AlfalfaGEDB | MS.gene060689

M. sativa cultivar XinJiangDaYe / MS.gene060689

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF13041.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060689.t1	RHN60039.1	95	60	3	0	1	60	417	476	1.10E-22	115.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060689.t1	A0A396I342	95.0	60	3	0	1	60	417	476	7.8e-23	115.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052519	MS.gene060689	0.804667	1.94E-49	-1.69E-46
MS.gene052863	MS.gene060689	0.813992	1.93E-51	-1.69E-46
MS.gene055466	MS.gene060689	0.807003	6.25E-50	-1.69E-46
MS.gene058625	MS.gene060689	0.809249	2.08E-50	-1.69E-46
MS.gene060322	MS.gene060689	0.805747	1.15E-49	-1.69E-46
MS.gene060689	MS.gene060690	0.817148	3.83E-52	-1.69E-46
MS.gene060689	MS.gene061872	0.801582	8.41E-49	-1.69E-46
MS.gene060689	MS.gene061980	0.801808	7.56E-49	-1.69E-46
MS.gene060689	MS.gene062743	0.81097	8.85E-51	-1.69E-46
MS.gene060689	MS.gene066105	0.817185	3.76E-52	-1.69E-46
MS.gene060689	MS.gene068777	0.814175	1.76E-51	-1.69E-46
MS.gene060689	MS.gene08853	0.801082	1.06E-48	-1.69E-46
MS.gene060689	MS.gene20253	0.801185	1.01E-48	-1.69E-46
MS.gene060689	MS.gene29095	0.806396	8.39E-50	-1.69E-46
MS.gene060689	MS.gene38494	0.805608	1.23E-49	-1.69E-46
MS.gene060689	MS.gene40565	0.810334	1.21E-50	-1.69E-46
MS.gene060689	MS.gene45128	-0.824886	6.33E-54	-1.69E-46
MS.gene060689	MS.gene49283	0.820884	5.42E-53	-1.69E-46
MS.gene060689	MS.gene50250	0.802198	6.29E-49	-1.69E-46
MS.gene060689	MS.gene51511	0.816534	5.26E-52	-1.69E-46
MS.gene060689	MS.gene51788	0.818064	2.38E-52	-1.69E-46
MS.gene060689	MS.gene51818	0.805273	1.45E-49	-1.69E-46
MS.gene060689	MS.gene57178	0.801764	7.72E-49	-1.69E-46
MS.gene060689	MS.gene58608	-0.808515	2.98E-50	-1.69E-46
MS.gene060689	MS.gene64249	0.805262	1.45E-49	-1.69E-46
MS.gene060689	MS.gene65271	0.813795	2.14E-51	-1.69E-46
MS.gene060689	MS.gene67746	0.811481	6.85E-51	-1.69E-46
MS.gene060689	MS.gene71248	0.874278	7.65E-68	-1.69E-46
MS.gene060689	MS.gene71874	0.826963	2.03E-54	-1.69E-46
MS.gene060689	MS.gene81256	0.816555	5.21E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060689.t1	MTR_2g060630	93.333	60	4	0	1	60	438	497	7.80e-33	117

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCACTAGTTTCTCAGTTAT+TGG	0.185894	2.1:+21862427	None:intergenic
ATTCATGGACACTATGAAAA+TGG	0.201782	2.1:-21862511	MS.gene060689:CDS
CTCCTTCAACTGACCGTTCA+TGG	0.241734	2.1:+21862402	None:intergenic
AGGATGCCATGCGTTACTTC+CGG	0.351196	2.1:-21862480	MS.gene060689:CDS
GGATGCCATGCGTTACTTCC+GGG	0.379263	2.1:-21862479	MS.gene060689:CDS
TCGTATACCATAATGATTCA+TGG	0.404759	2.1:-21862526	None:intergenic
TTCCGGGAGATGACGTCTAA+AGG	0.461988	2.1:-21862463	MS.gene060689:CDS
TTCCATGAACGGTCAGTTGA+AGG	0.478957	2.1:-21862404	MS.gene060689:CDS
TCCGGGAGATGACGTCTAAA+GGG	0.496057	2.1:-21862462	MS.gene060689:CDS
TTCATGGACACTATGAAAAT+GGG	0.499216	2.1:-21862510	MS.gene060689:CDS
AGTTTCTCAGTTATTGGCTC+CGG	0.514899	2.1:+21862433	None:intergenic
TCCCTTTAGACGTCATCTCC+CGG	0.519194	2.1:+21862461	None:intergenic
GTAGAGGCATCTAATGTTTG+AGG	0.568426	2.1:-21862352	None:intergenic
ACAAGAAGGAGTTGAAGTAG+AGG	0.581060	2.1:-21862368	MS.gene060689:CDS
GACGTCTAAAGGGATAGTGC+CGG	0.603758	2.1:-21862452	MS.gene060689:CDS
CTATGAAAATGGGAGGACAA+AGG	0.642415	2.1:-21862500	MS.gene060689:CDS
CATCTCCCGGAAGTAACGCA+TGG	0.648521	2.1:+21862474	None:intergenic
AAACTAGTGATTTCCATGAA+CGG	0.662348	2.1:-21862415	MS.gene060689:CDS
ATGGACACTATGAAAATGGG+AGG	0.675142	2.1:-21862507	MS.gene060689:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACTAGTGATTTCCATGAA+CGG	-	chr2.1:21862453-21862472	MS.gene060689:CDS	30.0%
	ATTCATGGACACTATGAAAA+TGG	-	chr2.1:21862357-21862376	MS.gene060689:CDS	30.0%
	TTCATGGACACTATGAAAAT+GGG	-	chr2.1:21862358-21862377	MS.gene060689:CDS	30.0%
!	ATCACTAGTTTCTCAGTTAT+TGG	+	chr2.1:21862444-21862463	None:intergenic	30.0%
	GAGAGAACAGAAAAACAAGA+AGG	-	chr2.1:21862486-21862505	MS.gene060689:CDS	35.0%
	ACAAGAAGGAGTTGAAGTAG+AGG	-	chr2.1:21862500-21862519	MS.gene060689:CDS	40.0%
	ATGGACACTATGAAAATGGG+AGG	-	chr2.1:21862361-21862380	MS.gene060689:CDS	40.0%
	CTATGAAAATGGGAGGACAA+AGG	-	chr2.1:21862368-21862387	MS.gene060689:CDS	40.0%
!!	AGTTTCTCAGTTATTGGCTC+CGG	+	chr2.1:21862438-21862457	None:intergenic	40.0%
	TTCCATGAACGGTCAGTTGA+AGG	-	chr2.1:21862464-21862483	MS.gene060689:CDS	45.0%
	AGGATGCCATGCGTTACTTC+CGG	-	chr2.1:21862388-21862407	MS.gene060689:CDS	50.0%
	CTCCTTCAACTGACCGTTCA+TGG	+	chr2.1:21862469-21862488	None:intergenic	50.0%
	GACGTCTAAAGGGATAGTGC+CGG	-	chr2.1:21862416-21862435	MS.gene060689:CDS	50.0%
	TCCCTTTAGACGTCATCTCC+CGG	+	chr2.1:21862410-21862429	None:intergenic	50.0%
	TCCGGGAGATGACGTCTAAA+GGG	-	chr2.1:21862406-21862425	MS.gene060689:CDS	50.0%
	TTCCGGGAGATGACGTCTAA+AGG	-	chr2.1:21862405-21862424	MS.gene060689:CDS	50.0%
	CATCTCCCGGAAGTAACGCA+TGG	+	chr2.1:21862397-21862416	None:intergenic	55.0%
	GGATGCCATGCGTTACTTCC+GGG	-	chr2.1:21862389-21862408	MS.gene060689:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	21862354	21862536	21862354	ID=MS.gene060689
chr2.1	mRNA	21862354	21862536	21862354	ID=MS.gene060689.t1;Parent=MS.gene060689
chr2.1	exon	21862354	21862536	21862354	ID=MS.gene060689.t1.exon1;Parent=MS.gene060689.t1
chr2.1	CDS	21862354	21862536	21862354	ID=cds.MS.gene060689.t1;Parent=MS.gene060689.t1

Gene Sequence	>MS.gene060689 ATGATTCATGGACACTATGAAAATGGGAGGACAAAGGATGCCATGCGTTACTTCCGGGAGATGACGTCTAAAGGGATAGTGCCGGAGCCAATAACTGAGAAACTAGTGATTTCCATGAACGGTCAGTTGAAGGAGAGAACAGAAAAACAAGAAGGAGTTGAAGTAGAGGCATCTAATGTTTGA
Protein sequence	>MS.gene060689.t1 MIHGHYENGRTKDAMRYFREMTSKGIVPEPITEKLVISMNGQLKERTEKQEGVEVEASNV