AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052008

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052008.t1 MTR_2g099030 99.206 378 3 0 1 378 30 407 0.0 783
MS.gene052008.t1 MTR_2g087780 46.825 378 189 7 1 374 23 392 2.30e-110 328
MS.gene052008.t1 MTR_7g490350 49.112 338 161 5 37 372 36 364 8.30e-106 316
MS.gene052008.t1 MTR_7g090300 48.598 321 155 4 53 372 53 364 1.70e-103 310
MS.gene052008.t1 MTR_4g050190 43.351 376 204 5 1 374 20 388 1.35e-101 306
MS.gene052008.t1 MTR_7g490330 48.036 331 124 4 43 372 37 320 3.76e-101 302
MS.gene052008.t1 MTR_5g091980 45.171 321 125 5 53 372 48 318 1.97e-84 259
MS.gene052008.t1 MTR_7g090320 38.056 360 202 6 21 367 26 377 4.27e-83 258
MS.gene052008.t1 MTR_7g090310 38.922 334 182 7 47 367 52 376 8.58e-82 255
MS.gene052008.t1 MTR_4g112670 33.234 334 194 4 41 373 27 332 2.87e-61 200
MS.gene052008.t1 MTR_4g112650 32.840 338 194 4 41 375 26 333 8.06e-59 194
MS.gene052008.t1 MTR_4g112630 33.433 335 179 5 41 371 26 320 5.00e-58 192
MS.gene052008.t1 MTR_2g058450 33.234 337 193 6 41 375 28 334 1.08e-54 183
MS.gene052008.t1 MTR_4g112620 28.916 332 169 5 41 371 30 295 2.13e-42 150
MS.gene052008.t1 MTR_8g074270 32.595 316 166 9 33 347 60 329 1.15e-41 150
MS.gene052008.t1 MTR_8g074170 33.948 271 149 6 33 303 60 300 1.18e-40 147
MS.gene052008.t1 MTR_8g074210 33.333 258 144 6 48 305 73 302 1.27e-38 142
MS.gene052008.t1 MTR_2g042370 29.329 283 189 6 95 376 84 356 4.85e-36 135
MS.gene052008.t1 MTR_2g058410 32.031 256 147 4 122 375 1 231 5.82e-34 126
MS.gene052008.t1 MTR_2g042430 26.899 316 209 6 64 378 75 369 3.17e-33 127
MS.gene052008.t1 MTR_2g042420 27.445 317 202 7 64 376 56 348 2.12e-31 122
MS.gene052008.t1 MTR_0003s0530 29.268 205 121 3 165 368 1 182 3.62e-25 101
MS.gene052008.t1 MTR_1g075865 41.905 105 61 0 170 274 7 111 1.35e-24 98.2
MS.gene052008.t1 MTR_8g074320 32.544 169 104 6 78 242 58 220 9.76e-20 87.4
MS.gene052008.t1 MTR_4g112840 38.776 98 60 0 198 295 63 160 1.18e-18 82.8
MS.gene052008.t1 MTR_2g058430 35.789 95 58 1 115 206 54 148 1.25e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052008.t1 AT3G59530 77.513 378 74 1 1 378 32 398 0.0 632
MS.gene052008.t1 AT3G59530 77.513 378 74 1 1 378 32 398 0.0 632
MS.gene052008.t1 AT3G59530 77.513 378 74 1 1 378 32 398 0.0 632
MS.gene052008.t1 AT5G22020 45.333 375 197 6 1 374 25 392 4.32e-107 320
MS.gene052008.t1 AT5G22020 45.333 375 197 6 1 374 25 392 4.32e-107 320
MS.gene052008.t1 AT5G22020 45.333 375 197 6 1 374 25 392 4.32e-107 320
MS.gene052008.t1 AT3G57030 49.371 318 149 5 53 367 56 364 6.40e-105 314
MS.gene052008.t1 AT1G08470 41.995 381 209 7 1 378 19 390 8.20e-101 304
MS.gene052008.t1 AT3G57020 41.250 320 174 5 51 367 51 359 3.66e-81 253
MS.gene052008.t1 AT3G57010 41.796 323 168 7 51 367 52 360 9.40e-78 244
MS.gene052008.t1 AT2G41300 41.667 312 167 5 59 367 84 383 2.32e-76 241
MS.gene052008.t1 AT3G57020 39.375 320 166 5 51 367 51 345 1.70e-75 238
MS.gene052008.t1 AT2G41290 39.939 328 176 8 51 367 49 366 2.88e-71 228
MS.gene052008.t1 AT1G74020 32.915 319 171 9 53 367 38 317 1.33e-49 170
MS.gene052008.t1 AT1G74010 33.129 326 178 9 44 367 28 315 1.47e-46 162
MS.gene052008.t1 AT1G74000 33.438 320 168 11 53 367 40 319 1.09e-45 160
MS.gene052008.t1 AT3G51440 30.746 335 183 9 54 378 70 365 4.85e-33 127
MS.gene052008.t1 AT3G51430 28.657 335 190 12 54 378 70 365 1.37e-31 123
MS.gene052008.t1 AT3G51430 28.060 335 190 12 54 378 70 363 3.80e-31 122
MS.gene052008.t1 AT3G51420 26.727 333 190 12 54 375 70 359 2.41e-30 119
MS.gene052008.t1 AT3G51450 29.080 337 186 12 54 378 70 365 2.52e-30 119

Find 95 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTCGAAAATGAAGTATT+TGG 0.199215 2.2:-3847984 MS.gene052008:CDS
TTCTTCATGACTTGTTTATA+TGG 0.232247 2.2:+3848053 None:intergenic
CATTCCACTGTTCCATCTTT+AGG 0.248214 2.2:+3846541 None:intergenic
GCCAACAAGCTCATTCTTAC+TGG 0.269587 2.2:+3846376 None:intergenic
TGCATATTATGGTCTTCTTA+TGG 0.279511 2.2:-3847394 MS.gene052008:CDS
TTCTCATGTTTCCATTGCTT+TGG 0.287013 2.2:+3847480 None:intergenic
CAAGACAACATGAGTTGTTT+TGG 0.309300 2.2:+3846933 None:intergenic
TACATAGCAGATGCATATTA+TGG 0.318723 2.2:-3847405 MS.gene052008:CDS
AATTGGTGGTTTCGGTAAAA+AGG 0.329225 2.2:+3846857 None:intergenic
TCATTCATTCTTACGTTGTC+TGG 0.331202 2.2:+3846496 None:intergenic
AAACCTGCAATTGGTGGTTT+CGG 0.351201 2.2:+3846849 None:intergenic
ATAAACAAGTCATGAAGAAT+TGG 0.371511 2.2:-3848049 MS.gene052008:CDS
AAGAGGAGAAAGGAAAGCTT+TGG 0.372961 2.2:-3846237 MS.gene052008:CDS
TTCGAGATTTGCACTCCATT+AGG 0.378181 2.2:+3846895 None:intergenic
AACGTAAGAATGAATGAAAA+AGG 0.383654 2.2:-3846490 MS.gene052008:CDS
CTTCTTATGGTTGGACCAAA+TGG 0.385238 2.2:-3847381 MS.gene052008:CDS
AACACGACCATCGGCTAAAC+CGG 0.389147 2.2:+3847917 None:intergenic
AAGATCATTTGCAAAGAGAA+TGG 0.401336 2.2:+3847317 None:intergenic
TGGTAGGAGGATCATATCTA+AGG 0.402934 2.2:+3846953 None:intergenic
CCTAGTGGACCTAAATGAAA+AGG 0.405868 2.2:+3848113 None:intergenic
ATGACAGGCTAATGAAACTT+TGG 0.408963 2.2:-3846573 MS.gene052008:intron
TATTATGGTCTTCTTATGGT+TGG 0.409388 2.2:-3847390 MS.gene052008:CDS
TTACCGAAACCACCAATTGC+AGG 0.427515 2.2:-3846852 MS.gene052008:intron
ATCTTGATATTCACAAGAAC+GGG 0.427957 2.2:-3847299 MS.gene052008:CDS
CTAATGAAACTTTGGATAGA+AGG 0.434236 2.2:-3846565 MS.gene052008:CDS
CCATCGGCTAAACCGGTGTA+AGG 0.444345 2.2:+3847924 None:intergenic
GCAATAGATTGTTGTCGAAC+TGG 0.446574 2.2:-3846454 MS.gene052008:CDS
CTTACGTTGTCTGGAAATCC+TGG 0.449914 2.2:+3846505 None:intergenic
TGGTCGTGTTGTCCGATGGA+TGG 0.450319 2.2:-3847904 MS.gene052008:CDS
GGTGTAGCCAGTCCTCCATT+TGG 0.451029 2.2:+3847366 None:intergenic
GCCAGTAAGAATGAGCTTGT+TGG 0.451834 2.2:-3846377 MS.gene052008:CDS
AGATCATTTGCAAAGAGAAT+GGG 0.452460 2.2:+3847318 None:intergenic
GGTCGTGTTGTCCGATGGAT+GGG 0.455520 2.2:-3847903 MS.gene052008:CDS
CAAAACAACTCATGTTGTCT+TGG 0.456064 2.2:-3846932 MS.gene052008:CDS
TTAAGATTTGACAAAGAGAG+TGG 0.459619 2.2:-3847435 MS.gene052008:CDS
CCTTCAACATGGGTTGCCAA+TGG 0.466610 2.2:+3847345 None:intergenic
GCTGGTTAGTGAAGTTAAAG+AGG 0.470251 2.2:-3846254 MS.gene052008:CDS
TTGCAGTTGTCACATCCAAT+TGG 0.472198 2.2:-3847857 MS.gene052008:intron
GAATGCGTAGCTGACCTTCC+AGG 0.474792 2.2:-3846523 MS.gene052008:CDS
GAGCTTGTTGGCTAAAGCTA+TGG 0.475927 2.2:-3846365 MS.gene052008:CDS
ATTGCACTTCTTGATGATAA+CGG 0.476011 2.2:-3846322 MS.gene052008:CDS
ATGAGCAGACTCGGACTTCA+CGG 0.478710 2.2:-3848014 MS.gene052008:CDS
GAGAAAGGAAAGCTTTGGAT+TGG 0.479363 2.2:-3846232 MS.gene052008:CDS
TTTATATTGTTGGAAGGAGA+AGG 0.479834 2.2:-3846988 MS.gene052008:CDS
GTCAGTTGAGTTCGATAACA+TGG 0.480155 2.2:-3847955 MS.gene052008:CDS
CTTTGTCAAATCTTAAACCA+AGG 0.491441 2.2:+3847442 None:intergenic
AGCTTGTTGGCTAAAGCTAT+GGG 0.498617 2.2:-3846364 MS.gene052008:CDS
CGAAAATGAAGTATTTGGAC+CGG 0.503096 2.2:-3847979 MS.gene052008:CDS
AAAACATGTGGGCGTCCCCT+TGG 0.510216 2.2:-3847459 MS.gene052008:CDS
AGTCTGCTCATGTTGTCCTT+AGG 0.510827 2.2:+3848026 None:intergenic
TGGATAGAAGGTCCTAAAGA+TGG 0.515483 2.2:-3846553 MS.gene052008:CDS
GTCGTGTTGTCCGATGGATG+GGG 0.520866 2.2:-3847902 MS.gene052008:CDS
AGTGAAGTTAAAGAGGAGAA+AGG 0.523089 2.2:-3846247 MS.gene052008:CDS
CCATTGGCAACCCATGTTGA+AGG 0.526859 2.2:-3847345 MS.gene052008:CDS
TGGAGGACTGGCTACACCAT+TGG 0.527000 2.2:-3847361 MS.gene052008:CDS
ACAACTCATGTTGTCTTGGA+TGG 0.528253 2.2:-3846928 MS.gene052008:CDS
CTAGTGGACCTAAATGAAAA+GGG 0.529354 2.2:+3848114 None:intergenic
TTGTTACTCAAGACTTCTTG+AGG 0.540862 2.2:+3846430 None:intergenic
TTTGTCAAATCTTAAACCAA+GGG 0.542290 2.2:+3847443 None:intergenic
TTGTTGGAAGGAGAAGGAAC+AGG 0.544523 2.2:-3846982 MS.gene052008:CDS
CCTTACACCGGTTTAGCCGA+TGG 0.545860 2.2:-3847924 MS.gene052008:CDS
GGTGCAACGTTGTGTTTCAC+AGG 0.546534 2.2:+3848074 None:intergenic
CCGATGGTCGTGTTGTCCGA+TGG 0.555843 2.2:-3847908 MS.gene052008:CDS
GAGTTCGATAACATGGGCCG+TGG 0.562804 2.2:-3847948 MS.gene052008:CDS
AAGTCTTGAGTAACAATCCA+TGG 0.565030 2.2:-3846423 MS.gene052008:CDS
GGTTGGACCAAATGGAGGAC+TGG 0.566119 2.2:-3847373 MS.gene052008:CDS
AAGGACAACATGAGCAGACT+CGG 0.574652 2.2:-3848023 MS.gene052008:CDS
GATCTTGATATTCACAAGAA+CGG 0.577423 2.2:-3847300 MS.gene052008:CDS
GACTGAGAAGATTTGCATGA+GGG 0.580450 2.2:-3847520 MS.gene052008:CDS
ATGGGCCGTGGCCCTTACAC+CGG 0.582691 2.2:-3847936 MS.gene052008:CDS
ACTCTACAACTCCAAAGCAA+TGG 0.585049 2.2:-3847491 MS.gene052008:CDS
CCATCGGACAACACGACCAT+CGG 0.587160 2.2:+3847908 None:intergenic
CTAAATTCATGTTCTCCTAG+TGG 0.592914 2.2:+3848098 None:intergenic
CTTATGGTTGGACCAAATGG+AGG 0.594581 2.2:-3847378 MS.gene052008:CDS
AGAAGGCAAAGTGATGAAGC+TGG 0.599296 2.2:-3846272 MS.gene052008:CDS
TTATCGAACTCAACTGACTC+CGG 0.603270 2.2:+3847960 None:intergenic
AGTCATGAAGAATTGGCCTA+AGG 0.608763 2.2:-3848042 MS.gene052008:CDS
GGACTGAGAAGATTTGCATG+AGG 0.612890 2.2:-3847521 MS.gene052008:CDS
CTAAACCGGTGTAAGGGCCA+CGG 0.618440 2.2:+3847931 None:intergenic
TGGAAACATGAGAAAACATG+TGG 0.620861 2.2:-3847471 MS.gene052008:CDS
TGAGTAACAATCCATGGTTG+AGG 0.621036 2.2:-3846417 MS.gene052008:CDS
ACTGAGAAGATTTGCATGAG+GGG 0.622020 2.2:-3847519 MS.gene052008:CDS
GCTTGTGTCAGTGAAGAAGA+TGG 0.622137 2.2:+3847275 None:intergenic
AGCTCATTCTTACTGGCAAG+CGG 0.627455 2.2:+3846383 None:intergenic
GAAGTTCTTGAAGATCGAGA+AGG 0.628335 2.2:-3846289 MS.gene052008:CDS
CATCGGCTAAACCGGTGTAA+GGG 0.633079 2.2:+3847925 None:intergenic
CGTTGTCTGGAAATCCTGGA+AGG 0.633711 2.2:+3846509 None:intergenic
TCGTGTTGTCCGATGGATGG+GGG 0.655214 2.2:-3847901 MS.gene052008:CDS
TTGTCAAATCTTAAACCAAG+GGG 0.656805 2.2:+3847444 None:intergenic
AAGTAAATACTCCTCAACCA+TGG 0.660467 2.2:+3846406 None:intergenic
TCAGTTGAGTTCGATAACAT+GGG 0.661205 2.2:-3847954 MS.gene052008:CDS
AGGTCCTAAAGATGGAACAG+TGG 0.678364 2.2:-3846545 MS.gene052008:CDS
TGGAAGGAGAAGGAACAGGT+AGG 0.689818 2.2:-3846978 MS.gene052008:CDS
GGAAACATGAGAAAACATGT+GGG 0.709710 2.2:-3847470 MS.gene052008:CDS
GCTTGTTGGCTAAAGCTATG+GGG 0.726642 2.2:-3846363 MS.gene052008:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTAATATAAAATA+CGG + chr2.2:3847161-3847180 None:intergenic 0.0%
!! AAAAAATCTACAATAAAATT+TGG - chr2.2:3847174-3847193 MS.gene052008:intron 10.0%
!!! ACATAATATTTTAGACTTAA+TGG + chr2.2:3846715-3846734 None:intergenic 15.0%
!!! TAATGAATTAGTTATTTTGT+TGG + chr2.2:3847706-3847725 None:intergenic 15.0%
!! AATCTACAATAAAATTTGGA+CGG - chr2.2:3847178-3847197 MS.gene052008:intron 20.0%
!! CTTAGCTGAAATAAATAAAT+AGG + chr2.2:3847262-3847281 None:intergenic 20.0%
!! TCATTAAATTCTTTAATGAC+AGG - chr2.2:3847720-3847739 MS.gene052008:intron 20.0%
!!! TGAAACTTGGTATTTTTAAA+AGG - chr2.2:3846766-3846785 MS.gene052008:intron 20.0%
! AAACAATAACACTCATCATA+AGG + chr2.2:3847286-3847305 None:intergenic 25.0%
! AAATAAATAAATAGGATCCC+TGG + chr2.2:3847254-3847273 None:intergenic 25.0%
! AACGTAAGAATGAATGAAAA+AGG - chr2.2:3847818-3847837 MS.gene052008:intron 25.0%
! ATAAACAAGTCATGAAGAAT+TGG - chr2.2:3846259-3846278 MS.gene052008:CDS 25.0%
! GAGAAAATGCAAAATAAGTA+CGG + chr2.2:3847517-3847536 None:intergenic 25.0%
! TGTAATACTTGAGGTTAAAT+GGG - chr2.2:3847544-3847563 MS.gene052008:intron 25.0%
! TTGTAATACTTGAGGTTAAA+TGG - chr2.2:3847543-3847562 MS.gene052008:intron 25.0%
! TTTGTCAAATCTTAAACCAA+GGG + chr2.2:3846868-3846887 None:intergenic 25.0%
!! GAATTCGAAAATGAAGTATT+TGG - chr2.2:3846324-3846343 MS.gene052008:CDS 25.0%
!! TACATTGTGATCTTTTATCT+TGG - chr2.2:3847585-3847604 MS.gene052008:intron 25.0%
!!! CACTTTTTTATATTGTTGGA+AGG - chr2.2:3847314-3847333 MS.gene052008:CDS 25.0%
!!! CGCTAAGGTTTTTTAAAAAA+TGG + chr2.2:3847620-3847639 None:intergenic 25.0%
!!! TGCACACTTTTTTATATTGT+TGG - chr2.2:3847310-3847329 MS.gene052008:CDS 25.0%
!!! TTCTTCATGACTTGTTTATA+TGG + chr2.2:3846258-3846277 None:intergenic 25.0%
AAGATCATTTGCAAAGAGAA+TGG + chr2.2:3846994-3847013 None:intergenic 30.0%
AGATCATTTGCAAAGAGAAT+GGG + chr2.2:3846993-3847012 None:intergenic 30.0%
ATCATTTGACCTCAATTCAA+CGG + chr2.2:3847124-3847143 None:intergenic 30.0%
ATCTTGATATTCACAAGAAC+GGG - chr2.2:3847009-3847028 MS.gene052008:intron 30.0%
CTAATGAAACTTTGGATAGA+AGG - chr2.2:3847743-3847762 MS.gene052008:intron 30.0%
CTTTGTCAAATCTTAAACCA+AGG + chr2.2:3846869-3846888 None:intergenic 30.0%
GATCTTGATATTCACAAGAA+CGG - chr2.2:3847008-3847027 MS.gene052008:intron 30.0%
TACATAGCAGATGCATATTA+TGG - chr2.2:3846903-3846922 MS.gene052008:CDS 30.0%
TATTATGGTCTTCTTATGGT+TGG - chr2.2:3846918-3846937 MS.gene052008:CDS 30.0%
TGCATATTATGGTCTTCTTA+TGG - chr2.2:3846914-3846933 MS.gene052008:CDS 30.0%
TTAAGATTTGACAAAGAGAG+TGG - chr2.2:3846873-3846892 MS.gene052008:CDS 30.0%
TTGTCAAATCTTAAACCAAG+GGG + chr2.2:3846867-3846886 None:intergenic 30.0%
TTTATATTGTTGGAAGGAGA+AGG - chr2.2:3847320-3847339 MS.gene052008:CDS 30.0%
! ATTGCACTTCTTGATGATAA+CGG - chr2.2:3847986-3848005 MS.gene052008:CDS 30.0%
! GAATGAAAAAGGACAGTTTT+GGG - chr2.2:3847829-3847848 MS.gene052008:intron 30.0%
! TGAATGAAAAAGGACAGTTT+TGG - chr2.2:3847828-3847847 MS.gene052008:intron 30.0%
!! GTTATTTTGTTGGACGAATT+TGG + chr2.2:3847696-3847715 None:intergenic 30.0%
AAGTAAATACTCCTCAACCA+TGG + chr2.2:3847905-3847924 None:intergenic 35.0%
AAGTCTTGAGTAACAATCCA+TGG - chr2.2:3847885-3847904 MS.gene052008:CDS 35.0%
AGTGAAGTTAAAGAGGAGAA+AGG - chr2.2:3848061-3848080 MS.gene052008:CDS 35.0%
AGTTAAAAACCTGCAATTGG+TGG + chr2.2:3847468-3847487 None:intergenic 35.0%
ATAAATAGGATCCCTGGATT+CGG + chr2.2:3847248-3847267 None:intergenic 35.0%
ATGACAGGCTAATGAAACTT+TGG - chr2.2:3847735-3847754 MS.gene052008:intron 35.0%
ATTGCAATGTGTCTCTCTAT+AGG + chr2.2:3846586-3846605 None:intergenic 35.0%
CAAAACAACTCATGTTGTCT+TGG - chr2.2:3847376-3847395 MS.gene052008:CDS 35.0%
CATTAGCATGAATGCACATA+TGG + chr2.2:3846748-3846767 None:intergenic 35.0%
CTAAATTCATGTTCTCCTAG+TGG + chr2.2:3846213-3846232 None:intergenic 35.0%
GCATTCATGCTAATGAAACT+TGG - chr2.2:3846753-3846772 MS.gene052008:intron 35.0%
GGAAACATGAGAAAACATGT+GGG - chr2.2:3846838-3846857 MS.gene052008:intron 35.0%
TCAGTTGAGTTCGATAACAT+GGG - chr2.2:3846354-3846373 MS.gene052008:CDS 35.0%
TCATTCATTCTTACGTTGTC+TGG + chr2.2:3847815-3847834 None:intergenic 35.0%
TCTGCAACTTGTAATACTTG+AGG - chr2.2:3847535-3847554 MS.gene052008:intron 35.0%
TGCAGTTAAAAACCTGCAAT+TGG + chr2.2:3847471-3847490 None:intergenic 35.0%
TGGAAACATGAGAAAACATG+TGG - chr2.2:3846837-3846856 MS.gene052008:intron 35.0%
TTCTCATGTTTCCATTGCTT+TGG + chr2.2:3846831-3846850 None:intergenic 35.0%
TTGTTACTCAAGACTTCTTG+AGG + chr2.2:3847881-3847900 None:intergenic 35.0%
! AATTGGTGGTTTCGGTAAAA+AGG + chr2.2:3847454-3847473 None:intergenic 35.0%
! CAAGACAACATGAGTTGTTT+TGG + chr2.2:3847378-3847397 None:intergenic 35.0%
! CGAAAATGAAGTATTTGGAC+CGG - chr2.2:3846329-3846348 MS.gene052008:CDS 35.0%
! CTAGTGGACCTAAATGAAAA+GGG + chr2.2:3846197-3846216 None:intergenic 35.0%
! TCTTGATGATACTCACCAAT+TGG + chr2.2:3846469-3846488 None:intergenic 35.0%
!! AATGGGTTTTCCTTCAACAT+GGG + chr2.2:3846976-3846995 None:intergenic 35.0%
!!! ACAACATGAGTTGTTTTGGT+AGG + chr2.2:3847374-3847393 None:intergenic 35.0%
!!! GATGGTCTTGTTTTTCCTAA+TGG - chr2.2:3847398-3847417 MS.gene052008:CDS 35.0%
AAACCTTAGCGACACGAAAT+TGG - chr2.2:3847629-3847648 MS.gene052008:intron 40.0%
AAGAGGAGAAAGGAAAGCTT+TGG - chr2.2:3848071-3848090 MS.gene052008:CDS 40.0%
ACAACTCATGTTGTCTTGGA+TGG - chr2.2:3847380-3847399 MS.gene052008:CDS 40.0%
ACTCTACAACTCCAAAGCAA+TGG - chr2.2:3846817-3846836 MS.gene052008:intron 40.0%
ACTGAGAAGATTTGCATGAG+GGG - chr2.2:3846789-3846808 MS.gene052008:intron 40.0%
CATTCCACTGTTCCATCTTT+AGG + chr2.2:3847770-3847789 None:intergenic 40.0%
CTTCTTATGGTTGGACCAAA+TGG - chr2.2:3846927-3846946 MS.gene052008:CDS 40.0%
GAAGTTCTTGAAGATCGAGA+AGG - chr2.2:3848019-3848038 MS.gene052008:CDS 40.0%
GACTGAGAAGATTTGCATGA+GGG - chr2.2:3846788-3846807 MS.gene052008:intron 40.0%
GCAATAGATTGTTGTCGAAC+TGG - chr2.2:3847854-3847873 MS.gene052008:intron 40.0%
GCTGCAGTATGATGTTGAAA+CGG + chr2.2:3847663-3847682 None:intergenic 40.0%
GCTGGTTAGTGAAGTTAAAG+AGG - chr2.2:3848054-3848073 MS.gene052008:CDS 40.0%
GTCAGTTGAGTTCGATAACA+TGG - chr2.2:3846353-3846372 MS.gene052008:CDS 40.0%
TAAATAGGATCCCTGGATTC+GGG + chr2.2:3847247-3847266 None:intergenic 40.0%
TGAGTAACAATCCATGGTTG+AGG - chr2.2:3847891-3847910 MS.gene052008:CDS 40.0%
TGGATAGAAGGTCCTAAAGA+TGG - chr2.2:3847755-3847774 MS.gene052008:intron 40.0%
TGGTAGGAGGATCATATCTA+AGG + chr2.2:3847358-3847377 None:intergenic 40.0%
TTCGAGATTTGCACTCCATT+AGG + chr2.2:3847416-3847435 None:intergenic 40.0%
TTGCAGTTGTCACATCCAAT+TGG - chr2.2:3846451-3846470 MS.gene052008:CDS 40.0%
! AAACCTGCAATTGGTGGTTT+CGG + chr2.2:3847462-3847481 None:intergenic 40.0%
! AGTCATGAAGAATTGGCCTA+AGG - chr2.2:3846266-3846285 MS.gene052008:CDS 40.0%
! CCTAGTGGACCTAAATGAAA+AGG + chr2.2:3846198-3846217 None:intergenic 40.0%
! CCTTTTCATTTAGGTCCACT+AGG - chr2.2:3846195-3846214 MS.gene052008:CDS 40.0%
! TTATCGAACTCAACTGACTC+CGG + chr2.2:3846351-3846370 None:intergenic 40.0%
!! AGCTTGTTGGCTAAAGCTAT+GGG - chr2.2:3847944-3847963 MS.gene052008:CDS 40.0%
!! GAATGGGTTTTCCTTCAACA+TGG + chr2.2:3846977-3846996 None:intergenic 40.0%
!! GAGAAAGGAAAGCTTTGGAT+TGG - chr2.2:3848076-3848095 MS.gene052008:CDS 40.0%
!! TGTTGATAGCCGTTGAATTG+AGG - chr2.2:3847112-3847131 MS.gene052008:intron 40.0%
!!! ACATGAGTTGTTTTGGTAGG+AGG + chr2.2:3847371-3847390 None:intergenic 40.0%
!!! TGAAAAAGGACAGTTTTGGG+TGG - chr2.2:3847832-3847851 MS.gene052008:intron 40.0%
AAATAGGATCCCTGGATTCG+GGG + chr2.2:3847246-3847265 None:intergenic 45.0%
AAGGACAACATGAGCAGACT+CGG - chr2.2:3846285-3846304 MS.gene052008:CDS 45.0%
AGAAGGCAAAGTGATGAAGC+TGG - chr2.2:3848036-3848055 MS.gene052008:CDS 45.0%
AGCTCATTCTTACTGGCAAG+CGG + chr2.2:3847928-3847947 None:intergenic 45.0%
AGGTCCTAAAGATGGAACAG+TGG - chr2.2:3847763-3847782 MS.gene052008:intron 45.0%
AGTCTGCTCATGTTGTCCTT+AGG + chr2.2:3846285-3846304 None:intergenic 45.0%
CTTACGTTGTCTGGAAATCC+TGG + chr2.2:3847806-3847825 None:intergenic 45.0%
CTTATGGTTGGACCAAATGG+AGG - chr2.2:3846930-3846949 MS.gene052008:CDS 45.0%
GCCAACAAGCTCATTCTTAC+TGG + chr2.2:3847935-3847954 None:intergenic 45.0%
GCCAGTAAGAATGAGCTTGT+TGG - chr2.2:3847931-3847950 MS.gene052008:CDS 45.0%
GCTTGTGTCAGTGAAGAAGA+TGG + chr2.2:3847036-3847055 None:intergenic 45.0%
GGACGGTCATCTCAATTCAA+CGG - chr2.2:3847195-3847214 MS.gene052008:intron 45.0%
GGACTGAGAAGATTTGCATG+AGG - chr2.2:3846787-3846806 MS.gene052008:intron 45.0%
GGCTATCAACAATCTGACTC+TGG + chr2.2:3847103-3847122 None:intergenic 45.0%
GGTTTCGGTAAAAAGGAGGA+AGG + chr2.2:3847447-3847466 None:intergenic 45.0%
TGGTGGTTTCGGTAAAAAGG+AGG + chr2.2:3847451-3847470 None:intergenic 45.0%
TTACCGAAACCACCAATTGC+AGG - chr2.2:3847456-3847475 MS.gene052008:CDS 45.0%
TTGTTGGAAGGAGAAGGAAC+AGG - chr2.2:3847326-3847345 MS.gene052008:CDS 45.0%
! AGTCCAATTTCGTGTCGCTA+AGG + chr2.2:3847635-3847654 None:intergenic 45.0%
! GCTTGTTGGCTAAAGCTATG+GGG - chr2.2:3847945-3847964 MS.gene052008:CDS 45.0%
!! GAGCTTGTTGGCTAAAGCTA+TGG - chr2.2:3847943-3847962 MS.gene052008:CDS 45.0%
ATGAGCAGACTCGGACTTCA+CGG - chr2.2:3846294-3846313 MS.gene052008:CDS 50.0%
CAAGTTGTTCCCCCATCCAT+CGG + chr2.2:3846419-3846438 None:intergenic 50.0%
CCATTGGCAACCCATGTTGA+AGG - chr2.2:3846963-3846982 MS.gene052008:CDS 50.0%
CGTTGTCTGGAAATCCTGGA+AGG + chr2.2:3847802-3847821 None:intergenic 50.0%
GATGGGGGAACAACTTGGTT+GGG - chr2.2:3846422-3846441 MS.gene052008:CDS 50.0%
GGTGCAACGTTGTGTTTCAC+AGG + chr2.2:3846237-3846256 None:intergenic 50.0%
TGGAAGGAGAAGGAACAGGT+AGG - chr2.2:3847330-3847349 MS.gene052008:CDS 50.0%
! AACACGACCATCGGCTAAAC+CGG + chr2.2:3846394-3846413 None:intergenic 50.0%
! ATGCATTGATCCCCGAATCC+AGG - chr2.2:3847233-3847252 MS.gene052008:intron 50.0%
! CATCGGCTAAACCGGTGTAA+GGG + chr2.2:3846386-3846405 None:intergenic 50.0%
! CCTTCAACATGGGTTGCCAA+TGG + chr2.2:3846966-3846985 None:intergenic 50.0%
! TGCATTGATCCCCGAATCCA+GGG - chr2.2:3847234-3847253 MS.gene052008:intron 50.0%
AAAACATGTGGGCGTCCCCT+TGG - chr2.2:3846849-3846868 MS.gene052008:intron 55.0%
CCATCGGACAACACGACCAT+CGG + chr2.2:3846403-3846422 None:intergenic 55.0%
CGATGGATGGGGGAACAACT+TGG - chr2.2:3846417-3846436 MS.gene052008:CDS 55.0%
CTAAACCGGTGTAAGGGCCA+CGG + chr2.2:3846380-3846399 None:intergenic 55.0%
GAATGCGTAGCTGACCTTCC+AGG - chr2.2:3847785-3847804 MS.gene052008:intron 55.0%
GAGTTCGATAACATGGGCCG+TGG - chr2.2:3846360-3846379 MS.gene052008:CDS 55.0%
GGATGGGGGAACAACTTGGT+TGG - chr2.2:3846421-3846440 MS.gene052008:CDS 55.0%
GGTCGTGTTGTCCGATGGAT+GGG - chr2.2:3846405-3846424 MS.gene052008:CDS 55.0%
GGTGTAGCCAGTCCTCCATT+TGG + chr2.2:3846945-3846964 None:intergenic 55.0%
GGTTGGACCAAATGGAGGAC+TGG - chr2.2:3846935-3846954 MS.gene052008:CDS 55.0%
GTCGTGTTGTCCGATGGATG+GGG - chr2.2:3846406-3846425 MS.gene052008:CDS 55.0%
TCGTGTTGTCCGATGGATGG+GGG - chr2.2:3846407-3846426 MS.gene052008:CDS 55.0%
TGGTCGTGTTGTCCGATGGA+TGG - chr2.2:3846404-3846423 MS.gene052008:CDS 55.0%
! CCATCGGCTAAACCGGTGTA+AGG + chr2.2:3846387-3846406 None:intergenic 55.0%
!! CCTTACACCGGTTTAGCCGA+TGG - chr2.2:3846384-3846403 MS.gene052008:CDS 55.0%
!! TGGAGGACTGGCTACACCAT+TGG - chr2.2:3846947-3846966 MS.gene052008:CDS 55.0%
CCGATGGTCGTGTTGTCCGA+TGG - chr2.2:3846400-3846419 MS.gene052008:CDS 60.0%
ATGGGCCGTGGCCCTTACAC+CGG - chr2.2:3846372-3846391 MS.gene052008:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3846189 3848141 3846189 ID=MS.gene052008
chr2.2 mRNA 3846189 3848141 3846189 ID=MS.gene052008.t1;Parent=MS.gene052008
chr2.2 exon 3847858 3848141 3847858 ID=MS.gene052008.t1.exon1;Parent=MS.gene052008.t1
chr2.2 CDS 3847858 3848141 3847858 ID=cds.MS.gene052008.t1;Parent=MS.gene052008.t1
chr2.2 exon 3847258 3847542 3847258 ID=MS.gene052008.t1.exon2;Parent=MS.gene052008.t1
chr2.2 CDS 3847258 3847542 3847258 ID=cds.MS.gene052008.t1;Parent=MS.gene052008.t1
chr2.2 exon 3846853 3847020 3846853 ID=MS.gene052008.t1.exon3;Parent=MS.gene052008.t1
chr2.2 CDS 3846853 3847020 3846853 ID=cds.MS.gene052008.t1;Parent=MS.gene052008.t1
chr2.2 exon 3846189 3846588 3846189 ID=MS.gene052008.t1.exon4;Parent=MS.gene052008.t1
chr2.2 CDS 3846189 3846588 3846189 ID=cds.MS.gene052008.t1;Parent=MS.gene052008.t1
Gene Sequence

>MS.gene052008

ATGGACCCTTTTCATTTAGGTCCACTAGGAGAACATGAATTTAGACCTGTGAAACACAACGTTGCACCATATAAACAAGTCATGAAGAATTGGCCTAAGGACAACATGAGCAGACTCGGACTTCACGGAAAATTAGAATTCGAAAATGAAGTATTTGGACCGGAGTCAGTTGAGTTCGATAACATGGGCCGTGGCCCTTACACCGGTTTAGCCGATGGTCGTGTTGTCCGATGGATGGGGGAACAACTTGGTTGGGAAACTTTTGCAGTTGTCACATCCAATTGGTGAGTATCATCAAGAAATTTGTAATCTAATTTGATTAAATCAGCTTTACAATACAACTCAAAATATGACATATAATTTTCATTCAACTATTATGATTCATGACTACGTGCCTATAGAGAGACACATTGCAATTATAATTGTTGAATGAAGATCAGACAATTCACATTTTGTGTTCGAAATATGAGATGTCTAAAATATGAAATGAAGATCTTCACGCAATTGCGTGATTGTATACACTCCATTAAGTCTAAAATATTATGTTTTTATATGTCCATATGTGCATTCATGCTAATGAAACTTGGTATTTTTAAAAGGACTGAGAAGATTTGCATGAGGGGAAATGACTCTACAACTCCAAAGCAATGGAAACATGAGAAAACATGTGGGCGTCCCCTTGGTTTAAGATTTGACAAAGAGAGTGGAGATTTATACATAGCAGATGCATATTATGGTCTTCTTATGGTTGGACCAAATGGAGGACTGGCTACACCATTGGCAACCCATGTTGAAGGAAAACCCATTCTCTTTGCAAATGATCTTGATATTCACAAGAACGGGTCCATCTTCTTCACTGACACAAGCACACGATACAATCGAGTGTAATTTCTTCTATTTCTATCTATTCACCAGAGTCAGATTGTTGATAGCCGTTGAATTGAGGTCAAATGATATAGATTTAAAGTTCCGTATTTTATATTAAAAAAAATCTACAATAAAATTTGGACGGTCATCTCAATTCAACGGTTATCGATATGTTAAATGCATTGATCCCCGAATCCAGGGATCCTATTTATTTATTTCAGCTAAGTCCTTATGATGAGTGTTATTGTTTGCAGTGCACACTTTTTTATATTGTTGGAAGGAGAAGGAACAGGTAGGCTCCTTAGATATGATCCTCCTACCAAAACAACTCATGTTGTCTTGGATGGTCTTGTTTTTCCTAATGGAGTGCAAATCTCGAAAGATCAATCCTTCCTCCTTTTTACCGAAACCACCAATTGCAGGTTTTTAACTGCATTAATTATTTCTTTATCAACATACCGTACTTATTTTGCATTTTCTCTGCAACTTGTAATACTTGAGGTTAAATGGGATGCAACTCAATTGTAGTTACATTGTGATCTTTTATCTTGGTGCTGCAAACCATTTTTTAAAAAACCTTAGCGACACGAAATTGGACTCTCTACCGTTTCAACATCATACTGCAGCTATAATGAATCCAAATTCGTCCAACAAAATAACTAATTCATTAAATTCTTTAATGACAGGCTAATGAAACTTTGGATAGAAGGTCCTAAAGATGGAACAGTGGAATGCGTAGCTGACCTTCCAGGATTTCCAGACAACGTAAGAATGAATGAAAAAGGACAGTTTTGGGTGGCAATAGATTGTTGTCGAACTGGACCTCAAGAAGTCTTGAGTAACAATCCATGGTTGAGGAGTATTTACTTCCGCTTGCCAGTAAGAATGAGCTTGTTGGCTAAAGCTATGGGGATGAAAATGTACACAATGATTGCACTTCTTGATGATAACGGAAAGATTCTTGAAGTTCTTGAAGATCGAGAAGGCAAAGTGATGAAGCTGGTTAGTGAAGTTAAAGAGGAGAAAGGAAAGCTTTGGATTGGAACAGTAGCTCATAATCACATCTCCACCTTACCTTACCCTTAA
Protein sequence

>MS.gene052008.t1

MDPFHLGPLGEHEFRPVKHNVAPYKQVMKNWPKDNMSRLGLHGKLEFENEVFGPESVEFDNMGRGPYTGLADGRVVRWMGEQLGWETFAVVTSNWTEKICMRGNDSTTPKQWKHEKTCGRPLGLRFDKESGDLYIADAYYGLLMVGPNGGLATPLATHVEGKPILFANDLDIHKNGSIFFTDTSTRYNRVAHFFILLEGEGTGRLLRYDPPTKTTHVVLDGLVFPNGVQISKDQSFLLFTETTNCRLMKLWIEGPKDGTVECVADLPGFPDNVRMNEKGQFWVAIDCCRTGPQEVLSNNPWLRSIYFRLPVRMSLLAKAMGMKMYTMIALLDDNGKILEVLEDREGKVMKLVSEVKEEKGKLWIGTVAHNHISTLPYP