AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81256


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTTCTATTGATACAAT+TGG 0.313466 5.2:-81344079 None:intergenic
TTGTAATGCTCTTCTTCTTA+AGG 0.320244 5.2:+81344829 MS.gene81256:CDS
ACAGGCAAAGGAAGCGCATT+GGG 0.339363 5.2:+81345365 MS.gene81256:CDS
ATCCATTTGACAAGGAAGAT+TGG 0.376004 5.2:+81344098 MS.gene81256:CDS
TGAATGATTCTGCAGGTAGT+AGG 0.386036 5.2:+81344749 MS.gene81256:intron
CTTTCACATTCTTATGCTGA+TGG 0.397629 5.2:+81345523 MS.gene81256:CDS
TCCATTTGACAAGGAAGATT+GGG 0.410260 5.2:+81344099 MS.gene81256:CDS
TCCCAATCTTCCTTGTCAAA+TGG 0.418960 5.2:-81344100 None:intergenic
TTCATAGCTGATTTATCCGT+TGG 0.433997 5.2:+81345429 MS.gene81256:CDS
GATCTTCTATTGATACAATT+GGG 0.456790 5.2:-81344078 None:intergenic
GGTCATTGAAGTGGTCAAAC+AGG 0.458747 5.2:+81345347 MS.gene81256:CDS
CTCAAGCCTTGGAATTTGCC+AGG 0.463723 5.2:+81344138 MS.gene81256:CDS
GAATGATTCTGCAGGTAGTA+GGG 0.464232 5.2:+81344750 MS.gene81256:intron
ATCAAGTATATCTCAAGCCT+TGG 0.478687 5.2:+81344127 MS.gene81256:CDS
CCTCGGTCACAGTTCCAACT+TGG 0.498921 5.2:-81345326 None:intergenic
GCTGTTGGTCAGATTAAAGC+AGG 0.505927 5.2:+81345456 MS.gene81256:CDS
TTATCCGTTGGCCTTGCTGT+TGG 0.541949 5.2:+81345441 MS.gene81256:CDS
AACAGGCAAAGGAAGCGCAT+TGG 0.543307 5.2:+81345364 MS.gene81256:CDS
GTGATCCAGGTCAACCAAGT+TGG 0.555500 5.2:+81345312 MS.gene81256:intron
TCTAATCGCTCTCCTCTGCA+AGG 0.555562 5.2:-81345483 None:intergenic
ATAATACCTGGCAAATTCCA+AGG 0.562772 5.2:-81344144 None:intergenic
AGGCAAAGGAAGCGCATTGG+GGG 0.571983 5.2:+81345367 MS.gene81256:CDS
TGAAGTGGTCAAACAGGCAA+AGG 0.579709 5.2:+81345353 MS.gene81256:CDS
CCAAGTTGGAACTGTGACCG+AGG 0.585510 5.2:+81345326 MS.gene81256:CDS
CAGGCAAAGGAAGCGCATTG+GGG 0.596386 5.2:+81345366 MS.gene81256:CDS
AATGATTCTGCAGGTAGTAG+GGG 0.598973 5.2:+81344751 MS.gene81256:intron
TTTGACCACTTCAATGACCT+CGG 0.604404 5.2:-81345343 None:intergenic
GTGGTGACATCTCACAGAAG+TGG 0.607193 5.2:+81345390 MS.gene81256:CDS
AAAGCAGGCGCTCCTTGCAG+AGG 0.636542 5.2:+81345471 MS.gene81256:CDS
TGTGACCGAGGTCATTGAAG+TGG 0.639474 5.2:+81345338 MS.gene81256:CDS
CTTTAATCTGACCAACAGCA+AGG 0.642755 5.2:-81345452 None:intergenic
GGCAAAGGAAGCGCATTGGG+GGG 0.654274 5.2:+81345368 MS.gene81256:CDS
ATAAGAATGTGAAAGAGACC+TGG 0.665974 5.2:-81345515 None:intergenic
CTGACCAACAGCAAGGCCAA+CGG 0.676271 5.2:-81345445 None:intergenic
AATAGAAGATCCATTTGACA+AGG 0.686135 5.2:+81344090 MS.gene81256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCATTTATATGATAAAAAA+TGG - chr5.2:81344949-81344968 None:intergenic 15.0%
!!! TTCTTTTATGCTATTATTAT+TGG + chr5.2:81344268-81344287 MS.gene81256:intron 15.0%
!!! TTGAAAAGTTAACATTTTAT+TGG + chr5.2:81344170-81344189 MS.gene81256:intron 15.0%
!! AATGAAAACTAATAACAAAC+AGG + chr5.2:81344561-81344580 MS.gene81256:intron 20.0%
!! ACACTTAAAAAAGAAAGATA+TGG + chr5.2:81344437-81344456 MS.gene81256:intron 20.0%
!! GTAACATTAAACTTTGAATT+TGG + chr5.2:81344379-81344398 MS.gene81256:intron 20.0%
!!! AATGCTAAATTTTTTGTTGA+TGG + chr5.2:81344963-81344982 MS.gene81256:intron 20.0%
!!! ACTTTTCAATAAATAATACC+TGG - chr5.2:81344159-81344178 None:intergenic 20.0%
!!! TTTTCTTCAACAAAATAGAA+GGG - chr5.2:81345263-81345282 None:intergenic 20.0%
!!! TTTTTAAGTGTCGATTTTTT+AGG - chr5.2:81344428-81344447 None:intergenic 20.0%
! AGAAAAGAAACATGAAAATG+TGG + chr5.2:81345277-81345296 MS.gene81256:intron 25.0%
! ATTAACATGTTAGGTAAGAA+AGG + chr5.2:81344613-81344632 MS.gene81256:intron 25.0%
! ATTGTTTCAATGAACTTTCA+GGG + chr5.2:81344861-81344880 MS.gene81256:intron 25.0%
! GATCTTCTATTGATACAATT+GGG - chr5.2:81344081-81344100 None:intergenic 25.0%
! TATTGTTTCAATGAACTTTC+AGG + chr5.2:81344860-81344879 MS.gene81256:intron 25.0%
! TGCAGAATCATTCAAATTAA+GGG - chr5.2:81344743-81344762 None:intergenic 25.0%
! TTAACATGTTAGGTAAGAAA+GGG + chr5.2:81344614-81344633 MS.gene81256:intron 25.0%
!! ATCTATTTGTTAGCTTACAT+TGG + chr5.2:81344658-81344677 MS.gene81256:intron 25.0%
!! CTTTTCTTCAACAAAATAGA+AGG - chr5.2:81345264-81345283 None:intergenic 25.0%
!! TCTATTTGTTAGCTTACATT+GGG + chr5.2:81344659-81344678 MS.gene81256:intron 25.0%
!!! GAATAAAAAGCATCAATCTT+TGG - chr5.2:81344501-81344520 None:intergenic 25.0%
AATAGAAGATCCATTTGACA+AGG + chr5.2:81344090-81344109 MS.gene81256:CDS 30.0%
ATATCTCATAACCTCCAAAA+CGG - chr5.2:81344538-81344557 None:intergenic 30.0%
CCTCAACTTAATTATGAAAC+AGG - chr5.2:81345002-81345021 None:intergenic 30.0%
CTATTGCAGAGACAAAAAAA+TGG - chr5.2:81344326-81344345 None:intergenic 30.0%
CTGCAGAATCATTCAAATTA+AGG - chr5.2:81344744-81344763 None:intergenic 30.0%
GAAAAAAAATGCAAACACAG+TGG - chr5.2:81345188-81345207 None:intergenic 30.0%
GGATCTTCTATTGATACAAT+TGG - chr5.2:81344082-81344101 None:intergenic 30.0%
TAACATGTTAGGTAAGAAAG+GGG + chr5.2:81344615-81344634 MS.gene81256:intron 30.0%
TGATGACACATTAACATGTT+AGG + chr5.2:81344604-81344623 MS.gene81256:intron 30.0%
TTCCTCTCTATTTATCTAGA+GGG + chr5.2:81345205-81345224 MS.gene81256:intron 30.0%
TTGTAATGCTCTTCTTCTTA+AGG + chr5.2:81344829-81344848 MS.gene81256:CDS 30.0%
TTGTCTCTGCAATAGTTAAA+GGG + chr5.2:81344331-81344350 MS.gene81256:intron 30.0%
TTTCCTCTCTATTTATCTAG+AGG + chr5.2:81345204-81345223 MS.gene81256:intron 30.0%
TTTGTCTCTGCAATAGTTAA+AGG + chr5.2:81344330-81344349 MS.gene81256:intron 30.0%
! CCTGTTTCATAATTAAGTTG+AGG + chr5.2:81344999-81345018 MS.gene81256:intron 30.0%
! CTTAATTTGAATGATTCTGC+AGG + chr5.2:81344742-81344761 MS.gene81256:intron 30.0%
! GTTAAGCAGCTATAATTTGT+TGG + chr5.2:81344467-81344486 MS.gene81256:intron 30.0%
!! CATAATTAAGTTGAGGCATT+GGG + chr5.2:81345006-81345025 MS.gene81256:intron 30.0%
!! TCATAATTAAGTTGAGGCAT+TGG + chr5.2:81345005-81345024 MS.gene81256:intron 30.0%
AATGGCGAAATGAAATGTCA+AGG - chr5.2:81344308-81344327 None:intergenic 35.0%
ACAGGTTATTAGTGTAGCAA+TGG + chr5.2:81344579-81344598 MS.gene81256:intron 35.0%
ACCTGGAAAGACTCTTAAAA+AGG - chr5.2:81344215-81344234 None:intergenic 35.0%
ATAAGAATGTGAAAGAGACC+TGG - chr5.2:81345518-81345537 None:intergenic 35.0%
ATAATACCTGGCAAATTCCA+AGG - chr5.2:81344147-81344166 None:intergenic 35.0%
ATCAAGTATATCTCAAGCCT+TGG + chr5.2:81344127-81344146 MS.gene81256:CDS 35.0%
ATCCATTTGACAAGGAAGAT+TGG + chr5.2:81344098-81344117 MS.gene81256:CDS 35.0%
ATGGCGAAATGAAATGTCAA+GGG - chr5.2:81344307-81344326 None:intergenic 35.0%
GCATTAGTAAGGTTGCTTAT+TGG + chr5.2:81344686-81344705 MS.gene81256:intron 35.0%
GCGATTAGAAAAATACAACC+AGG + chr5.2:81345497-81345516 MS.gene81256:CDS 35.0%
TCCATTTGACAAGGAAGATT+GGG + chr5.2:81344099-81344118 MS.gene81256:CDS 35.0%
! AGGTTTTTGTATGCTAACGA+CGG + chr5.2:81344231-81344250 MS.gene81256:intron 35.0%
! GTATCTTCAAACTCTGGAAA+AGG + chr5.2:81345028-81345047 MS.gene81256:intron 35.0%
! TCTCTCAGTTTGTTTAAGCT+TGG + chr5.2:81344916-81344935 MS.gene81256:intron 35.0%
! TGTAGTAAGCATTACCGTTT+TGG + chr5.2:81344521-81344540 MS.gene81256:intron 35.0%
! TTCATAGCTGATTTATCCGT+TGG + chr5.2:81345429-81345448 MS.gene81256:CDS 35.0%
!! TCCTTTTTAAGAGTCTTTCC+AGG + chr5.2:81344211-81344230 MS.gene81256:intron 35.0%
AATGATTCTGCAGGTAGTAG+GGG + chr5.2:81344751-81344770 MS.gene81256:intron 40.0%
CAGTGGCAAAACTAAAGTAC+TGG - chr5.2:81345171-81345190 None:intergenic 40.0%
CATTGGGTATCTTCAAACTC+TGG + chr5.2:81345022-81345041 MS.gene81256:intron 40.0%
CTTTAATCTGACCAACAGCA+AGG - chr5.2:81345455-81345474 None:intergenic 40.0%
GAATGATTCTGCAGGTAGTA+GGG + chr5.2:81344750-81344769 MS.gene81256:intron 40.0%
GTCGTTAGCATACAAAAACC+TGG - chr5.2:81344232-81344251 None:intergenic 40.0%
TCCCAATCTTCCTTGTCAAA+TGG - chr5.2:81344103-81344122 None:intergenic 40.0%
TGAATGATTCTGCAGGTAGT+AGG + chr5.2:81344749-81344768 MS.gene81256:intron 40.0%
TGCCCTCTAGATAAATAGAG+AGG - chr5.2:81345210-81345229 None:intergenic 40.0%
TTTGACCACTTCAATGACCT+CGG - chr5.2:81345346-81345365 None:intergenic 40.0%
! AGTAAGCATTACCGTTTTGG+AGG + chr5.2:81344524-81344543 MS.gene81256:intron 40.0%
! ATAGAAGGGAAGTACATGAG+AGG - chr5.2:81345249-81345268 None:intergenic 40.0%
! CTCAGTTTGTTTAAGCTTGG+AGG + chr5.2:81344919-81344938 MS.gene81256:intron 40.0%
! GTTATTTCCCATTGTGATCC+AGG + chr5.2:81345299-81345318 MS.gene81256:intron 40.0%
!! CATTGGGTTGAGCATTAGTA+AGG + chr5.2:81344675-81344694 MS.gene81256:intron 40.0%
!! GCAGCTATAATTTGTTGGCT+AGG + chr5.2:81344472-81344491 MS.gene81256:intron 40.0%
GCTGTTGGTCAGATTAAAGC+AGG + chr5.2:81345456-81345475 MS.gene81256:CDS 45.0%
GGTCATTGAAGTGGTCAAAC+AGG + chr5.2:81345347-81345366 MS.gene81256:CDS 45.0%
TGAAGTGGTCAAACAGGCAA+AGG + chr5.2:81345353-81345372 MS.gene81256:CDS 45.0%
TGGTTGACCTGGATCACAAT+GGG - chr5.2:81345309-81345328 None:intergenic 45.0%
TTGGTTGACCTGGATCACAA+TGG - chr5.2:81345310-81345329 None:intergenic 45.0%
CAGTTCCAACTTGGTTGACC+TGG - chr5.2:81345320-81345339 None:intergenic 50.0%
CTCAAGCCTTGGAATTTGCC+AGG + chr5.2:81344138-81344157 MS.gene81256:CDS 50.0%
GTGATCCAGGTCAACCAAGT+TGG + chr5.2:81345312-81345331 MS.gene81256:intron 50.0%
GTGGTGACATCTCACAGAAG+TGG + chr5.2:81345390-81345409 MS.gene81256:CDS 50.0%
TCTAATCGCTCTCCTCTGCA+AGG - chr5.2:81345486-81345505 None:intergenic 50.0%
TGTGACCGAGGTCATTGAAG+TGG + chr5.2:81345338-81345357 MS.gene81256:CDS 50.0%
! AACAGGCAAAGGAAGCGCAT+TGG + chr5.2:81345364-81345383 MS.gene81256:CDS 50.0%
! ACAGGCAAAGGAAGCGCATT+GGG + chr5.2:81345365-81345384 MS.gene81256:CDS 50.0%
! GAAGGGAAGTACATGAGAGG+AGG - chr5.2:81345246-81345265 None:intergenic 50.0%
!! TTATCCGTTGGCCTTGCTGT+TGG + chr5.2:81345441-81345460 MS.gene81256:CDS 50.0%
CCTCGGTCACAGTTCCAACT+TGG - chr5.2:81345329-81345348 None:intergenic 55.0%
CTGACCAACAGCAAGGCCAA+CGG - chr5.2:81345448-81345467 None:intergenic 55.0%
! AGGCAAAGGAAGCGCATTGG+GGG + chr5.2:81345367-81345386 MS.gene81256:CDS 55.0%
! CAGGCAAAGGAAGCGCATTG+GGG + chr5.2:81345366-81345385 MS.gene81256:CDS 55.0%
! CCAAGTTGGAACTGTGACCG+AGG + chr5.2:81345326-81345345 MS.gene81256:CDS 55.0%
AAAGCAGGCGCTCCTTGCAG+AGG + chr5.2:81345471-81345490 MS.gene81256:CDS 60.0%
! AAAGGAAGCGCATTGGGGGG+TGG + chr5.2:81345371-81345390 MS.gene81256:CDS 60.0%
! GGCAAAGGAAGCGCATTGGG+GGG + chr5.2:81345368-81345387 MS.gene81256:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81344074 81345542 81344074 ID=MS.gene81256
chr5.2 mRNA 81344074 81345542 81344074 ID=MS.gene81256.t1;Parent=MS.gene81256
chr5.2 exon 81344074 81344159 81344074 ID=MS.gene81256.t1.exon1;Parent=MS.gene81256.t1
chr5.2 CDS 81344074 81344159 81344074 ID=cds.MS.gene81256.t1;Parent=MS.gene81256.t1
chr5.2 exon 81344764 81344850 81344764 ID=MS.gene81256.t1.exon2;Parent=MS.gene81256.t1
chr5.2 CDS 81344764 81344850 81344764 ID=cds.MS.gene81256.t1;Parent=MS.gene81256.t1
chr5.2 exon 81345321 81345542 81345321 ID=MS.gene81256.t1.exon3;Parent=MS.gene81256.t1
chr5.2 CDS 81345321 81345542 81345321 ID=cds.MS.gene81256.t1;Parent=MS.gene81256.t1
Gene Sequence

>MS.gene81256

AATACCCAATTGTATCAATAGAAGATCCATTTGACAAGGAAGATTGGGAACATATCAAGTATATCTCAAGCCTTGGAATTTGCCAGGTATTATTTATTGAAAAGTTAACATTTTATTGGAATTAGTCAAGTTTCATTTCCTTTTTAAGAGTCTTTCCAGGTTTTTGTATGCTAACGACGGATATCTTGTGTTTTTTCTTTTATGCTATTATTATTGGTCTTTTTGTTATACCCTTGACATTTCATTTCGCCATTTTTTTGTCTCTGCAATAGTTAAAGGGTACAATTGACAAAATAATAATTAATGTAACATTAAACTTTGAATTTGGCAAGTAAATAATAATATTTTTTTCCTAAAAAATCGACACTTAAAAAAGAAAGATATGGAGTTTCTGTTAAGCAGCTATAATTTGTTGGCTAGGCATCCAAAGATTGATGCTTTTTATTCTGTAGTAAGCATTACCGTTTTGGAGGTTATGAGATATTTTAATGAAAACTAATAACAAACAGGTTATTAGTGTAGCAATGGCATGATGACACATTAACATGTTAGGTAAGAAAGGGGTGAAAAAAAAGTAATAAATAATCTATTTGTTAGCTTACATTGGGTTGAGCATTAGTAAGGTTGCTTATTGGATACTGATTAGAACTTCTGTTGATATAATTTCCCTTAATTTGAATGATTCTGCAGGTAGTAGGGGATGATCTCTTGATGTCTAATGCAAAGCGCATTGAGAGAGCAGTGACTGAGTCTGCTTGTAATGCTCTTCTTCTTAAGGTAACTGAATATTGTTTCAATGAACTTTCAGGGCGTTGCTTTGTCTAATAATAAATTTAATTTTATCTCTCAGTTTGTTTAAGCTTGGAGGTTTTCCATTTTTTATCATATAAATGCTAAATTTTTTGTTGATGGAATGATAACTAATCCTGTTTCATAATTAAGTTGAGGCATTGGGTATCTTCAAACTCTGGAAAAGGTTAATTTTTTGTGCAAATACGAGATCACAATTTACAAAGCTAGTTTTTATGAAAGAGAAAAATATAAGATCACAATTTACAAATCTAGTTTAGTTTATATGAAAGAGAAAAAGATTGCCAGTACTTTAGTTTTGCCACTGTGTTTGCATTTTTTTTCCTCTCTATTTATCTAGAGGGCATTTTAATTTTAATCCTCCTCTCATGTACTTCCCTTCTATTTTGTTGAAGAAAAGAAACATGAAAATGTGGTTATTTCCCATTGTGATCCAGGTCAACCAAGTTGGAACTGTGACCGAGGTCATTGAAGTGGTCAAACAGGCAAAGGAAGCGCATTGGGGGGTGGTGACATCTCACAGAAGTGGAGAAACTGTAGACTCTTTCATAGCTGATTTATCCGTTGGCCTTGCTGTTGGTCAGATTAAAGCAGGCGCTCCTTGCAGAGGAGAGCGATTAGAAAAATACAACCAGGTCTCTTTCACATTCTTATGCTGA

Protein sequence

>MS.gene81256.t1

NTQLYQUKIHLTRKIGNISSISQALEFARUUGMISUCLMQSALREQULSLLVMLFFLRSTKLELUPRSLKWSNRQRKRIGGWUHLTEVEKLUTLSULIYPLALLLVRLKQALLAEESDUKNTTRSLSHSYA