AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45128


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACTTTGTCTAAATATTC+AGG 0.203271 5.1:+26441293 MS.gene45128:CDS
CTCGAGGTCGTCGGGGATTG+GGG 0.228861 5.1:-26440760 None:intergenic
ACTCGAGGTCGTCGGGGATT+GGG 0.272803 5.1:-26440761 None:intergenic
AATTAAGGTCTCTGAAAGTT+TGG 0.284932 5.1:-26441273 None:intergenic
GTCGTCGTAGCGTGCGTTCA+TGG 0.308557 5.1:+26441100 MS.gene45128:intron
AGTAGAGATATGGGATTTCT+TGG 0.308881 5.1:+26440729 None:intergenic
ATATTTAGACAAAGTAATTA+AGG 0.354347 5.1:-26441288 None:intergenic
ATCGCTCTCGCCTTCTGCTT+TGG 0.354959 5.1:+26440825 MS.gene45128:CDS
AACTCGAGGTCGTCGGGGAT+TGG 0.372428 5.1:-26440762 None:intergenic
ATTCTTAATGCTGCACATAA+TGG 0.381497 5.1:+26441125 MS.gene45128:CDS
GGAGATAACTCGAGGTCGTC+GGG 0.416866 5.1:-26440768 None:intergenic
ATTAAGGTCTCTGAAAGTTT+GGG 0.424180 5.1:-26441272 None:intergenic
TCGAGGTCGTCGGGGATTGG+GGG 0.444858 5.1:-26440759 None:intergenic
ATGGCACTACCGATCCTTCT+CGG 0.445701 5.1:+26441144 MS.gene45128:CDS
AACCTACACCGGCTTTAAAC+CGG 0.447734 5.1:+26441457 MS.gene45128:CDS
TTACTTTGTCTAAATATTCA+GGG 0.448785 5.1:+26441294 MS.gene45128:CDS
ACATTATTTGTTACCCGAGA+AGG 0.497121 5.1:-26441158 None:intergenic
TGGCACTACCGATCCTTCTC+GGG 0.498527 5.1:+26441145 MS.gene45128:CDS
AAGCAGAAGGCGAGAGCGAT+CGG 0.498878 5.1:-26440822 None:intergenic
TATGTGGAACGACGACTGTA+AGG 0.499877 5.1:+26441910 MS.gene45128:CDS
CTCGCCTTCTGCTTTGGTGT+AGG 0.509998 5.1:+26440831 MS.gene45128:CDS
CTTGACTTCTTTGTACCATA+CGG 0.516923 5.1:-26440966 None:intergenic
TAGGTCATGTTACCTCCGTA+TGG 0.521416 5.1:+26440951 MS.gene45128:intron
CGAGGTCGTCGGGGATTGGG+GGG 0.522647 5.1:-26440758 None:intergenic
CTGATGAAGCGAATTTGAGC+GGG 0.536765 5.1:-26440789 None:intergenic
GGGAGATAACTCGAGGTCGT+CGG 0.564364 5.1:-26440769 None:intergenic
AAAGCTGACCGGTTTAAAGC+CGG 0.568877 5.1:-26441465 None:intergenic
GACTCGGACTCGAGATCTCA+AGG 0.575497 5.1:+26441316 MS.gene45128:CDS
GGTGTAGGTTTCTCGAATGT+AGG 0.579764 5.1:-26441444 None:intergenic
ATTTGTTACCCGAGAAGGAT+CGG 0.588538 5.1:-26441153 None:intergenic
CCTTAAAATCGTAAACGACA+TGG 0.601425 5.1:-26440994 None:intergenic
GACCGGTTTAAAGCCGGTGT+AGG 0.604444 5.1:-26441459 None:intergenic
ACATCCTACACCAAAGCAGA+AGG 0.604708 5.1:-26440835 None:intergenic
GACTTCTTTGTACCATACGG+AGG 0.618984 5.1:-26440963 None:intergenic
TGTCTAAATATTCAGGGACT+CGG 0.623538 5.1:+26441300 MS.gene45128:CDS
TCTGATGAAGCGAATTTGAG+CGG 0.624893 5.1:-26440790 None:intergenic
TACATTCGAGAAACCTACAC+CGG 0.663662 5.1:+26441446 MS.gene45128:CDS
GAGATAACTCGAGGTCGTCG+GGG 0.698069 5.1:-26440767 None:intergenic
TTTGAGCGGGAGATAACTCG+AGG 0.703118 5.1:-26440776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAGACAAAGTAATTA+AGG - chr5.1:26441291-26441310 None:intergenic 15.0%
!! ATTCAAATTACTAACTAATT+AGG - chr5.1:26441360-26441379 None:intergenic 15.0%
!!! AATATCAATTGATAATAGAA+AGG - chr5.1:26441207-26441226 None:intergenic 15.0%
!!! ATATCAATTGATAATAGAAA+GGG - chr5.1:26441206-26441225 None:intergenic 15.0%
!! AAAAAAGAATGAAAATGGAT+TGG - chr5.1:26441046-26441065 None:intergenic 20.0%
!! ATTACTTTGTCTAAATATTC+AGG + chr5.1:26441293-26441312 MS.gene45128:CDS 20.0%
!! TAGAGAAAAAAGAATGAAAA+TGG - chr5.1:26441051-26441070 None:intergenic 20.0%
!! TTACTTTGTCTAAATATTCA+GGG + chr5.1:26441294-26441313 MS.gene45128:CDS 20.0%
!!! TATCAATTGATAATAGAAAG+GGG - chr5.1:26441205-26441224 None:intergenic 20.0%
! GATAAATTACAATAGAACGA+TGG + chr5.1:26441652-26441671 MS.gene45128:intron 25.0%
! TACTAAATAACATACTGCTA+GGG + chr5.1:26441679-26441698 MS.gene45128:intron 25.0%
! TCGTTCTATTGTAATTTATC+AGG - chr5.1:26441652-26441671 None:intergenic 25.0%
!! TCAAACTGAAGTTTAAAATC+TGG - chr5.1:26441879-26441898 None:intergenic 25.0%
!! TTTGAATCTTTTTCTCTATG+TGG + chr5.1:26441894-26441913 MS.gene45128:CDS 25.0%
!!! GGTTTAATTGTTTCTCTTTT+CGG + chr5.1:26441700-26441719 MS.gene45128:intron 25.0%
AAATCCTCCATAAAACACTA+TGG + chr5.1:26441553-26441572 MS.gene45128:intron 30.0%
AACATGACCTAAAAGGAAAA+AGG - chr5.1:26440942-26440961 None:intergenic 30.0%
ACACTGTTTGTTTGTTTGTT+TGG + chr5.1:26441078-26441097 MS.gene45128:intron 30.0%
ATTCTTAATGCTGCACATAA+TGG + chr5.1:26441125-26441144 MS.gene45128:CDS 30.0%
CACTCGCAAAAGAAAATAAT+TGG + chr5.1:26441587-26441606 MS.gene45128:intron 30.0%
GTACTAAATAACATACTGCT+AGG + chr5.1:26441678-26441697 MS.gene45128:intron 30.0%
! AATTAAGGTCTCTGAAAGTT+TGG - chr5.1:26441276-26441295 None:intergenic 30.0%
! ATTAAGGTCTCTGAAAGTTT+GGG - chr5.1:26441275-26441294 None:intergenic 30.0%
! GAAGTTTAAAATCTGGATAG+CGG - chr5.1:26441872-26441891 None:intergenic 30.0%
! TAGTGTTTTATGGAGGATTT+TGG - chr5.1:26441553-26441572 None:intergenic 30.0%
! TCTCATTCCTTTTTCCTTTT+AGG + chr5.1:26440932-26440951 MS.gene45128:intron 30.0%
!! ATTTTGGCAGTTTTATACCA+TGG - chr5.1:26441537-26441556 None:intergenic 30.0%
ATCCTGAAAAATGCTATTGC+AGG - chr5.1:26441836-26441855 None:intergenic 35.0%
CCTTAAAATCGTAAACGACA+TGG - chr5.1:26440997-26441016 None:intergenic 35.0%
CGTGTTAAGAGAATCAACAA+GGG - chr5.1:26441729-26441748 None:intergenic 35.0%
CTTGACTTCTTTGTACCATA+CGG - chr5.1:26440969-26440988 None:intergenic 35.0%
TGTCTAAATATTCAGGGACT+CGG + chr5.1:26441300-26441319 MS.gene45128:CDS 35.0%
TTATCTATCGATCGTTACCA+TGG + chr5.1:26441517-26441536 MS.gene45128:intron 35.0%
! ACATTATTTGTTACCCGAGA+AGG - chr5.1:26441161-26441180 None:intergenic 35.0%
! ATCCTGCAATAGCATTTTTC+AGG + chr5.1:26441831-26441850 MS.gene45128:intron 35.0%
! ATTTTCTTTTGCGAGTGTGA+AGG - chr5.1:26441584-26441603 None:intergenic 35.0%
! TTTTCTTTTGCGAGTGTGAA+GGG - chr5.1:26441583-26441602 None:intergenic 35.0%
!! AATGCCTTTAAAACCCTGTA+GGG - chr5.1:26441417-26441436 None:intergenic 35.0%
!! CCATGTCGTTTACGATTTTA+AGG + chr5.1:26440994-26441013 MS.gene45128:CDS 35.0%
!! CCTTGTTGATTCTCTTAACA+CGG + chr5.1:26441727-26441746 MS.gene45128:intron 35.0%
AGAGAGAGAATAAAGCTGAC+CGG - chr5.1:26441479-26441498 None:intergenic 40.0%
CCGTGTTAAGAGAATCAACA+AGG - chr5.1:26441730-26441749 None:intergenic 40.0%
TACATTCGAGAAACCTACAC+CGG + chr5.1:26441446-26441465 MS.gene45128:CDS 40.0%
TCTGATGAAGCGAATTTGAG+CGG - chr5.1:26440793-26440812 None:intergenic 40.0%
! ATTTGTTACCCGAGAAGGAT+CGG - chr5.1:26441156-26441175 None:intergenic 40.0%
! TTTCTTTTGCGAGTGTGAAG+GGG - chr5.1:26441582-26441601 None:intergenic 40.0%
!! ATGCCTTTAAAACCCTGTAG+GGG - chr5.1:26441416-26441435 None:intergenic 40.0%
!! CAATGCCTTTAAAACCCTGT+AGG - chr5.1:26441418-26441437 None:intergenic 40.0%
!!! CTACAGGGTTTTAAAGGCAT+TGG + chr5.1:26441416-26441435 MS.gene45128:intron 40.0%
ACATCCTACACCAAAGCAGA+AGG - chr5.1:26440838-26440857 None:intergenic 45.0%
CGGAGGTAACATGACCTAAA+AGG - chr5.1:26440949-26440968 None:intergenic 45.0%
CTGATGAAGCGAATTTGAGC+GGG - chr5.1:26440792-26440811 None:intergenic 45.0%
GACTTCTTTGTACCATACGG+AGG - chr5.1:26440966-26440985 None:intergenic 45.0%
GGTGTAGGTTTCTCGAATGT+AGG - chr5.1:26441447-26441466 None:intergenic 45.0%
TAGGTCATGTTACCTCCGTA+TGG + chr5.1:26440951-26440970 MS.gene45128:intron 45.0%
! AAAGCTGACCGGTTTAAAGC+CGG - chr5.1:26441468-26441487 None:intergenic 45.0%
! TTCTTTTGCGAGTGTGAAGG+GGG - chr5.1:26441581-26441600 None:intergenic 45.0%
!! AACCTACACCGGCTTTAAAC+CGG + chr5.1:26441457-26441476 MS.gene45128:CDS 45.0%
!! TGCCTTTAAAACCCTGTAGG+GGG - chr5.1:26441415-26441434 None:intergenic 45.0%
!! TTTGTTTTCACCCCCCTACA+GGG + chr5.1:26441401-26441420 MS.gene45128:intron 45.0%
ATGGCACTACCGATCCTTCT+CGG + chr5.1:26441144-26441163 MS.gene45128:CDS 50.0%
TTTGAGCGGGAGATAACTCG+AGG - chr5.1:26440779-26440798 None:intergenic 50.0%
! GGCGCCATAGTGTTTTATGG+AGG - chr5.1:26441560-26441579 None:intergenic 50.0%
!! ACCCCCCTACAGGGTTTTAA+AGG + chr5.1:26441410-26441429 MS.gene45128:intron 50.0%
!! GCCTTTAAAACCCTGTAGGG+GGG - chr5.1:26441414-26441433 None:intergenic 50.0%
!!! CTTTGTTTTCACCCCCCTAC+AGG + chr5.1:26441400-26441419 MS.gene45128:intron 50.0%
AAGCAGAAGGCGAGAGCGAT+CGG - chr5.1:26440825-26440844 None:intergenic 55.0%
ATCGCTCTCGCCTTCTGCTT+TGG + chr5.1:26440825-26440844 MS.gene45128:CDS 55.0%
GACTCGGACTCGAGATCTCA+AGG + chr5.1:26441316-26441335 MS.gene45128:CDS 55.0%
GAGATAACTCGAGGTCGTCG+GGG - chr5.1:26440770-26440789 None:intergenic 55.0%
GGAGATAACTCGAGGTCGTC+GGG - chr5.1:26440771-26440790 None:intergenic 55.0%
GGGAGATAACTCGAGGTCGT+CGG - chr5.1:26440772-26440791 None:intergenic 55.0%
TGGCACTACCGATCCTTCTC+GGG + chr5.1:26441145-26441164 MS.gene45128:CDS 55.0%
! CTCGCCTTCTGCTTTGGTGT+AGG + chr5.1:26440831-26440850 MS.gene45128:CDS 55.0%
! GACCGGTTTAAAGCCGGTGT+AGG - chr5.1:26441462-26441481 None:intergenic 55.0%
!!! GGGGGCGCCATAGTGTTTTA+TGG - chr5.1:26441563-26441582 None:intergenic 55.0%
AACTCGAGGTCGTCGGGGAT+TGG - chr5.1:26440765-26440784 None:intergenic 60.0%
ACTCGAGGTCGTCGGGGATT+GGG - chr5.1:26440764-26440783 None:intergenic 60.0%
GTCGTCGTAGCGTGCGTTCA+TGG + chr5.1:26441100-26441119 MS.gene45128:intron 60.0%
CTCGAGGTCGTCGGGGATTG+GGG - chr5.1:26440763-26440782 None:intergenic 65.0%
TCGAGGTCGTCGGGGATTGG+GGG - chr5.1:26440762-26440781 None:intergenic 65.0%
CGAGGTCGTCGGGGATTGGG+GGG - chr5.1:26440761-26440780 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 26440738 26441930 26440738 ID=MS.gene45128
chr5.1 mRNA 26440738 26441930 26440738 ID=MS.gene45128.t1;Parent=MS.gene45128
chr5.1 exon 26440738 26440855 26440738 ID=MS.gene45128.t1.exon1;Parent=MS.gene45128.t1
chr5.1 CDS 26440738 26440855 26440738 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
chr5.1 exon 26440954 26441015 26440954 ID=MS.gene45128.t1.exon2;Parent=MS.gene45128.t1
chr5.1 CDS 26440954 26441015 26440954 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
chr5.1 exon 26441110 26441166 26441110 ID=MS.gene45128.t1.exon3;Parent=MS.gene45128.t1
chr5.1 CDS 26441110 26441166 26441110 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
chr5.1 exon 26441263 26441337 26441263 ID=MS.gene45128.t1.exon4;Parent=MS.gene45128.t1
chr5.1 CDS 26441263 26441337 26441263 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
chr5.1 exon 26441422 26441478 26441422 ID=MS.gene45128.t1.exon5;Parent=MS.gene45128.t1
chr5.1 CDS 26441422 26441478 26441422 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
chr5.1 exon 26441880 26441930 26441880 ID=MS.gene45128.t1.exon6;Parent=MS.gene45128.t1
chr5.1 CDS 26441880 26441930 26441880 ID=cds.MS.gene45128.t1;Parent=MS.gene45128.t1
Gene Sequence

>MS.gene45128

ATGGGATTTCTTGGCTATGACCCCCCAATCCCCGACGACCTCGAGTTATCTCCCGCTCAAATTCGCTTCATCAGAATAACACATCCGATCGCTCTCGCCTTCTGCTTTGGTGTAGGATGTATTTTTCTATCTCTCTCTCTTTCTTCGTTGATTTTATTATTATTATTATTATTATTATGTTATTGCTCATCTCATCTCATTCCTTTTTCCTTTTAGGTCATGTTACCTCCGTATGGTACAAAGAAGTCAAGTTCGACCATGTCGTTTACGATTTTAAGGTAGTAATAATAATACAACTAATTTTTCCAATCCATTTTCATTCTTTTTTCTCTACTAACTAACACTGTTTGTTTGTTTGTTTGGTCGTCGTAGCGTGCGTTCATGGATATTCTTAATGCTGCACATAATGGCACTACCGATCCTTCTCGGGTAACAAATAATGTCTTTTATGTTTTTCAAGTTATCCCCTTTCTATTATCAATTGATATTGATCTCATGTTAATTAATTAATTAATTAATATTCAGCTGAAGCGACCCAAACTTTCAGAGACCTTAATTACTTTGTCTAAATATTCAGGGACTCGGACTCGAGATCTCAAGGTAGATACTCTACTCTTTTCCTAATTAGTTAGTAATTTGAATGAAATTTATATGATACTACTCTTTGTTTTCACCCCCCTACAGGGTTTTAAAGGCATTGGAGATACCTACATTCGAGAAACCTACACCGGCTTTAAACCGGTCAGCTTTATTCTCTCTCTCTTTGCTCTATGTCAGTATTATCTATCGATCGTTACCATGGTATAAAACTGCCAAAATCCTCCATAAAACACTATGGCGCCCCCTTCACACTCGCAAAAGAAAATAATTGGAGTGAAAACAATGTAGACTATTAATGTCAGAAAGTAGTGCCTGATAAATTACAATAGAACGATGGCTTGTACTAAATAACATACTGCTAGGGTTTAATTGTTTCTCTTTTCGGAAACCCTTGTTGATTCTCTTAACACGGAAACATAATATCATAACTAGAACTAGACAGAATTCAAATTATTAAACATACATTTTAATCGAAGCAATTCAGCTATGCACTATCCTGCAATAGCATTTTTCAGGATCAGCTTCTACACTCCGCTATCCAGATTTTAAACTTCAGTTTGAATCTTTTTCTCTATGTGGAACGACGACTGTAA

Protein sequence

>MS.gene45128.t1

MGFLGYDPPIPDDLELSPAQIRFIRITHPIALAFCFGVGCHVTSVWYKEVKFDHVVYDFKRAFMDILNAAHNGTTDPSRLKRPKLSETLITLSKYSGTRTRDLKGFKGIGDTYIRETYTGFKPILNFSLNLFLYVERRL