AlfalfaGEDB | MS.gene061872

M. sativa cultivar XinJiangDaYe / MS.gene061872

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061872.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061872.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061872.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene061872	0.85677	2.57E-62	-1.69E-46
MS.gene049159	MS.gene061872	0.835768	1.38E-56	-1.69E-46
MS.gene049254	MS.gene061872	0.804042	2.61E-49	-1.69E-46
MS.gene049255	MS.gene061872	0.825883	3.68E-54	-1.69E-46
MS.gene049341	MS.gene061872	0.826643	2.42E-54	-1.69E-46
MS.gene049343	MS.gene061872	0.814272	1.68E-51	-1.69E-46
MS.gene049350	MS.gene061872	0.847087	1.44E-59	-1.69E-46
MS.gene049404	MS.gene061872	0.805867	1.09E-49	-1.69E-46
MS.gene049501	MS.gene061872	0.82742	1.58E-54	-1.69E-46
MS.gene049672	MS.gene061872	0.801072	1.07E-48	-1.69E-46
MS.gene049711	MS.gene061872	0.806262	8.96E-50	-1.69E-46
MS.gene049821	MS.gene061872	0.883991	2.81E-71	-1.69E-46
MS.gene049825	MS.gene061872	0.806145	9.48E-50	-1.69E-46
MS.gene049850	MS.gene061872	0.875102	4.01E-68	-1.69E-46
MS.gene049873	MS.gene061872	0.832498	9.13E-56	-1.69E-46
MS.gene050020	MS.gene061872	0.854519	1.17E-61	-1.69E-46
MS.gene050156	MS.gene061872	0.806328	8.68E-50	-1.69E-46
MS.gene050273	MS.gene061872	0.804067	2.58E-49	-1.69E-46
MS.gene05045	MS.gene061872	0.848279	6.78E-60	-1.69E-46
MS.gene050689	MS.gene061872	0.846436	2.18E-59	-1.69E-46
MS.gene050807	MS.gene061872	0.845785	3.27E-59	-1.69E-46
MS.gene050808	MS.gene061872	0.803294	3.73E-49	-1.69E-46
MS.gene050815	MS.gene061872	0.812208	4.76E-51	-1.69E-46
MS.gene050844	MS.gene061872	0.801463	8.90E-49	-1.69E-46
MS.gene050901	MS.gene061872	0.888903	3.90E-73	-1.69E-46
MS.gene051018	MS.gene061872	0.827987	1.15E-54	-1.69E-46
MS.gene051180	MS.gene061872	0.804488	2.11E-49	-1.69E-46
MS.gene051198	MS.gene061872	0.801974	6.99E-49	-1.69E-46
MS.gene051199	MS.gene061872	0.80524	1.47E-49	-1.69E-46
MS.gene051247	MS.gene061872	0.878638	2.39E-69	-1.69E-46
MS.gene051279	MS.gene061872	0.807745	4.35E-50	-1.69E-46
MS.gene051328	MS.gene061872	0.873027	2.02E-67	-1.69E-46
MS.gene05134	MS.gene061872	0.82463	7.27E-54	-1.69E-46
MS.gene051388	MS.gene061872	0.816619	5.04E-52	-1.69E-46
MS.gene051490	MS.gene061872	0.801241	9.88E-49	-1.69E-46
MS.gene05157	MS.gene061872	0.857067	2.10E-62	-1.69E-46
MS.gene051664	MS.gene061872	0.83153	1.58E-55	-1.69E-46
MS.gene051700	MS.gene061872	0.841384	4.91E-58	-1.69E-46
MS.gene051719	MS.gene061872	0.878434	2.82E-69	-1.69E-46
MS.gene051720	MS.gene061872	0.882935	6.86E-71	-1.69E-46
MS.gene051744	MS.gene061872	0.86366	2.12E-64	-1.69E-46
MS.gene05188	MS.gene061872	0.804838	1.78E-49	-1.69E-46
MS.gene051957	MS.gene061872	0.823279	1.51E-53	-1.69E-46
MS.gene051975	MS.gene061872	0.814573	1.44E-51	-1.69E-46
MS.gene052012	MS.gene061872	0.806809	6.87E-50	-1.69E-46
MS.gene052020	MS.gene061872	0.832659	8.33E-56	-1.69E-46
MS.gene052133	MS.gene061872	0.846844	1.68E-59	-1.69E-46
MS.gene052516	MS.gene061872	0.861373	1.07E-63	-1.69E-46
MS.gene052519	MS.gene061872	0.862003	6.89E-64	-1.69E-46
MS.gene052582	MS.gene061872	0.82519	5.36E-54	-1.69E-46
MS.gene052584	MS.gene061872	0.82874	7.60E-55	-1.69E-46
MS.gene052611	MS.gene061872	0.846624	1.93E-59	-1.69E-46
MS.gene052612	MS.gene061872	0.806871	6.67E-50	-1.69E-46
MS.gene052665	MS.gene061872	0.80734	5.30E-50	-1.69E-46
MS.gene052773	MS.gene061872	0.852219	5.33E-61	-1.69E-46
MS.gene052863	MS.gene061872	0.85577	5.05E-62	-1.69E-46
MS.gene052879	MS.gene061872	0.834723	2.54E-56	-1.69E-46
MS.gene052880	MS.gene061872	0.827739	1.32E-54	-1.69E-46
MS.gene052896	MS.gene061872	0.81862	1.78E-52	-1.69E-46
MS.gene052947	MS.gene061872	0.816403	5.63E-52	-1.69E-46
MS.gene053008	MS.gene061872	0.831538	1.58E-55	-1.69E-46
MS.gene053122	MS.gene061872	0.853248	2.71E-61	-1.69E-46
MS.gene053144	MS.gene061872	0.839607	1.43E-57	-1.69E-46
MS.gene053226	MS.gene061872	0.883633	3.80E-71	-1.69E-46
MS.gene053296	MS.gene061872	0.805998	1.02E-49	-1.69E-46
MS.gene053542	MS.gene061872	0.800634	1.31E-48	-1.69E-46
MS.gene053563	MS.gene061872	0.818786	1.63E-52	-1.69E-46
MS.gene053581	MS.gene061872	0.871026	9.35E-67	-1.69E-46
MS.gene053599	MS.gene061872	0.831797	1.36E-55	-1.69E-46
MS.gene05364	MS.gene061872	0.826942	2.06E-54	-1.69E-46
MS.gene053883	MS.gene061872	0.837042	6.56E-57	-1.69E-46
MS.gene053900	MS.gene061872	0.811944	5.43E-51	-1.69E-46
MS.gene054142	MS.gene061872	0.806633	7.48E-50	-1.69E-46
MS.gene05415	MS.gene061872	0.814648	1.38E-51	-1.69E-46
MS.gene054236	MS.gene061872	0.827687	1.36E-54	-1.69E-46
MS.gene05423	MS.gene061872	0.822613	2.16E-53	-1.69E-46
MS.gene054472	MS.gene061872	0.819091	1.39E-52	-1.69E-46
MS.gene054584	MS.gene061872	0.833369	5.55E-56	-1.69E-46
MS.gene05458	MS.gene061872	0.821793	3.34E-53	-1.69E-46
MS.gene054651	MS.gene061872	0.868857	4.78E-66	-1.69E-46
MS.gene054775	MS.gene061872	0.847718	9.69E-60	-1.69E-46
MS.gene054801	MS.gene061872	0.807935	3.96E-50	-1.69E-46
MS.gene055144	MS.gene061872	0.809242	2.08E-50	-1.69E-46
MS.gene05517	MS.gene061872	0.812628	3.85E-51	-1.69E-46
MS.gene055228	MS.gene061872	0.802382	5.76E-49	-1.69E-46
MS.gene055465	MS.gene061872	0.871482	6.61E-67	-1.69E-46
MS.gene055466	MS.gene061872	0.900641	5.98E-78	-1.69E-46
MS.gene055468	MS.gene061872	0.874198	8.15E-68	-1.69E-46
MS.gene055469	MS.gene061872	0.816541	5.24E-52	-1.69E-46
MS.gene055512	MS.gene061872	0.840081	1.08E-57	-1.69E-46
MS.gene055513	MS.gene061872	0.842288	2.83E-58	-1.69E-46
MS.gene055537	MS.gene061872	0.838313	3.10E-57	-1.69E-46
MS.gene055580	MS.gene061872	-0.866759	2.25E-65	-1.69E-46
MS.gene055589	MS.gene061872	0.802262	6.10E-49	-1.69E-46
MS.gene055694	MS.gene061872	0.814004	1.92E-51	-1.69E-46
MS.gene055778	MS.gene061872	0.832349	9.94E-56	-1.69E-46
MS.gene055818	MS.gene061872	0.856906	2.34E-62	-1.69E-46
MS.gene055890	MS.gene061872	0.806886	6.61E-50	-1.69E-46
MS.gene055891	MS.gene061872	0.809245	2.08E-50	-1.69E-46
MS.gene055926	MS.gene061872	0.802622	5.14E-49	-1.69E-46
MS.gene056005	MS.gene061872	0.853189	2.82E-61	-1.69E-46
MS.gene056043	MS.gene061872	-0.842913	1.93E-58	-1.69E-46
MS.gene05604	MS.gene061872	0.800581	1.35E-48	-1.69E-46
MS.gene056050	MS.gene061872	0.824411	8.19E-54	-1.69E-46
MS.gene05608	MS.gene061872	0.849807	2.55E-60	-1.69E-46
MS.gene056173	MS.gene061872	0.814387	1.58E-51	-1.69E-46
MS.gene056174	MS.gene061872	0.818876	1.56E-52	-1.69E-46
MS.gene056215	MS.gene061872	0.885414	8.30E-72	-1.69E-46
MS.gene056308	MS.gene061872	0.871147	8.53E-67	-1.69E-46
MS.gene056366	MS.gene061872	0.862555	4.66E-64	-1.69E-46
MS.gene056545	MS.gene061872	0.803368	3.61E-49	-1.69E-46
MS.gene056553	MS.gene061872	0.818172	2.25E-52	-1.69E-46
MS.gene056595	MS.gene061872	0.861508	9.76E-64	-1.69E-46
MS.gene057034	MS.gene061872	0.837493	5.03E-57	-1.69E-46
MS.gene057060	MS.gene061872	0.812758	3.61E-51	-1.69E-46
MS.gene057132	MS.gene061872	0.800265	1.56E-48	-1.69E-46
MS.gene057208	MS.gene061872	0.810941	8.97E-51	-1.69E-46
MS.gene057294	MS.gene061872	0.816548	5.23E-52	-1.69E-46
MS.gene057314	MS.gene061872	0.807489	4.93E-50	-1.69E-46
MS.gene057444	MS.gene061872	0.843945	1.03E-58	-1.69E-46
MS.gene057446	MS.gene061872	0.813574	2.39E-51	-1.69E-46
MS.gene057693	MS.gene061872	0.848665	5.30E-60	-1.69E-46
MS.gene057694	MS.gene061872	0.823213	1.56E-53	-1.69E-46
MS.gene057705	MS.gene061872	0.830583	2.71E-55	-1.69E-46
MS.gene057855	MS.gene061872	0.806905	6.56E-50	-1.69E-46
MS.gene057915	MS.gene061872	0.856933	2.30E-62	-1.69E-46
MS.gene058010	MS.gene061872	0.825714	4.03E-54	-1.69E-46
MS.gene058037	MS.gene061872	0.813638	2.31E-51	-1.69E-46
MS.gene058339	MS.gene061872	0.827829	1.26E-54	-1.69E-46
MS.gene05847	MS.gene061872	0.836883	7.21E-57	-1.69E-46
MS.gene058625	MS.gene061872	0.858133	1.02E-62	-1.69E-46
MS.gene058668	MS.gene061872	0.803378	3.59E-49	-1.69E-46
MS.gene058783	MS.gene061872	0.820171	7.90E-53	-1.69E-46
MS.gene058852	MS.gene061872	0.807932	3.97E-50	-1.69E-46
MS.gene05894	MS.gene061872	0.83714	6.20E-57	-1.69E-46
MS.gene058979	MS.gene061872	0.821431	4.05E-53	-1.69E-46
MS.gene059015	MS.gene061872	0.805942	1.05E-49	-1.69E-46
MS.gene059084	MS.gene061872	0.804746	1.86E-49	-1.69E-46
MS.gene05950	MS.gene061872	0.803208	3.89E-49	-1.69E-46
MS.gene05954	MS.gene061872	0.821594	3.72E-53	-1.69E-46
MS.gene059625	MS.gene061872	0.841079	5.91E-58	-1.69E-46
MS.gene05978	MS.gene061872	0.832032	1.19E-55	-1.69E-46
MS.gene059842	MS.gene061872	0.873573	1.32E-67	-1.69E-46
MS.gene059937	MS.gene061872	0.82139	4.14E-53	-1.69E-46
MS.gene060194	MS.gene061872	0.801563	8.49E-49	-1.69E-46
MS.gene060310	MS.gene061872	0.814072	1.86E-51	-1.69E-46
MS.gene060355	MS.gene061872	0.805397	1.36E-49	-1.69E-46
MS.gene060365	MS.gene061872	0.831292	1.81E-55	-1.69E-46
MS.gene060429	MS.gene061872	0.892399	1.65E-74	-1.69E-46
MS.gene060519	MS.gene061872	0.839342	1.68E-57	-1.69E-46
MS.gene060521	MS.gene061872	0.816549	5.22E-52	-1.69E-46
MS.gene060587	MS.gene061872	0.869846	2.28E-66	-1.69E-46
MS.gene060689	MS.gene061872	0.801582	8.41E-49	-1.69E-46
MS.gene060803	MS.gene061872	0.841305	5.15E-58	-1.69E-46
MS.gene060917	MS.gene061872	0.829041	6.43E-55	-1.69E-46
MS.gene061100	MS.gene061872	0.804021	2.64E-49	-1.69E-46
MS.gene061176	MS.gene061872	0.851564	8.18E-61	-1.69E-46
MS.gene061177	MS.gene061872	0.852003	6.14E-61	-1.69E-46
MS.gene061178	MS.gene061872	0.851462	8.74E-61	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene061872	MS.gene04452	PPI
MS.gene061872	MS.gene070244	PPI
MS.gene061872	MS.gene038469	PPI
MS.gene061872	MS.gene061867	PPI
MS.gene061872	MS.gene008276	PPI
MS.gene061872	MS.gene30920	PPI
MS.gene061872	MS.gene05619	PPI
MS.gene061872	MS.gene73136	PPI
MS.gene061872	MS.gene049821	PPI
MS.gene30920	MS.gene061872	PPI
MS.gene061872	MS.gene04068	PPI
MS.gene061872	MS.gene022946	PPI
MS.gene061872	MS.gene035719	PPI
MS.gene061872	MS.gene05623	PPI
MS.gene061872	MS.gene89488	PPI
MS.gene061872	MS.gene66225	PPI
MS.gene061872	MS.gene035720	PPI
MS.gene061872	MS.gene70955	PPI
MS.gene061872	MS.gene61017	PPI
MS.gene070241	MS.gene061872	PPI
MS.gene061872	MS.gene09871	PPI
MS.gene061872	MS.gene012390	PPI
MS.gene061872	MS.gene08121	PPI
MS.gene061872	MS.gene064557	PPI
MS.gene061876	MS.gene061872	PPI
MS.gene061872	MS.gene04434	PPI
MS.gene061872	MS.gene070907	PPI
MS.gene061872	MS.gene04392	PPI
MS.gene061872	MS.gene89487	PPI
MS.gene012391	MS.gene061872	PPI
MS.gene070244	MS.gene061872	PPI
MS.gene061872	MS.gene010219	PPI
MS.gene061872	MS.gene04433	PPI
MS.gene061872	MS.gene040383	PPI
MS.gene060463	MS.gene061872	PPI
MS.gene061872	MS.gene68545	PPI
MS.gene061872	MS.gene070242	PPI
MS.gene061872	MS.gene016624	PPI
MS.gene009027	MS.gene061872	PPI
MS.gene31196	MS.gene061872	PPI
MS.gene061872	MS.gene048746	PPI
MS.gene060465	MS.gene061872	PPI
MS.gene061872	MS.gene028629	PPI
MS.gene061872	MS.gene58854	PPI
MS.gene061872	MS.gene043967	PPI
MS.gene061872	MS.gene31196	PPI
MS.gene061872	MS.gene060465	PPI
MS.gene061872	MS.gene08053	PPI
MS.gene061872	MS.gene035897	PPI
MS.gene061872	MS.gene049705	PPI
MS.gene061870	MS.gene061872	PPI
MS.gene061872	MS.gene061870	PPI
MS.gene035718	MS.gene061872	PPI
MS.gene061872	MS.gene05608	PPI
MS.gene061872	MS.gene035896	PPI
MS.gene061872	MS.gene88681	PPI
MS.gene061872	MS.gene82546	PPI
MS.gene78578	MS.gene061872	PPI
MS.gene061872	MS.gene60093	PPI
MS.gene061872	MS.gene08122	PPI
MS.gene061872	MS.gene09873	PPI
MS.gene061872	MS.gene035718	PPI
MS.gene028629	MS.gene061872	PPI
MS.gene061872	MS.gene022932	PPI
MS.gene061872	MS.gene010220	PPI
MS.gene061872	MS.gene04972	PPI
MS.gene061872	MS.gene004121	PPI
MS.gene035787	MS.gene061872	PPI
MS.gene061872	MS.gene62520	PPI
MS.gene04067	MS.gene061872	PPI
MS.gene71215	MS.gene061872	PPI
MS.gene061872	MS.gene018578	PPI
MS.gene061872	MS.gene035721	PPI
MS.gene061872	MS.gene009027	PPI
MS.gene061872	MS.gene04064	PPI
MS.gene061872	MS.gene018580	PPI
MS.gene061872	MS.gene004124	PPI
MS.gene061872	MS.gene88680	PPI
MS.gene071136	MS.gene061872	PPI
MS.gene061872	MS.gene072493	PPI
MS.gene061872	MS.gene04052	PPI
MS.gene061872	MS.gene84536	PPI
MS.gene061872	MS.gene061866	PPI
MS.gene061872	MS.gene64650	PPI
MS.gene88680	MS.gene061872	PPI
MS.gene061872	MS.gene79195	PPI
MS.gene061872	MS.gene048705	PPI
MS.gene061872	MS.gene061874	PPI
MS.gene061872	MS.gene04576	PPI
MS.gene056215	MS.gene061872	PPI
MS.gene061872	MS.gene061869	PPI
MS.gene061872	MS.gene64920	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061872.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene061872.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene061872.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061872.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene061872.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGTGTTCTCAAAATCTTCT+TGG	0.286841	7.4:+10706912	MS.gene061872:CDS
GGAAGGACCCTTTACGGTTT+CGG	0.307868	7.4:+10707027	MS.gene061872:CDS
TGGAAAAGGTGGAAAAGGTT+TGG	0.419608	7.4:+10706756	MS.gene061872:CDS
GGAAAAGGTGGAAAAGGTTT+GGG	0.432725	7.4:+10706757	MS.gene061872:CDS
AGGCAGGGAAGGACCCTTTA+CGG	0.435749	7.4:+10707021	MS.gene061872:CDS
TCGCAGGCTTTGTTATGCCT+TGG	0.436279	7.4:-10706825	None:intergenic
GGTGGAAAAGGTTTGGGAAA+GGG	0.442921	7.4:+10706763	MS.gene061872:CDS
TCAACCACCGAAACCGTAAA+GGG	0.488164	7.4:-10707034	None:intergenic
CTGCGATTCGTCGTCTTGCA+AGG	0.502831	7.4:+10706842	MS.gene061872:CDS
TCAGTGCAAGCGAAAATGTC+AGG	0.509801	7.4:+10706730	None:intergenic
TGTCTATGCTCTCAAGAGGC+AGG	0.510611	7.4:+10707005	MS.gene061872:CDS
AAAATGTCAGGTCGTGGAAA+AGG	0.512942	7.4:+10706742	None:intergenic
GGAAAAGGTTTGGGAAAGGG+AGG	0.514769	7.4:+10706766	MS.gene061872:CDS
ATCAACCACCGAAACCGTAA+AGG	0.525393	7.4:-10707035	None:intergenic
GGTCGTGGAAAAGGTGGAAA+AGG	0.536368	7.4:+10706751	MS.gene061872:CDS
TTACTTACACTGAGCACGCT+AGG	0.551062	7.4:+10706956	MS.gene061872:CDS
CAAGCGAAAATGTCAGGTCG+TGG	0.551935	7.4:+10706736	None:intergenic
CTTGCAAGACGACGAATCGC+AGG	0.552192	7.4:-10706841	None:intergenic
ATGCTCTCAAGAGGCAGGGA+AGG	0.564763	7.4:+10707010	MS.gene061872:CDS
AGGTGGAAAAGGTTTGGGAA+AGG	0.583555	7.4:+10706762	MS.gene061872:CDS
GTCTATGCTCTCAAGAGGCA+GGG	0.585715	7.4:+10707006	MS.gene061872:CDS
GAGAAAGACTGTTACCGCCA+TGG	0.588179	7.4:+10706978	MS.gene061872:CDS
ATGTCAGGTCGTGGAAAAGG+TGG	0.588325	7.4:+10706745	MS.gene061872:CDS
AGGAGCAAAGAGACACAGAA+AGG	0.597296	7.4:+10706786	MS.gene061872:CDS
AGGACCCTTTACGGTTTCGG+TGG	0.602372	7.4:+10707030	MS.gene061872:CDS
CGTCGTCTTGCAAGGCGTGG+TGG	0.615268	7.4:+10706850	MS.gene061872:CDS
ATTCGTCGTCTTGCAAGGCG+TGG	0.619919	7.4:+10706847	MS.gene061872:CDS
GTTCTTCGCGATAACATCCA+AGG	0.633409	7.4:+10706808	MS.gene061872:CDS
GAGAGCATAGACAACATCCA+TGG	0.645428	7.4:-10706995	None:intergenic
ATGTTGTCTATGCTCTCAAG+AGG	0.677658	7.4:+10707001	MS.gene061872:CDS
GGTGGTGTGAAGAGAATCAG+TGG	0.694412	7.4:+10706868	MS.gene061872:CDS
CTCATCTATGAAGAGACTCG+CGG	0.697662	7.4:+10706892	MS.gene061872:CDS
AGCATAGACAACATCCATGG+CGG	0.735882	7.4:-10706992	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGTTGTCTATGCTCTCAAG+AGG	+	chr7.4:10707001-10707020	MS.gene061872:CDS	40.0%
	CGGTGTTCTCAAAATCTTCT+TGG	+	chr7.4:10706912-10706931	MS.gene061872:CDS	40.0%
	GGAAAAGGTGGAAAAGGTTT+GGG	+	chr7.4:10706757-10706776	MS.gene061872:CDS	40.0%
	TGGAAAAGGTGGAAAAGGTT+TGG	+	chr7.4:10706756-10706775	MS.gene061872:CDS	40.0%
	AGCATAGACAACATCCATGG+CGG	-	chr7.4:10706995-10707014	None:intergenic	45.0%
	AGGAGCAAAGAGACACAGAA+AGG	+	chr7.4:10706786-10706805	MS.gene061872:CDS	45.0%
	AGGTGGAAAAGGTTTGGGAA+AGG	+	chr7.4:10706762-10706781	MS.gene061872:CDS	45.0%
	CTCATCTATGAAGAGACTCG+CGG	+	chr7.4:10706892-10706911	MS.gene061872:CDS	45.0%
	GGTGGAAAAGGTTTGGGAAA+GGG	+	chr7.4:10706763-10706782	MS.gene061872:CDS	45.0%
	GTTCTTCGCGATAACATCCA+AGG	+	chr7.4:10706808-10706827	MS.gene061872:CDS	45.0%
	TCAACCACCGAAACCGTAAA+GGG	-	chr7.4:10707037-10707056	None:intergenic	45.0%
	TTACTTACACTGAGCACGCT+AGG	+	chr7.4:10706956-10706975	MS.gene061872:CDS	45.0%
!	GAGAGCATAGACAACATCCA+TGG	-	chr7.4:10706998-10707017	None:intergenic	45.0%
	ATGTCAGGTCGTGGAAAAGG+TGG	+	chr7.4:10706745-10706764	MS.gene061872:CDS	50.0%
	GAGAAAGACTGTTACCGCCA+TGG	+	chr7.4:10706978-10706997	MS.gene061872:CDS	50.0%
	GGAAAAGGTTTGGGAAAGGG+AGG	+	chr7.4:10706766-10706785	MS.gene061872:CDS	50.0%
	GGAAGGACCCTTTACGGTTT+CGG	+	chr7.4:10707027-10707046	MS.gene061872:CDS	50.0%
	GGTCGTGGAAAAGGTGGAAA+AGG	+	chr7.4:10706751-10706770	MS.gene061872:CDS	50.0%
	GGTGGTGTGAAGAGAATCAG+TGG	+	chr7.4:10706868-10706887	MS.gene061872:CDS	50.0%
	GTCTATGCTCTCAAGAGGCA+GGG	+	chr7.4:10707006-10707025	MS.gene061872:CDS	50.0%
	TGTCTATGCTCTCAAGAGGC+AGG	+	chr7.4:10707005-10707024	MS.gene061872:CDS	50.0%
!!	TCGCAGGCTTTGTTATGCCT+TGG	-	chr7.4:10706828-10706847	None:intergenic	50.0%
	AGGACCCTTTACGGTTTCGG+TGG	+	chr7.4:10707030-10707049	MS.gene061872:CDS	55.0%
	AGGCAGGGAAGGACCCTTTA+CGG	+	chr7.4:10707021-10707040	MS.gene061872:CDS	55.0%
	ATGCTCTCAAGAGGCAGGGA+AGG	+	chr7.4:10707010-10707029	MS.gene061872:CDS	55.0%
	ATTCGTCGTCTTGCAAGGCG+TGG	+	chr7.4:10706847-10706866	MS.gene061872:CDS	55.0%
	CTGCGATTCGTCGTCTTGCA+AGG	+	chr7.4:10706842-10706861	MS.gene061872:CDS	55.0%
	CTTGCAAGACGACGAATCGC+AGG	-	chr7.4:10706844-10706863	None:intergenic	55.0%
	CGTCGTCTTGCAAGGCGTGG+TGG	+	chr7.4:10706850-10706869	MS.gene061872:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10706745	10707056	10706745	ID=MS.gene061872
chr7.4	mRNA	10706745	10707056	10706745	ID=MS.gene061872.t1;Parent=MS.gene061872
chr7.4	exon	10706745	10707056	10706745	ID=MS.gene061872.t1.exon1;Parent=MS.gene061872.t1
chr7.4	CDS	10706745	10707056	10706745	ID=cds.MS.gene061872.t1;Parent=MS.gene061872.t1

Gene Sequence	>MS.gene061872 ATGTCAGGTCGTGGAAAAGGTGGAAAAGGTTTGGGAAAGGGAGGAGCAAAGAGACACAGAAAGGTTCTTCGCGATAACATCCAAGGCATAACAAAGCCTGCGATTCGTCGTCTTGCAAGGCGTGGTGGTGTGAAGAGAATCAGTGGTCTCATCTATGAAGAGACTCGCGGTGTTCTCAAAATCTTCTTGGAAAACGTGATTCGCGATGCTGTTACTTACACTGAGCACGCTAGGAGAAAGACTGTTACCGCCATGGATGTTGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGTTTCGGTGGTTGA
Protein sequence	>MS.gene061872.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG