AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85235

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85235.t1 MTR_2g099620 99.758 413 1 0 1 413 1 413 0.0 850
MS.gene85235.t1 MTR_8g018400 94.189 413 24 0 1 413 1 413 0.0 808
MS.gene85235.t1 MTR_2g099620 98.734 395 5 0 19 413 3 397 0.0 803
MS.gene85235.t1 MTR_2g039960 92.978 413 29 0 1 413 1 413 0.0 800
MS.gene85235.t1 MTR_4g125970 91.525 413 35 0 1 413 18 430 0.0 781
MS.gene85235.t1 MTR_4g125970 91.525 413 35 0 1 413 1 413 0.0 781
MS.gene85235.t1 MTR_2g438110 64.611 373 132 0 41 413 34 406 0.0 517
MS.gene85235.t1 MTR_1g046570 61.168 394 116 5 56 413 6 398 4.28e-164 467
MS.gene85235.t1 MTR_8g018370 65.607 346 92 4 71 413 3 324 3.80e-152 433
MS.gene85235.t1 MTR_7g076800 40.984 366 212 4 42 406 142 504 9.22e-89 278
MS.gene85235.t1 MTR_7g076820 41.257 366 211 4 42 406 151 513 1.11e-88 278
MS.gene85235.t1 MTR_7g076830 40.984 366 212 4 42 406 144 506 1.34e-88 278
MS.gene85235.t1 MTR_3g107420 39.691 388 227 7 28 409 33 419 8.19e-79 250
MS.gene85235.t1 MTR_1g012420 38.660 388 231 7 28 409 33 419 3.20e-78 248
MS.gene85235.t1 MTR_4g010565 39.370 381 224 7 35 409 117 496 4.31e-76 245
MS.gene85235.t1 MTR_3g467550 35.467 375 220 5 40 396 116 486 1.96e-67 224
MS.gene85235.t1 MTR_1g061970 36.341 399 214 13 41 405 80 472 1.71e-65 217
MS.gene85235.t1 MTR_3g467550 35.326 368 216 5 47 396 92 455 1.77e-65 218
MS.gene85235.t1 MTR_8g018350 63.905 169 47 5 252 413 15 176 3.02e-65 206
MS.gene85235.t1 MTR_2g100230 35.068 365 223 3 40 394 95 455 6.09e-65 216
MS.gene85235.t1 MTR_4g049910 35.417 384 222 9 41 405 55 431 6.80e-65 214
MS.gene85235.t1 MTR_0915s0010 39.437 284 168 4 54 336 2 282 3.23e-62 202
MS.gene85235.t1 MTR_4g134170 34.301 379 217 7 47 397 293 667 4.54e-62 213
MS.gene85235.t1 MTR_7g109720 34.530 362 230 4 40 394 231 592 6.71e-62 213
MS.gene85235.t1 MTR_6g005320 34.748 377 216 7 47 397 255 627 1.73e-61 210
MS.gene85235.t1 MTR_3g449250 34.865 370 230 5 45 406 94 460 5.86e-61 207
MS.gene85235.t1 MTR_3g449250 32.452 416 258 6 11 406 48 460 1.01e-60 206
MS.gene85235.t1 MTR_8g018330 58.333 168 59 4 252 413 15 177 4.69e-60 193
MS.gene85235.t1 MTR_6g082080 33.065 372 245 3 40 408 9 379 8.38e-60 201
MS.gene85235.t1 MTR_3g083690 34.190 389 233 7 41 407 145 532 1.28e-59 204
MS.gene85235.t1 MTR_7g056830 33.065 372 245 3 40 408 9 379 1.89e-59 200
MS.gene85235.t1 MTR_4g105100 33.773 379 241 4 24 396 189 563 2.02e-59 204
MS.gene85235.t1 MTR_5g090120 34.043 376 235 6 45 411 85 456 4.62e-59 202
MS.gene85235.t1 MTR_2g007000 34.286 385 237 6 40 412 503 883 1.19e-56 201
MS.gene85235.t1 MTR_4g079320 32.976 373 240 5 42 407 20 389 2.21e-56 198
MS.gene85235.t1 MTR_2g032630 33.978 362 232 4 41 396 496 856 7.34e-56 199
MS.gene85235.t1 MTR_2g066050 34.167 360 232 2 41 395 53 412 1.29e-55 191
MS.gene85235.t1 MTR_4g114120 33.418 392 232 7 42 407 141 529 4.15e-55 193
MS.gene85235.t1 MTR_1g054630 33.165 395 230 9 40 407 153 540 8.28e-55 191
MS.gene85235.t1 MTR_3g467550 34.524 336 198 5 40 357 116 447 9.28e-55 188
MS.gene85235.t1 MTR_1g026830 30.513 390 252 8 40 413 146 532 8.96e-54 188
MS.gene85235.t1 MTR_1g095810 30.513 390 252 8 40 413 144 530 4.78e-53 186
MS.gene85235.t1 MTR_6g005550 31.316 380 250 5 41 412 165 541 7.78e-52 185
MS.gene85235.t1 MTR_3g049990 32.812 384 239 7 27 396 135 513 6.05e-51 183
MS.gene85235.t1 MTR_5g027840 33.803 355 221 5 42 384 105 457 1.87e-50 178
MS.gene85235.t1 MTR_6g033070 31.978 369 229 7 41 394 140 501 6.52e-48 171
MS.gene85235.t1 MTR_2g041140 31.593 383 246 7 27 396 124 503 2.62e-46 170
MS.gene85235.t1 MTR_2g055850 31.522 368 229 12 40 391 90 450 4.88e-43 158
MS.gene85235.t1 MTR_7g100820 28.718 390 253 6 34 412 130 505 1.83e-42 158
MS.gene85235.t1 MTR_4g112340 30.726 358 226 10 50 391 106 457 2.36e-42 157
MS.gene85235.t1 MTR_6g056080 31.054 351 215 6 45 380 115 453 2.82e-42 158
MS.gene85235.t1 MTR_4g105100 31.595 326 213 4 24 343 189 510 2.45e-40 150
MS.gene85235.t1 MTR_2g063080 26.173 405 234 7 40 382 108 509 1.34e-39 149
MS.gene85235.t1 MTR_8g027780 29.167 384 245 10 42 406 107 482 1.15e-38 147
MS.gene85235.t1 MTR_3g071620 29.396 364 229 7 47 383 22 384 1.83e-38 146
MS.gene85235.t1 MTR_1g102410 28.926 363 223 5 49 380 52 410 1.45e-37 144
MS.gene85235.t1 MTR_8g027775 28.646 384 247 9 42 406 103 478 2.08e-37 143
MS.gene85235.t1 MTR_7g057250 26.077 441 236 10 41 399 163 595 5.16e-37 143
MS.gene85235.t1 MTR_8g027770 28.385 384 248 8 42 406 202 577 2.74e-36 140
MS.gene85235.t1 MTR_8g097000 27.344 384 257 7 42 406 289 669 9.90e-35 137
MS.gene85235.t1 MTR_3g107420 41.463 205 116 4 35 235 40 244 2.38e-34 130
MS.gene85235.t1 MTR_1g083130 26.406 409 246 8 41 397 26 431 7.07e-34 133
MS.gene85235.t1 MTR_6g039840 28.312 385 238 11 41 396 110 485 2.85e-33 132
MS.gene85235.t1 MTR_4g068990 28.947 342 224 10 68 395 102 438 3.81e-33 132
MS.gene85235.t1 MTR_5g092560 27.062 388 261 9 46 413 81 466 2.70e-32 129
MS.gene85235.t1 MTR_4g112320 30.605 281 182 8 117 391 2 275 8.29e-32 125
MS.gene85235.t1 MTR_8g027780 29.518 332 211 10 42 352 107 436 1.97e-28 116
MS.gene85235.t1 MTR_5g069330 26.478 389 266 7 36 413 2 381 4.08e-28 115
MS.gene85235.t1 MTR_5g069330 26.494 385 263 7 40 413 2 377 6.48e-28 114
MS.gene85235.t1 MTR_3g118120 26.933 401 239 13 24 383 120 507 1.85e-25 108
MS.gene85235.t1 MTR_4g131750 25.700 393 237 13 49 399 90 469 2.07e-25 108
MS.gene85235.t1 MTR_6g039840 29.085 306 188 9 110 395 12 308 4.60e-25 107
MS.gene85235.t1 MTR_0427s0010 32.035 231 107 7 198 405 3 206 9.17e-25 101
MS.gene85235.t1 MTR_4g077370 36.126 191 119 1 36 223 245 435 7.18e-24 103
MS.gene85235.t1 MTR_4g077370 35.079 191 96 4 41 223 2 172 3.61e-18 86.7
MS.gene85235.t1 MTR_5g077690 22.872 376 225 6 79 389 71 446 5.61e-22 98.2
MS.gene85235.t1 MTR_1g022305 25.588 340 181 11 63 395 26 300 1.13e-20 94.0
MS.gene85235.t1 MTR_4g068990 29.464 224 145 6 181 395 8 227 4.42e-20 92.8
MS.gene85235.t1 MTR_5g092560 28.112 249 162 6 181 413 8 255 7.87e-19 89.0
MS.gene85235.t1 MTR_8g032230 36.429 140 82 2 40 175 9 145 9.57e-19 83.6
MS.gene85235.t1 MTR_4g459350 31.285 179 114 4 41 210 41 219 2.22e-18 85.1
MS.gene85235.t1 MTR_4g059460 31.285 179 114 4 41 210 44 222 1.22e-17 85.5
MS.gene85235.t1 MTR_4g059460 35.106 94 61 0 310 403 392 485 1.09e-13 73.2
MS.gene85235.t1 MTR_8g042690 23.238 383 264 13 41 404 18 389 2.07e-17 84.3
MS.gene85235.t1 MTR_2g043360 30.726 179 115 4 41 210 41 219 3.87e-17 84.0
MS.gene85235.t1 MTR_2g043360 35.052 97 62 1 310 406 389 484 3.07e-14 75.1
MS.gene85235.t1 MTR_2g043360 35.052 97 62 1 310 406 271 366 2.79e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85235.t1 AT1G54270 92.995 414 26 2 1 413 1 412 0.0 792
MS.gene85235.t1 AT3G13920 92.512 414 28 2 1 413 1 412 0.0 788
MS.gene85235.t1 AT1G54270 91.787 414 26 3 1 413 1 407 0.0 775
MS.gene85235.t1 AT3G13920 91.304 414 28 3 1 413 1 407 0.0 770
MS.gene85235.t1 AT1G72730 89.372 414 43 1 1 413 1 414 0.0 769
MS.gene85235.t1 AT3G13920 94.330 388 21 1 27 413 15 402 0.0 755
MS.gene85235.t1 AT3G13920 92.366 393 27 2 1 392 1 391 0.0 746
MS.gene85235.t1 AT3G13920 92.366 393 27 2 1 392 5 395 0.0 745
MS.gene85235.t1 AT3G19760 62.725 389 138 1 25 413 27 408 0.0 511
MS.gene85235.t1 AT1G51380 58.108 370 155 0 40 409 22 391 1.28e-162 462
MS.gene85235.t1 AT1G51380 58.103 253 106 0 40 292 22 274 7.50e-108 319
MS.gene85235.t1 AT3G61240 40.650 369 215 4 42 409 126 491 2.81e-90 281
MS.gene85235.t1 AT3G61240 40.650 369 215 4 42 409 126 491 2.81e-90 281
MS.gene85235.t1 AT2G45810 40.872 367 213 4 42 407 156 519 1.21e-88 278
MS.gene85235.t1 AT4G00660 39.837 369 218 4 42 409 133 498 1.29e-88 278
MS.gene85235.t1 AT4G00660 39.837 369 218 4 42 409 133 498 1.29e-88 278
MS.gene85235.t1 AT1G51380 55.660 212 94 0 40 251 22 233 2.05e-82 255
MS.gene85235.t1 AT1G51380 55.660 212 94 0 40 251 22 233 3.86e-82 254
MS.gene85235.t1 AT5G11170 38.660 388 231 7 28 409 33 419 2.14e-77 246
MS.gene85235.t1 AT5G11200 38.660 388 231 7 28 409 33 419 2.14e-77 246
MS.gene85235.t1 AT5G11170 39.169 337 199 6 78 409 1 336 1.04e-69 224
MS.gene85235.t1 AT5G11200 34.965 429 231 8 28 409 33 460 6.25e-69 225
MS.gene85235.t1 AT3G53110 34.988 403 235 10 26 407 81 477 2.06e-67 222
MS.gene85235.t1 AT1G55150 34.626 361 230 2 40 394 99 459 9.57e-66 218
MS.gene85235.t1 AT1G55150 34.626 361 230 2 40 394 124 484 1.10e-65 218
MS.gene85235.t1 AT5G26742 35.238 420 243 10 9 413 80 485 2.27e-64 219
MS.gene85235.t1 AT5G26742 35.238 420 243 10 9 413 80 485 2.30e-64 219
MS.gene85235.t1 AT5G63120 34.085 399 247 4 24 413 153 544 3.71e-64 216
MS.gene85235.t1 AT5G26742 36.458 384 212 10 45 413 26 392 1.46e-63 216
MS.gene85235.t1 AT2G33730 34.987 383 221 6 41 397 314 694 1.76e-63 217
MS.gene85235.t1 AT3G22310 35.484 372 227 6 45 407 121 488 1.54e-62 212
MS.gene85235.t1 AT3G22310 35.484 372 227 6 45 407 121 488 2.04e-62 212
MS.gene85235.t1 AT3G22330 35.215 372 228 6 45 407 109 476 3.70e-62 211
MS.gene85235.t1 AT2G47330 34.359 390 238 7 37 412 222 607 2.87e-60 209
MS.gene85235.t1 AT1G77030 32.527 372 243 4 42 407 30 399 1.63e-59 207
MS.gene85235.t1 AT3G06480 35.156 384 230 6 41 412 436 812 4.97e-59 208
MS.gene85235.t1 AT1G20920 32.850 414 263 7 9 412 163 571 1.31e-58 205
MS.gene85235.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene85235.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene85235.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene85235.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene85235.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene85235.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene85235.t1 AT3G58570 33.753 397 240 8 33 406 136 532 3.71e-57 198
MS.gene85235.t1 AT5G14610 32.808 381 243 6 41 412 162 538 5.23e-57 198
MS.gene85235.t1 AT5G14610 32.808 381 243 6 41 412 162 538 5.77e-57 198
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene85235.t1 AT5G14610 32.808 381 243 6 41 412 170 546 6.71e-57 198
MS.gene85235.t1 AT5G60990 32.071 396 245 5 36 413 5 394 1.68e-56 192
MS.gene85235.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.15e-56 195
MS.gene85235.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene85235.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene85235.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene85235.t1 AT1G16280 33.158 380 238 5 30 396 43 419 1.46e-55 191
MS.gene85235.t1 AT4G16630 33.867 375 239 4 31 396 155 529 2.07e-54 193
MS.gene85235.t1 AT5G14610 32.153 367 236 6 55 412 45 407 1.95e-53 186
MS.gene85235.t1 AT5G14610 32.153 367 236 6 55 412 45 407 1.95e-53 186
MS.gene85235.t1 AT5G11200 31.991 447 238 11 28 409 33 478 8.39e-53 183
MS.gene85235.t1 AT3G02065 32.603 365 233 5 41 394 111 473 2.88e-52 182
MS.gene85235.t1 AT3G02065 32.603 365 233 5 41 394 111 473 2.88e-52 182
MS.gene85235.t1 AT5G14610 32.249 369 237 6 53 412 242 606 3.46e-52 186
MS.gene85235.t1 AT4G33370 30.848 389 250 8 40 412 97 482 6.58e-52 182
MS.gene85235.t1 AT1G28180 34.694 343 208 6 79 410 12 349 1.45e-51 177
MS.gene85235.t1 AT5G51280 30.591 389 251 7 40 412 146 531 1.62e-51 182
MS.gene85235.t1 AT1G31970 33.075 387 241 6 40 413 116 497 2.38e-51 181
MS.gene85235.t1 AT3G18600 31.649 376 247 6 41 408 90 463 3.07e-49 176
MS.gene85235.t1 AT3G09620 32.447 376 209 7 46 409 402 744 4.80e-49 179
MS.gene85235.t1 AT3G09620 32.447 376 209 7 46 409 403 745 5.20e-49 179
MS.gene85235.t1 AT3G09720 29.921 381 260 4 34 412 138 513 1.56e-48 173
MS.gene85235.t1 AT3G09720 29.921 381 260 4 34 412 138 513 1.56e-48 173
MS.gene85235.t1 AT3G02065 32.628 331 210 5 75 394 8 336 7.56e-47 165
MS.gene85235.t1 AT1G12770 27.363 402 230 6 40 381 110 509 8.93e-47 169
MS.gene85235.t1 AT1G12770 27.363 402 230 6 40 381 110 509 8.93e-47 169
MS.gene85235.t1 AT5G65900 30.667 375 251 6 38 404 152 525 1.41e-45 167
MS.gene85235.t1 AT5G62190 32.181 376 227 9 55 411 111 477 2.49e-45 166
MS.gene85235.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene85235.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene85235.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene85235.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.88e-44 161
MS.gene85235.t1 AT3G06480 33.333 324 197 6 41 352 436 752 1.71e-41 156
MS.gene85235.t1 AT5G05450 30.168 358 225 7 50 383 26 382 5.86e-41 153
MS.gene85235.t1 AT1G71370 30.114 352 230 7 47 383 23 373 3.01e-40 151
MS.gene85235.t1 AT5G14610 31.024 332 216 6 41 363 170 497 3.93e-40 149
MS.gene85235.t1 AT5G63630 29.948 384 242 8 42 406 56 431 9.99e-39 146
MS.gene85235.t1 AT5G08620 30.151 398 235 9 42 412 82 463 1.73e-38 146
MS.gene85235.t1 AT5G08610 30.318 409 237 11 28 406 369 759 1.84e-38 148
MS.gene85235.t1 AT5G63630 29.948 384 242 8 42 406 250 625 1.45e-37 145
MS.gene85235.t1 AT2G07750 27.157 394 248 10 42 407 377 759 3.79e-35 138
MS.gene85235.t1 AT1G63250 26.866 402 248 10 35 406 321 706 1.17e-33 134
MS.gene85235.t1 AT4G34910 26.098 387 229 8 40 372 46 429 3.55e-32 129
MS.gene85235.t1 AT5G54910 27.273 396 263 12 17 396 49 435 1.23e-31 127
MS.gene85235.t1 AT5G19210 27.763 389 231 12 49 399 85 461 1.57e-31 125
MS.gene85235.t1 AT2G40700 25.225 444 251 12 41 403 31 474 3.61e-31 125
MS.gene85235.t1 AT4G09730 26.753 385 246 9 40 396 112 488 4.02e-31 125
MS.gene85235.t1 AT3G06980 28.500 400 239 14 21 383 360 749 5.48e-28 117
MS.gene85235.t1 AT1G71280 27.419 310 189 9 47 345 24 308 2.40e-26 108
MS.gene85235.t1 AT4G15850 25.648 386 211 13 79 394 74 453 4.77e-26 110
MS.gene85235.t1 AT1G71280 27.419 310 189 9 47 345 24 308 1.62e-25 108
MS.gene85235.t1 AT3G16840 32.821 195 107 4 41 211 191 385 1.90e-19 91.3
MS.gene85235.t1 AT3G16840 32.821 195 107 4 41 211 192 386 1.98e-19 91.3
MS.gene85235.t1 AT5G19210 26.032 315 183 11 123 399 2 304 1.98e-17 83.2
MS.gene85235.t1 AT5G19210 26.032 315 183 11 123 399 2 304 1.98e-17 83.2
MS.gene85235.t1 AT1G28180 37.273 110 55 2 79 177 12 118 1.02e-11 62.4

Find 90 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACACTCCAACCTGAATTT+TGG 0.139295 2.4:+4640235 None:intergenic
TCTCATTACCACTGACCTTT+TGG 0.272997 2.4:-4639874 MS.gene85235:CDS
ACATCGAGACCCTTGATAAA+AGG 0.302219 2.4:+4640904 None:intergenic
TGCTGTGCCAGCTCCCTTGT+TGG 0.308505 2.4:+4640775 None:intergenic
TCAGTGCCAGGCTTTGGTTT+TGG 0.312026 2.4:-4640800 MS.gene85235:CDS
GTTGCTGCCACCCAAAATTC+AGG 0.315611 2.4:-4640246 MS.gene85235:CDS
GCTGTGCCAGCTCCCTTGTT+GGG 0.317476 2.4:+4640776 None:intergenic
TTGCATTAAAATGTTTGTTT+TGG 0.318028 2.4:-4640578 MS.gene85235:CDS
CGCTTTACCAGAATTCTAAC+TGG 0.324777 2.4:+4640158 None:intergenic
AAGACAGCGACGTTCTGTTC+TGG 0.339543 2.4:-4640853 MS.gene85235:CDS
TGGTGATTACCTTGGTGTTA+AGG 0.345997 2.4:-4640728 MS.gene85235:CDS
TGATGATATCACGAGTGTTT+TGG 0.362515 2.4:+4639928 None:intergenic
GAATAAGCCAGTTAGAATTC+TGG 0.365648 2.4:-4640165 MS.gene85235:CDS
CTGAGAGGCATATATGCTTA+TGG 0.371983 2.4:-4641546 MS.gene85235:intron
GTATCAAATTGCGAACCTTC+CGG 0.376954 2.4:+4641763 None:intergenic
TTATGGTTTGGTTCAGTGCC+AGG 0.388767 2.4:-4640812 MS.gene85235:CDS
AAAACTATCTCCATCGTATT+GGG 0.393330 2.4:-4639788 MS.gene85235:CDS
CAACAACATGGACACCAGTT+TGG 0.395614 2.4:+4640656 None:intergenic
CTTGTGATCTCCAGAGCCTC+TGG 0.397309 2.4:+4640197 None:intergenic
GCAGCAGCTTGATTATGGTT+TGG 0.397488 2.4:-4640824 MS.gene85235:CDS
TTTGGTTCAGTGCCAGGCTT+TGG 0.398715 2.4:-4640806 MS.gene85235:CDS
GAAAACTATCTCCATCGTAT+TGG 0.406801 2.4:-4639789 MS.gene85235:CDS
ATTGGGCGAAGTGGGCGTTT+TGG 0.409374 2.4:-4639771 MS.gene85235:CDS
CATGTTGTTGTTGGTACTCC+TGG 0.424537 2.4:-4640643 MS.gene85235:CDS
AATGCTTTCACGTGGTTTCA+AGG 0.432515 2.4:-4640542 MS.gene85235:CDS
CCCCTTTGCTGTATTGCAGA+TGG 0.433851 2.4:+4640934 None:intergenic
GCCATCTGCAATACAGCAAA+GGG 0.435434 2.4:-4640935 MS.gene85235:CDS
TTCATGAACTTCATCGTATG+AGG 0.438056 2.4:+4641602 None:intergenic
CGTGATGAATTGACCCTTGA+GGG 0.439342 2.4:-4640137 MS.gene85235:CDS
CATGGGTGGCTGAGACAGTA+TGG 0.446776 2.4:+4639961 None:intergenic
TGATGTTCAGCAAGTGTCTC+TGG 0.448249 2.4:-4639841 MS.gene85235:CDS
CAGCAGGCTCAGTCAGGAAC+TGG 0.451120 2.4:-4640877 MS.gene85235:CDS
TACAATGCCTCCAGAGGCTC+TGG 0.455387 2.4:-4640207 MS.gene85235:CDS
CATCAATACCACGGGCCAAA+AGG 0.460041 2.4:+4639859 None:intergenic
TTTGGCCCCAACAAGGGAGC+TGG 0.463851 2.4:-4640782 MS.gene85235:CDS
AAGGTTCATGCTTGTGTTGG+TGG 0.466235 2.4:-4640709 MS.gene85235:CDS
AGTAGATCAGCAACATTTGA+AGG 0.481306 2.4:+4639654 None:intergenic
TGCTGCGAAGACAGTCTCTT+CGG 0.488354 2.4:-4640606 MS.gene85235:CDS
GTGATTCAGCAGGCTCAGTC+AGG 0.498167 2.4:-4640883 MS.gene85235:CDS
CATCTTAGTGTCAAACTGCT+TGG 0.499196 2.4:+4641741 None:intergenic
CTCCATCGTATTGGGCGAAG+TGG 0.499221 2.4:-4639780 MS.gene85235:CDS
ACTGGTGTCCATGTTGTTGT+TGG 0.499852 2.4:-4640652 MS.gene85235:CDS
GATCAGCGTATTCTCCAAAC+TGG 0.501639 2.4:-4640670 MS.gene85235:CDS
TTCACGTGGTTTCAAGGACC+AGG 0.504125 2.4:-4640536 MS.gene85235:intron
ACACAAGGCGCAAGGTGGAC+TGG 0.505122 2.4:-4640010 MS.gene85235:CDS
GTTAAGGTTCATGCTTGTGT+TGG 0.507288 2.4:-4640712 MS.gene85235:CDS
GTGTGATCTTTATGAGACTC+TGG 0.512969 2.4:-4640060 MS.gene85235:CDS
ATCATGCGTGAATTCCGCTC+TGG 0.527239 2.4:-4639909 MS.gene85235:CDS
GAAGAATTCTTGTCCTTCAG+TGG 0.531706 2.4:+4641626 None:intergenic
GAGAAGGTTATGAGAGCACT+TGG 0.533228 2.4:-4640748 MS.gene85235:CDS
AATTACAGGCTTTCCACTGA+AGG 0.540134 2.4:-4641639 MS.gene85235:intron
CTGTGCCAGCTCCCTTGTTG+GGG 0.543113 2.4:+4640777 None:intergenic
TGCTATCAACTTCGTCACAT+TGG 0.543489 2.4:-4639736 MS.gene85235:CDS
AGCATGTCAAACACACGTCC+AGG 0.544974 2.4:+4640625 None:intergenic
GGGTCTCGATGTGATTCAGC+AGG 0.549184 2.4:-4640893 MS.gene85235:CDS
TTGGGGCCAAAACCAAAGCC+TGG 0.554251 2.4:+4640794 None:intergenic
CAGAGGCTCTGGAGATCACA+AGG 0.564855 2.4:-4640196 MS.gene85235:CDS
CTCAGCCACCCATGGAGACA+TGG 0.565513 2.4:-4639952 MS.gene85235:CDS
TCCATCGTATTGGGCGAAGT+GGG 0.570459 2.4:-4639779 MS.gene85235:CDS
CTGCCACCCAAAATTCAGGT+TGG 0.574116 2.4:-4640242 MS.gene85235:CDS
TTGGTCCATGTCTCCATGGG+TGG 0.574389 2.4:+4639947 None:intergenic
AGTCATGGCAGGTTTAGCTC+CGG 0.585504 2.4:-4641782 None:intergenic
AGCCATCTGCAATACAGCAA+AGG 0.592072 2.4:-4640936 MS.gene85235:CDS
CTCCGCTACAATGCCTCCAG+AGG 0.595154 2.4:-4640213 MS.gene85235:CDS
ATTGGAAGATGCAAGAATGC+TGG 0.599752 2.4:-4639718 MS.gene85235:CDS
GCCCACTTCGCCCAATACGA+TGG 0.601432 2.4:+4639778 None:intergenic
AGAACTGCTTGATACCCTCA+AGG 0.605423 2.4:+4640123 None:intergenic
CAGGAGTACCAACAACAACA+TGG 0.608453 2.4:+4640644 None:intergenic
GTGATCTCCAGAGCCTCTGG+AGG 0.613832 2.4:+4640200 None:intergenic
TGAATGTTGACAAGGAAGAG+TGG 0.615932 2.4:-4640097 MS.gene85235:CDS
TTGCTGAACATCAATACCAC+GGG 0.620577 2.4:+4639851 None:intergenic
GCTGGCACAGCAGATTGAGA+AGG 0.628451 2.4:-4640764 MS.gene85235:CDS
CATACTGTCTCAGCCACCCA+TGG 0.631002 2.4:-4639960 MS.gene85235:CDS
AGCCTCTGGAGGCATTGTAG+CGG 0.640236 2.4:+4640211 None:intergenic
ATGGCAGGTTTAGCTCCGGA+AGG 0.641220 2.4:-4641778 MS.gene85235:CDS
AGAGCACTTGGTGATTACCT+TGG 0.644049 2.4:-4640736 MS.gene85235:CDS
GTTCTATAATGTGATCGTAG+AGG 0.657829 2.4:-4639682 MS.gene85235:CDS
CTTGCTGAACATCAATACCA+CGG 0.663030 2.4:+4639850 None:intergenic
GCGTGATGAATTGACCCTTG+AGG 0.663843 2.4:-4640138 MS.gene85235:CDS
ACCACGGGCCAAAAGGTCAG+TGG 0.665992 2.4:+4639866 None:intergenic
TTGATAGAAAATCTTCTGAG+AGG 0.671045 2.4:-4641561 MS.gene85235:CDS
GAACTGCTTGATACCCTCAA+GGG 0.677590 2.4:+4640124 None:intergenic
TGGCTCACTGACAAGATGCG+AGG 0.683723 2.4:-4639990 MS.gene85235:CDS
TGGTGGCACTAGTGTTCGTG+AGG 0.685653 2.4:-4640692 MS.gene85235:CDS
GTTCTACGTGAATGTTGACA+AGG 0.687466 2.4:-4640105 MS.gene85235:CDS
GAACTCGAGAAGAGCCAGAG+CGG 0.689835 2.4:+4639895 None:intergenic
TGTGAACACAAGGCGCAAGG+TGG 0.709342 2.4:-4640015 MS.gene85235:CDS
AAGCATGAACCTTAACACCA+AGG 0.715621 2.4:+4640719 None:intergenic
GCTGATGAAATGCTTTCACG+TGG 0.720414 2.4:-4640550 MS.gene85235:CDS
CCATCTGCAATACAGCAAAG+GGG 0.725959 2.4:-4640934 MS.gene85235:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAGTTTAAAAATTAATT+GGG - chr2.4:4640023-4640042 MS.gene85235:CDS 10.0%
!! GCAAAAGTTTAAAAATTAAT+TGG - chr2.4:4640022-4640041 MS.gene85235:CDS 15.0%
!!! TAATGCATAATTTTAATTAC+AGG - chr2.4:4639775-4639794 MS.gene85235:CDS 15.0%
!! AAGGATTAATAAATTGTGAT+AGG + chr2.4:4639999-4640018 None:intergenic 20.0%
!! AATAAGTGCTTAAAGAAAAA+AGG + chr2.4:4639924-4639943 None:intergenic 20.0%
!! ACCTTGAGTTAATTAATTAA+TGG + chr2.4:4640136-4640155 None:intergenic 20.0%
!! ATATATACAATCAATTTCGA+GGG - chr2.4:4640219-4640238 MS.gene85235:CDS 20.0%
!! ATTAACTCAAGGTTATATAA+TGG - chr2.4:4640143-4640162 MS.gene85235:CDS 20.0%
!! TTAACTCAAGGTTATATAAT+GGG - chr2.4:4640144-4640163 MS.gene85235:CDS 20.0%
!!! ATGAACATTTATTTTTAGAC+AGG - chr2.4:4640084-4640103 MS.gene85235:CDS 20.0%
!!! ATTTTTAAACTTTTGCCAAA+AGG + chr2.4:4640018-4640037 None:intergenic 20.0%
!!! TATATATCAATGAAAAGCAT+GGG + chr2.4:4640206-4640225 None:intergenic 20.0%
!!! TCACAATTTATTAATCCTTT+TGG - chr2.4:4640000-4640019 MS.gene85235:CDS 20.0%
!!! TGAACATTTATTTTTAGACA+GGG - chr2.4:4640085-4640104 MS.gene85235:CDS 20.0%
!!! TTGCATTAAAATGTTTGTTT+TGG - chr2.4:4640850-4640869 MS.gene85235:CDS 20.0%
! AGATGAATGAATTGTAAGTA+AGG - chr2.4:4639705-4639724 MS.gene85235:CDS 25.0%
! AGGTTAAAACGTAATAAGAA+CGG + chr2.4:4640349-4640368 None:intergenic 25.0%
! GATATATACAATCAATTTCG+AGG - chr2.4:4640218-4640237 MS.gene85235:CDS 25.0%
! GCCATTAATTAATTAACTCA+AGG - chr2.4:4640132-4640151 MS.gene85235:CDS 25.0%
! GTATATATCAATGAAAAGCA+TGG + chr2.4:4640207-4640226 None:intergenic 25.0%
! TAAAACAAGATCAATTCATC+TGG + chr2.4:4641103-4641122 None:intergenic 25.0%
! TATATAGTGACAACTATCAA+GGG + chr2.4:4640181-4640200 None:intergenic 25.0%
! TTTAAAAATTAATTGGGTGC+TGG - chr2.4:4640029-4640048 MS.gene85235:CDS 25.0%
!! ATATATCAATGAAAAGCATG+GGG + chr2.4:4640205-4640224 None:intergenic 25.0%
!! TTGTTTGTTAGAACTGAATT+TGG - chr2.4:4640968-4640987 MS.gene85235:intron 25.0%
!!! AACATTTATTTTTAGACAGG+GGG - chr2.4:4640087-4640106 MS.gene85235:CDS 25.0%
!!! GAACATTTATTTTTAGACAG+GGG - chr2.4:4640086-4640105 MS.gene85235:CDS 25.0%
AAAACTATCTCCATCGTATT+GGG - chr2.4:4641640-4641659 MS.gene85235:intron 30.0%
AAATTGTGATAGGCACTAAT+TGG + chr2.4:4639989-4640008 None:intergenic 30.0%
AATTGTGATAGGCACTAATT+GGG + chr2.4:4639988-4640007 None:intergenic 30.0%
AGGTAAAGGGTTATAGAATA+TGG + chr2.4:4641061-4641080 None:intergenic 30.0%
ATATAGTGACAACTATCAAG+GGG + chr2.4:4640180-4640199 None:intergenic 30.0%
CTACGTGTATAGATGTATTT+AGG + chr2.4:4640369-4640388 None:intergenic 30.0%
CTATATAGTGACAACTATCA+AGG + chr2.4:4640182-4640201 None:intergenic 30.0%
TGTGTTAGATTTCTGATTGT+TGG - chr2.4:4640915-4640934 MS.gene85235:CDS 30.0%
TTGATAGAAAATCTTCTGAG+AGG - chr2.4:4639867-4639886 MS.gene85235:CDS 30.0%
! AAGGAAGTAATGCATGATTT+TGG - chr2.4:4639724-4639743 MS.gene85235:CDS 30.0%
! CATGAAACTTTTGATGCAAT+GGG - chr2.4:4639843-4639862 MS.gene85235:CDS 30.0%
! CTTTTCTTTAATCGCATGTT+CGG - chr2.4:4640251-4640270 MS.gene85235:CDS 30.0%
! GGAATTGTTCCTTTTATCAA+GGG - chr2.4:4640515-4640534 MS.gene85235:intron 30.0%
! TCATGAAACTTTTGATGCAA+TGG - chr2.4:4639842-4639861 MS.gene85235:CDS 30.0%
AACTGAATTTGGTTGGAGTT+GGG - chr2.4:4640938-4640957 MS.gene85235:CDS 35.0%
AATCAGAAATCTAACACACC+TGG + chr2.4:4640913-4640932 None:intergenic 35.0%
AGTAGATCAGCAACATTTGA+AGG + chr2.4:4641777-4641796 None:intergenic 35.0%
AGTAGGTCAAATGAAGGTAA+AGG + chr2.4:4641075-4641094 None:intergenic 35.0%
ATGTTCTGTTCTTGTGCTTA+AGG - chr2.4:4640461-4640480 MS.gene85235:intron 35.0%
CTGATTGTTGGAACTGAATT+TGG - chr2.4:4640927-4640946 MS.gene85235:CDS 35.0%
GAAAACTATCTCCATCGTAT+TGG - chr2.4:4641639-4641658 MS.gene85235:intron 35.0%
GAATAAGCCAGTTAGAATTC+TGG - chr2.4:4641263-4641282 MS.gene85235:intron 35.0%
GTAGGTCAAATGAAGGTAAA+GGG + chr2.4:4641074-4641093 None:intergenic 35.0%
GTTCTATAATGTGATCGTAG+AGG - chr2.4:4641746-4641765 MS.gene85235:CDS 35.0%
TTCATGAACTTCATCGTATG+AGG + chr2.4:4639829-4639848 None:intergenic 35.0%
TTGTTGGAACTGAATTTGGT+TGG - chr2.4:4640931-4640950 MS.gene85235:CDS 35.0%
! AACACTCCAACCTGAATTTT+GGG + chr2.4:4641195-4641214 None:intergenic 35.0%
! ATACGATGGAGATAGTTTTC+AGG + chr2.4:4641639-4641658 None:intergenic 35.0%
! ATTTTGCAGCAGCTTGATTA+TGG - chr2.4:4640599-4640618 MS.gene85235:CDS 35.0%
! GGGAATTGTTCCTTTTATCA+AGG - chr2.4:4640514-4640533 MS.gene85235:intron 35.0%
! TGATGATATCACGAGTGTTT+TGG + chr2.4:4641503-4641522 None:intergenic 35.0%
!!! ATGGAGATAGTTTTCAGGTT+GGG + chr2.4:4641634-4641653 None:intergenic 35.0%
!!! GTGTCATTTTTGTGAACACA+AGG - chr2.4:4641403-4641422 MS.gene85235:intron 35.0%
AAGCATGAACCTTAACACCA+AGG + chr2.4:4640712-4640731 None:intergenic 40.0%
AATGCTTTCACGTGGTTTCA+AGG - chr2.4:4640886-4640905 MS.gene85235:CDS 40.0%
AATTACAGGCTTTCCACTGA+AGG - chr2.4:4639789-4639808 MS.gene85235:CDS 40.0%
ACATCGAGACCCTTGATAAA+AGG + chr2.4:4640527-4640546 None:intergenic 40.0%
ATTGGAAGATGCAAGAATGC+TGG - chr2.4:4641710-4641729 MS.gene85235:intron 40.0%
CAAGAACAGAACATCACACA+TGG + chr2.4:4640455-4640474 None:intergenic 40.0%
CAATTCATCTGGTGAGAAGT+AGG + chr2.4:4641092-4641111 None:intergenic 40.0%
CATCTTAGTGTCAAACTGCT+TGG + chr2.4:4639690-4639709 None:intergenic 40.0%
CGCTTTACCAGAATTCTAAC+TGG + chr2.4:4641273-4641292 None:intergenic 40.0%
CTTGCTGAACATCAATACCA+CGG + chr2.4:4641581-4641600 None:intergenic 40.0%
GAACTGAATTTGGTTGGAGT+TGG - chr2.4:4640937-4640956 MS.gene85235:CDS 40.0%
GAAGAATTCTTGTCCTTCAG+TGG + chr2.4:4639805-4639824 None:intergenic 40.0%
GTATCAAATTGCGAACCTTC+CGG + chr2.4:4639668-4639687 None:intergenic 40.0%
GTGAGAAGTAGGTCAAATGA+AGG + chr2.4:4641081-4641100 None:intergenic 40.0%
GTGTGATCTTTATGAGACTC+TGG - chr2.4:4641368-4641387 MS.gene85235:intron 40.0%
GTTAAGGTTCATGCTTGTGT+TGG - chr2.4:4640716-4640735 MS.gene85235:CDS 40.0%
GTTCTACGTGAATGTTGACA+AGG - chr2.4:4641323-4641342 MS.gene85235:intron 40.0%
TGAATGTTGACAAGGAAGAG+TGG - chr2.4:4641331-4641350 MS.gene85235:intron 40.0%
TGCTATCAACTTCGTCACAT+TGG - chr2.4:4641692-4641711 MS.gene85235:intron 40.0%
TTGCTGAACATCAATACCAC+GGG + chr2.4:4641580-4641599 None:intergenic 40.0%
! CTGAGAGGCATATATGCTTA+TGG - chr2.4:4639882-4639901 MS.gene85235:CDS 40.0%
! GAACACTCCAACCTGAATTT+TGG + chr2.4:4641196-4641215 None:intergenic 40.0%
! TCTCATTACCACTGACCTTT+TGG - chr2.4:4641554-4641573 MS.gene85235:CDS 40.0%
!! TGGTGATTACCTTGGTGTTA+AGG - chr2.4:4640700-4640719 MS.gene85235:CDS 40.0%
!!! AGATAGTTTTCAGGTTGGGT+AGG + chr2.4:4641630-4641649 None:intergenic 40.0%
!!! GATGGAGATAGTTTTCAGGT+TGG + chr2.4:4641635-4641654 None:intergenic 40.0%
AAGGTTCATGCTTGTGTTGG+TGG - chr2.4:4640719-4640738 MS.gene85235:CDS 45.0%
ACTGGTGTCCATGTTGTTGT+TGG - chr2.4:4640776-4640795 MS.gene85235:CDS 45.0%
AGAACTGCTTGATACCCTCA+AGG + chr2.4:4641308-4641327 None:intergenic 45.0%
AGCCATCTGCAATACAGCAA+AGG - chr2.4:4640492-4640511 MS.gene85235:intron 45.0%
CAACAACATGGACACCAGTT+TGG + chr2.4:4640775-4640794 None:intergenic 45.0%
CCATCTGCAATACAGCAAAG+GGG - chr2.4:4640494-4640513 MS.gene85235:intron 45.0%
CGTGATGAATTGACCCTTGA+GGG - chr2.4:4641291-4641310 MS.gene85235:intron 45.0%
GAACTGCTTGATACCCTCAA+GGG + chr2.4:4641307-4641326 None:intergenic 45.0%
GATCAGCGTATTCTCCAAAC+TGG - chr2.4:4640758-4640777 MS.gene85235:CDS 45.0%
GCAGCAGCTTGATTATGGTT+TGG - chr2.4:4640604-4640623 MS.gene85235:CDS 45.0%
GCCATCTGCAATACAGCAAA+GGG - chr2.4:4640493-4640512 MS.gene85235:intron 45.0%
GCTGATGAAATGCTTTCACG+TGG - chr2.4:4640878-4640897 MS.gene85235:CDS 45.0%
TCACACATGGTATGCTAGTG+AGG + chr2.4:4640442-4640461 None:intergenic 45.0%
TGATGTTCAGCAAGTGTCTC+TGG - chr2.4:4641587-4641606 MS.gene85235:CDS 45.0%
! ACTCCAACCTGAATTTTGGG+TGG + chr2.4:4641192-4641211 None:intergenic 45.0%
! AGAGCACTTGGTGATTACCT+TGG - chr2.4:4640692-4640711 MS.gene85235:CDS 45.0%
! CATGTTGTTGTTGGTACTCC+TGG - chr2.4:4640785-4640804 MS.gene85235:CDS 45.0%
! TTTTGTGAACACAAGGCGCA+AGG - chr2.4:4641410-4641429 MS.gene85235:intron 45.0%
!! CAGGAGTACCAACAACAACA+TGG + chr2.4:4640787-4640806 None:intergenic 45.0%
!! GAGAAGGTTATGAGAGCACT+TGG - chr2.4:4640680-4640699 MS.gene85235:CDS 45.0%
!! GTTTTGGTCCATGTCTCCAT+GGG + chr2.4:4641487-4641506 None:intergenic 45.0%
!! TTATGGTTTGGTTCAGTGCC+AGG - chr2.4:4640616-4640635 MS.gene85235:CDS 45.0%
!!! AGTGGGCGTTTTGGAAGAAA+AGG - chr2.4:4641666-4641685 MS.gene85235:intron 45.0%
!!! TGTTTTGGTCCATGTCTCCA+TGG + chr2.4:4641488-4641507 None:intergenic 45.0%
!!! TTATTTTTAGACAGGGGGCC+TGG - chr2.4:4640092-4640111 MS.gene85235:CDS 45.0%
!!! TTTGGTTTTGGCCCCAACAA+GGG - chr2.4:4640640-4640659 MS.gene85235:CDS 45.0%
AAGACAGCGACGTTCTGTTC+TGG - chr2.4:4640575-4640594 MS.gene85235:CDS 50.0%
AGCATGTCAAACACACGTCC+AGG + chr2.4:4640806-4640825 None:intergenic 50.0%
ATCATGCGTGAATTCCGCTC+TGG - chr2.4:4641519-4641538 MS.gene85235:intron 50.0%
CATCAATACCACGGGCCAAA+AGG + chr2.4:4641572-4641591 None:intergenic 50.0%
CTGCCACCCAAAATTCAGGT+TGG - chr2.4:4641186-4641205 MS.gene85235:intron 50.0%
GCGTGATGAATTGACCCTTG+AGG - chr2.4:4641290-4641309 MS.gene85235:intron 50.0%
GTTGCTGCCACCCAAAATTC+AGG - chr2.4:4641182-4641201 MS.gene85235:intron 50.0%
TCCATCGTATTGGGCGAAGT+GGG - chr2.4:4641649-4641668 MS.gene85235:intron 50.0%
TGCTGCGAAGACAGTCTCTT+CGG - chr2.4:4640822-4640841 MS.gene85235:CDS 50.0%
TTCACGTGGTTTCAAGGACC+AGG - chr2.4:4640892-4640911 MS.gene85235:CDS 50.0%
! CCCCTTTGCTGTATTGCAGA+TGG + chr2.4:4640497-4640516 None:intergenic 50.0%
! TTTGGTTCAGTGCCAGGCTT+TGG - chr2.4:4640622-4640641 MS.gene85235:CDS 50.0%
!! GAATTTTGGGTGGCAGCAAC+TGG + chr2.4:4641182-4641201 None:intergenic 50.0%
!! TCAGTGCCAGGCTTTGGTTT+TGG - chr2.4:4640628-4640647 MS.gene85235:CDS 50.0%
!!! CTTTGGTTTTGGCCCCAACA+AGG - chr2.4:4640639-4640658 MS.gene85235:CDS 50.0%
CATACTGTCTCAGCCACCCA+TGG - chr2.4:4641468-4641487 MS.gene85235:intron 55.0%
CATGGGTGGCTGAGACAGTA+TGG + chr2.4:4641470-4641489 None:intergenic 55.0%
CCACGACTACAACTCTTGCC+AGG + chr2.4:4640113-4640132 None:intergenic 55.0%
CCTGGCAAGAGTTGTAGTCG+TGG - chr2.4:4640110-4640129 MS.gene85235:CDS 55.0%
CTCCATCGTATTGGGCGAAG+TGG - chr2.4:4641648-4641667 MS.gene85235:intron 55.0%
CTTGTGATCTCCAGAGCCTC+TGG + chr2.4:4641234-4641253 None:intergenic 55.0%
GAACTCGAGAAGAGCCAGAG+CGG + chr2.4:4641536-4641555 None:intergenic 55.0%
GCTGGCACAGCAGATTGAGA+AGG - chr2.4:4640664-4640683 MS.gene85235:CDS 55.0%
GGGTCTCGATGTGATTCAGC+AGG - chr2.4:4640535-4640554 MS.gene85235:intron 55.0%
GTGATTCAGCAGGCTCAGTC+AGG - chr2.4:4640545-4640564 MS.gene85235:CDS 55.0%
TACAATGCCTCCAGAGGCTC+TGG - chr2.4:4641221-4641240 MS.gene85235:intron 55.0%
TGGCTCACTGACAAGATGCG+AGG - chr2.4:4641438-4641457 MS.gene85235:intron 55.0%
TGGTGGCACTAGTGTTCGTG+AGG - chr2.4:4640736-4640755 MS.gene85235:CDS 55.0%
TGTGAACACAAGGCGCAAGG+TGG - chr2.4:4641413-4641432 MS.gene85235:intron 55.0%
TTGGGGCCAAAACCAAAGCC+TGG + chr2.4:4640637-4640656 None:intergenic 55.0%
TTGGTCCATGTCTCCATGGG+TGG + chr2.4:4641484-4641503 None:intergenic 55.0%
! ATGGCAGGTTTAGCTCCGGA+AGG - chr2.4:4639650-4639669 MS.gene85235:CDS 55.0%
! ATTGGGCGAAGTGGGCGTTT+TGG - chr2.4:4641657-4641676 MS.gene85235:intron 55.0%
!! AGCCTCTGGAGGCATTGTAG+CGG + chr2.4:4641220-4641239 None:intergenic 55.0%
!! CAGAGGCTCTGGAGATCACA+AGG - chr2.4:4641232-4641251 MS.gene85235:intron 55.0%
ACACAAGGCGCAAGGTGGAC+TGG - chr2.4:4641418-4641437 MS.gene85235:intron 60.0%
ACCACGGGCCAAAAGGTCAG+TGG + chr2.4:4641565-4641584 None:intergenic 60.0%
CAGCAGGCTCAGTCAGGAAC+TGG - chr2.4:4640551-4640570 MS.gene85235:CDS 60.0%
CTCAGCCACCCATGGAGACA+TGG - chr2.4:4641476-4641495 MS.gene85235:intron 60.0%
CTCCGCTACAATGCCTCCAG+AGG - chr2.4:4641215-4641234 MS.gene85235:intron 60.0%
CTGTGCCAGCTCCCTTGTTG+GGG + chr2.4:4640654-4640673 None:intergenic 60.0%
GCCCACTTCGCCCAATACGA+TGG + chr2.4:4641653-4641672 None:intergenic 60.0%
GCTGTGCCAGCTCCCTTGTT+GGG + chr2.4:4640655-4640674 None:intergenic 60.0%
GTGATCTCCAGAGCCTCTGG+AGG + chr2.4:4641231-4641250 None:intergenic 60.0%
TGCTGTGCCAGCTCCCTTGT+TGG + chr2.4:4640656-4640675 None:intergenic 60.0%
TTTGGCCCCAACAAGGGAGC+TGG - chr2.4:4640646-4640665 MS.gene85235:CDS 60.0%
! ACCACTGACCTTTTGGCCCG+TGG - chr2.4:4641561-4641580 MS.gene85235:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4639650 4641800 4639650 ID=MS.gene85235
chr2.4 mRNA 4639650 4641800 4639650 ID=MS.gene85235.t1;Parent=MS.gene85235
chr2.4 exon 4641733 4641800 4641733 ID=MS.gene85235.t1.exon1;Parent=MS.gene85235.t1
chr2.4 CDS 4641733 4641800 4641733 ID=cds.MS.gene85235.t1;Parent=MS.gene85235.t1
chr2.4 exon 4641547 4641653 4641547 ID=MS.gene85235.t1.exon2;Parent=MS.gene85235.t1
chr2.4 CDS 4641547 4641653 4641547 ID=cds.MS.gene85235.t1;Parent=MS.gene85235.t1
chr2.4 exon 4640537 4640967 4640537 ID=MS.gene85235.t1.exon3;Parent=MS.gene85235.t1
chr2.4 CDS 4640537 4640967 4640537 ID=cds.MS.gene85235.t1;Parent=MS.gene85235.t1
chr2.4 exon 4639650 4640285 4639650 ID=MS.gene85235.t1.exon4;Parent=MS.gene85235.t1
chr2.4 CDS 4639650 4640285 4639650 ID=cds.MS.gene85235.t1;Parent=MS.gene85235.t1
Gene Sequence

>MS.gene85235

ATGGCAGGTTTAGCTCCGGAAGGTTCGCAATTTGATACCAAGCAGTTTGACACTAAGATGAATGAATTGTAAGTAAGGAAGTAATGCATGATTTTGGTCTGTATTATATTCTGTAAATCATGAAGTAATGCATAATTTTAATTACAGGCTTTCCACTGAAGGACAAGAATTCTTCACCTCATACGATGAAGTTCATGAAACTTTTGATGCAATGGGATTGATAGAAAATCTTCTGAGAGGCATATATGCTTATGGTATACAAATGACTTTTCCTTTTTTCTTTAAGCACTTATTAAGCATGTATGATAACAGTGTCAGTATGCAATTAGCATTAACCCAATTAGTGCCTATCACAATTTATTAATCCTTTTGGCAAAAGTTTAAAAATTAATTGGGTGCTGGACTAGCTATATTTTGAGAACTCTTTAAAAATCATGAACATTTATTTTTAGACAGGGGGCCTGGCAAGAGTTGTAGTCGTGGCCATTAATTAATTAACTCAAGGTTATATAATGGGTTTGACTTGACCCCTTGATAGTTGTCACTATATAGCCCCATGCTTTTCATTGATATATACAATCAATTTCGAGGGCAGCTACTTCTTTTCTTTAATCGCATGTTCGGAAAGTTATGTTTTGTAAATTTATCTGTTTCTTAATATTCAAATTTTAGACGATTAAAATTGTATTTTCTTAACCGTTCTTATTACGTTTTAACCTAAATACATCTATACACGTAGTAGTACTAGTATTTCATTTTAAAGAATATTGAAACTTTTTATGCACTTGACCTCACTAGCATACCATGTGTGATGTTCTGTTCTTGTGCTTAAGGTTTTGAGAAGCCATCTGCAATACAGCAAAGGGGAATTGTTCCTTTTATCAAGGGTCTCGATGTGATTCAGCAGGCTCAGTCAGGAACTGGAAAGACAGCGACGTTCTGTTCTGGAATTTTGCAGCAGCTTGATTATGGTTTGGTTCAGTGCCAGGCTTTGGTTTTGGCCCCAACAAGGGAGCTGGCACAGCAGATTGAGAAGGTTATGAGAGCACTTGGTGATTACCTTGGTGTTAAGGTTCATGCTTGTGTTGGTGGCACTAGTGTTCGTGAGGATCAGCGTATTCTCCAAACTGGTGTCCATGTTGTTGTTGGTACTCCTGGACGTGTGTTTGACATGCTGCGAAGACAGTCTCTTCGGTCAGATTGCATTAAAATGTTTGTTTTGGATGAAGCTGATGAAATGCTTTCACGTGGTTTCAAGGACCAGGTGTGTTAGATTTCTGATTGTTGGAACTGAATTTGGTTGGAGTTGGGTTTCTGATTGTTTGTTAGAACTGAATTTGGTTGAAATGTTTTTTTTTTGTTGCGCTCTCATGCACATGTTTCATGCATGTTATGTCTCACTACTTTTCCATATTCTATAACCCTTTACCTTCATTTGACCTACTTCTCACCAGATGAATTGATCTTGTTTTAAATAACTAACAGTTGATATTCTTATATGTTTGAAAAATGCAGATCTACGACATCTTCCAGTTGCTGCCACCCAAAATTCAGGTTGGAGTGTTCTCCGCTACAATGCCTCCAGAGGCTCTGGAGATCACAAGGAAGTTCATGAATAAGCCAGTTAGAATTCTGGTAAAGCGTGATGAATTGACCCTTGAGGGTATCAAGCAGTTCTACGTGAATGTTGACAAGGAAGAGTGGAAACTAGAGACATTGTGTGATCTTTATGAGACTCTGGCTATCACTCAGAGTGTCATTTTTGTGAACACAAGGCGCAAGGTGGACTGGCTCACTGACAAGATGCGAGGCAGAGACCATACTGTCTCAGCCACCCATGGAGACATGGACCAAAACACTCGTGATATCATCATGCGTGAATTCCGCTCTGGCTCTTCTCGAGTTCTCATTACCACTGACCTTTTGGCCCGTGGTATTGATGTTCAGCAAGTGTCTCTGGTCATAAATTATGATTTGCCTACCCAACCTGAAAACTATCTCCATCGTATTGGGCGAAGTGGGCGTTTTGGAAGAAAAGGTGTTGCTATCAACTTCGTCACATTGGAAGATGCAAGAATGCTGGCTGATATTCAGAAGTTCTATAATGTGATCGTAGAGGAATTGCCTTCAAATGTTGCTGATCTACTCTGA
Protein sequence

>MS.gene85235.t1

MAGLAPEGSQFDTKQFDTKMNELLSTEGQEFFTSYDEVHETFDAMGLIENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQTGVHVVVGTPGRVFDMLRRQSLRSDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRGRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTLEDARMLADIQKFYNVIVEELPSNVADLL