AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22721


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22721.t1 MTR_7g117900 93.913 230 9 1 1 230 1 225 4.83e-155 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22721.t1 AT4G00895 40.945 127 61 3 65 187 75 191 3.20e-19 82.4
MS.gene22721.t1 AT4G09650 29.333 150 99 3 87 230 52 200 5.33e-14 69.3

Find 55 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAGAGAAATGAGATTT+TGG 0.211304 7.4:-743537 None:intergenic
ACTGAGTTTCTGCACACTTT+TGG 0.309140 7.4:-744021 None:intergenic
TCAGTGGTGCAGTGAGGGTT+AGG 0.341774 7.4:+744039 MS.gene22721:CDS
ATGTGGAACTCAAGTTGTTT+TGG 0.349249 7.4:+743953 MS.gene22721:CDS
ATGGTTTGTGTTTGAGAGGT+TGG 0.358792 7.4:-743626 None:intergenic
ATTGGTGAAGATGAAATGTT+TGG 0.381129 7.4:+743993 MS.gene22721:CDS
TGGTTTGTGTTTGAGAGGTT+GGG 0.403119 7.4:-743625 None:intergenic
TTTGAGAGGTTGGGTCTTTG+AGG 0.403625 7.4:-743616 None:intergenic
GCAGCAGCATAGCCAGTAGC+AGG 0.415721 7.4:-743675 None:intergenic
TGAAGGGCATGTGGTTGGTT+TGG 0.422427 7.4:+743851 MS.gene22721:CDS
TGTTGGTGTTGACCCTTCAT+TGG 0.430284 7.4:+743800 MS.gene22721:CDS
TTGAGAGGTTGGGTCTTTGA+GGG 0.430753 7.4:-743615 None:intergenic
ACCACCTTCCTCACCAATGA+AGG 0.445507 7.4:-743813 None:intergenic
TTGTGAAGTGATAGAATTCA+CGG 0.447151 7.4:-743473 None:intergenic
CACCCTTCCCTCGGTTCAAA+GGG 0.453751 7.4:+743725 MS.gene22721:CDS
TTCCATCACAAGCCTGCTAC+TGG 0.468777 7.4:+743663 MS.gene22721:CDS
TTGGTTGTTGAGGATGAGAA+GGG 0.471694 7.4:-743518 None:intergenic
TTTGGTTGTTGAGGATGAGA+AGG 0.473648 7.4:-743519 None:intergenic
ACACCCTTCCCTCGGTTCAA+AGG 0.483662 7.4:+743724 MS.gene22721:CDS
TAGAATTCACGGTTGGTTGT+TGG 0.484183 7.4:-743462 None:intergenic
TTGGTGAAGATGAAATGTTT+GGG 0.494512 7.4:+743994 MS.gene22721:CDS
CAGTGGTGCAGTGAGGGTTA+GGG 0.506987 7.4:+744040 MS.gene22721:CDS
TTGAACCGAGGGAAGGGTGT+TGG 0.514330 7.4:-743721 None:intergenic
TGGGTCTTTGAGGGTAGTGA+AGG 0.516568 7.4:-743606 None:intergenic
AATTCACGGTTGGTTGTTGG+TGG 0.518266 7.4:-743459 None:intergenic
AGCCAGTAGCAGGCTTGTGA+TGG 0.522904 7.4:-743665 None:intergenic
AGGATTTATGATGAATTATG+TGG 0.523605 7.4:+743936 MS.gene22721:CDS
GAAGATGGTTTGTGTTTGAG+AGG 0.530277 7.4:-743630 None:intergenic
TGGAATACTTGAGAAGAAGA+TGG 0.533703 7.4:-743645 None:intergenic
AAATTTGAAGGGCATGTGGT+TGG 0.536206 7.4:+743846 MS.gene22721:CDS
AAGTGTTGGAAGAGTTCATG+AGG 0.536714 7.4:+743916 MS.gene22721:CDS
TGACCCTTCATTGGTGAGGA+AGG 0.538931 7.4:+743809 MS.gene22721:CDS
GTGGAGCAGAGAAAATTTGA+AGG 0.540648 7.4:+743834 MS.gene22721:CDS
GGGAATTGTTGAGGAAGTGT+TGG 0.544791 7.4:+743902 MS.gene22721:CDS
ATCCCTTTGAACCGAGGGAA+GGG 0.545984 7.4:-743727 None:intergenic
GCTGATGAAGAAGAACAAGC+TGG 0.546081 7.4:+743881 MS.gene22721:CDS
AAATTTCAATCAGTTGATGT+TGG 0.559351 7.4:+743783 MS.gene22721:CDS
GAAACTCAGTGGTGCAGTGA+GGG 0.562635 7.4:+744034 MS.gene22721:CDS
AGAGCTTGAGAAAGCATCCA+TGG 0.563193 7.4:-743418 None:intergenic
ATTCACGGTTGGTTGTTGGT+GGG 0.565174 7.4:-743458 None:intergenic
TTCATTGGTGAGGAAGGTGG+TGG 0.567833 7.4:+743815 MS.gene22721:CDS
CATCCCTTTGAACCGAGGGA+AGG 0.585509 7.4:-743728 None:intergenic
CCCTTCATTGGTGAGGAAGG+TGG 0.586166 7.4:+743812 MS.gene22721:CDS
CTGATGAAGAAGAACAAGCT+GGG 0.589597 7.4:+743882 MS.gene22721:CDS
GAGAAAATTTGAAGGGCATG+TGG 0.597790 7.4:+743842 MS.gene22721:CDS
TGAACATCCCTTTGAACCGA+GGG 0.602693 7.4:-743732 None:intergenic
GTGTTGACCCTTCATTGGTG+AGG 0.604863 7.4:+743805 MS.gene22721:CDS
TTGAACATCCCTTTGAACCG+AGG 0.616637 7.4:-743733 None:intergenic
GAAGGGTTTGCAGTTGCATG+AGG 0.616977 7.4:-743501 None:intergenic
GAACAAGCTGGGAATTGTTG+AGG 0.625515 7.4:+743893 MS.gene22721:CDS
CCACCTTCCTCACCAATGAA+GGG 0.627070 7.4:-743812 None:intergenic
GAAGTGATAGAATTCACGGT+TGG 0.666630 7.4:-743469 None:intergenic
TGGAGCAGAGAAAATTTGAA+GGG 0.672649 7.4:+743835 MS.gene22721:CDS
AGAAACTCAGTGGTGCAGTG+AGG 0.695602 7.4:+744033 MS.gene22721:CDS
AAAAGTGTGCAGAAACTCAG+TGG 0.740335 7.4:+744023 MS.gene22721:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGAAAGTTTTTGTTTTTT+GGG - chr7.4:743571-743590 None:intergenic 20.0%
! AGGATTTATGATGAATTATG+TGG + chr7.4:743936-743955 MS.gene22721:CDS 25.0%
! GTTTCATCAAAAAGAGAAAT+TGG + chr7.4:743975-743994 MS.gene22721:CDS 25.0%
!! AAATTTCAATCAGTTGATGT+TGG + chr7.4:743783-743802 MS.gene22721:CDS 25.0%
!! CTGATTGAAATTTCAGTTTT+TGG - chr7.4:743776-743795 None:intergenic 25.0%
!!! ATTGAAATTTCAGTTTTTGG+AGG - chr7.4:743773-743792 None:intergenic 25.0%
!!! GTGAGAAAGTTTTTGTTTTT+TGG - chr7.4:743572-743591 None:intergenic 25.0%
ATTGGTGAAGATGAAATGTT+TGG + chr7.4:743993-744012 MS.gene22721:CDS 30.0%
TTGGTGAAGATGAAATGTTT+GGG + chr7.4:743994-744013 MS.gene22721:CDS 30.0%
TTGTGAAGTGATAGAATTCA+CGG - chr7.4:743476-743495 None:intergenic 30.0%
! TTGGAAGAGAAATGAGATTT+TGG - chr7.4:743540-743559 None:intergenic 30.0%
!!! AAATGAGATTTTGGTTGTTG+AGG - chr7.4:743531-743550 None:intergenic 30.0%
GAGGGTTAGGGTTAAAAATT+TGG + chr7.4:744052-744071 MS.gene22721:CDS 35.0%
TGGAATACTTGAGAAGAAGA+TGG - chr7.4:743648-743667 None:intergenic 35.0%
TGGAGCAGAGAAAATTTGAA+GGG + chr7.4:743835-743854 MS.gene22721:CDS 35.0%
!! ATGTGGAACTCAAGTTGTTT+TGG + chr7.4:743953-743972 MS.gene22721:CDS 35.0%
!!! TGTTTTTTGGGTGACTAAGT+TGG - chr7.4:743559-743578 None:intergenic 35.0%
CTGATGAAGAAGAACAAGCT+GGG + chr7.4:743882-743901 MS.gene22721:CDS 40.0%
GAAGTGATAGAATTCACGGT+TGG - chr7.4:743472-743491 None:intergenic 40.0%
GTGGAGCAGAGAAAATTTGA+AGG + chr7.4:743834-743853 MS.gene22721:CDS 40.0%
TTGGTTGTTGAGGATGAGAA+GGG - chr7.4:743521-743540 None:intergenic 40.0%
TTTGGTTGTTGAGGATGAGA+AGG - chr7.4:743522-743541 None:intergenic 40.0%
! AAAAGTGTGCAGAAACTCAG+TGG + chr7.4:744023-744042 MS.gene22721:CDS 40.0%
! AAATTTGAAGGGCATGTGGT+TGG + chr7.4:743846-743865 MS.gene22721:CDS 40.0%
! ACTGAGTTTCTGCACACTTT+TGG - chr7.4:744024-744043 None:intergenic 40.0%
! ATGGTTTGTGTTTGAGAGGT+TGG - chr7.4:743629-743648 None:intergenic 40.0%
! GAAGATGGTTTGTGTTTGAG+AGG - chr7.4:743633-743652 None:intergenic 40.0%
! GAGAAAATTTGAAGGGCATG+TGG + chr7.4:743842-743861 MS.gene22721:CDS 40.0%
! TAGAATTCACGGTTGGTTGT+TGG - chr7.4:743465-743484 None:intergenic 40.0%
! TGGTTTGTGTTTGAGAGGTT+GGG - chr7.4:743628-743647 None:intergenic 40.0%
!! AAGTGTTGGAAGAGTTCATG+AGG + chr7.4:743916-743935 MS.gene22721:CDS 40.0%
AAAAACCAACACCCTTCCCT+CGG + chr7.4:743716-743735 MS.gene22721:CDS 45.0%
GAACAAGCTGGGAATTGTTG+AGG + chr7.4:743893-743912 MS.gene22721:CDS 45.0%
GCTGATGAAGAAGAACAAGC+TGG + chr7.4:743881-743900 MS.gene22721:CDS 45.0%
TGAACATCCCTTTGAACCGA+GGG - chr7.4:743735-743754 None:intergenic 45.0%
TTGAACATCCCTTTGAACCG+AGG - chr7.4:743736-743755 None:intergenic 45.0%
TTGAGAGGTTGGGTCTTTGA+GGG - chr7.4:743618-743637 None:intergenic 45.0%
TTTGAGAGGTTGGGTCTTTG+AGG - chr7.4:743619-743638 None:intergenic 45.0%
! AATTCACGGTTGGTTGTTGG+TGG - chr7.4:743462-743481 None:intergenic 45.0%
! ATTCACGGTTGGTTGTTGGT+GGG - chr7.4:743461-743480 None:intergenic 45.0%
! GGGAATTGTTGAGGAAGTGT+TGG + chr7.4:743902-743921 MS.gene22721:CDS 45.0%
!! GGTTGTTGGTGGGATTTTGA+GGG - chr7.4:743451-743470 None:intergenic 45.0%
!! TGGTTGTTGGTGGGATTTTG+AGG - chr7.4:743452-743471 None:intergenic 45.0%
!! TGTTGGTGTTGACCCTTCAT+TGG + chr7.4:743800-743819 MS.gene22721:CDS 45.0%
ACCACCTTCCTCACCAATGA+AGG - chr7.4:743816-743835 None:intergenic 50.0%
AGAAACTCAGTGGTGCAGTG+AGG + chr7.4:744033-744052 MS.gene22721:CDS 50.0%
ATCCCTTTGAACCGAGGGAA+GGG - chr7.4:743730-743749 None:intergenic 50.0%
CCACCTTCCTCACCAATGAA+GGG - chr7.4:743815-743834 None:intergenic 50.0%
GAAACTCAGTGGTGCAGTGA+GGG + chr7.4:744034-744053 MS.gene22721:CDS 50.0%
GAAGGGTTTGCAGTTGCATG+AGG - chr7.4:743504-743523 None:intergenic 50.0%
TGACCCTTCATTGGTGAGGA+AGG + chr7.4:743809-743828 MS.gene22721:CDS 50.0%
TTCATTGGTGAGGAAGGTGG+TGG + chr7.4:743815-743834 MS.gene22721:CDS 50.0%
TTCCATCACAAGCCTGCTAC+TGG + chr7.4:743663-743682 MS.gene22721:CDS 50.0%
! GTGTTGACCCTTCATTGGTG+AGG + chr7.4:743805-743824 MS.gene22721:CDS 50.0%
! TGAAGGGCATGTGGTTGGTT+TGG + chr7.4:743851-743870 MS.gene22721:CDS 50.0%
! TGGGTCTTTGAGGGTAGTGA+AGG - chr7.4:743609-743628 None:intergenic 50.0%
ACACCCTTCCCTCGGTTCAA+AGG + chr7.4:743724-743743 MS.gene22721:CDS 55.0%
AGCCAGTAGCAGGCTTGTGA+TGG - chr7.4:743668-743687 None:intergenic 55.0%
CACCCTTCCCTCGGTTCAAA+GGG + chr7.4:743725-743744 MS.gene22721:CDS 55.0%
CAGTGGTGCAGTGAGGGTTA+GGG + chr7.4:744040-744059 MS.gene22721:CDS 55.0%
CATCCCTTTGAACCGAGGGA+AGG - chr7.4:743731-743750 None:intergenic 55.0%
CCCTTCATTGGTGAGGAAGG+TGG + chr7.4:743812-743831 MS.gene22721:CDS 55.0%
TCAGTGGTGCAGTGAGGGTT+AGG + chr7.4:744039-744058 MS.gene22721:CDS 55.0%
! TTGAACCGAGGGAAGGGTGT+TGG - chr7.4:743724-743743 None:intergenic 55.0%
GCAGCAGCATAGCCAGTAGC+AGG - chr7.4:743678-743697 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 743420 744112 743420 ID=MS.gene22721
chr7.4 mRNA 743420 744112 743420 ID=MS.gene22721.t1;Parent=MS.gene22721
chr7.4 exon 743420 744112 743420 ID=MS.gene22721.t1.exon1;Parent=MS.gene22721.t1
chr7.4 CDS 743420 744112 743420 ID=cds.MS.gene22721.t1;Parent=MS.gene22721.t1
Gene Sequence

>MS.gene22721

ATGGATGCTTTCTCAAGCTCTGTTTCAACCCTCAAAATCCCACCAACAACCAACCGTGAATTCTATCACTTCACAAGAACTCCTCATGCAACTGCAAACCCTTCTCATCCTCAACAACCAAAATCTCATTTCTCTTCCAACTTAGTCACCCAAAAAACAAAAACTTTCTCACACAACAATCATTCTCCTTCACTACCCTCAAAGACCCAACCTCTCAAACACAAACCATCTTCTTCTCAAGTATTCCATCACAAGCCTGCTACTGGCTATGCTGCTGCAATCATAGATGTAGCTCAAAAAACCAACACCCTTCCCTCGGTTCAAAGGGATGTTCAAAGACTCTTGAAGTTCCTCCAAAAACTGAAATTTCAATCAGTTGATGTTGGTGTTGACCCTTCATTGGTGAGGAAGGTGGTGGAGCAGAGAAAATTTGAAGGGCATGTGGTTGGTTTGGTGAAGATGCTGATGAAGAAGAACAAGCTGGGAATTGTTGAGGAAGTGTTGGAAGAGTTCATGAGGATTTATGATGAATTATGTGGAACTCAAGTTGTTTTGGTTTCATCAAAAAGAGAAATTGGTGAAGATGAAATGTTTGGGATTGCCAAAAGTGTGCAGAAACTCAGTGGTGCAGTGAGGGTTAGGGTTAAAAATTTGGTTCAAGAAGAGAGTTTTCCTTCTTCTTTTGCAGTGTAA

Protein sequence

>MS.gene22721.t1

MDAFSSSVSTLKIPPTTNREFYHFTRTPHATANPSHPQQPKSHFSSNLVTQKTKTFSHNNHSPSLPSKTQPLKHKPSSSQVFHHKPATGYAAAIIDVAQKTNTLPSVQRDVQRLLKFLQKLKFQSVDVGVDPSLVRKVVEQRKFEGHVVGLVKMLMKKNKLGIVEEVLEEFMRIYDELCGTQVVLVSSKREIGEDEMFGIAKSVQKLSGAVRVRVKNLVQEESFPSSFAV