Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07815.t1 | XP_003617005.1 | 77.8 | 45 | 10 | 0 | 2 | 46 | 1308 | 1352 | 1.10E-09 | 73.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07815.t1 | G7LI81 | 79.6 | 181 | 37 | 0 | 1 | 181 | 1054 | 1234 | 1.0e-09 | 73.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene07815 | 0.804063 | 2.59E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene07815 | 0.804832 | 1.79E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene07815 | 0.831161 | 1.95E-55 | -1.69E-46 |
| MS.gene049283 | MS.gene07815 | 0.800949 | 1.13E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene07815 | 0.812904 | 3.35E-51 | -1.69E-46 |
| MS.gene049567 | MS.gene07815 | 0.836272 | 1.03E-56 | -1.69E-46 |
| MS.gene049652 | MS.gene07815 | 0.804204 | 2.42E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene07815 | 0.81037 | 1.19E-50 | -1.69E-46 |
| MS.gene050026 | MS.gene07815 | 0.817758 | 2.79E-52 | -1.69E-46 |
| MS.gene050031 | MS.gene07815 | 0.804506 | 2.09E-49 | -1.69E-46 |
| MS.gene050043 | MS.gene07815 | 0.803368 | 3.60E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene07815 | 0.806232 | 9.09E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene07815 | 0.804451 | 2.15E-49 | -1.69E-46 |
| MS.gene051832 | MS.gene07815 | 0.812775 | 3.58E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene07815 | 0.8022 | 6.28E-49 | -1.69E-46 |
| MS.gene052493 | MS.gene07815 | 0.807101 | 5.96E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene07815 | 0.811751 | 5.98E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene07815 | 0.803058 | 4.18E-49 | -1.69E-46 |
| MS.gene052534 | MS.gene07815 | 0.801984 | 6.96E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene07815 | 0.801003 | 1.11E-48 | -1.69E-46 |
| MS.gene052685 | MS.gene07815 | 0.935595 | 6.62E-97 | -1.69E-46 |
| MS.gene052686 | MS.gene07815 | 0.820216 | 7.71E-53 | -1.69E-46 |
| MS.gene053094 | MS.gene07815 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene07815 | 0.836211 | 1.07E-56 | -1.69E-46 |
| MS.gene05365 | MS.gene07815 | 0.816175 | 6.33E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene07815 | 0.86893 | 4.53E-66 | -1.69E-46 |
| MS.gene054222 | MS.gene07815 | 0.845594 | 3.69E-59 | -1.69E-46 |
| MS.gene05423 | MS.gene07815 | 0.802513 | 5.41E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene07815 | 0.800781 | 1.23E-48 | -1.69E-46 |
| MS.gene054576 | MS.gene07815 | 0.813771 | 2.16E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene07815 | 0.810891 | 9.20E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene07815 | 0.823135 | 1.63E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene07815 | 0.821539 | 3.83E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene07815 | 0.814942 | 1.19E-51 | -1.69E-46 |
| MS.gene055589 | MS.gene07815 | 0.80478 | 1.83E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene07815 | 0.815526 | 8.84E-52 | -1.69E-46 |
| MS.gene055801 | MS.gene07815 | 0.844958 | 5.48E-59 | -1.69E-46 |
| MS.gene055840 | MS.gene07815 | 0.800219 | 1.60E-48 | -1.69E-46 |
| MS.gene05636 | MS.gene07815 | 0.805405 | 1.36E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene07815 | 0.804092 | 2.55E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene07815 | 0.806689 | 7.28E-50 | -1.69E-46 |
| MS.gene056779 | MS.gene07815 | 0.817497 | 3.20E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene07815 | 0.815354 | 9.65E-52 | -1.69E-46 |
| MS.gene05690 | MS.gene07815 | 0.813811 | 2.12E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene07815 | 0.829477 | 5.04E-55 | -1.69E-46 |
| MS.gene057155 | MS.gene07815 | 0.803974 | 2.70E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene07815 | 0.814323 | 1.63E-51 | -1.69E-46 |
| MS.gene058008 | MS.gene07815 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene058037 | MS.gene07815 | 0.833096 | 6.49E-56 | -1.69E-46 |
| MS.gene058159 | MS.gene07815 | 0.837485 | 5.06E-57 | -1.69E-46 |
| MS.gene058279 | MS.gene07815 | 0.810217 | 1.29E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene07815 | 0.804798 | 1.82E-49 | -1.69E-46 |
| MS.gene05878 | MS.gene07815 | 0.813831 | 2.10E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene07815 | 0.801139 | 1.04E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene07815 | 0.850606 | 1.52E-60 | -1.69E-46 |
| MS.gene059774 | MS.gene07815 | 0.814878 | 1.23E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene07815 | -0.820498 | 6.65E-53 | -1.69E-46 |
| MS.gene060012 | MS.gene07815 | 0.837866 | 4.04E-57 | -1.69E-46 |
| MS.gene060258 | MS.gene07815 | 0.806457 | 8.15E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene07815 | 0.834175 | 3.49E-56 | -1.69E-46 |
| MS.gene060521 | MS.gene07815 | 0.802149 | 6.43E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene07815 | 0.80913 | 2.20E-50 | -1.69E-46 |
| MS.gene060840 | MS.gene07815 | 0.801181 | 1.02E-48 | -1.69E-46 |
| MS.gene060879 | MS.gene07815 | 0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene07815 | 0.808147 | 3.57E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene07815 | 0.805393 | 1.36E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene07815 | 0.821668 | 3.57E-53 | -1.69E-46 |
| MS.gene061047 | MS.gene07815 | 0.815068 | 1.12E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene07815 | 0.80645 | 8.18E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07815.t1 | MTR_8g018270 | 87.293 | 181 | 23 | 0 | 1 | 181 | 1054 | 1234 | 6.57e-89 | 283 |
| MS.gene07815.t1 | MTR_8g018280 | 75.000 | 180 | 45 | 0 | 2 | 181 | 1294 | 1473 | 1.42e-75 | 246 |
| MS.gene07815.t1 | MTR_5g086690 | 73.256 | 172 | 46 | 0 | 2 | 173 | 1308 | 1479 | 1.14e-70 | 232 |
| MS.gene07815.t1 | MTR_8g018160 | 72.093 | 43 | 12 | 0 | 25 | 67 | 1 | 43 | 1.16e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCCATCAGCTCTTTGCTTT+CGG | 0.279935 | 8.3:-72351210 | None:intergenic |
| ATGGCAATTACATATGTCTT+TGG | 0.310191 | 8.3:+72350794 | None:intergenic |
| CAATAGAGGATGTCATATTA+TGG | 0.331248 | 8.3:-72350872 | None:intergenic |
| TCAACTTCTGCTGCTTTGAC+AGG | 0.334818 | 8.3:-72351268 | None:intergenic |
| ATAGAATTTGCAGACAAGTA+TGG | 0.363847 | 8.3:+72351314 | MS.gene07815:CDS |
| TGGACAAGCTTCACAACATT+AGG | 0.371469 | 8.3:-72350852 | None:intergenic |
| AGCAGAAGTTGAAAGACAAA+TGG | 0.425002 | 8.3:+72351279 | MS.gene07815:CDS |
| GCAAAAGAATATTCTTGAGT+TGG | 0.430621 | 8.3:+72351106 | MS.gene07815:CDS |
| GAAAGAAAAGAGTCAGTTTG+AGG | 0.447261 | 8.3:+72351019 | MS.gene07815:CDS |
| TGAAAGTGAAAAGAAGAAAG+TGG | 0.451582 | 8.3:+72351163 | MS.gene07815:CDS |
| TTCCATGCTCTCCTTCAACT+TGG | 0.474707 | 8.3:-72351135 | None:intergenic |
| GCAGAAGTTGAAAGACAAAT+GGG | 0.480276 | 8.3:+72351280 | MS.gene07815:CDS |
| ATTGAAAAGGTTCTCAATAG+AGG | 0.496397 | 8.3:-72350886 | None:intergenic |
| CAACTTCTGCTGCTTTGACA+GGG | 0.502838 | 8.3:-72351267 | None:intergenic |
| CCATGACGAAGTCATTGAAA+AGG | 0.508796 | 8.3:-72350899 | None:intergenic |
| AGCTAAGCGTGTCGAGGAAG+AGG | 0.527737 | 8.3:+72351046 | MS.gene07815:CDS |
| AACCAAGTTGAAGGAGAGCA+TGG | 0.531090 | 8.3:+72351133 | MS.gene07815:CDS |
| AACCGAAAGCAAAGAGCTGA+TGG | 0.557939 | 8.3:+72351208 | MS.gene07815:CDS |
| AAAGAAGAAAGTGGAAGATG+AGG | 0.561565 | 8.3:+72351172 | MS.gene07815:CDS |
| CGAGGAAGAGGTTGAAAAGA+CGG | 0.567502 | 8.3:+72351058 | MS.gene07815:CDS |
| GACAGATGCTAAGAAACAGA+AGG | 0.616598 | 8.3:+72350986 | MS.gene07815:CDS |
| AAGTTGAAAGACAAATGGGA+AGG | 0.630448 | 8.3:+72351284 | MS.gene07815:CDS |
| GGATGAAGCTAAGCGTGTCG+AGG | 0.666115 | 8.3:+72351040 | MS.gene07815:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAGAATTTGCAGACAAGTA+TGG | + | chr8.3:72351314-72351333 | MS.gene07815:CDS | 30.0% | |
| ATTGAAAAGGTTCTCAATAG+AGG | - | chr8.3:72350889-72350908 | None:intergenic | 30.0% | |
| CAATAGAGGATGTCATATTA+TGG | - | chr8.3:72350875-72350894 | None:intergenic | 30.0% | |
| GCAAAAGAATATTCTTGAGT+TGG | + | chr8.3:72351106-72351125 | MS.gene07815:CDS | 30.0% | |
| TGAAAGTGAAAAGAAGAAAG+TGG | + | chr8.3:72351163-72351182 | MS.gene07815:CDS | 30.0% | |
| AAAGAAGAAAGTGGAAGATG+AGG | + | chr8.3:72351172-72351191 | MS.gene07815:CDS | 35.0% | |
| AAGTTGAAAGACAAATGGGA+AGG | + | chr8.3:72351284-72351303 | MS.gene07815:CDS | 35.0% | |
| AGCAGAAGTTGAAAGACAAA+TGG | + | chr8.3:72351279-72351298 | MS.gene07815:CDS | 35.0% | |
| GAAAGAAAAGAGTCAGTTTG+AGG | + | chr8.3:72351019-72351038 | MS.gene07815:CDS | 35.0% | |
| GCAGAAGTTGAAAGACAAAT+GGG | + | chr8.3:72351280-72351299 | MS.gene07815:CDS | 35.0% | |
| ! | GTTGGAAAAAACCAAGTTGA+AGG | + | chr8.3:72351124-72351143 | MS.gene07815:CDS | 35.0% |
| CATGGCTAAAAATGTGGTTG+AGG | + | chr8.3:72350917-72350936 | MS.gene07815:CDS | 40.0% | |
| CCATGACGAAGTCATTGAAA+AGG | - | chr8.3:72350902-72350921 | None:intergenic | 40.0% | |
| CTTCGTCATGGCTAAAAATG+TGG | + | chr8.3:72350911-72350930 | MS.gene07815:CDS | 40.0% | |
| GACAGATGCTAAGAAACAGA+AGG | + | chr8.3:72350986-72351005 | MS.gene07815:CDS | 40.0% | |
| TGGACAAGCTTCACAACATT+AGG | - | chr8.3:72350855-72350874 | None:intergenic | 40.0% | |
| ! | CCTTTTCAATGACTTCGTCA+TGG | + | chr8.3:72350899-72350918 | MS.gene07815:CDS | 40.0% |
| AACCGAAAGCAAAGAGCTGA+TGG | + | chr8.3:72351208-72351227 | MS.gene07815:CDS | 45.0% | |
| CGAGGAAGAGGTTGAAAAGA+CGG | + | chr8.3:72351058-72351077 | MS.gene07815:CDS | 45.0% | |
| TTCCATGCTCTCCTTCAACT+TGG | - | chr8.3:72351138-72351157 | None:intergenic | 45.0% | |
| ! | AACCAAGTTGAAGGAGAGCA+TGG | + | chr8.3:72351133-72351152 | MS.gene07815:CDS | 45.0% |
| !! | CAACTTCTGCTGCTTTGACA+GGG | - | chr8.3:72351270-72351289 | None:intergenic | 45.0% |
| !! | TCAACTTCTGCTGCTTTGAC+AGG | - | chr8.3:72351271-72351290 | None:intergenic | 45.0% |
| CGCCATCAGCTCTTTGCTTT+CGG | - | chr8.3:72351213-72351232 | None:intergenic | 50.0% | |
| AGCTAAGCGTGTCGAGGAAG+AGG | + | chr8.3:72351046-72351065 | MS.gene07815:CDS | 55.0% | |
| GGATGAAGCTAAGCGTGTCG+AGG | + | chr8.3:72351040-72351059 | MS.gene07815:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 72350807 | 72351352 | 72350807 | ID=MS.gene07815 |
| chr8.3 | mRNA | 72350807 | 72351352 | 72350807 | ID=MS.gene07815.t1;Parent=MS.gene07815 |
| chr8.3 | exon | 72350807 | 72351352 | 72350807 | ID=MS.gene07815.t1.exon1;Parent=MS.gene07815.t1 |
| chr8.3 | CDS | 72350807 | 72351352 | 72350807 | ID=cds.MS.gene07815.t1;Parent=MS.gene07815.t1 |
| Gene Sequence |
| Protein sequence |
