACATTGACTGCAAATTTGAGTGTAAATAGAAGTACAATAGTTTGGAATAACAAGGATTTGATTTTCACTGATTATTGCTTGGTATAATAGAAAATCACTTTCATCTTACTTCCATGCCGTGGAAACAGTGGCCATAATCTGTTCCACTACACCCCACTTCCAATCATCCTACGATTTAATATACCAAGCAGGGGTGATTCCATATTTTTCACTTTTGTCTATTTAAACAACCTTGGCCTAAAGTATCCAGTATTCACTACTTTTTTAAAAAAGTGTTGTCATCAATTATATTCAGTGAAGTGGCCAGTGATGGAACAATGGATCTCCACACTTGATTCTGTGTATAAACTTTCTTGATGATCTTATCTTATTCTTTATGCAGATGGGGGCGGTCGCCATTTCTCACATGTTAAATCAATGGATTGA

Protein sequence

>MS.gene071342.t1

ISPEDIQWVGEEPGINHRFGGSGRGLNRSVGRDSVPGAGRGRGGTKGQSKKDLLPSQNGIGKKALDDIQILHTDTLVKEVERVFSANHPDVHEIEKAKKILKDHEQALIDAIARLADLSDGDNDGGGRHFSHVKSMD