AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43782


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43782.t1 MTR_5g069990 100.000 106 0 0 1 106 1 106 8.45e-72 209
MS.gene43782.t1 MTR_3g462990 56.250 96 26 5 1 94 1 82 1.39e-22 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43782.t1 AT3G47070 50.000 64 29 2 1 64 1 61 1.02e-13 62.0

Find 25 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAAGAAGAAGGGTGGTTT+TGG 0.266622 5.1:-54434466 MS.gene43782:CDS
CTTCCTGTTGTTGGAACCAT+TGG 0.379541 5.1:+54434721 None:intergenic
GCAAAGACTCTTCCTGTTGT+TGG 0.385540 5.1:+54434712 None:intergenic
TCTTCAACCTTCTTGAGAAT+AGG 0.417605 5.1:+54434577 None:intergenic
TTGCCAATGGTTCCAACAAC+AGG 0.422646 5.1:-54434724 MS.gene43782:CDS
GGAAGAGTCTTTGCAGCTAC+AGG 0.461997 5.1:-54434703 MS.gene43782:CDS
TTCTATCACCATGTTGCCAA+TGG 0.469167 5.1:-54434737 MS.gene43782:CDS
GGCAGTAGCAAGCAAGAGAA+AGG 0.509106 5.1:-54434658 MS.gene43782:CDS
AGAGGTACTACTAGTGGTAA+AGG 0.534650 5.1:-54434526 MS.gene43782:CDS
GGTAGTTCCACAAAAGAAGA+AGG 0.536969 5.1:-54434476 MS.gene43782:CDS
ACAGGTGCAAAGGGTACTAC+AGG 0.544200 5.1:-54434685 MS.gene43782:CDS
AACTGATCCTATTCTCAAGA+AGG 0.548104 5.1:-54434584 MS.gene43782:CDS
CTTTGCAGCTACAGGTGCAA+AGG 0.552868 5.1:-54434695 MS.gene43782:CDS
TTTGCAGCTACAGGTGCAAA+GGG 0.555050 5.1:-54434694 MS.gene43782:CDS
GCAAAGGGTACTACAGGTGG+TGG 0.567835 5.1:-54434679 MS.gene43782:CDS
GAAGAGAAGAATAATAGTAG+AGG 0.604921 5.1:-54434559 MS.gene43782:CDS
TGTTGGAACCATTGGCAACA+TGG 0.606092 5.1:+54434729 None:intergenic
AGTGGTAAAGGCACTACTAG+TGG 0.623495 5.1:-54434514 MS.gene43782:CDS
AGTAGAGGCACTACTAGTAG+AGG 0.624915 5.1:-54434544 MS.gene43782:CDS
GTTCCACAAAAGAAGAAGGG+TGG 0.647995 5.1:-54434472 MS.gene43782:CDS
GGTAAAGGCACTACTAGTGG+TGG 0.650081 5.1:-54434511 MS.gene43782:CDS
ACTAGTAGAGGTACTACTAG+TGG 0.652621 5.1:-54434532 MS.gene43782:CDS
GTAGTTCCACAAAAGAAGAA+GGG 0.659042 5.1:-54434475 MS.gene43782:CDS
GGTGCAAAGGGTACTACAGG+TGG 0.681783 5.1:-54434682 MS.gene43782:CDS
TAGTGGTGGCAAGAACTCTG+TGG 0.685628 5.1:-54434497 MS.gene43782:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGGAGGATTTTTTAACA+AGG - chr5.1:54434737-54434756 MS.gene43782:CDS 25.0%
GAAGAGAAGAATAATAGTAG+AGG - chr5.1:54434627-54434646 MS.gene43782:CDS 30.0%
AACTGATCCTATTCTCAAGA+AGG - chr5.1:54434602-54434621 MS.gene43782:CDS 35.0%
GTAGTTCCACAAAAGAAGAA+GGG - chr5.1:54434711-54434730 MS.gene43782:CDS 35.0%
TCTTCAACCTTCTTGAGAAT+AGG + chr5.1:54434612-54434631 None:intergenic 35.0%
! CAAGCAAGAGAAAGGTTTTT+GGG - chr5.1:54434536-54434555 MS.gene43782:CDS 35.0%
!!! AAGAGAAAGGTTTTTGGGAT+TGG - chr5.1:54434541-54434560 MS.gene43782:CDS 35.0%
GGTAGTTCCACAAAAGAAGA+AGG - chr5.1:54434710-54434729 MS.gene43782:CDS 40.0%
TTCTATCACCATGTTGCCAA+TGG - chr5.1:54434449-54434468 MS.gene43782:CDS 40.0%
! AAACCACCCTTCTTCTTTTG+TGG + chr5.1:54434720-54434739 None:intergenic 40.0%
! CAAAAGAAGAAGGGTGGTTT+TGG - chr5.1:54434720-54434739 MS.gene43782:CDS 40.0%
! GCAAGCAAGAGAAAGGTTTT+TGG - chr5.1:54434535-54434554 MS.gene43782:CDS 40.0%
!! ACTAGTAGAGGTACTACTAG+TGG - chr5.1:54434654-54434673 MS.gene43782:CDS 40.0%
!! AGAGGTACTACTAGTGGTAA+AGG - chr5.1:54434660-54434679 MS.gene43782:CDS 40.0%
!!! GGTTTTTGGGATTGGATTGT+TGG - chr5.1:54434549-54434568 MS.gene43782:CDS 40.0%
!!! TTTTGGGATTGGATTGTTGG+TGG - chr5.1:54434552-54434571 MS.gene43782:CDS 40.0%
CTTCCTGTTGTTGGAACCAT+TGG + chr5.1:54434468-54434487 None:intergenic 45.0%
GCAAAGACTCTTCCTGTTGT+TGG + chr5.1:54434477-54434496 None:intergenic 45.0%
GTTCCACAAAAGAAGAAGGG+TGG - chr5.1:54434714-54434733 MS.gene43782:CDS 45.0%
TGTTGGAACCATTGGCAACA+TGG + chr5.1:54434460-54434479 None:intergenic 45.0%
TTGCCAATGGTTCCAACAAC+AGG - chr5.1:54434462-54434481 MS.gene43782:CDS 45.0%
TTTGCAGCTACAGGTGCAAA+GGG - chr5.1:54434492-54434511 MS.gene43782:CDS 45.0%
!! AGTAGAGGCACTACTAGTAG+AGG - chr5.1:54434642-54434661 MS.gene43782:CDS 45.0%
!! AGTGGTAAAGGCACTACTAG+TGG - chr5.1:54434672-54434691 MS.gene43782:CDS 45.0%
!!! AAGAAGAAGGGTGGTTTTGG+AGG - chr5.1:54434723-54434742 MS.gene43782:CDS 45.0%
CTTTGCAGCTACAGGTGCAA+AGG - chr5.1:54434491-54434510 MS.gene43782:CDS 50.0%
GGAAGAGTCTTTGCAGCTAC+AGG - chr5.1:54434483-54434502 MS.gene43782:CDS 50.0%
GGCAGTAGCAAGCAAGAGAA+AGG - chr5.1:54434528-54434547 MS.gene43782:CDS 50.0%
TAGTGGTGGCAAGAACTCTG+TGG - chr5.1:54434689-54434708 MS.gene43782:CDS 50.0%
!! ACAGGTGCAAAGGGTACTAC+AGG - chr5.1:54434501-54434520 MS.gene43782:CDS 50.0%
!! GGTAAAGGCACTACTAGTGG+TGG - chr5.1:54434675-54434694 MS.gene43782:CDS 50.0%
!! GCAAAGGGTACTACAGGTGG+TGG - chr5.1:54434507-54434526 MS.gene43782:CDS 55.0%
!! GGTGCAAAGGGTACTACAGG+TGG - chr5.1:54434504-54434523 MS.gene43782:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 54434444 54434764 54434444 ID=MS.gene43782
chr5.1 mRNA 54434444 54434764 54434444 ID=MS.gene43782.t1;Parent=MS.gene43782
chr5.1 exon 54434444 54434764 54434444 ID=MS.gene43782.t1.exon1;Parent=MS.gene43782.t1
chr5.1 CDS 54434444 54434764 54434444 ID=cds.MS.gene43782.t1;Parent=MS.gene43782.t1
Gene Sequence

>MS.gene43782

ATGGCTTCTATCACCATGTTGCCAATGGTTCCAACAACAGGAAGAGTCTTTGCAGCTACAGGTGCAAAGGGTACTACAGGTGGTGGCAGTAGCAAGCAAGAGAAAGGTTTTTGGGATTGGATTGTTGGTGGTTTAACAAAAGAAGATCAGTTCTATGAAACTGATCCTATTCTCAAGAAGGTTGAAGAGAAGAATAATAGTAGAGGCACTACTAGTAGAGGTACTACTAGTGGTAAAGGCACTACTAGTGGTGGCAAGAACTCTGTGGTAGTTCCACAAAAGAAGAAGGGTGGTTTTGGAGGATTTTTTAACAAGGATTAA

Protein sequence

>MS.gene43782.t1

MASITMLPMVPTTGRVFAATGAKGTTGGGSSKQEKGFWDWIVGGLTKEDQFYETDPILKKVEEKNNSRGTTSRGTTSGKGTTSGGKNSVVVPQKKKGGFGGFFNKD