AlfalfaGEDB | MS.gene59540

M. sativa cultivar XinJiangDaYe / MS.gene59540

KEGG: K06268
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35 PF13499.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59540.t1	XP_024627594.1	91.9	236	18	1	1	236	1	235	4.40E-112	414.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59540.t1	O81223	60.2	221	82	1	17	237	3	217	1.8e-69	263.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59540.t1	G8A2Y4	91.9	236	18	1	1	236	1	235	3.2e-112	414.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene59540	0.838713	2.44E-57	-1.69E-46
MS.gene04919	MS.gene59540	0.822585	2.19E-53	-1.69E-46
MS.gene04920	MS.gene59540	0.846558	2.01E-59	-1.69E-46
MS.gene049254	MS.gene59540	0.862561	4.64E-64	-1.69E-46
MS.gene049283	MS.gene59540	0.848658	5.33E-60	-1.69E-46
MS.gene049293	MS.gene59540	-0.818584	1.82E-52	-1.69E-46
MS.gene049350	MS.gene59540	0.80691	6.54E-50	-1.69E-46
MS.gene049419	MS.gene59540	-0.820052	8.41E-53	-1.69E-46
MS.gene049499	MS.gene59540	0.862476	4.93E-64	-1.69E-46
MS.gene049652	MS.gene59540	0.804457	2.14E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59540.t1	MTR_8g027620	97.034	236	6	1	1	236	1	235	7.22e-168	465
MS.gene59540.t1	MTR_3g091440	68.878	196	61	0	40	235	17	212	2.71e-95	278
MS.gene59540.t1	MTR_3g091440	66.316	190	64	0	46	235	24	213	2.64e-88	261
MS.gene59540.t1	MTR_8g099575	61.538	195	75	0	40	234	16	210	1.82e-85	253
MS.gene59540.t1	MTR_4g099210	56.923	195	84	0	40	234	16	210	9.29e-79	236
MS.gene59540.t1	MTR_3g060730	54.146	205	92	1	33	235	22	226	1.27e-76	231
MS.gene59540.t1	MTR_4g134250	55.440	193	86	0	43	235	35	227	1.73e-76	231
MS.gene59540.t1	MTR_1g016430	57.436	195	83	0	38	232	53	247	2.20e-76	231
MS.gene59540.t1	MTR_5g096420	55.612	196	87	0	40	235	31	226	4.57e-76	229
MS.gene59540.t1	MTR_3g060730	55.435	184	82	0	52	235	5	188	7.16e-73	220

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59540.t1	AT5G24270	60.181	221	82	1	17	237	3	217	1.62e-93	274
MS.gene59540.t1	AT5G24270	60.181	221	82	1	17	237	3	217	1.62e-93	274
MS.gene59540.t1	AT5G24270	60.181	221	82	1	17	237	3	217	1.62e-93	274
MS.gene59540.t1	AT5G24270	63.492	189	69	0	49	237	4	192	1.28e-86	255
MS.gene59540.t1	AT1G64480	60.000	195	78	0	40	234	20	214	5.95e-79	236
MS.gene59540.t1	AT1G64480	60.000	195	78	0	40	234	20	214	5.95e-79	236
MS.gene59540.t1	AT1G64480	60.000	195	78	0	40	234	20	214	5.95e-79	236
MS.gene59540.t1	AT5G24270	62.805	164	61	0	74	237	7	170	1.23e-75	226
MS.gene59540.t1	AT5G47100	56.842	190	82	0	45	234	21	210	1.43e-75	228
MS.gene59540.t1	AT4G33000	58.247	194	81	0	41	234	53	246	1.44e-75	229

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Find 32 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTAGCAGAAAATATTA+TGG	0.327214	8.4:+68733605	MS.gene59540:CDS
AAACAAACGCAACCTCTTTG+TGG	0.437485	8.4:+68734584	MS.gene59540:CDS
AAACTACAAGTGTGATTCTT+CGG	0.432593	8.4:-68735648	None:intergenic
AAATTTACAGTTGAAGGATA+TGG	0.379306	8.4:+68735145	MS.gene59540:intron
AACGCAACCTCTTTGTGGAT+AGG	0.564160	8.4:+68734589	MS.gene59540:CDS
ACGCAACCTCTTTGTGGATA+GGG	0.528181	8.4:+68734590	MS.gene59540:CDS
AGCAGTTACTGTGAGAGAGG+TGG	0.654761	8.4:+68734325	MS.gene59540:intron
ATGGATGGATCCTGTGAAGT+TGG	0.558937	8.4:+68733624	MS.gene59540:CDS
CACAACGGCCATATAGAGTT+TGG	0.395021	8.4:+68734753	MS.gene59540:CDS
CATTTAGATTATATGACTTG+AGG	0.558619	8.4:+68735016	MS.gene59540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCATTTACTATTCATTTCG+AGG	-	chr8.4:68735506-68735525	None:intergenic	30.0%
ACCTCGAAATGAATAGTAAA+TGG	+	chr8.4:68735502-68735521	MS.gene59540:intron	30.0%
AGCACAGTTATTGTGAAAAA+TGG	+	chr8.4:68734377-68734396	MS.gene59540:CDS	30.0%
ATAATCACTTCTGCTACAAT+CGG	+	chr8.4:68734181-68734200	MS.gene59540:intron	30.0%
ATTAAAACACACAAGTGGAA+AGG	-	chr8.4:68734246-68734265	None:intergenic	30.0%
GTACACTATTCATATGCAAT+TGG	-	chr8.4:68734277-68734296	None:intergenic	30.0%
GTAGCAGAAAATATTATGGA+TGG	+	chr8.4:68733609-68733628	MS.gene59540:CDS	30.0%
TAAATTCGCCAAACTCTATA+TGG	-	chr8.4:68734764-68734783	None:intergenic	30.0%
TGTATATATGATCAACATCC+TGG	-	chr8.4:68734893-68734912	None:intergenic	30.0%
AATCCAAAAAGCCCTTAAGA+TGG	-	chr8.4:68733975-68733994	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	68733588	68735667	68733588	ID=MS.gene59540
chr8.4	mRNA	68733588	68735667	68733588	ID=MS.gene59540.t1;Parent=MS.gene59540
chr8.4	exon	68733588	68733738	68733588	ID=MS.gene59540.t1.exon1;Parent=MS.gene59540.t1
chr8.4	CDS	68733588	68733738	68733588	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68734330	68734412	68734330	ID=MS.gene59540.t1.exon2;Parent=MS.gene59540.t1
chr8.4	CDS	68734330	68734412	68734330	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68734552	68734611	68734552	ID=MS.gene59540.t1.exon3;Parent=MS.gene59540.t1
chr8.4	CDS	68734552	68734611	68734552	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68734729	68734837	68734729	ID=MS.gene59540.t1.exon4;Parent=MS.gene59540.t1
chr8.4	CDS	68734729	68734837	68734729	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68735013	68735065	68735013	ID=MS.gene59540.t1.exon5;Parent=MS.gene59540.t1
chr8.4	CDS	68735013	68735065	68735013	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68735155	68735235	68735155	ID=MS.gene59540.t1.exon6;Parent=MS.gene59540.t1
chr8.4	CDS	68735155	68735235	68735155	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68735327	68735439	68735327	ID=MS.gene59540.t1.exon7;Parent=MS.gene59540.t1
chr8.4	CDS	68735327	68735439	68735327	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1
chr8.4	exon	68735604	68735667	68735604	ID=MS.gene59540.t1.exon8;Parent=MS.gene59540.t1
chr8.4	CDS	68735604	68735667	68735604	ID=cds.MS.gene59540.t1;Parent=MS.gene59540.t1

Gene Sequence

>MS.gene59540

ATGGGTTGCAGTAGCTCAAAAGTAGCAGAAAATATTATGGATGGATCCTGTGAAGTTGGCAAAGATAAGAATCTACCTTCAGAAGTAAAAGAAAATGAGCTTAATAATTCTTGTTCTGAAGATTTTTCTATCCTTGCTTCTGAAACACCATGTAAGTAAGAAAGCTTCCTTTTCAAGTTTTTTTTTTTTGCCTTGGAAAGTTAAATTGTCTTGACTAATTAATTAATCTTATAATCTGTAAAATTATAGGTAGATACGTGATTTAGATAATTTCATGGACATAGCAAGAGCAATATACTACTTTGATTTAGTGGTCAAAATCCTCTTTTTTACACTTAATATATTAATTAGTTATTGCTTAAAAAAGGGGTTGCATAGTGCATACCATCTTAAGGGCTTTTTGGATTGACTTCTACACTTCTGAAACTATTTGAGAGAATTTATAGAAACAACTTATGACATGTCCGCGAGCGTTTTAAACTTATTTTCATAAGTTCTACAGGATAGCTTATGAAAACAGATTATGGCTTACATGAAAACAATTTTATTTTATCTTTTGTTATAGAAATAACTTATACATAAGCACTTATTTGATAATCACTTCTGCTACAATCGGTTAATAAGTTGTTTATCCAAACAGAACCTGAAATTGGTTCCTTTCCACTTGTGTGTTTTAATTTATGCAACCAATTGCATATGAATAGTGTACATTGTTTTGAAGATCTTTTTTTGTACCTAGCAGTTACTGTGAGAGAGGTGGAAATCTTGAAGGAGTTGTACAAAAAATTAAGCACAGTTATTGTGAAAAATGGACTTATTCAAAAGGTAGTTTTTTAAATTCGACGTAGTTCAGTGAAACTTGAATCTCTTGTTCGATTGGAGAATCAGTATTTTTGTACTTCTTTAGATGCATTTCTTGCAAATAATTTATAATAATTGATTTGTGTATTGTGTATATTAACAGGAAGAGCTTCACTTTGCTCTTTTTAGAAACAGAAACAAACGCAACCTCTTTGTGGATAGGGTATGCATGTTGATCTAATTTTTGATGTTCTTACACATTCTTAATATGTACTATATGCACATGTTGATTATGAAAATAGCTTTTCAAGTATAATCGTTTGATCTTCTTTCCATGCAGATGTTTGATCTTTTCGATGTCAATCACAACGGCCATATAGAGTTTGGCGAATTTATCAGATCTTTAGGCATCTTTCATCCTAAAGCTCCTCAAGCAGACAAAATAAAATGTAAGAAATATAAGTCATTGTACATAAGCAATTTAGTCGTTTGTTTACCATTCCAGGATGTTGATCATATATACAATCATATAAATTGGATCACATCGTATTCATTAGCATATTAAATTTCAAGATGATATTGTTAAGTATATGAATACTCACCACGTAAAATTATTCTTCGCAGATGCATTTAGATTATATGACTTGAGGCATACTGGTTACATTGAGCGCGAAGAGGTAAATACTCCTGTGGTCTTTCATAGGATATATTTGTATAATTCTTGTGACAATTGTTTTAATGTTTTGGTTCTTACGAAAATTTACAGTTGAAGGATATGGTGTTGATGATTTTGGTTGAATCTGATTTGGTTCTCTCTGATGATGTGGTTGAAACAATTGTGGACAAGGTTTGTTATCATATGAGACTCTAAATAAAATCATCACTTTATAAATTTTATATGGAAAGTTTCTTATATATATTTTGGGGGGTGTTAACAGACATTTCTAGAAACAGACACGAAAGGTGACGGAAAAATTGACTTAGAAGAATGGAGTGAATATGTTGGAAAGAATCCATCATTACTCAAGAACATGACTCTACCATATCTAATGTAAGTTTTAAGTTTTATTAAATGATACATCCATTCATTGCATTATGATGTTTTTTAAAATGACCTCGAAATGAATAGTAAATGGTCTTCTTTTGTTTTTGGTATCGTTGCGTTATTCCAAGTGACACAGTTGTTTATACATCACTTTTTGATTCTAATTTCAGGGACATAACTCTAGCATTTCCCAGTTTTATGCTGAACGCAGAAACCGAAGAATCACACTTGTAG

Protein sequence

>MS.gene59540.t1

MGCSSSKVAENIMDGSCEVGKDKNLPSEVKENELNNSCSEDFSILASETPFTVREVEILKELYKKLSTVIVKNGLIQKEELHFALFRNRNKRNLFVDRMFDLFDVNHNGHIEFGEFIRSLGIFHPKAPQADKIKYAFRLYDLRHTGYIEREELKDMVLMILVESDLVLSDDVVETIVDKTFLETDTKGDGKIDLEEWSEYVGKNPSLLKNMTLPYLMDITLAFPSFMLNAETEESHL