Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75963.t1 | XP_003593377.1 | 44 | 91 | 42 | 2 | 97 | 178 | 319 | 409 | 2.30E-07 | 65.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75963.t1 | O65155 | 48.4 | 64 | 30 | 1 | 1 | 64 | 101 | 161 | 1.5e-07 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75963.t1 | G7IK18 | 44.0 | 91 | 42 | 2 | 97 | 178 | 319 | 409 | 1.7e-07 | 65.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene75963 | 0.84774 | 9.55E-60 | -1.69E-46 |
| MS.gene049271 | MS.gene75963 | 0.814445 | 1.54E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene75963 | 0.805664 | 1.20E-49 | -1.69E-46 |
| MS.gene049404 | MS.gene75963 | 0.824988 | 5.99E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene75963 | 0.816678 | 4.89E-52 | -1.69E-46 |
| MS.gene049452 | MS.gene75963 | 0.801061 | 1.08E-48 | -1.69E-46 |
| MS.gene049567 | MS.gene75963 | 0.802245 | 6.15E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene75963 | 0.816496 | 5.37E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene75963 | 0.815329 | 9.78E-52 | -1.69E-46 |
| MS.gene049821 | MS.gene75963 | 0.800382 | 1.48E-48 | -1.69E-46 |
| MS.gene049824 | MS.gene75963 | 0.813022 | 3.16E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene75963 | 0.838594 | 2.62E-57 | -1.69E-46 |
| MS.gene049850 | MS.gene75963 | 0.813994 | 1.93E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene75963 | 0.8451 | 5.01E-59 | -1.69E-46 |
| MS.gene050026 | MS.gene75963 | 0.851943 | 6.38E-61 | -1.69E-46 |
| MS.gene050031 | MS.gene75963 | 0.803971 | 2.70E-49 | -1.69E-46 |
| MS.gene050043 | MS.gene75963 | 0.806473 | 8.09E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene75963 | 0.803638 | 3.17E-49 | -1.69E-46 |
| MS.gene050201 | MS.gene75963 | 0.828645 | 8.01E-55 | -1.69E-46 |
| MS.gene050273 | MS.gene75963 | 0.807954 | 3.93E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene75963 | 0.829844 | 4.10E-55 | -1.69E-46 |
| MS.gene050442 | MS.gene75963 | 0.819441 | 1.16E-52 | -1.69E-46 |
| MS.gene050559 | MS.gene75963 | 0.829165 | 6.00E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene75963 | 0.825279 | 5.11E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene75963 | 0.818433 | 1.97E-52 | -1.69E-46 |
| MS.gene050719 | MS.gene75963 | 0.80166 | 8.11E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene75963 | 0.815487 | 9.02E-52 | -1.69E-46 |
| MS.gene050759 | MS.gene75963 | 0.808684 | 2.74E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene75963 | 0.807326 | 5.34E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene75963 | 0.803925 | 2.76E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene75963 | 0.816718 | 4.79E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene75963 | 0.840622 | 7.78E-58 | -1.69E-46 |
| MS.gene051279 | MS.gene75963 | 0.834439 | 2.99E-56 | -1.69E-46 |
| MS.gene051574 | MS.gene75963 | 0.804722 | 1.88E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene75963 | 0.829868 | 4.04E-55 | -1.69E-46 |
| MS.gene051700 | MS.gene75963 | 0.802677 | 5.01E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene75963 | 0.849863 | 2.46E-60 | -1.69E-46 |
| MS.gene051720 | MS.gene75963 | 0.84048 | 8.48E-58 | -1.69E-46 |
| MS.gene051744 | MS.gene75963 | 0.832282 | 1.03E-55 | -1.69E-46 |
| MS.gene051957 | MS.gene75963 | 0.816836 | 4.50E-52 | -1.69E-46 |
| MS.gene052190 | MS.gene75963 | 0.842405 | 2.64E-58 | -1.69E-46 |
| MS.gene052208 | MS.gene75963 | 0.822185 | 2.71E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene75963 | 0.840788 | 7.04E-58 | -1.69E-46 |
| MS.gene052358 | MS.gene75963 | -0.809223 | 2.10E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene75963 | 0.802578 | 5.25E-49 | -1.69E-46 |
| MS.gene052488 | MS.gene75963 | 0.816788 | 4.62E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene75963 | 0.800734 | 1.25E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene75963 | 0.873517 | 1.38E-67 | -1.69E-46 |
| MS.gene052582 | MS.gene75963 | 0.870266 | 1.66E-66 | -1.69E-46 |
| MS.gene052584 | MS.gene75963 | 0.872806 | 2.40E-67 | -1.69E-46 |
| MS.gene052586 | MS.gene75963 | 0.83053 | 2.79E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene75963 | 0.857242 | 1.87E-62 | -1.69E-46 |
| MS.gene052829 | MS.gene75963 | 0.800211 | 1.60E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene75963 | 0.804207 | 2.41E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene75963 | 0.814122 | 1.81E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene75963 | 0.814572 | 1.44E-51 | -1.69E-46 |
| MS.gene053054 | MS.gene75963 | -0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene053145 | MS.gene75963 | 0.808095 | 3.67E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene75963 | 0.818946 | 1.50E-52 | -1.69E-46 |
| MS.gene053202 | MS.gene75963 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene053221 | MS.gene75963 | 0.824962 | 6.08E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene75963 | 0.858135 | 1.01E-62 | -1.69E-46 |
| MS.gene053599 | MS.gene75963 | 0.821718 | 3.48E-53 | -1.69E-46 |
| MS.gene05365 | MS.gene75963 | 0.820179 | 7.86E-53 | -1.69E-46 |
| MS.gene053883 | MS.gene75963 | 0.801316 | 9.54E-49 | -1.69E-46 |
| MS.gene053966 | MS.gene75963 | 0.81196 | 5.39E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene75963 | 0.80702 | 6.20E-50 | -1.69E-46 |
| MS.gene053995 | MS.gene75963 | 0.800015 | 1.76E-48 | -1.69E-46 |
| MS.gene05414 | MS.gene75963 | 0.842967 | 1.87E-58 | -1.69E-46 |
| MS.gene05415 | MS.gene75963 | 0.852931 | 3.34E-61 | -1.69E-46 |
| MS.gene054236 | MS.gene75963 | 0.82629 | 2.94E-54 | -1.69E-46 |
| MS.gene05423 | MS.gene75963 | 0.833791 | 4.35E-56 | -1.69E-46 |
| MS.gene054291 | MS.gene75963 | -0.852631 | 4.07E-61 | -1.69E-46 |
| MS.gene054467 | MS.gene75963 | 0.811941 | 5.44E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene75963 | 0.816301 | 5.94E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene75963 | 0.819999 | 8.65E-53 | -1.69E-46 |
| MS.gene054576 | MS.gene75963 | 0.808486 | 3.02E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene75963 | 0.858391 | 8.51E-63 | -1.69E-46 |
| MS.gene054775 | MS.gene75963 | 0.855632 | 5.54E-62 | -1.69E-46 |
| MS.gene054991 | MS.gene75963 | 0.826435 | 2.72E-54 | -1.69E-46 |
| MS.gene055166 | MS.gene75963 | 0.824451 | 8.01E-54 | -1.69E-46 |
| MS.gene055228 | MS.gene75963 | 0.817729 | 2.84E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene75963 | 0.800521 | 1.39E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene75963 | 0.802475 | 5.51E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene75963 | 0.833372 | 5.54E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene75963 | 0.803452 | 3.46E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene75963 | 0.818847 | 1.58E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene75963 | 0.850792 | 1.35E-60 | -1.69E-46 |
| MS.gene055808 | MS.gene75963 | 0.807528 | 4.84E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene75963 | 0.837028 | 6.62E-57 | -1.69E-46 |
| MS.gene055841 | MS.gene75963 | 0.814538 | 1.46E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene75963 | 0.808825 | 2.56E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene75963 | 0.806576 | 7.69E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene75963 | 0.811376 | 7.22E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene75963 | 0.818332 | 2.07E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene75963 | 0.820265 | 7.51E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene75963 | 0.830407 | 2.99E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene75963 | 0.819416 | 1.17E-52 | -1.69E-46 |
| MS.gene056272 | MS.gene75963 | 0.803032 | 4.23E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene75963 | 0.825218 | 5.28E-54 | -1.69E-46 |
| MS.gene056373 | MS.gene75963 | 0.810697 | 1.01E-50 | -1.69E-46 |
| MS.gene056498 | MS.gene75963 | 0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene75963 | 0.817415 | 3.34E-52 | -1.69E-46 |
| MS.gene056595 | MS.gene75963 | 0.805584 | 1.24E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene75963 | 0.803549 | 3.31E-49 | -1.69E-46 |
| MS.gene05690 | MS.gene75963 | 0.816053 | 6.74E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene75963 | 0.824242 | 8.97E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene75963 | 0.802127 | 6.50E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene75963 | 0.840307 | 9.41E-58 | -1.69E-46 |
| MS.gene057208 | MS.gene75963 | 0.830503 | 2.83E-55 | -1.69E-46 |
| MS.gene057314 | MS.gene75963 | 0.818567 | 1.83E-52 | -1.69E-46 |
| MS.gene057414 | MS.gene75963 | 0.832975 | 6.95E-56 | -1.69E-46 |
| MS.gene057444 | MS.gene75963 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene057598 | MS.gene75963 | 0.803771 | 2.97E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene75963 | 0.828396 | 9.20E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene75963 | 0.806989 | 6.29E-50 | -1.69E-46 |
| MS.gene057921 | MS.gene75963 | 0.813132 | 2.99E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene75963 | 0.803018 | 4.26E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene75963 | 0.807815 | 4.20E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene75963 | 0.845367 | 4.25E-59 | -1.69E-46 |
| MS.gene058294 | MS.gene75963 | 0.800677 | 1.29E-48 | -1.69E-46 |
| MS.gene058625 | MS.gene75963 | 0.874157 | 8.41E-68 | -1.69E-46 |
| MS.gene058636 | MS.gene75963 | 0.815625 | 8.40E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene75963 | 0.810551 | 1.09E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene75963 | 0.844205 | 8.73E-59 | -1.69E-46 |
| MS.gene059015 | MS.gene75963 | 0.813793 | 2.14E-51 | -1.69E-46 |
| MS.gene059084 | MS.gene75963 | 0.826349 | 2.85E-54 | -1.69E-46 |
| MS.gene059272 | MS.gene75963 | 0.815657 | 8.26E-52 | -1.69E-46 |
| MS.gene059459 | MS.gene75963 | 0.801106 | 1.05E-48 | -1.69E-46 |
| MS.gene059460 | MS.gene75963 | 0.801932 | 7.13E-49 | -1.69E-46 |
| MS.gene05950 | MS.gene75963 | 0.818031 | 2.42E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene75963 | 0.861922 | 7.30E-64 | -1.69E-46 |
| MS.gene059584 | MS.gene75963 | 0.831657 | 1.47E-55 | -1.69E-46 |
| MS.gene059625 | MS.gene75963 | 0.851728 | 7.35E-61 | -1.69E-46 |
| MS.gene060137 | MS.gene75963 | -0.809628 | 1.72E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene75963 | 0.839975 | 1.15E-57 | -1.69E-46 |
| MS.gene060429 | MS.gene75963 | 0.801034 | 1.09E-48 | -1.69E-46 |
| MS.gene060521 | MS.gene75963 | 0.84511 | 4.98E-59 | -1.69E-46 |
| MS.gene06052 | MS.gene75963 | 0.818103 | 2.33E-52 | -1.69E-46 |
| MS.gene060553 | MS.gene75963 | 0.802113 | 6.54E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene75963 | 0.831274 | 1.83E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene75963 | 0.802391 | 5.74E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene75963 | -0.849077 | 4.07E-60 | -1.69E-46 |
| MS.gene060781 | MS.gene75963 | 0.811002 | 8.71E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene75963 | -0.854061 | 1.58E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75963.t1 | MTR_2g010790 | 42.202 | 109 | 48 | 3 | 79 | 178 | 307 | 409 | 6.46e-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75963.t1 | AT4G10920 | 48.438 | 64 | 30 | 1 | 1 | 64 | 101 | 161 | 1.70e-11 | 60.1 |
| MS.gene75963.t1 | AT4G10920 | 48.438 | 64 | 30 | 1 | 1 | 64 | 101 | 161 | 1.70e-11 | 60.1 |
Find 51 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCGAAATTGATGTGATTT+TGG | 0.193141 | 6.4:+4747429 | MS.gene75963:CDS |
| ATCTATATTTGCCTCTTTCT+TGG | 0.229552 | 6.4:-4747308 | None:intergenic |
| TTTGAATTGTAACTTTCTTT+TGG | 0.278276 | 6.4:-4746353 | None:intergenic |
| GATTTGAAAACGAAAGAAAT+AGG | 0.298954 | 6.4:+4747328 | MS.gene75963:CDS |
| GGATCAGTGGTCCATCTTAA+AGG | 0.299308 | 6.4:+4747198 | MS.gene75963:CDS |
| AGAAGAAGCATATATAAAAC+TGG | 0.300076 | 6.4:+4747237 | MS.gene75963:CDS |
| CAAGAAAGAATATGCTAAAA+TGG | 0.320277 | 6.4:+4747363 | MS.gene75963:CDS |
| GGGACCCTCCCAGTTCTGAC+TGG | 0.366748 | 6.4:-4747267 | None:intergenic |
| AGACAAAATTTGTCTCCATT+AGG | 0.391141 | 6.4:+4746394 | MS.gene75963:CDS |
| GGGAGGGTCCCAGTCAGAAC+TGG | 0.394937 | 6.4:+4747258 | MS.gene75963:CDS |
| TTCGGAGAAGACTGGATTGC+AGG | 0.395516 | 6.4:+4747582 | MS.gene75963:CDS |
| GGATCGTAGACAGCTGATTT+CGG | 0.401576 | 6.4:+4747564 | MS.gene75963:CDS |
| CATTGGAGGAGAGGTGGAAT+AGG | 0.405658 | 6.4:+4747515 | MS.gene75963:CDS |
| TAACCTTCTTCTCGGCAATC+TGG | 0.406973 | 6.4:-4747540 | None:intergenic |
| AGACTGGATTGCAGGCATTT+CGG | 0.409767 | 6.4:+4747590 | MS.gene75963:CDS |
| TGGCACGCTTTGTTTCTGAC+TGG | 0.413267 | 6.4:-4747288 | None:intergenic |
| TGAACATGGACGACGTGAAA+AGG | 0.420395 | 6.4:+4747458 | MS.gene75963:CDS |
| GGCACGCTTTGTTTCTGACT+GGG | 0.423241 | 6.4:-4747287 | None:intergenic |
| ACGATCCATAACCTTCTTCT+CGG | 0.447059 | 6.4:-4747548 | None:intergenic |
| ATATATGCTTCTTCTATGGC+AGG | 0.452325 | 6.4:-4747229 | None:intergenic |
| ATCTGAGAGGATTGCATTGG+AGG | 0.457412 | 6.4:+4747501 | MS.gene75963:CDS |
| GATTGCCGAGAAGAAGGTTA+TGG | 0.458622 | 6.4:+4747543 | MS.gene75963:CDS |
| GAAAAGGGGATTGGAATTGA+TGG | 0.468138 | 6.4:+4747474 | MS.gene75963:CDS |
| AAAACGAAAGAAATAGGTTG+TGG | 0.469430 | 6.4:+4747334 | MS.gene75963:CDS |
| GAACATGGACGACGTGAAAA+GGG | 0.481916 | 6.4:+4747459 | MS.gene75963:CDS |
| CAGCTGATTTCGGAGAAGAC+TGG | 0.483314 | 6.4:+4747574 | MS.gene75963:CDS |
| AGGCATATTTGCCTTTAAGA+TGG | 0.494076 | 6.4:-4747209 | None:intergenic |
| GTTCCAGATTGCCGAGAAGA+AGG | 0.495198 | 6.4:+4747537 | MS.gene75963:CDS |
| AACAAAGCGTGCCAAGAAAG+AGG | 0.509036 | 6.4:+4747297 | MS.gene75963:CDS |
| GGATTGCATTGGAGGAGAGG+TGG | 0.517797 | 6.4:+4747509 | MS.gene75963:CDS |
| GGACGACGTGAAAAGGGGAT+TGG | 0.526254 | 6.4:+4747465 | MS.gene75963:CDS |
| GGACCCTCCCAGTTCTGACT+GGG | 0.529301 | 6.4:-4747266 | None:intergenic |
| AAAAGGGGATTGGAATTGAT+GGG | 0.547783 | 6.4:+4747475 | MS.gene75963:CDS |
| GGAGGGTCCCAGTCAGAACT+GGG | 0.548617 | 6.4:+4747259 | MS.gene75963:CDS |
| AGAGGATTGCATTGGAGGAG+AGG | 0.560680 | 6.4:+4747506 | MS.gene75963:CDS |
| GAAATCTGAGAGGATTGCAT+TGG | 0.563890 | 6.4:+4747498 | MS.gene75963:CDS |
| AAAGAATATGCTAAAATGGA+AGG | 0.575746 | 6.4:+4747367 | MS.gene75963:CDS |
| TTTAGGAATAAGTTTGACTG+TGG | 0.581440 | 6.4:+4747177 | MS.gene75963:intron |
| CAATTCAAAAGCGGAATGAG+TGG | 0.584388 | 6.4:+4746367 | MS.gene75963:CDS |
| AACATGGACGACGTGAAAAG+GGG | 0.602059 | 6.4:+4747460 | MS.gene75963:CDS |
| TAAGTTTGACTGTGGATCAG+TGG | 0.608779 | 6.4:+4747185 | MS.gene75963:CDS |
| GAATATGCTAAAATGGAAGG+TGG | 0.613411 | 6.4:+4747370 | MS.gene75963:CDS |
| GGTCCCAGTCAGAACTGGGA+GGG | 0.621909 | 6.4:+4747263 | MS.gene75963:CDS |
| GGGTCCCAGTCAGAACTGGG+AGG | 0.629574 | 6.4:+4747262 | MS.gene75963:CDS |
| AGAAAGTTACAATTCAAAAG+CGG | 0.632985 | 6.4:+4746358 | MS.gene75963:CDS |
| GAAGAAGCATATATAAAACT+GGG | 0.640059 | 6.4:+4747238 | MS.gene75963:CDS |
| GTGGAATGTCCTTCTAACCC+AGG | 0.643475 | 6.4:+4746438 | MS.gene75963:CDS |
| AAGCATATATAAAACTGGGA+GGG | 0.653918 | 6.4:+4747242 | MS.gene75963:CDS |
| GAAGCATATATAAAACTGGG+AGG | 0.661078 | 6.4:+4747241 | MS.gene75963:CDS |
| AAAGGGGATTGGAATTGATG+GGG | 0.671071 | 6.4:+4747476 | MS.gene75963:CDS |
| AATTGATGGGGAAATCTGAG+AGG | 0.674174 | 6.4:+4747488 | MS.gene75963:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTAATTTCAAAAGT+AGG | + | chr6.4:4747043-4747062 | MS.gene75963:intron | 15.0% |
| !!! | AAAAAAAAAAGTACAACTTT+AGG | + | chr6.4:4746753-4746772 | MS.gene75963:intron | 15.0% |
| !! | TAAAATTACAGATAAAGTCA+AGG | - | chr6.4:4747088-4747107 | None:intergenic | 20.0% |
| !! | TTTAAAAACAAACTTCCTAA+TGG | - | chr6.4:4746412-4746431 | None:intergenic | 20.0% |
| !!! | TTTGAATTGTAACTTTCTTT+TGG | - | chr6.4:4746356-4746375 | None:intergenic | 20.0% |
| !!! | TTTTATATATGCTTCTTCTA+TGG | - | chr6.4:4747236-4747255 | None:intergenic | 20.0% |
| ! | AAAATTTAAGTACACATCCT+TGG | + | chr6.4:4746983-4747002 | MS.gene75963:intron | 25.0% |
| ! | AAAGAATATGCTAAAATGGA+AGG | + | chr6.4:4747367-4747386 | MS.gene75963:CDS | 25.0% |
| ! | AGAAAACAATAAAACCCAAA+TGG | - | chr6.4:4746626-4746645 | None:intergenic | 25.0% |
| ! | AGAAAGTTACAATTCAAAAG+CGG | + | chr6.4:4746358-4746377 | MS.gene75963:CDS | 25.0% |
| ! | CAAGAAAGAATATGCTAAAA+TGG | + | chr6.4:4747363-4747382 | MS.gene75963:CDS | 25.0% |
| ! | GAAAACAATAAAACCCAAAT+GGG | - | chr6.4:4746625-4746644 | None:intergenic | 25.0% |
| ! | GATTTGAAAACGAAAGAAAT+AGG | + | chr6.4:4747328-4747347 | MS.gene75963:CDS | 25.0% |
| ! | TATAACACAAAATCAGAGTT+AGG | + | chr6.4:4746954-4746973 | MS.gene75963:intron | 25.0% |
| ! | TTCTTAAGATTGTGTTTGTT+AGG | - | chr6.4:4746918-4746937 | None:intergenic | 25.0% |
| !! | AGAAGAAGCATATATAAAAC+TGG | + | chr6.4:4747237-4747256 | MS.gene75963:CDS | 25.0% |
| !! | ATTCGAAATTGATGTGATTT+TGG | + | chr6.4:4747429-4747448 | MS.gene75963:CDS | 25.0% |
| !! | GAAGAAGCATATATAAAACT+GGG | + | chr6.4:4747238-4747257 | MS.gene75963:CDS | 25.0% |
| !! | TAAAAAGTATGCAAACTTTG+AGG | - | chr6.4:4746577-4746596 | None:intergenic | 25.0% |
| !!! | ACTTTCTTTTTCAAGTAACA+TGG | - | chr6.4:4746554-4746573 | None:intergenic | 25.0% |
| !!! | AGGAAGTTTGTTTTTAAACA+CGG | + | chr6.4:4746414-4746433 | MS.gene75963:CDS | 25.0% |
| !!! | CATTTTAGCATATTCTTTCT+TGG | - | chr6.4:4747365-4747384 | None:intergenic | 25.0% |
| !!! | CTTTTTTTATCTTGTCACTA+AGG | - | chr6.4:4747030-4747049 | None:intergenic | 25.0% |
| !!! | TTTCTATTTTTCTGCGTTTT+AGG | + | chr6.4:4747160-4747179 | MS.gene75963:intron | 25.0% |
| AAAACGAAAGAAATAGGTTG+TGG | + | chr6.4:4747334-4747353 | MS.gene75963:CDS | 30.0% | |
| AAGCATATATAAAACTGGGA+GGG | + | chr6.4:4747242-4747261 | MS.gene75963:CDS | 30.0% | |
| ACAAAACTAAAATGACATCG+AGG | - | chr6.4:4746495-4746514 | None:intergenic | 30.0% | |
| AGACAAAATTTGTCTCCATT+AGG | + | chr6.4:4746394-4746413 | MS.gene75963:CDS | 30.0% | |
| AGTAACATGGATAAACTTTG+AGG | - | chr6.4:4746541-4746560 | None:intergenic | 30.0% | |
| ATCTATATTTGCCTCTTTCT+TGG | - | chr6.4:4747311-4747330 | None:intergenic | 30.0% | |
| CAATCTTACCATATGTACTA+TGG | - | chr6.4:4746866-4746885 | None:intergenic | 30.0% | |
| GCAAATAACCATAGTACATA+TGG | + | chr6.4:4746855-4746874 | MS.gene75963:intron | 30.0% | |
| GTGTTATATTTGAGTGTTTC+AGG | - | chr6.4:4746942-4746961 | None:intergenic | 30.0% | |
| TTTAGGAATAAGTTTGACTG+TGG | + | chr6.4:4747177-4747196 | MS.gene75963:intron | 30.0% | |
| ! | AAAGCACAATACATTTTACC+TGG | - | chr6.4:4746459-4746478 | None:intergenic | 30.0% |
| ! | AAGCACAATACATTTTACCT+GGG | - | chr6.4:4746458-4746477 | None:intergenic | 30.0% |
| ! | GTATGCATTTTTAATCTCCA+AGG | - | chr6.4:4747003-4747022 | None:intergenic | 30.0% |
| ! | TTAAGATTGTGTTTGTTAGG+AGG | - | chr6.4:4746915-4746934 | None:intergenic | 30.0% |
| !!! | TGGGTTTTATTGTTTTCTGT+TGG | + | chr6.4:4746628-4746647 | MS.gene75963:intron | 30.0% |
| AAAAGGGGATTGGAATTGAT+GGG | + | chr6.4:4747475-4747494 | MS.gene75963:CDS | 35.0% | |
| AGACAACATGCACCATATAT+AGG | - | chr6.4:4746659-4746678 | None:intergenic | 35.0% | |
| AGATAAAGTCAAGGATGTGT+AGG | - | chr6.4:4747079-4747098 | None:intergenic | 35.0% | |
| ATATATGCTTCTTCTATGGC+AGG | - | chr6.4:4747232-4747251 | None:intergenic | 35.0% | |
| GAAGCATATATAAAACTGGG+AGG | + | chr6.4:4747241-4747260 | MS.gene75963:CDS | 35.0% | |
| GAATATGCTAAAATGGAAGG+TGG | + | chr6.4:4747370-4747389 | MS.gene75963:CDS | 35.0% | |
| GTCATCTTTGATTCCCATTT+GGG | + | chr6.4:4746609-4746628 | MS.gene75963:intron | 35.0% | |
| TGTCATCTTTGATTCCCATT+TGG | + | chr6.4:4746608-4746627 | MS.gene75963:intron | 35.0% | |
| ! | AGGCATATTTGCCTTTAAGA+TGG | - | chr6.4:4747212-4747231 | None:intergenic | 35.0% |
| ! | CGAAATTGATGTGATTTTGG+CGG | + | chr6.4:4747432-4747451 | MS.gene75963:CDS | 35.0% |
| !! | AAATTGATGTGATTTTGGCG+GGG | + | chr6.4:4747434-4747453 | MS.gene75963:CDS | 35.0% |
| !! | GAAATTGATGTGATTTTGGC+GGG | + | chr6.4:4747433-4747452 | MS.gene75963:CDS | 35.0% |
| !! | TACATTTTACCTGGGTTAGA+AGG | - | chr6.4:4746450-4746469 | None:intergenic | 35.0% |
| !!! | GTTTGTTTTTAAACACGGAG+TGG | + | chr6.4:4746419-4746438 | MS.gene75963:CDS | 35.0% |
| AAAGGGGATTGGAATTGATG+GGG | + | chr6.4:4747476-4747495 | MS.gene75963:CDS | 40.0% | |
| AATTGATGGGGAAATCTGAG+AGG | + | chr6.4:4747488-4747507 | MS.gene75963:CDS | 40.0% | |
| ACGATCCATAACCTTCTTCT+CGG | - | chr6.4:4747551-4747570 | None:intergenic | 40.0% | |
| CAACATGCACCATATATAGG+AGG | - | chr6.4:4746656-4746675 | None:intergenic | 40.0% | |
| CAATTCAAAAGCGGAATGAG+TGG | + | chr6.4:4746367-4746386 | MS.gene75963:CDS | 40.0% | |
| GAAAAGGGGATTGGAATTGA+TGG | + | chr6.4:4747474-4747493 | MS.gene75963:CDS | 40.0% | |
| GAAATCTGAGAGGATTGCAT+TGG | + | chr6.4:4747498-4747517 | MS.gene75963:CDS | 40.0% | |
| TAAGTTTGACTGTGGATCAG+TGG | + | chr6.4:4747185-4747204 | MS.gene75963:CDS | 40.0% | |
| AACAAAGCGTGCCAAGAAAG+AGG | + | chr6.4:4747297-4747316 | MS.gene75963:CDS | 45.0% | |
| AACATGGACGACGTGAAAAG+GGG | + | chr6.4:4747460-4747479 | MS.gene75963:CDS | 45.0% | |
| AGACTGGATTGCAGGCATTT+CGG | + | chr6.4:4747590-4747609 | MS.gene75963:CDS | 45.0% | |
| ATCTGAGAGGATTGCATTGG+AGG | + | chr6.4:4747501-4747520 | MS.gene75963:CDS | 45.0% | |
| GAACATGGACGACGTGAAAA+GGG | + | chr6.4:4747459-4747478 | MS.gene75963:CDS | 45.0% | |
| GATTGCCGAGAAGAAGGTTA+TGG | + | chr6.4:4747543-4747562 | MS.gene75963:CDS | 45.0% | |
| GGATCAGTGGTCCATCTTAA+AGG | + | chr6.4:4747198-4747217 | MS.gene75963:CDS | 45.0% | |
| TAACCTTCTTCTCGGCAATC+TGG | - | chr6.4:4747543-4747562 | None:intergenic | 45.0% | |
| TGAACATGGACGACGTGAAA+AGG | + | chr6.4:4747458-4747477 | MS.gene75963:CDS | 45.0% | |
| ! | CTGTTGGTGCCTCCTATATA+TGG | + | chr6.4:4746644-4746663 | MS.gene75963:intron | 45.0% |
| ! | GGATCGTAGACAGCTGATTT+CGG | + | chr6.4:4747564-4747583 | MS.gene75963:CDS | 45.0% |
| AGAGGATTGCATTGGAGGAG+AGG | + | chr6.4:4747506-4747525 | MS.gene75963:CDS | 50.0% | |
| CATTGGAGGAGAGGTGGAAT+AGG | + | chr6.4:4747515-4747534 | MS.gene75963:CDS | 50.0% | |
| GTGGAATGTCCTTCTAACCC+AGG | + | chr6.4:4746438-4746457 | MS.gene75963:CDS | 50.0% | |
| GTTCCAGATTGCCGAGAAGA+AGG | + | chr6.4:4747537-4747556 | MS.gene75963:CDS | 50.0% | |
| TTCGGAGAAGACTGGATTGC+AGG | + | chr6.4:4747582-4747601 | MS.gene75963:CDS | 50.0% | |
| ! | CAGCTGATTTCGGAGAAGAC+TGG | + | chr6.4:4747574-4747593 | MS.gene75963:CDS | 50.0% |
| !! | GGCACGCTTTGTTTCTGACT+GGG | - | chr6.4:4747290-4747309 | None:intergenic | 50.0% |
| !! | TGGCACGCTTTGTTTCTGAC+TGG | - | chr6.4:4747291-4747310 | None:intergenic | 50.0% |
| GGACGACGTGAAAAGGGGAT+TGG | + | chr6.4:4747465-4747484 | MS.gene75963:CDS | 55.0% | |
| GGATTGCATTGGAGGAGAGG+TGG | + | chr6.4:4747509-4747528 | MS.gene75963:CDS | 55.0% | |
| !! | GATTTTGGCGGGGCTGAACA+TGG | + | chr6.4:4747444-4747463 | MS.gene75963:CDS | 55.0% |
| GGAGGGTCCCAGTCAGAACT+GGG | + | chr6.4:4747259-4747278 | MS.gene75963:CDS | 60.0% | |
| GGTCCCAGTCAGAACTGGGA+GGG | + | chr6.4:4747263-4747282 | MS.gene75963:CDS | 60.0% | |
| ! | GGACCCTCCCAGTTCTGACT+GGG | - | chr6.4:4747269-4747288 | None:intergenic | 60.0% |
| GGGAGGGTCCCAGTCAGAAC+TGG | + | chr6.4:4747258-4747277 | MS.gene75963:CDS | 65.0% | |
| GGGTCCCAGTCAGAACTGGG+AGG | + | chr6.4:4747262-4747281 | MS.gene75963:CDS | 65.0% | |
| ! | GGGACCCTCCCAGTTCTGAC+TGG | - | chr6.4:4747270-4747289 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 4746345 | 4747616 | 4746345 | ID=MS.gene75963 |
| chr6.4 | mRNA | 4746345 | 4747616 | 4746345 | ID=MS.gene75963.t1;Parent=MS.gene75963 |
| chr6.4 | exon | 4746345 | 4746459 | 4746345 | ID=MS.gene75963.t1.exon1;Parent=MS.gene75963.t1 |
| chr6.4 | CDS | 4746345 | 4746459 | 4746345 | ID=cds.MS.gene75963.t1;Parent=MS.gene75963.t1 |
| chr6.4 | exon | 4747182 | 4747616 | 4747182 | ID=MS.gene75963.t1.exon2;Parent=MS.gene75963.t1 |
| chr6.4 | CDS | 4747182 | 4747616 | 4747182 | ID=cds.MS.gene75963.t1;Parent=MS.gene75963.t1 |
| Gene Sequence |
| Protein sequence |
