Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48885.t1 | XP_003605209.1 | 93.5 | 186 | 11 | 1 | 1 | 186 | 1 | 185 | 7.60E-91 | 343.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48885.t1 | Q9SHC8 | 50.5 | 198 | 67 | 3 | 19 | 186 | 6 | 202 | 9.2e-45 | 181.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48885.t1 | G7JH03 | 93.5 | 186 | 11 | 1 | 1 | 186 | 1 | 185 | 5.5e-91 | 343.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene48885 | -0.805229 | 1.48E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene48885 | -0.802593 | 5.21E-49 | -1.69E-46 |
| MS.gene04925 | MS.gene48885 | 0.818161 | 2.27E-52 | -1.69E-46 |
| MS.gene04926 | MS.gene48885 | 0.837568 | 4.81E-57 | -1.69E-46 |
| MS.gene049549 | MS.gene48885 | 0.830377 | 3.04E-55 | -1.69E-46 |
| MS.gene049805 | MS.gene48885 | -0.800843 | 1.19E-48 | -1.69E-46 |
| MS.gene049813 | MS.gene48885 | 0.809145 | 2.19E-50 | -1.69E-46 |
| MS.gene050095 | MS.gene48885 | 0.818266 | 2.14E-52 | -1.69E-46 |
| MS.gene050096 | MS.gene48885 | 0.81725 | 3.64E-52 | -1.69E-46 |
| MS.gene050315 | MS.gene48885 | -0.80282 | 4.68E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene48885 | 0.824419 | 8.15E-54 | -1.69E-46 |
| MS.gene050479 | MS.gene48885 | 0.822757 | 2.00E-53 | -1.69E-46 |
| MS.gene050547 | MS.gene48885 | 0.812769 | 3.59E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene48885 | -0.802204 | 6.27E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene48885 | 0.8121 | 5.02E-51 | -1.69E-46 |
| MS.gene050720 | MS.gene48885 | 0.815927 | 7.19E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene48885 | -0.818317 | 2.09E-52 | -1.69E-46 |
| MS.gene050822 | MS.gene48885 | 0.806538 | 7.84E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene48885 | -0.802914 | 4.47E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene48885 | -0.803281 | 3.76E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene48885 | -0.813431 | 2.57E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene48885 | 0.813032 | 3.14E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene48885 | 0.808989 | 2.36E-50 | -1.69E-46 |
| MS.gene051385 | MS.gene48885 | 0.826333 | 2.87E-54 | -1.69E-46 |
| MS.gene051440 | MS.gene48885 | 0.810876 | 9.27E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene48885 | -0.801436 | 9.01E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene48885 | 0.822253 | 2.62E-53 | -1.69E-46 |
| MS.gene051996 | MS.gene48885 | 0.846441 | 2.17E-59 | -1.69E-46 |
| MS.gene052164 | MS.gene48885 | -0.8146 | 1.42E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene48885 | 0.852662 | 3.98E-61 | -1.69E-46 |
| MS.gene052212 | MS.gene48885 | 0.817481 | 3.22E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene48885 | 0.884106 | 2.54E-71 | -1.69E-46 |
| MS.gene052501 | MS.gene48885 | -0.816578 | 5.15E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene48885 | -0.81556 | 8.69E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene48885 | -0.813417 | 2.59E-51 | -1.69E-46 |
| MS.gene052606 | MS.gene48885 | 0.826768 | 2.26E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene48885 | -0.803965 | 2.71E-49 | -1.69E-46 |
| MS.gene052705 | MS.gene48885 | 0.803851 | 2.86E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene48885 | -0.818714 | 1.70E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene48885 | -0.819902 | 9.10E-53 | -1.69E-46 |
| MS.gene052982 | MS.gene48885 | -0.802058 | 6.72E-49 | -1.69E-46 |
| MS.gene053097 | MS.gene48885 | 0.804029 | 2.63E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene48885 | -0.829205 | 5.87E-55 | -1.69E-46 |
| MS.gene053319 | MS.gene48885 | 0.807036 | 6.15E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene48885 | 0.831785 | 1.37E-55 | -1.69E-46 |
| MS.gene053406 | MS.gene48885 | 0.868374 | 6.85E-66 | -1.69E-46 |
| MS.gene053436 | MS.gene48885 | -0.81039 | 1.18E-50 | -1.69E-46 |
| MS.gene053579 | MS.gene48885 | -0.807638 | 4.58E-50 | -1.69E-46 |
| MS.gene053887 | MS.gene48885 | 0.802618 | 5.15E-49 | -1.69E-46 |
| MS.gene054146 | MS.gene48885 | 0.831231 | 1.88E-55 | -1.69E-46 |
| MS.gene054147 | MS.gene48885 | 0.849529 | 3.05E-60 | -1.69E-46 |
| MS.gene054277 | MS.gene48885 | 0.862778 | 3.98E-64 | -1.69E-46 |
| MS.gene054421 | MS.gene48885 | 0.808758 | 2.65E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene48885 | 0.809461 | 1.87E-50 | -1.69E-46 |
| MS.gene054707 | MS.gene48885 | 0.806097 | 9.70E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene48885 | -0.823649 | 1.24E-53 | -1.69E-46 |
| MS.gene054742 | MS.gene48885 | 0.890803 | 7.08E-74 | -1.69E-46 |
| MS.gene054884 | MS.gene48885 | 0.818369 | 2.03E-52 | -1.69E-46 |
| MS.gene054927 | MS.gene48885 | -0.826551 | 2.55E-54 | -1.69E-46 |
| MS.gene055124 | MS.gene48885 | 0.808916 | 2.45E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene48885 | 0.83029 | 3.19E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene48885 | -0.800592 | 1.34E-48 | -1.69E-46 |
| MS.gene055370 | MS.gene48885 | -0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene055502 | MS.gene48885 | -0.80595 | 1.04E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene48885 | -0.805461 | 1.32E-49 | -1.69E-46 |
| MS.gene055601 | MS.gene48885 | 0.80982 | 1.57E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene48885 | -0.805797 | 1.12E-49 | -1.69E-46 |
| MS.gene056040 | MS.gene48885 | -0.808289 | 3.33E-50 | -1.69E-46 |
| MS.gene056076 | MS.gene48885 | 0.837226 | 5.89E-57 | -1.69E-46 |
| MS.gene056218 | MS.gene48885 | 0.839904 | 1.20E-57 | -1.69E-46 |
| MS.gene056313 | MS.gene48885 | 0.804124 | 2.51E-49 | -1.69E-46 |
| MS.gene056330 | MS.gene48885 | 0.83162 | 1.51E-55 | -1.69E-46 |
| MS.gene056590 | MS.gene48885 | 0.835659 | 1.47E-56 | -1.69E-46 |
| MS.gene056591 | MS.gene48885 | 0.83649 | 9.07E-57 | -1.69E-46 |
| MS.gene056680 | MS.gene48885 | 0.853874 | 1.79E-61 | -1.69E-46 |
| MS.gene056748 | MS.gene48885 | 0.848119 | 7.51E-60 | -1.69E-46 |
| MS.gene056920 | MS.gene48885 | 0.856689 | 2.72E-62 | -1.69E-46 |
| MS.gene056922 | MS.gene48885 | 0.807557 | 4.77E-50 | -1.69E-46 |
| MS.gene056988 | MS.gene48885 | 0.815762 | 7.83E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene48885 | -0.816637 | 4.99E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene48885 | 0.831142 | 1.97E-55 | -1.69E-46 |
| MS.gene057291 | MS.gene48885 | 0.802792 | 4.74E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene48885 | 0.823329 | 1.47E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene48885 | -0.800193 | 1.62E-48 | -1.69E-46 |
| MS.gene057770 | MS.gene48885 | 0.805146 | 1.54E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene48885 | 0.80209 | 6.62E-49 | -1.69E-46 |
| MS.gene058117 | MS.gene48885 | 0.800897 | 1.16E-48 | -1.69E-46 |
| MS.gene058177 | MS.gene48885 | 0.804584 | 2.01E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene48885 | -0.822326 | 2.51E-53 | -1.69E-46 |
| MS.gene058381 | MS.gene48885 | 0.823399 | 1.41E-53 | -1.69E-46 |
| MS.gene058418 | MS.gene48885 | 0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene48885 | -0.81763 | 2.98E-52 | -1.69E-46 |
| MS.gene058603 | MS.gene48885 | 0.811755 | 5.97E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene48885 | -0.816347 | 5.79E-52 | -1.69E-46 |
| MS.gene05883 | MS.gene48885 | -0.808559 | 2.92E-50 | -1.69E-46 |
| MS.gene059060 | MS.gene48885 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene05914 | MS.gene48885 | -0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene059303 | MS.gene48885 | -0.809666 | 1.69E-50 | -1.69E-46 |
| MS.gene059328 | MS.gene48885 | 0.805948 | 1.04E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene48885 | -0.802421 | 5.66E-49 | -1.69E-46 |
| MS.gene059891 | MS.gene48885 | 0.838139 | 3.43E-57 | -1.69E-46 |
| MS.gene059892 | MS.gene48885 | 0.838491 | 2.79E-57 | -1.69E-46 |
| MS.gene059953 | MS.gene48885 | 0.830388 | 3.02E-55 | -1.69E-46 |
| MS.gene059955 | MS.gene48885 | 0.827363 | 1.63E-54 | -1.69E-46 |
| MS.gene060087 | MS.gene48885 | 0.874444 | 6.72E-68 | -1.69E-46 |
| MS.gene060358 | MS.gene48885 | 0.83281 | 7.64E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene48885 | -0.800686 | 1.28E-48 | -1.69E-46 |
| MS.gene06092 | MS.gene48885 | -0.800581 | 1.35E-48 | -1.69E-46 |
| MS.gene061038 | MS.gene48885 | 0.833629 | 4.78E-56 | -1.69E-46 |
| MS.gene061092 | MS.gene48885 | 0.812457 | 4.20E-51 | -1.69E-46 |
| MS.gene061094 | MS.gene48885 | 0.812763 | 3.60E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48885.t1 | MTR_4g026620 | 93.548 | 186 | 11 | 1 | 1 | 186 | 1 | 185 | 1.40e-125 | 352 |
| MS.gene48885.t1 | MTR_4g026830 | 67.708 | 192 | 52 | 4 | 1 | 186 | 1 | 188 | 2.45e-82 | 243 |
| MS.gene48885.t1 | MTR_4g027050 | 58.794 | 199 | 50 | 3 | 19 | 186 | 7 | 204 | 2.55e-74 | 223 |
| MS.gene48885.t1 | MTR_4g026780 | 63.351 | 191 | 62 | 2 | 1 | 186 | 1 | 188 | 1.34e-68 | 207 |
| MS.gene48885.t1 | MTR_4g026650 | 70.423 | 142 | 36 | 1 | 1 | 136 | 1 | 142 | 9.94e-68 | 204 |
| MS.gene48885.t1 | MTR_4g026780 | 62.766 | 188 | 52 | 3 | 1 | 183 | 1 | 175 | 6.03e-67 | 202 |
| MS.gene48885.t1 | MTR_4g026780 | 60.302 | 199 | 56 | 3 | 1 | 183 | 1 | 192 | 2.06e-66 | 202 |
| MS.gene48885.t1 | MTR_4g026780 | 60.000 | 205 | 63 | 3 | 1 | 186 | 1 | 205 | 2.93e-65 | 200 |
| MS.gene48885.t1 | MTR_0135s0080 | 69.427 | 157 | 43 | 1 | 1 | 152 | 1 | 157 | 3.14e-65 | 197 |
| MS.gene48885.t1 | MTR_4g026780 | 60.000 | 205 | 63 | 3 | 1 | 186 | 1 | 205 | 3.36e-65 | 201 |
| MS.gene48885.t1 | MTR_4g101020 | 48.768 | 203 | 73 | 3 | 15 | 186 | 4 | 206 | 5.85e-59 | 184 |
| MS.gene48885.t1 | MTR_5g012420 | 50.251 | 199 | 68 | 3 | 19 | 186 | 9 | 207 | 2.41e-57 | 180 |
| MS.gene48885.t1 | MTR_4g118760 | 58.647 | 133 | 55 | 0 | 19 | 151 | 6 | 138 | 2.26e-47 | 156 |
| MS.gene48885.t1 | MTR_4g118760 | 58.647 | 133 | 55 | 0 | 19 | 151 | 6 | 138 | 2.88e-47 | 156 |
| MS.gene48885.t1 | MTR_5g013320 | 43.429 | 175 | 87 | 2 | 19 | 186 | 7 | 176 | 2.34e-41 | 139 |
| MS.gene48885.t1 | MTR_4g026540 | 46.111 | 180 | 83 | 3 | 21 | 186 | 9 | 188 | 9.29e-41 | 137 |
| MS.gene48885.t1 | MTR_1g081950 | 47.368 | 133 | 70 | 0 | 19 | 151 | 7 | 139 | 1.02e-35 | 129 |
| MS.gene48885.t1 | MTR_4g026730 | 68.966 | 58 | 8 | 1 | 80 | 137 | 78 | 125 | 7.54e-22 | 86.3 |
| MS.gene48885.t1 | MTR_0753s0030 | 58.228 | 79 | 30 | 2 | 31 | 106 | 1 | 79 | 9.04e-22 | 84.7 |
| MS.gene48885.t1 | MTR_5g012420 | 40.000 | 135 | 50 | 3 | 83 | 186 | 1 | 135 | 7.00e-21 | 85.1 |
| MS.gene48885.t1 | MTR_4g026600 | 64.286 | 56 | 19 | 1 | 79 | 134 | 23 | 77 | 7.13e-15 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48885.t1 | AT2G45140 | 54.696 | 181 | 68 | 3 | 19 | 186 | 6 | 185 | 3.16e-62 | 192 |
| MS.gene48885.t1 | AT2G45140 | 52.284 | 197 | 65 | 5 | 19 | 186 | 6 | 202 | 6.46e-61 | 189 |
| MS.gene48885.t1 | AT3G60600 | 48.148 | 216 | 78 | 6 | 5 | 186 | 4 | 219 | 8.48e-61 | 190 |
| MS.gene48885.t1 | AT3G60600 | 54.545 | 154 | 64 | 2 | 5 | 153 | 4 | 156 | 1.02e-54 | 173 |
| MS.gene48885.t1 | AT3G60600 | 57.639 | 144 | 60 | 1 | 10 | 153 | 14 | 156 | 1.18e-54 | 173 |
| MS.gene48885.t1 | AT4G00170 | 45.960 | 198 | 75 | 4 | 19 | 186 | 7 | 202 | 3.10e-49 | 160 |
| MS.gene48885.t1 | AT2G23830 | 52.555 | 137 | 64 | 1 | 19 | 154 | 7 | 143 | 7.47e-46 | 148 |
| MS.gene48885.t1 | AT5G47180 | 42.857 | 168 | 96 | 0 | 19 | 186 | 10 | 177 | 2.16e-43 | 144 |
| MS.gene48885.t1 | AT5G47180 | 42.857 | 168 | 96 | 0 | 19 | 186 | 10 | 177 | 2.16e-43 | 144 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 160 | 310 | 6.79e-40 | 142 |
| MS.gene48885.t1 | AT1G51270 | 48.413 | 126 | 63 | 1 | 25 | 150 | 13 | 136 | 1.76e-36 | 133 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 160 | 310 | 6.79e-40 | 142 |
| MS.gene48885.t1 | AT1G51270 | 48.413 | 126 | 63 | 1 | 25 | 150 | 13 | 136 | 1.76e-36 | 133 |
| MS.gene48885.t1 | AT1G08820 | 49.007 | 151 | 71 | 2 | 19 | 165 | 6 | 154 | 8.75e-40 | 139 |
| MS.gene48885.t1 | AT1G08820 | 49.007 | 151 | 71 | 2 | 19 | 165 | 6 | 154 | 8.75e-40 | 139 |
| MS.gene48885.t1 | AT1G08820 | 49.007 | 151 | 71 | 2 | 19 | 165 | 6 | 154 | 8.75e-40 | 139 |
| MS.gene48885.t1 | AT1G08820 | 49.007 | 151 | 71 | 2 | 19 | 165 | 6 | 154 | 8.75e-40 | 139 |
| MS.gene48885.t1 | AT1G08820 | 49.007 | 151 | 71 | 2 | 19 | 165 | 6 | 154 | 8.75e-40 | 139 |
| MS.gene48885.t1 | AT1G51270 | 48.889 | 135 | 68 | 1 | 19 | 153 | 6 | 139 | 1.40e-39 | 138 |
| MS.gene48885.t1 | AT1G51270 | 48.889 | 135 | 68 | 1 | 19 | 153 | 6 | 139 | 1.40e-39 | 138 |
| MS.gene48885.t1 | AT1G51270 | 48.889 | 135 | 68 | 1 | 19 | 153 | 6 | 139 | 1.40e-39 | 138 |
| MS.gene48885.t1 | AT1G51270 | 48.889 | 135 | 68 | 1 | 19 | 153 | 6 | 139 | 1.40e-39 | 138 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 160 | 310 | 1.65e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 48.413 | 126 | 63 | 1 | 25 | 150 | 13 | 136 | 4.81e-36 | 132 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 112 | 262 | 2.19e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 47.191 | 89 | 45 | 1 | 62 | 150 | 2 | 88 | 4.82e-22 | 92.8 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 112 | 262 | 2.19e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 47.191 | 89 | 45 | 1 | 62 | 150 | 2 | 88 | 4.82e-22 | 92.8 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 112 | 262 | 2.19e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 47.191 | 89 | 45 | 1 | 62 | 150 | 2 | 88 | 4.82e-22 | 92.8 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 112 | 262 | 2.19e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 47.191 | 89 | 45 | 1 | 62 | 150 | 2 | 88 | 4.82e-22 | 92.8 |
| MS.gene48885.t1 | AT1G51270 | 46.405 | 153 | 80 | 2 | 1 | 153 | 160 | 310 | 2.61e-39 | 141 |
| MS.gene48885.t1 | AT1G51270 | 48.413 | 126 | 63 | 1 | 25 | 150 | 13 | 136 | 7.39e-36 | 132 |
| MS.gene48885.t1 | AT1G51270 | 50.388 | 129 | 63 | 1 | 25 | 153 | 13 | 140 | 1.19e-38 | 135 |
| MS.gene48885.t1 | AT1G51270 | 48.889 | 135 | 68 | 1 | 19 | 153 | 6 | 139 | 2.60e-38 | 137 |
| MS.gene48885.t1 | AT4G00170 | 42.938 | 177 | 69 | 4 | 40 | 186 | 1 | 175 | 5.99e-38 | 130 |
| MS.gene48885.t1 | AT1G51270 | 43.662 | 71 | 39 | 1 | 83 | 153 | 1 | 70 | 4.12e-12 | 63.9 |
Find 30 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAATTTAACAACAGAAAT+TGG | 0.206639 | 4.3:+72143333 | None:intergenic |
| CCATAGTTAACTTTGAAATT+TGG | 0.216610 | 4.3:+72141617 | None:intergenic |
| AGGTCTACTTGTGATATTAC+AGG | 0.287192 | 4.3:-72142893 | MS.gene48885:intron |
| CCAAATTTCAAAGTTAACTA+TGG | 0.289882 | 4.3:-72141617 | MS.gene48885:CDS |
| ATTTGATTTATTAGATAATC+AGG | 0.314959 | 4.3:-72141866 | MS.gene48885:intron |
| GCTGGTGATGATGGTGGGTT+AGG | 0.351878 | 4.3:+72141901 | None:intergenic |
| TAACAAGTCTGATAATTATC+TGG | 0.360749 | 4.3:-72143073 | MS.gene48885:CDS |
| TTATCGATGCAATGCATGTT+AGG | 0.402557 | 4.3:+72142130 | None:intergenic |
| TATCAGACTTGTTAGACATC+TGG | 0.410398 | 4.3:+72143082 | None:intergenic |
| TTCCTGAGAAATATTGTGTT+AGG | 0.414467 | 4.3:-72142940 | MS.gene48885:CDS |
| TATTGTGTTAGGCCTAATAA+TGG | 0.425729 | 4.3:-72142929 | MS.gene48885:CDS |
| TGATAATTATCTGGCTTTCA+AGG | 0.427384 | 4.3:-72143064 | MS.gene48885:intron |
| GCACATTTCAACCTTATATC+CGG | 0.430207 | 4.3:+72141954 | None:intergenic |
| GATGCTTGAGCTGGTGATGA+TGG | 0.449532 | 4.3:+72141892 | None:intergenic |
| GGCAACACAACTCCATTATT+AGG | 0.451335 | 4.3:+72142917 | None:intergenic |
| ATATCTTCTGGGGTTGCACC+AGG | 0.458454 | 4.3:+72142079 | None:intergenic |
| GGCCTAACACAATATTTCTC+AGG | 0.461582 | 4.3:+72142938 | None:intergenic |
| CATGGAAAGTGTAGCTCTTG+AGG | 0.476067 | 4.3:+72143276 | None:intergenic |
| ATGGAAAGTGTAGCTCTTGA+GGG | 0.486542 | 4.3:+72143277 | None:intergenic |
| GGTGATGATGGTGGGTTAGG+AGG | 0.488542 | 4.3:+72141904 | None:intergenic |
| TACAATGCAAGCTCAAAAGA+AGG | 0.541868 | 4.3:-72142158 | MS.gene48885:CDS |
| GATGATGGTGGGTTAGGAGG+AGG | 0.542814 | 4.3:+72141907 | None:intergenic |
| CAAAGCATAGTCGCAAAGCC+TGG | 0.544647 | 4.3:-72142097 | MS.gene48885:CDS |
| ATAATGGAGTTGTGTTGCCT+AGG | 0.546547 | 4.3:-72142913 | MS.gene48885:CDS |
| GCTTGAGCTGGTGATGATGG+TGG | 0.553871 | 4.3:+72141895 | None:intergenic |
| TCAAATTCTGATGCTTGAGC+TGG | 0.556015 | 4.3:+72141883 | None:intergenic |
| CTTGAGCTGGTGATGATGGT+GGG | 0.578737 | 4.3:+72141896 | None:intergenic |
| GCTCTTGAGGGTGTATCTGA+AGG | 0.583919 | 4.3:+72143289 | None:intergenic |
| GTAATATCACAAGTAGACCT+AGG | 0.632852 | 4.3:+72142896 | None:intergenic |
| TCGATGCAATGCATGTTAGG+TGG | 0.713518 | 4.3:+72142133 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAATTAATTTAATATT+TGG | + | chr4.3:72143278-72143297 | None:intergenic | 0.0% |
| !!! | CTTGAATTTTTTATTATTTT+AGG | - | chr4.3:72143005-72143024 | MS.gene48885:intron | 10.0% |
| !!! | TATATATTTTTTTGGTTTTT+GGG | + | chr4.3:72141421-72141440 | None:intergenic | 10.0% |
| !!! | TTATAATTTTGATTTAGAAA+AGG | - | chr4.3:72141663-72141682 | MS.gene48885:intron | 10.0% |
| !!! | TTATATATTTTTTTGGTTTT+TGG | + | chr4.3:72141422-72141441 | None:intergenic | 10.0% |
| !!! | TTCTCATTTATATATTTTTT+TGG | + | chr4.3:72141429-72141448 | None:intergenic | 10.0% |
| !!! | TTTTATATATTGTGTTATTT+AGG | - | chr4.3:72141635-72141654 | MS.gene48885:intron | 10.0% |
| !! | ATCTTTATAAAAACAAAACA+TGG | - | chr4.3:72141939-72141958 | MS.gene48885:CDS | 15.0% |
| !! | TCTTTATAAAAACAAAACAT+GGG | - | chr4.3:72141940-72141959 | MS.gene48885:CDS | 15.0% |
| !!! | ATTTGATTTATTAGATAATC+AGG | - | chr4.3:72142786-72142805 | MS.gene48885:intron | 15.0% |
| !!! | TATTTTCTTTAACACTTTTT+TGG | - | chr4.3:72141501-72141520 | MS.gene48885:intron | 15.0% |
| !!! | TTTTTTTTTTTTTGTCAAGT+AGG | - | chr4.3:72142022-72142041 | MS.gene48885:intron | 15.0% |
| !! | GCACAATATACAATATTAAT+TGG | - | chr4.3:72142845-72142864 | MS.gene48885:intron | 20.0% |
| !! | TATGAGTTATCAATTGTAAA+AGG | - | chr4.3:72142235-72142254 | MS.gene48885:intron | 20.0% |
| !! | TTAATATTTGGAAACCAAAT+AGG | + | chr4.3:72143266-72143285 | None:intergenic | 20.0% |
| !! | TTCAATTTAACAACAGAAAT+TGG | + | chr4.3:72141322-72141341 | None:intergenic | 20.0% |
| !!! | CACATTTTATCTTAAAGTTT+TGG | - | chr4.3:72143148-72143167 | MS.gene48885:intron | 20.0% |
| !!! | GTTTTTATAAAGATAAGCTA+AGG | + | chr4.3:72141933-72141952 | None:intergenic | 20.0% |
| !!! | TATATTGTGTTATTTAGGAA+AGG | - | chr4.3:72141640-72141659 | MS.gene48885:intron | 20.0% |
| !!! | TCAACTATTTGTTTTTTTAG+AGG | + | chr4.3:72142311-72142330 | None:intergenic | 20.0% |
| ! | AACAAGACACTAAATGAAAA+AGG | + | chr4.3:72141469-72141488 | None:intergenic | 25.0% |
| ! | AAGATTTACCATGAAAACAT+TGG | + | chr4.3:72142395-72142414 | None:intergenic | 25.0% |
| ! | AGAAGATATCACTTCAAAAA+TGG | - | chr4.3:72142587-72142606 | MS.gene48885:intron | 25.0% |
| ! | ATTCACCAACATAAATCAAT+AGG | + | chr4.3:72142879-72142898 | None:intergenic | 25.0% |
| ! | CCAAATTTCAAAGTTAACTA+TGG | - | chr4.3:72143035-72143054 | MS.gene48885:intron | 25.0% |
| ! | TAACAAGTCTGATAATTATC+TGG | - | chr4.3:72141579-72141598 | MS.gene48885:CDS | 25.0% |
| ! | TACTATTTACATGAACAACT+TGG | - | chr4.3:72143184-72143203 | MS.gene48885:intron | 25.0% |
| ! | TGAAAGTGAGAATAAAAGAA+CGG | + | chr4.3:72142435-72142454 | None:intergenic | 25.0% |
| ! | TTCACCAACATAAATCAATA+GGG | + | chr4.3:72142878-72142897 | None:intergenic | 25.0% |
| !! | AAAACAAATAGTTGATGTGT+TGG | - | chr4.3:72142316-72142335 | MS.gene48885:intron | 25.0% |
| !! | TTTATTCTCCAATGTTTTCA+TGG | - | chr4.3:72142384-72142403 | MS.gene48885:intron | 25.0% |
| !!! | ATGTAGTTTTTTCAAGATTC+CGG | - | chr4.3:72142679-72142698 | MS.gene48885:intron | 25.0% |
| !!! | ATTTTTGAAGTGATATCTTC+TGG | + | chr4.3:72142588-72142607 | None:intergenic | 25.0% |
| !!! | CCATAGTTAACTTTGAAATT+TGG | + | chr4.3:72143038-72143057 | None:intergenic | 25.0% |
| !!! | TTTTTGAAGTGATATCTTCT+GGG | + | chr4.3:72142587-72142606 | None:intergenic | 25.0% |
| AAATCACATTGAGACCTATT+TGG | - | chr4.3:72143249-72143268 | MS.gene48885:intron | 30.0% | |
| ATCAATTGAGCTACATTTAC+TGG | - | chr4.3:72142134-72142153 | MS.gene48885:CDS | 30.0% | |
| TATTGTGTTAGGCCTAATAA+TGG | - | chr4.3:72141723-72141742 | MS.gene48885:intron | 30.0% | |
| TTCCTGAGAAATATTGTGTT+AGG | - | chr4.3:72141712-72141731 | MS.gene48885:intron | 30.0% | |
| ! | TAGTTTATGTTTGCTGAAAG+TGG | - | chr4.3:72142169-72142188 | MS.gene48885:intron | 30.0% |
| ! | TTATGTTGGTGAATAAGAAG+TGG | - | chr4.3:72142885-72142904 | MS.gene48885:intron | 30.0% |
| !! | TGATAATTATCTGGCTTTCA+AGG | - | chr4.3:72141588-72141607 | MS.gene48885:CDS | 30.0% |
| !! | TTTTCAAGATTCCGGATATA+AGG | - | chr4.3:72142687-72142706 | MS.gene48885:intron | 30.0% |
| !!! | TTTTGAAGTGATATCTTCTG+GGG | + | chr4.3:72142586-72142605 | None:intergenic | 30.0% |
| AGATACACACACATTATGTG+TGG | - | chr4.3:72142054-72142073 | MS.gene48885:intron | 35.0% | |
| AGGTCTACTTGTGATATTAC+AGG | - | chr4.3:72141759-72141778 | MS.gene48885:intron | 35.0% | |
| ATTGAAGAGGCTTAGTTACA+TGG | + | chr4.3:72141397-72141416 | None:intergenic | 35.0% | |
| GCACATTTCAACCTTATATC+CGG | + | chr4.3:72142701-72142720 | None:intergenic | 35.0% | |
| GTAATATCACAAGTAGACCT+AGG | + | chr4.3:72141759-72141778 | None:intergenic | 35.0% | |
| TAAAAAGCTTCAGCAAGAAC+TGG | - | chr4.3:72143083-72143102 | MS.gene48885:CDS | 35.0% | |
| TACAATGCAAGCTCAAAAGA+AGG | - | chr4.3:72142494-72142513 | MS.gene48885:intron | 35.0% | |
| TTATCGATGCAATGCATGTT+AGG | + | chr4.3:72142525-72142544 | None:intergenic | 35.0% | |
| ! | GAACAACTTTTGAATGCTCA+AGG | + | chr4.3:72141983-72142002 | None:intergenic | 35.0% |
| ! | GCGTTCAGATAAGTTGAATA+AGG | + | chr4.3:72143323-72143342 | None:intergenic | 35.0% |
| !! | CTGACCCTATTGATTTATGT+TGG | - | chr4.3:72142871-72142890 | MS.gene48885:intron | 35.0% |
| !! | TATCAGACTTGTTAGACATC+TGG | + | chr4.3:72141573-72141592 | None:intergenic | 35.0% |
| !! | TTGGTTTTTGGGAATTGAAG+AGG | + | chr4.3:72141410-72141429 | None:intergenic | 35.0% |
| !! | TTTTGAATGCTCAAGGTATC+AGG | + | chr4.3:72141976-72141995 | None:intergenic | 35.0% |
| !!! | TTTTTTGTCAAGTAGGCTAG+TGG | - | chr4.3:72142029-72142048 | MS.gene48885:intron | 35.0% |
| ACATTATGTGTGGAGAAGTG+TGG | - | chr4.3:72142064-72142083 | MS.gene48885:intron | 40.0% | |
| GGCAACACAACTCCATTATT+AGG | + | chr4.3:72141738-72141757 | None:intergenic | 40.0% | |
| GGCCTAACACAATATTTCTC+AGG | + | chr4.3:72141717-72141736 | None:intergenic | 40.0% | |
| TAGAAAAGGTTGCAACTTGC+AGG | - | chr4.3:72141677-72141696 | MS.gene48885:intron | 40.0% | |
| TCAAATTCTGATGCTTGAGC+TGG | + | chr4.3:72142772-72142791 | None:intergenic | 40.0% | |
| ! | AGCTCAATTGATAGCTACCA+GGG | + | chr4.3:72142126-72142145 | None:intergenic | 40.0% |
| ! | ATAATGGAGTTGTGTTGCCT+AGG | - | chr4.3:72141739-72141758 | MS.gene48885:intron | 40.0% |
| ! | ATGGAAAGTGTAGCTCTTGA+GGG | + | chr4.3:72141378-72141397 | None:intergenic | 40.0% |
| ! | TACCAGGGATAATTTGTGCA+GGG | + | chr4.3:72142111-72142130 | None:intergenic | 40.0% |
| ! | TAGCTCAATTGATAGCTACC+AGG | + | chr4.3:72142127-72142146 | None:intergenic | 40.0% |
| TCGATGCAATGCATGTTAGG+TGG | + | chr4.3:72142522-72142541 | None:intergenic | 45.0% | |
| TCGTGCTCTTGTATGCAAGA+GGG | + | chr4.3:72141810-72141829 | None:intergenic | 45.0% | |
| TTCGTGCTCTTGTATGCAAG+AGG | + | chr4.3:72141811-72141830 | None:intergenic | 45.0% | |
| ! | ACCAGGGATAATTTGTGCAG+GGG | + | chr4.3:72142110-72142129 | None:intergenic | 45.0% |
| ! | CATGGAAAGTGTAGCTCTTG+AGG | + | chr4.3:72141379-72141398 | None:intergenic | 45.0% |
| ! | CTACCAGGGATAATTTGTGC+AGG | + | chr4.3:72142112-72142131 | None:intergenic | 45.0% |
| CAAAGCATAGTCGCAAAGCC+TGG | - | chr4.3:72142555-72142574 | MS.gene48885:intron | 50.0% | |
| CTTGTATGCAAGAGGGAGAG+TGG | + | chr4.3:72141803-72141822 | None:intergenic | 50.0% | |
| ! | GATAATTTGTGCAGGGGCCA+GGG | + | chr4.3:72142104-72142123 | None:intergenic | 50.0% |
| ! | GATGCTTGAGCTGGTGATGA+TGG | + | chr4.3:72142763-72142782 | None:intergenic | 50.0% |
| ! | GCTCTTGAGGGTGTATCTGA+AGG | + | chr4.3:72141366-72141385 | None:intergenic | 50.0% |
| ! | GTGTGGAGAAGTGTGGAATC+CGG | - | chr4.3:72142071-72142090 | MS.gene48885:CDS | 50.0% |
| ! | TGTGGAGAAGTGTGGAATCC+GGG | - | chr4.3:72142072-72142091 | MS.gene48885:CDS | 50.0% |
| !! | ATATCTTCTGGGGTTGCACC+AGG | + | chr4.3:72142576-72142595 | None:intergenic | 50.0% |
| !! | CTTGAGCTGGTGATGATGGT+GGG | + | chr4.3:72142759-72142778 | None:intergenic | 50.0% |
| GCCCCTGCACAAATTATCCC+TGG | - | chr4.3:72142106-72142125 | MS.gene48885:CDS | 55.0% | |
| ! | GGATAATTTGTGCAGGGGCC+AGG | + | chr4.3:72142105-72142124 | None:intergenic | 55.0% |
| ! | GTGGAGAAGTGTGGAATCCG+GGG | - | chr4.3:72142073-72142092 | MS.gene48885:CDS | 55.0% |
| !! | GATGATGGTGGGTTAGGAGG+AGG | + | chr4.3:72142748-72142767 | None:intergenic | 55.0% |
| !! | GCTGGTGATGATGGTGGGTT+AGG | + | chr4.3:72142754-72142773 | None:intergenic | 55.0% |
| !! | GCTTGAGCTGGTGATGATGG+TGG | + | chr4.3:72142760-72142779 | None:intergenic | 55.0% |
| !! | GGTGATGATGGTGGGTTAGG+AGG | + | chr4.3:72142751-72142770 | None:intergenic | 55.0% |
| TGGAATCCGGGGTTCGACCC+TGG | - | chr4.3:72142084-72142103 | MS.gene48885:CDS | 65.0% | |
| CAGGGGCCAGGGTCGAACCC+CGG | + | chr4.3:72142093-72142112 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 72141310 | 72143364 | 72141310 | ID=MS.gene48885 |
| chr4.3 | mRNA | 72141310 | 72143364 | 72141310 | ID=MS.gene48885.t1;Parent=MS.gene48885 |
| chr4.3 | exon | 72143277 | 72143364 | 72143277 | ID=MS.gene48885.t1.exon1;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72143277 | 72143364 | 72143277 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72143065 | 72143135 | 72143065 | ID=MS.gene48885.t1.exon2;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72143065 | 72143135 | 72143065 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72142894 | 72142975 | 72142894 | ID=MS.gene48885.t1.exon3;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72142894 | 72142975 | 72142894 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72142066 | 72142181 | 72142066 | ID=MS.gene48885.t1.exon4;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72142066 | 72142181 | 72142066 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72141867 | 72141989 | 72141867 | ID=MS.gene48885.t1.exon5;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72141867 | 72141989 | 72141867 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72141570 | 72141647 | 72141570 | ID=MS.gene48885.t1.exon6;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72141570 | 72141647 | 72141570 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| chr4.3 | exon | 72141310 | 72141312 | 72141310 | ID=MS.gene48885.t1.exon7;Parent=MS.gene48885.t1 |
| chr4.3 | CDS | 72141310 | 72141312 | 72141310 | ID=cds.MS.gene48885.t1;Parent=MS.gene48885.t1 |
| Gene Sequence |
| Protein sequence |
