AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061314.t1 MTR_4g049910 88.367 447 23 4 1 434 1 431 0.0 778
MS.gene061314.t1 MTR_1g061970 73.289 453 100 5 2 434 21 472 0.0 654
MS.gene061314.t1 MTR_0427s0010 65.801 231 49 3 208 434 2 206 4.41e-97 290
MS.gene061314.t1 MTR_2g039960 35.052 388 215 11 61 434 41 405 1.43e-63 211
MS.gene061314.t1 MTR_2g438110 33.333 390 225 9 58 434 31 398 2.88e-63 209
MS.gene061314.t1 MTR_2g099620 35.309 388 214 11 61 434 25 389 2.51e-62 207
MS.gene061314.t1 MTR_2g099620 34.804 408 225 12 45 434 21 405 3.47e-62 207
MS.gene061314.t1 MTR_4g125970 34.704 389 215 11 61 434 41 405 9.78e-62 206
MS.gene061314.t1 MTR_4g125970 34.704 389 215 11 61 434 58 422 1.63e-61 206
MS.gene061314.t1 MTR_8g018400 33.496 409 229 12 45 434 21 405 6.57e-61 204
MS.gene061314.t1 MTR_7g076820 32.918 401 212 12 57 434 146 512 3.68e-56 194
MS.gene061314.t1 MTR_7g076800 32.170 401 215 12 57 434 137 503 5.67e-54 188
MS.gene061314.t1 MTR_7g076830 32.170 401 215 12 57 434 139 505 6.14e-54 188
MS.gene061314.t1 MTR_4g010565 29.952 414 228 14 49 434 113 492 4.37e-46 166
MS.gene061314.t1 MTR_1g012420 29.009 424 242 14 39 434 23 415 1.62e-45 163
MS.gene061314.t1 MTR_3g107420 29.567 416 235 14 45 434 32 415 4.78e-45 162
MS.gene061314.t1 MTR_6g082080 32.323 396 221 14 61 436 10 378 7.97e-42 154
MS.gene061314.t1 MTR_7g056830 31.818 396 223 14 61 436 10 378 2.15e-41 152
MS.gene061314.t1 MTR_2g100230 30.791 354 202 9 80 412 114 445 8.47e-40 149
MS.gene061314.t1 MTR_3g083690 29.002 431 253 11 12 409 99 509 4.06e-37 143
MS.gene061314.t1 MTR_2g032630 27.895 380 219 11 61 414 496 846 3.02e-36 142
MS.gene061314.t1 MTR_7g109720 28.198 383 236 9 51 414 222 584 3.67e-36 141
MS.gene061314.t1 MTR_4g105100 28.571 371 223 9 61 411 202 550 6.77e-36 140
MS.gene061314.t1 MTR_1g054630 29.877 405 232 11 37 409 133 517 8.93e-36 139
MS.gene061314.t1 MTR_2g066050 30.479 397 235 14 41 419 35 408 1.04e-35 138
MS.gene061314.t1 MTR_1g095810 30.871 379 208 11 62 411 146 499 1.34e-35 139
MS.gene061314.t1 MTR_5g027840 30.402 398 217 13 47 413 90 458 1.45e-34 135
MS.gene061314.t1 MTR_1g026830 30.079 379 211 11 62 411 148 501 1.87e-34 135
MS.gene061314.t1 MTR_7g100820 28.385 384 231 10 51 414 120 479 3.59e-34 135
MS.gene061314.t1 MTR_6g056080 29.521 376 215 10 87 436 128 479 3.65e-34 135
MS.gene061314.t1 MTR_1g102410 28.321 399 224 10 78 436 60 436 3.14e-33 132
MS.gene061314.t1 MTR_4g114120 28.611 360 203 11 81 405 159 499 9.49e-33 131
MS.gene061314.t1 MTR_2g007000 29.101 378 220 12 61 414 504 857 3.53e-32 130
MS.gene061314.t1 MTR_6g005550 26.579 380 224 10 61 414 165 515 4.88e-32 129
MS.gene061314.t1 MTR_8g027770 30.072 419 220 17 30 405 168 556 5.23e-32 129
MS.gene061314.t1 MTR_5g090120 28.926 363 199 12 101 434 118 450 1.19e-31 127
MS.gene061314.t1 MTR_1g046570 28.993 407 212 18 80 434 9 390 5.64e-31 123
MS.gene061314.t1 MTR_8g027775 30.175 401 215 19 45 405 82 457 2.01e-30 124
MS.gene061314.t1 MTR_3g467550 29.301 372 215 13 61 405 117 467 3.35e-30 123
MS.gene061314.t1 MTR_0915s0010 29.605 304 168 10 74 354 2 282 5.63e-30 117
MS.gene061314.t1 MTR_3g449250 27.318 399 230 12 64 434 93 459 6.17e-30 122
MS.gene061314.t1 MTR_3g449250 27.318 399 230 12 64 434 93 459 6.36e-30 122
MS.gene061314.t1 MTR_4g079320 28.571 378 216 15 62 414 20 368 8.57e-30 122
MS.gene061314.t1 MTR_8g027780 30.412 388 205 19 56 405 101 461 2.51e-29 120
MS.gene061314.t1 MTR_3g049990 27.184 412 245 14 45 428 132 516 2.82e-29 121
MS.gene061314.t1 MTR_8g018370 29.213 356 197 12 94 434 1 316 3.99e-29 116
MS.gene061314.t1 MTR_4g134170 27.525 396 215 9 61 412 287 654 4.82e-29 120
MS.gene061314.t1 MTR_3g467550 29.508 366 210 13 67 405 92 436 9.01e-29 119
MS.gene061314.t1 MTR_2g041140 27.295 403 227 15 58 428 138 506 1.49e-28 119
MS.gene061314.t1 MTR_6g005320 27.020 396 215 10 61 412 249 614 9.39e-28 116
MS.gene061314.t1 MTR_4g112340 30.663 362 207 13 77 414 112 453 4.09e-27 114
MS.gene061314.t1 MTR_6g033070 26.649 379 236 9 61 418 140 497 1.28e-25 109
MS.gene061314.t1 MTR_4g105100 27.160 324 205 7 69 372 209 521 1.77e-25 108
MS.gene061314.t1 MTR_2g063080 25.966 466 226 18 54 435 103 533 1.92e-25 109
MS.gene061314.t1 MTR_3g467550 28.363 342 207 11 61 375 117 447 3.22e-25 107
MS.gene061314.t1 MTR_8g097000 28.395 405 229 18 44 412 271 650 9.83e-25 107
MS.gene061314.t1 MTR_2g055850 29.134 381 224 16 58 414 88 446 1.56e-24 106
MS.gene061314.t1 MTR_4g059460 25.420 476 244 17 38 426 28 479 2.32e-24 106
MS.gene061314.t1 MTR_2g043360 25.054 463 242 17 58 433 38 482 2.18e-23 103
MS.gene061314.t1 MTR_4g068990 25.952 420 259 15 40 427 47 446 3.35e-23 102
MS.gene061314.t1 MTR_5g092560 26.250 400 247 12 53 424 60 439 7.85e-23 102
MS.gene061314.t1 MTR_8g027780 29.859 355 194 18 54 370 99 436 7.97e-22 97.8
MS.gene061314.t1 MTR_1g083130 25.481 416 221 13 61 411 26 417 1.76e-21 97.4
MS.gene061314.t1 MTR_6g039840 25.063 399 244 11 60 425 109 485 4.66e-19 90.1
MS.gene061314.t1 MTR_3g107420 28.870 239 124 10 45 254 32 253 6.95e-16 78.6
MS.gene061314.t1 MTR_5g069330 26.031 388 240 18 61 428 3 363 3.47e-15 77.4
MS.gene061314.t1 MTR_5g069330 26.031 388 240 18 61 428 7 367 3.67e-15 77.4
MS.gene061314.t1 MTR_8g032230 35.526 152 80 5 61 197 10 158 1.15e-14 72.0
MS.gene061314.t1 MTR_5g077690 23.040 421 235 17 66 411 34 440 2.78e-14 75.1
MS.gene061314.t1 MTR_4g112320 29.675 246 150 10 171 411 41 268 1.30e-13 72.4
MS.gene061314.t1 MTR_4g459350 27.984 243 125 9 38 242 25 255 1.56e-13 70.9
MS.gene061314.t1 MTR_5g092560 27.426 237 151 8 191 424 10 228 1.26e-12 70.1
MS.gene061314.t1 MTR_4g131750 24.876 402 236 16 78 434 98 478 2.26e-12 68.9
MS.gene061314.t1 MTR_2g043360 23.978 367 195 14 135 433 14 364 3.06e-12 68.6
MS.gene061314.t1 MTR_8g018330 29.651 172 99 7 268 434 15 169 4.99e-12 64.7
MS.gene061314.t1 MTR_4g068990 26.230 244 155 10 191 427 10 235 5.38e-12 68.2
MS.gene061314.t1 MTR_8g042690 24.658 365 208 18 85 414 41 373 5.22e-11 64.7
MS.gene061314.t1 MTR_8g018350 30.636 173 95 7 268 434 15 168 8.80e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061314.t1 AT3G53110 60.093 431 149 7 22 436 54 477 5.68e-178 506
MS.gene061314.t1 AT1G72730 34.834 422 238 12 28 434 7 406 3.61e-67 220
MS.gene061314.t1 AT3G19760 33.750 400 227 10 48 434 26 400 7.74e-64 211
MS.gene061314.t1 AT3G13920 34.704 389 215 11 61 434 30 394 2.71e-62 207
MS.gene061314.t1 AT3G13920 34.704 389 215 11 61 434 40 404 3.85e-62 207
MS.gene061314.t1 AT1G54270 34.447 389 216 11 61 434 40 404 1.25e-60 203
MS.gene061314.t1 AT3G13920 35.027 374 205 10 61 419 40 390 2.02e-60 202
MS.gene061314.t1 AT3G13920 35.027 374 205 10 61 419 44 394 2.40e-60 202
MS.gene061314.t1 AT3G13920 34.190 389 212 11 61 434 40 399 2.29e-59 199
MS.gene061314.t1 AT1G54270 33.933 389 213 11 61 434 40 399 8.84e-58 195
MS.gene061314.t1 AT1G51380 32.338 402 234 11 48 436 13 389 2.05e-57 194
MS.gene061314.t1 AT4G00660 32.653 392 225 11 57 434 128 494 4.93e-57 196
MS.gene061314.t1 AT4G00660 32.653 392 225 11 57 434 128 494 4.93e-57 196
MS.gene061314.t1 AT3G61240 32.668 401 213 12 57 434 121 487 8.62e-54 187
MS.gene061314.t1 AT3G61240 32.668 401 213 12 57 434 121 487 8.62e-54 187
MS.gene061314.t1 AT2G45810 32.678 407 205 15 57 434 151 517 9.65e-53 185
MS.gene061314.t1 AT5G11170 30.516 426 237 15 35 434 23 415 3.22e-49 173
MS.gene061314.t1 AT5G11200 30.516 426 237 15 35 434 23 415 3.22e-49 173
MS.gene061314.t1 AT5G11200 28.387 465 237 16 35 434 23 456 1.54e-43 159
MS.gene061314.t1 AT5G11200 27.593 482 237 16 35 434 23 474 5.94e-42 155
MS.gene061314.t1 AT5G11170 29.609 358 200 10 102 434 2 332 7.55e-41 149
MS.gene061314.t1 AT3G01540 29.793 386 229 11 45 411 144 506 1.13e-39 150
MS.gene061314.t1 AT3G01540 29.793 386 229 11 45 411 144 506 1.16e-39 150
MS.gene061314.t1 AT3G01540 29.793 386 229 11 45 411 144 506 1.16e-39 150
MS.gene061314.t1 AT3G01540 29.793 386 229 11 45 411 144 506 1.16e-39 150
MS.gene061314.t1 AT3G06480 30.871 379 220 11 54 414 432 786 3.30e-39 151
MS.gene061314.t1 AT5G63120 28.307 378 229 10 62 419 167 522 5.90e-39 148
MS.gene061314.t1 AT3G22310 28.321 399 226 13 64 434 120 486 3.01e-37 143
MS.gene061314.t1 AT3G22310 28.321 399 226 13 64 434 120 486 3.42e-37 143
MS.gene061314.t1 AT5G14610 29.380 371 219 11 61 411 170 517 4.75e-37 143
MS.gene061314.t1 AT5G14610 29.380 371 219 11 61 411 162 509 5.68e-37 143
MS.gene061314.t1 AT5G14610 29.380 371 219 11 61 411 162 509 6.04e-37 143
MS.gene061314.t1 AT5G60990 30.025 393 195 14 61 411 10 364 1.06e-36 140
MS.gene061314.t1 AT2G42520 30.184 381 217 10 61 409 160 523 2.59e-36 141
MS.gene061314.t1 AT2G42520 30.184 381 217 10 61 409 160 523 2.59e-36 141
MS.gene061314.t1 AT2G42520 30.184 381 217 10 61 409 160 523 2.59e-36 141
MS.gene061314.t1 AT4G33370 30.159 378 212 10 62 411 99 452 9.01e-36 139
MS.gene061314.t1 AT1G55150 28.895 353 210 8 80 412 118 449 1.26e-35 138
MS.gene061314.t1 AT3G22330 26.969 419 246 13 44 434 88 474 1.27e-35 139
MS.gene061314.t1 AT1G55150 28.895 353 210 8 80 412 143 474 2.05e-35 137
MS.gene061314.t1 AT3G58570 29.134 381 221 10 61 409 147 510 3.27e-35 138
MS.gene061314.t1 AT1G16280 31.500 400 234 13 54 435 52 429 5.18e-35 136
MS.gene061314.t1 AT1G31970 28.708 418 234 12 56 433 112 505 5.32e-35 136
MS.gene061314.t1 AT5G51280 30.854 363 198 10 78 411 163 501 6.06e-35 137
MS.gene061314.t1 AT5G14610 28.933 356 211 10 75 410 44 377 2.04e-34 134
MS.gene061314.t1 AT5G14610 28.933 356 211 10 75 410 44 377 2.04e-34 134
MS.gene061314.t1 AT5G14610 28.686 373 223 11 59 411 228 577 2.14e-34 136
MS.gene061314.t1 AT3G58510 27.763 371 225 8 81 424 171 525 2.26e-34 135
MS.gene061314.t1 AT3G58510 27.763 371 225 8 81 424 171 525 2.26e-34 135
MS.gene061314.t1 AT3G58510 27.763 371 225 8 81 424 171 525 2.26e-34 135
MS.gene061314.t1 AT5G08620 29.730 444 241 17 25 421 33 452 7.11e-34 134
MS.gene061314.t1 AT5G26742 28.406 433 234 14 37 435 87 477 1.89e-33 133
MS.gene061314.t1 AT5G26742 28.406 433 234 14 37 435 87 477 1.89e-33 133
MS.gene061314.t1 AT5G62190 29.600 375 216 13 87 436 122 473 1.95e-33 133
MS.gene061314.t1 AT5G26742 29.050 358 211 9 101 435 47 384 7.57e-33 131
MS.gene061314.t1 AT3G09720 30.398 352 203 10 71 405 152 478 1.22e-32 130
MS.gene061314.t1 AT3G09720 30.398 352 203 10 71 405 152 478 1.22e-32 130
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 193 545 2.07e-32 130
MS.gene061314.t1 AT1G77030 29.288 379 212 15 62 414 30 378 7.51e-32 129
MS.gene061314.t1 AT4G16630 28.718 390 235 10 59 428 166 532 8.51e-32 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT1G20920 28.117 377 223 12 62 414 531 883 1.07e-31 129
MS.gene061314.t1 AT2G47330 26.440 382 242 9 51 413 219 580 2.19e-31 127
MS.gene061314.t1 AT2G33730 28.061 392 227 10 61 416 314 686 2.84e-30 124
MS.gene061314.t1 AT5G08610 30.025 403 226 16 56 425 378 757 1.18e-29 122
MS.gene061314.t1 AT3G06480 30.149 335 202 10 54 370 432 752 1.22e-29 122
MS.gene061314.t1 AT3G18600 28.496 379 230 11 61 419 90 447 1.96e-29 121
MS.gene061314.t1 AT5G63630 28.777 417 243 15 37 421 32 426 5.63e-29 119
MS.gene061314.t1 AT5G63630 28.777 417 243 15 37 421 226 620 1.24e-28 119
MS.gene061314.t1 AT1G51380 31.047 277 164 8 48 311 13 275 2.13e-28 113
MS.gene061314.t1 AT5G14610 28.698 338 206 11 61 378 170 492 2.50e-28 117
MS.gene061314.t1 AT3G02065 28.686 373 223 9 61 411 111 462 3.04e-28 117
MS.gene061314.t1 AT3G02065 28.686 373 223 9 61 411 111 462 3.04e-28 117
MS.gene061314.t1 AT1G12770 25.708 459 224 17 54 429 104 528 4.80e-27 114
MS.gene061314.t1 AT1G12770 25.708 459 224 17 54 429 104 528 4.80e-27 114
MS.gene061314.t1 AT5G54910 27.820 399 235 16 53 425 64 435 5.06e-27 114
MS.gene061314.t1 AT1G28180 29.107 347 199 10 96 418 6 329 5.50e-27 111
MS.gene061314.t1 AT5G63120 27.500 320 200 9 62 361 167 474 1.29e-26 112
MS.gene061314.t1 AT5G63120 27.500 320 200 9 62 361 167 474 1.38e-26 112
MS.gene061314.t1 AT5G63120 27.500 320 200 9 62 361 167 474 1.38e-26 112
MS.gene061314.t1 AT5G63120 27.500 320 200 9 62 361 167 474 1.38e-26 112
MS.gene061314.t1 AT5G65900 28.493 365 221 11 61 405 155 499 2.88e-26 112
MS.gene061314.t1 AT3G02065 27.647 340 206 7 101 418 11 332 5.42e-25 105
MS.gene061314.t1 AT2G40700 25.977 435 221 15 58 405 28 448 5.51e-23 102
MS.gene061314.t1 AT4G09730 24.750 400 240 11 62 425 114 488 1.95e-22 100
MS.gene061314.t1 AT2G07750 28.499 393 217 17 58 412 373 739 2.78e-22 100
MS.gene061314.t1 AT1G63250 28.389 391 220 17 58 412 326 692 5.40e-22 99.4
MS.gene061314.t1 AT1G51380 29.915 234 139 7 48 268 13 234 1.25e-20 92.4
MS.gene061314.t1 AT1G51380 29.915 234 139 7 48 268 13 234 1.49e-20 92.0
MS.gene061314.t1 AT4G34910 26.747 415 197 14 61 400 47 429 3.01e-20 94.0
MS.gene061314.t1 AT4G15850 24.374 439 237 20 67 428 38 458 8.98e-16 79.7
MS.gene061314.t1 AT5G05450 25.773 388 233 15 67 419 23 390 2.44e-14 75.5

Find 109 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATCACTTGTTGATTT+TGG 0.105833 4.2:-51904737 MS.gene061314:CDS
CACTAGAGAGTTAGCTATTC+AGG 0.208975 4.2:-51907030 MS.gene061314:intron
TCAAAGATTGAAGAACATTT+TGG 0.221305 4.2:-51903958 MS.gene061314:CDS
AAAGATTATATATTCGAATT+AGG 0.222892 4.2:-51904818 MS.gene061314:CDS
GTTGGAAGGGCTGGGAGATT+TGG 0.231838 4.2:-51904394 MS.gene061314:CDS
TTAGGACCGTCGAGCAATTT+AGG 0.249173 4.2:+51907446 None:intergenic
ATCGAGGCGGCCACCTATTA+TGG 0.259539 4.2:-51906300 MS.gene061314:CDS
ATTATGGCTCAGGTGGTTAT+TGG 0.271181 4.2:-51906284 MS.gene061314:CDS
CCGTGTACTTTCCCATCTTT+TGG 0.286685 4.2:+51906369 None:intergenic
TGACCATTTGCTCACTTTCA+TGG 0.287401 4.2:+51903983 None:intergenic
CACAATCAGGCTCAGGTTCT+TGG 0.296941 4.2:+51904434 None:intergenic
TTGCACAGGGTTGGAAGGGC+TGG 0.302775 4.2:-51904403 MS.gene061314:CDS
TTGGAAGGGCTGGGAGATTT+GGG 0.308133 4.2:-51904393 MS.gene061314:CDS
GCTCACTTTCATGGCATATC+AGG 0.318809 4.2:+51903992 None:intergenic
TTGCGTTTGTTAGGTCAATT+TGG 0.334175 4.2:-51904494 MS.gene061314:intron
GTTTCATTGTTGATGTCATT+AGG 0.354162 4.2:+51907428 None:intergenic
ATAACCACCTGAGCCATAAT+AGG 0.389402 4.2:+51906287 None:intergenic
GATTTAAAGATGATTCTCTA+AGG 0.399276 4.2:-51905983 MS.gene061314:CDS
TGCAAATACACCTCACAATC+AGG 0.402860 4.2:+51904421 None:intergenic
GCACAGGCACGTAATGGTTC+TGG 0.405343 4.2:-51907145 MS.gene061314:CDS
AAAATTCAATTCAAACTGTC+AGG 0.405889 4.2:-51905943 MS.gene061314:intron
GATTCTTAACCCTCCACATA+AGG 0.417179 4.2:-51907176 MS.gene061314:CDS
TTCATGTAGGAGGTTAACTC+TGG 0.418134 4.2:-51907325 MS.gene061314:intron
TCAATCTTAGCGAGTTCATC+AGG 0.435983 4.2:+51904848 None:intergenic
AGTTTGAAGAGCACCTTGTA+TGG 0.450163 4.2:+51904700 None:intergenic
TGCACAGGGTTGGAAGGGCT+GGG 0.452993 4.2:-51904402 MS.gene061314:CDS
TAATTAATGCTTCGAACGAT+AGG 0.457755 4.2:+51907489 None:intergenic
TACAAGGATTGTTGCGAAGA+AGG 0.471371 4.2:-51904942 MS.gene061314:CDS
AATGTTGCCGCAGATGTGTA+AGG 0.471413 4.2:+51907295 None:intergenic
TTCAAGCTATTAATTGTGCC+AGG 0.471989 4.2:+51906257 None:intergenic
CAGGTGGTTATTGGTACTCC+TGG 0.473085 4.2:-51906275 MS.gene061314:CDS
CATGCTAGGAATTGCAAGTT+CGG 0.473885 4.2:+51906337 None:intergenic
ATCATCAAGGAATTCAAAGA+TGG 0.474651 4.2:-51904662 MS.gene061314:CDS
CTGTCAAGAATTTCGCTACA+AGG 0.476466 4.2:-51904958 MS.gene061314:CDS
CAACGAGGTTCTCCAAAAGA+TGG 0.479543 4.2:-51906381 MS.gene061314:CDS
GGCTGGGAGATTTGGGCGCA+AGG 0.483360 4.2:-51904386 MS.gene061314:CDS
CTGAATAGCTAACTCTCTAG+TGG 0.485079 4.2:+51907031 None:intergenic
CGAAGAGCTTTCCCTCTCTC+CGG 0.485118 4.2:-51907272 MS.gene061314:CDS
GAAGGGACTCTATGTGGAGA+TGG 0.491083 4.2:-51907242 MS.gene061314:CDS
GGCTGATCAAATGCTTGCTC+AGG 0.491796 4.2:-51906183 MS.gene061314:intron
TTGATTGCACAGGCACGTAA+TGG 0.496323 4.2:-51907151 MS.gene061314:CDS
GCGGCCACCTATTATGGCTC+AGG 0.498061 4.2:-51906294 MS.gene061314:CDS
CTTGGCCACGAGTGTACTTG+AGG 0.506845 4.2:+51904452 None:intergenic
TGAATAGCTAACTCTCTAGT+GGG 0.508229 4.2:+51907032 None:intergenic
ATTGTGAGGTGTATTTGCAC+AGG 0.512836 4.2:-51904417 MS.gene061314:CDS
TAGAGTCCCTTCAGTAGCTC+CGG 0.515039 4.2:+51907253 None:intergenic
CCTCTCTCCGGAGCTACTGA+AGG 0.515298 4.2:-51907260 MS.gene061314:CDS
AGCGAAGGCCTCTGCACCGG+AGG 0.517216 4.2:+51907520 None:intergenic
AAGGGACTCTATGTGGAGAT+GGG 0.518643 4.2:-51907241 MS.gene061314:CDS
TAGCATTTACCTCTTCTACA+TGG 0.519872 4.2:+51903932 None:intergenic
TGTATTTGCACAGGGTTGGA+AGG 0.520929 4.2:-51904408 MS.gene061314:CDS
TCGAATTAGGAGAGAATGTA+GGG 0.522425 4.2:-51904805 MS.gene061314:CDS
ACATCCTCCCTCCGGTGCAG+AGG 0.530447 4.2:-51907528 MS.gene061314:CDS
TAATGACATCAACAATGAAA+CGG 0.537170 4.2:-51907426 MS.gene061314:intron
CCAAAAGATGGGAAAGTACA+CGG 0.539369 4.2:-51906369 MS.gene061314:CDS
ACATAAGGATTTGATTGCAC+AGG 0.540312 4.2:-51907161 MS.gene061314:CDS
CGAACTTGCAATTCCTAGCA+TGG 0.543705 4.2:-51906336 MS.gene061314:CDS
ATGCCATGAAAGTGAGCAAA+TGG 0.545233 4.2:-51903986 MS.gene061314:CDS
ATAATAGGTGGCCGCCTCGA+TGG 0.554163 4.2:+51906302 None:intergenic
TACAGCGAAGGCCTCTGCAC+CGG 0.554832 4.2:+51907517 None:intergenic
GTATTTGCACAGGGTTGGAA+GGG 0.557618 4.2:-51904407 MS.gene061314:CDS
AAGGCCTCTGCACCGGAGGG+AGG 0.559222 4.2:+51907524 None:intergenic
TTCGAATTAGGAGAGAATGT+AGG 0.561784 4.2:-51904806 MS.gene061314:CDS
GGGCAAATGCAGAGTGCTTG+AGG 0.564596 4.2:+51907053 None:intergenic
TTGAAGAATCATAAGAAACT+CGG 0.566015 4.2:-51906239 MS.gene061314:CDS
AAACCTTCACATCCTCCCTC+CGG 0.568592 4.2:-51907536 MS.gene061314:CDS
CCTTCAGTAGCTCCGGAGAG+AGG 0.570129 4.2:+51907260 None:intergenic
AACGAGGTTCTCCAAAAGAT+GGG 0.570130 4.2:-51906380 MS.gene061314:CDS
TTGGCCACGAGTGTACTTGA+GGG 0.570896 4.2:+51904453 None:intergenic
CTTCAGTAGCTCCGGAGAGA+GGG 0.573207 4.2:+51907261 None:intergenic
GCACCGGAGGGAGGATGTGA+AGG 0.575110 4.2:+51907533 None:intergenic
ATGTTGCCGCAGATGTGTAA+GGG 0.579048 4.2:+51907296 None:intergenic
ATAGGTGGCCGCCTCGATGG+TGG 0.579697 4.2:+51906305 None:intergenic
TGAAGAATCATAAGAAACTC+GGG 0.579779 4.2:-51906238 MS.gene061314:CDS
TACACCTCACAATCAGGCTC+AGG 0.583588 4.2:+51904427 None:intergenic
GCTACTGAAGGGACTCTATG+TGG 0.586084 4.2:-51907248 MS.gene061314:CDS
CTCTCTCCGGAGCTACTGAA+GGG 0.586509 4.2:-51907259 MS.gene061314:CDS
CAATCAAATCCTTATGTGGA+GGG 0.588286 4.2:+51907167 None:intergenic
TGTGGAGGGTTAAGAATCAT+CGG 0.590578 4.2:+51907181 None:intergenic
GCTGGGAGATTTGGGCGCAA+GGG 0.591750 4.2:-51904385 MS.gene061314:intron
GAGGTGTATTTGCACAGGGT+TGG 0.601522 4.2:-51904412 MS.gene061314:CDS
TCATGTAGGAGGTTAACTCT+GGG 0.601910 4.2:-51907324 MS.gene061314:intron
AGAGAGAGACGCAATCATCA+AGG 0.602090 4.2:-51904675 MS.gene061314:CDS
TATGAGGTTACTTCCATACA+AGG 0.606904 4.2:-51904713 MS.gene061314:CDS
TTGTGAGGTGTATTTGCACA+GGG 0.607003 4.2:-51904416 MS.gene061314:CDS
CGATAGGCTTTCTACAGCGA+AGG 0.609905 4.2:+51907505 None:intergenic
CTGATCCTGATCAAAACCAC+GGG 0.612121 4.2:+51904604 None:intergenic
GCGAAGGCCTCTGCACCGGA+GGG 0.614551 4.2:+51907521 None:intergenic
GCAATCAAATCCTTATGTGG+AGG 0.618196 4.2:+51907166 None:intergenic
TTTACCAAGCACGAAACAAG+TGG 0.620291 4.2:+51907117 None:intergenic
CAAAAGATGGGAAAGTACAC+GGG 0.620858 4.2:-51906368 MS.gene061314:CDS
TGTGCAATCAAATCCTTATG+TGG 0.625550 4.2:+51907163 None:intergenic
TCAACCGACGTTCTTTCCCG+TGG 0.626594 4.2:-51904620 MS.gene061314:CDS
GCCACCTATTATGGCTCAGG+TGG 0.637359 4.2:-51906291 MS.gene061314:CDS
TGGCCACGAGTGTACTTGAG+GGG 0.639967 4.2:+51904454 None:intergenic
TGTTGCCGCAGATGTGTAAG+GGG 0.641199 4.2:+51907297 None:intergenic
AGTTCTCCTAAATTGCTCGA+CGG 0.642807 4.2:-51907452 MS.gene061314:CDS
ACCACCTGAGCCATAATAGG+TGG 0.663209 4.2:+51906290 None:intergenic
CTTCCCCTCAAGTACACTCG+TGG 0.665981 4.2:-51904457 MS.gene061314:CDS
AGGGACTCTATGTGGAGATG+GGG 0.673029 4.2:-51907240 MS.gene061314:CDS
AGAACCTGAGCCTGATTGTG+AGG 0.676199 4.2:-51904431 MS.gene061314:CDS
AGGGTTAAGAATCATCGGCA+AGG 0.681562 4.2:+51907186 None:intergenic
CCTGATCCTGATCAAAACCA+CGG 0.683438 4.2:+51904603 None:intergenic
GAATAGCTAACTCTCTAGTG+GGG 0.713974 4.2:+51907033 None:intergenic
ACAAAACTCCACCATCGAGG+CGG 0.714497 4.2:-51906313 MS.gene061314:CDS
GTACTCCCCTTACACATCTG+CGG 0.724222 4.2:-51907302 MS.gene061314:CDS
TGGACAAAACTCCACCATCG+AGG 0.743095 4.2:-51906316 MS.gene061314:CDS
AAAACCACGGGAAAGAACGT+CGG 0.746447 4.2:+51904616 None:intergenic
GTGTTTGTATTAGAACAACG+AGG 0.784463 4.2:-51906396 MS.gene061314:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGATTATTACAATATAT+TGG + chr4.2:51907218-51907237 None:intergenic 10.0%
!!! ATATATTTTGAAATCAATAT+TGG - chr4.2:51904685-51904704 MS.gene061314:CDS 10.0%
!!! TATATTTTGAAATCAATATT+GGG - chr4.2:51904686-51904705 MS.gene061314:CDS 10.0%
!!! TATATTTTGTAATTCAAATA+TGG - chr4.2:51906061-51906080 MS.gene061314:intron 10.0%
!! AAAAAAAATATGGGAATAAA+AGG + chr4.2:51905828-51905847 None:intergenic 15.0%
!! AAAGATTATATATTCGAATT+AGG - chr4.2:51906654-51906673 MS.gene061314:intron 15.0%
!! AAGAAGAGAAAAAAAAAATA+TGG + chr4.2:51905838-51905857 None:intergenic 15.0%
!! AGAAGAGAAAAAAAAAATAT+GGG + chr4.2:51905837-51905856 None:intergenic 15.0%
!! ATTAAAAGAAAGCAAATAAA+TGG + chr4.2:51905973-51905992 None:intergenic 15.0%
!! GAATCTTTAAATATAAATCA+AGG + chr4.2:51907274-51907293 None:intergenic 15.0%
!! TACAGTTATTAAGTAAATTA+GGG + chr4.2:51905448-51905467 None:intergenic 15.0%
!! TTACAGTTATTAAGTAAATT+AGG + chr4.2:51905449-51905468 None:intergenic 15.0%
!!! ATATTTTAAAGTATGATTTC+AGG - chr4.2:51904940-51904959 MS.gene061314:CDS 15.0%
!!! TATTTTTAACTCACTAATTT+GGG + chr4.2:51904745-51904764 None:intergenic 15.0%
!!! TTATTTTTAACTCACTAATT+TGG + chr4.2:51904746-51904765 None:intergenic 15.0%
!!! TTTTAGAACTTTTTTGATAT+TGG - chr4.2:51905717-51905736 MS.gene061314:intron 15.0%
!! AGAAATTAACTTTAACTCAT+AGG - chr4.2:51904113-51904132 MS.gene061314:intron 20.0%
!! AGTTGTAATCTGAATTATTT+AGG - chr4.2:51906017-51906036 MS.gene061314:intron 20.0%
!! ATAATCATAAGAGTAAACTT+TGG - chr4.2:51907228-51907247 MS.gene061314:CDS 20.0%
!! ATCTTAGTAATTGTAAACAA+TGG - chr4.2:51904967-51904986 MS.gene061314:CDS 20.0%
!! ATTAAGTACAAACAAAATCA+TGG + chr4.2:51904487-51904506 None:intergenic 20.0%
!! ATTGTATTGTTCTTCATAAT+TGG + chr4.2:51904801-51904820 None:intergenic 20.0%
!! GAAATTAACTTTAACTCATA+GGG - chr4.2:51904114-51904133 MS.gene061314:intron 20.0%
!! GTCAACAAAATTTAAAGAAT+AGG + chr4.2:51906376-51906395 None:intergenic 20.0%
!! GTTGTAATCTGAATTATTTA+GGG - chr4.2:51906018-51906037 MS.gene061314:intron 20.0%
!! GTTTAATGATATGAATAAGA+TGG - chr4.2:51906431-51906450 MS.gene061314:intron 20.0%
!!! ATTATATGTTGTTTAACTCT+AGG - chr4.2:51905335-51905354 MS.gene061314:intron 20.0%
!!! TAATATTTTATCTGAATGCA+AGG - chr4.2:51904840-51904859 MS.gene061314:CDS 20.0%
!!! TCTTTACTTTTTAACAGATT+CGG - chr4.2:51905404-51905423 MS.gene061314:intron 20.0%
!!! TGTCTTCTTGAATTTTTAAT+CGG - chr4.2:51906230-51906249 MS.gene061314:CDS 20.0%
! AAAATTCAATTCAAACTGTC+AGG - chr4.2:51905529-51905548 MS.gene061314:intron 25.0%
! AACTAATATGGGAATGTTTA+TGG - chr4.2:51905773-51905792 MS.gene061314:intron 25.0%
! AACTTAATTGAAATCATCAC+CGG + chr4.2:51904883-51904902 None:intergenic 25.0%
! AGAAATTATTGCATCGAATA+AGG - chr4.2:51905613-51905632 MS.gene061314:intron 25.0%
! ATATTGGGAATGTGTATAAA+TGG - chr4.2:51904701-51904720 MS.gene061314:CDS 25.0%
! ATCATGTTATGCAACTAATA+TGG - chr4.2:51905761-51905780 MS.gene061314:intron 25.0%
! GATTTAAAGATGATTCTCTA+AGG - chr4.2:51905489-51905508 MS.gene061314:intron 25.0%
! GCAATAATTTCTTGTATGAA+TGG + chr4.2:51905605-51905624 None:intergenic 25.0%
! TAAGCCAAATAAAATACTGA+TGG + chr4.2:51907327-51907346 None:intergenic 25.0%
! TAATGACATCAACAATGAAA+CGG - chr4.2:51904046-51904065 MS.gene061314:intron 25.0%
! TCATGTTATGCAACTAATAT+GGG - chr4.2:51905762-51905781 MS.gene061314:intron 25.0%
! TTAGTAATTGTAAACAATGG+TGG - chr4.2:51904970-51904989 MS.gene061314:CDS 25.0%
! TTGAAGAATCATAAGAAACT+CGG - chr4.2:51905233-51905252 MS.gene061314:intron 25.0%
! TTTGACATTCATAATTGATG+TGG - chr4.2:51905036-51905055 MS.gene061314:intron 25.0%
!! AAGGATAGCACAAAAATTTT+GGG + chr4.2:51907255-51907274 None:intergenic 25.0%
!! CAGTTGAATGTTAATTTGAA+GGG - chr4.2:51904651-51904670 MS.gene061314:CDS 25.0%
!! TACTCCATCAGTATTTTATT+TGG - chr4.2:51907320-51907339 MS.gene061314:intron 25.0%
!! TCAAAGATTGAAGAACATTT+TGG - chr4.2:51907514-51907533 MS.gene061314:CDS 25.0%
!! TGAATATTTTGTGAAGAAAG+AGG - chr4.2:51906560-51906579 MS.gene061314:intron 25.0%
!! TTTTTCTTTTAGACCATGTT+TGG - chr4.2:51904509-51904528 MS.gene061314:intron 25.0%
!! TTTTTGAATTTTCATGTAGG+AGG - chr4.2:51904137-51904156 MS.gene061314:intron 25.0%
!!! CTTCTTATGCTTTAAAATCA+AGG - chr4.2:51904088-51904107 MS.gene061314:intron 25.0%
!!! GTTATTTCTTGAATTTTGCT+AGG + chr4.2:51904267-51904286 None:intergenic 25.0%
!!! TTGCTTTATTTGATTGAACA+GGG - chr4.2:51907446-51907465 MS.gene061314:CDS 25.0%
!!! TTTGCTTTATTTGATTGAAC+AGG - chr4.2:51907445-51907464 MS.gene061314:CDS 25.0%
AATCCTGAATGTCCATTAAT+TGG + chr4.2:51905654-51905673 None:intergenic 30.0%
AGTACAACAAGCAAACATAA+CGG + chr4.2:51906933-51906952 None:intergenic 30.0%
ATATCATGCATAACATTCTG+AGG + chr4.2:51907386-51907405 None:intergenic 30.0%
ATCATCAAGGAATTCAAAGA+TGG - chr4.2:51906810-51906829 MS.gene061314:intron 30.0%
CAATGCTAAGGAATAATTGT+AGG - chr4.2:51905938-51905957 MS.gene061314:intron 30.0%
CCTTCAAATTAACATTCAAC+TGG + chr4.2:51904653-51904672 None:intergenic 30.0%
GAGTTGTTAAACAACACAAT+TGG + chr4.2:51906265-51906284 None:intergenic 30.0%
GGCATTAACTAATATGTATC+CGG - chr4.2:51904861-51904880 MS.gene061314:CDS 30.0%
GTTTCATTGTTGATGTCATT+AGG + chr4.2:51904047-51904066 None:intergenic 30.0%
TAATTAATGCTTCGAACGAT+AGG + chr4.2:51903986-51904005 None:intergenic 30.0%
TACAGTCTGTGTAGAAAATA+TGG - chr4.2:51904606-51904625 MS.gene061314:CDS 30.0%
TATCATGCATAACATTCTGA+GGG + chr4.2:51907385-51907404 None:intergenic 30.0%
TGAAGAATCATAAGAAACTC+GGG - chr4.2:51905234-51905253 MS.gene061314:intron 30.0%
TGGCTTAAAAGAATCAAATC+AGG + chr4.2:51904563-51904582 None:intergenic 30.0%
TTCTTTCTATTTGCAACACA+TGG - chr4.2:51905864-51905883 MS.gene061314:intron 30.0%
! AAAATACTGATGGAGTACTT+TGG + chr4.2:51907317-51907336 None:intergenic 30.0%
! AGGATAGCACAAAAATTTTG+GGG + chr4.2:51907254-51907273 None:intergenic 30.0%
! ATGTCCATTAATTGGTTCAT+TGG + chr4.2:51905646-51905665 None:intergenic 30.0%
! CAAGGATAGCACAAAAATTT+TGG + chr4.2:51907256-51907275 None:intergenic 30.0%
! CCAGTTGAATGTTAATTTGA+AGG - chr4.2:51904650-51904669 MS.gene061314:CDS 30.0%
! GGGTTTTTGAATTTTCATGT+AGG - chr4.2:51904134-51904153 MS.gene061314:intron 30.0%
!! TGCTTTATTTGATTGAACAG+GGG - chr4.2:51907447-51907466 MS.gene061314:CDS 30.0%
!!! CACAAATCACTTGTTGATTT+TGG - chr4.2:51906735-51906754 MS.gene061314:intron 30.0%
AATTGACCTAACAAACGCAA+TGG + chr4.2:51906978-51906997 None:intergenic 35.0%
ACATAAGGATTTGATTGCAC+AGG - chr4.2:51904311-51904330 MS.gene061314:intron 35.0%
ATGGGAATGTTTATGGCATT+TGG - chr4.2:51905780-51905799 MS.gene061314:intron 35.0%
CAATCAAATCCTTATGTGGA+GGG + chr4.2:51904308-51904327 None:intergenic 35.0%
CATTAAACTCCGAATCATCA+AGG + chr4.2:51906419-51906438 None:intergenic 35.0%
CTATTTGCAACACATGGTTT+GGG - chr4.2:51905870-51905889 MS.gene061314:intron 35.0%
GCATTCAATTGCCATTAGTA+AGG - chr4.2:51905892-51905911 MS.gene061314:intron 35.0%
GTACAACAAGCAAACATAAC+GGG + chr4.2:51906932-51906951 None:intergenic 35.0%
GTGTTTGTATTAGAACAACG+AGG - chr4.2:51905076-51905095 MS.gene061314:intron 35.0%
GTTAAACAACACAATTGGAG+AGG + chr4.2:51906260-51906279 None:intergenic 35.0%
TAGCATTTACCTCTTCTACA+TGG + chr4.2:51907543-51907562 None:intergenic 35.0%
TAGTGAGATGTCCTTACTAA+TGG + chr4.2:51905906-51905925 None:intergenic 35.0%
TATGAATAAGATGGTGACCA+AGG - chr4.2:51906440-51906459 MS.gene061314:intron 35.0%
TATGAGGTTACTTCCATACA+AGG - chr4.2:51906759-51906778 MS.gene061314:intron 35.0%
TCAAGGCTATCAAGCATATT+TGG + chr4.2:51906402-51906421 None:intergenic 35.0%
TCAGTTTGTAATCAGAGCTT+AGG - chr4.2:51906334-51906353 MS.gene061314:CDS 35.0%
TCGAATTAGGAGAGAATGTA+GGG - chr4.2:51906667-51906686 MS.gene061314:intron 35.0%
TCTATTTGCAACACATGGTT+TGG - chr4.2:51905869-51905888 MS.gene061314:intron 35.0%
TGAATAGCTAACTCTCTAGT+GGG + chr4.2:51904443-51904462 None:intergenic 35.0%
TGTAACCACAATCCAGATTA+GGG + chr4.2:51904590-51904609 None:intergenic 35.0%
TGTGCAATCAAATCCTTATG+TGG + chr4.2:51904312-51904331 None:intergenic 35.0%
TTACAAACTGATGCAATCCT+AGG + chr4.2:51906325-51906344 None:intergenic 35.0%
TTCAAGCTATTAATTGTGCC+AGG + chr4.2:51905218-51905237 None:intergenic 35.0%
TTCGAATTAGGAGAGAATGT+AGG - chr4.2:51906666-51906685 MS.gene061314:intron 35.0%
TTGGGAATGTGTATAAATGG+TGG - chr4.2:51904704-51904723 MS.gene061314:CDS 35.0%
TTTAGACCATGTTTGGATTG+TGG - chr4.2:51904516-51904535 MS.gene061314:intron 35.0%
TTTGGAGGGTTAAGAATCAT+CGG + chr4.2:51904389-51904408 None:intergenic 35.0%
! ATGCATGATATTTTCAGCAG+TGG - chr4.2:51907395-51907414 MS.gene061314:intron 35.0%
! ATTGCATCGAATAAGGTATC+CGG - chr4.2:51905620-51905639 MS.gene061314:intron 35.0%
! GAACCAATTAATGGACATTC+AGG - chr4.2:51905648-51905667 MS.gene061314:intron 35.0%
! TTGCGTTTGTTAGGTCAATT+TGG - chr4.2:51906978-51906997 MS.gene061314:intron 35.0%
!! TTTCGAGTTTAAAGTCTCCT+AGG - chr4.2:51906305-51906324 MS.gene061314:CDS 35.0%
!!! ACTTGTTGATTTTGGCTATG+AGG - chr4.2:51906743-51906762 MS.gene061314:intron 35.0%
AAAACACACAGAAAGAGAGG+AGG + chr4.2:51906909-51906928 None:intergenic 40.0%
AAAACCACTTGTTTCGTGCT+TGG - chr4.2:51904351-51904370 MS.gene061314:intron 40.0%
AAAACTTTGTTGCTGCAGGA+TGG - chr4.2:51905467-51905486 MS.gene061314:intron 40.0%
AAACACACAGAAAGAGAGGA+GGG + chr4.2:51906908-51906927 None:intergenic 40.0%
AACGAGGTTCTCCAAAAGAT+GGG - chr4.2:51905092-51905111 MS.gene061314:intron 40.0%
AACTATCTGCTTGTGTTCTG+TGG - chr4.2:51907121-51907140 MS.gene061314:intron 40.0%
ACAATCCAGATTAGGGTTTC+TGG + chr4.2:51904583-51904602 None:intergenic 40.0%
AGAAACCCTAATCTGGATTG+TGG - chr4.2:51904582-51904601 MS.gene061314:intron 40.0%
AGTTCTCCTAAATTGCTCGA+CGG - chr4.2:51904020-51904039 MS.gene061314:intron 40.0%
ATAACCACCTGAGCCATAAT+AGG + chr4.2:51905188-51905207 None:intergenic 40.0%
ATCCTTCCATTGCGTTTGTT+AGG - chr4.2:51906969-51906988 MS.gene061314:intron 40.0%
ATGCCATGAAAGTGAGCAAA+TGG - chr4.2:51907486-51907505 MS.gene061314:CDS 40.0%
ATTATGGCTCAGGTGGTTAT+TGG - chr4.2:51905188-51905207 MS.gene061314:intron 40.0%
ATTGTGAGGTGTATTTGCAC+AGG - chr4.2:51907055-51907074 MS.gene061314:CDS 40.0%
CACAATCCACAATCCAAACA+TGG + chr4.2:51904525-51904544 None:intergenic 40.0%
CACTAGAGAGTTAGCTATTC+AGG - chr4.2:51904442-51904461 MS.gene061314:CDS 40.0%
CAGAAACACAAGTCAATCCT+TGG + chr4.2:51906460-51906479 None:intergenic 40.0%
CATGCTAGGAATTGCAAGTT+CGG + chr4.2:51905138-51905157 None:intergenic 40.0%
CTGAATAGCTAACTCTCTAG+TGG + chr4.2:51904444-51904463 None:intergenic 40.0%
CTGTAAAACTTTGTTGCTGC+AGG - chr4.2:51905463-51905482 MS.gene061314:intron 40.0%
CTGTAACCACAATCCAGATT+AGG + chr4.2:51904591-51904610 None:intergenic 40.0%
CTGTCAAGAATTTCGCTACA+AGG - chr4.2:51906514-51906533 MS.gene061314:intron 40.0%
GAAGACATCACAATCAGATG+TGG + chr4.2:51906217-51906236 None:intergenic 40.0%
GAATAGCTAACTCTCTAGTG+GGG + chr4.2:51904442-51904461 None:intergenic 40.0%
GATTCTTAACCCTCCACATA+AGG - chr4.2:51904296-51904315 MS.gene061314:intron 40.0%
GCAATCAAATCCTTATGTGG+AGG + chr4.2:51904309-51904328 None:intergenic 40.0%
GGAGACTTTAAACTCGAAAC+AGG + chr4.2:51906304-51906323 None:intergenic 40.0%
GGCTATCAAGCATATTTGGA+TGG + chr4.2:51906398-51906417 None:intergenic 40.0%
GGTCATATCTCTTACTCATG+TGG + chr4.2:51907296-51907315 None:intergenic 40.0%
TACAAGGATTGTTGCGAAGA+AGG - chr4.2:51906530-51906549 MS.gene061314:intron 40.0%
TCAATCTTAGCGAGTTCATC+AGG + chr4.2:51906627-51906646 None:intergenic 40.0%
TCATGTAGGAGGTTAACTCT+GGG - chr4.2:51904148-51904167 MS.gene061314:intron 40.0%
TCTTAACCCTCCAAACATGA+AGG - chr4.2:51904394-51904413 MS.gene061314:CDS 40.0%
TGACCATTTGCTCACTTTCA+TGG + chr4.2:51907492-51907511 None:intergenic 40.0%
TGCAAATACACCTCACAATC+AGG + chr4.2:51907054-51907073 None:intergenic 40.0%
TGTGGAGGGTTAAGAATCAT+CGG + chr4.2:51904294-51904313 None:intergenic 40.0%
TTAAACTCGAAACAGGAGCT+GGG + chr4.2:51906297-51906316 None:intergenic 40.0%
TTAAGCCAGAAACCCTAATC+TGG - chr4.2:51904575-51904594 MS.gene061314:intron 40.0%
TTCATGTAGGAGGTTAACTC+TGG - chr4.2:51904147-51904166 MS.gene061314:intron 40.0%
TTGTGAGGTGTATTTGCACA+GGG - chr4.2:51907056-51907075 MS.gene061314:CDS 40.0%
TTTAAACTCGAAACAGGAGC+TGG + chr4.2:51906298-51906317 None:intergenic 40.0%
TTTACCAAGCACGAAACAAG+TGG + chr4.2:51904358-51904377 None:intergenic 40.0%
! CAAAAGATGGGAAAGTACAC+GGG - chr4.2:51905104-51905123 MS.gene061314:intron 40.0%
! CATGTTTGGATTGTGGATTG+TGG - chr4.2:51904523-51904542 MS.gene061314:intron 40.0%
! CCAAAAGATGGGAAAGTACA+CGG - chr4.2:51905103-51905122 MS.gene061314:intron 40.0%
! CCATTAATTGGTTCATTGGC+CGG + chr4.2:51905642-51905661 None:intergenic 40.0%
! GCTTGATAGCCTTGATGATT+CGG - chr4.2:51906407-51906426 MS.gene061314:intron 40.0%
! TTTTGGCATCCATGTAGAAG+AGG - chr4.2:51907531-51907550 MS.gene061314:CDS 40.0%
!! AGTTTGAAGAGCACCTTGTA+TGG + chr4.2:51906775-51906794 None:intergenic 40.0%
!! GAAGATTCTCGTTTTTGACG+AGG - chr4.2:51905268-51905287 MS.gene061314:intron 40.0%
AAAACCACGGGAAAGAACGT+CGG + chr4.2:51906859-51906878 None:intergenic 45.0%
AACACACAGAAAGAGAGGAG+GGG + chr4.2:51906907-51906926 None:intergenic 45.0%
AAGGGACTCTATGTGGAGAT+GGG - chr4.2:51904231-51904250 MS.gene061314:intron 45.0%
AATGTTGCCGCAGATGTGTA+AGG + chr4.2:51904180-51904199 None:intergenic 45.0%
ACAATCAGATGTGGACGTTC+CGG + chr4.2:51906208-51906227 None:intergenic 45.0%
AGAGAGAGACGCAATCATCA+AGG - chr4.2:51906797-51906816 MS.gene061314:intron 45.0%
AGGGTTAAGAATCATCGGCA+AGG + chr4.2:51904289-51904308 None:intergenic 45.0%
ATAAGTCGGGACAATCGTCA+TGG + chr4.2:51906182-51906201 None:intergenic 45.0%
ATGTTGCCGCAGATGTGTAA+GGG + chr4.2:51904179-51904198 None:intergenic 45.0%
CAACGAGGTTCTCCAAAAGA+TGG - chr4.2:51905091-51905110 MS.gene061314:intron 45.0%
CCGGCCAATGAACCAATTAA+TGG - chr4.2:51905639-51905658 MS.gene061314:intron 45.0%
CCTGATCCTGATCAAAACCA+CGG + chr4.2:51906872-51906891 None:intergenic 45.0%
CGAACTTGCAATTCCTAGCA+TGG - chr4.2:51905136-51905155 MS.gene061314:intron 45.0%
CTGATCCTGATCAAAACCAC+GGG + chr4.2:51906871-51906890 None:intergenic 45.0%
GACCTAACAAACGCAATGGA+AGG + chr4.2:51906974-51906993 None:intergenic 45.0%
GATTGTCCCGACTTATCAAC+CGG - chr4.2:51906186-51906205 MS.gene061314:CDS 45.0%
GCAGCGAATTGACAATGCTA+AGG - chr4.2:51905926-51905945 MS.gene061314:intron 45.0%
GCTCACTTTCATGGCATATC+AGG + chr4.2:51907483-51907502 None:intergenic 45.0%
GGGAAAACACACAGAAAGAG+AGG + chr4.2:51906912-51906931 None:intergenic 45.0%
GGTTAAGAATCATCGGCAAG+TGG + chr4.2:51904287-51904306 None:intergenic 45.0%
GTATTTGCACAGGGTTGGAA+GGG - chr4.2:51907065-51907084 MS.gene061314:intron 45.0%
TAAGTCGGGACAATCGTCAT+GGG + chr4.2:51906181-51906200 None:intergenic 45.0%
TGTATTTGCACAGGGTTGGA+AGG - chr4.2:51907064-51907083 MS.gene061314:intron 45.0%
TTAGGACCGTCGAGCAATTT+AGG + chr4.2:51904029-51904048 None:intergenic 45.0%
TTGATTGCACAGGCACGTAA+TGG - chr4.2:51904321-51904340 MS.gene061314:intron 45.0%
! CCGTGTACTTTCCCATCTTT+TGG + chr4.2:51905106-51905125 None:intergenic 45.0%
!! GGATTGACTTGTGTTTCTGC+AGG - chr4.2:51906461-51906480 MS.gene061314:intron 45.0%
!!! TCTTTCCCGTGGTTTTGATC+AGG - chr4.2:51906863-51906882 MS.gene061314:intron 45.0%
AAACCTTCACATCCTCCCTC+CGG - chr4.2:51903936-51903955 MS.gene061314:CDS 50.0%
ACAAAACTCCACCATCGAGG+CGG - chr4.2:51905159-51905178 MS.gene061314:intron 50.0%
ACCACCTGAGCCATAATAGG+TGG + chr4.2:51905185-51905204 None:intergenic 50.0%
AGAACCTGAGCCTGATTGTG+AGG - chr4.2:51907041-51907060 MS.gene061314:CDS 50.0%
AGGGACTCTATGTGGAGATG+GGG - chr4.2:51904232-51904251 MS.gene061314:intron 50.0%
CACAATCAGGCTCAGGTTCT+TGG + chr4.2:51907041-51907060 None:intergenic 50.0%
CAGGTGGTTATTGGTACTCC+TGG - chr4.2:51905197-51905216 MS.gene061314:intron 50.0%
CGATAGGCTTTCTACAGCGA+AGG + chr4.2:51903970-51903989 None:intergenic 50.0%
GAAGGGACTCTATGTGGAGA+TGG - chr4.2:51904230-51904249 MS.gene061314:intron 50.0%
GAGGAGCCTTCATGTTTGGA+GGG + chr4.2:51904403-51904422 None:intergenic 50.0%
GAGGTGTATTTGCACAGGGT+TGG - chr4.2:51907060-51907079 MS.gene061314:intron 50.0%
GCTACTGAAGGGACTCTATG+TGG - chr4.2:51904224-51904243 MS.gene061314:intron 50.0%
GCTTGAGGAGCCTTCATGTT+TGG + chr4.2:51904407-51904426 None:intergenic 50.0%
GGCTGATCAAATGCTTGCTC+AGG - chr4.2:51905289-51905308 MS.gene061314:intron 50.0%
GTACTCCCCTTACACATCTG+CGG - chr4.2:51904170-51904189 MS.gene061314:intron 50.0%
TACACCTCACAATCAGGCTC+AGG + chr4.2:51907048-51907067 None:intergenic 50.0%
TAGAGTCCCTTCAGTAGCTC+CGG + chr4.2:51904222-51904241 None:intergenic 50.0%
TGACTCGTCTTAGAGCCTAG+TGG - chr4.2:51906150-51906169 MS.gene061314:intron 50.0%
TGAGGAGCCTTCATGTTTGG+AGG + chr4.2:51904404-51904423 None:intergenic 50.0%
TGGACAAAACTCCACCATCG+AGG - chr4.2:51905156-51905175 MS.gene061314:intron 50.0%
TGTTGCCGCAGATGTGTAAG+GGG + chr4.2:51904178-51904197 None:intergenic 50.0%
TTGGAAGGGCTGGGAGATTT+GGG - chr4.2:51907079-51907098 MS.gene061314:intron 50.0%
TTGGCCACGAGTGTACTTGA+GGG + chr4.2:51907022-51907041 None:intergenic 50.0%
!! GACGTTCCGGTTGATAAGTC+GGG + chr4.2:51906195-51906214 None:intergenic 50.0%
!! GGACGTTCCGGTTGATAAGT+CGG + chr4.2:51906196-51906215 None:intergenic 50.0%
!! GGTGGAGTTTTGTCCATGCT+AGG + chr4.2:51905152-51905171 None:intergenic 50.0%
!!! CCGTGGTTTTGATCAGGATC+AGG - chr4.2:51906869-51906888 MS.gene061314:intron 50.0%
ACTCGAAACAGGAGCTGGGA+TGG + chr4.2:51906293-51906312 None:intergenic 55.0%
ATAATAGGTGGCCGCCTCGA+TGG + chr4.2:51905173-51905192 None:intergenic 55.0%
ATCGAGGCGGCCACCTATTA+TGG - chr4.2:51905172-51905191 MS.gene061314:intron 55.0%
CGAAGAGCTTTCCCTCTCTC+CGG - chr4.2:51904200-51904219 MS.gene061314:intron 55.0%
CTCTCTCCGGAGCTACTGAA+GGG - chr4.2:51904213-51904232 MS.gene061314:intron 55.0%
CTTCAGTAGCTCCGGAGAGA+GGG + chr4.2:51904214-51904233 None:intergenic 55.0%
CTTCCCCTCAAGTACACTCG+TGG - chr4.2:51907015-51907034 MS.gene061314:intron 55.0%
CTTGGCCACGAGTGTACTTG+AGG + chr4.2:51907023-51907042 None:intergenic 55.0%
GCACAGGCACGTAATGGTTC+TGG - chr4.2:51904327-51904346 MS.gene061314:intron 55.0%
GCCACCTATTATGGCTCAGG+TGG - chr4.2:51905181-51905200 MS.gene061314:intron 55.0%
GTTGGAAGGGCTGGGAGATT+TGG - chr4.2:51907078-51907097 MS.gene061314:intron 55.0%
TCAACCGACGTTCTTTCCCG+TGG - chr4.2:51906852-51906871 MS.gene061314:intron 55.0%
TGGCCACGAGTGTACTTGAG+GGG + chr4.2:51907021-51907040 None:intergenic 55.0%
! GGGCAAATGCAGAGTGCTTG+AGG + chr4.2:51904422-51904441 None:intergenic 55.0%
! TCGTCATGGGCTAAGCCACT+AGG + chr4.2:51906168-51906187 None:intergenic 55.0%
CCTCTCTCCGGAGCTACTGA+AGG - chr4.2:51904212-51904231 MS.gene061314:intron 60.0%
CCTTCAGTAGCTCCGGAGAG+AGG + chr4.2:51904215-51904234 None:intergenic 60.0%
CGAAACAGGAGCTGGGATGG+TGG + chr4.2:51906290-51906309 None:intergenic 60.0%
GCGGCCACCTATTATGGCTC+AGG - chr4.2:51905178-51905197 MS.gene061314:intron 60.0%
GCTGGGAGATTTGGGCGCAA+GGG - chr4.2:51907087-51907106 MS.gene061314:intron 60.0%
TACAGCGAAGGCCTCTGCAC+CGG + chr4.2:51903958-51903977 None:intergenic 60.0%
TGCACAGGGTTGGAAGGGCT+GGG - chr4.2:51907070-51907089 MS.gene061314:intron 60.0%
TTGCACAGGGTTGGAAGGGC+TGG - chr4.2:51907069-51907088 MS.gene061314:intron 60.0%
ACATCCTCCCTCCGGTGCAG+AGG - chr4.2:51903944-51903963 MS.gene061314:CDS 65.0%
ATAGGTGGCCGCCTCGATGG+TGG + chr4.2:51905170-51905189 None:intergenic 65.0%
GCACCGGAGGGAGGATGTGA+AGG + chr4.2:51903942-51903961 None:intergenic 65.0%
GGCTGGGAGATTTGGGCGCA+AGG - chr4.2:51907086-51907105 MS.gene061314:intron 65.0%
AAGGCCTCTGCACCGGAGGG+AGG + chr4.2:51903951-51903970 None:intergenic 70.0%
AGCGAAGGCCTCTGCACCGG+AGG + chr4.2:51903955-51903974 None:intergenic 70.0%
GCGAAGGCCTCTGCACCGGA+GGG + chr4.2:51903954-51903973 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 51903927 51907567 51903927 ID=MS.gene061314
chr4.2 mRNA 51903927 51907567 51903927 ID=MS.gene061314.t1;Parent=MS.gene061314
chr4.2 exon 51907427 51907567 51907427 ID=MS.gene061314.t1.exon1;Parent=MS.gene061314.t1
chr4.2 CDS 51907427 51907567 51907427 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51907129 51907338 51907129 ID=MS.gene061314.t1.exon2;Parent=MS.gene061314.t1
chr4.2 CDS 51907129 51907338 51907129 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51907031 51907078 51907031 ID=MS.gene061314.t1.exon3;Parent=MS.gene061314.t1
chr4.2 CDS 51907031 51907078 51907031 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51906184 51906405 51906184 ID=MS.gene061314.t1.exon4;Parent=MS.gene061314.t1
chr4.2 CDS 51906184 51906405 51906184 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51905944 51906009 51905944 ID=MS.gene061314.t1.exon5;Parent=MS.gene061314.t1
chr4.2 CDS 51905944 51906009 51905944 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51904604 51905011 51904604 ID=MS.gene061314.t1.exon6;Parent=MS.gene061314.t1
chr4.2 CDS 51904604 51905011 51904604 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51904386 51904503 51904386 ID=MS.gene061314.t1.exon7;Parent=MS.gene061314.t1
chr4.2 CDS 51904386 51904503 51904386 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
chr4.2 exon 51903927 51904027 51903927 ID=MS.gene061314.t1.exon8;Parent=MS.gene061314.t1
chr4.2 CDS 51903927 51904027 51903927 ID=cds.MS.gene061314.t1;Parent=MS.gene061314.t1
Gene Sequence

>MS.gene061314

ATGGCGGAAAAACCTTCACATCCTCCCTCCGGTGCAGAGGCCTTCGCTGTAGAAAGCCTATCGTTCGAAGCATTAATTATCGATGAGAACAAAAGTTCTCCTAAATTGCTCGACGGTCCTAATGACATCAACAATGAAACGGTTCGATATTAATCTTGTTTCTTCTTATGCTTTAAAATCAAGGCAAGAAATTAACTTTAACTCATAGGGTTTTTGAATTTTCATGTAGGAGGTTAACTCTGGGTACTCCCCTTACACATCTGCGGCAACATTCGAAGAGCTTTCCCTCTCTCCGGAGCTACTGAAGGGACTCTATGTGGAGATGGGGTTTAAAAAGCCTAGCAAAATTCAAGAAATAACCTTGCCGATGATTCTTAACCCTCCACATAAGGATTTGATTGCACAGGCACGTAATGGTTCTGGTAAAACCACTTGTTTCGTGCTTGGTAAAACCACTTGCCGATGATTCTTAACCCTCCAAACATGAAGGCTCCTCAAGCACTCTGCATTTGCCCCACTAGAGAGTTAGCTATTCAGGTTATTTGCTTTTCTTTTTGCCATGATTTTGTTTGTACTTAATTGTTTTTCTTTTAGACCATGTTTGGATTGTGGATTGTGGTTTCATAATGAATACCTGATTTGATTCTTTTAAGCCAGAAACCCTAATCTGGATTGTGGTTACAGTCTGTGTAGAAAATATGGACACAATTGTTAACTTTTAAGCCAGTTGAATGTTAATTTGAAGGGCACATTATAATATATATTTTGAAATCAATATTGGGAATGTGTATAAATGGTGGTCTTGAAGTTTACTTCCCAAATTAGTGAGTTAAAAATAAATTTTGAAATCTGATTTCAGTAATATCTGAATCCAATTATGAAGAACAATACAATCATTTTTTTTTTTTATGTGTAATATTTTATCTGAATGCAAGGCATTAACTAATATGTATCCGGTGATGATTTCAATTAAGTTGTCATGAGTCATGCTAGTCAAGAATCACTACTTGTCAATATTTTAAAGTATGATTTCAGGATTAATCTTAGTAATTGTAAACAATGGTGGAGATTTAAACTACTTAATTAAGTGCTTATTTGATTTGATTAAATTTGACATTCATAATTGATGTGGTGTAAATGTTCATTGTTGTGTTTGTATTAGAACAACGAGGTTCTCCAAAAGATGGGAAAGTACACGGGCATTAGTTCCGAACTTGCAATTCCTAGCATGGACAAAACTCCACCATCGAGGCGGCCACCTATTATGGCTCAGGTGGTTATTGGTACTCCTGGCACAATTAATAGCTTGAAGAATCATAAGAAACTCGGGTTGACGAGATTGAAGATTCTCGTTTTTGACGAGGCTGATCAAATGCTTGCTCAGGTAAACTTGAACTTGCTAATCTTGATTATATGTTGTTTAACTCTAGGCTCACAATTCGCATACTAGTACAACTCTATCATGTGTTTTTTCTTTTCTTTACTTTTTAACAGATTCGGTTGCTTAAATATGATGTGCCCTAATTTACTTAATAACTGTAAAACTTTGTTGCTGCAGGATGGATTTAAAGATGATTCTCTAAGGATAATAAAAGAAATAGAAAAATTCAATTCAAACTGTCAGGTTTGTTGATAGATCTTTCTCTTGCTCTGTACACTTGCATGTATAGCATTTCCATTCATACAAGAAATTATTGCATCGAATAAGGTATCCGGCCAATGAACCAATTAATGGACATTCAGGATTAATATAATTTCGCGTTGCTTATGTTTATTTGTTTACATCAAATTTTTAGAACTTTTTTGATATTGGAATCTTCATTTGAAGCTTACTATCATGTTATGCAACTAATATGGGAATGTTTATGGCATTTGGAATTTATTTGAAGAAGCTCACACCTTTTATTCCCATATTTTTTTTTTCTCTTCTTTTTTTTTTCTTTCTATTTGCAACACATGGTTTGGGCATTCAATTGCCATTAGTAAGGACATCTCACTAGCAGCGAATTGACAATGCTAAGGAATAATTGTAGGATGTATTAGCCATTTATTTGCTTTCTTTTAATTTATGATTCGATTTTAAATTTTTTAGTTGTAATCTGAATTATTTAGGGTTTATTGAATCTTGAAGTGCTATATTTTGTAATTCAAATATGGAATAAGAAAGTTAAATTTTATTTCTAGTTTGTAAATCAATAGAAATTCTTTTCTCTTTAGTTATTGTGACTCGTCTTAGAGCCTAGTGGCTTAGCCCATGACGATTGTCCCGACTTATCAACCGGAACGTCCACATCTGATTGTGATGTCTTCTTGAATTTTTAATCGGACATCCTCTCCAATTGTGTTGTTTAACAACTCTTCCACCATCCCAGCTCCTGTTTCGAGTTTAAAGTCTCCTAGGATTGCATCAGTTTGTAATCAGAGCTTAGGTTAAAATAAGCTGTTGCCTATTCTTTAAATTTTGTTGACCATCCAAATATGCTTGATAGCCTTGATGATTCGGAGTTTAATGATATGAATAAGATGGTGACCAAGGATTGACTTGTGTTTCTGCAGGTTCTTCTGTTTTCTGCTACATTTGATGACACTGTCAAGAATTTCGCTACAAGGATTGTTGCGAAGAAGGAGTATAATGAATATTTTGTGAAGAAAGAGGAGCTATCTTTAGAAGCAGTGAAGCAGTACAAAGTGCATTGTCCTGATGAACTCGCTAAGATTGAAGTGATTAAAGATTATATATTCGAATTAGGAGAGAATGTAGGGCAAACTATTATATTTGTGCGCACTAGAAATAGTGCAAAAATGTTGCACAAATCACTTGTTGATTTTGGCTATGAGGTTACTTCCATACAAGGTGCTCTTCAAACTGAAGAGAGAGACGCAATCATCAAGGAATTCAAAGATGGTTTGACTCAAGTTCTTATCTCAACCGACGTTCTTTCCCGTGGTTTTGATCAGGATCAGGTGCTTTCTCTCTCCCCTCCTCTCTTTCTGTGTGTTTTCCCGTTATGTTTGCTTGTTGTACTCATTTGTGTTTCTTGCATCCTTCCATTGCGTTTGTTAGGTCAATTTGGTTATCAACTATGATCTTCCCCTCAAGTACACTCGTGGCCAAGAACCTGAGCCTGATTGTGAGGTGTATTTGCACAGGGTTGGAAGGGCTGGGAGATTTGGGCGCAAGGGTAAGTTTTGAAAACTATCTGCTTGTGTTCTGTGGCTCATTTAAAATTCAGCATGTTTATGCAAAAGATTATTTAACTCACATAGCAGTATTTTATTCATCTCTTTCCAATATATTGTAATAATCATAAGAGTAAACTTTGGCCCCAAAATTTTTGTGCTATCCTTGATTTATATTTAAAGATTCCACATGAGTAAGAGATATGACCAAAGTACTCCATCAGTATTTTATTTGGCTTAGATTTTAACATATTTGAAGTCAATTTGTAATTTCACCCTCAGAATGTTATGCATGATATTTTCAGCAGTGGTGTAGTAACAAGTTTCATTGTTTTAAATTTGCTTTATTTGATTGAACAGGGGCTGTATTTAACCTGATATGCCATGAAAGTGAGCAAATGGTCATGTCAAAGATTGAAGAACATTTTGGCATCCATGTAGAAGAGGTAAATGCTACTTGA
Protein sequence

>MS.gene061314.t1

MAEKPSHPPSGAEAFAVESLSFEALIIDENKSSPKLLDGPNDINNETEVNSGYSPYTSAATFEELSLSPELLKGLYVEMGFKKPSKIQEITLPMILNPPHKDLIAQARNGSGKTTCFAPQALCICPTRELAIQNNEVLQKMGKYTGISSELAIPSMDKTPPSRRPPIMAQVVIGTPGTINSLKNHKKLGLTRLKILVFDEADQMLAQDGFKDDSLRIIKEIEKFNSNCQVLLFSATFDDTVKNFATRIVAKKEYNEYFVKKEELSLEAVKQYKVHCPDELAKIEVIKDYIFELGENVGQTIIFVRTRNSAKMLHKSLVDFGYEVTSIQGALQTEERDAIIKEFKDGLTQVLISTDVLSRGFDQDQVNLVINYDLPLKYTRGQEPEPDCEVYLHRVGRAGRFGRKGAVFNLICHESEQMVMSKIEEHFGIHVEEVNAT