AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58608


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACTTTGTCTAAATATTC+AGG 0.203271 5.3:+24449901 MS.gene58608:CDS
CTCGAGGTCGTCGGGGATTG+GGG 0.228861 5.3:-24449358 None:intergenic
ACTCGAGGTCGTCGGGGATT+GGG 0.272803 5.3:-24449359 None:intergenic
AATTAAGGTCTCTGAAAGTT+TGG 0.284932 5.3:-24449881 None:intergenic
GTCGTCGTAGCGTGCGTTCA+TGG 0.308557 5.3:+24449707 MS.gene58608:intron
AGTAGAGATATGGGATTTCT+TGG 0.308881 5.3:+24449327 None:intergenic
CTTTGTATGCATTGCATTGT+CGG 0.310634 5.3:-24450571 None:intergenic
ATATTTAGACAAAGTAATTA+AGG 0.354347 5.3:-24449896 None:intergenic
ATCGCTCTCGCCTTCTGCTT+TGG 0.354959 5.3:+24449423 MS.gene58608:CDS
AACTCGAGGTCGTCGGGGAT+TGG 0.372428 5.3:-24449360 None:intergenic
ATTCTTAATGCTGCACATAA+TGG 0.381497 5.3:+24449732 MS.gene58608:CDS
GGAGATAACTCGAGGTCGTC+GGG 0.416866 5.3:-24449366 None:intergenic
ATTAAGGTCTCTGAAAGTTT+GGG 0.424180 5.3:-24449880 None:intergenic
TCGAGGTCGTCGGGGATTGG+GGG 0.444858 5.3:-24449357 None:intergenic
ATGGCACTACCGATCCTTCT+CGG 0.445701 5.3:+24449751 MS.gene58608:CDS
AACCTACACCGGCTTTAAAC+CGG 0.447734 5.3:+24450059 MS.gene58608:CDS
TTACTTTGTCTAAATATTCA+GGG 0.448785 5.3:+24449902 MS.gene58608:CDS
ACATTATTTGTTACCCGAGA+AGG 0.497121 5.3:-24449765 None:intergenic
TGGCACTACCGATCCTTCTC+GGG 0.498527 5.3:+24449752 MS.gene58608:CDS
AAGCAGAAGGCGAGAGCGAT+CGG 0.498878 5.3:-24449420 None:intergenic
CTCGCCTTCTGCTTTGGTGT+AGG 0.509998 5.3:+24449429 MS.gene58608:CDS
CAATGCAATGCATACAAAGA+AGG 0.516235 5.3:+24450575 MS.gene58608:CDS
CTTGACTTCTTTGTACCATA+CGG 0.516923 5.3:-24449557 None:intergenic
TAGGTCATGTTACCTCCGTA+TGG 0.521416 5.3:+24449542 MS.gene58608:intron
CGAGGTCGTCGGGGATTGGG+GGG 0.522647 5.3:-24449356 None:intergenic
CTGATGAAGCGAATTTGAGC+GGG 0.536765 5.3:-24449387 None:intergenic
GGGAGATAACTCGAGGTCGT+CGG 0.564364 5.3:-24449367 None:intergenic
AAAGCTGACCGGTTTAAAGC+CGG 0.568877 5.3:-24450067 None:intergenic
GACTCGGACTCGAGATCTCA+AGG 0.575497 5.3:+24449924 MS.gene58608:CDS
AATGCAATGCATACAAAGAA+GGG 0.578153 5.3:+24450576 MS.gene58608:CDS
GGTGTAGGTTTCTCGAATGT+AGG 0.579764 5.3:-24450046 None:intergenic
ATTTGTTACCCGAGAAGGAT+CGG 0.588538 5.3:-24449760 None:intergenic
CCTTAAAATCGTAAACGACA+TGG 0.601425 5.3:-24449585 None:intergenic
GACCGGTTTAAAGCCGGTGT+AGG 0.604444 5.3:-24450061 None:intergenic
ACATCCTACACCAAAGCAGA+AGG 0.604708 5.3:-24449433 None:intergenic
AAAGAGAGAATAAAGCTGAC+CGG 0.607632 5.3:-24450078 None:intergenic
GACTTCTTTGTACCATACGG+AGG 0.618984 5.3:-24449554 None:intergenic
TGTCTAAATATTCAGGGACT+CGG 0.623538 5.3:+24449908 MS.gene58608:CDS
TCTGATGAAGCGAATTTGAG+CGG 0.624893 5.3:-24449388 None:intergenic
TACATTCGAGAAACCTACAC+CGG 0.663662 5.3:+24450048 MS.gene58608:CDS
GAGATAACTCGAGGTCGTCG+GGG 0.698069 5.3:-24449365 None:intergenic
TTTGAGCGGGAGATAACTCG+AGG 0.703118 5.3:-24449374 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAGACAAAGTAATTA+AGG - chr5.3:24449899-24449918 None:intergenic 15.0%
!! ATTCAAATTACTAACTAATT+AGG - chr5.3:24449966-24449985 None:intergenic 15.0%
!! AGATATTGTGTTAGAAAATA+TGG - chr5.3:24450543-24450562 None:intergenic 20.0%
!! ATTACTTTGTCTAAATATTC+AGG + chr5.3:24449901-24449920 MS.gene58608:CDS 20.0%
!! TGTTTGTTTGTTTGTATATT+TGG + chr5.3:24449685-24449704 MS.gene58608:intron 20.0%
!! TTACTTTGTCTAAATATTCA+GGG + chr5.3:24449902-24449921 MS.gene58608:CDS 20.0%
!!! AATATCAATTGATGATACAA+AGG - chr5.3:24449815-24449834 None:intergenic 20.0%
!!! ATATCAATTGATGATACAAA+GGG - chr5.3:24449814-24449833 None:intergenic 20.0%
!!! ATCATGCAATAGAATTTTTT+AGG + chr5.3:24450429-24450448 MS.gene58608:intron 20.0%
!!! TCAAATTGAAGTTTTAAATC+TGG - chr5.3:24450481-24450500 None:intergenic 20.0%
! GAAAAAGAATGAAAATGGAT+TGG - chr5.3:24449637-24449656 None:intergenic 25.0%
! GATAAATTACAATAGAACGA+TGG + chr5.3:24450250-24450269 MS.gene58608:intron 25.0%
! TACTAAATAACATACTGCTA+GGG + chr5.3:24450277-24450296 MS.gene58608:intron 25.0%
! TAGAGGAAAAAGAATGAAAA+TGG - chr5.3:24449642-24449661 None:intergenic 25.0%
! TCGTTCTATTGTAATTTATC+AGG - chr5.3:24450250-24450269 None:intergenic 25.0%
!! ATTTGAATCTTTTCCTCTAT+CGG + chr5.3:24450495-24450514 MS.gene58608:intron 25.0%
!! TATCAATTGATGATACAAAG+GGG - chr5.3:24449813-24449832 None:intergenic 25.0%
!!! GGTTTAATTGTTTCTCTTTT+CGG + chr5.3:24450298-24450317 MS.gene58608:intron 25.0%
!!! TGAAGTTTTAAATCTGGATA+GGG - chr5.3:24450475-24450494 None:intergenic 25.0%
!!! TTGAAGTTTTAAATCTGGAT+AGG - chr5.3:24450476-24450495 None:intergenic 25.0%
AAATCCTCCATAAAACACTA+TGG + chr5.3:24450151-24450170 MS.gene58608:intron 30.0%
AACATGACCTAAAAGGAAAA+AGG - chr5.3:24449533-24449552 None:intergenic 30.0%
AATGCAATGCATACAAAGAA+GGG + chr5.3:24450576-24450595 MS.gene58608:CDS 30.0%
ATTCTTAATGCTGCACATAA+TGG + chr5.3:24449732-24449751 MS.gene58608:CDS 30.0%
CACTCGCAAAAGAAAATAAT+TGG + chr5.3:24450185-24450204 MS.gene58608:intron 30.0%
GTACTAAATAACATACTGCT+AGG + chr5.3:24450276-24450295 MS.gene58608:intron 30.0%
GTTAGTTAGTTAGTTAGTAG+AGG - chr5.3:24449659-24449678 None:intergenic 30.0%
! AATTAAGGTCTCTGAAAGTT+TGG - chr5.3:24449884-24449903 None:intergenic 30.0%
! ATTAAGGTCTCTGAAAGTTT+GGG - chr5.3:24449883-24449902 None:intergenic 30.0%
! TAGTGTTTTATGGAGGATTT+TGG - chr5.3:24450151-24450170 None:intergenic 30.0%
! TCTCATTCCTTTTTCCTTTT+AGG + chr5.3:24449523-24449542 MS.gene58608:intron 30.0%
! TTTGAATCTTTTCCTCTATC+GGG + chr5.3:24450496-24450515 MS.gene58608:intron 30.0%
!! ATTTTGGCAGTTTTATACCA+TGG - chr5.3:24450135-24450154 None:intergenic 30.0%
!!! GAAGTTTTAAATCTGGATAG+GGG - chr5.3:24450474-24450493 None:intergenic 30.0%
AAAGAGAGAATAAAGCTGAC+CGG - chr5.3:24450081-24450100 None:intergenic 35.0%
ATTTATACAGATCCCGATAG+AGG - chr5.3:24450511-24450530 None:intergenic 35.0%
CAATGCAATGCATACAAAGA+AGG + chr5.3:24450575-24450594 MS.gene58608:CDS 35.0%
CCTTAAAATCGTAAACGACA+TGG - chr5.3:24449588-24449607 None:intergenic 35.0%
CGGGATCTGTATAAATTCTA+GGG + chr5.3:24450515-24450534 MS.gene58608:intron 35.0%
CGTGTTAAGAGAATCAACAA+GGG - chr5.3:24450327-24450346 None:intergenic 35.0%
CTTGACTTCTTTGTACCATA+CGG - chr5.3:24449560-24449579 None:intergenic 35.0%
CTTTGTATGCATTGCATTGT+CGG - chr5.3:24450574-24450593 None:intergenic 35.0%
TCGGGATCTGTATAAATTCT+AGG + chr5.3:24450514-24450533 MS.gene58608:intron 35.0%
TGTCTAAATATTCAGGGACT+CGG + chr5.3:24449908-24449927 MS.gene58608:CDS 35.0%
! ACATTATTTGTTACCCGAGA+AGG - chr5.3:24449768-24449787 None:intergenic 35.0%
! ATTTTCTTTTGCGAGTGTGA+AGG - chr5.3:24450182-24450201 None:intergenic 35.0%
! TTTTCTTTTGCGAGTGTGAA+GGG - chr5.3:24450181-24450200 None:intergenic 35.0%
!! AATGCCTTTAAAACCCTGTA+GGG - chr5.3:24450019-24450038 None:intergenic 35.0%
!! CCATGTCGTTTACGATTTTA+AGG + chr5.3:24449585-24449604 MS.gene58608:CDS 35.0%
!! CCTTGTTGATTCTCTTAACA+CGG + chr5.3:24450325-24450344 MS.gene58608:intron 35.0%
CCGTGTTAAGAGAATCAACA+AGG - chr5.3:24450328-24450347 None:intergenic 40.0%
TACATTCGAGAAACCTACAC+CGG + chr5.3:24450048-24450067 MS.gene58608:CDS 40.0%
TCTGATGAAGCGAATTTGAG+CGG - chr5.3:24449391-24449410 None:intergenic 40.0%
TTATCGATCGATCGTTACCA+TGG + chr5.3:24450115-24450134 MS.gene58608:intron 40.0%
! ATTTGTTACCCGAGAAGGAT+CGG - chr5.3:24449763-24449782 None:intergenic 40.0%
! TTTCTTTTGCGAGTGTGAAG+GGG - chr5.3:24450180-24450199 None:intergenic 40.0%
!! ATGCCTTTAAAACCCTGTAG+GGG - chr5.3:24450018-24450037 None:intergenic 40.0%
!! CAATGCCTTTAAAACCCTGT+AGG - chr5.3:24450020-24450039 None:intergenic 40.0%
!!! CTACAGGGTTTTAAAGGCAT+TGG + chr5.3:24450018-24450037 MS.gene58608:intron 40.0%
ACATCCTACACCAAAGCAGA+AGG - chr5.3:24449436-24449455 None:intergenic 45.0%
CGGAGGTAACATGACCTAAA+AGG - chr5.3:24449540-24449559 None:intergenic 45.0%
CTGATGAAGCGAATTTGAGC+GGG - chr5.3:24449390-24449409 None:intergenic 45.0%
GACTTCTTTGTACCATACGG+AGG - chr5.3:24449557-24449576 None:intergenic 45.0%
GGTGTAGGTTTCTCGAATGT+AGG - chr5.3:24450049-24450068 None:intergenic 45.0%
TAGGTCATGTTACCTCCGTA+TGG + chr5.3:24449542-24449561 MS.gene58608:intron 45.0%
! AAAGCTGACCGGTTTAAAGC+CGG - chr5.3:24450070-24450089 None:intergenic 45.0%
! TTCTTTTGCGAGTGTGAAGG+GGG - chr5.3:24450179-24450198 None:intergenic 45.0%
!! AACCTACACCGGCTTTAAAC+CGG + chr5.3:24450059-24450078 MS.gene58608:CDS 45.0%
!! TGCCTTTAAAACCCTGTAGG+GGG - chr5.3:24450017-24450036 None:intergenic 45.0%
ATGGCACTACCGATCCTTCT+CGG + chr5.3:24449751-24449770 MS.gene58608:CDS 50.0%
TTTGAGCGGGAGATAACTCG+AGG - chr5.3:24449377-24449396 None:intergenic 50.0%
! ACTGTTTTCACCCCCCTACA+GGG + chr5.3:24450003-24450022 MS.gene58608:intron 50.0%
! GGCGCCATAGTGTTTTATGG+AGG - chr5.3:24450158-24450177 None:intergenic 50.0%
! TACTGTTTTCACCCCCCTAC+AGG + chr5.3:24450002-24450021 MS.gene58608:intron 50.0%
!! ACCCCCCTACAGGGTTTTAA+AGG + chr5.3:24450012-24450031 MS.gene58608:intron 50.0%
!! GCCTTTAAAACCCTGTAGGG+GGG - chr5.3:24450016-24450035 None:intergenic 50.0%
AAGCAGAAGGCGAGAGCGAT+CGG - chr5.3:24449423-24449442 None:intergenic 55.0%
ATCGCTCTCGCCTTCTGCTT+TGG + chr5.3:24449423-24449442 MS.gene58608:CDS 55.0%
GACTCGGACTCGAGATCTCA+AGG + chr5.3:24449924-24449943 MS.gene58608:CDS 55.0%
GAGATAACTCGAGGTCGTCG+GGG - chr5.3:24449368-24449387 None:intergenic 55.0%
GGAGATAACTCGAGGTCGTC+GGG - chr5.3:24449369-24449388 None:intergenic 55.0%
GGGAGATAACTCGAGGTCGT+CGG - chr5.3:24449370-24449389 None:intergenic 55.0%
TGGCACTACCGATCCTTCTC+GGG + chr5.3:24449752-24449771 MS.gene58608:CDS 55.0%
! CTCGCCTTCTGCTTTGGTGT+AGG + chr5.3:24449429-24449448 MS.gene58608:CDS 55.0%
! GACCGGTTTAAAGCCGGTGT+AGG - chr5.3:24450064-24450083 None:intergenic 55.0%
!!! GGGGGCGCCATAGTGTTTTA+TGG - chr5.3:24450161-24450180 None:intergenic 55.0%
AACTCGAGGTCGTCGGGGAT+TGG - chr5.3:24449363-24449382 None:intergenic 60.0%
ACTCGAGGTCGTCGGGGATT+GGG - chr5.3:24449362-24449381 None:intergenic 60.0%
GTCGTCGTAGCGTGCGTTCA+TGG + chr5.3:24449707-24449726 MS.gene58608:intron 60.0%
CTCGAGGTCGTCGGGGATTG+GGG - chr5.3:24449361-24449380 None:intergenic 65.0%
TCGAGGTCGTCGGGGATTGG+GGG - chr5.3:24449360-24449379 None:intergenic 65.0%
CGAGGTCGTCGGGGATTGGG+GGG - chr5.3:24449359-24449378 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 24449336 24450605 24449336 ID=MS.gene58608
chr5.3 mRNA 24449336 24450605 24449336 ID=MS.gene58608.t1;Parent=MS.gene58608
chr5.3 exon 24449336 24449453 24449336 ID=MS.gene58608.t1.exon1;Parent=MS.gene58608.t1
chr5.3 CDS 24449336 24449453 24449336 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
chr5.3 exon 24449545 24449606 24449545 ID=MS.gene58608.t1.exon2;Parent=MS.gene58608.t1
chr5.3 CDS 24449545 24449606 24449545 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
chr5.3 exon 24449717 24449773 24449717 ID=MS.gene58608.t1.exon3;Parent=MS.gene58608.t1
chr5.3 CDS 24449717 24449773 24449717 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
chr5.3 exon 24449871 24449945 24449871 ID=MS.gene58608.t1.exon4;Parent=MS.gene58608.t1
chr5.3 CDS 24449871 24449945 24449871 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
chr5.3 exon 24450024 24450107 24450024 ID=MS.gene58608.t1.exon5;Parent=MS.gene58608.t1
chr5.3 CDS 24450024 24450107 24450024 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
chr5.3 exon 24450570 24450605 24450570 ID=MS.gene58608.t1.exon6;Parent=MS.gene58608.t1
chr5.3 CDS 24450570 24450605 24450570 ID=cds.MS.gene58608.t1;Parent=MS.gene58608.t1
Gene Sequence

>MS.gene58608

ATGGGATTTCTTGGCTATGACCCCCCAATCCCCGACGACCTCGAGTTATCTCCCGCTCAAATTCGCTTCATCAGAATAACACATCCGATCGCTCTCGCCTTCTGCTTTGGTGTAGGATGTATTTTTCTATCTCTCTCTCTCTCTTTCTTCGTTGATTTTATTATTATTATTATTATGTTATTGCTCATCTCATTCCTTTTTCCTTTTAGGTCATGTTACCTCCGTATGGTACAAAGAAGTCAAGTTCGACCATGTCGTTTACGATTTTAAGGTAGTAATAATAATACAACTAACTTTTCCAATCCATTTTCATTCTTTTTCCTCTACTAACTAACTAACTAACTAACTCTGTTTGTTTGTTTGTATATTTGGTCGTCGTAGCGTGCGTTCATGGATATTCTTAATGCTGCACATAATGGCACTACCGATCCTTCTCGGGTAACAAATAATGTCTTTTATGTTTTTTCAAGTTATCCCCTTTGTATCATCAATTGATATTGATCTCATGTTAATTAATTTATTAATTAATATTCAGCTGAAGCGACCCAAACTTTCAGAGACCTTAATTACTTTGTCTAAATATTCAGGGACTCGGACTCGAGATCTCAAGGTAGATACTACTCTTTTCCTAATTAGTTAGTAATTTGAATGAAATTTATATGATACTACTGTTTTCACCCCCCTACAGGGTTTTAAAGGCATTGGAGATACCTACATTCGAGAAACCTACACCGGCTTTAAACCGGTCAGCTTTATTCTCTCTTTGCTCTATGTCAGTATTATCGATCGATCGTTACCATGGTATAAAACTGCCAAAATCCTCCATAAAACACTATGGCGCCCCCTTCACACTCGCAAAAGAAAATAATTGGAGTGAAAACAATGTAGACTATTAATGTCAGAAAGTAGTGCCTGATAAATTACAATAGAACGATGGCTTGTACTAAATAACATACTGCTAGGGTTTAATTGTTTCTCTTTTCGGAAACCCTTGTTGATTCTCTTAACACGGAAACATAATATCATAACTAGAACTAGACAGAATTCAAATTATTAAACATACATTTTAATAGAAGCAATTCAGCTGTGCACTATCATGCAATAGAATTTTTTAGGATCAGCTTCTATCTATACTCCCCTATCCAGATTTAAAACTTCAATTTGAATCTTTTCCTCTATCGGGATCTGTATAAATTCTAGGGCTCCATATTTTCTAACACAATATCTTTTGTAGACCGACAATGCAATGCATACAAAGAAGGGTATATGA

Protein sequence

>MS.gene58608.t1

MGFLGYDPPIPDDLELSPAQIRFIRITHPIALAFCFGVGCHVTSVWYKEVKFDHVVYDFKRAFMDILNAAHNGTTDPSRLKRPKLSETLITLSKYSGTRTRDLKGFKGIGDTYIRETYTGFKPVSFILSLLYTDNAMHTKKGI