AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042577

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042577.t1 MTR_6g033070 97.212 538 15 0 1 538 1 538 0.0 1074
MS.gene042577.t1 MTR_1g054630 34.783 414 254 5 134 534 148 558 1.78e-77 256
MS.gene042577.t1 MTR_2g100230 34.398 407 252 7 116 516 73 470 2.46e-75 247
MS.gene042577.t1 MTR_3g083690 34.058 414 255 6 132 530 137 547 1.09e-74 248
MS.gene042577.t1 MTR_4g114120 35.142 424 253 6 134 538 134 554 2.21e-72 243
MS.gene042577.t1 MTR_3g467550 34.949 392 240 6 115 494 89 477 3.41e-70 235
MS.gene042577.t1 MTR_6g005550 32.952 437 278 8 106 538 130 555 1.32e-69 237
MS.gene042577.t1 MTR_4g134170 32.304 421 252 8 121 516 267 679 7.25e-69 235
MS.gene042577.t1 MTR_6g005320 31.579 418 257 7 121 516 229 639 4.69e-68 232
MS.gene042577.t1 MTR_3g467550 35.475 358 219 5 146 494 92 446 1.87e-67 227
MS.gene042577.t1 MTR_4g105100 31.818 418 271 8 118 531 179 586 4.83e-66 226
MS.gene042577.t1 MTR_2g032630 32.861 423 269 7 103 516 453 869 2.86e-64 227
MS.gene042577.t1 MTR_1g095810 31.555 431 278 10 120 537 124 550 6.64e-63 216
MS.gene042577.t1 MTR_1g026830 30.841 428 279 9 119 533 125 548 2.37e-61 212
MS.gene042577.t1 MTR_3g467550 33.846 390 238 7 92 464 61 447 2.41e-61 209
MS.gene042577.t1 MTR_7g109720 30.310 419 275 8 109 518 199 609 6.62e-58 206
MS.gene042577.t1 MTR_5g027840 33.679 386 248 7 137 517 101 483 1.61e-57 200
MS.gene042577.t1 MTR_2g007000 30.673 401 269 6 121 516 484 880 6.09e-55 200
MS.gene042577.t1 MTR_7g100820 29.655 435 277 10 95 520 92 506 3.89e-53 190
MS.gene042577.t1 MTR_7g056830 32.584 356 226 5 140 491 10 355 1.14e-51 182
MS.gene042577.t1 MTR_3g049990 30.623 369 246 6 134 498 146 508 1.38e-51 188
MS.gene042577.t1 MTR_4g105100 32.184 348 227 7 118 461 179 521 1.89e-51 184
MS.gene042577.t1 MTR_6g082080 31.694 366 236 5 140 501 10 365 3.38e-51 181
MS.gene042577.t1 MTR_4g125970 32.418 364 234 6 140 501 41 394 1.24e-49 176
MS.gene042577.t1 MTR_4g125970 32.418 364 234 6 140 501 58 411 2.02e-49 176
MS.gene042577.t1 MTR_2g039960 32.143 364 235 6 140 501 41 394 2.20e-49 176
MS.gene042577.t1 MTR_2g041140 30.541 370 246 7 134 498 135 498 3.72e-49 181
MS.gene042577.t1 MTR_2g099620 32.249 369 228 7 140 501 25 378 6.34e-49 174
MS.gene042577.t1 MTR_2g099620 32.249 369 228 7 140 501 41 394 8.63e-49 174
MS.gene042577.t1 MTR_8g018400 31.868 364 236 6 140 501 41 394 1.09e-48 174
MS.gene042577.t1 MTR_7g076820 31.063 367 235 6 133 495 143 495 1.53e-48 176
MS.gene042577.t1 MTR_7g076830 30.518 367 237 6 133 495 136 488 2.54e-47 172
MS.gene042577.t1 MTR_2g438110 28.495 372 238 6 140 501 34 387 2.67e-47 170
MS.gene042577.t1 MTR_7g076800 30.518 367 237 6 133 495 134 486 2.86e-47 172
MS.gene042577.t1 MTR_5g090120 30.245 367 246 8 135 497 76 436 2.96e-47 174
MS.gene042577.t1 MTR_3g449250 30.978 368 244 8 132 496 82 442 2.08e-46 171
MS.gene042577.t1 MTR_3g449250 30.978 368 244 8 132 496 82 442 3.82e-46 170
MS.gene042577.t1 MTR_2g066050 29.974 387 251 7 140 521 53 424 9.54e-46 168
MS.gene042577.t1 MTR_4g079320 28.117 377 263 4 141 515 20 390 1.16e-45 171
MS.gene042577.t1 MTR_3g107420 30.357 392 241 10 110 491 24 393 3.30e-41 154
MS.gene042577.t1 MTR_4g010565 30.123 405 251 11 131 521 115 501 8.09e-41 154
MS.gene042577.t1 MTR_1g012420 30.914 372 230 10 131 491 38 393 2.94e-40 151
MS.gene042577.t1 MTR_2g055850 31.016 374 227 12 132 490 85 442 3.37e-40 153
MS.gene042577.t1 MTR_4g059460 27.556 450 250 11 140 517 44 489 7.56e-39 150
MS.gene042577.t1 MTR_4g112340 32.277 347 209 15 158 490 115 449 5.25e-38 147
MS.gene042577.t1 MTR_2g043360 27.294 425 232 10 140 490 41 462 7.48e-38 147
MS.gene042577.t1 MTR_6g039840 27.225 382 260 9 140 506 110 488 3.51e-37 146
MS.gene042577.t1 MTR_8g027775 30.387 362 239 10 141 490 103 463 5.34e-37 144
MS.gene042577.t1 MTR_3g071620 28.495 372 230 10 146 490 22 384 6.62e-37 144
MS.gene042577.t1 MTR_7g057250 25.986 431 247 9 140 501 163 590 4.14e-36 144
MS.gene042577.t1 MTR_8g027770 30.997 371 225 13 141 490 202 562 5.37e-36 142
MS.gene042577.t1 MTR_6g056080 27.988 343 232 6 164 497 127 463 1.71e-35 141
MS.gene042577.t1 MTR_8g027780 30.663 362 238 10 141 490 107 467 2.54e-35 140
MS.gene042577.t1 MTR_1g102410 25.464 377 250 6 146 497 50 420 4.21e-35 140
MS.gene042577.t1 MTR_0915s0010 30.272 294 187 6 154 443 3 282 1.08e-32 127
MS.gene042577.t1 MTR_3g118120 25.132 378 254 9 140 490 132 507 7.86e-32 129
MS.gene042577.t1 MTR_5g092560 26.567 335 233 9 163 490 99 427 3.21e-30 125
MS.gene042577.t1 MTR_1g083130 26.341 410 247 13 140 501 26 428 3.33e-30 125
MS.gene042577.t1 MTR_8g097000 27.119 413 268 13 117 504 259 663 1.06e-29 124
MS.gene042577.t1 MTR_1g061970 27.778 378 230 12 138 484 78 443 7.45e-29 120
MS.gene042577.t1 MTR_4g068990 26.269 335 233 9 163 490 99 426 3.79e-28 119
MS.gene042577.t1 MTR_8g027780 30.514 331 217 10 141 459 107 436 4.27e-27 114
MS.gene042577.t1 MTR_4g459350 32.407 216 123 5 140 333 41 255 3.04e-25 105
MS.gene042577.t1 MTR_6g039840 26.384 307 210 8 213 506 9 312 4.98e-25 108
MS.gene042577.t1 MTR_4g049910 26.823 384 236 10 138 497 53 415 1.12e-24 107
MS.gene042577.t1 MTR_4g112320 29.091 275 166 12 228 490 10 267 2.11e-23 102
MS.gene042577.t1 MTR_4g077370 34.975 203 121 5 139 337 249 444 3.60e-23 102
MS.gene042577.t1 MTR_4g077370 33.953 215 106 6 139 344 1 188 4.34e-21 96.3
MS.gene042577.t1 MTR_1g046570 26.087 391 235 12 155 506 6 381 3.70e-23 102
MS.gene042577.t1 MTR_4g131750 26.087 368 227 12 145 479 87 442 1.42e-22 101
MS.gene042577.t1 MTR_8g032230 36.129 155 92 3 140 293 10 158 1.96e-22 94.7
MS.gene042577.t1 MTR_5g069330 24.581 358 252 8 140 491 3 348 1.46e-20 94.7
MS.gene042577.t1 MTR_5g069330 24.581 358 252 8 140 491 7 352 1.74e-20 94.4
MS.gene042577.t1 MTR_5g077690 23.608 449 267 13 145 523 34 476 2.07e-20 94.7
MS.gene042577.t1 MTR_8g018370 26.113 337 212 9 169 501 2 305 8.04e-20 90.9
MS.gene042577.t1 MTR_2g063080 27.572 243 149 6 131 346 98 340 7.68e-19 90.1
MS.gene042577.t1 MTR_3g107420 28.980 245 144 8 110 344 24 248 8.18e-19 87.8
MS.gene042577.t1 MTR_2g043360 22.324 327 182 6 235 490 19 344 2.68e-18 88.2
MS.gene042577.t1 MTR_8g042690 23.034 356 219 12 163 490 41 369 1.07e-16 83.2
MS.gene042577.t1 MTR_5g092560 25.822 213 149 6 284 490 7 216 1.29e-14 77.0
MS.gene042577.t1 MTR_4g068990 25.822 213 148 7 284 490 7 215 4.26e-14 75.5
MS.gene042577.t1 MTR_2g006440 25.479 365 215 13 151 489 398 731 1.27e-13 74.3
MS.gene042577.t1 MTR_2g042440 26.087 345 204 14 158 479 39 355 1.65e-12 70.5
MS.gene042577.t1 MTR_2g042440 26.087 345 204 14 158 479 39 355 1.87e-12 70.5
MS.gene042577.t1 MTR_8g062720 28.436 211 124 7 139 331 90 291 5.99e-12 68.6
MS.gene042577.t1 MTR_3g063040 22.874 341 219 10 158 481 13 326 1.57e-11 67.8
MS.gene042577.t1 MTR_1g083840 24.737 380 246 11 146 508 186 542 3.35e-11 66.2
MS.gene042577.t1 MTR_8g080160 23.224 366 228 11 149 493 266 599 9.11e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042577.t1 AT3G02065 66.129 496 145 4 27 518 14 490 0.0 682
MS.gene042577.t1 AT3G02065 66.129 496 145 4 27 518 14 490 0.0 682
MS.gene042577.t1 AT3G02065 71.388 353 100 1 167 518 1 353 0.0 534
MS.gene042577.t1 AT1G55150 32.924 407 258 7 117 516 76 474 5.63e-75 246
MS.gene042577.t1 AT1G55150 32.924 407 258 7 117 516 101 499 2.21e-74 245
MS.gene042577.t1 AT5G14610 33.179 431 274 8 111 537 132 552 3.26e-72 243
MS.gene042577.t1 AT5G14610 33.179 431 274 8 111 537 132 552 3.73e-72 242
MS.gene042577.t1 AT5G14610 33.179 431 274 8 111 537 140 560 7.35e-72 242
MS.gene042577.t1 AT2G42520 33.813 417 247 7 134 530 154 561 1.01e-71 241
MS.gene042577.t1 AT2G42520 33.813 417 247 7 134 530 154 561 1.01e-71 241
MS.gene042577.t1 AT2G42520 33.813 417 247 7 134 530 154 561 1.01e-71 241
MS.gene042577.t1 AT5G63120 31.830 399 265 6 121 516 146 540 1.21e-71 240
MS.gene042577.t1 AT3G58570 33.573 417 248 7 134 530 141 548 1.58e-71 241
MS.gene042577.t1 AT3G01540 33.097 423 268 8 116 534 134 545 2.55e-70 237
MS.gene042577.t1 AT3G01540 33.097 423 268 8 116 534 134 545 2.55e-70 237
MS.gene042577.t1 AT3G01540 33.097 423 268 8 116 534 134 545 2.55e-70 237
MS.gene042577.t1 AT3G01540 33.097 423 268 8 116 534 134 545 2.95e-70 237
MS.gene042577.t1 AT3G58510 32.683 410 250 6 132 524 144 544 3.84e-70 236
MS.gene042577.t1 AT3G58510 32.683 410 250 6 132 524 144 544 3.84e-70 236
MS.gene042577.t1 AT3G58510 32.683 410 250 6 132 524 144 544 3.84e-70 236
MS.gene042577.t1 AT2G33730 31.765 425 257 7 121 520 294 710 5.19e-66 228
MS.gene042577.t1 AT3G06480 32.426 404 257 7 120 516 415 809 1.42e-64 228
MS.gene042577.t1 AT5G14610 32.817 387 247 7 154 537 45 421 1.39e-63 216
MS.gene042577.t1 AT5G14610 32.817 387 247 7 154 537 45 421 1.39e-63 216
MS.gene042577.t1 AT4G33370 32.564 433 275 10 118 537 75 503 1.18e-62 214
MS.gene042577.t1 AT5G14610 32.426 404 260 7 137 537 227 620 1.41e-62 218
MS.gene042577.t1 AT5G51280 32.512 406 260 8 118 513 124 525 2.30e-62 215
MS.gene042577.t1 AT1G31970 33.981 412 260 8 115 517 89 497 9.65e-62 212
MS.gene042577.t1 AT5G14610 32.884 371 234 8 111 477 140 499 2.28e-56 197
MS.gene042577.t1 AT1G28180 32.682 358 223 6 167 516 1 348 3.13e-56 193
MS.gene042577.t1 AT2G47330 30.198 404 266 7 123 518 211 606 2.81e-55 199
MS.gene042577.t1 AT5G63120 31.176 340 227 6 121 457 146 481 2.18e-54 191
MS.gene042577.t1 AT3G09720 30.414 411 257 9 121 518 118 512 3.35e-54 192
MS.gene042577.t1 AT3G09720 30.414 411 257 9 121 518 118 512 3.35e-54 192
MS.gene042577.t1 AT1G20920 29.557 406 277 6 116 516 167 568 8.04e-54 195
MS.gene042577.t1 AT4G16630 30.997 371 242 7 134 498 162 524 1.09e-53 194
MS.gene042577.t1 AT5G63120 31.231 333 222 6 121 450 146 474 2.42e-53 188
MS.gene042577.t1 AT5G63120 31.231 333 222 6 121 450 146 474 2.42e-53 188
MS.gene042577.t1 AT5G63120 31.231 333 222 6 121 450 146 474 2.42e-53 188
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT1G20920 29.657 408 274 7 116 516 505 906 4.11e-53 195
MS.gene042577.t1 AT3G06480 32.092 349 221 7 120 461 415 754 3.98e-51 187
MS.gene042577.t1 AT1G72730 32.418 364 234 5 140 501 42 395 8.08e-51 179
MS.gene042577.t1 AT3G13920 32.877 365 231 7 140 501 30 383 1.85e-50 178
MS.gene042577.t1 AT3G13920 32.877 365 231 7 140 501 40 393 2.03e-50 178
MS.gene042577.t1 AT3G13920 33.149 362 228 7 140 498 40 390 2.27e-50 178
MS.gene042577.t1 AT3G13920 33.149 362 228 7 140 498 44 394 2.31e-50 179
MS.gene042577.t1 AT4G00660 29.517 393 259 6 133 521 125 503 2.33e-50 181
MS.gene042577.t1 AT4G00660 29.517 393 259 6 133 521 125 503 2.33e-50 181
MS.gene042577.t1 AT1G51380 30.532 357 234 7 137 490 20 365 1.21e-49 176
MS.gene042577.t1 AT1G54270 33.609 363 226 8 140 498 40 391 2.25e-48 173
MS.gene042577.t1 AT3G19760 27.297 370 251 5 137 501 33 389 2.66e-48 172
MS.gene042577.t1 AT5G26742 31.351 370 241 8 139 501 102 465 1.85e-47 176
MS.gene042577.t1 AT5G26742 31.351 370 241 8 139 501 102 465 1.87e-47 176
MS.gene042577.t1 AT1G77030 30.056 356 241 4 141 494 30 379 1.89e-47 177
MS.gene042577.t1 AT3G13920 32.329 365 228 8 140 501 40 388 2.28e-47 170
MS.gene042577.t1 AT5G60990 31.025 361 235 6 140 491 10 365 3.52e-47 171
MS.gene042577.t1 AT3G61240 30.790 367 236 6 133 495 118 470 7.07e-47 171
MS.gene042577.t1 AT3G61240 30.790 367 236 6 133 495 118 470 7.07e-47 171
MS.gene042577.t1 AT2G45810 29.919 371 242 6 129 495 144 500 1.17e-45 168
MS.gene042577.t1 AT1G54270 33.058 363 223 9 140 498 40 386 2.02e-45 165
MS.gene042577.t1 AT3G09620 28.788 396 244 7 123 513 379 741 3.02e-45 171
MS.gene042577.t1 AT3G09620 28.788 396 244 7 123 513 380 742 3.02e-45 171
MS.gene042577.t1 AT3G22330 28.261 368 254 8 134 497 99 460 6.58e-45 167
MS.gene042577.t1 AT3G22310 29.462 353 240 7 139 488 116 462 7.90e-44 164
MS.gene042577.t1 AT3G22310 29.462 353 240 7 139 488 116 462 9.46e-44 164
MS.gene042577.t1 AT1G16280 29.973 367 240 7 135 494 54 410 9.64e-44 162
MS.gene042577.t1 AT5G26742 30.997 371 231 10 138 501 20 372 3.12e-43 163
MS.gene042577.t1 AT5G11170 29.639 415 267 11 114 521 28 424 3.19e-41 154
MS.gene042577.t1 AT5G11200 29.639 415 267 11 114 521 28 424 3.19e-41 154
MS.gene042577.t1 AT2G40700 29.062 437 233 13 140 501 31 465 2.44e-40 154
MS.gene042577.t1 AT1G71370 28.689 366 243 9 137 490 14 373 7.74e-40 152
MS.gene042577.t1 AT4G09730 24.935 385 273 6 140 510 113 495 1.68e-36 144
MS.gene042577.t1 AT5G08620 28.378 370 244 11 151 504 92 456 2.69e-35 139
MS.gene042577.t1 AT3G18600 28.351 388 259 10 131 513 83 456 2.93e-35 139
MS.gene042577.t1 AT5G11200 27.133 457 265 13 114 521 28 465 3.72e-35 138
MS.gene042577.t1 AT5G62190 27.019 359 243 6 150 497 107 457 4.39e-35 140
MS.gene042577.t1 AT5G05450 27.273 363 235 10 150 490 27 382 6.59e-35 139
MS.gene042577.t1 AT5G65900 28.385 384 253 10 140 515 155 524 1.92e-33 135
MS.gene042577.t1 AT5G63630 27.869 366 251 10 151 504 66 430 2.08e-33 134
MS.gene042577.t1 AT5G63630 27.869 366 251 10 151 504 260 624 4.82e-33 134
MS.gene042577.t1 AT3G16840 26.389 432 243 13 140 501 192 618 1.97e-32 132
MS.gene042577.t1 AT3G16840 26.389 432 243 13 140 501 191 617 2.32e-32 132
MS.gene042577.t1 AT5G08610 28.933 356 232 11 151 490 394 744 8.18e-32 130
MS.gene042577.t1 AT1G12770 25.854 410 249 10 138 495 109 515 1.77e-31 128
MS.gene042577.t1 AT1G12770 25.854 410 249 10 138 495 109 515 1.77e-31 128
MS.gene042577.t1 AT5G54910 26.087 391 269 11 136 519 68 445 2.71e-31 129
MS.gene042577.t1 AT5G11170 29.769 346 225 10 183 521 7 341 5.19e-31 124
MS.gene042577.t1 AT3G06980 24.868 378 254 9 140 490 375 749 5.01e-28 119
MS.gene042577.t1 AT4G34910 26.873 387 232 11 140 479 47 429 6.20e-28 118
MS.gene042577.t1 AT1G51380 27.984 243 164 4 137 378 20 252 9.48e-28 112
MS.gene042577.t1 AT3G53110 25.616 406 250 12 121 493 68 454 1.16e-26 113
MS.gene042577.t1 AT1G63250 26.810 373 247 11 151 504 340 705 2.58e-26 114
MS.gene042577.t1 AT1G71280 27.302 315 191 9 146 452 24 308 6.45e-26 108
MS.gene042577.t1 AT1G71280 27.358 318 193 9 146 455 24 311 9.91e-26 110
MS.gene042577.t1 AT1G51380 29.108 213 143 4 137 348 20 225 2.78e-25 106
MS.gene042577.t1 AT1G51380 29.108 213 143 4 137 348 20 225 3.99e-25 106
MS.gene042577.t1 AT2G07750 26.542 373 248 11 151 504 387 752 9.25e-25 109
MS.gene042577.t1 AT5G19210 25.138 362 233 9 146 479 83 434 2.10e-23 103
MS.gene042577.t1 AT4G15850 23.326 433 254 11 149 513 41 463 3.07e-21 97.4
MS.gene042577.t1 AT1G28180 34.483 116 75 1 167 281 1 116 6.66e-19 83.2
MS.gene042577.t1 AT5G11200 28.632 234 147 7 114 342 28 246 1.20e-17 86.3
MS.gene042577.t1 AT5G11200 29.703 202 137 4 321 521 286 483 9.79e-15 77.0
MS.gene042577.t1 AT1G10930 26.462 359 219 15 151 489 443 776 2.95e-16 82.8
MS.gene042577.t1 AT3G05740 25.587 383 239 15 146 508 106 462 4.24e-14 75.5
MS.gene042577.t1 AT3G05740 25.587 383 239 15 146 508 191 547 7.94e-14 74.7
MS.gene042577.t1 AT1G27880 23.714 350 233 12 158 494 266 594 5.14e-13 72.4
MS.gene042577.t1 AT1G27880 23.782 349 232 12 158 493 266 593 1.08e-12 71.2
MS.gene042577.t1 AT4G35740 23.034 356 219 13 166 495 41 367 4.08e-11 66.2

Find 123 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCACAACGAGAAATTAT+TGG 0.221104 6.3:+21237504 None:intergenic
GGTTGTGGCTACTGGAGTTT+TGG 0.244181 6.3:-21236773 MS.gene042577:CDS
AGAAATAATGCAGTCATTTA+TGG 0.244375 6.3:-21236812 MS.gene042577:CDS
CGACATGCCTAATTCGATTA+AGG 0.248513 6.3:-21236707 MS.gene042577:CDS
CAGGTGGCTTAAAATGTTTA+TGG 0.265325 6.3:+21236950 None:intergenic
CCGTGAAGCAGCTAGCTATT+TGG 0.265897 6.3:-21237020 MS.gene042577:CDS
TTCCCGGTGGTTGTGGCTAC+TGG 0.281226 6.3:-21236781 MS.gene042577:CDS
TGATGATGATGTTTGTAGTT+TGG 0.317671 6.3:-21237916 MS.gene042577:CDS
GACCAGAGAGATGCTCTTCC+AGG 0.317723 6.3:-21237993 MS.gene042577:CDS
CTGCATTATTTCTCTCCTTT+CGG 0.320408 6.3:+21236822 None:intergenic
ACGGCCTTGTTTGGTCTGTT+AGG 0.323691 6.3:+21237039 None:intergenic
CGTGAAGCAGCTAGCTATTT+GGG 0.331259 6.3:-21237019 MS.gene042577:CDS
CTTTCAACCGATTCACCTAA+TGG 0.337119 6.3:-21239456 MS.gene042577:intron
ATGTTCTGCAGCAGTTTGTC+AGG 0.338280 6.3:+21237651 None:intergenic
AGCGATCCCCGCTGCTTTGA+TGG 0.340236 6.3:-21237586 MS.gene042577:CDS
AAGGAATATGTACATCAAAT+AGG 0.341270 6.3:-21236688 MS.gene042577:CDS
AGCTGCTTCACGGCCTTGTT+TGG 0.341608 6.3:+21237030 None:intergenic
TCCAGCACTGGATGATGATT+TGG 0.358270 6.3:+21237863 None:intergenic
GGTAGAGGGGTTGATCTATT+AGG 0.360776 6.3:-21236751 MS.gene042577:CDS
ACATATTCCTTAATCGAATT+AGG 0.361620 6.3:+21236700 None:intergenic
TCATGTGAAGGGTGTTGTTT+CGG 0.371484 6.3:-21237706 MS.gene042577:CDS
AGAGAAGATGGCAAACTCTA+TGG 0.375037 6.3:-21237094 MS.gene042577:CDS
TCATCGTTTGATGTATGTTT+CGG 0.378578 6.3:-21237481 MS.gene042577:CDS
GTCCGATTCTTTAGAGAAGA+TGG 0.393381 6.3:-21237106 MS.gene042577:CDS
ATTTATGGTCGGCGAGTTCC+CGG 0.396686 6.3:-21236797 MS.gene042577:CDS
GGGTAAGAACATGCTTGTTT+TGG 0.404240 6.3:-21237565 MS.gene042577:CDS
TGATGTCAAGGTGAGTTCTA+AGG 0.405988 6.3:-21238015 MS.gene042577:CDS
AAATCAGGAACGCCGTCTTT+AGG 0.411740 6.3:-21237774 MS.gene042577:CDS
CGGGTGCCGCCGCTGTACCT+CGG 0.418581 6.3:-21236570 MS.gene042577:CDS
TTTAGGAGGTGATCAGGTTC+AGG 0.420816 6.3:-21237757 MS.gene042577:CDS
GAAAGCGATTGAAACTTTGA+TGG 0.426209 6.3:+21239474 None:intergenic
AATGCAATTACAGTTTCAAC+AGG 0.427284 6.3:-21236883 MS.gene042577:CDS
CGGCGAGTTCCCGGTGGTTG+TGG 0.434782 6.3:-21236788 MS.gene042577:CDS
AATAGGAAGGGCATCTAGAA+TGG 0.439220 6.3:-21236671 MS.gene042577:CDS
AACATTACCATTAGGTGAAT+CGG 0.441100 6.3:+21239449 None:intergenic
TGCCATCTTCTCTAAAGAAT+CGG 0.441703 6.3:+21237104 None:intergenic
GTTAAAGCTGTTTCAATTCA+CGG 0.442461 6.3:-21236859 MS.gene042577:CDS
GCCGTCTTTAGGAGGTGATC+AGG 0.459928 6.3:-21237763 MS.gene042577:CDS
GGGATCGCTTGCATTTGAAC+AGG 0.462634 6.3:+21237600 None:intergenic
ACAATGCACTTGGGCTCACC+TGG 0.463822 6.3:+21237975 None:intergenic
AGAGATACTGACAACAAATC+AGG 0.465586 6.3:-21237789 MS.gene042577:CDS
CACGGCTTTGTCACAACCTC+AGG 0.465662 6.3:-21237151 MS.gene042577:CDS
CACCTGGAAGAGCATCTCTC+TGG 0.470474 6.3:+21237991 None:intergenic
TTCTTACCCATCAAAGCAGC+GGG 0.480457 6.3:+21237579 None:intergenic
CACGAGAGAGCTCAGTATAC+AGG 0.484883 6.3:-21237424 MS.gene042577:CDS
TGTGGTTGGTGGTGATGCTA+TGG 0.484916 6.3:-21237349 MS.gene042577:CDS
TAGTTTGGAATGTAAACAAT+CGG 0.486405 6.3:-21237901 MS.gene042577:CDS
ACAAGGAGTTGAATTGATTG+TGG 0.487874 6.3:-21237304 MS.gene042577:CDS
CAAGGAGTTGAATTGATTGT+GGG 0.488532 6.3:-21237303 MS.gene042577:CDS
GGGTGCCGCCGCTGTACCTC+GGG 0.488624 6.3:-21236569 MS.gene042577:CDS
GAAGAGCATGCGAAATTACT+CGG 0.491900 6.3:-21237399 MS.gene042577:CDS
ACTCCAGTAGCCACAACCAC+CGG 0.504693 6.3:+21236778 None:intergenic
GAGCCGACGTATATTACAAC+AGG 0.508711 6.3:+21236931 None:intergenic
TCAGGTCTTGCAGATATTCA+CGG 0.511480 6.3:-21237169 MS.gene042577:CDS
ATATACGTCGGCTCGAGACT+CGG 0.515550 6.3:-21236922 MS.gene042577:CDS
GAAGGGTGTTGTTTCGGCAC+CGG 0.517410 6.3:-21237700 MS.gene042577:CDS
AAAAGATCAACAAGCCGTCC+TGG 0.522470 6.3:+21237276 None:intergenic
CCAAATAGCTAGCTGCTTCA+CGG 0.523412 6.3:+21237020 None:intergenic
TAAAGAATCGGACGTTGTTG+CGG 0.524657 6.3:+21237116 None:intergenic
GAATTGATTGTGGGAACACC+AGG 0.524874 6.3:-21237294 MS.gene042577:CDS
ATAGGAAGGGCATCTAGAAT+GGG 0.525349 6.3:-21236670 MS.gene042577:CDS
GTTAGTCGATGTATTAAAGT+CGG 0.529271 6.3:-21236590 MS.gene042577:CDS
GCGATCCCCGCTGCTTTGAT+GGG 0.530341 6.3:-21237585 MS.gene042577:CDS
AAAACTGCACTTGTGGTTGG+TGG 0.533633 6.3:-21237360 MS.gene042577:CDS
AGACTTTCCTGAACCTGTAT+CGG 0.533821 6.3:+21237542 None:intergenic
CACATATATATTCACCGTAT+CGG 0.534333 6.3:+21237946 None:intergenic
CTGTATACTGAGCTCTCTCG+TGG 0.536245 6.3:+21237425 None:intergenic
TCGGCCACATACAATGCACT+TGG 0.536389 6.3:+21237965 None:intergenic
AATATGTACATCAAATAGGA+AGG 0.538019 6.3:-21236684 MS.gene042577:CDS
CACTTCATCAACAACAAAAG+TGG 0.538859 6.3:+21237221 None:intergenic
TCCAAATCATCATCCAGTGC+TGG 0.539876 6.3:-21237864 MS.gene042577:CDS
CGAACCTAACAGACCAAACA+AGG 0.541101 6.3:-21237043 MS.gene042577:CDS
TAAATGCACTCATCGGCTGC+AGG 0.548168 6.3:+21237816 None:intergenic
TGCATTGTATGTGGCCGATA+CGG 0.555640 6.3:-21237960 MS.gene042577:CDS
CTGCAGAACATAGAGGCAGC+TGG 0.555928 6.3:-21237639 MS.gene042577:CDS
ATGCGAAATTACTCGGGAAA+GGG 0.557733 6.3:-21237392 MS.gene042577:CDS
TTAGTCGATGTATTAAAGTC+GGG 0.558344 6.3:-21236589 MS.gene042577:CDS
GAAACTCGACATTCATGTGA+AGG 0.558611 6.3:-21237718 MS.gene042577:CDS
AAAGCGATTGAAACTTTGAT+GGG 0.561237 6.3:+21239475 None:intergenic
CATGAAGCGAACGACATAGC+CGG 0.561576 6.3:+21237681 None:intergenic
AAGGGCATCTAGAATGGGAG+AGG 0.566272 6.3:-21236665 MS.gene042577:CDS
CTCCAGTAGCCACAACCACC+GGG 0.567240 6.3:+21236779 None:intergenic
TGTTCTGCAGCAGTTTGTCA+GGG 0.575665 6.3:+21237652 None:intergenic
TTTAAAACTGCACTTGTGGT+TGG 0.575676 6.3:-21237363 MS.gene042577:CDS
TGGTCTGTTAGGTTCGCCAA+CGG 0.581517 6.3:+21237050 None:intergenic
TCAGGAACGCCGTCTTTAGG+AGG 0.581955 6.3:-21237771 MS.gene042577:CDS
CATGCGAAATTACTCGGGAA+AGG 0.586359 6.3:-21237393 MS.gene042577:CDS
GCATCTAGAATGGGAGAGGA+AGG 0.588001 6.3:-21236661 MS.gene042577:CDS
AGATGTAGGGAATGATGTCA+AGG 0.589680 6.3:-21238027 MS.gene042577:CDS
GATACAGTGGTTATAACCGT+TGG 0.592053 6.3:-21237066 MS.gene042577:CDS
CAAACTGCTGCAGAACATAG+AGG 0.592770 6.3:-21237646 MS.gene042577:CDS
CAACTTTACCGCATTCAACA+AGG 0.593925 6.3:-21237321 MS.gene042577:CDS
AAGAGCATGCGAAATTACTC+GGG 0.598647 6.3:-21237398 MS.gene042577:CDS
TGGTGGAAGACATCCAGCAC+TGG 0.602023 6.3:+21237851 None:intergenic
ATTCAACTCCTTGTTGAATG+CGG 0.607306 6.3:+21237313 None:intergenic
GCTTGCATTTGAACAGGCGT+AGG 0.608901 6.3:+21237606 None:intergenic
CTCTATGGCAAAAGATACAG+TGG 0.614077 6.3:-21237079 MS.gene042577:CDS
TCTAACATAAATGCACTCAT+CGG 0.620582 6.3:+21237809 None:intergenic
GAGCCCAAGTGCATTGTATG+TGG 0.621540 6.3:-21237969 MS.gene042577:CDS
TGTATACTGAGCTCTCTCGT+GGG 0.623700 6.3:+21237426 None:intergenic
AAACTCGACATTCATGTGAA+GGG 0.624831 6.3:-21237717 MS.gene042577:CDS
GTGGACTGCATGCTTCAAAG+AGG 0.631114 6.3:-21237201 MS.gene042577:CDS
GCGAGAAATCTATGACCGAA+AGG 0.633150 6.3:-21236837 MS.gene042577:CDS
ACCTGATCACCTCCTAAAGA+CGG 0.633787 6.3:+21237762 None:intergenic
TCCTCCAGAGAAAACCCTTG+TGG 0.636938 6.3:+21236525 None:intergenic
GATAAGCTCCCGAGGTACAG+CGG 0.640895 6.3:+21236561 None:intergenic
ATATGTACATCAAATAGGAA+GGG 0.645395 6.3:-21236683 MS.gene042577:CDS
GCGGAATACATTAAGACCTG+AGG 0.646184 6.3:+21237135 None:intergenic
CGGCCACATACAATGCACTT+GGG 0.647710 6.3:+21237966 None:intergenic
CCGACGTATATTACAACAGG+TGG 0.652630 6.3:+21236934 None:intergenic
TCTGCAGCAGTTTGTCAGGG+AGG 0.653253 6.3:+21237655 None:intergenic
GCCGTTTAAAACTGCACTTG+TGG 0.655718 6.3:-21237367 MS.gene042577:CDS
ATAATGCAGTCATTTATGGT+CGG 0.657786 6.3:-21236808 MS.gene042577:CDS
GTTCTTACCCATCAAAGCAG+CGG 0.665952 6.3:+21237578 None:intergenic
GGTGTTAACACCATAGCTAG+AGG 0.666575 6.3:+21237447 None:intergenic
TGATTGTGGGAACACCAGGA+CGG 0.673152 6.3:-21237290 MS.gene042577:CDS
CAATTCACAGTATACCACAA+GGG 0.675999 6.3:-21236539 MS.gene042577:CDS
CCACCTGTTGTAATATACGT+CGG 0.679670 6.3:-21236934 MS.gene042577:CDS
TCAATTCACAGTATACCACA+AGG 0.680822 6.3:-21236540 MS.gene042577:CDS
TGTGAATTGATAAGCTCCCG+AGG 0.687454 6.3:+21236553 None:intergenic
TATGGTCGGCGAGTTCCCGG+TGG 0.702170 6.3:-21236794 MS.gene042577:CDS
AAGCTCCCGAGGTACAGCGG+CGG 0.720985 6.3:+21236564 None:intergenic
TCTTACCCATCAAAGCAGCG+GGG 0.806131 6.3:+21237580 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAACAAGATTAAT+TGG + chr6.3:21237944-21237963 None:intergenic 10.0%
!!! AATTTCATATTTTTTTTTCT+GGG - chr6.3:21236609-21236628 MS.gene042577:CDS 10.0%
!!! AAATCAATTTTAGAATCAAA+AGG + chr6.3:21237101-21237120 None:intergenic 15.0%
!!! CAATTTCATATTTTTTTTTC+TGG - chr6.3:21236608-21236627 MS.gene042577:CDS 15.0%
!!! GTTTATTTATTTTAGATGTA+GGG - chr6.3:21237953-21237972 MS.gene042577:CDS 15.0%
!!! TGTTTATTTATTTTAGATGT+AGG - chr6.3:21237952-21237971 MS.gene042577:CDS 15.0%
!! AATAAATTGTTTATCCAAAC+AGG + chr6.3:21236732-21236751 None:intergenic 20.0%
!! ATAAATTGTTTATCCAAACA+GGG + chr6.3:21236731-21236750 None:intergenic 20.0%
!! TCAATCTTTCTGTTAATTTA+AGG - chr6.3:21237797-21237816 MS.gene042577:CDS 20.0%
!!! AAAACCTTTTTTGGAATTTT+AGG - chr6.3:21237362-21237381 MS.gene042577:CDS 20.0%
!!! AAACCTTTTTTGGAATTTTA+GGG - chr6.3:21237363-21237382 MS.gene042577:CDS 20.0%
!!! ATAAGTTGATTATTCAAACA+TGG + chr6.3:21236960-21236979 None:intergenic 20.0%
!!! TCTTATTTGAGCTTATTTAT+TGG - chr6.3:21237515-21237534 MS.gene042577:CDS 20.0%
!!! TTCTTTTCTATTTTCTTTTG+CGG - chr6.3:21237466-21237485 MS.gene042577:CDS 20.0%
!!! TTTGTTTTTCTAGTGAATTT+TGG - chr6.3:21237829-21237848 MS.gene042577:CDS 20.0%
! AAGGAATATGTACATCAAAT+AGG - chr6.3:21239305-21239324 MS.gene042577:intron 25.0%
! AATATGTACATCAAATAGGA+AGG - chr6.3:21239309-21239328 MS.gene042577:intron 25.0%
! ACATATTCCTTAATCGAATT+AGG + chr6.3:21239296-21239315 None:intergenic 25.0%
! AGAAATAATGCAGTCATTTA+TGG - chr6.3:21239181-21239200 MS.gene042577:intron 25.0%
! ATATGTACATCAAATAGGAA+GGG - chr6.3:21239310-21239329 MS.gene042577:intron 25.0%
! ATTCCAAAAGTAGACAATTT+AGG + chr6.3:21236926-21236945 None:intergenic 25.0%
! CTCATCATTTACAAAAACTA+TGG + chr6.3:21239363-21239382 None:intergenic 25.0%
! CTTTGCAAACTTATGAAAAT+TGG - chr6.3:21237256-21237275 MS.gene042577:CDS 25.0%
! TGAAGAATAGATTTCAATAC+AGG + chr6.3:21236583-21236602 None:intergenic 25.0%
!! ACAATTTTGTGTATGTTTGT+TGG + chr6.3:21237201-21237220 None:intergenic 25.0%
!! TAGTTTGGAATGTAAACAAT+CGG - chr6.3:21238092-21238111 MS.gene042577:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+TGG - chr6.3:21237720-21237739 MS.gene042577:CDS 25.0%
!!! AGTAATGAGAAAACCTTTTT+TGG - chr6.3:21237353-21237372 MS.gene042577:CDS 25.0%
!!! GTCTACTTTTGGAATTATAA+TGG - chr6.3:21236931-21236950 MS.gene042577:CDS 25.0%
AAAGCGATTGAAACTTTGAT+GGG + chr6.3:21236521-21236540 None:intergenic 30.0%
AACATTACCATTAGGTGAAT+CGG + chr6.3:21236547-21236566 None:intergenic 30.0%
AATGCAATTACAGTTTCAAC+AGG - chr6.3:21239110-21239129 MS.gene042577:intron 30.0%
AGTGTTAACAATACTCATGT+AGG - chr6.3:21236684-21236703 MS.gene042577:CDS 30.0%
ATAATGCAGTCATTTATGGT+CGG - chr6.3:21239185-21239204 MS.gene042577:intron 30.0%
ATCAACTAGAATCAATTCAG+AGG + chr6.3:21237062-21237081 None:intergenic 30.0%
ATGTTGTATTGTCGTTATTG+CGG - chr6.3:21237871-21237890 MS.gene042577:CDS 30.0%
CACATATATATTCACCGTAT+CGG + chr6.3:21238050-21238069 None:intergenic 30.0%
CTCAAATAAGACAATCCAAA+GGG + chr6.3:21237506-21237525 None:intergenic 30.0%
CTTAGAGAACTTATGATAGT+TGG - chr6.3:21236853-21236872 MS.gene042577:CDS 30.0%
GAATTAGGAACATTACCATT+AGG + chr6.3:21236555-21236574 None:intergenic 30.0%
GTTAGTCGATGTATTAAAGT+CGG - chr6.3:21239403-21239422 MS.gene042577:intron 30.0%
TCAAATAAGACAATCCAAAG+GGG + chr6.3:21237505-21237524 None:intergenic 30.0%
TCAATACAGGAAGATGAATT+AGG + chr6.3:21236570-21236589 None:intergenic 30.0%
TCATCGTTTGATGTATGTTT+CGG - chr6.3:21238512-21238531 MS.gene042577:intron 30.0%
TCTAACATAAATGCACTCAT+CGG + chr6.3:21238187-21238206 None:intergenic 30.0%
TTAGTCGATGTATTAAAGTC+GGG - chr6.3:21239404-21239423 MS.gene042577:intron 30.0%
! GTTAAAGCTGTTTCAATTCA+CGG - chr6.3:21239134-21239153 MS.gene042577:intron 30.0%
! TCTCCTAAATTGTCTACTTT+TGG - chr6.3:21236920-21236939 MS.gene042577:CDS 30.0%
! TGATGATGATGTTTGTAGTT+TGG - chr6.3:21238077-21238096 MS.gene042577:intron 30.0%
! TGTTTTCATATAAGCTATCC+TGG + chr6.3:21237631-21237650 None:intergenic 30.0%
!! TTTTGATTATAGACCCTGTT+TGG - chr6.3:21236715-21236734 MS.gene042577:CDS 30.0%
AAACTCGACATTCATGTGAA+GGG - chr6.3:21238276-21238295 MS.gene042577:intron 35.0%
ACAAGGAGTTGAATTGATTG+TGG - chr6.3:21238689-21238708 MS.gene042577:intron 35.0%
AGAAAGATTGATCACTCTAC+TGG + chr6.3:21237788-21237807 None:intergenic 35.0%
AGAGATACTGACAACAAATC+AGG - chr6.3:21238204-21238223 MS.gene042577:intron 35.0%
ATTCAACTCCTTGTTGAATG+CGG + chr6.3:21238683-21238702 None:intergenic 35.0%
CAAGGAGTTGAATTGATTGT+GGG - chr6.3:21238690-21238709 MS.gene042577:intron 35.0%
CAATTCACAGTATACCACAA+GGG - chr6.3:21239454-21239473 MS.gene042577:intron 35.0%
CACTTCATCAACAACAAAAG+TGG + chr6.3:21238775-21238794 None:intergenic 35.0%
CAGGTGGCTTAAAATGTTTA+TGG + chr6.3:21239046-21239065 None:intergenic 35.0%
CTTGCACAACGAGAAATTAT+TGG + chr6.3:21238492-21238511 None:intergenic 35.0%
GAAAGCGATTGAAACTTTGA+TGG + chr6.3:21236522-21236541 None:intergenic 35.0%
GAGCCCTAAAATTCCAAAAA+AGG + chr6.3:21237369-21237388 None:intergenic 35.0%
GAGCTTATGGAAATAGCTTA+TGG - chr6.3:21237565-21237584 MS.gene042577:CDS 35.0%
GCTCAAATAAGACAATCCAA+AGG + chr6.3:21237507-21237526 None:intergenic 35.0%
TAAGAAAAAGCCTCTAGCTA+TGG - chr6.3:21238536-21238555 MS.gene042577:intron 35.0%
TAGCTTATGGCATGTTCATA+AGG - chr6.3:21237578-21237597 MS.gene042577:CDS 35.0%
TATGAAAATTGGCTGAACAC+AGG - chr6.3:21237267-21237286 MS.gene042577:CDS 35.0%
TCAATTCACAGTATACCACA+AGG - chr6.3:21239453-21239472 MS.gene042577:intron 35.0%
! CTGCATTATTTCTCTCCTTT+CGG + chr6.3:21239174-21239193 None:intergenic 35.0%
! TGCCATCTTCTCTAAAGAAT+CGG + chr6.3:21238892-21238911 None:intergenic 35.0%
! TTTAAAACTGCACTTGTGGT+TGG - chr6.3:21238630-21238649 MS.gene042577:intron 35.0%
!! GTTTTTGAAAACAACCCCTT+TGG - chr6.3:21237488-21237507 MS.gene042577:CDS 35.0%
!! TGATTCTAGTTGATAGCAGA+CGG - chr6.3:21237068-21237087 MS.gene042577:CDS 35.0%
!!! ACCACAAGTGCAGTTTTAAA+CGG + chr6.3:21238630-21238649 None:intergenic 35.0%
!!! CACTTTTGTTGTTGATGAAG+TGG - chr6.3:21238773-21238792 MS.gene042577:intron 35.0%
!!! GTGAATTTTGGTGTCATGAT+TGG - chr6.3:21237841-21237860 MS.gene042577:CDS 35.0%
AGACTTTCCTGAACCTGTAT+CGG + chr6.3:21238454-21238473 None:intergenic 40.0%
AGAGAAGATGGCAAACTCTA+TGG - chr6.3:21238899-21238918 MS.gene042577:intron 40.0%
AGATGTAGGGAATGATGTCA+AGG - chr6.3:21237966-21237985 MS.gene042577:CDS 40.0%
ATAAGCACCAGTGTGGTTAT+TGG - chr6.3:21237539-21237558 MS.gene042577:CDS 40.0%
ATGCGAAATTACTCGGGAAA+GGG - chr6.3:21238601-21238620 MS.gene042577:intron 40.0%
CAACTTTACCGCATTCAACA+AGG - chr6.3:21238672-21238691 MS.gene042577:intron 40.0%
CCACCTGTTGTAATATACGT+CGG - chr6.3:21239059-21239078 MS.gene042577:intron 40.0%
CGACATGCCTAATTCGATTA+AGG - chr6.3:21239286-21239305 MS.gene042577:intron 40.0%
CTCTATGGCAAAAGATACAG+TGG - chr6.3:21238914-21238933 MS.gene042577:intron 40.0%
CTTATGATCATAAGCTCTCC+AGG - chr6.3:21237610-21237629 MS.gene042577:CDS 40.0%
GAAACTCGACATTCATGTGA+AGG - chr6.3:21238275-21238294 MS.gene042577:intron 40.0%
GATACAGTGGTTATAACCGT+TGG - chr6.3:21238927-21238946 MS.gene042577:intron 40.0%
GCAACAGTAACAACCATGTT+TGG + chr6.3:21237736-21237755 None:intergenic 40.0%
TAAAGAATCGGACGTTGTTG+CGG + chr6.3:21238880-21238899 None:intergenic 40.0%
TCAGGTCTTGCAGATATTCA+CGG - chr6.3:21238824-21238843 MS.gene042577:intron 40.0%
TGATGTCAAGGTGAGTTCTA+AGG - chr6.3:21237978-21237997 MS.gene042577:CDS 40.0%
TGGTTATTGGAGAGAGCTTA+TGG - chr6.3:21237552-21237571 MS.gene042577:CDS 40.0%
! AAGAGCATGCGAAATTACTC+GGG - chr6.3:21238595-21238614 MS.gene042577:intron 40.0%
! CTTTCAACCGATTCACCTAA+TGG - chr6.3:21236537-21236556 MS.gene042577:CDS 40.0%
! GAAGAGCATGCGAAATTACT+CGG - chr6.3:21238594-21238613 MS.gene042577:intron 40.0%
! GGGTAAGAACATGCTTGTTT+TGG - chr6.3:21238428-21238447 MS.gene042577:intron 40.0%
! GTCCGATTCTTTAGAGAAGA+TGG - chr6.3:21238887-21238906 MS.gene042577:intron 40.0%
! TATACCACAAGGGTTTTCTC+TGG - chr6.3:21239464-21239483 MS.gene042577:CDS 40.0%
!! AATAGGAAGGGCATCTAGAA+TGG - chr6.3:21239322-21239341 MS.gene042577:intron 40.0%
!! ATAGGAAGGGCATCTAGAAT+GGG - chr6.3:21239323-21239342 MS.gene042577:intron 40.0%
!! GTGTATGTTTGTTGGTTCTG+TGG + chr6.3:21237193-21237212 None:intergenic 40.0%
!! TCAAAGAGGTTTTCGAGATC+AGG - chr6.3:21238806-21238825 MS.gene042577:intron 40.0%
!! TCATGTGAAGGGTGTTGTTT+CGG - chr6.3:21238287-21238306 MS.gene042577:intron 40.0%
AAAACTGCACTTGTGGTTGG+TGG - chr6.3:21238633-21238652 MS.gene042577:intron 45.0%
AAAAGATCAACAAGCCGTCC+TGG + chr6.3:21238720-21238739 None:intergenic 45.0%
AAATCAGGAACGCCGTCTTT+AGG - chr6.3:21238219-21238238 MS.gene042577:intron 45.0%
ACCTGATCACCTCCTAAAGA+CGG + chr6.3:21238234-21238253 None:intergenic 45.0%
ATGTTCTGCAGCAGTTTGTC+AGG + chr6.3:21238345-21238364 None:intergenic 45.0%
CAAACTGCTGCAGAACATAG+AGG - chr6.3:21238347-21238366 MS.gene042577:intron 45.0%
CATGCGAAATTACTCGGGAA+AGG - chr6.3:21238600-21238619 MS.gene042577:intron 45.0%
CCAAATAGCTAGCTGCTTCA+CGG + chr6.3:21238976-21238995 None:intergenic 45.0%
CCGACGTATATTACAACAGG+TGG + chr6.3:21239062-21239081 None:intergenic 45.0%
CGAACCTAACAGACCAAACA+AGG - chr6.3:21238950-21238969 MS.gene042577:intron 45.0%
CGTGAAGCAGCTAGCTATTT+GGG - chr6.3:21238974-21238993 MS.gene042577:intron 45.0%
GAATTGATTGTGGGAACACC+AGG - chr6.3:21238699-21238718 MS.gene042577:intron 45.0%
GAGCCGACGTATATTACAAC+AGG + chr6.3:21239065-21239084 None:intergenic 45.0%
GCGAGAAATCTATGACCGAA+AGG - chr6.3:21239156-21239175 MS.gene042577:intron 45.0%
GCGGAATACATTAAGACCTG+AGG + chr6.3:21238861-21238880 None:intergenic 45.0%
GGTGTTAACACCATAGCTAG+AGG + chr6.3:21238549-21238568 None:intergenic 45.0%
GTCGTTATTGCGGAAAATCG+TGG - chr6.3:21237881-21237900 MS.gene042577:CDS 45.0%
GTTCTTACCCATCAAAGCAG+CGG + chr6.3:21238418-21238437 None:intergenic 45.0%
TATTGGCATAAGCACCAGTG+TGG - chr6.3:21237532-21237551 MS.gene042577:CDS 45.0%
TCCAAATCATCATCCAGTGC+TGG - chr6.3:21238129-21238148 MS.gene042577:intron 45.0%
TGCATTGTATGTGGCCGATA+CGG - chr6.3:21238033-21238052 MS.gene042577:intron 45.0%
TGTATACTGAGCTCTCTCGT+GGG + chr6.3:21238570-21238589 None:intergenic 45.0%
TGTGAATTGATAAGCTCCCG+AGG + chr6.3:21239443-21239462 None:intergenic 45.0%
TGTTCTGCAGCAGTTTGTCA+GGG + chr6.3:21238344-21238363 None:intergenic 45.0%
TTCTTACCCATCAAAGCAGC+GGG + chr6.3:21238417-21238436 None:intergenic 45.0%
TTTAGGAGGTGATCAGGTTC+AGG - chr6.3:21238236-21238255 MS.gene042577:intron 45.0%
! GCCGTTTAAAACTGCACTTG+TGG - chr6.3:21238626-21238645 MS.gene042577:intron 45.0%
! GGTAGAGGGGTTGATCTATT+AGG - chr6.3:21239242-21239261 MS.gene042577:intron 45.0%
! GTTGTGGCTACTGGAGTTTT+GGG - chr6.3:21239221-21239240 MS.gene042577:intron 45.0%
! TCCAGCACTGGATGATGATT+TGG + chr6.3:21238133-21238152 None:intergenic 45.0%
!! AAGGGTTTTCTCTGGAGGAA+AGG - chr6.3:21239472-21239491 MS.gene042577:CDS 45.0%
!! AGGGTTTTCTCTGGAGGAAA+GGG - chr6.3:21239473-21239492 MS.gene042577:CDS 45.0%
!!! ATGCTTGTTTTGGCCGATAC+AGG - chr6.3:21238438-21238457 MS.gene042577:intron 45.0%
!!! CTACTGGAGTTTTGGGTAGA+GGG - chr6.3:21239228-21239247 MS.gene042577:intron 45.0%
!!! TACTGGAGTTTTGGGTAGAG+GGG - chr6.3:21239229-21239248 MS.gene042577:intron 45.0%
ATTTATGGTCGGCGAGTTCC+CGG - chr6.3:21239196-21239215 MS.gene042577:intron 50.0%
CACGAGAGAGCTCAGTATAC+AGG - chr6.3:21238569-21238588 MS.gene042577:intron 50.0%
CATGAAGCGAACGACATAGC+CGG + chr6.3:21238315-21238334 None:intergenic 50.0%
CCGTGAAGCAGCTAGCTATT+TGG - chr6.3:21238973-21238992 MS.gene042577:intron 50.0%
CGGCCACATACAATGCACTT+GGG + chr6.3:21238030-21238049 None:intergenic 50.0%
CTGTATACTGAGCTCTCTCG+TGG + chr6.3:21238571-21238590 None:intergenic 50.0%
GAGCCCAAGTGCATTGTATG+TGG - chr6.3:21238024-21238043 MS.gene042577:CDS 50.0%
GCTCTCTCCAATAACCACAC+TGG + chr6.3:21237549-21237568 None:intergenic 50.0%
GCTTGCATTTGAACAGGCGT+AGG + chr6.3:21238390-21238409 None:intergenic 50.0%
GGGATCGCTTGCATTTGAAC+AGG + chr6.3:21238396-21238415 None:intergenic 50.0%
TCCTCCAGAGAAAACCCTTG+TGG + chr6.3:21239471-21239490 None:intergenic 50.0%
TCGGCCACATACAATGCACT+TGG + chr6.3:21238031-21238050 None:intergenic 50.0%
TCTTACCCATCAAAGCAGCG+GGG + chr6.3:21238416-21238435 None:intergenic 50.0%
TGATCACTCTACTGGCAGTC+TGG + chr6.3:21237780-21237799 None:intergenic 50.0%
TGATTGTGGGAACACCAGGA+CGG - chr6.3:21238703-21238722 MS.gene042577:intron 50.0%
TGGTCTGTTAGGTTCGCCAA+CGG + chr6.3:21238946-21238965 None:intergenic 50.0%
! ACCACAAGGGTTTTCTCTGG+AGG - chr6.3:21239467-21239486 MS.gene042577:CDS 50.0%
! GCATCTAGAATGGGAGAGGA+AGG - chr6.3:21239332-21239351 MS.gene042577:intron 50.0%
! GGTTGTGGCTACTGGAGTTT+TGG - chr6.3:21239220-21239239 MS.gene042577:intron 50.0%
! GTGGACTGCATGCTTCAAAG+AGG - chr6.3:21238792-21238811 MS.gene042577:intron 50.0%
! TAAATGCACTCATCGGCTGC+AGG + chr6.3:21238180-21238199 None:intergenic 50.0%
! TCGGCTGCAGGTAGCTTTTT+AGG + chr6.3:21238168-21238187 None:intergenic 50.0%
!! AAGGGCATCTAGAATGGGAG+AGG - chr6.3:21239328-21239347 MS.gene042577:intron 50.0%
!! ACGGCCTTGTTTGGTCTGTT+AGG + chr6.3:21238957-21238976 None:intergenic 50.0%
!! ATATACGTCGGCTCGAGACT+CGG - chr6.3:21239071-21239090 MS.gene042577:intron 50.0%
!! GCTGCAGGTAGCTTTTTAGG+TGG + chr6.3:21238165-21238184 None:intergenic 50.0%
!! GTTTTGGCCGATACAGGTTC+AGG - chr6.3:21238444-21238463 MS.gene042577:intron 50.0%
!! TGTGGTTGGTGGTGATGCTA+TGG - chr6.3:21238644-21238663 MS.gene042577:intron 50.0%
!!! GCAGGTAGCTTTTTAGGTGG+TGG + chr6.3:21238162-21238181 None:intergenic 50.0%
!!! GCTACTGGAGTTTTGGGTAG+AGG - chr6.3:21239227-21239246 MS.gene042577:intron 50.0%
ACAATGCACTTGGGCTCACC+TGG + chr6.3:21238021-21238040 None:intergenic 55.0%
ACTCCAGTAGCCACAACCAC+CGG + chr6.3:21239218-21239237 None:intergenic 55.0%
CTGCAGAACATAGAGGCAGC+TGG - chr6.3:21238354-21238373 MS.gene042577:intron 55.0%
GACCAGAGAGATGCTCTTCC+AGG - chr6.3:21238000-21238019 MS.gene042577:CDS 55.0%
GATAAGCTCCCGAGGTACAG+CGG + chr6.3:21239435-21239454 None:intergenic 55.0%
TCAGGAACGCCGTCTTTAGG+AGG - chr6.3:21238222-21238241 MS.gene042577:intron 55.0%
TCTGCAGCAGTTTGTCAGGG+AGG + chr6.3:21238341-21238360 None:intergenic 55.0%
TGGTGGAAGACATCCAGCAC+TGG + chr6.3:21238145-21238164 None:intergenic 55.0%
! CACGGCTTTGTCACAACCTC+AGG - chr6.3:21238842-21238861 MS.gene042577:intron 55.0%
! GCCGTCTTTAGGAGGTGATC+AGG - chr6.3:21238230-21238249 MS.gene042577:intron 55.0%
!! AGCTGCTTCACGGCCTTGTT+TGG + chr6.3:21238966-21238985 None:intergenic 55.0%
!! CACCTGGAAGAGCATCTCTC+TGG + chr6.3:21238005-21238024 None:intergenic 55.0%
!! GAAGGGTGTTGTTTCGGCAC+CGG - chr6.3:21238293-21238312 MS.gene042577:intron 55.0%
CTCCAGTAGCCACAACCACC+GGG + chr6.3:21239217-21239236 None:intergenic 60.0%
TTCCCGGTGGTTGTGGCTAC+TGG - chr6.3:21239212-21239231 MS.gene042577:intron 60.0%
! AGCGATCCCCGCTGCTTTGA+TGG - chr6.3:21238407-21238426 MS.gene042577:intron 60.0%
! GCGATCCCCGCTGCTTTGAT+GGG - chr6.3:21238408-21238427 MS.gene042577:intron 60.0%
AAGCTCCCGAGGTACAGCGG+CGG + chr6.3:21239432-21239451 None:intergenic 65.0%
TATGGTCGGCGAGTTCCCGG+TGG - chr6.3:21239199-21239218 MS.gene042577:intron 65.0%
CGGCGAGTTCCCGGTGGTTG+TGG - chr6.3:21239205-21239224 MS.gene042577:intron 70.0%
! CGGGTGCCGCCGCTGTACCT+CGG - chr6.3:21239423-21239442 MS.gene042577:intron 75.0%
! GGGTGCCGCCGCTGTACCTC+GGG - chr6.3:21239424-21239443 MS.gene042577:intron 75.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 21236495 21239520 21236495 ID=MS.gene042577
chr6.3 mRNA 21236495 21239520 21236495 ID=MS.gene042577.t1;Parent=MS.gene042577
chr6.3 exon 21239457 21239520 21239457 ID=MS.gene042577.t1.exon1;Parent=MS.gene042577.t1
chr6.3 CDS 21239457 21239520 21239457 ID=cds.MS.gene042577.t1;Parent=MS.gene042577.t1
chr6.3 exon 21236495 21238047 21236495 ID=MS.gene042577.t1.exon2;Parent=MS.gene042577.t1
chr6.3 CDS 21236495 21238047 21236495 ID=cds.MS.gene042577.t1;Parent=MS.gene042577.t1
Gene Sequence

>MS.gene042577

ATGGTTGATGATTCTAACGATGACCCATCAAAGTTTCAATCGCTTTCAACCGATTCACCTAATGGTAATGTTCCTAATTCATCTTCCTGTATTGAAATCTATTCTTCAATATTCAATTTCATATTTTTTTTTCTGGGTATTGTTGATTGAATATATTTCAATTGCTGTTTTCAAATTTTTGTATAATTCAGTGTTAACAATACTCATGTAGGTTTTTTGTTTTTGATTATAGACCCTGTTTGGATAAACAATTTATTGCACAAGCACTTATCATGATAAGCACTTATGTATAAGCTATTTCTATAGCAAATTGTAAAATTGTTGTTTGTATAAGTTATAAGCTATATTTATAAGTTATCTTAGAGAACTTATGATAGTTGGTTGAAAACAACTTATGAAGAATGTCATAAGTTGTTTTTGTAAGTTCTCCTAAATTGTCTACTTTTGGAATTATAATGGAGACCATGTTTGAATAATCAACTTATTTGTAGCTTATATGATAAGCGCTTATGTATAAGTCATTTCTATAGCAGATGATTACTGAGCAGATGTATAACTTATTTGCCTCTGAATTGATTCTAGTTGATAGCAGACGGATTTGTACCTTTTGATTCTAAAATTGATTTTATACTCTAAATTTTATTGTTCAATTCACTTTTAGACAGAATCAGTTCACTCAAAATCAATTCTTGTCACCACAGAACCAACAAACATACACAAAATTGTCTTCTTATAAGTTATAAGCTATTTTCATAAGTTATCTTTGCAAACTTATGAAAATTGGCTGAACACAGGTTATAAAGAATGTTATCAGCTGTTTTCATAAGTTCTTCTAAATAGTTTCACAAAACTTATGCTAGTAATGAGAAAACCTTTTTTGGAATTTTAGGGCTCGTATAATTTTTGAGTATTCTGTTTTCATTTTCACTTTAGCTAATAAAGTTGATAGAATAGAATATAGATTCAGTAGTTTCTTTTCTATTTTCTTTTGCGGTTTTTGAAAACAACCCCTTTGGATTGTCTTATTTGAGCTTATTTATTGGCATAAGCACCAGTGTGGTTATTGGAGAGAGCTTATGGAAATAGCTTATGGCATGTTCATAAGGTATTTGTAGCTTATGATCATAAGCTCTCCAGGATAGCTTATATGAAAACAATTTGACTTTATTCTATCTTTTGTTATAGAAATAGCTTATACATAAACATTTATATGATAAGCACTTAATTAAGTTGTTTATCCAAACATGGTTGTTACTGTTGCTGTTGTATTGCACATTCAGTGCCAGACTGCCAGTAGAGTGATCAATCTTTCTGTTAATTTAAGGAAGAAGTTCTTTGTTTTTCTAGTGAATTTTGGTGTCATGATTGGTTGCGATATGTTGTATTGTCGTTATTGCGGAAAATCGTGGACAAGTTGCGATATGTTAAGTCTCTCAAAATACGAAGCCAATTAATCTTGTTTATTTATTTTAGATGTAGGGAATGATGTCAAGGTGAGTTCTAAGGACCAGAGAGATGCTCTTCCAGGTGAGCCCAAGTGCATTGTATGTGGCCGATACGGTGAATATATATGTGATGAAACTGATGATGATGTTTGTAGTTTGGAATGTAAACAATCGGTACTATCTCGAATTTCCAAATCATCATCCAGTGCTGGATGTCTTCCACCACCTAAAAAGCTACCTGCAGCCGATGAGTGCATTTATGTTAGAGATACTGACAACAAATCAGGAACGCCGTCTTTAGGAGGTGATCAGGTTCAGGCTGAATTGCTTAGAAAGAAACTCGACATTCATGTGAAGGGTGTTGTTTCGGCACCGGCTATGTCGTTCGCTTCATGTAACCTCCCTGACAAACTGCTGCAGAACATAGAGGCAGCTGGTTATGAGATGCCTACGCCTGTTCAAATGCAAGCGATCCCCGCTGCTTTGATGGGTAAGAACATGCTTGTTTTGGCCGATACAGGTTCAGGAAAGTCTGCTTCGTTTCTTATTCCAATAATTTCTCGTTGTGCAAGTCATCGTTTGATGTATGTTTCGGATAAGAAAAAGCCTCTAGCTATGGTGTTAACACCCACGAGAGAGCTCAGTATACAGGTTGAAGAGCATGCGAAATTACTCGGGAAAGGGTTGCCGTTTAAAACTGCACTTGTGGTTGGTGGTGATGCTATGGCTAGACAACTTTACCGCATTCAACAAGGAGTTGAATTGATTGTGGGAACACCAGGACGGCTTGTTGATCTTTTAACGAAAAATGAAATTGATTTAGACGACGTGACCACTTTTGTTGTTGATGAAGTGGACTGCATGCTTCAAAGAGGTTTTCGAGATCAGGTCTTGCAGATATTCACGGCTTTGTCACAACCTCAGGTCTTAATGTATTCCGCAACAACGTCCGATTCTTTAGAGAAGATGGCAAACTCTATGGCAAAAGATACAGTGGTTATAACCGTTGGCGAACCTAACAGACCAAACAAGGCCGTGAAGCAGCTAGCTATTTGGGTCGAGTCGAAACAAAAAAAGCAGAAATTGTTTGATATTTTAAAAAGCCATAAACATTTTAAGCCACCTGTTGTAATATACGTCGGCTCGAGACTCGGAGCAGATCTACTTGCAAATGCAATTACAGTTTCAACAGGAGTTAAAGCTGTTTCAATTCACGGCGAGAAATCTATGACCGAAAGGAGAGAAATAATGCAGTCATTTATGGTCGGCGAGTTCCCGGTGGTTGTGGCTACTGGAGTTTTGGGTAGAGGGGTTGATCTATTAGGCGTAAGACAAGTTATTGTATTCGACATGCCTAATTCGATTAAGGAATATGTACATCAAATAGGAAGGGCATCTAGAATGGGAGAGGAAGGTCAAGCCATAGTTTTTGTAAATGATGAGAATAAGAATGTTTTTGCAGAGTTAGTCGATGTATTAAAGTCGGGTGCCGCCGCTGTACCTCGGGAGCTTATCAATTCACAGTATACCACAAGGGTTTTCTCTGGAGGAAAGGGTTCAAAGAAAAGAAAATATAGATGA
Protein sequence

>MS.gene042577.t1

MVDDSNDDPSKFQSLSTDSPNDVGNDVKVSSKDQRDALPGEPKCIVCGRYGEYICDETDDDVCSLECKQSVLSRISKSSSSAGCLPPPKKLPAADECIYVRDTDNKSGTPSLGGDQVQAELLRKKLDIHVKGVVSAPAMSFASCNLPDKLLQNIEAAGYEMPTPVQMQAIPAALMGKNMLVLADTGSGKSASFLIPIISRCASHRLMYVSDKKKPLAMVLTPTRELSIQVEEHAKLLGKGLPFKTALVVGGDAMARQLYRIQQGVELIVGTPGRLVDLLTKNEIDLDDVTTFVVDEVDCMLQRGFRDQVLQIFTALSQPQVLMYSATTSDSLEKMANSMAKDTVVITVGEPNRPNKAVKQLAIWVESKQKKQKLFDILKSHKHFKPPVVIYVGSRLGADLLANAITVSTGVKAVSIHGEKSMTERREIMQSFMVGEFPVVVATGVLGRGVDLLGVRQVIVFDMPNSIKEYVHQIGRASRMGEEGQAIVFVNDENKNVFAELVDVLKSGAAAVPRELINSQYTTRVFSGGKGSKKRKYR