AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068064


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068064.t1 MTR_7g088210 98.319 119 2 0 1 119 134 252 1.27e-84 247
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068064.t1 AT2G41120 58.065 124 44 2 1 118 136 257 6.64e-46 149

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGATATTTCAGCAGTTT+TGG 0.240858 7.2:-20530830 MS.gene068064:CDS
AGAAAGATCCTACCAACTTA+TGG 0.295152 7.2:-20530516 MS.gene068064:CDS
AAAGGAGCTATGATGGAGTT+AGG 0.335188 7.2:-20530913 None:intergenic
AACTTAAGAAACGGTCCATT+AGG 0.336744 7.2:+20530856 None:intergenic
AAGGAGCTATGATGGAGTTA+GGG 0.411699 7.2:-20530912 None:intergenic
GAAAGATCCTACCAACTTAT+GGG 0.416370 7.2:-20530515 MS.gene068064:CDS
CTCCAAGGGCAAAGCTTCCC+AGG 0.433451 7.2:-20530391 MS.gene068064:CDS
TACATTGTGTTCTGCCCTCA+AGG 0.434776 7.2:-20530434 MS.gene068064:CDS
CTTTGCCCTTGGAGCTTCAG+TGG 0.439060 7.2:+20530400 None:intergenic
CGCGTATATGATCTAGAACT+TGG 0.442298 7.2:-20530473 MS.gene068064:CDS
ACTTAAGAAACGGTCCATTA+GGG 0.450454 7.2:+20530857 None:intergenic
CAGCTAACTCTATCTGTGTC+TGG 0.459791 7.2:+20530797 None:intergenic
AGAAAGATGGAGTTCCCTAA+TGG 0.471693 7.2:-20530871 MS.gene068064:CDS
AGCCTGGGAAGCTTTGCCCT+TGG 0.483988 7.2:+20530389 None:intergenic
TCTTTCTCAAACTTAAGAAA+CGG 0.509201 7.2:+20530847 None:intergenic
TGGCATCATTAGACCCTTGA+GGG 0.509241 7.2:+20530420 None:intergenic
TTCTGAGGTAGCATTAAGCC+TGG 0.518926 7.2:+20530373 None:intergenic
CAGCATATTCTCCCATAAGT+TGG 0.529568 7.2:+20530504 None:intergenic
ACTATGTAAATTGAGAAAGA+TGG 0.536572 7.2:-20530884 MS.gene068064:CDS
CTTATGGGAGAATATGCTGC+AGG 0.540524 7.2:-20530500 MS.gene068064:CDS
TGATGATGTTTGTGTTGCAA+TGG 0.550109 7.2:-20530546 MS.gene068064:CDS
TGATGCCACTGAAGCTCCAA+GGG 0.576853 7.2:-20530405 MS.gene068064:CDS
ACATTGTGTTCTGCCCTCAA+GGG 0.584678 7.2:-20530433 MS.gene068064:CDS
ATGATGCCACTGAAGCTCCA+AGG 0.592193 7.2:-20530406 MS.gene068064:CDS
ATATTCTCCCATAAGTTGGT+AGG 0.594558 7.2:+20530508 None:intergenic
AGGAGCTATGATGGAGTTAG+GGG 0.608325 7.2:-20530911 None:intergenic
AGCTATGATGGAGTTAGGGG+AGG 0.617037 7.2:-20530908 None:intergenic
GTGGCATCATTAGACCCTTG+AGG 0.624422 7.2:+20530419 None:intergenic
GCTATGATGGAGTTAGGGGA+GGG 0.630986 7.2:-20530907 MS.gene068064:CDS
ATATGCTACAAGATGTTCTG+AGG 0.636965 7.2:+20530358 None:intergenic
TCTGAGGTAGCATTAAGCCT+GGG 0.648996 7.2:+20530374 None:intergenic
TTATGGGAGAATATGCTGCA+GGG 0.653319 7.2:-20530499 MS.gene068064:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGATACATAACATTTCA+AGG + chr7.2:20530496-20530515 None:intergenic 20.0%
!! ATTCACAAATGTTATATGAT+CGG - chr7.2:20530638-20530657 MS.gene068064:intron 20.0%
!! GAATTAAATCTAATCTTGAA+TGG + chr7.2:20530622-20530641 None:intergenic 20.0%
! ACTATGTAAATTGAGAAAGA+TGG - chr7.2:20530366-20530385 MS.gene068064:CDS 25.0%
! TCTTTCTCAAACTTAAGAAA+CGG + chr7.2:20530406-20530425 None:intergenic 25.0%
!!! AAATGTTATATGATCGGTTT+TGG - chr7.2:20530644-20530663 MS.gene068064:intron 25.0%
!!! TACTGAGTTCAATTTTGATT+CGG + chr7.2:20530543-20530562 None:intergenic 25.0%
!!! TTTTTAACTTGAATTTGAGC+AGG - chr7.2:20530678-20530697 MS.gene068064:intron 25.0%
ATCTAATCTTGAATGGTAAC+AGG + chr7.2:20530615-20530634 None:intergenic 30.0%
GATACATAACATTTCAAGGT+TGG + chr7.2:20530492-20530511 None:intergenic 30.0%
! GAAAGATATTTCAGCAGTTT+TGG - chr7.2:20530420-20530439 MS.gene068064:CDS 30.0%
AACTTAAGAAACGGTCCATT+AGG + chr7.2:20530397-20530416 None:intergenic 35.0%
ACTTAAGAAACGGTCCATTA+GGG + chr7.2:20530396-20530415 None:intergenic 35.0%
AGAAAGATCCTACCAACTTA+TGG - chr7.2:20530734-20530753 MS.gene068064:intron 35.0%
ATATGCTACAAGATGTTCTG+AGG + chr7.2:20530895-20530914 None:intergenic 35.0%
GAAAGATCCTACCAACTTAT+GGG - chr7.2:20530735-20530754 MS.gene068064:intron 35.0%
! TGATGATGTTTGTGTTGCAA+TGG - chr7.2:20530704-20530723 MS.gene068064:intron 35.0%
!! ATATTCTCCCATAAGTTGGT+AGG + chr7.2:20530745-20530764 None:intergenic 35.0%
AGAAAGATGGAGTTCCCTAA+TGG - chr7.2:20530379-20530398 MS.gene068064:CDS 40.0%
CAGCATATTCTCCCATAAGT+TGG + chr7.2:20530749-20530768 None:intergenic 40.0%
TTATGGGAGAATATGCTGCA+GGG - chr7.2:20530751-20530770 MS.gene068064:intron 40.0%
! CGCGTATATGATCTAGAACT+TGG - chr7.2:20530777-20530796 MS.gene068064:intron 40.0%
ACATTGTGTTCTGCCCTCAA+GGG - chr7.2:20530817-20530836 MS.gene068064:CDS 45.0%
CAGCTAACTCTATCTGTGTC+TGG + chr7.2:20530456-20530475 None:intergenic 45.0%
CTTATGGGAGAATATGCTGC+AGG - chr7.2:20530750-20530769 MS.gene068064:intron 45.0%
TACATTGTGTTCTGCCCTCA+AGG - chr7.2:20530816-20530835 MS.gene068064:CDS 45.0%
TGGCATCATTAGACCCTTGA+GGG + chr7.2:20530833-20530852 None:intergenic 45.0%
! TCTGAGGTAGCATTAAGCCT+GGG + chr7.2:20530879-20530898 None:intergenic 45.0%
!! TTCTGAGGTAGCATTAAGCC+TGG + chr7.2:20530880-20530899 None:intergenic 45.0%
ATGATGCCACTGAAGCTCCA+AGG - chr7.2:20530844-20530863 MS.gene068064:CDS 50.0%
GTGGCATCATTAGACCCTTG+AGG + chr7.2:20530834-20530853 None:intergenic 50.0%
TGATGCCACTGAAGCTCCAA+GGG - chr7.2:20530845-20530864 MS.gene068064:CDS 50.0%
CTTTGCCCTTGGAGCTTCAG+TGG + chr7.2:20530853-20530872 None:intergenic 55.0%
AGCCTGGGAAGCTTTGCCCT+TGG + chr7.2:20530864-20530883 None:intergenic 60.0%
CTCCAAGGGCAAAGCTTCCC+AGG - chr7.2:20530859-20530878 MS.gene068064:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 20530346 20530926 20530346 ID=MS.gene068064
chr7.2 mRNA 20530346 20530926 20530346 ID=MS.gene068064.t1;Parent=MS.gene068064
chr7.2 exon 20530794 20530926 20530794 ID=MS.gene068064.t1.exon1;Parent=MS.gene068064.t1
chr7.2 CDS 20530794 20530926 20530794 ID=cds.MS.gene068064.t1;Parent=MS.gene068064.t1
chr7.2 exon 20530346 20530572 20530346 ID=MS.gene068064.t1.exon2;Parent=MS.gene068064.t1
chr7.2 CDS 20530346 20530572 20530346 ID=cds.MS.gene068064.t1;Parent=MS.gene068064.t1
Gene Sequence

>MS.gene068064

ATGATGGAGTTAGGGGAGGGACTATGTAAATTGAGAAAGATGGAGTTCCCTAATGGACCGTTTCTTAAGTTTGAGAAAGATATTTCAGCAGTTTTGGAATTTATCTACCAGACACAGATAGAGTTAGCTGCATGTGAGTAATTCCAACCTTGAAATGTTATGTATCATATGATACATGCAAGTTGTATCTTATTCCGAATCAAAATTGAACTCAGTAAATACAAGTCATTATGCATAGATATGTGTCATGAAGTTCATGTATCTTACCTGTTACCATTCAAGATTAGATTTAATTCACAAATGTTATATGATCGGTTTTGGAGAAATTACATTTTTTAACTTGAATTTGAGCAGGTAGTGATGATGTTTGTGTTGCAATGGATCAATCAGAAAGATCCTACCAACTTATGGGAGAATATGCTGCAGGGAAGCGCGTATATGATCTAGAACTTGGTCGTGATGCATCAGTGTACATTGTGTTCTGCCCTCAAGGGTCTAATGATGCCACTGAAGCTCCAAGGGCAAAGCTTCCCAGGCTTAATGCTACCTCAGAACATCTTGTAGCATATGAATACAAGTAA

Protein sequence

>MS.gene068064.t1

MMELGEGLCKLRKMEFPNGPFLKFEKDISAVLEFIYQTQIELAACSDDVCVAMDQSERSYQLMGEYAAGKRVYDLELGRDASVYIVFCPQGSNDATEAPRAKLPRLNATSEHLVAYEYK