AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025141.t1 MTR_2g039960 100.000 413 0 0 1 413 1 413 0.0 850
MS.gene025141.t1 MTR_4g125970 93.462 413 27 0 1 413 18 430 0.0 803
MS.gene025141.t1 MTR_4g125970 93.462 413 27 0 1 413 1 413 0.0 803
MS.gene025141.t1 MTR_2g099620 92.978 413 29 0 1 413 1 413 0.0 800
MS.gene025141.t1 MTR_8g018400 92.736 413 30 0 1 413 1 413 0.0 797
MS.gene025141.t1 MTR_2g099620 92.405 395 30 0 19 413 3 397 0.0 757
MS.gene025141.t1 MTR_2g438110 65.952 373 127 0 41 413 34 406 0.0 523
MS.gene025141.t1 MTR_1g046570 60.660 394 118 5 56 413 6 398 7.65e-162 461
MS.gene025141.t1 MTR_8g018370 65.029 346 94 4 71 413 3 324 4.84e-151 431
MS.gene025141.t1 MTR_7g076820 40.872 367 215 2 40 406 149 513 1.34e-90 283
MS.gene025141.t1 MTR_7g076800 40.054 367 218 2 40 406 140 504 8.73e-89 278
MS.gene025141.t1 MTR_7g076830 40.054 367 218 2 40 406 142 506 1.08e-88 278
MS.gene025141.t1 MTR_3g107420 40.761 368 212 6 47 409 53 419 7.33e-80 253
MS.gene025141.t1 MTR_1g012420 39.674 368 216 6 47 409 53 419 1.50e-79 252
MS.gene025141.t1 MTR_4g010565 39.946 368 215 6 47 409 130 496 9.91e-78 249
MS.gene025141.t1 MTR_3g467550 35.638 376 218 5 40 396 116 486 1.40e-68 227
MS.gene025141.t1 MTR_1g061970 36.543 405 217 13 41 411 80 478 2.54e-68 224
MS.gene025141.t1 MTR_3g467550 35.501 369 214 5 47 396 92 455 1.70e-66 221
MS.gene025141.t1 MTR_4g049910 34.896 384 224 9 41 405 55 431 2.12e-65 216
MS.gene025141.t1 MTR_8g018350 63.905 169 47 5 252 413 15 176 1.71e-64 204
MS.gene025141.t1 MTR_3g449250 36.170 376 227 7 45 411 94 465 3.23e-64 215
MS.gene025141.t1 MTR_3g449250 35.200 375 232 5 45 411 94 465 5.53e-64 215
MS.gene025141.t1 MTR_2g100230 34.615 364 224 3 41 394 96 455 4.50e-63 211
MS.gene025141.t1 MTR_4g134170 33.415 410 228 7 17 387 254 657 1.78e-62 214
MS.gene025141.t1 MTR_5g090120 34.501 371 230 6 45 406 85 451 7.97e-62 210
MS.gene025141.t1 MTR_6g082080 33.423 371 243 3 41 408 10 379 1.01e-61 206
MS.gene025141.t1 MTR_7g056830 33.423 371 243 3 41 408 10 379 2.38e-61 205
MS.gene025141.t1 MTR_0915s0010 38.516 283 172 2 54 336 2 282 2.39e-61 200
MS.gene025141.t1 MTR_6g005320 35.230 369 205 6 47 387 255 617 1.52e-60 208
MS.gene025141.t1 MTR_3g083690 33.846 390 235 8 40 407 144 532 7.60e-60 205
MS.gene025141.t1 MTR_8g018330 58.929 168 58 4 252 413 15 177 7.78e-60 192
MS.gene025141.t1 MTR_7g109720 34.254 362 229 6 41 394 232 592 1.31e-59 207
MS.gene025141.t1 MTR_4g105100 33.333 363 236 2 41 397 202 564 5.74e-58 200
MS.gene025141.t1 MTR_4g114120 33.673 392 231 6 42 407 141 529 3.46e-57 199
MS.gene025141.t1 MTR_2g066050 34.167 360 232 3 41 395 53 412 4.77e-57 195
MS.gene025141.t1 MTR_4g079320 31.989 372 245 4 42 407 20 389 7.64e-57 199
MS.gene025141.t1 MTR_1g026830 30.848 389 250 8 41 413 147 532 7.61e-56 194
MS.gene025141.t1 MTR_3g467550 34.226 336 199 5 40 357 116 447 2.21e-55 189
MS.gene025141.t1 MTR_1g054630 30.698 430 264 8 12 407 111 540 3.95e-55 192
MS.gene025141.t1 MTR_1g095810 30.848 389 250 8 41 413 145 530 6.16e-55 191
MS.gene025141.t1 MTR_2g007000 33.590 390 231 7 41 412 504 883 7.85e-55 196
MS.gene025141.t1 MTR_3g049990 33.880 366 230 5 40 396 151 513 7.33e-54 191
MS.gene025141.t1 MTR_2g032630 33.149 362 235 4 41 396 496 856 1.40e-53 192
MS.gene025141.t1 MTR_6g005550 31.579 380 249 5 41 412 165 541 1.46e-53 189
MS.gene025141.t1 MTR_5g027840 33.681 383 234 7 36 403 96 473 1.05e-52 184
MS.gene025141.t1 MTR_2g041140 32.637 383 242 7 27 396 124 503 5.71e-49 177
MS.gene025141.t1 MTR_6g033070 32.143 364 235 6 41 394 140 501 6.23e-49 174
MS.gene025141.t1 MTR_2g055850 30.328 366 236 9 40 391 90 450 3.43e-44 162
MS.gene025141.t1 MTR_4g112340 30.447 358 227 10 50 391 106 457 7.03e-43 158
MS.gene025141.t1 MTR_7g100820 28.900 391 251 7 34 412 130 505 3.09e-42 157
MS.gene025141.t1 MTR_6g056080 30.484 351 217 6 45 380 115 453 1.28e-41 156
MS.gene025141.t1 MTR_2g063080 26.077 418 244 7 41 396 109 523 6.71e-41 152
MS.gene025141.t1 MTR_3g071620 28.919 370 233 8 47 388 22 389 1.29e-39 149
MS.gene025141.t1 MTR_4g105100 31.392 309 206 2 41 343 202 510 3.18e-39 147
MS.gene025141.t1 MTR_7g057250 25.850 441 241 10 39 399 161 595 3.94e-38 147
MS.gene025141.t1 MTR_8g027780 27.456 397 240 10 42 406 107 487 1.36e-37 144
MS.gene025141.t1 MTR_8g027775 28.278 389 247 10 42 406 103 483 1.71e-37 143
MS.gene025141.t1 MTR_8g097000 27.865 384 255 7 42 406 289 669 2.86e-37 144
MS.gene025141.t1 MTR_1g102410 28.997 369 219 6 47 380 50 410 5.65e-37 142
MS.gene025141.t1 MTR_8g027770 27.456 397 235 10 42 406 202 577 6.09e-37 142
MS.gene025141.t1 MTR_1g083130 27.139 409 243 8 41 397 26 431 4.16e-36 140
MS.gene025141.t1 MTR_3g107420 42.188 192 108 3 47 235 53 244 4.38e-35 132
MS.gene025141.t1 MTR_6g039840 28.272 382 242 10 41 396 110 485 1.14e-34 136
MS.gene025141.t1 MTR_4g068990 28.198 344 224 9 68 395 102 438 1.62e-33 133
MS.gene025141.t1 MTR_5g092560 27.371 369 241 9 68 413 102 466 1.07e-32 130
MS.gene025141.t1 MTR_4g112320 30.249 281 183 8 117 391 2 275 5.34e-32 125
MS.gene025141.t1 MTR_4g059460 25.901 444 246 11 41 403 44 485 7.77e-31 124
MS.gene025141.t1 MTR_5g069330 27.151 372 251 7 36 396 2 364 3.64e-29 118
MS.gene025141.t1 MTR_5g069330 27.248 367 247 7 41 396 3 360 7.03e-29 117
MS.gene025141.t1 MTR_8g027780 27.536 345 201 10 42 352 107 436 1.50e-27 114
MS.gene025141.t1 MTR_4g131750 26.854 391 239 12 49 401 90 471 1.65e-27 114
MS.gene025141.t1 MTR_0427s0010 32.068 237 111 8 198 411 3 212 1.05e-26 107
MS.gene025141.t1 MTR_6g039840 29.276 304 192 8 110 396 12 309 1.16e-26 112
MS.gene025141.t1 MTR_3g118120 26.683 401 240 12 24 383 120 507 1.16e-25 109
MS.gene025141.t1 MTR_4g077370 36.139 202 123 3 25 223 237 435 1.99e-25 107
MS.gene025141.t1 MTR_4g077370 34.197 193 95 4 41 223 2 172 4.80e-18 86.3
MS.gene025141.t1 MTR_5g077690 25.661 378 212 12 79 389 71 446 3.64e-24 104
MS.gene025141.t1 MTR_4g068990 28.636 220 146 5 183 395 12 227 4.38e-20 92.8
MS.gene025141.t1 MTR_4g459350 31.050 219 123 7 41 232 41 258 5.46e-20 89.7
MS.gene025141.t1 MTR_1g022305 24.779 339 185 9 63 395 26 300 5.91e-20 92.0
MS.gene025141.t1 MTR_8g032230 38.235 136 83 1 41 175 10 145 2.30e-19 85.1
MS.gene025141.t1 MTR_5g092560 28.638 213 146 4 183 390 12 223 6.93e-19 89.0
MS.gene025141.t1 MTR_2g043360 31.492 181 115 4 41 212 41 221 2.50e-18 87.4
MS.gene025141.t1 MTR_2g043360 35.052 97 62 1 310 406 389 484 2.50e-14 75.1
MS.gene025141.t1 MTR_8g042690 23.861 373 250 11 53 404 30 389 4.84e-17 83.2
MS.gene025141.t1 MTR_2g043360 35.052 97 62 1 310 406 271 366 2.33e-14 75.1
MS.gene025141.t1 MTR_1g009617 31.683 101 67 1 313 413 178 276 3.58e-12 67.8
MS.gene025141.t1 MTR_1g023070 29.703 101 69 1 313 413 37 135 4.25e-12 64.7
MS.gene025141.t1 MTR_1g023070 29.703 101 69 1 313 413 37 135 2.29e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025141.t1 AT3G13920 91.546 414 32 2 1 413 1 412 0.0 782
MS.gene025141.t1 AT1G54270 91.304 414 33 2 1 413 1 412 0.0 781
MS.gene025141.t1 AT3G13920 90.338 414 32 3 1 413 1 407 0.0 765
MS.gene025141.t1 AT1G72730 88.164 414 48 1 1 413 1 414 0.0 765
MS.gene025141.t1 AT1G54270 90.097 414 33 3 1 413 1 407 0.0 763
MS.gene025141.t1 AT3G13920 93.490 384 25 0 30 413 19 402 0.0 749
MS.gene025141.t1 AT3G13920 91.858 393 29 2 1 392 5 395 0.0 743
MS.gene025141.t1 AT3G13920 91.858 393 29 2 1 392 1 391 0.0 743
MS.gene025141.t1 AT3G19760 65.416 373 129 0 41 413 36 408 0.0 518
MS.gene025141.t1 AT1G51380 59.621 369 149 0 41 409 23 391 9.55e-166 471
MS.gene025141.t1 AT1G51380 59.524 252 102 0 41 292 23 274 9.15e-110 324
MS.gene025141.t1 AT3G61240 40.000 370 220 2 40 409 124 491 8.13e-92 286
MS.gene025141.t1 AT3G61240 40.000 370 220 2 40 409 124 491 8.13e-92 286
MS.gene025141.t1 AT4G00660 39.189 370 223 2 40 409 131 498 3.30e-90 282
MS.gene025141.t1 AT4G00660 39.189 370 223 2 40 409 131 498 3.30e-90 282
MS.gene025141.t1 AT2G45810 40.217 368 218 2 40 407 154 519 4.22e-90 282
MS.gene025141.t1 AT1G51380 56.872 211 91 0 41 251 23 233 1.62e-83 258
MS.gene025141.t1 AT1G51380 56.872 211 91 0 41 251 23 233 3.22e-83 257
MS.gene025141.t1 AT5G11170 39.946 368 215 6 47 409 53 419 8.13e-79 250
MS.gene025141.t1 AT5G11200 39.946 368 215 6 47 409 53 419 8.13e-79 250
MS.gene025141.t1 AT5G11170 39.466 337 198 6 78 409 1 336 5.39e-71 227
MS.gene025141.t1 AT5G11200 35.941 409 215 7 47 409 53 460 1.72e-70 229
MS.gene025141.t1 AT3G53110 35.338 399 232 11 33 407 81 477 7.75e-70 229
MS.gene025141.t1 AT5G26742 34.762 420 245 10 9 413 80 485 9.71e-66 223
MS.gene025141.t1 AT5G26742 34.762 420 245 10 9 413 80 485 9.85e-66 223
MS.gene025141.t1 AT5G26742 37.709 358 203 9 71 413 40 392 1.96e-65 221
MS.gene025141.t1 AT2G33730 34.217 415 238 7 17 397 281 694 3.48e-65 221
MS.gene025141.t1 AT3G22310 35.372 376 230 6 45 411 121 492 4.51e-65 218
MS.gene025141.t1 AT3G22310 35.372 376 230 6 45 411 121 492 6.01e-65 218
MS.gene025141.t1 AT3G22330 35.372 376 230 6 45 411 109 480 8.83e-65 218
MS.gene025141.t1 AT5G63120 34.656 378 241 2 42 413 167 544 1.03e-63 215
MS.gene025141.t1 AT1G55150 34.146 369 234 3 41 403 100 465 1.42e-63 212
MS.gene025141.t1 AT1G55150 34.146 369 234 3 41 403 125 490 2.10e-63 213
MS.gene025141.t1 AT1G77030 31.989 372 245 4 42 407 30 399 1.86e-59 207
MS.gene025141.t1 AT5G60990 32.225 391 251 3 36 413 5 394 2.32e-58 197
MS.gene025141.t1 AT3G01540 35.526 380 234 8 41 412 159 535 3.20e-58 201
MS.gene025141.t1 AT5G14610 33.598 378 244 4 41 412 170 546 3.25e-58 201
MS.gene025141.t1 AT3G01540 35.526 380 234 8 41 412 159 535 3.29e-58 201
MS.gene025141.t1 AT3G01540 35.526 380 234 8 41 412 159 535 3.29e-58 201
MS.gene025141.t1 AT3G01540 35.526 380 234 8 41 412 159 535 3.29e-58 201
MS.gene025141.t1 AT5G14610 33.598 378 244 4 41 412 162 538 3.77e-58 201
MS.gene025141.t1 AT5G14610 33.598 378 244 4 41 412 162 538 3.99e-58 201
MS.gene025141.t1 AT1G16280 33.795 361 234 2 41 396 59 419 9.22e-58 197
MS.gene025141.t1 AT1G20920 33.777 376 239 5 46 412 197 571 1.82e-57 202
MS.gene025141.t1 AT3G58570 33.083 399 240 7 33 406 136 532 3.64e-57 198
MS.gene025141.t1 AT2G47330 33.594 384 238 8 41 412 229 607 3.88e-57 200
MS.gene025141.t1 AT2G42520 32.542 421 250 10 16 406 129 545 4.18e-57 198
MS.gene025141.t1 AT2G42520 32.542 421 250 10 16 406 129 545 4.18e-57 198
MS.gene025141.t1 AT2G42520 32.542 421 250 10 16 406 129 545 4.18e-57 198
MS.gene025141.t1 AT3G58510 32.216 388 239 7 40 406 151 535 6.47e-57 197
MS.gene025141.t1 AT3G58510 32.216 388 239 7 40 406 151 535 6.47e-57 197
MS.gene025141.t1 AT3G58510 32.216 388 239 7 40 406 151 535 6.47e-57 197
MS.gene025141.t1 AT4G16630 34.400 375 237 4 31 396 155 529 1.35e-56 199
MS.gene025141.t1 AT3G06480 33.508 382 243 5 39 412 434 812 1.78e-56 201
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT1G20920 34.127 378 235 6 46 412 535 909 8.48e-56 199
MS.gene025141.t1 AT3G02065 32.877 365 232 5 41 394 111 473 1.66e-54 188
MS.gene025141.t1 AT3G02065 32.877 365 232 5 41 394 111 473 1.66e-54 188
MS.gene025141.t1 AT5G14610 32.418 364 239 4 55 412 45 407 3.30e-54 188
MS.gene025141.t1 AT5G14610 32.418 364 239 4 55 412 45 407 3.30e-54 188
MS.gene025141.t1 AT5G51280 30.670 388 250 7 41 412 147 531 7.80e-54 188
MS.gene025141.t1 AT4G33370 31.701 388 246 8 41 412 98 482 8.31e-54 187
MS.gene025141.t1 AT5G14610 32.787 366 239 4 53 412 242 606 7.05e-53 188
MS.gene025141.t1 AT1G28180 33.799 358 214 7 63 410 5 349 5.73e-52 178
MS.gene025141.t1 AT1G31970 32.713 376 235 7 41 403 117 487 6.27e-51 179
MS.gene025141.t1 AT3G18600 31.842 380 249 7 41 412 90 467 1.34e-50 179
MS.gene025141.t1 AT3G02065 33.233 331 208 5 75 394 8 336 2.28e-49 171
MS.gene025141.t1 AT1G12770 28.429 401 225 6 41 381 111 509 3.69e-49 175
MS.gene025141.t1 AT1G12770 28.429 401 225 6 41 381 111 509 3.69e-49 175
MS.gene025141.t1 AT3G09720 30.541 370 251 4 46 412 147 513 1.72e-48 173
MS.gene025141.t1 AT3G09720 30.541 370 251 4 46 412 147 513 1.72e-48 173
MS.gene025141.t1 AT3G09620 32.440 373 213 7 46 409 403 745 5.15e-48 176
MS.gene025141.t1 AT3G09620 32.440 373 213 7 46 409 402 744 6.66e-48 176
MS.gene025141.t1 AT5G65900 31.367 373 245 6 41 404 155 525 1.94e-46 169
MS.gene025141.t1 AT5G62190 31.989 372 225 9 55 407 111 473 3.09e-44 163
MS.gene025141.t1 AT5G63120 34.091 308 197 2 42 343 167 474 6.21e-44 160
MS.gene025141.t1 AT5G63120 34.091 308 197 2 42 343 167 474 7.08e-44 159
MS.gene025141.t1 AT5G63120 34.091 308 197 2 42 343 167 474 7.08e-44 159
MS.gene025141.t1 AT5G63120 34.091 308 197 2 42 343 167 474 7.08e-44 159
MS.gene025141.t1 AT5G05450 29.973 367 230 8 47 388 23 387 1.82e-42 157
MS.gene025141.t1 AT1G71370 30.028 353 229 8 47 383 23 373 3.53e-41 153
MS.gene025141.t1 AT5G14610 31.611 329 218 4 41 363 170 497 3.80e-41 152
MS.gene025141.t1 AT5G63630 30.027 373 242 7 42 395 56 428 1.12e-39 149
MS.gene025141.t1 AT3G06480 31.677 322 209 5 39 352 434 752 1.31e-39 151
MS.gene025141.t1 AT5G63630 30.027 373 242 7 42 395 250 622 1.41e-38 147
MS.gene025141.t1 AT5G08620 29.948 384 228 9 42 395 82 454 1.64e-38 146
MS.gene025141.t1 AT5G08610 29.765 383 230 9 42 395 384 756 1.02e-37 145
MS.gene025141.t1 AT2G07750 27.295 403 252 11 35 407 368 759 1.22e-37 145
MS.gene025141.t1 AT1G63250 27.047 403 253 10 35 407 321 712 1.52e-35 139
MS.gene025141.t1 AT5G11200 38.938 226 124 5 47 267 53 269 1.70e-35 137
MS.gene025141.t1 AT5G11200 38.506 174 104 3 238 409 306 478 1.94e-30 122
MS.gene025141.t1 AT4G34910 26.034 411 247 8 41 397 47 454 2.01e-34 135
MS.gene025141.t1 AT5G54910 27.778 396 261 11 17 396 49 435 6.36e-34 134
MS.gene025141.t1 AT2G40700 25.676 444 249 12 41 403 31 474 1.35e-32 130
MS.gene025141.t1 AT5G19210 27.456 397 228 12 49 402 85 464 1.57e-32 128
MS.gene025141.t1 AT3G16840 26.424 439 236 12 39 400 189 617 5.76e-32 129
MS.gene025141.t1 AT3G16840 26.424 439 236 12 39 400 190 618 5.76e-32 129
MS.gene025141.t1 AT4G09730 25.515 388 253 8 37 396 109 488 5.77e-31 125
MS.gene025141.t1 AT3G06980 28.684 380 229 14 41 383 375 749 1.27e-27 115
MS.gene025141.t1 AT1G71280 28.115 313 183 10 47 345 24 308 1.92e-27 112
MS.gene025141.t1 AT4G15850 25.648 386 211 13 79 394 74 453 1.24e-26 112
MS.gene025141.t1 AT1G71280 28.435 313 182 10 47 345 24 308 1.53e-26 111
MS.gene025141.t1 AT5G19210 26.006 323 179 12 123 402 2 307 5.55e-18 84.7
MS.gene025141.t1 AT5G19210 26.006 323 179 12 123 402 2 307 5.55e-18 84.7
MS.gene025141.t1 AT1G28180 37.398 123 60 3 63 177 5 118 1.49e-13 67.4

Find 91 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAATGTCCCTAGTATTT+TGG 0.210988 2.4:-50088283 None:intergenic
ACATCAAGACCCTTGATAAA+TGG 0.268068 2.4:-50086829 None:intergenic
TTTGATAACTACCGATCTTC+TGG 0.320168 2.4:+50088337 MS.gene025141:CDS
TCAACACATTGTAGAACTTC+TGG 0.325013 2.4:-50088523 None:intergenic
TGATGTTCAGCAAGTGTCTT+TGG 0.336264 2.4:+50088370 MS.gene025141:CDS
CTCAGAGGCATTTACGCATA+TGG 0.350883 2.4:+50086225 MS.gene025141:CDS
CTGTGATCTTTATGAGACTT+TGG 0.355425 2.4:+50088151 MS.gene025141:CDS
CAACAGGCTCAGTCTGGAAC+AGG 0.390696 2.4:+50086856 MS.gene025141:CDS
ATCATGCGTGAATTCCGTTC+TGG 0.390799 2.4:+50088302 MS.gene025141:CDS
GCCATCAGCTATTCAGCAAC+GGG 0.393795 2.4:+50086798 MS.gene025141:CDS
ACTCCGACTTGAATCTTAGA+TGG 0.408502 2.4:-50087972 None:intergenic
AAGACTGCAACCTTCTGCTC+TGG 0.409029 2.4:+50086880 MS.gene025141:CDS
TTACATCAAAATGTTCGTAT+TGG 0.414807 2.4:+50087155 MS.gene025141:CDS
GGGAATAGTTCCATTTATCA+AGG 0.444746 2.4:+50086819 MS.gene025141:CDS
CATGTTGTTGTTGGTACCCC+TGG 0.445958 2.4:+50087090 MS.gene025141:CDS
AGCATGTCAAATACACGTCC+AGG 0.452941 2.4:-50087108 None:intergenic
CTTGTGATCTCAAGTGCCTC+AGG 0.456300 2.4:-50088014 None:intergenic
ATTGGTCGTAGTGGAAGGTT+TGG 0.459303 2.4:+50088440 MS.gene025141:CDS
GGGTCTTGATGTTATACAAC+AGG 0.460845 2.4:+50086840 MS.gene025141:CDS
GAGACATGGACCAAAATACT+AGG 0.463229 2.4:+50088273 MS.gene025141:CDS
GTTATACAACAGGCTCAGTC+TGG 0.464551 2.4:+50086850 MS.gene025141:CDS
TTACTTCCTGCAGTCATGGC+AGG 0.465723 2.4:+50085913 None:intergenic
GATAGTTCTCAGGCTGTGTT+GGG 0.470642 2.4:-50088409 None:intergenic
CGTGCACTTGGAGATTATCT+AGG 0.472296 2.4:+50086997 MS.gene025141:CDS
AGCGGTGTTCATGTTGTTGT+TGG 0.477024 2.4:+50087081 MS.gene025141:CDS
CGAATGCCAAGCCTTGGTCT+TGG 0.479480 2.4:+50086933 MS.gene025141:CDS
CAAGACCAAGGCTTGGCATT+CGG 0.481084 2.4:-50086932 None:intergenic
GTTCTACAATGTGTTGATTG+AGG 0.485517 2.4:+50088529 MS.gene025141:CDS
GCGTGATGAGCTCACCTTGG+AGG 0.488852 2.4:+50088073 MS.gene025141:CDS
TCACAGAAGTTCAGCAACAT+TGG 0.492163 2.4:-50088561 None:intergenic
GAGAAGGTCATGCGTGCACT+TGG 0.495619 2.4:+50086985 MS.gene025141:CDS
CAGCCAGTCAACCTTTCGTC+TGG 0.496367 2.4:-50088204 None:intergenic
ACACCAGACGAAAGGTTGAC+TGG 0.497320 2.4:+50088201 MS.gene025141:CDS
GCATCAAACTGTGAACCCTC+TGG 0.509050 2.4:-50085943 None:intergenic
AGACTGCAACCTTCTGCTCT+GGG 0.510787 2.4:+50086881 MS.gene025141:CDS
CCCCGTTGCTGAATAGCTGA+TGG 0.513832 2.4:-50086799 None:intergenic
GAATCTTAGATGGCAACAGC+TGG 0.517992 2.4:-50087962 None:intergenic
CTCTGCTACAATGCCTCCTG+AGG 0.521626 2.4:+50087998 MS.gene025141:CDS
GAGAACTATCTCCATCGTAT+TGG 0.523557 2.4:+50088422 MS.gene025141:CDS
AGATAGTTCTCAGGCTGTGT+TGG 0.525373 2.4:-50088410 None:intergenic
ATCGTATTGGTCGTAGTGGA+AGG 0.529668 2.4:+50088435 MS.gene025141:CDS
GCGCTGATCTTCACGAACAC+TGG 0.540854 2.4:-50087049 None:intergenic
CTCCATCGTATTGGTCGTAG+TGG 0.541717 2.4:+50088431 MS.gene025141:CDS
GTCGTAGTGGAAGGTTTGGA+AGG 0.547854 2.4:+50088444 MS.gene025141:CDS
CGTGATGAGCTCACCTTGGA+GGG 0.552554 2.4:+50088074 MS.gene025141:CDS
CATGGCAGGCGTTGCACCAG+AGG 0.552942 2.4:+50085927 None:intergenic
CTGCAAAATCCCAGAGCAGA+AGG 0.553669 2.4:-50086890 None:intergenic
CAGACTCACAACTGATGCAC+AGG 0.557617 2.4:+50086137 MS.gene025141:intron
ATACGATGGAGATAGTTCTC+AGG 0.557654 2.4:-50088419 None:intergenic
CTCAGCAACACACGGAGACA+TGG 0.558453 2.4:+50088259 MS.gene025141:CDS
AGTGGAAGGTTTGGAAGGAA+AGG 0.560979 2.4:+50088449 MS.gene025141:CDS
CTGAGGCACTTGAGATCACA+AGG 0.563772 2.4:+50088015 MS.gene025141:CDS
TTATGTCAACGTTGATAAGG+AGG 0.566533 2.4:+50088109 MS.gene025141:CDS
TGTGACCGAATGCCAAGCCT+TGG 0.571337 2.4:+50086927 MS.gene025141:CDS
TCAACGTTGATAAGGAGGAA+TGG 0.577711 2.4:+50088114 MS.gene025141:CDS
CAAAATGTTCGTATTGGACG+AGG 0.578200 2.4:+50087161 MS.gene025141:CDS
CGCTTCACAAGAATCCTCAC+AGG 0.579557 2.4:-50088053 None:intergenic
TTGCTGAGACAGTGTGGTCA+CGG 0.580925 2.4:-50088244 None:intergenic
ATGGCAGGCGTTGCACCAGA+GGG 0.587343 2.4:+50085928 MS.gene025141:CDS
GGAATAGTTCCATTTATCAA+GGG 0.589236 2.4:+50086820 MS.gene025141:CDS
AGAAGTTCAGCAACATTGGA+AGG 0.591345 2.4:-50088557 None:intergenic
AGCCATCAGCTATTCAGCAA+CGG 0.592072 2.4:+50086797 MS.gene025141:CDS
CTTCTTCACATCTTATGATG+AGG 0.599921 2.4:+50086161 MS.gene025141:CDS
GCATGTCAAATACACGTCCA+GGG 0.601935 2.4:-50087107 None:intergenic
GCTGATGAGATGCTTTCTCG+TGG 0.610128 2.4:+50087183 MS.gene025141:CDS
ATCGGTAGTTATCAAAACAC+GGG 0.610300 2.4:-50088330 None:intergenic
CACACTGTCTCAGCAACACA+CGG 0.611599 2.4:+50088251 MS.gene025141:CDS
TGGAGATTATCTAGGTGTGA+AGG 0.612268 2.4:+50087005 MS.gene025141:CDS
TAGGTGCCAAGACCAAGGCT+TGG 0.617896 2.4:-50086939 None:intergenic
GATCGGTAGTTATCAAAACA+CGG 0.623883 2.4:-50088331 None:intergenic
GATCAGCGCATTCTATCAAG+CGG 0.624396 2.4:+50087063 MS.gene025141:CDS
AGACATGGACCAAAATACTA+GGG 0.628384 2.4:+50088274 MS.gene025141:CDS
AATACCACGAGCCAGAAGAT+CGG 0.631247 2.4:-50088348 None:intergenic
AAGGTGCATGCTTGTGTCGG+AGG 0.634488 2.4:+50087024 MS.gene025141:CDS
GCTTGCTCAGCAGATTGAGA+AGG 0.634804 2.4:+50086969 MS.gene025141:CDS
GTGAAGGTGCATGCTTGTGT+CGG 0.636750 2.4:+50087021 MS.gene025141:CDS
TTCCACTACGACCAATACGA+TGG 0.638034 2.4:-50088433 None:intergenic
GTGATCTCAAGTGCCTCAGG+AGG 0.642550 2.4:-50088011 None:intergenic
ACTACCGATCTTCTGGCTCG+TGG 0.651230 2.4:+50088344 MS.gene025141:CDS
AACAGGCTCAGTCTGGAACA+GGG 0.652293 2.4:+50086857 MS.gene025141:CDS
ACGAGTAGGTGCCAAGACCA+AGG 0.655523 2.4:-50086944 None:intergenic
TGCTGAGCAAGCTCACGAGT+AGG 0.660506 2.4:-50086958 None:intergenic
AAACTGCTTGATACCCTCCA+AGG 0.661421 2.4:-50088087 None:intergenic
TTGCCATCTAAGATTCAAGT+CGG 0.662032 2.4:+50087969 MS.gene025141:CDS
GAAGCGTGATGAGCTCACCT+TGG 0.671691 2.4:+50088070 MS.gene025141:CDS
CATGTCAAATACACGTCCAG+GGG 0.696747 2.4:-50087106 None:intergenic
TTGCAAGAGAATCTTCTCAG+AGG 0.702805 2.4:+50086210 MS.gene025141:CDS
AAACACGGGAAGATCCAGAA+CGG 0.712720 2.4:-50088316 None:intergenic
CGTGTGTTGCTGAGACAGTG+TGG 0.721187 2.4:-50088250 None:intergenic
AGTTCATGAACAAACCTGTG+AGG 0.726622 2.4:+50088039 MS.gene025141:CDS
CCATCAGCTATTCAGCAACG+GGG 0.741503 2.4:+50086799 MS.gene025141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTTAAAAAACAAATTA+AGG + chr2.4:50087371-50087390 MS.gene025141:intron 10.0%
!!! TTCTTTATAATTTTTGAAAT+TGG + chr2.4:50086465-50086484 MS.gene025141:intron 10.0%
!! AAGAATTAAAATGTTATTGT+TGG + chr2.4:50086341-50086360 MS.gene025141:intron 15.0%
!! AGAATTAAAATGTTATTGTT+GGG + chr2.4:50086342-50086361 MS.gene025141:intron 15.0%
!! CAATAATATTACTATTTACT+GGG - chr2.4:50087765-50087784 None:intergenic 15.0%
!! TGGTTAAAAAAATTAAAGAT+TGG - chr2.4:50086291-50086310 None:intergenic 15.0%
!! TTTCTTAATGGAAAATATTA+AGG + chr2.4:50087250-50087269 MS.gene025141:intron 15.0%
!!! AATATATTTCATTTTGAAAC+AGG - chr2.4:50087566-50087585 None:intergenic 15.0%
!!! ATTTCTAAAAAATTAATCCT+CGG - chr2.4:50087703-50087722 None:intergenic 15.0%
!!! TTTAATTTTTTTAACCAGAT+GGG + chr2.4:50086294-50086313 MS.gene025141:intron 15.0%
!! AAATAAAAACAAAGCTATGA+CGG - chr2.4:50086011-50086030 None:intergenic 20.0%
!! ACAAATCAAACTAGTTATTA+AGG - chr2.4:50086761-50086780 None:intergenic 20.0%
!! CAGATACAATACAAAATTTA+AGG - chr2.4:50086378-50086397 None:intergenic 20.0%
!! CCAATAATATTACTATTTAC+TGG - chr2.4:50087766-50087785 None:intergenic 20.0%
!! CCAGTAAATAGTAATATTAT+TGG + chr2.4:50087763-50087782 MS.gene025141:intron 20.0%
!! GTTAAAAAACAAATTAAGGT+GGG + chr2.4:50087375-50087394 MS.gene025141:intron 20.0%
!! TAGATCTGAAAATAAAATGA+AGG - chr2.4:50087933-50087952 None:intergenic 20.0%
!! TGTTAAAAAACAAATTAAGG+TGG + chr2.4:50087374-50087393 MS.gene025141:intron 20.0%
!! TTATGTATCAATAAAAGACT+CGG - chr2.4:50086720-50086739 None:intergenic 20.0%
!!! AAGTTTTTATAGTAAATGAG+AGG - chr2.4:50086618-50086637 None:intergenic 20.0%
!!! ATGCATACATTAATTCTTTT+AGG - chr2.4:50087903-50087922 None:intergenic 20.0%
!!! CACTTTCTAAAATTTCTTAA+TGG + chr2.4:50087238-50087257 MS.gene025141:intron 20.0%
!!! CTTAAATTTTGTATTGTATC+TGG + chr2.4:50086376-50086395 MS.gene025141:intron 20.0%
!!! CTTTAATTTTTTTAACCAGA+TGG + chr2.4:50086293-50086312 MS.gene025141:intron 20.0%
!!! TTTAGAAATTGTTTGAAACA+TGG + chr2.4:50087714-50087733 MS.gene025141:intron 20.0%
! AAAAGTAGAAATCGAACTTA+TGG - chr2.4:50086507-50086526 None:intergenic 25.0%
! AAATAGGAAGCAAAACAAAA+CGG - chr2.4:50086434-50086453 None:intergenic 25.0%
! AAATTAAAGATTGGTACAGA+AGG - chr2.4:50086282-50086301 None:intergenic 25.0%
! AATAAAAACAAAGCTATGAC+GGG - chr2.4:50086010-50086029 None:intergenic 25.0%
! ATGGGAATCTTAAATTTCTA+TGG - chr2.4:50087468-50087487 None:intergenic 25.0%
! CAGGTTAAAAAATTATTCTC+TGG - chr2.4:50087547-50087566 None:intergenic 25.0%
! TCAAAATCTTCAAGATTGAA+AGG - chr2.4:50087665-50087684 None:intergenic 25.0%
! TTACATCAAAATGTTCGTAT+TGG + chr2.4:50087155-50087174 MS.gene025141:CDS 25.0%
!! GAAAGTGTACAAAAATTAGA+AGG - chr2.4:50087225-50087244 None:intergenic 25.0%
!!! ACTAGTTTGATTTGTGTTTT+AGG + chr2.4:50086766-50086785 MS.gene025141:intron 25.0%
!!! TATGATAGTTTTGATGCTAT+GGG + chr2.4:50086186-50086205 MS.gene025141:CDS 25.0%
!!! TTCTTGTTTTTGTCTAACTT+CGG + chr2.4:50086092-50086111 MS.gene025141:intron 25.0%
!!! TTTTAGGACAATAAATCAGT+AGG - chr2.4:50087887-50087906 None:intergenic 25.0%
AAAGTCATACAACATTAGCA+AGG - chr2.4:50086659-50086678 None:intergenic 30.0%
AAGAAACAACCGTGATAAAT+AGG - chr2.4:50086450-50086469 None:intergenic 30.0%
AATAGGAAGCAAAACAAAAC+GGG - chr2.4:50086433-50086452 None:intergenic 30.0%
ACAGAAACAAGAACAATCTT+GGG - chr2.4:50086542-50086561 None:intergenic 30.0%
AGGATATACTACGTAGAAAA+AGG - chr2.4:50086598-50086617 None:intergenic 30.0%
ATAAAAACAAAGCTATGACG+GGG - chr2.4:50086009-50086028 None:intergenic 30.0%
ATAGTACAAGACTGTGTTTA+AGG - chr2.4:50087793-50087812 None:intergenic 30.0%
GGAATAGTTCCATTTATCAA+GGG + chr2.4:50086820-50086839 MS.gene025141:CDS 30.0%
GTTAACAAAAATGACACTCT+GGG - chr2.4:50088183-50088202 None:intergenic 30.0%
TACAGAAACAAGAACAATCT+TGG - chr2.4:50086543-50086562 None:intergenic 30.0%
TAGTACAAGACTGTGTTTAA+GGG - chr2.4:50087792-50087811 None:intergenic 30.0%
TGTTAACAAAAATGACACTC+TGG - chr2.4:50088184-50088203 None:intergenic 30.0%
TTGTGTAGTAAGGATAAAAG+TGG + chr2.4:50087401-50087420 MS.gene025141:intron 30.0%
! AAACACAGTCTTGTACTATT+AGG + chr2.4:50087794-50087813 MS.gene025141:intron 30.0%
! AGTCTTGTACTATTAGGATT+AGG + chr2.4:50087800-50087819 MS.gene025141:intron 30.0%
! GTTTTATGTCAACGTTGATA+AGG + chr2.4:50088106-50088125 MS.gene025141:CDS 30.0%
! GTTTTGCTTCCTATTTATCA+CGG + chr2.4:50086438-50086457 MS.gene025141:intron 30.0%
!! ACGAACATTTTGATGTAATC+TGG - chr2.4:50087153-50087172 None:intergenic 30.0%
!! TGATAATGTCCCTAGTATTT+TGG - chr2.4:50088286-50088305 None:intergenic 30.0%
!!! CTATGATAGTTTTGATGCTA+TGG + chr2.4:50086185-50086204 MS.gene025141:CDS 30.0%
!!! GATCTTTATTTTACAAACCG+AGG + chr2.4:50087683-50087702 MS.gene025141:intron 30.0%
!!! TCTTGTTTTTGTCTAACTTC+GGG + chr2.4:50086093-50086112 MS.gene025141:intron 30.0%
AAATCTTCAAGATAGCTGAG+AGG - chr2.4:50087852-50087871 None:intergenic 35.0%
AACGGGTATATTTCACAGTA+TGG - chr2.4:50086416-50086435 None:intergenic 35.0%
ACATCAAGACCCTTGATAAA+TGG - chr2.4:50086832-50086851 None:intergenic 35.0%
AGACATGGACCAAAATACTA+GGG + chr2.4:50088274-50088293 MS.gene025141:CDS 35.0%
ATCGGTAGTTATCAAAACAC+GGG - chr2.4:50088333-50088352 None:intergenic 35.0%
CTGTGATCTTTATGAGACTT+TGG + chr2.4:50088151-50088170 MS.gene025141:CDS 35.0%
CTTCTTCACATCTTATGATG+AGG + chr2.4:50086161-50086180 MS.gene025141:CDS 35.0%
GATAAGAACAATGTTCCTAG+AGG - chr2.4:50087320-50087339 None:intergenic 35.0%
GATCGGTAGTTATCAAAACA+CGG - chr2.4:50088334-50088353 None:intergenic 35.0%
GGGAATAGTTCCATTTATCA+AGG + chr2.4:50086819-50086838 MS.gene025141:CDS 35.0%
GGTATATTTCACAGTATGGA+AGG - chr2.4:50086412-50086431 None:intergenic 35.0%
GTACTAGTAATCACATTCCT+AGG + chr2.4:50087272-50087291 MS.gene025141:intron 35.0%
TAAGTTCAAGCTCGAGAAAT+GGG - chr2.4:50087486-50087505 None:intergenic 35.0%
TCAACACATTGTAGAACTTC+TGG - chr2.4:50088526-50088545 None:intergenic 35.0%
TCGGTAGCAAAACTAAAATG+TGG + chr2.4:50087630-50087649 MS.gene025141:intron 35.0%
! TTATGTCAACGTTGATAAGG+AGG + chr2.4:50088109-50088128 MS.gene025141:CDS 35.0%
! TTGCCATCTAAGATTCAAGT+CGG + chr2.4:50087969-50087988 MS.gene025141:CDS 35.0%
! TTTGATAACTACCGATCTTC+TGG + chr2.4:50088337-50088356 MS.gene025141:CDS 35.0%
! TTTTGTTAACACCAGACGAA+AGG + chr2.4:50088193-50088212 MS.gene025141:CDS 35.0%
!! GTTCTACAATGTGTTGATTG+AGG + chr2.4:50088529-50088548 MS.gene025141:CDS 35.0%
!!! TTCTCGTGGTTTTAAAGATC+AGG + chr2.4:50087197-50087216 MS.gene025141:CDS 35.0%
AGAAGTTCAGCAACATTGGA+AGG - chr2.4:50088560-50088579 None:intergenic 40.0%
AGCACGAAAAAAATCCCATC+TGG - chr2.4:50086311-50086330 None:intergenic 40.0%
AGGTGGGAAATTGTGTAGTA+AGG + chr2.4:50087391-50087410 MS.gene025141:intron 40.0%
AGTTCATGAACAAACCTGTG+AGG + chr2.4:50088039-50088058 MS.gene025141:CDS 40.0%
ATACGATGGAGATAGTTCTC+AGG - chr2.4:50088422-50088441 None:intergenic 40.0%
ATGTTATTGTTGGGCTCTGT+TGG + chr2.4:50086351-50086370 MS.gene025141:intron 40.0%
CAAAATGTTCGTATTGGACG+AGG + chr2.4:50087161-50087180 MS.gene025141:CDS 40.0%
CTAAGTTCAAGCTCGAGAAA+TGG - chr2.4:50087487-50087506 None:intergenic 40.0%
GAAACAAACCCTACTACTGT+CGG + chr2.4:50087611-50087630 MS.gene025141:intron 40.0%
GAGAACTATCTCCATCGTAT+TGG + chr2.4:50088422-50088441 MS.gene025141:CDS 40.0%
GAGACATGGACCAAAATACT+AGG + chr2.4:50088273-50088292 MS.gene025141:CDS 40.0%
GGGTCTTGATGTTATACAAC+AGG + chr2.4:50086840-50086859 MS.gene025141:CDS 40.0%
GTCATACAACATTAGCAAGG+TGG - chr2.4:50086656-50086675 None:intergenic 40.0%
TCACAGAAGTTCAGCAACAT+TGG - chr2.4:50088564-50088583 None:intergenic 40.0%
TCGAGCTTGAACTTAGAGTA+TGG + chr2.4:50087491-50087510 MS.gene025141:intron 40.0%
TGAGTCTGTAGATGCATTGA+AGG - chr2.4:50086125-50086144 None:intergenic 40.0%
TGATGTTCAGCAAGTGTCTT+TGG + chr2.4:50088370-50088389 MS.gene025141:CDS 40.0%
TGGAGATTATCTAGGTGTGA+AGG + chr2.4:50087005-50087024 MS.gene025141:CDS 40.0%
TTGCAAGAGAATCTTCTCAG+AGG + chr2.4:50086210-50086229 MS.gene025141:CDS 40.0%
! ACTCCGACTTGAATCTTAGA+TGG - chr2.4:50087975-50087994 None:intergenic 40.0%
! CAGTAGTAGGGTTTGTTTCA+AGG - chr2.4:50087610-50087629 None:intergenic 40.0%
! TCAACGTTGATAAGGAGGAA+TGG + chr2.4:50088114-50088133 MS.gene025141:CDS 40.0%
! TTTTGCTACCGACAGTAGTA+GGG - chr2.4:50087622-50087641 None:intergenic 40.0%
!! ATTCCTAGGTATGCTGTTTC+TGG + chr2.4:50087286-50087305 MS.gene025141:intron 40.0%
!! TTCCTAGGTATGCTGTTTCT+GGG + chr2.4:50087287-50087306 MS.gene025141:intron 40.0%
AAACACGGGAAGATCCAGAA+CGG - chr2.4:50088319-50088338 None:intergenic 45.0%
AAACTGCTTGATACCCTCCA+AGG - chr2.4:50088090-50088109 None:intergenic 45.0%
AATACCACGAGCCAGAAGAT+CGG - chr2.4:50088351-50088370 None:intergenic 45.0%
AGATAGTTCTCAGGCTGTGT+TGG - chr2.4:50088413-50088432 None:intergenic 45.0%
AGCATGTCAAATACACGTCC+AGG - chr2.4:50087111-50087130 None:intergenic 45.0%
AGCCATCAGCTATTCAGCAA+CGG + chr2.4:50086797-50086816 MS.gene025141:CDS 45.0%
AGCGGTGTTCATGTTGTTGT+TGG + chr2.4:50087081-50087100 MS.gene025141:CDS 45.0%
AGTGGAAGGTTTGGAAGGAA+AGG + chr2.4:50088449-50088468 MS.gene025141:CDS 45.0%
ATCATGCGTGAATTCCGTTC+TGG + chr2.4:50088302-50088321 MS.gene025141:CDS 45.0%
CATGTCAAATACACGTCCAG+GGG - chr2.4:50087109-50087128 None:intergenic 45.0%
CGTGCACTTGGAGATTATCT+AGG + chr2.4:50086997-50087016 MS.gene025141:CDS 45.0%
GAATCTTAGATGGCAACAGC+TGG - chr2.4:50087965-50087984 None:intergenic 45.0%
GATAGTTCTCAGGCTGTGTT+GGG - chr2.4:50088412-50088431 None:intergenic 45.0%
GATCAGCGCATTCTATCAAG+CGG + chr2.4:50087063-50087082 MS.gene025141:CDS 45.0%
GCATGTCAAATACACGTCCA+GGG - chr2.4:50087110-50087129 None:intergenic 45.0%
GTTATACAACAGGCTCAGTC+TGG + chr2.4:50086850-50086869 MS.gene025141:CDS 45.0%
TTCCACTACGACCAATACGA+TGG - chr2.4:50088436-50088455 None:intergenic 45.0%
TTCCCAGAAACAGCATACCT+AGG - chr2.4:50087292-50087311 None:intergenic 45.0%
! ATCGTATTGGTCGTAGTGGA+AGG + chr2.4:50088435-50088454 MS.gene025141:CDS 45.0%
! ATTGGTCGTAGTGGAAGGTT+TGG + chr2.4:50088440-50088459 MS.gene025141:CDS 45.0%
! GTTTTGCTACCGACAGTAGT+AGG - chr2.4:50087623-50087642 None:intergenic 45.0%
! TTTCTGGGAATTGCACCTCT+AGG + chr2.4:50087302-50087321 MS.gene025141:intron 45.0%
!! CTCAGAGGCATTTACGCATA+TGG + chr2.4:50086225-50086244 MS.gene025141:CDS 45.0%
AAGACTGCAACCTTCTGCTC+TGG + chr2.4:50086880-50086899 MS.gene025141:CDS 50.0%
ACACCAGACGAAAGGTTGAC+TGG + chr2.4:50088201-50088220 MS.gene025141:CDS 50.0%
AGACTGCAACCTTCTGCTCT+GGG + chr2.4:50086881-50086900 MS.gene025141:CDS 50.0%
CAAGACCAAGGCTTGGCATT+CGG - chr2.4:50086935-50086954 None:intergenic 50.0%
CACACTGTCTCAGCAACACA+CGG + chr2.4:50088251-50088270 MS.gene025141:CDS 50.0%
CAGACTCACAACTGATGCAC+AGG + chr2.4:50086137-50086156 MS.gene025141:intron 50.0%
CCATCAGCTATTCAGCAACG+GGG + chr2.4:50086799-50086818 MS.gene025141:CDS 50.0%
CGCTTCACAAGAATCCTCAC+AGG - chr2.4:50088056-50088075 None:intergenic 50.0%
CTGCAAAATCCCAGAGCAGA+AGG - chr2.4:50086893-50086912 None:intergenic 50.0%
CTTGTGATCTCAAGTGCCTC+AGG - chr2.4:50088017-50088036 None:intergenic 50.0%
GCATCAAACTGTGAACCCTC+TGG - chr2.4:50085946-50085965 None:intergenic 50.0%
GCCATCAGCTATTCAGCAAC+GGG + chr2.4:50086798-50086817 MS.gene025141:CDS 50.0%
GCTGATGAGATGCTTTCTCG+TGG + chr2.4:50087183-50087202 MS.gene025141:CDS 50.0%
GCTTGCTCAGCAGATTGAGA+AGG + chr2.4:50086969-50086988 MS.gene025141:CDS 50.0%
TTGCTGAGACAGTGTGGTCA+CGG - chr2.4:50088247-50088266 None:intergenic 50.0%
! AACAGGCTCAGTCTGGAACA+GGG + chr2.4:50086857-50086876 MS.gene025141:CDS 50.0%
! CATGTTGTTGTTGGTACCCC+TGG + chr2.4:50087090-50087109 MS.gene025141:CDS 50.0%
! CTCCATCGTATTGGTCGTAG+TGG + chr2.4:50088431-50088450 MS.gene025141:CDS 50.0%
! CTGAGGCACTTGAGATCACA+AGG + chr2.4:50088015-50088034 MS.gene025141:CDS 50.0%
! GTCGTAGTGGAAGGTTTGGA+AGG + chr2.4:50088444-50088463 MS.gene025141:CDS 50.0%
! GTGAAGGTGCATGCTTGTGT+CGG + chr2.4:50087021-50087040 MS.gene025141:CDS 50.0%
AAGGTGCATGCTTGTGTCGG+AGG + chr2.4:50087024-50087043 MS.gene025141:CDS 55.0%
ACGAGTAGGTGCCAAGACCA+AGG - chr2.4:50086947-50086966 None:intergenic 55.0%
CAGCCAGTCAACCTTTCGTC+TGG - chr2.4:50088207-50088226 None:intergenic 55.0%
CCCCGTTGCTGAATAGCTGA+TGG - chr2.4:50086802-50086821 None:intergenic 55.0%
CGAATGCCAAGCCTTGGTCT+TGG + chr2.4:50086933-50086952 MS.gene025141:CDS 55.0%
CGTGATGAGCTCACCTTGGA+GGG + chr2.4:50088074-50088093 MS.gene025141:CDS 55.0%
CTCAGCAACACACGGAGACA+TGG + chr2.4:50088259-50088278 MS.gene025141:CDS 55.0%
CTCTGCTACAATGCCTCCTG+AGG + chr2.4:50087998-50088017 MS.gene025141:CDS 55.0%
GAAGCGTGATGAGCTCACCT+TGG + chr2.4:50088070-50088089 MS.gene025141:CDS 55.0%
GAGAAGGTCATGCGTGCACT+TGG + chr2.4:50086985-50087004 MS.gene025141:CDS 55.0%
GTGATCTCAAGTGCCTCAGG+AGG - chr2.4:50088014-50088033 None:intergenic 55.0%
TAGGTGCCAAGACCAAGGCT+TGG - chr2.4:50086942-50086961 None:intergenic 55.0%
TGCTGAGCAAGCTCACGAGT+AGG - chr2.4:50086961-50086980 None:intergenic 55.0%
TGTGACCGAATGCCAAGCCT+TGG + chr2.4:50086927-50086946 MS.gene025141:CDS 55.0%
! CAACAGGCTCAGTCTGGAAC+AGG + chr2.4:50086856-50086875 MS.gene025141:CDS 55.0%
! CGTGTGTTGCTGAGACAGTG+TGG - chr2.4:50088253-50088272 None:intergenic 55.0%
! GCGCTGATCTTCACGAACAC+TGG - chr2.4:50087052-50087071 None:intergenic 55.0%
!! ACTACCGATCTTCTGGCTCG+TGG + chr2.4:50088344-50088363 MS.gene025141:CDS 55.0%
GCGTGATGAGCTCACCTTGG+AGG + chr2.4:50088073-50088092 MS.gene025141:CDS 60.0%
! ATGGCAGGCGTTGCACCAGA+GGG + chr2.4:50085928-50085947 MS.gene025141:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 50085928 50088583 50085928 ID=MS.gene025141
chr2.4 mRNA 50085928 50088583 50085928 ID=MS.gene025141.t1;Parent=MS.gene025141
chr2.4 exon 50085928 50085995 50085928 ID=MS.gene025141.t1.exon1;Parent=MS.gene025141.t1
chr2.4 CDS 50085928 50085995 50085928 ID=cds.MS.gene025141.t1;Parent=MS.gene025141.t1
chr2.4 exon 50086140 50086246 50086140 ID=MS.gene025141.t1.exon2;Parent=MS.gene025141.t1
chr2.4 CDS 50086140 50086246 50086140 ID=cds.MS.gene025141.t1;Parent=MS.gene025141.t1
chr2.4 exon 50086788 50087218 50086788 ID=MS.gene025141.t1.exon3;Parent=MS.gene025141.t1
chr2.4 CDS 50086788 50087218 50086788 ID=cds.MS.gene025141.t1;Parent=MS.gene025141.t1
chr2.4 exon 50087948 50088583 50087948 ID=MS.gene025141.t1.exon4;Parent=MS.gene025141.t1
chr2.4 CDS 50087948 50088583 50087948 ID=cds.MS.gene025141.t1;Parent=MS.gene025141.t1
Gene Sequence

>MS.gene025141

ATGGCAGGCGTTGCACCAGAGGGTTCACAGTTTGATGCTAAGCAGTTTGATTCAAAAATGAATGAGTTGTAAGTACAACCCCGTCATAGCTTTGTTTTTATTTTTATTTTTTATTTTTTTAAGTGAAGTATACAATTCTGTTTTTTTAGCGTTTAGTGTTACTATTCTTGTTTTTGTCTAACTTCGGGTACATACCTTCAATGCATCTACAGACTCACAACTGATGCACAGGACTTCTTCACATCTTATGATGAGGTCTATGATAGTTTTGATGCTATGGGCTTGCAAGAGAATCTTCTCAGAGGCATTTACGCATATGGTAGATTTAATCTTTTCTGTTCTTTTCTACTTCCTTCTGTACCAATCTTTAATTTTTTTAACCAGATGGGATTTTTTTCGTGCTTTCATCTTCTAAGAATTAAAATGTTATTGTTGGGCTCTGTTGGTCCTTAAATTTTGTATTGTATCTGGCAGCTAAAAACCTTCCATACTGTGAAATATACCCGTTTTGTTTTGCTTCCTATTTATCACGGTTGTTTCTTTATAATTTTTGAAATTGGAATATTTTTATCACTACCATAAGTTCGATTTCTACTTTTTCATAAAAAAAACCCAAGATTGTTCTTGTTTCTGTATGAGTTTTATGCATGTAAAATGAATCTATCATCCTTTTTCTACGTAGTATATCCTCTCATTTACTATAAAAACTTTTTGCATATTTTATTCCACCTTGCTAATGTTGTATGACTTTATTCAGCTGTATTTTTGTACAAACAAGTATTTGCACTGCCGAGTCTTTTATTGATACATAAATAAATGTACACATAAATCCTTAATAACTAGTTTGATTTGTGTTTTAGGTTTTGAGAAGCCATCAGCTATTCAGCAACGGGGAATAGTTCCATTTATCAAGGGTCTTGATGTTATACAACAGGCTCAGTCTGGAACAGGGAAGACTGCAACCTTCTGCTCTGGGATTTTGCAGCAACTTGACTACAGTGTGACCGAATGCCAAGCCTTGGTCTTGGCACCTACTCGTGAGCTTGCTCAGCAGATTGAGAAGGTCATGCGTGCACTTGGAGATTATCTAGGTGTGAAGGTGCATGCTTGTGTCGGAGGAACCAGTGTTCGTGAAGATCAGCGCATTCTATCAAGCGGTGTTCATGTTGTTGTTGGTACCCCTGGACGTGTATTTGACATGCTGCGCAGACAGTCGCTTCGACCAGATTACATCAAAATGTTCGTATTGGACGAGGCTGATGAGATGCTTTCTCGTGGTTTTAAAGATCAGGTACCTTCTAATTTTTGTACACTTTCTAAAATTTCTTAATGGAAAATATTAAGGTACTAGTAATCACATTCCTAGGTATGCTGTTTCTGGGAATTGCACCTCTAGGAACATTGTTCTTATCTATTCATAATTCTAGTAATGTTTTCAAAATTTATTGTTAAAAAACAAATTAAGGTGGGAAATTGTGTAGTAAGGATAAAAGTGGAAAATTATAGTTTGATGTGAGTTTGAAAATAACTGCTCTCTCCATAGAAATTTAAGATTCCCATTTCTCGAGCTTGAACTTAGAGTATGGATATATTACAATCATGTAAATATTCTAATGCCAGAGAATAATTTTTTAACCTGTTTCAAAATGAAATATATTTTTTGTGAATGATTTTTCATACCTTGAAACAAACCCTACTACTGTCGGTAGCAAAACTAAAATGTGGAATTAAGTTCCTTTCAATCTTGAAGATTTTGATCTTTATTTTACAAACCGAGGATTAATTTTTTAGAAATTGTTTGAAACATGGAAATATACATTCTGAAGCTCACTATCCCAGTAAATAGTAATATTATTGGATGCCCTTAAACACAGTCTTGTACTATTAGGATTAGGAGCACAACTAAAACTTGAAAAAAGTTCCTCTCAGCTATCTTGAAGATTTGATTTTTATTTGCCTACTGATTTATTGTCCTAAAAGAATTAATGTATGCATGTTATAACCTTCATTTTATTTTCAGATCTATGATATATTCCAGCTGTTGCCATCTAAGATTCAAGTCGGAGTTTTCTCTGCTACAATGCCTCCTGAGGCACTTGAGATCACAAGGAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAGCTCACCTTGGAGGGTATCAAGCAGTTTTATGTCAACGTTGATAAGGAGGAATGGAAGCTCGACACACTCTGTGATCTTTATGAGACTTTGGCTATCACCCAGAGTGTCATTTTTGTTAACACCAGACGAAAGGTTGACTGGCTGACTGACAAGATGAGAAGCCGTGACCACACTGTCTCAGCAACACACGGAGACATGGACCAAAATACTAGGGACATTATCATGCGTGAATTCCGTTCTGGATCTTCCCGTGTTTTGATAACTACCGATCTTCTGGCTCGTGGTATTGATGTTCAGCAAGTGTCTTTGGTTATAAATTATGATCTCCCAACACAGCCTGAGAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGAAGGAAAGGTGTTGCTATTAACTTCGTCACAACTGATGACGAAAGAATGCTGAGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTGCCTTCCAATGTTGCTGAACTTCTGTGA
Protein sequence

>MS.gene025141.t1

MAGVAPEGSQFDAKQFDSKMNELLTTDAQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYSVTECQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILSSGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPSKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLDTLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTTDDERMLSDIQKFYNVLIEELPSNVAELL