AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88768


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88768.t1 MTR_5g069990 86.000 100 2 1 1 100 1 88 3.47e-53 162
MS.gene88768.t1 MTR_3g462990 67.692 65 16 3 11 73 10 71 1.55e-20 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88768.t1 AT3G47070 50.000 64 29 2 1 64 1 61 5.38e-14 63.2

Find 28 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAAGAAGAAGGGTGGTTT+TGG 0.266622 5.4:-54971537 MS.gene88768:CDS
CTTCCTGTTGTTGGAACCAT+TGG 0.379541 5.4:+54971826 None:intergenic
GCAAAGACTCTTCCTGTTGT+TGG 0.385540 5.4:+54971817 None:intergenic
TCTTCAACCTTCTTGAGAAT+AGG 0.417605 5.4:+54971682 None:intergenic
TTGCCAATGGTTCCAACAAC+AGG 0.422646 5.4:-54971829 MS.gene88768:CDS
GGAAGAGTCTTTGCAGCTAC+AGG 0.461997 5.4:-54971808 MS.gene88768:CDS
TTCTATCACCATGTTGCCAA+TGG 0.469167 5.4:-54971842 MS.gene88768:CDS
AGAGGCACTACTAGCAGTAA+AGG 0.496778 5.4:-54971646 MS.gene88768:CDS
GGCAGTAGCAAGCAAGAGAA+AGG 0.509106 5.4:-54971763 MS.gene88768:CDS
GGTAGTTCCACAAAAGAAGA+AGG 0.520914 5.4:-54971547 MS.gene88768:CDS
AGAGGTACTACTAGTGGTAG+AGG 0.535016 5.4:-54971613 MS.gene88768:CDS
ACAGGTGCAAAGGGTACTAC+AGG 0.544200 5.4:-54971790 MS.gene88768:CDS
AACTGATCCTATTCTCAAGA+AGG 0.548104 5.4:-54971689 MS.gene88768:CDS
CTTTGCAGCTACAGGTGCAA+AGG 0.552868 5.4:-54971800 MS.gene88768:CDS
GCTAGTGGTGGCAAGAACTC+TGG 0.553210 5.4:-54971568 MS.gene88768:CDS
TTTGCAGCTACAGGTGCAAA+GGG 0.555050 5.4:-54971799 MS.gene88768:CDS
GCAAAGGGTACTACAGGTGG+TGG 0.567835 5.4:-54971784 MS.gene88768:CDS
AGAGGTACTACTAGTGGTAG+AGG 0.570979 5.4:-54971595 MS.gene88768:CDS
AGTAAAGGCACTACTAGTAG+AGG 0.599998 5.4:-54971631 MS.gene88768:CDS
GAAGAGAAGAATAATAGTAG+AGG 0.604921 5.4:-54971664 MS.gene88768:CDS
TGTTGGAACCATTGGCAACA+TGG 0.606092 5.4:+54971834 None:intergenic
AGTGGTAGAGGCACTGCTAG+TGG 0.617969 5.4:-54971583 MS.gene88768:CDS
GTAGTTCCACAAAAGAAGAA+GGG 0.642030 5.4:-54971546 MS.gene88768:CDS
GTTCCACAAAAGAAGAAGGG+TGG 0.647995 5.4:-54971543 MS.gene88768:CDS
ACTAGTAGAGGTACTACTAG+TGG 0.652621 5.4:-54971619 MS.gene88768:CDS
AGTGGTAGAGGTACTACTAG+TGG 0.660105 5.4:-54971601 MS.gene88768:CDS
GGTAGAGGCACTGCTAGTGG+TGG 0.662360 5.4:-54971580 MS.gene88768:CDS
GGTGCAAAGGGTACTACAGG+TGG 0.681783 5.4:-54971787 MS.gene88768:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAAGAGAAGAATAATAGTAG+AGG - chr5.4:54971708-54971727 MS.gene88768:CDS 30.0%
AACTGATCCTATTCTCAAGA+AGG - chr5.4:54971683-54971702 MS.gene88768:CDS 35.0%
GTAGTTCCACAAAAGAAGAA+GGG - chr5.4:54971826-54971845 MS.gene88768:CDS 35.0%
TCTTCAACCTTCTTGAGAAT+AGG + chr5.4:54971693-54971712 None:intergenic 35.0%
! CAAGCAAGAGAAAGGTTTTT+GGG - chr5.4:54971617-54971636 MS.gene88768:CDS 35.0%
!!! AAGAGAAAGGTTTTTGGGAT+TGG - chr5.4:54971622-54971641 MS.gene88768:CDS 35.0%
GGTAGTTCCACAAAAGAAGA+AGG - chr5.4:54971825-54971844 MS.gene88768:CDS 40.0%
TTCTATCACCATGTTGCCAA+TGG - chr5.4:54971530-54971549 MS.gene88768:CDS 40.0%
! AAACCACCCTTCTTCTTTTG+TGG + chr5.4:54971835-54971854 None:intergenic 40.0%
! CAAAAGAAGAAGGGTGGTTT+TGG - chr5.4:54971835-54971854 MS.gene88768:CDS 40.0%
! GCAAGCAAGAGAAAGGTTTT+TGG - chr5.4:54971616-54971635 MS.gene88768:CDS 40.0%
!! ACTAGTAGAGGTACTACTAG+TGG - chr5.4:54971753-54971772 MS.gene88768:CDS 40.0%
!! AGTAAAGGCACTACTAGTAG+AGG - chr5.4:54971741-54971760 MS.gene88768:CDS 40.0%
!!! GGTTTTTGGGATTGGATTGT+TGG - chr5.4:54971630-54971649 MS.gene88768:CDS 40.0%
!!! TTTTGGGATTGGATTGTTGG+TGG - chr5.4:54971633-54971652 MS.gene88768:CDS 40.0%
CTTCCTGTTGTTGGAACCAT+TGG + chr5.4:54971549-54971568 None:intergenic 45.0%
GCAAAGACTCTTCCTGTTGT+TGG + chr5.4:54971558-54971577 None:intergenic 45.0%
GTTCCACAAAAGAAGAAGGG+TGG - chr5.4:54971829-54971848 MS.gene88768:CDS 45.0%
TGTTGGAACCATTGGCAACA+TGG + chr5.4:54971541-54971560 None:intergenic 45.0%
TTGCCAATGGTTCCAACAAC+AGG - chr5.4:54971543-54971562 MS.gene88768:CDS 45.0%
TTTGCAGCTACAGGTGCAAA+GGG - chr5.4:54971573-54971592 MS.gene88768:CDS 45.0%
!! AGAGGCACTACTAGCAGTAA+AGG - chr5.4:54971726-54971745 MS.gene88768:CDS 45.0%
!! AGAGGTACTACTAGTGGTAG+AGG - chr5.4:54971759-54971778 MS.gene88768:CDS 45.0%
!! AGTGGTAGAGGTACTACTAG+TGG - chr5.4:54971771-54971790 MS.gene88768:CDS 45.0%
!!! AAGAAGAAGGGTGGTTTTGG+AGG - chr5.4:54971838-54971857 MS.gene88768:CDS 45.0%
CTTTGCAGCTACAGGTGCAA+AGG - chr5.4:54971572-54971591 MS.gene88768:CDS 50.0%
GGAAGAGTCTTTGCAGCTAC+AGG - chr5.4:54971564-54971583 MS.gene88768:CDS 50.0%
GGCAGTAGCAAGCAAGAGAA+AGG - chr5.4:54971609-54971628 MS.gene88768:CDS 50.0%
!! ACAGGTGCAAAGGGTACTAC+AGG - chr5.4:54971582-54971601 MS.gene88768:CDS 50.0%
GCTAGTGGTGGCAAGAACTC+TGG - chr5.4:54971804-54971823 MS.gene88768:CDS 55.0%
!! AGTGGTAGAGGCACTGCTAG+TGG - chr5.4:54971789-54971808 MS.gene88768:CDS 55.0%
!! GCAAAGGGTACTACAGGTGG+TGG - chr5.4:54971588-54971607 MS.gene88768:CDS 55.0%
!! GGTGCAAAGGGTACTACAGG+TGG - chr5.4:54971585-54971604 MS.gene88768:CDS 55.0%
!! GGTAGAGGCACTGCTAGTGG+TGG - chr5.4:54971792-54971811 MS.gene88768:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 54971525 54971869 54971525 ID=MS.gene88768
chr5.4 mRNA 54971525 54971869 54971525 ID=MS.gene88768.t1;Parent=MS.gene88768
chr5.4 exon 54971525 54971869 54971525 ID=MS.gene88768.t1.exon1;Parent=MS.gene88768.t1
chr5.4 CDS 54971525 54971869 54971525 ID=cds.MS.gene88768.t1;Parent=MS.gene88768.t1
Gene Sequence

>MS.gene88768

ATGGCTTCTATCACCATGTTGCCAATGGTTCCAACAACAGGAAGAGTCTTTGCAGCTACAGGTGCAAAGGGTACTACAGGTGGTGGCAGTAGCAAGCAAGAGAAAGGTTTTTGGGATTGGATTGTTGGTGGTTTAACAAAAGAAGATCAGTTCTATGAAACTGATCCTATTCTCAAGAAGGTTGAAGAGAAGAATAATAGTAGAGGCACTACTAGCAGTAAAGGCACTACTAGTAGAGGTACTACTAGTGGTAGAGGTACTACTAGTGGTAGAGGCACTGCTAGTGGTGGCAAGAACTCTGGTAGTTCCACAAAAGAAGAAGGGTGGTTTTGGAGGATTTTTTAA

Protein sequence

>MS.gene88768.t1

MASITMLPMVPTTGRVFAATGAKGTTGGGSSKQEKGFWDWIVGGLTKEDQFYETDPILKKVEEKNNSRGTTSSKGTTSRGTTSGRGTTSGRGTASGGKNSGSSTKEEGWFWRIF