Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79568.t1 | XP_003621252.1 | 91.1 | 202 | 18 | 0 | 1 | 202 | 1 | 202 | 1.70E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79568.t1 | Q93Z27 | 37.6 | 210 | 117 | 5 | 1 | 202 | 1 | 204 | 3.0e-25 | 116.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79568.t1 | G7L207 | 91.1 | 202 | 18 | 0 | 1 | 202 | 1 | 202 | 1.2e-83 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene79568 | 0.801814 | 7.54E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene79568 | 0.827713 | 1.34E-54 | -1.69E-46 |
| MS.gene050031 | MS.gene79568 | 0.822205 | 2.68E-53 | -1.69E-46 |
| MS.gene050043 | MS.gene79568 | 0.808275 | 3.36E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene79568 | 0.806465 | 8.12E-50 | -1.69E-46 |
| MS.gene051832 | MS.gene79568 | 0.812845 | 3.45E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene79568 | 0.821179 | 4.63E-53 | -1.69E-46 |
| MS.gene052586 | MS.gene79568 | 0.8143 | 1.65E-51 | -1.69E-46 |
| MS.gene052685 | MS.gene79568 | 0.842839 | 2.02E-58 | -1.69E-46 |
| MS.gene053094 | MS.gene79568 | 0.811323 | 7.41E-51 | -1.69E-46 |
| MS.gene053298 | MS.gene79568 | 0.808005 | 3.83E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene79568 | 0.85707 | 2.10E-62 | -1.69E-46 |
| MS.gene05410 | MS.gene79568 | 0.820964 | 5.19E-53 | -1.69E-46 |
| MS.gene054222 | MS.gene79568 | 0.82257 | 2.21E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene79568 | 0.806314 | 8.73E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene79568 | 0.808097 | 3.66E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene79568 | 0.837506 | 4.99E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene79568 | 0.835783 | 1.37E-56 | -1.69E-46 |
| MS.gene055226 | MS.gene79568 | 0.811384 | 7.19E-51 | -1.69E-46 |
| MS.gene056444 | MS.gene79568 | 0.828762 | 7.51E-55 | -1.69E-46 |
| MS.gene056677 | MS.gene79568 | 0.807171 | 5.76E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene79568 | 0.804598 | 2.00E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene79568 | 0.810287 | 1.24E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene79568 | 0.820425 | 6.91E-53 | -1.69E-46 |
| MS.gene059774 | MS.gene79568 | 0.808319 | 3.28E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene79568 | -0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene060012 | MS.gene79568 | 0.80277 | 4.79E-49 | -1.69E-46 |
| MS.gene060257 | MS.gene79568 | 0.817366 | 3.42E-52 | -1.69E-46 |
| MS.gene060259 | MS.gene79568 | 0.807533 | 4.83E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene79568 | 0.839615 | 1.43E-57 | -1.69E-46 |
| MS.gene060413 | MS.gene79568 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene060697 | MS.gene79568 | 0.804955 | 1.68E-49 | -1.69E-46 |
| MS.gene060840 | MS.gene79568 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene061047 | MS.gene79568 | 0.859593 | 3.72E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79568.t1 | MTR_7g011010 | 91.089 | 202 | 18 | 0 | 1 | 202 | 1 | 202 | 5.27e-123 | 346 |
| MS.gene79568.t1 | MTR_5g025690 | 82.524 | 206 | 26 | 2 | 1 | 202 | 1 | 200 | 2.49e-113 | 322 |
| MS.gene79568.t1 | MTR_4g127560 | 67.213 | 183 | 58 | 2 | 22 | 202 | 16 | 198 | 6.62e-74 | 221 |
| MS.gene79568.t1 | MTR_2g023310 | 84.615 | 52 | 8 | 0 | 84 | 135 | 46 | 97 | 6.21e-23 | 88.6 |
| MS.gene79568.t1 | MTR_4g082050 | 46.296 | 108 | 55 | 2 | 91 | 198 | 35 | 139 | 1.53e-19 | 81.3 |
| MS.gene79568.t1 | MTR_7g074240 | 50.769 | 65 | 32 | 0 | 133 | 197 | 106 | 170 | 5.00e-13 | 64.7 |
| MS.gene79568.t1 | MTR_4g115170 | 44.595 | 74 | 41 | 0 | 126 | 199 | 145 | 218 | 1.18e-12 | 64.7 |
| MS.gene79568.t1 | MTR_2g098890 | 27.041 | 196 | 118 | 6 | 25 | 198 | 11 | 203 | 1.80e-11 | 61.2 |
| MS.gene79568.t1 | MTR_8g107110 | 44.118 | 68 | 36 | 1 | 132 | 199 | 94 | 159 | 6.04e-11 | 58.9 |
| MS.gene79568.t1 | MTR_5g017550 | 44.578 | 83 | 42 | 2 | 119 | 199 | 103 | 183 | 9.19e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79568.t1 | AT5G39670 | 43.721 | 215 | 97 | 6 | 1 | 202 | 1 | 204 | 7.63e-47 | 153 |
| MS.gene79568.t1 | AT3G29000 | 63.303 | 109 | 38 | 1 | 91 | 199 | 85 | 191 | 4.28e-41 | 138 |
| MS.gene79568.t1 | AT4G20780 | 54.930 | 71 | 29 | 1 | 127 | 197 | 116 | 183 | 3.35e-15 | 70.9 |
| MS.gene79568.t1 | AT3G47480 | 53.030 | 66 | 30 | 1 | 133 | 198 | 115 | 179 | 3.59e-15 | 70.9 |
| MS.gene79568.t1 | AT5G44460 | 49.296 | 71 | 33 | 1 | 129 | 199 | 109 | 176 | 1.05e-14 | 69.3 |
| MS.gene79568.t1 | AT2G43290 | 31.538 | 130 | 70 | 2 | 87 | 198 | 79 | 207 | 7.79e-14 | 67.8 |
| MS.gene79568.t1 | AT3G59440 | 26.768 | 198 | 106 | 6 | 25 | 199 | 7 | 188 | 6.19e-12 | 62.4 |
| MS.gene79568.t1 | AT1G05990 | 44.615 | 65 | 36 | 0 | 135 | 199 | 80 | 144 | 7.33e-12 | 61.2 |
| MS.gene79568.t1 | AT4G03290 | 43.750 | 64 | 36 | 0 | 135 | 198 | 82 | 145 | 4.55e-11 | 59.3 |
Find 24 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGAGTTATGTGTATTT+TGG | 0.154565 | 7.3:-87073134 | MS.gene79568:CDS |
| CATTGAATTTGTAAAGATTA+TGG | 0.160380 | 7.3:-87073029 | MS.gene79568:CDS |
| TGAAGCGGTGATGGCGAAAA+TGG | 0.258329 | 7.3:-87073317 | MS.gene79568:CDS |
| CTAAGTCACCGGAACGAAAT+TGG | 0.286863 | 7.3:+87073437 | None:intergenic |
| GAGAAAGTGGAGAGATTAAG+AGG | 0.304916 | 7.3:-87073348 | MS.gene79568:CDS |
| GAAAGCAACTCAACTTTGTT+TGG | 0.330887 | 7.3:-87073541 | MS.gene79568:CDS |
| CTTCTCTGCCAATTTCGTTC+CGG | 0.341101 | 7.3:-87073445 | MS.gene79568:CDS |
| TTGTAAAGATTATGGAAAAC+AGG | 0.351343 | 7.3:-87073021 | MS.gene79568:CDS |
| TGATGAGAATGAGCCAAGTT+TGG | 0.401446 | 7.3:-87073224 | MS.gene79568:CDS |
| ATCTTCACTTCCTCCAAACT+TGG | 0.413419 | 7.3:+87073211 | None:intergenic |
| TTCCGGTGACTTAGAAGTAA+AGG | 0.466313 | 7.3:-87073428 | MS.gene79568:CDS |
| TCCGGTGACTTAGAAGTAAA+GGG | 0.483748 | 7.3:-87073427 | MS.gene79568:CDS |
| AAGTTTGGAGGAAGTGAAGA+TGG | 0.496634 | 7.3:-87073209 | MS.gene79568:CDS |
| CGGAACGAAATTGGCAGAGA+AGG | 0.496924 | 7.3:+87073446 | None:intergenic |
| TCCCTTTACTTCTAAGTCAC+CGG | 0.500276 | 7.3:+87073426 | None:intergenic |
| GAAGCGGTGATGGCGAAAAT+GGG | 0.504720 | 7.3:-87073316 | MS.gene79568:CDS |
| TCGAATGTAAGCGGAGAAAG+TGG | 0.508948 | 7.3:-87073361 | MS.gene79568:CDS |
| TGAGAATGAGCCAAGTTTGG+AGG | 0.510803 | 7.3:-87073221 | MS.gene79568:CDS |
| GGATGAAGTTGAAGCGGTGA+TGG | 0.511527 | 7.3:-87073326 | MS.gene79568:CDS |
| CTGCAATAAGATAAACAAGT+TGG | 0.540643 | 7.3:+87073587 | None:intergenic |
| AGAAAGTGGAGAGATTAAGA+GGG | 0.546580 | 7.3:-87073347 | MS.gene79568:CDS |
| TAAGAGGGATGAAGTTGAAG+CGG | 0.562793 | 7.3:-87073332 | MS.gene79568:CDS |
| ACTTGTTTATCTTATTGCAG+AGG | 0.590641 | 7.3:-87073584 | MS.gene79568:CDS |
| AGCAGAGACTCGAATGTAAG+CGG | 0.610274 | 7.3:-87073370 | MS.gene79568:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTGAATTTGTAAAGATTA+TGG | - | chr7.3:87073581-87073600 | MS.gene79568:CDS | 20.0% |
| !!! | AAAATGATCAAGAATTTTGA+TGG | - | chr7.3:87073534-87073553 | MS.gene79568:CDS | 20.0% |
| ! | GATGAACTTGAAGAAAAATA+TGG | - | chr7.3:87073339-87073358 | MS.gene79568:CDS | 25.0% |
| ! | TGAAGAAAAATATGGTTCAA+AGG | - | chr7.3:87073347-87073366 | MS.gene79568:CDS | 25.0% |
| ! | TTGTAAAGATTATGGAAAAC+AGG | - | chr7.3:87073589-87073608 | MS.gene79568:CDS | 25.0% |
| !! | CAAAGAGTTATGTGTATTTT+GGG | - | chr7.3:87073477-87073496 | MS.gene79568:CDS | 25.0% |
| !!! | AATTTTGATGGAAATCAAGA+TGG | - | chr7.3:87073546-87073565 | MS.gene79568:CDS | 25.0% |
| !!! | TATGTGTATTTTGGGATTTA+AGG | - | chr7.3:87073485-87073504 | MS.gene79568:CDS | 25.0% |
| ACTTGTTTATCTTATTGCAG+AGG | - | chr7.3:87073026-87073045 | MS.gene79568:CDS | 30.0% | |
| CTGCAATAAGATAAACAAGT+TGG | + | chr7.3:87073026-87073045 | None:intergenic | 30.0% | |
| ! | GCAAAGAGTTATGTGTATTT+TGG | - | chr7.3:87073476-87073495 | MS.gene79568:CDS | 30.0% |
| ! | GTTTTTGATGAGAACAAAGA+TGG | - | chr7.3:87073432-87073451 | MS.gene79568:CDS | 30.0% |
| !!! | TGTATTTTGGGATTTAAGGA+AGG | - | chr7.3:87073489-87073508 | MS.gene79568:CDS | 30.0% |
| AGAAAGTGGAGAGATTAAGA+GGG | - | chr7.3:87073263-87073282 | MS.gene79568:CDS | 35.0% | |
| GAAAGCAACTCAACTTTGTT+TGG | - | chr7.3:87073069-87073088 | MS.gene79568:CDS | 35.0% | |
| ! | ACAAGTTCTTCTCGAGTTTT+TGG | - | chr7.3:87073136-87073155 | MS.gene79568:CDS | 35.0% |
| AAGTTTGGAGGAAGTGAAGA+TGG | - | chr7.3:87073401-87073420 | MS.gene79568:CDS | 40.0% | |
| ATCTTCACTTCCTCCAAACT+TGG | + | chr7.3:87073402-87073421 | None:intergenic | 40.0% | |
| GAGAAAGTGGAGAGATTAAG+AGG | - | chr7.3:87073262-87073281 | MS.gene79568:CDS | 40.0% | |
| TAAGAGGGATGAAGTTGAAG+CGG | - | chr7.3:87073278-87073297 | MS.gene79568:CDS | 40.0% | |
| TCCCTTTACTTCTAAGTCAC+CGG | + | chr7.3:87073187-87073206 | None:intergenic | 40.0% | |
| TCCGGTGACTTAGAAGTAAA+GGG | - | chr7.3:87073183-87073202 | MS.gene79568:CDS | 40.0% | |
| TGATGAGAATGAGCCAAGTT+TGG | - | chr7.3:87073386-87073405 | MS.gene79568:CDS | 40.0% | |
| TTCCGGTGACTTAGAAGTAA+AGG | - | chr7.3:87073182-87073201 | MS.gene79568:CDS | 40.0% | |
| AGCAGAGACTCGAATGTAAG+CGG | - | chr7.3:87073240-87073259 | MS.gene79568:CDS | 45.0% | |
| CTAAGTCACCGGAACGAAAT+TGG | + | chr7.3:87073176-87073195 | None:intergenic | 45.0% | |
| CTTCTCTGCCAATTTCGTTC+CGG | - | chr7.3:87073165-87073184 | MS.gene79568:CDS | 45.0% | |
| TCGAATGTAAGCGGAGAAAG+TGG | - | chr7.3:87073249-87073268 | MS.gene79568:CDS | 45.0% | |
| TGAGAATGAGCCAAGTTTGG+AGG | - | chr7.3:87073389-87073408 | MS.gene79568:CDS | 45.0% | |
| CGGAACGAAATTGGCAGAGA+AGG | + | chr7.3:87073167-87073186 | None:intergenic | 50.0% | |
| GGATGAAGTTGAAGCGGTGA+TGG | - | chr7.3:87073284-87073303 | MS.gene79568:CDS | 50.0% | |
| ! | GAAGCGGTGATGGCGAAAAT+GGG | - | chr7.3:87073294-87073313 | MS.gene79568:CDS | 50.0% |
| ! | TGAAGCGGTGATGGCGAAAA+TGG | - | chr7.3:87073293-87073312 | MS.gene79568:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 87073012 | 87073620 | 87073012 | ID=MS.gene79568 |
| chr7.3 | mRNA | 87073012 | 87073620 | 87073012 | ID=MS.gene79568.t1;Parent=MS.gene79568 |
| chr7.3 | exon | 87073012 | 87073620 | 87073012 | ID=MS.gene79568.t1.exon1;Parent=MS.gene79568.t1 |
| chr7.3 | CDS | 87073012 | 87073620 | 87073012 | ID=cds.MS.gene79568.t1;Parent=MS.gene79568.t1 |
| Gene Sequence |
| Protein sequence |
