AlfalfaGEDB | MS.gene39534

M. sativa cultivar XinJiangDaYe / MS.gene39534

KEGG: K22588
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0008168 GO:0008171 GO:0016740 GO:0016741 GO:0046983
PFAM: PF00891.21 PF08100.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39534.t1	XP_013456930.1	98.6	364	4	1	1	363	1	364	3.80E-203	717.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39534.t1	Q29U70	98.6	364	4	1	1	363	1	364	9.1e-206	717.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39534.t1	A0A072UNK8	98.6	364	4	1	1	363	1	364	2.7e-203	717.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05015	MS.gene39534	0.867082	1.78E-65	-1.69E-46
MS.gene050381	MS.gene39534	0.821082	4.88E-53	-1.69E-46
MS.gene050496	MS.gene39534	-0.801333	9.46E-49	-1.69E-46
MS.gene050883	MS.gene39534	0.820079	8.29E-53	-1.69E-46
MS.gene051044	MS.gene39534	0.828681	7.85E-55	-1.69E-46
MS.gene051127	MS.gene39534	0.857367	1.71E-62	-1.69E-46
MS.gene051138	MS.gene39534	0.84458	6.92E-59	-1.69E-46
MS.gene051341	MS.gene39534	0.816671	4.90E-52	-1.69E-46
MS.gene051366	MS.gene39534	0.801337	9.44E-49	-1.69E-46
MS.gene051422	MS.gene39534	0.807464	4.99E-50	-1.69E-46
MS.gene052089	MS.gene39534	0.808087	3.68E-50	-1.69E-46
MS.gene052566	MS.gene39534	0.816642	4.98E-52	-1.69E-46
MS.gene052802	MS.gene39534	0.809112	2.22E-50	-1.69E-46
MS.gene053420	MS.gene39534	0.824962	6.08E-54	-1.69E-46
MS.gene053488	MS.gene39534	0.81231	4.52E-51	-1.69E-46
MS.gene053561	MS.gene39534	0.824767	6.75E-54	-1.69E-46
MS.gene053887	MS.gene39534	0.806111	9.64E-50	-1.69E-46
MS.gene054056	MS.gene39534	0.830213	3.33E-55	-1.69E-46
MS.gene055124	MS.gene39534	0.812596	3.91E-51	-1.69E-46
MS.gene055154	MS.gene39534	0.800656	1.30E-48	-1.69E-46
MS.gene055942	MS.gene39534	0.881721	1.90E-70	-1.69E-46
MS.gene056224	MS.gene39534	0.800552	1.37E-48	-1.69E-46
MS.gene056311	MS.gene39534	0.810954	8.92E-51	-1.69E-46
MS.gene056504	MS.gene39534	-0.800286	1.55E-48	-1.69E-46
MS.gene056856	MS.gene39534	0.831708	1.43E-55	-1.69E-46
MS.gene056857	MS.gene39534	0.809539	1.80E-50	-1.69E-46
MS.gene057548	MS.gene39534	0.823665	1.23E-53	-1.69E-46
MS.gene057549	MS.gene39534	0.824666	7.13E-54	-1.69E-46
MS.gene057768	MS.gene39534	0.817143	3.84E-52	-1.69E-46
MS.gene058068	MS.gene39534	0.807067	6.06E-50	-1.69E-46
MS.gene058099	MS.gene39534	0.846053	2.77E-59	-1.69E-46
MS.gene058109	MS.gene39534	0.825921	3.60E-54	-1.69E-46
MS.gene058111	MS.gene39534	0.830443	2.93E-55	-1.69E-46
MS.gene058171	MS.gene39534	0.851697	7.49E-61	-1.69E-46
MS.gene058173	MS.gene39534	0.848116	7.52E-60	-1.69E-46
MS.gene058175	MS.gene39534	0.859917	2.97E-63	-1.69E-46
MS.gene058268	MS.gene39534	0.810871	9.29E-51	-1.69E-46
MS.gene058493	MS.gene39534	0.803159	3.98E-49	-1.69E-46
MS.gene058628	MS.gene39534	0.838451	2.85E-57	-1.69E-46
MS.gene058629	MS.gene39534	0.825467	4.61E-54	-1.69E-46
MS.gene058630	MS.gene39534	0.831542	1.57E-55	-1.69E-46
MS.gene059045	MS.gene39534	0.814598	1.42E-51	-1.69E-46
MS.gene059690	MS.gene39534	0.814631	1.40E-51	-1.69E-46
MS.gene059694	MS.gene39534	0.808155	3.56E-50	-1.69E-46
MS.gene059697	MS.gene39534	0.807642	4.57E-50	-1.69E-46
MS.gene061044	MS.gene39534	0.812417	4.28E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39534.t1	MTR_4g088190	98.626	364	4	1	1	363	1	364	0.0	731
MS.gene39534.t1	MTR_4g088200	88.981	363	38	1	1	363	1	361	0.0	664
MS.gene39534.t1	MTR_4g033015	75.275	364	88	2	1	363	1	363	0.0	544
MS.gene39534.t1	MTR_4g033200	75.000	364	89	2	1	363	1	363	0.0	541
MS.gene39534.t1	MTR_4g033150	74.451	364	91	2	1	363	1	363	0.0	540
MS.gene39534.t1	MTR_4g033170	74.931	363	85	1	1	363	1	357	0.0	525
MS.gene39534.t1	MTR_4g033085	75.207	363	84	1	1	363	1	357	0.0	510
MS.gene39534.t1	MTR_0794s0020	73.200	250	65	2	1	249	1	249	2.22e-133	381
MS.gene39534.t1	MTR_7g014570	49.862	363	171	5	1	363	1	352	1.26e-121	355
MS.gene39534.t1	MTR_7g014510	49.587	363	177	4	1	363	1	357	1.80e-121	355
MS.gene39534.t1	MTR_5g074600	49.577	355	171	5	11	363	11	359	3.23e-120	352
MS.gene39534.t1	MTR_4g088345	52.198	364	165	5	1	363	1	356	6.88e-119	348
MS.gene39534.t1	MTR_7g024500	48.596	356	171	5	6	361	5	348	3.90e-117	343
MS.gene39534.t1	MTR_5g074680	47.740	354	177	5	11	362	12	359	1.62e-116	342
MS.gene39534.t1	MTR_1g076940	48.907	366	179	6	1	363	1	361	1.10e-115	340
MS.gene39534.t1	MTR_7g024520	49.719	356	167	5	6	361	5	348	7.68e-115	338
MS.gene39534.t1	MTR_5g074580	48.451	355	175	5	11	363	12	360	1.17e-114	337
MS.gene39534.t1	MTR_4g079160	46.328	354	181	3	11	363	10	355	3.45e-107	318
MS.gene39534.t1	MTR_4g088340	51.026	341	158	5	24	363	1	333	6.60e-107	317
MS.gene39534.t1	MTR_5g074610	45.609	353	159	3	11	363	7	326	8.14e-106	314
MS.gene39534.t1	MTR_5g074540	45.070	355	169	5	11	363	11	341	9.64e-105	311
MS.gene39534.t1	MTR_4g038440	34.060	367	226	9	2	363	13	368	4.17e-61	201
MS.gene39534.t1	MTR_3g083620	36.152	343	204	9	20	359	27	357	1.73e-60	199
MS.gene39534.t1	MTR_0017s0100	33.880	366	228	8	2	363	13	368	2.59e-59	196
MS.gene39534.t1	MTR_0079s0070	34.211	342	209	9	27	363	4	334	5.65e-57	189
MS.gene39534.t1	MTR_1g076930	41.975	243	132	5	24	263	1	237	1.52e-55	182
MS.gene39534.t1	MTR_1g036460	31.487	343	211	10	33	362	38	369	4.83e-43	153
MS.gene39534.t1	MTR_1g036490	31.858	339	215	8	33	362	38	369	9.93e-43	152
MS.gene39534.t1	MTR_5g098170	33.837	331	201	10	28	350	30	350	1.53e-42	152
MS.gene39534.t1	MTR_7g012090	31.343	335	213	8	26	350	34	361	2.55e-41	149
MS.gene39534.t1	MTR_3g092900	32.203	354	206	11	9	349	17	349	1.34e-40	147
MS.gene39534.t1	MTR_1g096310	31.233	365	226	10	4	358	16	365	4.19e-40	145
MS.gene39534.t1	MTR_7g012070	30.149	335	217	8	26	350	34	361	1.22e-39	144
MS.gene39534.t1	MTR_2g072790	33.141	347	204	11	26	360	21	351	1.42e-39	144
MS.gene39534.t1	MTR_7g012040	30.699	329	217	7	26	350	33	354	2.12e-39	144
MS.gene39534.t1	MTR_8g068750	31.429	350	216	10	9	348	27	362	2.14e-39	144
MS.gene39534.t1	MTR_7g011990	28.736	348	222	9	26	362	34	366	7.70e-39	142
MS.gene39534.t1	MTR_7g118320	32.733	333	203	13	26	348	26	347	9.20e-39	142
MS.gene39534.t1	MTR_7g011900	30.909	330	216	7	26	350	34	356	1.28e-38	141
MS.gene39534.t1	MTR_8g068690	32.515	326	205	9	26	344	40	357	2.85e-38	140
MS.gene39534.t1	MTR_0591s0010	31.288	326	209	9	26	344	41	358	9.54e-38	139
MS.gene39534.t1	MTR_8g068870	30.141	355	230	10	4	348	2	348	5.02e-37	137
MS.gene39534.t1	MTR_2g055940	31.269	323	207	10	32	348	34	347	2.59e-36	135
MS.gene39534.t1	MTR_7g118300	30.499	341	212	10	26	355	42	368	9.40e-36	134
MS.gene39534.t1	MTR_4g038440	32.707	266	167	6	2	263	13	270	1.27e-34	128
MS.gene39534.t1	MTR_7g012020	29.091	330	222	7	26	350	33	355	4.67e-34	129
MS.gene39534.t1	MTR_8g024160	40.000	185	106	3	173	355	140	321	6.32e-34	128
MS.gene39534.t1	MTR_1g036510	29.904	311	207	7	56	362	80	383	1.40e-33	128
MS.gene39534.t1	MTR_3g021430	30.618	356	218	11	13	358	14	350	3.80e-33	126
MS.gene39534.t1	MTR_3g021440	31.250	336	199	14	32	353	42	359	1.12e-32	125
MS.gene39534.t1	MTR_3g096050	28.909	339	221	11	32	362	31	357	1.95e-31	122
MS.gene39534.t1	MTR_8g068570	28.662	314	178	8	27	331	42	318	2.45e-29	115
MS.gene39534.t1	MTR_6g078850	27.874	348	231	9	9	348	33	368	9.60e-29	117
MS.gene39534.t1	MTR_8g068730	28.286	350	195	10	9	348	27	330	5.17e-28	112
MS.gene39534.t1	MTR_3g096070	26.488	336	206	10	32	360	31	332	1.58e-24	102
MS.gene39534.t1	MTR_7g012020	29.555	247	165	6	26	267	33	275	7.36e-23	97.4
MS.gene39534.t1	MTR_1g050220	33.117	154	90	2	194	347	147	287	6.15e-20	89.4
MS.gene39534.t1	MTR_8g068690	28.745	247	165	7	26	266	40	281	6.46e-19	86.3
MS.gene39534.t1	MTR_3g096050	29.876	241	154	9	32	266	31	262	6.02e-17	80.1
MS.gene39534.t1	MTR_8g068740	27.076	277	153	7	26	293	41	277	1.18e-16	79.7
MS.gene39534.t1	MTR_1g036510	29.907	214	136	7	56	263	80	285	8.91e-16	77.4
MS.gene39534.t1	MTR_8g068600	26.482	253	156	10	38	276	27	263	5.94e-15	74.7
MS.gene39534.t1	MTR_7g012020	29.630	216	143	6	26	236	33	244	8.77e-15	73.9
MS.gene39534.t1	MTR_4g025570	45.783	83	38	3	6	88	16	91	1.58e-14	72.4
MS.gene39534.t1	MTR_4g025570	38.542	96	55	3	265	359	122	214	2.09e-12	66.2
MS.gene39534.t1	MTR_8g068620	36.170	94	60	0	173	266	20	113	2.14e-13	66.6
MS.gene39534.t1	MTR_8g068760	41.379	87	47	2	263	348	135	218	4.68e-12	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39534.t1	AT4G35150	33.240	358	179	11	16	362	15	323	5.74e-50	170
MS.gene39534.t1	AT4G35160	34.160	363	212	11	16	362	29	380	2.83e-46	162
MS.gene39534.t1	AT5G54160	33.718	347	199	9	28	361	31	359	4.07e-44	156
MS.gene39534.t1	AT3G53140	32.286	350	208	13	26	360	21	356	1.09e-39	144
MS.gene39534.t1	AT1G51990	31.649	376	222	14	4	363	6	362	1.89e-38	141
MS.gene39534.t1	AT1G51990	30.343	379	223	12	4	363	6	362	2.58e-38	140
MS.gene39534.t1	AT1G77520	31.283	374	224	13	9	362	20	380	2.79e-38	141
MS.gene39534.t1	AT5G53810	31.624	351	212	12	29	362	38	377	3.40e-34	130
MS.gene39534.t1	AT1G77530	33.062	369	224	13	9	362	20	380	1.73e-33	128
MS.gene39534.t1	AT1G33030	30.409	342	221	10	28	362	18	349	3.98e-33	126
MS.gene39534.t1	AT1G63140	29.032	372	236	12	9	362	39	400	1.04e-31	124
MS.gene39534.t1	AT1G63140	29.032	372	236	12	9	362	19	380	1.07e-31	123
MS.gene39534.t1	AT1G62900	35.211	213	119	6	153	362	8	204	1.06e-24	100
MS.gene39534.t1	AT1G76790	27.654	358	219	12	7	344	9	346	2.47e-24	102
MS.gene39534.t1	AT5G37170	30.412	194	129	4	172	362	43	233	1.32e-22	95.5
MS.gene39534.t1	AT5G37170	30.412	194	129	4	172	362	143	333	2.70e-22	96.7
MS.gene39534.t1	AT5G37170	31.111	180	115	5	187	360	7	183	1.68e-21	91.3
MS.gene39534.t1	AT1G21130	26.801	347	201	15	31	350	40	360	1.31e-20	92.4
MS.gene39534.t1	AT1G21100	25.872	344	208	13	31	350	40	360	1.10e-19	89.7
MS.gene39534.t1	AT1G21110	25.731	342	201	14	31	345	40	355	1.16e-19	89.7
MS.gene39534.t1	AT1G21120	25.291	344	210	12	31	350	40	360	2.83e-19	88.6
MS.gene39534.t1	AT1G21120	25.291	344	210	12	31	350	88	408	3.98e-19	88.6
MS.gene39534.t1	AT1G63140	27.306	271	174	9	9	263	39	302	8.95e-15	74.7
MS.gene39534.t1	AT1G33030	28.099	242	161	8	28	263	18	252	6.98e-13	68.6
MS.gene39534.t1	AT1G33030	28.033	239	159	8	31	263	21	252	1.09e-12	68.2

Find 65 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGCAACAGGGGAGAGCTTC+TGG	0.191292	4.2:-23149674	MS.gene39534:CDS
GCTGTTCTTCAATATATTTA+AGG	0.205575	4.2:+23148996	None:intergenic
TTTGAAACTTCATCCTTCTA+AGG	0.260291	4.2:-23149949	MS.gene39534:CDS
CGAACACGTGTTTGTTCTCT+TGG	0.272286	4.2:+23149562	None:intergenic
TGTTTATCTTCAATTGTTAA+AGG	0.290462	4.2:-23149780	MS.gene39534:CDS
CAGGTTGTTGGTAACTTAAC+TGG	0.291153	4.2:-23149435	MS.gene39534:CDS
TACAGTGGGTTCTGCATGAT+TGG	0.326455	4.2:-23149032	MS.gene39534:intron
AGAAACCGTTGTGTGTTAAA+AGG	0.336053	4.2:+23149901	None:intergenic
TGTATACATGTTTGTACAAA+TGG	0.340652	4.2:+23150075	None:intergenic
CAAACACGTGTTCGAGGGTT+TGG	0.341667	4.2:-23149553	MS.gene39534:CDS
ACACTGTTTGAGTGTGCAAC+AGG	0.344866	4.2:-23149687	MS.gene39534:CDS
GTGATGCTTACAATGTTTCT+TGG	0.364082	4.2:-23148859	MS.gene39534:CDS
TTCATTGAACCATTTCTTTG+AGG	0.375008	4.2:+23149722	None:intergenic
TGGATTAACTGAACTGCAAT+TGG	0.377228	4.2:-23148893	MS.gene39534:CDS
AATGAAAATTTGAACTTTGT+TGG	0.379769	4.2:-23149411	MS.gene39534:CDS
GCTAATTCAGAAAGAGTCAT+TGG	0.394069	4.2:+23149978	None:intergenic
CCACTTATGAGAAGCTTTGA+AGG	0.396791	4.2:+23149813	None:intergenic
GTTGATGTTGGAGGTGGTAC+TGG	0.401447	4.2:-23149522	MS.gene39534:CDS
TTGGAGTCTCTTGTTGATGT+TGG	0.404723	4.2:-23149534	MS.gene39534:CDS
CTCCACATGTCTAAAGAACT+TGG	0.410016	4.2:+23149747	None:intergenic
TGAAGTATATTAACCTTAGA+AGG	0.411294	4.2:+23149936	None:intergenic
AAGAAAGAACAAAGCAAGAA+TGG	0.426132	4.2:-23148834	MS.gene39534:CDS
ACTGCAATTGGACTATGATT+TGG	0.427028	4.2:-23148881	MS.gene39534:CDS
GAGTGACTTGAAGCCAGAAA+TGG	0.427969	4.2:+23148765	None:intergenic
CCTTCAAAGCTTCTCATAAG+TGG	0.434600	4.2:-23149813	MS.gene39534:CDS
AGTGACTTGAAGCCAGAAAT+GGG	0.443496	4.2:+23148766	None:intergenic
TGTGGAGTTCCTCAAAGAAA+TGG	0.466565	4.2:-23149731	MS.gene39534:CDS
TGCAACAGGGGAGAGCTTCT+GGG	0.468202	4.2:-23149673	MS.gene39534:CDS
GTGTTAAAAGGCGCAAGAAT+CGG	0.481292	4.2:+23149913	None:intergenic
GAAAATTTGAACTTTGTTGG+TGG	0.485249	4.2:-23149408	MS.gene39534:CDS
TTCACTTTCTTCAGAACCAT+TGG	0.487182	4.2:+23150115	None:intergenic
TTTGATCAACCACAGGTTGT+TGG	0.492105	4.2:-23149447	MS.gene39534:CDS
CTCAAATCAGCCATGGAACT+AGG	0.496080	4.2:-23150032	MS.gene39534:CDS
GAGTCTCTTGTTGATGTTGG+AGG	0.496314	4.2:-23149531	MS.gene39534:CDS
ATCCAAGTTCTTTAGACATG+TGG	0.496638	4.2:-23149749	MS.gene39534:CDS
GAGTATGTTTCAAGATGCTA+TGG	0.498973	4.2:-23149616	MS.gene39534:CDS
ACAATAGTGAAAGGCAAAGA+AGG	0.501253	4.2:-23149870	MS.gene39534:CDS
ATCAGCTATGCCTAGTTCCA+TGG	0.501316	4.2:+23150022	None:intergenic
GAAATGGTTCAATGAAGATA+AGG	0.502299	4.2:-23149715	MS.gene39534:CDS
AGAAAGAACAAAGCAAGAAT+GGG	0.507206	4.2:-23148833	MS.gene39534:CDS
ACAATTGAAGATAAACATGT+TGG	0.521606	4.2:+23149786	None:intergenic
ATACAACTTTGTTAGTTCCA+TGG	0.535416	4.2:-23150057	MS.gene39534:CDS
GAAATGGCTTTCACTACCAA+TGG	0.538698	4.2:-23150131	MS.gene39534:CDS
AGAGAACAAACACGTGTTCG+AGG	0.543577	4.2:-23149559	MS.gene39534:CDS
ATTTCACACAAAGGAAAGGA+TGG	0.547784	4.2:-23148964	MS.gene39534:CDS
AGTAAAACAGCATCAGCAGA+AGG	0.551999	4.2:+23149366	None:intergenic
TCTCTTGTTGATGTTGGAGG+TGG	0.554073	4.2:-23149528	MS.gene39534:CDS
AGGATGAAGTTTCAAAGAAG+AGG	0.557817	4.2:+23149956	None:intergenic
CATGGCTGATTTGAGTGCCA+TGG	0.563511	4.2:+23150040	None:intergenic
AGCAATTTCACACAAAGGAA+AGG	0.577136	4.2:-23148968	MS.gene39534:CDS
GAGAACAAACACGTGTTCGA+GGG	0.583061	4.2:-23149558	MS.gene39534:CDS
CACTGTTTGAGTGTGCAACA+GGG	0.603315	4.2:-23149686	MS.gene39534:CDS
CATGGCACTCAAATCAGCCA+TGG	0.607444	4.2:-23150039	MS.gene39534:CDS
GATGTTGGAGGTGGTACTGG+TGG	0.610342	4.2:-23149519	MS.gene39534:CDS
TGTACAAATGGATTTGAGCA+TGG	0.612036	4.2:+23150087	None:intergenic
AAAACAGTGCATTTAAGGTG+AGG	0.613644	4.2:+23149468	None:intergenic
GTAAAACAGCATCAGCAGAA+GGG	0.623492	4.2:+23149367	None:intergenic
AAAGAAGCAATTTCACACAA+AGG	0.626707	4.2:-23148973	MS.gene39534:CDS
GCTGATGCAATTCACAACCA+TGG	0.632132	4.2:-23150005	MS.gene39534:CDS
GTGACTTGAAGCCAGAAATG+GGG	0.636393	4.2:+23148767	None:intergenic
TGACTTGAAGCCAGAAATGG+GGG	0.646680	4.2:+23148768	None:intergenic
ACTGTTTGAGTGTGCAACAG+GGG	0.649035	4.2:-23149685	MS.gene39534:CDS
GATGAAACAAGTGACGATCG+TGG	0.671140	4.2:-23148913	MS.gene39534:CDS
CTTATGAGAAGCTTTGAAGG+AGG	0.681784	4.2:+23149816	None:intergenic
GTTAAGTTACCAACAACCTG+TGG	0.693381	4.2:+23149438	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATAACTTTTACTAATA+AGG	-	chr4.2:23149736-23149755	MS.gene39534:CDS	10.0%
!!!	AATTGTTACTATTTCAATTT+TGG	-	chr4.2:23149551-23149570	MS.gene39534:CDS	15.0%
!!!	TGACATTTAATTTAGTTATT+TGG	+	chr4.2:23149811-23149830	None:intergenic	15.0%
!!!	TTTTACTAATAAGGATAAAA+TGG	-	chr4.2:23149745-23149764	MS.gene39534:CDS	15.0%
!!	AAGTTATATAACAAGATTGA+AGG	+	chr4.2:23149727-23149746	None:intergenic	20.0%
!!	AATGAAAATTTGAACTTTGT+TGG	-	chr4.2:23149464-23149483	MS.gene39534:CDS	20.0%
!!	TGTTTATCTTCAATTGTTAA+AGG	-	chr4.2:23149095-23149114	MS.gene39534:intron	20.0%
!!!	ACAAATTAAGATGCATATTT+TGG	-	chr4.2:23149780-23149799	MS.gene39534:CDS	20.0%
!	ACAATTGAAGATAAACATGT+TGG	+	chr4.2:23149092-23149111	None:intergenic	25.0%
!	GCTGTTCTTCAATATATTTA+AGG	+	chr4.2:23149882-23149901	None:intergenic	25.0%
!	TGAAGTATATTAACCTTAGA+AGG	+	chr4.2:23148942-23148961	None:intergenic	25.0%
!	TGTATACATGTTTGTACAAA+TGG	+	chr4.2:23148803-23148822	None:intergenic	25.0%
!	TGTTAGACATTAAAATGTGT+AGG	-	chr4.2:23149693-23149712	MS.gene39534:CDS	25.0%
!	TTTGCAAAAACAATAGTGAA+AGG	-	chr4.2:23148996-23149015	MS.gene39534:CDS	25.0%
!!	CAATTGTCTTTTATTACAGT+GGG	-	chr4.2:23149829-23149848	MS.gene39534:CDS	25.0%
!!	TCAATTGTCTTTTATTACAG+TGG	-	chr4.2:23149828-23149847	MS.gene39534:CDS	25.0%
!!	TCGTTGACTAAATTTAATTC+TGG	+	chr4.2:23149633-23149652	None:intergenic	25.0%
	AAAGAAGCAATTTCACACAA+AGG	-	chr4.2:23149902-23149921	MS.gene39534:CDS	30.0%
	AAGAAAGAACAAAGCAAGAA+TGG	-	chr4.2:23150041-23150060	MS.gene39534:CDS	30.0%
	AGAAAGAACAAAGCAAGAAT+GGG	-	chr4.2:23150042-23150061	MS.gene39534:CDS	30.0%
	ATACAACTTTGTTAGTTCCA+TGG	-	chr4.2:23148818-23148837	MS.gene39534:CDS	30.0%
	GAAAATTTGAACTTTGTTGG+TGG	-	chr4.2:23149467-23149486	MS.gene39534:CDS	30.0%
	GAAATGGTTCAATGAAGATA+AGG	-	chr4.2:23149160-23149179	MS.gene39534:intron	30.0%
	GATCAAAAACAGTGCATTTA+AGG	+	chr4.2:23149415-23149434	None:intergenic	30.0%
	TTCATTGAACCATTTCTTTG+AGG	+	chr4.2:23149156-23149175	None:intergenic	30.0%
!	TTTGAAACTTCATCCTTCTA+AGG	-	chr4.2:23148926-23148945	MS.gene39534:CDS	30.0%
	ACAATAGTGAAAGGCAAAGA+AGG	-	chr4.2:23149005-23149024	MS.gene39534:CDS	35.0%
	ACTGCAATTGGACTATGATT+TGG	-	chr4.2:23149994-23150013	MS.gene39534:CDS	35.0%
	AGAAACCGTTGTGTGTTAAA+AGG	+	chr4.2:23148977-23148996	None:intergenic	35.0%
	AGCAATTTCACACAAAGGAA+AGG	-	chr4.2:23149907-23149926	MS.gene39534:CDS	35.0%
	AGGATGAAGTTTCAAAGAAG+AGG	+	chr4.2:23148922-23148941	None:intergenic	35.0%
	ATCCAAGTTCTTTAGACATG+TGG	-	chr4.2:23149126-23149145	MS.gene39534:intron	35.0%
	ATTTCACACAAAGGAAAGGA+TGG	-	chr4.2:23149911-23149930	MS.gene39534:CDS	35.0%
	GAAAAACTCATATACGATGC+AGG	-	chr4.2:23150064-23150083	MS.gene39534:CDS	35.0%
	GAGTATGTTTCAAGATGCTA+TGG	-	chr4.2:23149259-23149278	MS.gene39534:intron	35.0%
	GCTAATTCAGAAAGAGTCAT+TGG	+	chr4.2:23148900-23148919	None:intergenic	35.0%
	GTCATTGTCAATATTGTGTC+CGG	+	chr4.2:23149603-23149622	None:intergenic	35.0%
	GTGATGCTTACAATGTTTCT+TGG	-	chr4.2:23150016-23150035	MS.gene39534:CDS	35.0%
	TACAAAATTACCCCCATTTC+TGG	-	chr4.2:23150097-23150116	MS.gene39534:CDS	35.0%
	TGGATTAACTGAACTGCAAT+TGG	-	chr4.2:23149982-23150001	MS.gene39534:CDS	35.0%
	TTCACTTTCTTCAGAACCAT+TGG	+	chr4.2:23148763-23148782	None:intergenic	35.0%
!	AAAACAGTGCATTTAAGGTG+AGG	+	chr4.2:23149410-23149429	None:intergenic	35.0%
!	TGTACAAATGGATTTGAGCA+TGG	+	chr4.2:23148791-23148810	None:intergenic	35.0%
!!	CAATTTTGGTCTATGAAGCA+CGG	-	chr4.2:23149565-23149584	MS.gene39534:CDS	35.0%
!!!	AAAGAGTCATTGGTTTTCCA+TGG	+	chr4.2:23148890-23148909	None:intergenic	35.0%
!!!	TGCTGATGCTGTTTTACTTA+AGG	-	chr4.2:23149514-23149533	MS.gene39534:CDS	35.0%
	AGTAAAACAGCATCAGCAGA+AGG	+	chr4.2:23149512-23149531	None:intergenic	40.0%
	AGTGACTTGAAGCCAGAAAT+GGG	+	chr4.2:23150112-23150131	None:intergenic	40.0%
	CCTTCAAAGCTTCTCATAAG+TGG	-	chr4.2:23149062-23149081	MS.gene39534:intron	40.0%
	CTCCACATGTCTAAAGAACT+TGG	+	chr4.2:23149131-23149150	None:intergenic	40.0%
	GTAAAACAGCATCAGCAGAA+GGG	+	chr4.2:23149511-23149530	None:intergenic	40.0%
	GTTAAGTTACCAACAACCTG+TGG	+	chr4.2:23149440-23149459	None:intergenic	40.0%
	TGTGGAGTTCCTCAAAGAAA+TGG	-	chr4.2:23149144-23149163	MS.gene39534:intron	40.0%
	TTGGAGTCTCTTGTTGATGT+TGG	-	chr4.2:23149341-23149360	MS.gene39534:intron	40.0%
	TTTGATCAACCACAGGTTGT+TGG	-	chr4.2:23149428-23149447	MS.gene39534:CDS	40.0%
!	CCACTTATGAGAAGCTTTGA+AGG	+	chr4.2:23149065-23149084	None:intergenic	40.0%
!	GTGTTAAAAGGCGCAAGAAT+CGG	+	chr4.2:23148965-23148984	None:intergenic	40.0%
!	TTGCGCCTTTTAACACACAA+CGG	-	chr4.2:23148969-23148988	MS.gene39534:CDS	40.0%
!!	CAGGTTGTTGGTAACTTAAC+TGG	-	chr4.2:23149440-23149459	MS.gene39534:CDS	40.0%
!!	CTTATGAGAAGCTTTGAAGG+AGG	+	chr4.2:23149062-23149081	None:intergenic	40.0%
!!!	CACTGTTTTTGATCAACCAC+AGG	-	chr4.2:23149421-23149440	MS.gene39534:CDS	40.0%
	ACACTGTTTGAGTGTGCAAC+AGG	-	chr4.2:23149188-23149207	MS.gene39534:intron	45.0%
	ACTGTTTGAGTGTGCAACAG+GGG	-	chr4.2:23149190-23149209	MS.gene39534:intron	45.0%
	AGAGAACAAACACGTGTTCG+AGG	-	chr4.2:23149316-23149335	MS.gene39534:intron	45.0%
	ATCAGCTATGCCTAGTTCCA+TGG	+	chr4.2:23148856-23148875	None:intergenic	45.0%
	CACTGTTTGAGTGTGCAACA+GGG	-	chr4.2:23149189-23149208	MS.gene39534:intron	45.0%
	CGAACACGTGTTTGTTCTCT+TGG	+	chr4.2:23149316-23149335	None:intergenic	45.0%
	CTCAAATCAGCCATGGAACT+AGG	-	chr4.2:23148843-23148862	MS.gene39534:CDS	45.0%
	GAGAACAAACACGTGTTCGA+GGG	-	chr4.2:23149317-23149336	MS.gene39534:intron	45.0%
	GAGTCTCTTGTTGATGTTGG+AGG	-	chr4.2:23149344-23149363	MS.gene39534:intron	45.0%
	GAGTGACTTGAAGCCAGAAA+TGG	+	chr4.2:23150113-23150132	None:intergenic	45.0%
	GATGAAACAAGTGACGATCG+TGG	-	chr4.2:23149962-23149981	MS.gene39534:CDS	45.0%
	GCTGATGCAATTCACAACCA+TGG	-	chr4.2:23148870-23148889	MS.gene39534:CDS	45.0%
	GTGACTTGAAGCCAGAAATG+GGG	+	chr4.2:23150111-23150130	None:intergenic	45.0%
	TCTCTTGTTGATGTTGGAGG+TGG	-	chr4.2:23149347-23149366	MS.gene39534:intron	45.0%
	TGACTTGAAGCCAGAAATGG+GGG	+	chr4.2:23150110-23150129	None:intergenic	45.0%
!	TACAGTGGGTTCTGCATGAT+TGG	-	chr4.2:23149843-23149862	MS.gene39534:CDS	45.0%
	CAAACACGTGTTCGAGGGTT+TGG	-	chr4.2:23149322-23149341	MS.gene39534:intron	50.0%
	CATGGCACTCAAATCAGCCA+TGG	-	chr4.2:23148836-23148855	MS.gene39534:CDS	50.0%
	GTTGATGTTGGAGGTGGTAC+TGG	-	chr4.2:23149353-23149372	MS.gene39534:intron	50.0%
!!	CATGGCTGATTTGAGTGCCA+TGG	+	chr4.2:23148838-23148857	None:intergenic	50.0%
	TGCAACAGGGGAGAGCTTCT+GGG	-	chr4.2:23149202-23149221	MS.gene39534:intron	55.0%
!	GATGTTGGAGGTGGTACTGG+TGG	-	chr4.2:23149356-23149375	MS.gene39534:intron	55.0%
	AGCACGGACACTGCACACAC+CGG	-	chr4.2:23149581-23149600	MS.gene39534:CDS	60.0%
	GTGCAACAGGGGAGAGCTTC+TGG	-	chr4.2:23149201-23149220	MS.gene39534:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	23148747	23150150	23148747	ID=MS.gene39534
chr4.2	mRNA	23148747	23150150	23148747	ID=MS.gene39534.t1;Parent=MS.gene39534
chr4.2	exon	23149362	23150150	23149362	ID=MS.gene39534.t1.exon1;Parent=MS.gene39534.t1
chr4.2	CDS	23149362	23150150	23149362	ID=cds.MS.gene39534.t1;Parent=MS.gene39534.t1
chr4.2	exon	23148747	23149049	23148747	ID=MS.gene39534.t1.exon2;Parent=MS.gene39534.t1
chr4.2	CDS	23148747	23149049	23148747	ID=cds.MS.gene39534.t1;Parent=MS.gene39534.t1

Gene Sequence

>MS.gene39534

ATGGCTTTCACTACCAATGGTTCTGAAGAAAGTGAACTGTACCATGCTCAAATCCATTTGTACAAACATGTATACAACTTTGTTAGTTCCATGGCACTCAAATCAGCCATGGAACTAGGCATAGCTGATGCAATTCACAACCATGGAAAACCAATGACTCTTTCTGAATTAGCCTCTTCTTTGAAACTTCATCCTTCTAAGGTTAATATACTTCACCGATTCTTGCGCCTTTTAACACACAACGGTTTCTTTGCAAAAACAATAGTGAAAGGCAAAGAAGGAGAAGAAGAAGAAATAGCATATTCTCTCACACCTCCTTCAAAGCTTCTCATAAGTGGAAAACCAACATGTTTATCTTCAATTGTTAAAGGAGCACTTCATCCAAGTTCTTTAGACATGTGGAGTTCCTCAAAGAAATGGTTCAATGAAGATAAGGAACAAACACTGTTTGAGTGTGCAACAGGGGAGAGCTTCTGGGATTTTCTTAACAAAGATTCTGAATCTAGTACTTTGAGTATGTTTCAAGATGCTATGGCATCTGATTCTAGAATGTTTAAGCTTGTTCTCCAAGAGAACAAACACGTGTTCGAGGGTTTGGAGTCTCTTGTTGATGTTGGAGGTGGTACTGGTGGTGTCACAAAACTCATTAATGAAATTTTTCCTCACCTTAAATGCACTGTTTTTGATCAACCACAGGTTGTTGGTAACTTAACTGGAAATGAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTTCTGCTGATGCTGTTTTACTTAAGGTATGCATGTTCAACAATTGTTACTATTTCAATTTTGGTCTATGAAGCACGGACACTGCACACACCGGACACAATATTGACAATGACATAGACACCAGAATTAAATTTAGTCAACGAAACTGTTTGAATTTAATAACATATGACTGCGTCGAGATAATGTTAGACATTAAAATGTGTAGGACACATTACCTTCAATCTTGTTATATAACTTTTACTAATAAGGATAAAATGGTAGTAACTAGTAACAAATTAAGATGCATATTTTGGATCAACCAAATAACTAAATTAAATGTCAATTGTCTTTTATTACAGTGGGTTCTGCATGATTGGAATGATGAACAATCCTTAAATATATTGAAGAACAGCAAAGAAGCAATTTCACACAAAGGAAAGGATGGAAAAGTGATTATCATCGATATATCAATTGATGAAACAAGTGACGATCGTGGATTAACTGAACTGCAATTGGACTATGATTTGGTGATGCTTACAATGTTTCTTGGTAAAGAAAGAACAAAGCAAGAATGGGAAAAACTCATATACGATGCAGGTTTTAGCAGCTACAAAATTACCCCCATTTCTGGCTTCAAGTCACTCATTGAAGTTTATCCTTAG

Protein sequence

>MS.gene39534.t1

MAFTTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASSLKLHPSKVNILHRFLRLLTHNGFFAKTIVKGKEGEEEEIAYSLTPPSKLLISGKPTCLSSIVKGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQDAMASDSRMFKLVLQENKHVFEGLESLVDVGGGTGGVTKLINEIFPHLKCTVFDQPQVVGNLTGNENLNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLNILKNSKEAISHKGKDGKVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFKSLIEVYP