AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032228.t1 MTR_2g099620 99.758 413 1 0 1 413 1 413 0.0 850
MS.gene032228.t1 MTR_8g018400 94.189 413 24 0 1 413 1 413 0.0 808
MS.gene032228.t1 MTR_2g099620 98.734 395 5 0 19 413 3 397 0.0 803
MS.gene032228.t1 MTR_2g039960 92.978 413 29 0 1 413 1 413 0.0 800
MS.gene032228.t1 MTR_4g125970 91.525 413 35 0 1 413 18 430 0.0 781
MS.gene032228.t1 MTR_4g125970 91.525 413 35 0 1 413 1 413 0.0 781
MS.gene032228.t1 MTR_2g438110 64.611 373 132 0 41 413 34 406 0.0 517
MS.gene032228.t1 MTR_1g046570 61.168 394 116 5 56 413 6 398 4.28e-164 467
MS.gene032228.t1 MTR_8g018370 65.607 346 92 4 71 413 3 324 3.80e-152 433
MS.gene032228.t1 MTR_7g076800 40.984 366 212 4 42 406 142 504 9.22e-89 278
MS.gene032228.t1 MTR_7g076820 41.257 366 211 4 42 406 151 513 1.11e-88 278
MS.gene032228.t1 MTR_7g076830 40.984 366 212 4 42 406 144 506 1.34e-88 278
MS.gene032228.t1 MTR_3g107420 39.691 388 227 7 28 409 33 419 8.19e-79 250
MS.gene032228.t1 MTR_1g012420 38.660 388 231 7 28 409 33 419 3.20e-78 248
MS.gene032228.t1 MTR_4g010565 39.370 381 224 7 35 409 117 496 4.31e-76 245
MS.gene032228.t1 MTR_3g467550 35.467 375 220 5 40 396 116 486 1.96e-67 224
MS.gene032228.t1 MTR_1g061970 36.341 399 214 13 41 405 80 472 1.71e-65 217
MS.gene032228.t1 MTR_3g467550 35.326 368 216 5 47 396 92 455 1.77e-65 218
MS.gene032228.t1 MTR_8g018350 63.905 169 47 5 252 413 15 176 3.02e-65 206
MS.gene032228.t1 MTR_2g100230 35.068 365 223 3 40 394 95 455 6.09e-65 216
MS.gene032228.t1 MTR_4g049910 35.417 384 222 9 41 405 55 431 6.80e-65 214
MS.gene032228.t1 MTR_0915s0010 39.437 284 168 4 54 336 2 282 3.23e-62 202
MS.gene032228.t1 MTR_4g134170 34.301 379 217 7 47 397 293 667 4.54e-62 213
MS.gene032228.t1 MTR_7g109720 34.530 362 230 4 40 394 231 592 6.71e-62 213
MS.gene032228.t1 MTR_6g005320 34.748 377 216 7 47 397 255 627 1.73e-61 210
MS.gene032228.t1 MTR_3g449250 34.865 370 230 5 45 406 94 460 5.86e-61 207
MS.gene032228.t1 MTR_3g449250 32.452 416 258 6 11 406 48 460 1.01e-60 206
MS.gene032228.t1 MTR_8g018330 58.333 168 59 4 252 413 15 177 4.69e-60 193
MS.gene032228.t1 MTR_6g082080 33.065 372 245 3 40 408 9 379 8.38e-60 201
MS.gene032228.t1 MTR_3g083690 34.190 389 233 7 41 407 145 532 1.28e-59 204
MS.gene032228.t1 MTR_7g056830 33.065 372 245 3 40 408 9 379 1.89e-59 200
MS.gene032228.t1 MTR_4g105100 33.773 379 241 4 24 396 189 563 2.02e-59 204
MS.gene032228.t1 MTR_5g090120 34.043 376 235 6 45 411 85 456 4.62e-59 202
MS.gene032228.t1 MTR_2g007000 34.286 385 237 6 40 412 503 883 1.19e-56 201
MS.gene032228.t1 MTR_4g079320 32.976 373 240 5 42 407 20 389 2.21e-56 198
MS.gene032228.t1 MTR_2g032630 33.978 362 232 4 41 396 496 856 7.34e-56 199
MS.gene032228.t1 MTR_2g066050 34.167 360 232 2 41 395 53 412 1.29e-55 191
MS.gene032228.t1 MTR_4g114120 33.418 392 232 7 42 407 141 529 4.15e-55 193
MS.gene032228.t1 MTR_1g054630 33.165 395 230 9 40 407 153 540 8.28e-55 191
MS.gene032228.t1 MTR_3g467550 34.524 336 198 5 40 357 116 447 9.28e-55 188
MS.gene032228.t1 MTR_1g026830 30.513 390 252 8 40 413 146 532 8.96e-54 188
MS.gene032228.t1 MTR_1g095810 30.513 390 252 8 40 413 144 530 4.78e-53 186
MS.gene032228.t1 MTR_6g005550 31.316 380 250 5 41 412 165 541 7.78e-52 185
MS.gene032228.t1 MTR_3g049990 32.812 384 239 7 27 396 135 513 6.05e-51 183
MS.gene032228.t1 MTR_5g027840 33.803 355 221 5 42 384 105 457 1.87e-50 178
MS.gene032228.t1 MTR_6g033070 31.978 369 229 7 41 394 140 501 6.52e-48 171
MS.gene032228.t1 MTR_2g041140 31.593 383 246 7 27 396 124 503 2.62e-46 170
MS.gene032228.t1 MTR_2g055850 31.522 368 229 12 40 391 90 450 4.88e-43 158
MS.gene032228.t1 MTR_7g100820 28.718 390 253 6 34 412 130 505 1.83e-42 158
MS.gene032228.t1 MTR_4g112340 30.726 358 226 10 50 391 106 457 2.36e-42 157
MS.gene032228.t1 MTR_6g056080 31.054 351 215 6 45 380 115 453 2.82e-42 158
MS.gene032228.t1 MTR_4g105100 31.595 326 213 4 24 343 189 510 2.45e-40 150
MS.gene032228.t1 MTR_2g063080 26.173 405 234 7 40 382 108 509 1.34e-39 149
MS.gene032228.t1 MTR_8g027780 29.167 384 245 10 42 406 107 482 1.15e-38 147
MS.gene032228.t1 MTR_3g071620 29.396 364 229 7 47 383 22 384 1.83e-38 146
MS.gene032228.t1 MTR_1g102410 28.926 363 223 5 49 380 52 410 1.45e-37 144
MS.gene032228.t1 MTR_8g027775 28.646 384 247 9 42 406 103 478 2.08e-37 143
MS.gene032228.t1 MTR_7g057250 26.077 441 236 10 41 399 163 595 5.16e-37 143
MS.gene032228.t1 MTR_8g027770 28.385 384 248 8 42 406 202 577 2.74e-36 140
MS.gene032228.t1 MTR_8g097000 27.344 384 257 7 42 406 289 669 9.90e-35 137
MS.gene032228.t1 MTR_3g107420 41.463 205 116 4 35 235 40 244 2.38e-34 130
MS.gene032228.t1 MTR_1g083130 26.406 409 246 8 41 397 26 431 7.07e-34 133
MS.gene032228.t1 MTR_6g039840 28.312 385 238 11 41 396 110 485 2.85e-33 132
MS.gene032228.t1 MTR_4g068990 28.947 342 224 10 68 395 102 438 3.81e-33 132
MS.gene032228.t1 MTR_5g092560 27.062 388 261 9 46 413 81 466 2.70e-32 129
MS.gene032228.t1 MTR_4g112320 30.605 281 182 8 117 391 2 275 8.29e-32 125
MS.gene032228.t1 MTR_8g027780 29.518 332 211 10 42 352 107 436 1.97e-28 116
MS.gene032228.t1 MTR_5g069330 26.478 389 266 7 36 413 2 381 4.08e-28 115
MS.gene032228.t1 MTR_5g069330 26.494 385 263 7 40 413 2 377 6.48e-28 114
MS.gene032228.t1 MTR_3g118120 26.933 401 239 13 24 383 120 507 1.85e-25 108
MS.gene032228.t1 MTR_4g131750 25.700 393 237 13 49 399 90 469 2.07e-25 108
MS.gene032228.t1 MTR_6g039840 29.085 306 188 9 110 395 12 308 4.60e-25 107
MS.gene032228.t1 MTR_0427s0010 32.035 231 107 7 198 405 3 206 9.17e-25 101
MS.gene032228.t1 MTR_4g077370 36.126 191 119 1 36 223 245 435 7.18e-24 103
MS.gene032228.t1 MTR_4g077370 35.079 191 96 4 41 223 2 172 3.61e-18 86.7
MS.gene032228.t1 MTR_5g077690 22.872 376 225 6 79 389 71 446 5.61e-22 98.2
MS.gene032228.t1 MTR_1g022305 25.588 340 181 11 63 395 26 300 1.13e-20 94.0
MS.gene032228.t1 MTR_4g068990 29.464 224 145 6 181 395 8 227 4.42e-20 92.8
MS.gene032228.t1 MTR_5g092560 28.112 249 162 6 181 413 8 255 7.87e-19 89.0
MS.gene032228.t1 MTR_8g032230 36.429 140 82 2 40 175 9 145 9.57e-19 83.6
MS.gene032228.t1 MTR_4g459350 31.285 179 114 4 41 210 41 219 2.22e-18 85.1
MS.gene032228.t1 MTR_4g059460 31.285 179 114 4 41 210 44 222 1.22e-17 85.5
MS.gene032228.t1 MTR_4g059460 35.106 94 61 0 310 403 392 485 1.09e-13 73.2
MS.gene032228.t1 MTR_8g042690 23.238 383 264 13 41 404 18 389 2.07e-17 84.3
MS.gene032228.t1 MTR_2g043360 30.726 179 115 4 41 210 41 219 3.87e-17 84.0
MS.gene032228.t1 MTR_2g043360 35.052 97 62 1 310 406 389 484 3.07e-14 75.1
MS.gene032228.t1 MTR_2g043360 35.052 97 62 1 310 406 271 366 2.79e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032228.t1 AT1G54270 92.995 414 26 2 1 413 1 412 0.0 792
MS.gene032228.t1 AT3G13920 92.512 414 28 2 1 413 1 412 0.0 788
MS.gene032228.t1 AT1G54270 91.787 414 26 3 1 413 1 407 0.0 775
MS.gene032228.t1 AT3G13920 91.304 414 28 3 1 413 1 407 0.0 770
MS.gene032228.t1 AT1G72730 89.372 414 43 1 1 413 1 414 0.0 769
MS.gene032228.t1 AT3G13920 94.330 388 21 1 27 413 15 402 0.0 755
MS.gene032228.t1 AT3G13920 92.366 393 27 2 1 392 1 391 0.0 746
MS.gene032228.t1 AT3G13920 92.366 393 27 2 1 392 5 395 0.0 745
MS.gene032228.t1 AT3G19760 62.725 389 138 1 25 413 27 408 0.0 511
MS.gene032228.t1 AT1G51380 58.108 370 155 0 40 409 22 391 1.28e-162 462
MS.gene032228.t1 AT1G51380 58.103 253 106 0 40 292 22 274 7.50e-108 319
MS.gene032228.t1 AT3G61240 40.650 369 215 4 42 409 126 491 2.81e-90 281
MS.gene032228.t1 AT3G61240 40.650 369 215 4 42 409 126 491 2.81e-90 281
MS.gene032228.t1 AT2G45810 40.872 367 213 4 42 407 156 519 1.21e-88 278
MS.gene032228.t1 AT4G00660 39.837 369 218 4 42 409 133 498 1.29e-88 278
MS.gene032228.t1 AT4G00660 39.837 369 218 4 42 409 133 498 1.29e-88 278
MS.gene032228.t1 AT1G51380 55.660 212 94 0 40 251 22 233 2.05e-82 255
MS.gene032228.t1 AT1G51380 55.660 212 94 0 40 251 22 233 3.86e-82 254
MS.gene032228.t1 AT5G11170 38.660 388 231 7 28 409 33 419 2.14e-77 246
MS.gene032228.t1 AT5G11200 38.660 388 231 7 28 409 33 419 2.14e-77 246
MS.gene032228.t1 AT5G11170 39.169 337 199 6 78 409 1 336 1.04e-69 224
MS.gene032228.t1 AT5G11200 34.965 429 231 8 28 409 33 460 6.25e-69 225
MS.gene032228.t1 AT3G53110 34.988 403 235 10 26 407 81 477 2.06e-67 222
MS.gene032228.t1 AT1G55150 34.626 361 230 2 40 394 99 459 9.57e-66 218
MS.gene032228.t1 AT1G55150 34.626 361 230 2 40 394 124 484 1.10e-65 218
MS.gene032228.t1 AT5G26742 35.238 420 243 10 9 413 80 485 2.27e-64 219
MS.gene032228.t1 AT5G26742 35.238 420 243 10 9 413 80 485 2.30e-64 219
MS.gene032228.t1 AT5G63120 34.085 399 247 4 24 413 153 544 3.71e-64 216
MS.gene032228.t1 AT5G26742 36.458 384 212 10 45 413 26 392 1.46e-63 216
MS.gene032228.t1 AT2G33730 34.987 383 221 6 41 397 314 694 1.76e-63 217
MS.gene032228.t1 AT3G22310 35.484 372 227 6 45 407 121 488 1.54e-62 212
MS.gene032228.t1 AT3G22310 35.484 372 227 6 45 407 121 488 2.04e-62 212
MS.gene032228.t1 AT3G22330 35.215 372 228 6 45 407 109 476 3.70e-62 211
MS.gene032228.t1 AT2G47330 34.359 390 238 7 37 412 222 607 2.87e-60 209
MS.gene032228.t1 AT1G77030 32.527 372 243 4 42 407 30 399 1.63e-59 207
MS.gene032228.t1 AT3G06480 35.156 384 230 6 41 412 436 812 4.97e-59 208
MS.gene032228.t1 AT1G20920 32.850 414 263 7 9 412 163 571 1.31e-58 205
MS.gene032228.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene032228.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene032228.t1 AT3G58510 33.247 385 237 7 41 406 152 535 4.61e-58 200
MS.gene032228.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene032228.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene032228.t1 AT2G42520 35.000 400 230 10 33 404 149 546 6.10e-58 200
MS.gene032228.t1 AT3G58570 33.753 397 240 8 33 406 136 532 3.71e-57 198
MS.gene032228.t1 AT5G14610 32.808 381 243 6 41 412 162 538 5.23e-57 198
MS.gene032228.t1 AT5G14610 32.808 381 243 6 41 412 162 538 5.77e-57 198
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT1G20920 34.301 379 233 6 46 412 535 909 6.47e-57 202
MS.gene032228.t1 AT5G14610 32.808 381 243 6 41 412 170 546 6.71e-57 198
MS.gene032228.t1 AT5G60990 32.071 396 245 5 36 413 5 394 1.68e-56 192
MS.gene032228.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.15e-56 195
MS.gene032228.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene032228.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene032228.t1 AT3G01540 34.555 382 235 10 41 412 159 535 4.62e-56 195
MS.gene032228.t1 AT1G16280 33.158 380 238 5 30 396 43 419 1.46e-55 191
MS.gene032228.t1 AT4G16630 33.867 375 239 4 31 396 155 529 2.07e-54 193
MS.gene032228.t1 AT5G14610 32.153 367 236 6 55 412 45 407 1.95e-53 186
MS.gene032228.t1 AT5G14610 32.153 367 236 6 55 412 45 407 1.95e-53 186
MS.gene032228.t1 AT5G11200 31.991 447 238 11 28 409 33 478 8.39e-53 183
MS.gene032228.t1 AT3G02065 32.603 365 233 5 41 394 111 473 2.88e-52 182
MS.gene032228.t1 AT3G02065 32.603 365 233 5 41 394 111 473 2.88e-52 182
MS.gene032228.t1 AT5G14610 32.249 369 237 6 53 412 242 606 3.46e-52 186
MS.gene032228.t1 AT4G33370 30.848 389 250 8 40 412 97 482 6.58e-52 182
MS.gene032228.t1 AT1G28180 34.694 343 208 6 79 410 12 349 1.45e-51 177
MS.gene032228.t1 AT5G51280 30.591 389 251 7 40 412 146 531 1.62e-51 182
MS.gene032228.t1 AT1G31970 33.075 387 241 6 40 413 116 497 2.38e-51 181
MS.gene032228.t1 AT3G18600 31.649 376 247 6 41 408 90 463 3.07e-49 176
MS.gene032228.t1 AT3G09620 32.447 376 209 7 46 409 402 744 4.80e-49 179
MS.gene032228.t1 AT3G09620 32.447 376 209 7 46 409 403 745 5.20e-49 179
MS.gene032228.t1 AT3G09720 29.921 381 260 4 34 412 138 513 1.56e-48 173
MS.gene032228.t1 AT3G09720 29.921 381 260 4 34 412 138 513 1.56e-48 173
MS.gene032228.t1 AT3G02065 32.628 331 210 5 75 394 8 336 7.56e-47 165
MS.gene032228.t1 AT1G12770 27.363 402 230 6 40 381 110 509 8.93e-47 169
MS.gene032228.t1 AT1G12770 27.363 402 230 6 40 381 110 509 8.93e-47 169
MS.gene032228.t1 AT5G65900 30.667 375 251 6 38 404 152 525 1.41e-45 167
MS.gene032228.t1 AT5G62190 32.181 376 227 9 55 411 111 477 2.49e-45 166
MS.gene032228.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene032228.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene032228.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.45e-44 161
MS.gene032228.t1 AT5G63120 33.435 329 203 4 24 343 153 474 1.88e-44 161
MS.gene032228.t1 AT3G06480 33.333 324 197 6 41 352 436 752 1.71e-41 156
MS.gene032228.t1 AT5G05450 30.168 358 225 7 50 383 26 382 5.86e-41 153
MS.gene032228.t1 AT1G71370 30.114 352 230 7 47 383 23 373 3.01e-40 151
MS.gene032228.t1 AT5G14610 31.024 332 216 6 41 363 170 497 3.93e-40 149
MS.gene032228.t1 AT5G63630 29.948 384 242 8 42 406 56 431 9.99e-39 146
MS.gene032228.t1 AT5G08620 30.151 398 235 9 42 412 82 463 1.73e-38 146
MS.gene032228.t1 AT5G08610 30.318 409 237 11 28 406 369 759 1.84e-38 148
MS.gene032228.t1 AT5G63630 29.948 384 242 8 42 406 250 625 1.45e-37 145
MS.gene032228.t1 AT2G07750 27.157 394 248 10 42 407 377 759 3.79e-35 138
MS.gene032228.t1 AT1G63250 26.866 402 248 10 35 406 321 706 1.17e-33 134
MS.gene032228.t1 AT4G34910 26.098 387 229 8 40 372 46 429 3.55e-32 129
MS.gene032228.t1 AT5G54910 27.273 396 263 12 17 396 49 435 1.23e-31 127
MS.gene032228.t1 AT5G19210 27.763 389 231 12 49 399 85 461 1.57e-31 125
MS.gene032228.t1 AT2G40700 25.225 444 251 12 41 403 31 474 3.61e-31 125
MS.gene032228.t1 AT4G09730 26.753 385 246 9 40 396 112 488 4.02e-31 125
MS.gene032228.t1 AT3G06980 28.500 400 239 14 21 383 360 749 5.48e-28 117
MS.gene032228.t1 AT1G71280 27.419 310 189 9 47 345 24 308 2.40e-26 108
MS.gene032228.t1 AT4G15850 25.648 386 211 13 79 394 74 453 4.77e-26 110
MS.gene032228.t1 AT1G71280 27.419 310 189 9 47 345 24 308 1.62e-25 108
MS.gene032228.t1 AT3G16840 32.821 195 107 4 41 211 191 385 1.90e-19 91.3
MS.gene032228.t1 AT3G16840 32.821 195 107 4 41 211 192 386 1.98e-19 91.3
MS.gene032228.t1 AT5G19210 26.032 315 183 11 123 399 2 304 1.98e-17 83.2
MS.gene032228.t1 AT5G19210 26.032 315 183 11 123 399 2 304 1.98e-17 83.2
MS.gene032228.t1 AT1G28180 37.273 110 55 2 79 177 12 118 1.02e-11 62.4

Find 89 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACACTCCAACCTGAATTT+TGG 0.146444 2.3:+4044213 None:intergenic
TCTCATTACCACTGACCTTT+TGG 0.272997 2.3:-4043852 MS.gene032228:CDS
ACATCGAGACCCTTGATAAA+AGG 0.302219 2.3:+4044885 None:intergenic
TGCTGTGCCAGCTCCCTTGT+TGG 0.308505 2.3:+4044756 None:intergenic
TCAGTGCCAGGCTTTGGTTT+TGG 0.312026 2.3:-4044781 MS.gene032228:CDS
GTTGCTGCCACCCAAAATTC+AGG 0.315611 2.3:-4044224 MS.gene032228:CDS
GCTGTGCCAGCTCCCTTGTT+GGG 0.317476 2.3:+4044757 None:intergenic
TTGCATTAAAATGTTTGTTT+TGG 0.318028 2.3:-4044559 MS.gene032228:CDS
CGCTTTACCAGAATTCTAAC+TGG 0.324777 2.3:+4044136 None:intergenic
AAGACAGCGACGTTCTGTTC+TGG 0.339543 2.3:-4044834 MS.gene032228:CDS
TGGTGATTACCTTGGTGTTA+AGG 0.345997 2.3:-4044709 MS.gene032228:CDS
TGATGATATCACGAGTGTTT+TGG 0.362515 2.3:+4043906 None:intergenic
GAATAAGCCAGTTAGAATTC+TGG 0.365648 2.3:-4044143 MS.gene032228:CDS
CTGAGAGGCATATATGCTTA+TGG 0.371983 2.3:-4045508 MS.gene032228:intron
GTATCAAATTGCGAACCTTC+CGG 0.376954 2.3:+4045725 None:intergenic
TTATGGTTTGGTTCAGTGCC+AGG 0.388767 2.3:-4044793 MS.gene032228:CDS
AAAACTATCTCCATCGTATT+GGG 0.393330 2.3:-4043766 MS.gene032228:CDS
CAACAACATGGACACCAGTT+TGG 0.395614 2.3:+4044637 None:intergenic
CTTGTGATCTCCAGAGCCTC+TGG 0.397309 2.3:+4044175 None:intergenic
GCAGCAGCTTGATTATGGTT+TGG 0.397488 2.3:-4044805 MS.gene032228:CDS
TTTGGTTCAGTGCCAGGCTT+TGG 0.398715 2.3:-4044787 MS.gene032228:CDS
GAAAACTATCTCCATCGTAT+TGG 0.406801 2.3:-4043767 MS.gene032228:CDS
ATTGGGCGAAGTGGGCGTTT+TGG 0.409374 2.3:-4043749 MS.gene032228:CDS
CATGTTGTTGTTGGTACTCC+TGG 0.424537 2.3:-4044624 MS.gene032228:CDS
AATGCTTTCACGTGGTTTCA+AGG 0.432515 2.3:-4044523 MS.gene032228:CDS
CCCCTTTGCTGTATTGCAGA+TGG 0.433851 2.3:+4044915 None:intergenic
GCCATCTGCAATACAGCAAA+GGG 0.435434 2.3:-4044916 MS.gene032228:CDS
TTCATGAACTTCATCGTATG+AGG 0.438056 2.3:+4045564 None:intergenic
CGTGATGAATTGACCCTTGA+GGG 0.439342 2.3:-4044115 MS.gene032228:CDS
CATGGGTGGCTGAGACAGTA+TGG 0.446776 2.3:+4043939 None:intergenic
TGATGTTCAGCAAGTGTCTC+TGG 0.448249 2.3:-4043819 MS.gene032228:CDS
CAGCAGGCTCAGTCAGGAAC+TGG 0.451120 2.3:-4044858 MS.gene032228:CDS
TACAATGCCTCCAGAGGCTC+TGG 0.459621 2.3:-4044185 MS.gene032228:CDS
CATCAATACCACGGGCCAAA+AGG 0.460041 2.3:+4043837 None:intergenic
TTTGGCCCCAACAAGGGAGC+TGG 0.463851 2.3:-4044763 MS.gene032228:CDS
AAGGTTCATGCTTGTGTTGG+TGG 0.466235 2.3:-4044690 MS.gene032228:CDS
AGTAGATCAGCAACATTTGA+AGG 0.481306 2.3:+4043632 None:intergenic
TGCTGCGAAGACAGTCTCTT+CGG 0.488354 2.3:-4044587 MS.gene032228:CDS
GTGATTCAGCAGGCTCAGTC+AGG 0.498167 2.3:-4044864 MS.gene032228:CDS
CATCTTAGTGTCAAACTGCT+TGG 0.499196 2.3:+4045703 None:intergenic
CTCCATCGTATTGGGCGAAG+TGG 0.499221 2.3:-4043758 MS.gene032228:CDS
ACTGGTGTCCATGTTGTTGT+TGG 0.499852 2.3:-4044633 MS.gene032228:CDS
GATCAGCGTATTCTCCAAAC+TGG 0.501639 2.3:-4044651 MS.gene032228:CDS
TTCACGTGGTTTCAAGGACC+AGG 0.504125 2.3:-4044517 MS.gene032228:intron
ACACAAGGCGCAAGGTGGAC+TGG 0.505122 2.3:-4043988 MS.gene032228:CDS
GTTAAGGTTCATGCTTGTGT+TGG 0.507288 2.3:-4044693 MS.gene032228:CDS
GTGTGATCTTTATGAGACTC+TGG 0.521304 2.3:-4044038 MS.gene032228:CDS
ATCATGCGTGAATTCCGCTC+TGG 0.527239 2.3:-4043887 MS.gene032228:CDS
GAAGAATTCTTGTCCTTCAG+TGG 0.531706 2.3:+4045588 None:intergenic
GAGAAGGTTATGAGAGCACT+TGG 0.533228 2.3:-4044729 MS.gene032228:CDS
AATTACAGGCTTTCCACTGA+AGG 0.540134 2.3:-4045601 MS.gene032228:intron
CTGTGCCAGCTCCCTTGTTG+GGG 0.543113 2.3:+4044758 None:intergenic
TGCTATCAACTTCGTCACAT+TGG 0.543489 2.3:-4043714 MS.gene032228:CDS
AGCATGTCAAACACACGTCC+AGG 0.544974 2.3:+4044606 None:intergenic
GGGTCTCGATGTGATTCAGC+AGG 0.549184 2.3:-4044874 MS.gene032228:CDS
CTCTGCTACAATGCCTCCAG+AGG 0.551425 2.3:-4044191 MS.gene032228:CDS
TTGGGGCCAAAACCAAAGCC+TGG 0.554251 2.3:+4044775 None:intergenic
CAGAGGCTCTGGAGATCACA+AGG 0.564855 2.3:-4044174 MS.gene032228:CDS
CTCAGCCACCCATGGAGACA+TGG 0.565513 2.3:-4043930 MS.gene032228:CDS
TCCATCGTATTGGGCGAAGT+GGG 0.570459 2.3:-4043757 MS.gene032228:CDS
CTGCCACCCAAAATTCAGGT+TGG 0.574116 2.3:-4044220 MS.gene032228:CDS
TTGGTCCATGTCTCCATGGG+TGG 0.574389 2.3:+4043925 None:intergenic
AGTCATGGCAGGTTTAGCTC+CGG 0.585504 2.3:-4045744 None:intergenic
AGCCATCTGCAATACAGCAA+AGG 0.592072 2.3:-4044917 MS.gene032228:CDS
ATTGGAAGATGCAAGAATGC+TGG 0.599752 2.3:-4043696 MS.gene032228:CDS
GCCCACTTCGCCCAATACGA+TGG 0.601432 2.3:+4043756 None:intergenic
AGAACTGCTTGATACCCTCA+AGG 0.605423 2.3:+4044101 None:intergenic
CAGGAGTACCAACAACAACA+TGG 0.608453 2.3:+4044625 None:intergenic
GTGATCTCCAGAGCCTCTGG+AGG 0.613832 2.3:+4044178 None:intergenic
TGAATGTTGACAAGGAAGAG+TGG 0.615932 2.3:-4044075 MS.gene032228:CDS
TTGCTGAACATCAATACCAC+GGG 0.620577 2.3:+4043829 None:intergenic
GCTGGCACAGCAGATTGAGA+AGG 0.628451 2.3:-4044745 MS.gene032228:CDS
CATACTGTCTCAGCCACCCA+TGG 0.631002 2.3:-4043938 MS.gene032228:CDS
ATGGCAGGTTTAGCTCCGGA+AGG 0.641220 2.3:-4045740 MS.gene032228:CDS
AGAGCACTTGGTGATTACCT+TGG 0.644049 2.3:-4044717 MS.gene032228:CDS
GTTCTATAATGTGATCGTAG+AGG 0.657829 2.3:-4043660 MS.gene032228:CDS
CTTGCTGAACATCAATACCA+CGG 0.663030 2.3:+4043828 None:intergenic
GCGTGATGAATTGACCCTTG+AGG 0.663843 2.3:-4044116 MS.gene032228:CDS
ACCACGGGCCAAAAGGTCAG+TGG 0.665992 2.3:+4043844 None:intergenic
TTGATAGAAAATCTTCTGAG+AGG 0.671045 2.3:-4045523 MS.gene032228:CDS
GAACTGCTTGATACCCTCAA+GGG 0.677590 2.3:+4044102 None:intergenic
TGGCTCACTGACAAGATGCG+AGG 0.683723 2.3:-4043968 MS.gene032228:CDS
TGGTGGCACTAGTGTTCGTG+AGG 0.685653 2.3:-4044673 MS.gene032228:CDS
GTTCTACGTGAATGTTGACA+AGG 0.687466 2.3:-4044083 MS.gene032228:CDS
GAACTCGAGAAGAGCCAGAG+CGG 0.689835 2.3:+4043873 None:intergenic
TGTGAACACAAGGCGCAAGG+TGG 0.709342 2.3:-4043993 MS.gene032228:CDS
AAGCATGAACCTTAACACCA+AGG 0.715621 2.3:+4044700 None:intergenic
GCTGATGAAATGCTTTCACG+TGG 0.720414 2.3:-4044531 MS.gene032228:CDS
CCATCTGCAATACAGCAAAG+GGG 0.725959 2.3:-4044915 MS.gene032228:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAGTTTAAAAATTAATT+GGG - chr2.3:4044001-4044020 MS.gene032228:CDS 10.0%
!! GCAAAAGTTTAAAAATTAAT+TGG - chr2.3:4044000-4044019 MS.gene032228:CDS 15.0%
!!! TAATGCATAATTTTAATTAC+AGG - chr2.3:4043753-4043772 MS.gene032228:CDS 15.0%
!! AAGGATTAAAAAATTGTGAT+AGG + chr2.3:4043977-4043996 None:intergenic 20.0%
!! ATTAACTCAAGGTTATATAA+TGG - chr2.3:4044103-4044122 MS.gene032228:CDS 20.0%
!! GCAATTAATTAATTAACTCA+AGG - chr2.3:4044092-4044111 MS.gene032228:CDS 20.0%
!! GTATATATAAACAATTTCGA+GGG - chr2.3:4044179-4044198 MS.gene032228:CDS 20.0%
!! TTAACTCAAGGTTATATAAT+GGG - chr2.3:4044104-4044123 MS.gene032228:CDS 20.0%
!! TTATCATACATGCTTAATAA+GGG + chr2.3:4043917-4043936 None:intergenic 20.0%
!!! ATTTTTAAACTTTTGCCAAA+AGG + chr2.3:4043996-4044015 None:intergenic 20.0%
!!! TCACAATTTTTTAATCCTTT+TGG - chr2.3:4043978-4043997 MS.gene032228:CDS 20.0%
!!! TGAAAACTCTTTAAAAATCA+TGG - chr2.3:4044043-4044062 MS.gene032228:CDS 20.0%
!!! TTGCATTAAAATGTTTGTTT+TGG - chr2.3:4044809-4044828 MS.gene032228:CDS 20.0%
! AATAAGGGCTTAAAGAAAAA+AGG + chr2.3:4043902-4043921 None:intergenic 25.0%
! AGATGAATGAATTGTAAGTA+AGG - chr2.3:4043683-4043702 MS.gene032228:CDS 25.0%
! AGGTTAAAACGTAATAAGAA+CGG + chr2.3:4044307-4044326 None:intergenic 25.0%
! GGTATATATAAACAATTTCG+AGG - chr2.3:4044178-4044197 MS.gene032228:CDS 25.0%
! GTTATCATACATGCTTAATA+AGG + chr2.3:4043918-4043937 None:intergenic 25.0%
! TAAAACAAGATCAATTCATC+TGG + chr2.3:4045065-4045084 None:intergenic 25.0%
! TACAGTGTATAGATGTATTT+AGG + chr2.3:4044327-4044346 None:intergenic 25.0%
! TATATAGTGACAACTATCAA+GGG + chr2.3:4044141-4044160 None:intergenic 25.0%
! TTTAAAAATTAATTGGGTGC+TGG - chr2.3:4044007-4044026 MS.gene032228:CDS 25.0%
!! TTGTTTGTTAGAACTGAATT+TGG - chr2.3:4044927-4044946 MS.gene032228:CDS 25.0%
!!! ATGGACATTTATTTTTAGAC+AGG - chr2.3:4044062-4044081 MS.gene032228:CDS 25.0%
!!! TGGACATTTATTTTTAGACA+GGG - chr2.3:4044063-4044082 MS.gene032228:CDS 25.0%
AAAACTATCTCCATCGTATT+GGG - chr2.3:4045602-4045621 MS.gene032228:intron 30.0%
AAATTGTGATAGGCACTAAT+TGG + chr2.3:4043967-4043986 None:intergenic 30.0%
AATTGTGATAGGCACTAATT+GGG + chr2.3:4043966-4043985 None:intergenic 30.0%
AGGTAAAGGGTTATAGAATA+TGG + chr2.3:4045023-4045042 None:intergenic 30.0%
ATATAGTGACAACTATCAAG+GGG + chr2.3:4044140-4044159 None:intergenic 30.0%
ATATATACCAATGACAAGCA+TGG + chr2.3:4044167-4044186 None:intergenic 30.0%
CTATATAGTGACAACTATCA+AGG + chr2.3:4044142-4044161 None:intergenic 30.0%
TATATACCAATGACAAGCAT+GGG + chr2.3:4044166-4044185 None:intergenic 30.0%
TGTGTTAGATTTCTGATTGT+TGG - chr2.3:4044874-4044893 MS.gene032228:CDS 30.0%
TTGATAGAAAATCTTCTGAG+AGG - chr2.3:4043845-4043864 MS.gene032228:CDS 30.0%
! CATGAAACTTTTGATGCAAT+GGG - chr2.3:4043821-4043840 MS.gene032228:CDS 30.0%
! CTTTTCTTTAATCGCATGTT+CGG - chr2.3:4044211-4044230 MS.gene032228:CDS 30.0%
! GGAATTGTTCCTTTTATCAA+GGG - chr2.3:4044474-4044493 MS.gene032228:intron 30.0%
! TCATGAAACTTTTGATGCAA+TGG - chr2.3:4043820-4043839 MS.gene032228:CDS 30.0%
!!! GACATTTATTTTTAGACAGG+GGG - chr2.3:4044065-4044084 MS.gene032228:CDS 30.0%
!!! GGACATTTATTTTTAGACAG+GGG - chr2.3:4044064-4044083 MS.gene032228:CDS 30.0%
AAAAATGACACTCTGAGTGA+TGG + chr2.3:4045356-4045375 None:intergenic 35.0%
AACTGAATTTGGTTGGAGTT+GGG - chr2.3:4044897-4044916 MS.gene032228:CDS 35.0%
AATCAGAAATCTAACACACC+TGG + chr2.3:4044872-4044891 None:intergenic 35.0%
AGTAGATCAGCAACATTTGA+AGG + chr2.3:4045739-4045758 None:intergenic 35.0%
AGTAGGTCAAATGAAGGTAA+AGG + chr2.3:4045037-4045056 None:intergenic 35.0%
ATATACCAATGACAAGCATG+GGG + chr2.3:4044165-4044184 None:intergenic 35.0%
ATGTTCTGTTCTTGTGCTTA+AGG - chr2.3:4044420-4044439 MS.gene032228:intron 35.0%
CTGATTGTTGGAACTGAATT+TGG - chr2.3:4044886-4044905 MS.gene032228:CDS 35.0%
GAAAACTATCTCCATCGTAT+TGG - chr2.3:4045601-4045620 MS.gene032228:intron 35.0%
GAATAAGCCAGTTAGAATTC+TGG - chr2.3:4045225-4045244 MS.gene032228:intron 35.0%
GTAGGTCAAATGAAGGTAAA+GGG + chr2.3:4045036-4045055 None:intergenic 35.0%
GTTCTATAATGTGATCGTAG+AGG - chr2.3:4045708-4045727 MS.gene032228:CDS 35.0%
TTCATGAACTTCATCGTATG+AGG + chr2.3:4043807-4043826 None:intergenic 35.0%
TTGTTGGAACTGAATTTGGT+TGG - chr2.3:4044890-4044909 MS.gene032228:CDS 35.0%
! AACACTCCAACCTGAATTTT+GGG + chr2.3:4045157-4045176 None:intergenic 35.0%
! ATACGATGGAGATAGTTTTC+AGG + chr2.3:4045601-4045620 None:intergenic 35.0%
! ATTTTGCAGCAGCTTGATTA+TGG - chr2.3:4044558-4044577 MS.gene032228:CDS 35.0%
! GGGAATTGTTCCTTTTATCA+AGG - chr2.3:4044473-4044492 MS.gene032228:intron 35.0%
! TGATGATATCACGAGTGTTT+TGG + chr2.3:4045465-4045484 None:intergenic 35.0%
!!! ATGGAGATAGTTTTCAGGTT+GGG + chr2.3:4045596-4045615 None:intergenic 35.0%
!!! GTGTCATTTTTGTGAACACA+AGG - chr2.3:4045365-4045384 MS.gene032228:intron 35.0%
AAGCATGAACCTTAACACCA+AGG + chr2.3:4044671-4044690 None:intergenic 40.0%
AATGCTTTCACGTGGTTTCA+AGG - chr2.3:4044845-4044864 MS.gene032228:CDS 40.0%
AATTACAGGCTTTCCACTGA+AGG - chr2.3:4043767-4043786 MS.gene032228:CDS 40.0%
ACATCGAGACCCTTGATAAA+AGG + chr2.3:4044486-4044505 None:intergenic 40.0%
ATTGGAAGATGCAAGAATGC+TGG - chr2.3:4045672-4045691 MS.gene032228:intron 40.0%
CAAGAACAGAACATCACACA+TGG + chr2.3:4044414-4044433 None:intergenic 40.0%
CAATTCATCTGGTGAGAAGT+AGG + chr2.3:4045054-4045073 None:intergenic 40.0%
CATCTTAGTGTCAAACTGCT+TGG + chr2.3:4043668-4043687 None:intergenic 40.0%
CGCTTTACCAGAATTCTAAC+TGG + chr2.3:4045235-4045254 None:intergenic 40.0%
CTTGCTGAACATCAATACCA+CGG + chr2.3:4045543-4045562 None:intergenic 40.0%
GAACTGAATTTGGTTGGAGT+TGG - chr2.3:4044896-4044915 MS.gene032228:CDS 40.0%
GAAGAATTCTTGTCCTTCAG+TGG + chr2.3:4043783-4043802 None:intergenic 40.0%
GTATCAAATTGCGAACCTTC+CGG + chr2.3:4043646-4043665 None:intergenic 40.0%
GTGAGAAGTAGGTCAAATGA+AGG + chr2.3:4045043-4045062 None:intergenic 40.0%
GTGTGATCTTTATGAGACTC+TGG - chr2.3:4045330-4045349 MS.gene032228:intron 40.0%
GTTAAGGTTCATGCTTGTGT+TGG - chr2.3:4044675-4044694 MS.gene032228:CDS 40.0%
GTTCTACGTGAATGTTGACA+AGG - chr2.3:4045285-4045304 MS.gene032228:intron 40.0%
TGAATGTTGACAAGGAAGAG+TGG - chr2.3:4045293-4045312 MS.gene032228:intron 40.0%
TGCTATCAACTTCGTCACAT+TGG - chr2.3:4045654-4045673 MS.gene032228:intron 40.0%
TTGCTGAACATCAATACCAC+GGG + chr2.3:4045542-4045561 None:intergenic 40.0%
! CTGAGAGGCATATATGCTTA+TGG - chr2.3:4043860-4043879 MS.gene032228:CDS 40.0%
! GAACACTCCAACCTGAATTT+TGG + chr2.3:4045158-4045177 None:intergenic 40.0%
! TCTCATTACCACTGACCTTT+TGG - chr2.3:4045516-4045535 MS.gene032228:CDS 40.0%
!! TGGTGATTACCTTGGTGTTA+AGG - chr2.3:4044659-4044678 MS.gene032228:CDS 40.0%
!!! AGATAGTTTTCAGGTTGGGT+AGG + chr2.3:4045592-4045611 None:intergenic 40.0%
!!! GATGGAGATAGTTTTCAGGT+TGG + chr2.3:4045597-4045616 None:intergenic 40.0%
!!! TTATTTTTAGACAGGGGGCT+TGG - chr2.3:4044070-4044089 MS.gene032228:CDS 40.0%
AAGGTTCATGCTTGTGTTGG+TGG - chr2.3:4044678-4044697 MS.gene032228:CDS 45.0%
ACTGGTGTCCATGTTGTTGT+TGG - chr2.3:4044735-4044754 MS.gene032228:CDS 45.0%
AGAACTGCTTGATACCCTCA+AGG + chr2.3:4045270-4045289 None:intergenic 45.0%
AGCCATCTGCAATACAGCAA+AGG - chr2.3:4044451-4044470 MS.gene032228:intron 45.0%
CAACAACATGGACACCAGTT+TGG + chr2.3:4044734-4044753 None:intergenic 45.0%
CCATCTGCAATACAGCAAAG+GGG - chr2.3:4044453-4044472 MS.gene032228:intron 45.0%
CGTGATGAATTGACCCTTGA+GGG - chr2.3:4045253-4045272 MS.gene032228:intron 45.0%
GAACTGCTTGATACCCTCAA+GGG + chr2.3:4045269-4045288 None:intergenic 45.0%
GATCAGCGTATTCTCCAAAC+TGG - chr2.3:4044717-4044736 MS.gene032228:CDS 45.0%
GCAGCAGCTTGATTATGGTT+TGG - chr2.3:4044563-4044582 MS.gene032228:CDS 45.0%
GCCATCTGCAATACAGCAAA+GGG - chr2.3:4044452-4044471 MS.gene032228:intron 45.0%
GCTGATGAAATGCTTTCACG+TGG - chr2.3:4044837-4044856 MS.gene032228:CDS 45.0%
TATAGCCCCATGCTTGTCAT+TGG - chr2.3:4044157-4044176 MS.gene032228:CDS 45.0%
TCACACATGGTATGCTAGTG+AGG + chr2.3:4044401-4044420 None:intergenic 45.0%
TGATGTTCAGCAAGTGTCTC+TGG - chr2.3:4045549-4045568 MS.gene032228:CDS 45.0%
! ACTCCAACCTGAATTTTGGG+TGG + chr2.3:4045154-4045173 None:intergenic 45.0%
! AGAGCACTTGGTGATTACCT+TGG - chr2.3:4044651-4044670 MS.gene032228:CDS 45.0%
! CATGTTGTTGTTGGTACTCC+TGG - chr2.3:4044744-4044763 MS.gene032228:CDS 45.0%
! TTTTGTGAACACAAGGCGCA+AGG - chr2.3:4045372-4045391 MS.gene032228:intron 45.0%
!! CAGGAGTACCAACAACAACA+TGG + chr2.3:4044746-4044765 None:intergenic 45.0%
!! GAGAAGGTTATGAGAGCACT+TGG - chr2.3:4044639-4044658 MS.gene032228:CDS 45.0%
!! GTTTTGGTCCATGTCTCCAT+GGG + chr2.3:4045449-4045468 None:intergenic 45.0%
!! TTATGGTTTGGTTCAGTGCC+AGG - chr2.3:4044575-4044594 MS.gene032228:CDS 45.0%
!!! AGTGGGCGTTTTGGAAGAAA+AGG - chr2.3:4045628-4045647 MS.gene032228:intron 45.0%
!!! TGTTTTGGTCCATGTCTCCA+TGG + chr2.3:4045450-4045469 None:intergenic 45.0%
!!! TTTGGTTTTGGCCCCAACAA+GGG - chr2.3:4044599-4044618 MS.gene032228:CDS 45.0%
AAGACAGCGACGTTCTGTTC+TGG - chr2.3:4044534-4044553 MS.gene032228:CDS 50.0%
AGCATGTCAAACACACGTCC+AGG + chr2.3:4044765-4044784 None:intergenic 50.0%
ATCATGCGTGAATTCCGCTC+TGG - chr2.3:4045481-4045500 MS.gene032228:intron 50.0%
CATCAATACCACGGGCCAAA+AGG + chr2.3:4045534-4045553 None:intergenic 50.0%
CTGCCACCCAAAATTCAGGT+TGG - chr2.3:4045148-4045167 MS.gene032228:intron 50.0%
GCGTGATGAATTGACCCTTG+AGG - chr2.3:4045252-4045271 MS.gene032228:intron 50.0%
GTTGCTGCCACCCAAAATTC+AGG - chr2.3:4045144-4045163 MS.gene032228:intron 50.0%
TCCATCGTATTGGGCGAAGT+GGG - chr2.3:4045611-4045630 MS.gene032228:intron 50.0%
TGCTGCGAAGACAGTCTCTT+CGG - chr2.3:4044781-4044800 MS.gene032228:CDS 50.0%
TTCACGTGGTTTCAAGGACC+AGG - chr2.3:4044851-4044870 MS.gene032228:CDS 50.0%
! CCCCTTTGCTGTATTGCAGA+TGG + chr2.3:4044456-4044475 None:intergenic 50.0%
! TTTGGTTCAGTGCCAGGCTT+TGG - chr2.3:4044581-4044600 MS.gene032228:CDS 50.0%
!! GAATTTTGGGTGGCAGCAAC+TGG + chr2.3:4045144-4045163 None:intergenic 50.0%
!! TCAGTGCCAGGCTTTGGTTT+TGG - chr2.3:4044587-4044606 MS.gene032228:CDS 50.0%
!!! CTTTGGTTTTGGCCCCAACA+AGG - chr2.3:4044598-4044617 MS.gene032228:CDS 50.0%
CATACTGTCTCAGCCACCCA+TGG - chr2.3:4045430-4045449 MS.gene032228:intron 55.0%
CATGGGTGGCTGAGACAGTA+TGG + chr2.3:4045432-4045451 None:intergenic 55.0%
CTCCATCGTATTGGGCGAAG+TGG - chr2.3:4045610-4045629 MS.gene032228:intron 55.0%
CTCTGCTACAATGCCTCCAG+AGG - chr2.3:4045177-4045196 MS.gene032228:intron 55.0%
CTTGTGATCTCCAGAGCCTC+TGG + chr2.3:4045196-4045215 None:intergenic 55.0%
GAACTCGAGAAGAGCCAGAG+CGG + chr2.3:4045498-4045517 None:intergenic 55.0%
GCTGGCACAGCAGATTGAGA+AGG - chr2.3:4044623-4044642 MS.gene032228:CDS 55.0%
GGGTCTCGATGTGATTCAGC+AGG - chr2.3:4044494-4044513 MS.gene032228:intron 55.0%
GTGATTCAGCAGGCTCAGTC+AGG - chr2.3:4044504-4044523 MS.gene032228:intron 55.0%
TACAATGCCTCCAGAGGCTC+TGG - chr2.3:4045183-4045202 MS.gene032228:intron 55.0%
TGGCTCACTGACAAGATGCG+AGG - chr2.3:4045400-4045419 MS.gene032228:intron 55.0%
TGGTGGCACTAGTGTTCGTG+AGG - chr2.3:4044695-4044714 MS.gene032228:CDS 55.0%
TGTGAACACAAGGCGCAAGG+TGG - chr2.3:4045375-4045394 MS.gene032228:intron 55.0%
TTGGGGCCAAAACCAAAGCC+TGG + chr2.3:4044596-4044615 None:intergenic 55.0%
TTGGTCCATGTCTCCATGGG+TGG + chr2.3:4045446-4045465 None:intergenic 55.0%
! ATGGCAGGTTTAGCTCCGGA+AGG - chr2.3:4043628-4043647 MS.gene032228:CDS 55.0%
! ATTGGGCGAAGTGGGCGTTT+TGG - chr2.3:4045619-4045638 MS.gene032228:intron 55.0%
!! CAGAGGCTCTGGAGATCACA+AGG - chr2.3:4045194-4045213 MS.gene032228:intron 55.0%
ACACAAGGCGCAAGGTGGAC+TGG - chr2.3:4045380-4045399 MS.gene032228:intron 60.0%
ACCACGGGCCAAAAGGTCAG+TGG + chr2.3:4045527-4045546 None:intergenic 60.0%
CAGCAGGCTCAGTCAGGAAC+TGG - chr2.3:4044510-4044529 MS.gene032228:intron 60.0%
CTCAGCCACCCATGGAGACA+TGG - chr2.3:4045438-4045457 MS.gene032228:intron 60.0%
CTGTGCCAGCTCCCTTGTTG+GGG + chr2.3:4044613-4044632 None:intergenic 60.0%
GCCCACTTCGCCCAATACGA+TGG + chr2.3:4045615-4045634 None:intergenic 60.0%
GCTGTGCCAGCTCCCTTGTT+GGG + chr2.3:4044614-4044633 None:intergenic 60.0%
GTGATCTCCAGAGCCTCTGG+AGG + chr2.3:4045193-4045212 None:intergenic 60.0%
TGCTGTGCCAGCTCCCTTGT+TGG + chr2.3:4044615-4044634 None:intergenic 60.0%
TTTGGCCCCAACAAGGGAGC+TGG - chr2.3:4044605-4044624 MS.gene032228:CDS 60.0%
! ACCACTGACCTTTTGGCCCG+TGG - chr2.3:4045523-4045542 MS.gene032228:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4043628 4045762 4043628 ID=MS.gene032228
chr2.3 mRNA 4043628 4045762 4043628 ID=MS.gene032228.t1;Parent=MS.gene032228
chr2.3 exon 4045695 4045762 4045695 ID=MS.gene032228.t1.exon1;Parent=MS.gene032228.t1
chr2.3 CDS 4045695 4045762 4045695 ID=cds.MS.gene032228.t1;Parent=MS.gene032228.t1
chr2.3 exon 4045509 4045615 4045509 ID=MS.gene032228.t1.exon2;Parent=MS.gene032228.t1
chr2.3 CDS 4045509 4045615 4045509 ID=cds.MS.gene032228.t1;Parent=MS.gene032228.t1
chr2.3 exon 4044518 4044948 4044518 ID=MS.gene032228.t1.exon3;Parent=MS.gene032228.t1
chr2.3 CDS 4044518 4044948 4044518 ID=cds.MS.gene032228.t1;Parent=MS.gene032228.t1
chr2.3 exon 4043628 4044263 4043628 ID=MS.gene032228.t1.exon4;Parent=MS.gene032228.t1
chr2.3 CDS 4043628 4044263 4043628 ID=cds.MS.gene032228.t1;Parent=MS.gene032228.t1
Gene Sequence

>MS.gene032228

ATGGCAGGTTTAGCTCCGGAAGGTTCGCAATTTGATACCAAGCAGTTTGACACTAAGATGAATGAATTGTAAGTAAGGAAGTAATGCATGATTTTTGTCTGTATTATATTCTGTAAATCATGAAGTAATGCATAATTTTAATTACAGGCTTTCCACTGAAGGACAAGAATTCTTCACCTCATACGATGAAGTTCATGAAACTTTTGATGCAATGGGATTGATAGAAAATCTTCTGAGAGGCATATATGCTTATGGTATACAAATGACTTTTCCTTTTTTCTTTAAGCCCTTATTAAGCATGTATGATAACAGTGTCAGTATGCAATTAGCATTAACCCAATTAGTGCCTATCACAATTTTTTAATCCTTTTGGCAAAAGTTTAAAAATTAATTGGGTGCTGGACTAGCTATATTTTGAAAACTCTTTAAAAATCATGGACATTTATTTTTAGACAGGGGGCTTGGCAATTAATTAATTAACTCAAGGTTATATAATGGGTTTGACTTGACCCCTTGATAGTTGTCACTATATAGCCCCATGCTTGTCATTGGTATATATAAACAATTTCGAGGGCAGCTACTTCTTTTCTTTAATCGCATGTTCGGAAAGTTATGTTTTGTAAATTTCTGTTTCTTAATATTCAAATTTTAGACGATTAAAATTGTATTTTCTTAACCGTTCTTATTACGTTTTAACCTAAATACATCTATACACTGTAGTAGTACTAGTATTTCATTTTAAAGAATATTGAAACTTTTTATGCACTTGACCTCACTAGCATACCATGTGTGATGTTCTGTTCTTGTGCTTAAGGTTTTGAGAAGCCATCTGCAATACAGCAAAGGGGAATTGTTCCTTTTATCAAGGGTCTCGATGTGATTCAGCAGGCTCAGTCAGGAACTGGAAAGACAGCGACGTTCTGTTCTGGAATTTTGCAGCAGCTTGATTATGGTTTGGTTCAGTGCCAGGCTTTGGTTTTGGCCCCAACAAGGGAGCTGGCACAGCAGATTGAGAAGGTTATGAGAGCACTTGGTGATTACCTTGGTGTTAAGGTTCATGCTTGTGTTGGTGGCACTAGTGTTCGTGAGGATCAGCGTATTCTCCAAACTGGTGTCCATGTTGTTGTTGGTACTCCTGGACGTGTGTTTGACATGCTGCGAAGACAGTCTCTTCGGTCAGATTGCATTAAAATGTTTGTTTTGGATGAAGCTGATGAAATGCTTTCACGTGGTTTCAAGGACCAGGTGTGTTAGATTTCTGATTGTTGGAACTGAATTTGGTTGGAGTTGGGTTTCTGATTGTTTGTTAGAACTGAATTTGGTTGAAATGTTTTTTTTTTTTTGTTGCGCTCTCATGCACATGTTTAATGCATGTTATGTCTCACTACTTTTCCATATTCTATAACCCTTTACCTTCATTTGACCTACTTCTCACCAGATGAATTGATCTTGTTTTAAATAACTAACAGTTGATATTCTTATATGTTTGAAAAATGCAGATCTACGACATCTTCCAGTTGCTGCCACCCAAAATTCAGGTTGGAGTGTTCTCTGCTACAATGCCTCCAGAGGCTCTGGAGATCACAAGGAAGTTCATGAATAAGCCAGTTAGAATTCTGGTAAAGCGTGATGAATTGACCCTTGAGGGTATCAAGCAGTTCTACGTGAATGTTGACAAGGAAGAGTGGAAACTAGAGACATTGTGTGATCTTTATGAGACTCTGGCCATCACTCAGAGTGTCATTTTTGTGAACACAAGGCGCAAGGTGGACTGGCTCACTGACAAGATGCGAGGCAGAGACCATACTGTCTCAGCCACCCATGGAGACATGGACCAAAACACTCGTGATATCATCATGCGTGAATTCCGCTCTGGCTCTTCTCGAGTTCTCATTACCACTGACCTTTTGGCCCGTGGTATTGATGTTCAGCAAGTGTCTCTGGTCATAAATTATGATTTGCCTACCCAACCTGAAAACTATCTCCATCGTATTGGGCGAAGTGGGCGTTTTGGAAGAAAAGGTGTTGCTATCAACTTCGTCACATTGGAAGATGCAAGAATGCTGGCTGATATTCAGAAGTTCTATAATGTGATCGTAGAGGAATTGCCTTCAAATGTTGCTGATCTACTCTGA
Protein sequence

>MS.gene032228.t1

MAGLAPEGSQFDTKQFDTKMNELLSTEGQEFFTSYDEVHETFDAMGLIENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQTGVHVVVGTPGRVFDMLRRQSLRSDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRGRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTLEDARMLADIQKFYNVIVEELPSNVADLL