AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058508

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058508.t1 MTR_5g081230 98.765 324 3 1 1 323 1 324 0.0 658
MS.gene058508.t1 MTR_1g079950 89.404 302 31 1 16 317 30 330 0.0 571
MS.gene058508.t1 MTR_1g079950 89.404 302 31 1 16 317 1 301 0.0 571
MS.gene058508.t1 MTR_1g079950 89.404 302 31 1 16 317 1 301 0.0 570
MS.gene058508.t1 MTR_1g079950 89.404 302 31 1 16 317 1 301 0.0 569
MS.gene058508.t1 MTR_7g116380 87.748 302 36 1 16 317 1 301 0.0 560
MS.gene058508.t1 MTR_7g116380 87.748 302 36 1 16 317 1 301 0.0 560
MS.gene058508.t1 MTR_1g043240 83.946 299 47 1 16 314 1 298 0.0 531
MS.gene058508.t1 MTR_1g074880 81.126 302 56 1 16 317 1 301 0.0 524
MS.gene058508.t1 MTR_1g074880 81.126 302 56 1 16 317 1 301 0.0 523
MS.gene058508.t1 MTR_8g098980 78.105 306 67 0 16 321 1 306 0.0 521
MS.gene058508.t1 MTR_8g005010 78.276 290 62 1 21 310 4 292 5.12e-175 486
MS.gene058508.t1 MTR_7g112870 76.589 299 67 1 16 314 5 300 3.13e-171 478
MS.gene058508.t1 MTR_1g079950 91.322 242 21 0 76 317 34 275 4.20e-171 477
MS.gene058508.t1 MTR_1g074880 83.128 243 41 0 75 317 4 246 1.58e-157 441
MS.gene058508.t1 MTR_1g074880 83.128 243 41 0 75 317 4 246 2.08e-157 441
MS.gene058508.t1 MTR_8g062430 45.763 295 153 3 23 316 6 294 4.29e-95 284
MS.gene058508.t1 MTR_5g093990 44.108 297 160 2 21 316 1 292 2.08e-94 282
MS.gene058508.t1 MTR_7g107310 44.595 296 157 3 20 314 10 299 4.07e-94 282
MS.gene058508.t1 MTR_5g037200 46.102 295 152 3 23 316 6 294 4.60e-94 281
MS.gene058508.t1 MTR_8g085610 46.350 274 145 2 42 314 22 294 6.01e-92 276
MS.gene058508.t1 MTR_1g039380 42.657 286 161 3 33 316 7 291 8.95e-90 270
MS.gene058508.t1 MTR_8g085610 45.556 270 145 2 46 314 2 270 1.21e-89 270
MS.gene058508.t1 MTR_1g039380 43.939 264 145 3 55 316 5 267 5.16e-87 263
MS.gene058508.t1 MTR_2g087530 46.006 313 153 6 14 310 521 833 6.80e-83 268
MS.gene058508.t1 MTR_2g087530 46.006 313 153 6 14 310 634 946 2.08e-82 269
MS.gene058508.t1 MTR_1g089370 48.239 284 131 6 42 310 548 830 2.21e-81 264
MS.gene058508.t1 MTR_4g050540 45.687 313 154 6 14 310 639 951 4.65e-81 265
MS.gene058508.t1 MTR_3g452710 40.476 294 164 5 8 295 158 446 7.35e-71 228
MS.gene058508.t1 MTR_3g452710 40.476 294 164 5 8 295 225 513 4.04e-70 228
MS.gene058508.t1 MTR_2g100130 37.662 308 173 7 2 295 63 365 2.11e-67 216
MS.gene058508.t1 MTR_2g100130 37.662 308 173 7 2 295 133 435 1.57e-66 216
MS.gene058508.t1 MTR_2g100130 37.662 308 173 7 2 295 172 474 3.22e-66 216
MS.gene058508.t1 MTR_2g100130 37.662 308 173 7 2 295 162 464 3.25e-66 216
MS.gene058508.t1 MTR_2g100130 37.662 308 173 7 2 295 172 474 3.63e-66 216
MS.gene058508.t1 MTR_2g100130 37.662 308 172 8 2 295 133 434 2.59e-65 213
MS.gene058508.t1 MTR_2g100130 37.662 308 172 8 2 295 172 473 5.46e-65 213
MS.gene058508.t1 MTR_2g100130 37.919 298 166 7 2 285 172 464 1.44e-64 212
MS.gene058508.t1 MTR_2g100160 43.548 248 132 5 55 295 138 384 1.89e-60 199
MS.gene058508.t1 MTR_5g037200 51.534 163 78 1 42 203 20 182 9.99e-58 186
MS.gene058508.t1 MTR_8g072600 31.613 310 151 9 69 317 129 438 2.64e-36 136
MS.gene058508.t1 MTR_8g072600 33.184 223 114 5 68 255 128 350 4.84e-29 114
MS.gene058508.t1 MTR_2g099710 28.896 308 167 13 47 313 211 507 2.83e-21 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058508.t1 AT2G39840 91.262 309 27 0 15 323 13 321 0.0 599
MS.gene058508.t1 AT5G59160 82.132 319 49 2 1 318 1 312 0.0 550
MS.gene058508.t1 AT5G59160 82.132 319 49 2 1 318 1 312 0.0 550
MS.gene058508.t1 AT5G59160 82.132 319 49 2 1 318 1 312 0.0 550
MS.gene058508.t1 AT2G29400 81.329 316 55 3 6 318 4 318 0.0 541
MS.gene058508.t1 AT3G46820 78.616 318 60 2 1 317 1 311 0.0 531
MS.gene058508.t1 AT1G64040 79.801 302 61 0 16 317 1 302 0.0 519
MS.gene058508.t1 AT4G11240 76.623 308 72 0 16 323 1 308 0.0 513
MS.gene058508.t1 AT5G43380 77.850 307 67 1 16 322 1 306 1.82e-180 502
MS.gene058508.t1 AT5G43380 77.850 307 67 1 16 322 1 306 5.14e-180 500
MS.gene058508.t1 AT5G43380 77.850 307 67 1 16 322 1 306 1.27e-179 499
MS.gene058508.t1 AT5G27840 77.104 297 65 1 15 311 8 301 2.82e-174 486
MS.gene058508.t1 AT5G27840 77.104 297 65 1 15 311 8 301 3.61e-174 486
MS.gene058508.t1 AT5G27840 77.104 297 65 1 15 311 8 301 3.61e-174 486
MS.gene058508.t1 AT5G27840 77.104 297 65 1 15 311 8 301 5.46e-174 485
MS.gene058508.t1 AT3G05580 76.768 297 66 1 15 311 8 301 5.93e-170 474
MS.gene058508.t1 AT3G05580 75.806 248 57 1 15 262 8 252 3.86e-137 389
MS.gene058508.t1 AT5G55260 46.099 282 151 1 42 322 17 298 1.04e-96 288
MS.gene058508.t1 AT1G10430 45.763 295 153 3 23 316 6 294 1.33e-95 285
MS.gene058508.t1 AT1G10430 45.763 295 153 3 23 316 6 294 1.33e-95 285
MS.gene058508.t1 AT4G26720 42.761 297 164 2 21 316 1 292 2.34e-95 285
MS.gene058508.t1 AT2G42500 47.445 274 142 2 42 314 27 299 5.95e-95 284
MS.gene058508.t1 AT1G69960 47.101 276 144 2 42 316 21 295 3.79e-94 282
MS.gene058508.t1 AT3G58500 46.715 274 144 2 42 314 27 299 2.40e-93 280
MS.gene058508.t1 AT1G59830 44.746 295 156 3 23 316 6 294 6.48e-93 279
MS.gene058508.t1 AT3G19980 42.657 286 161 3 33 316 7 291 3.49e-89 269
MS.gene058508.t1 AT1G50370 42.308 286 162 3 33 316 7 291 3.85e-89 269
MS.gene058508.t1 AT5G55260 40.248 323 151 2 42 322 17 339 4.11e-88 268
MS.gene058508.t1 AT4G03080 45.031 322 159 7 6 310 512 832 3.19e-83 269
MS.gene058508.t1 AT1G59830 47.177 248 124 3 23 269 6 247 7.69e-81 246
MS.gene058508.t1 AT2G27210 48.029 279 131 5 46 310 674 952 7.07e-80 262
MS.gene058508.t1 AT2G27210 48.029 279 131 5 46 310 679 957 7.45e-80 262
MS.gene058508.t1 AT1G03445 47.887 284 131 8 42 310 476 757 1.40e-79 258
MS.gene058508.t1 AT1G03445 47.887 284 131 8 42 310 476 757 1.54e-79 258
MS.gene058508.t1 AT1G08420 45.048 313 156 6 14 310 656 968 3.65e-79 260
MS.gene058508.t1 AT1G08420 45.048 313 156 6 14 310 651 963 4.04e-79 260
MS.gene058508.t1 AT2G42500 48.869 221 111 2 95 314 33 252 8.59e-75 231
MS.gene058508.t1 AT2G42810 41.156 294 162 5 8 295 160 448 3.28e-69 223
MS.gene058508.t1 AT2G42810 41.156 294 162 5 8 295 160 448 3.28e-69 223
MS.gene058508.t1 AT2G42810 41.156 294 162 5 8 295 160 448 3.28e-69 223
MS.gene058508.t1 AT2G42810 41.156 294 162 5 8 295 214 502 8.28e-69 224
MS.gene058508.t1 AT2G42810 41.156 294 162 5 8 295 213 501 8.64e-69 224
MS.gene058508.t1 AT2G42500 45.578 147 76 3 42 184 27 173 8.28e-43 146
MS.gene058508.t1 AT2G42500 45.752 153 77 4 42 190 27 177 1.38e-42 145
MS.gene058508.t1 AT2G42500 47.482 139 69 3 42 176 27 165 4.89e-42 144
MS.gene058508.t1 AT5G63870 30.744 309 151 11 71 317 79 386 2.33e-32 124
MS.gene058508.t1 AT5G63870 30.744 309 151 11 71 317 79 386 2.33e-32 124
MS.gene058508.t1 AT5G63870 33.032 221 111 7 71 255 79 298 1.75e-26 106
MS.gene058508.t1 AT1G48120 29.195 298 151 8 47 284 628 925 2.17e-26 110
MS.gene058508.t1 AT5G10900 29.851 268 140 9 68 293 222 483 2.12e-20 92.4
MS.gene058508.t1 AT5G10900 29.851 268 140 9 68 293 222 483 2.73e-20 92.0
MS.gene058508.t1 AT5G10900 29.851 268 140 9 68 293 222 483 2.99e-20 91.7

Find 77 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCCTGACCTCACAAATT+TGG 0.198743 5.3:+65625395 MS.gene058508:CDS
GCTTCAAGTTCAAGCAAATT+AGG 0.250559 5.3:-65624268 None:intergenic
GATAACTGAACTTGCTTTCC+AGG 0.253465 5.3:-65624190 None:intergenic
TTGTTAAGGCTCTTTGAGTA+TGG 0.289359 5.3:+65625081 MS.gene058508:CDS
GACGAGGCAGATTCCGGATT+TGG 0.290635 5.3:-65625419 None:intergenic
AATATACCACAAATCTTAAT+AGG 0.294724 5.3:-65624292 None:intergenic
TCTCTGCAGGCTTAAGAATC+TGG 0.306156 5.3:-65625859 None:intergenic
ACGAGTGCGCTTCAATTAAT+AGG 0.308425 5.3:+65625241 MS.gene058508:CDS
GCTTCAATTAATAGGATATA+TGG 0.334197 5.3:+65625249 MS.gene058508:CDS
TCAAATTCACCGCAGTAGTT+TGG 0.338300 5.3:-65625788 None:intergenic
AGAGGAGTGTCCTACACTTT+TGG 0.354737 5.3:+65625528 MS.gene058508:CDS
GTTTGCTCTGTGATTTGCTT+TGG 0.359634 5.3:+65625463 MS.gene058508:CDS
CCTTATTTGTCGTGCTCATC+AGG 0.365242 5.3:+65625593 MS.gene058508:CDS
TGGTCCAAATTTGTGAGGTC+AGG 0.377025 5.3:-65625399 None:intergenic
GCTTTCCAGGTCGAGATAAT+CGG 0.389712 5.3:-65624177 None:intergenic
GAACTGGACGAGGCAGATTC+CGG 0.393173 5.3:-65625425 None:intergenic
ACCTGAGCTGGTTGCGGTTG+TGG 0.398008 5.3:-65624106 None:intergenic
TGGTAAAGATGTGAAGGGTT+GGG 0.416189 5.3:+65625494 MS.gene058508:CDS
GAAGGTTTAACGTGAGGCTT+TGG 0.417843 5.3:+65625292 MS.gene058508:CDS
GAAGCTCCTATTAAGATTTG+TGG 0.437377 5.3:+65624286 MS.gene058508:CDS
GGCAGTACAGTGATTTGTTA+AGG 0.439229 5.3:+65625067 MS.gene058508:CDS
TGTTAAGGCTCTTTGAGTAT+GGG 0.442492 5.3:+65625082 MS.gene058508:CDS
TCACAAATTTGGACCAAATC+CGG 0.443187 5.3:+65625406 MS.gene058508:CDS
CCTGATGAGCACGACAAATA+AGG 0.445699 5.3:-65625593 None:intergenic
TTTCTACAGGTTGTAGAGGA+TGG 0.456508 5.3:+65625719 MS.gene058508:intron
ATAAGCAAGCAATAGACAAA+TGG 0.460318 5.3:-65625175 None:intergenic
GATTTGCTTTGGTCTGATCC+TGG 0.463579 5.3:+65625474 MS.gene058508:CDS
AGCAAACCAGTGTCGGGAAT+AGG 0.469815 5.3:-65625447 None:intergenic
GTTAAGGCTCTTTGAGTATG+GGG 0.473187 5.3:+65625083 MS.gene058508:CDS
CCAGTGTCGGGAATAGGAAC+TGG 0.483717 5.3:-65625441 None:intergenic
GGTAAAGATGTGAAGGGTTG+GGG 0.485097 5.3:+65625495 MS.gene058508:CDS
ATCGGACCTCCGTGAGTCGC+CGG 0.486009 5.3:-65624159 None:intergenic
CGGTGTTGGATGACATTATC+CGG 0.486367 5.3:+65624140 MS.gene058508:CDS
CAACCCTTCACATCTTTACC+AGG 0.486888 5.3:-65625492 None:intergenic
CTGGTAAAGATGTGAAGGGT+TGG 0.489081 5.3:+65625493 MS.gene058508:CDS
ACTCGTGATTTCCCCTCAAA+AGG 0.489322 5.3:-65625224 None:intergenic
TCACAGAGCAAACCAGTGTC+GGG 0.491919 5.3:-65625453 None:intergenic
ACCACAACCGCAACCAGCTC+AGG 0.495833 5.3:+65624105 MS.gene058508:CDS
TCAATCACCTGAGCTGGTTG+CGG 0.503443 5.3:-65624112 None:intergenic
TGCGGTGAATTTGACAATGC+TGG 0.504127 5.3:+65625797 MS.gene058508:CDS
GGTTGGGGCATGAATGATAG+AGG 0.507092 5.3:+65625510 MS.gene058508:CDS
TTCTACAGGTTGTAGAGGAT+GGG 0.512314 5.3:+65625720 MS.gene058508:intron
GGTGATTGATGAGGCGGTGT+TGG 0.514805 5.3:+65624126 MS.gene058508:CDS
GCCTCATCAATCACCTGAGC+TGG 0.516629 5.3:-65624118 None:intergenic
GGTCCAAATTTGTGAGGTCA+GGG 0.517305 5.3:-65625398 None:intergenic
GGTATGAATTCTTTGCAGAC+AGG 0.527480 5.3:+65625741 MS.gene058508:CDS
ATCACAGAGCAAACCAGTGT+CGG 0.531106 5.3:-65625454 None:intergenic
GCAGTTAAAAGATTCTGTAA+AGG 0.537513 5.3:-65625319 None:intergenic
CCAGTTCCTATTCCCGACAC+TGG 0.551030 5.3:+65625441 MS.gene058508:CDS
TTAAGGCTCTTTGAGTATGG+GGG 0.557869 5.3:+65625084 MS.gene058508:CDS
TGATCCTGGTAAAGATGTGA+AGG 0.568216 5.3:+65625488 MS.gene058508:CDS
TAGGGGACTATGTCGATCGT+GGG 0.581599 5.3:+65625136 MS.gene058508:CDS
TTAGGGGACTATGTCGATCG+TGG 0.584691 5.3:+65625135 MS.gene058508:CDS
AGCTCAGGTGATTGATGAGG+CGG 0.591360 5.3:+65624120 MS.gene058508:CDS
AAAATTCAGCCACCTTATCA+GGG 0.604962 5.3:-65625551 None:intergenic
AATCTTTCTACAGGTTGTAG+AGG 0.609590 5.3:+65625715 MS.gene058508:intron
CTTATCAGGGCCAAAAGTGT+AGG 0.611607 5.3:-65625538 None:intergenic
TGATGTCAAACAAGATGTGA+TGG 0.614410 5.3:+65625897 MS.gene058508:CDS
TCATCTATAAGAGCTGCCAC+AGG 0.614413 5.3:-65625345 None:intergenic
GAGAAAATATTGTGCATGCA+TGG 0.617309 5.3:+65625366 MS.gene058508:CDS
GTCCAAATTTGTGAGGTCAG+GGG 0.618457 5.3:-65625397 None:intergenic
AAGAGATAATTAGACTGAGG+AGG 0.620172 5.3:-65625111 None:intergenic
GGATTTGGTCCAAATTTGTG+AGG 0.622555 5.3:-65625404 None:intergenic
TCGGGAATAGGAACTGGACG+AGG 0.628912 5.3:-65625435 None:intergenic
TGAGCTGGTTGCGGTTGTGG+TGG 0.632704 5.3:-65624103 None:intergenic
TTTATGATGAATGTAAGCGA+AGG 0.637254 5.3:+65625274 MS.gene058508:CDS
TGACATTATCCGGCGACTCA+CGG 0.637466 5.3:+65624150 MS.gene058508:CDS
CTATAAGAGCTGCCACAGGA+AGG 0.639489 5.3:-65625341 None:intergenic
GAGGTCCGATTATCTCGACC+TGG 0.644071 5.3:+65624172 MS.gene058508:CDS
TGCAGGCTTAAGAATCTGGA+AGG 0.647935 5.3:-65625855 None:intergenic
GTAAGCGAAGGTTTAACGTG+AGG 0.650857 5.3:+65625286 MS.gene058508:CDS
TCAGAATCAATGAGTGCACA+AGG 0.654048 5.3:+65624076 None:intergenic
ACCAGCTCAGGTGATTGATG+AGG 0.656427 5.3:+65624117 MS.gene058508:CDS
TGCTGGTGCGATGATGAGTG+TGG 0.671932 5.3:+65625814 MS.gene058508:CDS
GATCCTGGTAAAGATGTGAA+GGG 0.727062 5.3:+65625489 MS.gene058508:CDS
AAAATATTGTGCATGCATGG+TGG 0.745802 5.3:+65625369 MS.gene058508:CDS
CATTATCCGGCGACTCACGG+AGG 0.795356 5.3:+65624153 MS.gene058508:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAAAAAAATCAAAAT+GGG - chr5.3:65624342-65624361 None:intergenic 10.0%
!! AGTAATAAAAATTATCAAAT+TGG - chr5.3:65624704-65624723 None:intergenic 10.0%
!! GTAAAATAAAAGATAAAAAA+AGG - chr5.3:65624674-65624693 None:intergenic 10.0%
!! GTAATAAAAATTATCAAATT+GGG - chr5.3:65624703-65624722 None:intergenic 10.0%
!! TAAAAACAAAAAAATCAAAA+TGG - chr5.3:65624343-65624362 None:intergenic 10.0%
!! TAATAAAAATTATCAAATTG+GGG - chr5.3:65624702-65624721 None:intergenic 10.0%
!!! ATTTGATAATTTTTATTACT+AGG + chr5.3:65624704-65624723 MS.gene058508:intron 10.0%
!!! ATTTTGATTTTTTTGTTTTT+AGG + chr5.3:65624342-65624361 MS.gene058508:intron 10.0%
!!! TTTGATAATTTTTATTACTA+GGG + chr5.3:65624705-65624724 MS.gene058508:intron 10.0%
!!! TTTTAAATATTCATGTTATT+GGG + chr5.3:65624628-65624647 MS.gene058508:intron 10.0%
!!! TTTTGATTTTTTTGTTTTTA+GGG + chr5.3:65624343-65624362 MS.gene058508:intron 10.0%
!!! TTTTTAAATATTCATGTTAT+TGG + chr5.3:65624627-65624646 MS.gene058508:intron 10.0%
!! AAAACAAAAAAATCAAAATG+GGG - chr5.3:65624341-65624360 None:intergenic 15.0%
!! AAGAATTGAATAATAAAATG+TGG + chr5.3:65624824-65624843 MS.gene058508:intron 15.0%
!! AGAATTGAATAATAAAATGT+GGG + chr5.3:65624825-65624844 MS.gene058508:intron 15.0%
!!! TTTCTTTTTAATGTTGTATT+TGG + chr5.3:65624739-65624758 MS.gene058508:intron 15.0%
!! AATATACCACAAATCTTAAT+AGG - chr5.3:65624295-65624314 None:intergenic 20.0%
!! AATATACCCTAAAATTCAAA+AGG - chr5.3:65624792-65624811 None:intergenic 20.0%
!!! AAACTTTCTGAAAACTAAAA+GGG - chr5.3:65624854-65624873 None:intergenic 20.0%
!!! AATGTACTATTTTATTAGTC+TGG + chr5.3:65624996-65625015 MS.gene058508:intron 20.0%
!!! AGTCTAATTATCTCTTTTTA+GGG + chr5.3:65625118-65625137 MS.gene058508:CDS 20.0%
!!! TTAGAACCTTTTGAATTTTA+GGG + chr5.3:65624783-65624802 MS.gene058508:intron 20.0%
!!! TTTGAATTTTAGGGTATATT+TGG + chr5.3:65624792-65624811 MS.gene058508:intron 20.0%
! AAAAAGAGATAATTAGACTG+AGG - chr5.3:65625117-65625136 None:intergenic 25.0%
! GCTTCAATTAATAGGATATA+TGG + chr5.3:65625249-65625268 MS.gene058508:CDS 25.0%
! TAAAAATTATCAAATTGGGG+TGG - chr5.3:65624699-65624718 None:intergenic 25.0%
!! AAAATCCTGTTTGAATTTTC+AGG + chr5.3:65625033-65625052 MS.gene058508:intron 25.0%
!! CAAACTTTCTGAAAACTAAA+AGG - chr5.3:65624855-65624874 None:intergenic 25.0%
!! CAGTCTAATTATCTCTTTTT+AGG + chr5.3:65625117-65625136 MS.gene058508:CDS 25.0%
!! GTCTAATTATCTCTTTTTAG+GGG + chr5.3:65625119-65625138 MS.gene058508:CDS 25.0%
!! GTTAGAACCTTTTGAATTTT+AGG + chr5.3:65624782-65624801 MS.gene058508:intron 25.0%
!!! AAAAGGTACCCAAAAAATTA+AGG - chr5.3:65624657-65624676 None:intergenic 25.0%
!!! AATGGCTTTTTTTAGTTGTT+TGG + chr5.3:65624371-65624390 MS.gene058508:intron 25.0%
!!! ACTATTTTATTAGTCTGGTA+TGG + chr5.3:65625001-65625020 MS.gene058508:intron 25.0%
!!! AGTTTGAAGTTTTTGGTTTT+AGG + chr5.3:65624422-65624441 MS.gene058508:intron 25.0%
!!! ATTGGGTATCCTTAATTTTT+TGG + chr5.3:65624645-65624664 MS.gene058508:intron 25.0%
!!! CTTTTAGTTTTCAGAAAGTT+TGG + chr5.3:65624853-65624872 MS.gene058508:intron 25.0%
!!! GTGTATTTGATTTGATTGTT+AGG + chr5.3:65624572-65624591 MS.gene058508:intron 25.0%
!!! GTTTGAAGTTTTTGGTTTTA+GGG + chr5.3:65624423-65624442 MS.gene058508:intron 25.0%
!!! TGAAAACTTTTTCCTTTTGA+GGG + chr5.3:65625212-65625231 MS.gene058508:CDS 25.0%
!!! TTATTTTTAAGTGTGTGTTG+TGG + chr5.3:65624965-65624984 MS.gene058508:intron 25.0%
!!! TTGGGTATCCTTAATTTTTT+GGG + chr5.3:65624646-65624665 MS.gene058508:intron 25.0%
!!! TTTAGTTGTTTGGTGATTTT+GGG + chr5.3:65624381-65624400 MS.gene058508:intron 25.0%
!!! TTTGTTTTTAGGGTTTAGAA+TGG + chr5.3:65624353-65624372 MS.gene058508:intron 25.0%
!!! TTTTACCTTCTTTGAGAAAA+AGG + chr5.3:65625656-65625675 MS.gene058508:intron 25.0%
!!! TTTTAGTTGTTTGGTGATTT+TGG + chr5.3:65624380-65624399 MS.gene058508:intron 25.0%
AGGGATTGAAAAAGAAAATG+TGG + chr5.3:65624479-65624498 MS.gene058508:intron 30.0%
ATAAGCAAGCAATAGACAAA+TGG - chr5.3:65625178-65625197 None:intergenic 30.0%
GCAGTTAAAAGATTCTGTAA+AGG - chr5.3:65625322-65625341 None:intergenic 30.0%
TGATACTGTAATCTTTCTAC+AGG + chr5.3:65625706-65625725 MS.gene058508:intron 30.0%
TTAGGGTATATTTGGTTGTT+TGG + chr5.3:65624800-65624819 MS.gene058508:intron 30.0%
TTTATGATGAATGTAAGCGA+AGG + chr5.3:65625274-65625293 MS.gene058508:CDS 30.0%
! ACTTTTCTGCAAGTGTTTAT+TGG + chr5.3:65624904-65624923 MS.gene058508:intron 30.0%
! CTCAAAAGGAAAAAGTTTTC+AGG - chr5.3:65625213-65625232 None:intergenic 30.0%
! CTCTTTTTACGAAGGTTTTA+GGG + chr5.3:65624450-65624469 MS.gene058508:intron 30.0%
! TAGTTTTCAGAAAGTTTGGA+GGG + chr5.3:65624857-65624876 MS.gene058508:intron 30.0%
! TCTCTTTTTACGAAGGTTTT+AGG + chr5.3:65624449-65624468 MS.gene058508:intron 30.0%
!! CTAAACTTGTGTTGCTTTAA+TGG + chr5.3:65625679-65625698 MS.gene058508:intron 30.0%
!! CTGAAAACTTTTTCCTTTTG+AGG + chr5.3:65625211-65625230 MS.gene058508:CDS 30.0%
!! GAAAACTTTTTCCTTTTGAG+GGG + chr5.3:65625213-65625232 MS.gene058508:CDS 30.0%
!! GTTTTAATGTTCACGCTTTT+GGG + chr5.3:65624507-65624526 MS.gene058508:intron 30.0%
!! TGTTGCAAGTTTGAAGTTTT+TGG + chr5.3:65624415-65624434 MS.gene058508:intron 30.0%
!! TTAGTTTTCAGAAAGTTTGG+AGG + chr5.3:65624856-65624875 MS.gene058508:intron 30.0%
!! TTTAGCCTTTTTCTCAAAGA+AGG - chr5.3:65625664-65625683 None:intergenic 30.0%
!!! AATTTTCAGGTGACATTCAT+GGG + chr5.3:65625046-65625065 MS.gene058508:intron 30.0%
!!! TGTTTTAATGTTCACGCTTT+TGG + chr5.3:65624506-65624525 MS.gene058508:intron 30.0%
AAAAATTCAGCCACCTTATC+AGG - chr5.3:65625555-65625574 None:intergenic 35.0%
AAAATATTGTGCATGCATGG+TGG + chr5.3:65625369-65625388 MS.gene058508:CDS 35.0%
AAAATTCAGCCACCTTATCA+GGG - chr5.3:65625554-65625573 None:intergenic 35.0%
AAGAGATAATTAGACTGAGG+AGG - chr5.3:65625114-65625133 None:intergenic 35.0%
AATCTTTCTACAGGTTGTAG+AGG + chr5.3:65625715-65625734 MS.gene058508:intron 35.0%
AGAAGGTAAAAGAAATGACG+TGG - chr5.3:65625647-65625666 None:intergenic 35.0%
GAAGCTCCTATTAAGATTTG+TGG + chr5.3:65624286-65624305 MS.gene058508:CDS 35.0%
GAGAAAATATTGTGCATGCA+TGG + chr5.3:65625366-65625385 MS.gene058508:CDS 35.0%
TCACAAATTTGGACCAAATC+CGG + chr5.3:65625406-65625425 MS.gene058508:CDS 35.0%
TGTCACCTGAAAATTCAAAC+AGG - chr5.3:65625041-65625060 None:intergenic 35.0%
TGTTAAGGCTCTTTGAGTAT+GGG + chr5.3:65625082-65625101 MS.gene058508:CDS 35.0%
TTGTTAAGGCTCTTTGAGTA+TGG + chr5.3:65625081-65625100 MS.gene058508:CDS 35.0%
! AGGGTTTTCTCTTTTTACGA+AGG + chr5.3:65624442-65624461 MS.gene058508:intron 35.0%
! ATTTTTGACAAGGCATGACT+TGG + chr5.3:65625569-65625588 MS.gene058508:CDS 35.0%
! CAAAATGGGGTGTCAAAAAA+GGG - chr5.3:65624328-65624347 None:intergenic 35.0%
! GCTTCAAGTTCAAGCAAATT+AGG - chr5.3:65624271-65624290 None:intergenic 35.0%
! GCTTGATTTGAAGCTAAGAA+TGG + chr5.3:65624533-65624552 MS.gene058508:intron 35.0%
! TCAAAATGGGGTGTCAAAAA+AGG - chr5.3:65624329-65624348 None:intergenic 35.0%
!! AGACTTGTTGATTGCTGTTA+AGG + chr5.3:65624942-65624961 MS.gene058508:intron 35.0%
!!! AACTTTGTTTTCTTCTCTGC+AGG - chr5.3:65625875-65625894 None:intergenic 35.0%
!!! GAATTTTCAGGTGACATTCA+TGG + chr5.3:65625045-65625064 MS.gene058508:intron 35.0%
!!! TTTACGAAGGTTTTAGGGTA+TGG + chr5.3:65624455-65624474 MS.gene058508:intron 35.0%
AAAAGAAATGACGTGGAGGA+GGG - chr5.3:65625640-65625659 None:intergenic 40.0%
ACGAGTGCGCTTCAATTAAT+AGG + chr5.3:65625241-65625260 MS.gene058508:CDS 40.0%
AGGTAAAAGAAATGACGTGG+AGG - chr5.3:65625644-65625663 None:intergenic 40.0%
GATCCTGGTAAAGATGTGAA+GGG + chr5.3:65625489-65625508 MS.gene058508:CDS 40.0%
GGTATGAATTCTTTGCAGAC+AGG + chr5.3:65625741-65625760 MS.gene058508:CDS 40.0%
GTTAAGGCTCTTTGAGTATG+GGG + chr5.3:65625083-65625102 MS.gene058508:CDS 40.0%
TAAAAGAAATGACGTGGAGG+AGG - chr5.3:65625641-65625660 None:intergenic 40.0%
TCAAATTCACCGCAGTAGTT+TGG - chr5.3:65625791-65625810 None:intergenic 40.0%
TGATCCTGGTAAAGATGTGA+AGG + chr5.3:65625488-65625507 MS.gene058508:CDS 40.0%
TGGTAAAGATGTGAAGGGTT+GGG + chr5.3:65625494-65625513 MS.gene058508:CDS 40.0%
TTAAGGCTCTTTGAGTATGG+GGG + chr5.3:65625084-65625103 MS.gene058508:CDS 40.0%
TTCTACAGGTTGTAGAGGAT+GGG + chr5.3:65625720-65625739 MS.gene058508:intron 40.0%
TTTCTACAGGTTGTAGAGGA+TGG + chr5.3:65625719-65625738 MS.gene058508:intron 40.0%
! AGGTGGCTGAATTTTTGACA+AGG + chr5.3:65625559-65625578 MS.gene058508:CDS 40.0%
! GATAACTGAACTTGCTTTCC+AGG - chr5.3:65624193-65624212 None:intergenic 40.0%
! GGATTTGGTCCAAATTTGTG+AGG - chr5.3:65625407-65625426 None:intergenic 40.0%
! TTTTCAGCTCCAAACTACTG+CGG + chr5.3:65625779-65625798 MS.gene058508:CDS 40.0%
!! ATCTTTTAACTGCCTTCCTG+TGG + chr5.3:65625329-65625348 MS.gene058508:CDS 40.0%
!! GGCAGTACAGTGATTTGTTA+AGG + chr5.3:65625067-65625086 MS.gene058508:CDS 40.0%
!! GTTTGCTCTGTGATTTGCTT+TGG + chr5.3:65625463-65625482 MS.gene058508:CDS 40.0%
!!! GAAGGTTTTAGGGTATGGTA+GGG + chr5.3:65624460-65624479 MS.gene058508:intron 40.0%
ACTCGTGATTTCCCCTCAAA+AGG - chr5.3:65625227-65625246 None:intergenic 45.0%
ATCACAGAGCAAACCAGTGT+CGG - chr5.3:65625457-65625476 None:intergenic 45.0%
CAACCCTTCACATCTTTACC+AGG - chr5.3:65625495-65625514 None:intergenic 45.0%
CCTGATGAGCACGACAAATA+AGG - chr5.3:65625596-65625615 None:intergenic 45.0%
CTGGTAAAGATGTGAAGGGT+TGG + chr5.3:65625493-65625512 MS.gene058508:CDS 45.0%
GAAGGTTTAACGTGAGGCTT+TGG + chr5.3:65625292-65625311 MS.gene058508:CDS 45.0%
GCTTTCCAGGTCGAGATAAT+CGG - chr5.3:65624180-65624199 None:intergenic 45.0%
GGTAAAGATGTGAAGGGTTG+GGG + chr5.3:65625495-65625514 MS.gene058508:CDS 45.0%
GGTCCAAATTTGTGAGGTCA+GGG - chr5.3:65625401-65625420 None:intergenic 45.0%
GTAAGCGAAGGTTTAACGTG+AGG + chr5.3:65625286-65625305 MS.gene058508:CDS 45.0%
GTCCAAATTTGTGAGGTCAG+GGG - chr5.3:65625400-65625419 None:intergenic 45.0%
TCATCTATAAGAGCTGCCAC+AGG - chr5.3:65625348-65625367 None:intergenic 45.0%
TCTCTGCAGGCTTAAGAATC+TGG - chr5.3:65625862-65625881 None:intergenic 45.0%
TGCAGGCTTAAGAATCTGGA+AGG - chr5.3:65625858-65625877 None:intergenic 45.0%
TGCGGTGAATTTGACAATGC+TGG + chr5.3:65625797-65625816 MS.gene058508:CDS 45.0%
TGGTCCAAATTTGTGAGGTC+AGG - chr5.3:65625402-65625421 None:intergenic 45.0%
TTCCCCTGACCTCACAAATT+TGG + chr5.3:65625395-65625414 MS.gene058508:CDS 45.0%
! CCTTATTTGTCGTGCTCATC+AGG + chr5.3:65625593-65625612 MS.gene058508:CDS 45.0%
! CGGTGTTGGATGACATTATC+CGG + chr5.3:65624140-65624159 MS.gene058508:CDS 45.0%
! CTACACTTTTGGCCCTGATA+AGG + chr5.3:65625539-65625558 MS.gene058508:CDS 45.0%
! CTTATCAGGGCCAAAAGTGT+AGG - chr5.3:65625541-65625560 None:intergenic 45.0%
! GATTTGCTTTGGTCTGATCC+TGG + chr5.3:65625474-65625493 MS.gene058508:CDS 45.0%
!!! AGAGGAGTGTCCTACACTTT+TGG + chr5.3:65625528-65625547 MS.gene058508:CDS 45.0%
!!! CGAAGGTTTTAGGGTATGGT+AGG + chr5.3:65624459-65624478 MS.gene058508:intron 45.0%
ACCAGCTCAGGTGATTGATG+AGG + chr5.3:65624117-65624136 MS.gene058508:CDS 50.0%
AGCTCAGGTGATTGATGAGG+CGG + chr5.3:65624120-65624139 MS.gene058508:CDS 50.0%
CTATAAGAGCTGCCACAGGA+AGG - chr5.3:65625344-65625363 None:intergenic 50.0%
GGTTGGGGCATGAATGATAG+AGG + chr5.3:65625510-65625529 MS.gene058508:CDS 50.0%
TAGGGGACTATGTCGATCGT+GGG + chr5.3:65625136-65625155 MS.gene058508:CDS 50.0%
TCAATCACCTGAGCTGGTTG+CGG - chr5.3:65624115-65624134 None:intergenic 50.0%
TCACAGAGCAAACCAGTGTC+GGG - chr5.3:65625456-65625475 None:intergenic 50.0%
TGACATTATCCGGCGACTCA+CGG + chr5.3:65624150-65624169 MS.gene058508:CDS 50.0%
TTAGGGGACTATGTCGATCG+TGG + chr5.3:65625135-65625154 MS.gene058508:CDS 50.0%
! AGCAAACCAGTGTCGGGAAT+AGG - chr5.3:65625450-65625469 None:intergenic 50.0%
! CACTTTTGGCCCTGATAAGG+TGG + chr5.3:65625542-65625561 MS.gene058508:CDS 50.0%
AGAAATGACGTGGAGGAGGG+AGG - chr5.3:65625637-65625656 None:intergenic 55.0%
CCAGTTCCTATTCCCGACAC+TGG + chr5.3:65625441-65625460 MS.gene058508:CDS 55.0%
GAACTGGACGAGGCAGATTC+CGG - chr5.3:65625428-65625447 None:intergenic 55.0%
GACGAGGCAGATTCCGGATT+TGG - chr5.3:65625422-65625441 None:intergenic 55.0%
GAGGTCCGATTATCTCGACC+TGG + chr5.3:65624172-65624191 MS.gene058508:CDS 55.0%
GCCTCATCAATCACCTGAGC+TGG - chr5.3:65624121-65624140 None:intergenic 55.0%
GGTGATTGATGAGGCGGTGT+TGG + chr5.3:65624126-65624145 MS.gene058508:CDS 55.0%
TCGGGAATAGGAACTGGACG+AGG - chr5.3:65625438-65625457 None:intergenic 55.0%
TGCTGGTGCGATGATGAGTG+TGG + chr5.3:65625814-65625833 MS.gene058508:CDS 55.0%
! CCAGTGTCGGGAATAGGAAC+TGG - chr5.3:65625444-65625463 None:intergenic 55.0%
ACCACAACCGCAACCAGCTC+AGG + chr5.3:65624105-65624124 MS.gene058508:CDS 60.0%
ACCTGAGCTGGTTGCGGTTG+TGG - chr5.3:65624109-65624128 None:intergenic 60.0%
CATTATCCGGCGACTCACGG+AGG + chr5.3:65624153-65624172 MS.gene058508:CDS 60.0%
TGAGCTGGTTGCGGTTGTGG+TGG - chr5.3:65624106-65624125 None:intergenic 60.0%
ATCGGACCTCCGTGAGTCGC+CGG - chr5.3:65624162-65624181 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 65624085 65625916 65624085 ID=MS.gene058508
chr5.3 mRNA 65624085 65625916 65624085 ID=MS.gene058508.t1;Parent=MS.gene058508
chr5.3 exon 65624085 65624307 65624085 ID=MS.gene058508.t1.exon1;Parent=MS.gene058508.t1
chr5.3 CDS 65624085 65624307 65624085 ID=cds.MS.gene058508.t1;Parent=MS.gene058508.t1
chr5.3 exon 65625055 65625614 65625055 ID=MS.gene058508.t1.exon2;Parent=MS.gene058508.t1
chr5.3 CDS 65625055 65625614 65625055 ID=cds.MS.gene058508.t1;Parent=MS.gene058508.t1
chr5.3 exon 65625728 65625916 65625728 ID=MS.gene058508.t1.exon3;Parent=MS.gene058508.t1
chr5.3 CDS 65625728 65625916 65625728 ID=cds.MS.gene058508.t1;Parent=MS.gene058508.t1
Gene Sequence

>MS.gene058508

ATGAGTGCACAAGGACAACCACCACAACCGCAACCAGCTCAGGTGATTGATGAGGCGGTGTTGGATGACATTATCCGGCGACTCACGGAGGTCCGATTATCTCGACCTGGAAAGCAAGTTCAGTTATCTGAAGCTGAGATCAAGCAGCTTTGTTCTGCTTCTAGAGATATCTTTCTTCAACAACCTAATTTGCTTGAACTTGAAGCTCCTATTAAGATTTGTGGTATATTTTTATGCTTACCCTTTTTTGACACCCCATTTTGATTTTTTTGTTTTTAGGGTTTAGAATGGCTTTTTTTAGTTGTTTGGTGATTTTGGGTGTGCTTAATTTGTTGCAAGTTTGAAGTTTTTGGTTTTAGGGTTTTCTCTTTTTACGAAGGTTTTAGGGTATGGTAGGGATTGAAAAAGAAAATGTGGATTTTGTTTTAATGTTCACGCTTTTGGGTATGCTTGATTTGAAGCTAAGAATGGAACTTTTAATTTTAGTGTGTATTTGATTTGATTGTTAGGTATTGAAGAAGAAAATGCAGTTTAAAATACATTTTTTAAATATTCATGTTATTGGGTATCCTTAATTTTTTGGGTACCTTTTTTTATCTTTTATTTTACAGCCACCCCAATTTGATAATTTTTATTACTAGGGTTTAAATTACTTTTCTTTTTAATGTTGTATTTGGATGTGTTTAATTTGTTGCAAGTTAGAACCTTTTGAATTTTAGGGTATATTTGGTTGTTTGGTAAGAATTGAATAATAAAATGTGGGTTACCCTTTTAGTTTTCAGAAAGTTTGGAGGGTTGTGAGAAAAACTATGTTTATGAACTTTTCTGCAAGTGTTTATTGGTTTTGCTTTGTCTTTAGACTTGTTGATTGCTGTTAAGGTTATTTTTAAGTGTGTGTTGTGGTCGAATTGAATGTACTATTTTATTAGTCTGGTATGGTTAAAATTGAAAATCCTGTTTGAATTTTCAGGTGACATTCATGGGCAGTACAGTGATTTGTTAAGGCTCTTTGAGTATGGGGGTTTGCCTCCTCAGTCTAATTATCTCTTTTTAGGGGACTATGTCGATCGTGGGAAGCAGAGCTTAGAAACCATTTGTCTATTGCTTGCTTATAAAATCAAATATCCTGAAAACTTTTTCCTTTTGAGGGGAAATCACGAGTGCGCTTCAATTAATAGGATATATGGCTTTTATGATGAATGTAAGCGAAGGTTTAACGTGAGGCTTTGGAAAGCCTTTACAGAATCTTTTAACTGCCTTCCTGTGGCAGCTCTTATAGATGAGAAAATATTGTGCATGCATGGTGGTCTTTCCCCTGACCTCACAAATTTGGACCAAATCCGGAATCTGCCTCGTCCAGTTCCTATTCCCGACACTGGTTTGCTCTGTGATTTGCTTTGGTCTGATCCTGGTAAAGATGTGAAGGGTTGGGGCATGAATGATAGAGGAGTGTCCTACACTTTTGGCCCTGATAAGGTGGCTGAATTTTTGACAAGGCATGACTTGGACCTTATTTGTCGTGCTCATCAGGTCACTATGTTTTTTCGCTCCTCCCTCCTCCACGTCATTTCTTTTACCTTCTTTGAGAAAAAGGCTAAACTTGTGTTGCTTTAATGGTAACTGATACTGTAATCTTTCTACAGGTTGTAGAGGATGGGTATGAATTCTTTGCAGACAGGCAACTTGTTACGATATTTTCAGCTCCAAACTACTGCGGTGAATTTGACAATGCTGGTGCGATGATGAGTGTGGACGAGAATTTAATGTGCTCCTTCCAGATTCTTAAGCCTGCAGAGAAGAAAACAAAGTTTGTGATGTCAAACAAGATGTGA
Protein sequence

>MS.gene058508.t1

MSAQGQPPQPQPAQVIDEAVLDDIIRRLTEVRLSRPGKQVQLSEAEIKQLCSASRDIFLQQPNLLELEAPIKICGDIHGQYSDLLRLFEYGGLPPQSNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNVRLWKAFTESFNCLPVAALIDEKILCMHGGLSPDLTNLDQIRNLPRPVPIPDTGLLCDLLWSDPGKDVKGWGMNDRGVSYTFGPDKVAEFLTRHDLDLICRAHQVVEDGYEFFADRQLVTIFSAPNYCGEFDNAGAMMSVDENLMCSFQILKPAEKKTKFVMSNKM