AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60217

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60217.t1 MTR_4g010565 100.000 344 0 0 1 344 161 504 0.0 712
MS.gene60217.t1 MTR_1g012420 93.314 344 23 0 1 344 84 427 0.0 666
MS.gene60217.t1 MTR_3g107420 92.151 344 27 0 1 344 84 427 0.0 663
MS.gene60217.t1 MTR_3g107420 93.865 163 10 0 1 163 84 246 2.66e-110 324
MS.gene60217.t1 MTR_2g039960 39.466 337 198 6 1 336 78 409 1.04e-70 226
MS.gene60217.t1 MTR_4g125970 39.169 337 199 6 1 336 78 409 1.31e-69 223
MS.gene60217.t1 MTR_2g099620 39.466 337 198 6 1 336 62 393 1.59e-69 223
MS.gene60217.t1 MTR_2g099620 39.466 337 198 6 1 336 78 409 1.81e-69 223
MS.gene60217.t1 MTR_4g125970 39.169 337 199 6 1 336 95 426 3.14e-69 223
MS.gene60217.t1 MTR_8g018400 38.872 337 200 6 1 336 78 409 2.27e-68 220
MS.gene60217.t1 MTR_7g076820 37.243 341 206 6 2 341 188 521 5.59e-68 222
MS.gene60217.t1 MTR_7g076800 37.243 341 206 6 2 341 179 512 1.61e-67 221
MS.gene60217.t1 MTR_7g076830 37.243 341 206 6 2 341 181 514 1.96e-67 220
MS.gene60217.t1 MTR_2g438110 36.527 334 204 7 2 333 72 399 4.13e-64 209
MS.gene60217.t1 MTR_3g467550 33.851 322 194 6 2 308 124 441 3.19e-53 183
MS.gene60217.t1 MTR_3g467550 33.851 322 194 6 2 308 155 472 4.81e-53 183
MS.gene60217.t1 MTR_2g100230 34.169 319 197 7 2 312 134 447 9.90e-52 179
MS.gene60217.t1 MTR_7g056830 33.138 341 207 8 2 334 48 375 1.72e-51 177
MS.gene60217.t1 MTR_6g082080 33.138 341 207 8 2 334 48 375 1.89e-51 176
MS.gene60217.t1 MTR_4g134170 34.513 339 183 6 2 310 325 654 3.57e-50 178
MS.gene60217.t1 MTR_3g083690 34.262 359 205 9 2 336 183 534 1.56e-49 175
MS.gene60217.t1 MTR_1g054630 35.347 331 191 7 2 312 192 519 3.30e-48 171
MS.gene60217.t1 MTR_6g005320 34.218 339 182 7 2 310 287 614 4.86e-48 172
MS.gene60217.t1 MTR_4g114120 34.835 333 191 7 2 311 178 507 3.26e-47 169
MS.gene60217.t1 MTR_4g049910 31.519 349 214 8 2 335 96 434 4.48e-46 162
MS.gene60217.t1 MTR_4g105100 30.094 319 210 6 2 312 240 553 3.65e-45 163
MS.gene60217.t1 MTR_3g449250 31.232 349 209 10 2 334 128 461 1.35e-44 160
MS.gene60217.t1 MTR_1g095810 32.000 325 198 8 2 310 183 500 1.78e-44 161
MS.gene60217.t1 MTR_3g449250 31.232 349 209 10 2 334 128 461 2.23e-44 160
MS.gene60217.t1 MTR_2g066050 31.420 331 214 9 3 326 92 416 6.67e-44 158
MS.gene60217.t1 MTR_5g090120 31.445 353 216 10 2 340 119 459 1.24e-43 159
MS.gene60217.t1 MTR_2g041140 32.219 329 214 6 2 324 179 504 4.58e-43 159
MS.gene60217.t1 MTR_3g467550 32.667 300 177 7 2 283 155 447 1.23e-42 154
MS.gene60217.t1 MTR_2g032630 32.000 325 204 8 2 314 534 853 1.30e-42 158
MS.gene60217.t1 MTR_3g049990 32.219 329 213 7 2 324 190 514 3.87e-42 156
MS.gene60217.t1 MTR_6g005550 31.529 314 203 8 2 308 203 511 3.97e-42 155
MS.gene60217.t1 MTR_1g061970 29.268 369 212 9 2 335 121 475 1.27e-41 151
MS.gene60217.t1 MTR_1g026830 30.259 347 211 10 2 332 185 516 4.50e-41 151
MS.gene60217.t1 MTR_0915s0010 34.351 262 165 5 2 262 27 282 7.95e-41 144
MS.gene60217.t1 MTR_7g109720 31.776 321 199 10 2 310 270 582 2.22e-40 151
MS.gene60217.t1 MTR_8g018370 30.631 333 204 7 2 333 11 317 2.55e-40 144
MS.gene60217.t1 MTR_3g071620 29.118 340 216 5 2 317 54 392 3.89e-40 149
MS.gene60217.t1 MTR_7g100820 33.010 309 194 8 7 310 177 477 5.78e-40 149
MS.gene60217.t1 MTR_2g007000 31.875 320 201 8 2 310 542 855 2.20e-39 149
MS.gene60217.t1 MTR_8g027780 31.402 328 203 9 2 310 144 468 5.54e-39 145
MS.gene60217.t1 MTR_8g027775 30.793 328 205 9 2 310 140 464 1.84e-38 144
MS.gene60217.t1 MTR_4g079320 31.447 318 205 11 1 311 56 367 9.77e-38 143
MS.gene60217.t1 MTR_8g027770 29.730 370 230 9 2 344 239 605 2.32e-37 142
MS.gene60217.t1 MTR_5g027840 31.288 326 199 7 2 310 140 457 9.29e-36 136
MS.gene60217.t1 MTR_8g097000 30.000 350 216 7 2 333 326 664 8.80e-35 135
MS.gene60217.t1 MTR_2g055850 29.245 318 211 8 2 309 129 442 7.60e-34 131
MS.gene60217.t1 MTR_4g112340 29.814 322 205 11 2 309 135 449 7.42e-33 129
MS.gene60217.t1 MTR_6g033070 29.630 351 219 11 7 341 183 521 1.78e-32 127
MS.gene60217.t1 MTR_7g057250 26.570 414 227 12 2 343 202 610 4.80e-32 127
MS.gene60217.t1 MTR_6g056080 30.312 320 201 11 2 306 141 453 5.71e-32 126
MS.gene60217.t1 MTR_5g092560 27.692 325 207 10 2 309 114 427 9.15e-32 126
MS.gene60217.t1 MTR_1g046570 27.614 373 229 10 1 337 28 395 1.03e-31 123
MS.gene60217.t1 MTR_2g063080 26.615 387 221 8 2 329 147 529 1.40e-31 125
MS.gene60217.t1 MTR_8g027780 31.081 296 182 9 2 278 144 436 3.54e-30 120
MS.gene60217.t1 MTR_1g102410 28.274 336 203 10 2 306 82 410 5.47e-30 120
MS.gene60217.t1 MTR_4g059460 27.621 391 190 16 3 309 84 465 7.56e-29 117
MS.gene60217.t1 MTR_2g043360 26.506 415 210 18 3 333 81 484 4.34e-28 115
MS.gene60217.t1 MTR_4g105100 27.174 276 188 6 2 269 240 510 6.11e-28 114
MS.gene60217.t1 MTR_4g068990 27.744 328 202 13 2 309 114 426 2.38e-27 113
MS.gene60217.t1 MTR_4g112320 32.189 233 145 7 85 309 40 267 2.85e-27 111
MS.gene60217.t1 MTR_5g069330 25.846 325 229 7 2 320 42 360 1.10e-26 109
MS.gene60217.t1 MTR_5g069330 25.846 325 229 7 2 320 46 364 1.25e-26 109
MS.gene60217.t1 MTR_6g039840 26.190 336 209 12 3 310 149 473 7.57e-25 105
MS.gene60217.t1 MTR_2g043360 27.333 300 142 11 86 312 51 347 1.46e-23 101
MS.gene60217.t1 MTR_3g118120 28.324 346 196 10 8 313 176 509 4.54e-23 100
MS.gene60217.t1 MTR_6g039840 26.578 301 191 10 29 310 8 297 6.80e-23 99.8
MS.gene60217.t1 MTR_5g092560 28.293 205 138 5 111 309 15 216 1.99e-21 95.5
MS.gene60217.t1 MTR_1g022305 25.962 312 164 10 2 310 42 289 3.86e-21 94.4
MS.gene60217.t1 MTR_4g068990 29.187 209 130 7 111 309 15 215 8.81e-20 90.5
MS.gene60217.t1 MTR_0427s0010 29.048 210 111 6 141 335 23 209 2.07e-19 86.3
MS.gene60217.t1 MTR_4g131750 23.720 371 231 9 2 332 120 478 1.04e-17 84.3
MS.gene60217.t1 MTR_4g077370 32.911 158 100 5 3 157 289 443 1.78e-16 80.5
MS.gene60217.t1 MTR_8g007360 72.000 50 12 1 46 95 10 57 1.67e-15 70.9
MS.gene60217.t1 MTR_5g077690 24.138 377 212 12 2 310 71 441 1.74e-15 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60217.t1 AT5G11170 92.733 344 25 0 1 344 1 344 0.0 666
MS.gene60217.t1 AT5G11170 92.733 344 25 0 1 344 84 427 0.0 664
MS.gene60217.t1 AT5G11200 92.733 344 25 0 1 344 84 427 0.0 664
MS.gene60217.t1 AT5G11200 82.857 385 25 1 1 344 84 468 0.0 644
MS.gene60217.t1 AT5G11200 70.968 403 58 4 1 344 84 486 0.0 553
MS.gene60217.t1 AT3G61240 38.123 341 203 6 2 341 163 496 9.16e-72 231
MS.gene60217.t1 AT3G61240 38.123 341 203 6 2 341 163 496 9.16e-72 231
MS.gene60217.t1 AT2G45810 38.123 341 203 6 2 341 193 526 1.90e-71 231
MS.gene60217.t1 AT3G13920 39.169 337 199 6 1 336 67 398 4.34e-70 224
MS.gene60217.t1 AT3G13920 39.169 337 199 6 1 336 72 403 4.65e-70 224
MS.gene60217.t1 AT3G13920 39.169 337 199 6 1 336 77 408 5.80e-70 224
MS.gene60217.t1 AT1G72730 38.872 337 200 6 1 336 79 410 6.11e-70 224
MS.gene60217.t1 AT4G00660 38.416 341 202 6 2 341 170 503 7.84e-70 226
MS.gene60217.t1 AT4G00660 38.416 341 202 6 2 341 170 503 7.84e-70 226
MS.gene60217.t1 AT1G54270 38.872 337 200 6 1 336 72 403 4.68e-68 219
MS.gene60217.t1 AT1G54270 38.872 337 200 6 1 336 77 408 5.69e-68 219
MS.gene60217.t1 AT3G13920 40.193 311 181 5 1 310 77 383 1.45e-66 215
MS.gene60217.t1 AT3G13920 40.193 311 181 5 1 310 81 387 2.12e-66 215
MS.gene60217.t1 AT3G19760 37.425 334 201 7 2 333 74 401 6.97e-66 213
MS.gene60217.t1 AT1G51380 37.126 334 202 7 2 333 61 388 1.90e-63 207
MS.gene60217.t1 AT3G53110 32.951 349 208 9 3 332 134 475 6.10e-55 187
MS.gene60217.t1 AT1G55150 35.093 322 190 9 2 312 138 451 2.70e-50 175
MS.gene60217.t1 AT1G55150 35.093 322 190 9 2 312 163 476 4.51e-50 175
MS.gene60217.t1 AT5G26742 32.298 322 202 5 2 312 48 364 2.97e-49 175
MS.gene60217.t1 AT5G26742 32.298 322 202 5 2 312 141 457 7.82e-49 175
MS.gene60217.t1 AT5G26742 32.298 322 202 5 2 312 141 457 7.88e-49 175
MS.gene60217.t1 AT1G28180 34.169 319 193 7 2 310 12 323 4.50e-48 166
MS.gene60217.t1 AT5G60990 31.624 351 209 9 2 334 48 385 1.33e-47 167
MS.gene60217.t1 AT5G63120 31.861 317 203 9 2 310 204 515 2.78e-47 169
MS.gene60217.t1 AT2G33730 33.041 342 199 7 2 318 352 688 5.46e-47 169
MS.gene60217.t1 AT3G58510 32.958 355 212 11 2 336 190 538 6.42e-46 165
MS.gene60217.t1 AT3G58510 32.958 355 212 11 2 336 190 538 6.42e-46 165
MS.gene60217.t1 AT3G58510 32.958 355 212 11 2 336 190 538 6.42e-46 165
MS.gene60217.t1 AT3G58570 35.780 327 187 8 2 308 185 508 1.77e-45 164
MS.gene60217.t1 AT1G16280 32.110 327 211 8 2 322 97 418 2.61e-45 161
MS.gene60217.t1 AT4G16630 31.503 346 217 10 2 335 206 543 6.06e-45 164
MS.gene60217.t1 AT3G22310 30.882 340 222 6 2 334 155 488 7.10e-44 159
MS.gene60217.t1 AT3G22310 30.882 340 222 6 2 334 155 488 8.38e-44 159
MS.gene60217.t1 AT2G42520 32.584 356 214 10 2 336 198 548 1.49e-43 159
MS.gene60217.t1 AT2G42520 32.584 356 214 10 2 336 198 548 1.49e-43 159
MS.gene60217.t1 AT2G42520 32.584 356 214 10 2 336 198 548 1.49e-43 159
MS.gene60217.t1 AT3G22330 30.725 345 214 9 2 333 143 475 1.54e-43 159
MS.gene60217.t1 AT3G01540 31.195 343 221 11 2 336 197 532 4.52e-43 157
MS.gene60217.t1 AT3G01540 31.195 343 221 11 2 336 197 532 4.52e-43 157
MS.gene60217.t1 AT3G01540 31.195 343 221 11 2 336 197 532 4.52e-43 157
MS.gene60217.t1 AT3G01540 31.195 343 221 11 2 336 197 532 4.52e-43 157
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 69 377 1.03e-42 155
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 69 377 1.03e-42 155
MS.gene60217.t1 AT5G51280 32.110 327 195 9 2 310 185 502 1.67e-42 155
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 200 508 2.91e-42 155
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 200 508 2.97e-42 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 230 543 3.24e-42 156
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 208 516 3.34e-42 155
MS.gene60217.t1 AT5G14610 32.166 314 201 9 2 308 268 576 5.24e-42 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT1G20920 31.562 320 202 9 2 310 568 881 1.24e-41 155
MS.gene60217.t1 AT4G33370 32.615 325 196 8 2 310 136 453 1.95e-41 152
MS.gene60217.t1 AT2G47330 32.710 321 196 10 2 310 267 579 2.34e-41 154
MS.gene60217.t1 AT1G71370 30.000 320 211 5 2 309 55 373 3.18e-41 151
MS.gene60217.t1 AT3G06480 31.962 316 203 7 2 310 474 784 6.91e-41 153
MS.gene60217.t1 AT1G31970 32.919 322 200 7 2 310 153 471 1.18e-40 150
MS.gene60217.t1 AT5G63630 29.358 327 211 6 2 310 93 417 3.93e-38 142
MS.gene60217.t1 AT5G05450 27.964 329 215 5 2 309 55 382 1.52e-37 142
MS.gene60217.t1 AT5G63630 29.573 328 209 7 2 310 287 611 3.48e-37 142
MS.gene60217.t1 AT1G77030 30.380 316 209 10 1 310 66 376 1.29e-36 140
MS.gene60217.t1 AT3G18600 31.013 316 200 12 2 306 128 436 3.96e-36 137
MS.gene60217.t1 AT5G08610 28.571 350 221 7 2 333 421 759 1.23e-34 134
MS.gene60217.t1 AT5G08620 29.052 327 212 6 2 310 119 443 1.34e-34 133
MS.gene60217.t1 AT5G62190 30.168 358 226 11 2 342 135 485 1.62e-34 134
MS.gene60217.t1 AT3G09720 31.410 312 195 9 7 310 185 485 1.77e-34 133
MS.gene60217.t1 AT3G09720 31.410 312 195 9 7 310 185 485 1.77e-34 133
MS.gene60217.t1 AT3G09620 31.348 319 175 10 2 310 435 719 2.62e-34 134
MS.gene60217.t1 AT3G09620 31.348 319 175 10 2 310 436 720 2.64e-34 134
MS.gene60217.t1 AT3G02065 30.154 325 201 9 2 310 12 326 8.17e-34 128
MS.gene60217.t1 AT3G02065 30.247 324 200 9 3 310 150 463 1.67e-32 127
MS.gene60217.t1 AT3G02065 30.247 324 200 9 3 310 150 463 1.67e-32 127
MS.gene60217.t1 AT3G16840 28.744 414 217 10 2 343 231 638 4.51e-32 127
MS.gene60217.t1 AT3G16840 28.744 414 217 10 2 343 230 637 5.35e-32 127
MS.gene60217.t1 AT1G12770 25.691 362 212 6 2 307 149 509 8.89e-32 125
MS.gene60217.t1 AT1G12770 25.691 362 212 6 2 307 149 509 8.89e-32 125
MS.gene60217.t1 AT1G63250 29.129 333 205 10 2 310 367 692 1.52e-31 125
MS.gene60217.t1 AT5G65900 29.412 323 197 12 2 306 193 502 3.50e-31 124
MS.gene60217.t1 AT1G51380 36.744 215 129 6 2 214 61 270 5.11e-31 119
MS.gene60217.t1 AT5G63120 29.787 282 183 10 2 275 204 478 2.54e-30 120
MS.gene60217.t1 AT5G63120 29.787 282 183 10 2 275 204 478 2.54e-30 120
MS.gene60217.t1 AT5G63120 29.787 282 183 10 2 275 204 478 2.54e-30 120
MS.gene60217.t1 AT5G63120 29.710 276 181 9 2 269 204 474 2.86e-30 120
MS.gene60217.t1 AT2G07750 28.228 333 208 10 2 310 414 739 5.88e-29 118
MS.gene60217.t1 AT5G54910 28.963 328 204 13 2 312 110 425 4.08e-28 115
MS.gene60217.t1 AT5G14610 29.965 287 187 10 2 281 208 487 2.65e-27 112
MS.gene60217.t1 AT4G09730 27.679 336 206 11 2 310 151 476 4.18e-27 112
MS.gene60217.t1 AT2G40700 26.992 389 199 11 2 309 70 454 6.65e-27 112
MS.gene60217.t1 AT3G06480 29.329 283 190 6 2 278 474 752 1.44e-26 111
MS.gene60217.t1 AT1G51380 36.170 188 114 5 2 187 61 244 4.74e-24 100
MS.gene60217.t1 AT1G51380 36.170 188 114 5 2 187 61 244 5.02e-24 100
MS.gene60217.t1 AT4G15850 27.005 374 206 11 2 311 74 444 2.73e-23 100
MS.gene60217.t1 AT5G19210 25.994 327 205 9 2 298 115 434 5.95e-22 96.7
MS.gene60217.t1 AT1G71280 27.817 284 160 12 2 271 56 308 2.32e-19 87.8
MS.gene60217.t1 AT3G06980 25.364 343 209 9 4 309 415 747 3.74e-19 89.4
MS.gene60217.t1 AT1G71280 27.817 284 160 12 2 271 56 308 6.86e-19 87.8
MS.gene60217.t1 AT4G34910 24.355 349 208 9 2 298 85 429 1.47e-16 81.3
MS.gene60217.t1 AT5G19210 24.231 260 168 6 63 298 23 277 4.13e-14 72.4
MS.gene60217.t1 AT5G19210 24.231 260 168 6 63 298 23 277 4.13e-14 72.4

Find 72 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTCTTCATGGTCTTGTTC+AGG 0.261990 4.3:-86047612 MS.gene60217:intron
CCATCAATTTGCATACATTC+TGG 0.285699 4.3:-86047361 MS.gene60217:CDS
GTCATTTGCCAAGCAAAATC+TGG 0.314855 4.3:-86049073 MS.gene60217:CDS
CACAAGAGAACTAGCTTATC+AGG 0.318557 4.3:-86048963 MS.gene60217:intron
AAGACCATGAAGAGTCAATT+TGG 0.351581 4.3:+86047619 None:intergenic
GTGGGTAGAGCTGGTAGATT+TGG 0.365949 4.3:-86045692 MS.gene60217:CDS
CACATTGTTGTTGGAACACC+TGG 0.373009 4.3:-86048671 MS.gene60217:CDS
CCAGAATGTATGCAAATTGA+TGG 0.376590 4.3:+86047361 None:intergenic
TGACTCTGAAGTTCTTAATC+AGG 0.377300 4.3:-86045630 MS.gene60217:intron
GTGGAGGTGTCAATTTGTTC+AGG 0.377618 4.3:+86045459 None:intergenic
CAGCAAATTGATCCTGTTCC+TGG 0.384946 4.3:-86049013 MS.gene60217:CDS
CAAGGTCCTTATCTCTAGTC+AGG 0.399693 4.3:+86048636 None:intergenic
ACATCATCACTTGTTTATCA+TGG 0.400130 4.3:+86048415 None:intergenic
TAGGTTTGAGGTTGATATAA+AGG 0.405007 4.3:-86045487 MS.gene60217:CDS
GAGGCCAAATTGACTCTTCA+TGG 0.406230 4.3:-86047623 MS.gene60217:CDS
TGCATGAATTTCTTGCAAAC+AGG 0.412830 4.3:+86048363 None:intergenic
AATTGATCCTGTTCCTGGTC+AGG 0.417735 4.3:-86049008 MS.gene60217:CDS
GTCACAAAACTTGATCAATT+AGG 0.428105 4.3:+86045140 None:intergenic
AACTGGTAGGTGGGTAGAGC+TGG 0.430229 4.3:-86045701 MS.gene60217:intron
TTCAGCTTACGATTCTTCTC+TGG 0.442230 4.3:+86047484 None:intergenic
CATCATCACTTGTTTATCAT+GGG 0.463946 4.3:+86048416 None:intergenic
ATCATCTACATAAATTTCCA+TGG 0.469432 4.3:+86047646 None:intergenic
AGATAGGTATCAGCAGAATC+AGG 0.470818 4.3:+86045866 None:intergenic
TCAGGTGCAATCTAGGTTTG+AGG 0.476362 4.3:-86045499 MS.gene60217:intron
GCTGCAACCTGACCAGGAAC+AGG 0.478885 4.3:+86049001 None:intergenic
TGCTGAACCTCTCAACTTCG+TGG 0.490700 4.3:+86048780 None:intergenic
TTTCAGATTGAAGCGATACA+AGG 0.493825 4.3:-86045987 MS.gene60217:intron
CTGCTGATACCTATCTTCAC+AGG 0.495663 4.3:-86045859 MS.gene60217:CDS
AGCACTTACCCTGTGAAGAT+AGG 0.504586 4.3:+86045850 None:intergenic
GAGTGCCCACACATTGTTGT+TGG 0.508601 4.3:-86048680 MS.gene60217:CDS
CTGGAATGTCACAAGAAGAA+AGG 0.515744 4.3:-86047342 MS.gene60217:intron
CTATGGTGGTGTCAACATCA+AGG 0.524822 4.3:-86048726 MS.gene60217:CDS
GCTGGTAGATTTGGCACCAA+GGG 0.527211 4.3:-86045683 MS.gene60217:CDS
ACAAGTGCTGCAACCTGACC+AGG 0.529516 4.3:+86048995 None:intergenic
TGATGAATGTGACAAGATGC+TGG 0.533632 4.3:-86048585 MS.gene60217:CDS
TTGCCAAGCAAAATCTGGGA+TGG 0.544090 4.3:-86049068 MS.gene60217:CDS
CTGACTAGAGATAAGGACCT+TGG 0.544589 4.3:-86048635 MS.gene60217:CDS
TCATTTGCCAAGCAAAATCT+GGG 0.556430 4.3:-86049072 MS.gene60217:CDS
AGCTGGTAGATTTGGCACCA+AGG 0.557173 4.3:-86045684 MS.gene60217:CDS
CTCGTGGCAACCGATTTAGT+AGG 0.561671 4.3:-86045938 MS.gene60217:CDS
CAGAATGTATGCAAATTGAT+GGG 0.563982 4.3:+86047362 None:intergenic
TCTTGCCCTGACTAGAGATA+AGG 0.564398 4.3:-86048642 MS.gene60217:CDS
TGCCAAGCAAAATCTGGGAT+GGG 0.564980 4.3:-86049067 MS.gene60217:CDS
GGCAACCGATTTAGTAGGGA+GGG 0.566914 4.3:-86045933 MS.gene60217:CDS
GACAAGATGCTGGAATCACT+TGG 0.566919 4.3:-86048575 MS.gene60217:intron
AAAAGTAATTGCAAGCCCCT+TGG 0.567368 4.3:+86045667 None:intergenic
CGATACAAGGGCTTCAAAGA+GGG 0.568834 4.3:-86045974 MS.gene60217:CDS
TCGTGGCAACCGATTTAGTA+GGG 0.570668 4.3:-86045937 MS.gene60217:CDS
GGTGTTCCAACAACAATGTG+TGG 0.577454 4.3:+86048674 None:intergenic
CAACCGATTTAGTAGGGAGG+GGG 0.579596 4.3:-86045931 MS.gene60217:CDS
TCATCTACATAAATTTCCAT+GGG 0.581419 4.3:+86047647 None:intergenic
GCAACCGATTTAGTAGGGAG+GGG 0.585593 4.3:-86045932 MS.gene60217:CDS
AGATTTGCCACGAAGTTGAG+AGG 0.588345 4.3:-86048787 MS.gene60217:CDS
GGAAATTTATGTAGATGATG+AGG 0.602038 4.3:-86047642 MS.gene60217:CDS
GCGATACAAGGGCTTCAAAG+AGG 0.604199 4.3:-86045975 MS.gene60217:CDS
AAGGTCCTTATCTCTAGTCA+GGG 0.605894 4.3:+86048637 None:intergenic
ACCTTGGTTTGAAGAATGTG+AGG 0.606908 4.3:-86048619 MS.gene60217:CDS
TGAATTTCTTGCAAACAGGT+CGG 0.607834 4.3:+86048367 None:intergenic
GTCAGGGCAAGAATCCTCCC+AGG 0.611803 4.3:+86048653 None:intergenic
ATCATCACTTGTTTATCATG+GGG 0.613006 4.3:+86048417 None:intergenic
GATACAAGGGCTTCAAAGAG+GGG 0.617527 4.3:-86045973 MS.gene60217:CDS
GTGTTCCAACAACAATGTGT+GGG 0.623994 4.3:+86048675 None:intergenic
GCCTCACATTCTTCAAACCA+AGG 0.633068 4.3:+86048618 None:intergenic
TTCAGATTGAAGCGATACAA+GGG 0.636996 4.3:-86045986 MS.gene60217:intron
AAGTATCTTACTGTATGTGG+AGG 0.637443 4.3:+86045443 None:intergenic
GATAAGCTAGTTCTCTTGTG+TGG 0.648872 4.3:+86048966 None:intergenic
CTGGTAGATTTGGCACCAAG+GGG 0.659086 4.3:-86045682 MS.gene60217:CDS
TGCTGATACCTATCTTCACA+GGG 0.659100 4.3:-86045858 MS.gene60217:intron
ACATTGTTGTTGGAACACCT+GGG 0.674278 4.3:-86048670 MS.gene60217:CDS
GGGGAAGCAGAGAATTCTCG+TGG 0.676806 4.3:-86045954 MS.gene60217:CDS
TGGCAACCGATTTAGTAGGG+AGG 0.690164 4.3:-86045934 MS.gene60217:CDS
TTGTTGTTGGAACACCTGGG+AGG 0.722510 4.3:-86048667 MS.gene60217:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACTAATAGATTTTTTTT+TGG - chr4.3:86048115-86048134 MS.gene60217:intron 10.0%
!! AAATGCATTTAAACAAAATA+TGG + chr4.3:86046256-86046275 None:intergenic 15.0%
!! ACAAAATTAAATGAATATGA+GGG + chr4.3:86046026-86046045 None:intergenic 15.0%
!! AGATTCTTTCTATTAATTTA+AGG - chr4.3:86048089-86048108 MS.gene60217:intron 15.0%
!! GATTCTTTCTATTAATTTAA+GGG - chr4.3:86048090-86048109 MS.gene60217:intron 15.0%
!! TAAGCAAAAAAAATCATATT+TGG + chr4.3:86046121-86046140 None:intergenic 15.0%
!! TACAAAATTAAATGAATATG+AGG + chr4.3:86046027-86046046 None:intergenic 15.0%
!! TACAAATTAATACAGAAAAA+AGG + chr4.3:86046326-86046345 None:intergenic 15.0%
!! TTTGCAAATTAATACAAATA+AGG + chr4.3:86046480-86046499 None:intergenic 15.0%
!!! AAATAAAAACTCAGTTATTT+TGG + chr4.3:86046516-86046535 None:intergenic 15.0%
!!! ATTGTTGACTTTAATTTTTA+TGG - chr4.3:86047314-86047333 MS.gene60217:intron 15.0%
!!! CATATTCATTTAATTTTGTA+TGG - chr4.3:86046027-86046046 MS.gene60217:intron 15.0%
!!! TCTAAAATCATATTTATCAA+TGG + chr4.3:86047899-86047918 None:intergenic 15.0%
!!! TCTAGTTTAAATATAGTTTT+TGG - chr4.3:86048428-86048447 MS.gene60217:CDS 15.0%
!!! TTTTTTTGGTGAAAAATTAA+GGG - chr4.3:86048129-86048148 MS.gene60217:intron 15.0%
!!! TTTTTTTTGGTGAAAAATTA+AGG - chr4.3:86048128-86048147 MS.gene60217:intron 15.0%
!! AGAAAGATATCAACAATAAT+AGG + chr4.3:86046672-86046691 None:intergenic 20.0%
!! ATAAGCATATAAAGATAGAA+AGG + chr4.3:86048503-86048522 None:intergenic 20.0%
!! CAAAATTAAATGAATATGAG+GGG + chr4.3:86046025-86046044 None:intergenic 20.0%
!! GAAAGATATCAACAATAATA+GGG + chr4.3:86046671-86046690 None:intergenic 20.0%
!! TACATCAAGAAAATTAAATG+AGG + chr4.3:86045975-86045994 None:intergenic 20.0%
!! TGAAAATAAAGTTTAGCTAT+TGG + chr4.3:86048717-86048736 None:intergenic 20.0%
!! TGCTAAAAATATAGTAACAT+AGG + chr4.3:86047083-86047102 None:intergenic 20.0%
!!! AATAATGTCTCATGTTATTT+TGG - chr4.3:86046346-86046365 MS.gene60217:intron 20.0%
!!! AATAGCTAAACTTTATTTTC+AGG - chr4.3:86048716-86048735 MS.gene60217:CDS 20.0%
!!! ATTTGGTAGTCTAAAATATA+AGG + chr4.3:86046104-86046123 None:intergenic 20.0%
!!! TATTTTGAGAGCATTTAATA+AGG - chr4.3:86046060-86046079 MS.gene60217:intron 20.0%
!!! TGTTACTATATTTTTAGCAT+TGG - chr4.3:86047084-86047103 MS.gene60217:intron 20.0%
!!! TTGGTTAATAAAAGTATCAA+TGG + chr4.3:86048698-86048717 None:intergenic 20.0%
!!! TTTGTTTAAATGCATTTTGT+TGG - chr4.3:86046259-86046278 MS.gene60217:intron 20.0%
!!! TTTTGACAAAAATAACAACT+TGG + chr4.3:86046802-86046821 None:intergenic 20.0%
! AAATCATATTTATCAATGGC+AGG + chr4.3:86047895-86047914 None:intergenic 25.0%
! AATCATATTTATCAATGGCA+GGG + chr4.3:86047894-86047913 None:intergenic 25.0%
! ACTATCAATCCTACAAAATT+CGG + chr4.3:86047263-86047282 None:intergenic 25.0%
! AGTTTCAAGATCTTGTAAAT+TGG - chr4.3:86047588-86047607 MS.gene60217:intron 25.0%
! ATCATCTACATAAATTTCCA+TGG + chr4.3:86046590-86046609 None:intergenic 25.0%
! ATTATGCAAGATTATCTCTT+GGG - chr4.3:86047151-86047170 MS.gene60217:intron 25.0%
! ATTCAAAACACATCACAAAT+AGG + chr4.3:86046296-86046315 None:intergenic 25.0%
! CAATCTCTTACTTAATACTA+TGG + chr4.3:86047201-86047220 None:intergenic 25.0%
! CCTATAATCATAGTTATCAA+TGG + chr4.3:86047985-86048004 None:intergenic 25.0%
! CTAATGACATTCAACAAAAA+TGG + chr4.3:86046385-86046404 None:intergenic 25.0%
! GGTTAACTACTATTCATAAT+AGG + chr4.3:86045954-86045973 None:intergenic 25.0%
! GTTTCAAGATCTTGTAAATT+GGG - chr4.3:86047589-86047608 MS.gene60217:intron 25.0%
! TATTATGCAAGATTATCTCT+TGG - chr4.3:86047150-86047169 MS.gene60217:intron 25.0%
! TCATCTACATAAATTTCCAT+GGG + chr4.3:86046589-86046608 None:intergenic 25.0%
!! AAAACAGCAACTTTTATATC+TGG + chr4.3:86045487-86045506 None:intergenic 25.0%
!! ATTTAATGTAGTGCTGAAAA+AGG + chr4.3:86046721-86046740 None:intergenic 25.0%
!! CCATTGATAACTATGATTAT+AGG - chr4.3:86047982-86048001 MS.gene60217:intron 25.0%
!! GATCCAACAATTTTATAATG+GGG - chr4.3:86047694-86047713 MS.gene60217:intron 25.0%
!! GGATCCAACAATTTTATAAT+GGG - chr4.3:86047693-86047712 MS.gene60217:intron 25.0%
!! TCTATCATTTTCATTGAAAC+TGG - chr4.3:86048650-86048669 MS.gene60217:CDS 25.0%
!! TGGATCCAACAATTTTATAA+TGG - chr4.3:86047692-86047711 MS.gene60217:intron 25.0%
!! TTGTAGGATTGATAGTAAAA+TGG - chr4.3:86047267-86047286 MS.gene60217:intron 25.0%
!!! AAAAAGTATCTTACTGTATG+TGG + chr4.3:86048796-86048815 None:intergenic 25.0%
!!! AGAGATTGCGTTTTTTATAT+TGG - chr4.3:86047213-86047232 MS.gene60217:intron 25.0%
!!! ATAAAAGTTGCTGTTTTCTA+TGG - chr4.3:86045490-86045509 MS.gene60217:CDS 25.0%
!!! GCTTTATAAAGGTTTTATCT+TGG - chr4.3:86049034-86049053 MS.gene60217:CDS 25.0%
!!! TACTTTTTCGTAGTAGTTTA+TGG - chr4.3:86048808-86048827 MS.gene60217:intron 25.0%
AATCATAGTTATCAATGGCA+GGG + chr4.3:86047980-86047999 None:intergenic 30.0%
ACAAGTGTGATGCTTTATAA+AGG - chr4.3:86049023-86049042 MS.gene60217:CDS 30.0%
ACATCATCACTTGTTTATCA+TGG + chr4.3:86045821-86045840 None:intergenic 30.0%
ATCATCACTTGTTTATCATG+GGG + chr4.3:86045819-86045838 None:intergenic 30.0%
ATTCATTGAGAAAAGTTACC+AGG - chr4.3:86047946-86047965 MS.gene60217:intron 30.0%
CAAAACAGTTCTTGCATATA+TGG + chr4.3:86046184-86046203 None:intergenic 30.0%
CAGAATGTATGCAAATTGAT+GGG + chr4.3:86046874-86046893 None:intergenic 30.0%
CAGCATATAAAACATATACC+CGG + chr4.3:86047027-86047046 None:intergenic 30.0%
CATCATCACTTGTTTATCAT+GGG + chr4.3:86045820-86045839 None:intergenic 30.0%
GGAAATTTATGTAGATGATG+AGG - chr4.3:86046591-86046610 MS.gene60217:intron 30.0%
TAATCATAGTTATCAATGGC+AGG + chr4.3:86047981-86048000 None:intergenic 30.0%
TAATGTGATGCGAAAATAAC+AGG + chr4.3:86046230-86046249 None:intergenic 30.0%
TATAAAGATAGAAAGGACAG+AGG + chr4.3:86048496-86048515 None:intergenic 30.0%
TCACCCCATTATAAAATTGT+TGG + chr4.3:86047700-86047719 None:intergenic 30.0%
TCAGTCTTAATAAACCAAGA+TGG - chr4.3:86047752-86047771 MS.gene60217:intron 30.0%
TGCACAAAGTTTATTAGAAC+TGG - chr4.3:86048850-86048869 MS.gene60217:intron 30.0%
TGGTGACCTTCAATTATAAT+GGG - chr4.3:86047507-86047526 MS.gene60217:CDS 30.0%
TTACAAGATCTTGAAACTTC+TGG + chr4.3:86047586-86047605 None:intergenic 30.0%
! AAAGTATCAATGGCCAAAAA+GGG + chr4.3:86048688-86048707 None:intergenic 30.0%
! AAATGGACTTATGTCTGATT+AGG - chr4.3:86047284-86047303 MS.gene60217:intron 30.0%
! AGTTCTTAATCAGGTTAGTT+AGG - chr4.3:86048612-86048631 MS.gene60217:CDS 30.0%
! ATACGTTTGTGATAGTGTAT+TGG + chr4.3:86045386-86045405 None:intergenic 30.0%
! ATCATTTTCATTGAAACTGG+AGG - chr4.3:86048653-86048672 MS.gene60217:CDS 30.0%
! ATGAATATGAGGGGTAATTT+TGG + chr4.3:86046016-86046035 None:intergenic 30.0%
! TACTGAACTTTATTTGTACG+AGG + chr4.3:86048173-86048192 None:intergenic 30.0%
! TAGTTATCAATTGTGGATTG+TGG - chr4.3:86047660-86047679 MS.gene60217:intron 30.0%
! TCAATGCTTTAAGCTGATTT+AGG - chr4.3:86046965-86046984 MS.gene60217:intron 30.0%
! TCATTTTCATTGAAACTGGA+GGG - chr4.3:86048654-86048673 MS.gene60217:CDS 30.0%
! TGATAGTGTATTGGAAACAA+AGG + chr4.3:86045377-86045396 None:intergenic 30.0%
! TGCACGAATAGTCAATATTT+TGG + chr4.3:86047127-86047146 None:intergenic 30.0%
! TTAATAAGGCGAAGTTGATT+AGG - chr4.3:86046074-86046093 MS.gene60217:intron 30.0%
!! AAAAGTATCAATGGCCAAAA+AGG + chr4.3:86048689-86048708 None:intergenic 30.0%
!! TAGGTTTGAGGTTGATATAA+AGG - chr4.3:86048746-86048765 MS.gene60217:CDS 30.0%
!!! ATAGTTTTTGGCTTGTAGTA+TGG - chr4.3:86048440-86048459 MS.gene60217:CDS 30.0%
!!! TTTATTTTCAGGTGCAATCT+AGG - chr4.3:86048727-86048746 MS.gene60217:CDS 30.0%
AAGACCATGAAGAGTCAATT+TGG + chr4.3:86046617-86046636 None:intergenic 35.0%
AAGATCTTGAAACTTCTGGA+AGG + chr4.3:86047582-86047601 None:intergenic 35.0%
AAGTATCTTACTGTATGTGG+AGG + chr4.3:86048793-86048812 None:intergenic 35.0%
ATACCTAAACATATCCAGTC+CGG + chr4.3:86047537-86047556 None:intergenic 35.0%
ATCATAGTTATCAATGGCAG+GGG + chr4.3:86047979-86047998 None:intergenic 35.0%
ATCCATTTCCTGTTTGAAGA+GGG - chr4.3:86047622-86047641 MS.gene60217:CDS 35.0%
ATGCTGGATGTCTGTAAAAT+GGG - chr4.3:86047041-86047060 MS.gene60217:intron 35.0%
CCAGAATGTATGCAAATTGA+TGG + chr4.3:86046875-86046894 None:intergenic 35.0%
CCATCAATTTGCATACATTC+TGG - chr4.3:86046872-86046891 MS.gene60217:intron 35.0%
CTAGGCATAGTTATCAATTG+TGG - chr4.3:86047653-86047672 MS.gene60217:intron 35.0%
GACAAATTGCTGATGAGATT+AGG + chr4.3:86047440-86047459 None:intergenic 35.0%
GGTGACCTTCAATTATAATG+GGG - chr4.3:86047508-86047527 MS.gene60217:CDS 35.0%
GTGACCTTCAATTATAATGG+GGG - chr4.3:86047509-86047528 MS.gene60217:intron 35.0%
GTGGTGACCTTCAATTATAA+TGG - chr4.3:86047506-86047525 MS.gene60217:CDS 35.0%
TAACTTACAGGAAAGGGATT+GGG + chr4.3:86047355-86047374 None:intergenic 35.0%
TATCCATTTCCTGTTTGAAG+AGG - chr4.3:86047621-86047640 MS.gene60217:CDS 35.0%
TATGCTGGATGTCTGTAAAA+TGG - chr4.3:86047040-86047059 MS.gene60217:intron 35.0%
TCATTTGCCAAGCAAAATCT+GGG - chr4.3:86045161-86045180 MS.gene60217:intron 35.0%
TCCTTATGATTGAGACAGTT+GGG + chr4.3:86045913-86045932 None:intergenic 35.0%
TGAATTTCTTGCAAACAGGT+CGG + chr4.3:86045869-86045888 None:intergenic 35.0%
TGCATGAATTTCTTGCAAAC+AGG + chr4.3:86045873-86045892 None:intergenic 35.0%
TGGCTGGTAACTATTCTAAA+TGG + chr4.3:86047865-86047884 None:intergenic 35.0%
TTAACTTACAGGAAAGGGAT+TGG + chr4.3:86047356-86047375 None:intergenic 35.0%
TTCAGATTGAAGCGATACAA+GGG - chr4.3:86048247-86048266 MS.gene60217:intron 35.0%
TTCCTTATGATTGAGACAGT+TGG + chr4.3:86045914-86045933 None:intergenic 35.0%
TTTCAGATTGAAGCGATACA+AGG - chr4.3:86048246-86048265 MS.gene60217:intron 35.0%
! AGATCTTGAAACTTCTGGAA+GGG + chr4.3:86047581-86047600 None:intergenic 35.0%
! AGTGTTTATGTGGCAGTTTT+TGG - chr4.3:86046157-86046176 MS.gene60217:intron 35.0%
! ATCTTGTGCTTTAAGTAGGT+TGG - chr4.3:86047413-86047432 MS.gene60217:CDS 35.0%
! ATCTTTTCTCATGTCTGCAA+AGG + chr4.3:86045772-86045791 None:intergenic 35.0%
! CATGATCTTGTGCTTTAAGT+AGG - chr4.3:86047409-86047428 MS.gene60217:CDS 35.0%
! CATTTTCATTGAAACTGGAG+GGG - chr4.3:86048655-86048674 MS.gene60217:CDS 35.0%
! CGGGTATATGTTTTATATGC+TGG - chr4.3:86047025-86047044 MS.gene60217:intron 35.0%
! TGACCTTCAATTATAATGGG+GGG - chr4.3:86047510-86047529 MS.gene60217:intron 35.0%
!! ATTACTTTTGTGTCTTCTGC+TGG - chr4.3:86048580-86048599 MS.gene60217:CDS 35.0%
!! TGACTCTGAAGTTCTTAATC+AGG - chr4.3:86048603-86048622 MS.gene60217:CDS 35.0%
!!! AAAGTTGCTGTTTTCTATGG+TGG - chr4.3:86045493-86045512 MS.gene60217:CDS 35.0%
!!! CGTTCTAAGATGTTTTGTTC+CGG - chr4.3:86047005-86047024 MS.gene60217:intron 35.0%
!!! GTTCTAAGATGTTTTGTTCC+GGG - chr4.3:86047006-86047025 MS.gene60217:intron 35.0%
!!! TGCATTTCGTTGATTTTGTG+TGG - chr4.3:86046423-86046442 MS.gene60217:intron 35.0%
!!! TGCATTTTGTTGGTTTTGTG+CGG - chr4.3:86046269-86046288 MS.gene60217:intron 35.0%
AAAAGTAATTGCAAGCCCCT+TGG + chr4.3:86048569-86048588 None:intergenic 40.0%
AAACAACACCCTCTTCAAAC+AGG + chr4.3:86047633-86047652 None:intergenic 40.0%
AAGGTCCTTATCTCTAGTCA+GGG + chr4.3:86045599-86045618 None:intergenic 40.0%
AGATAGGTATCAGCAGAATC+AGG + chr4.3:86048370-86048389 None:intergenic 40.0%
AGTACATAAGACTACGCATG+TGG + chr4.3:86046944-86046963 None:intergenic 40.0%
ATGCTTATCGACATCTGAAC+TGG - chr4.3:86048515-86048534 MS.gene60217:intron 40.0%
ATTTATCAATGGCAGGGTAG+CGG + chr4.3:86047888-86047907 None:intergenic 40.0%
CAGGAGTTGGAACAATAAGA+AGG + chr4.3:86046211-86046230 None:intergenic 40.0%
CTACATAAATTTCCATGGGC+TGG + chr4.3:86046585-86046604 None:intergenic 40.0%
CTGGAATGTCACAAGAAGAA+AGG - chr4.3:86046891-86046910 MS.gene60217:intron 40.0%
GATGCGAAAATAACAGGAGT+TGG + chr4.3:86046224-86046243 None:intergenic 40.0%
GATGTCTGTAAAATGGGACA+TGG - chr4.3:86047047-86047066 MS.gene60217:intron 40.0%
GGAGGTTAACTTACAGGAAA+GGG + chr4.3:86047361-86047380 None:intergenic 40.0%
GGCACTGCAGTAAAAACAAA+AGG + chr4.3:86049075-86049094 None:intergenic 40.0%
GTCATTTGCCAAGCAAAATC+TGG - chr4.3:86045160-86045179 MS.gene60217:intron 40.0%
GTGTTCCAACAACAATGTGT+GGG + chr4.3:86045561-86045580 None:intergenic 40.0%
TCATGTCTGCAAAGGTCAAA+TGG + chr4.3:86045764-86045783 None:intergenic 40.0%
TCCCAACTGTCTCAATCATA+AGG - chr4.3:86045909-86045928 MS.gene60217:CDS 40.0%
TCTGTAAAATGGGACATGGT+TGG - chr4.3:86047051-86047070 MS.gene60217:intron 40.0%
TGATGAATGTGACAAGATGC+TGG - chr4.3:86045648-86045667 MS.gene60217:CDS 40.0%
TGCTGATACCTATCTTCACA+GGG - chr4.3:86048375-86048394 MS.gene60217:CDS 40.0%
TTATCGACATCTGAACTGGT+AGG - chr4.3:86048519-86048538 MS.gene60217:intron 40.0%
TTCAGCTTACGATTCTTCTC+TGG + chr4.3:86046752-86046771 None:intergenic 40.0%
TTGACTACTTCCAGCTTAGA+AGG - chr4.3:86047479-86047498 MS.gene60217:CDS 40.0%
TTTGCTTGTCAGATCTGAAG+TGG + chr4.3:86047818-86047837 None:intergenic 40.0%
! AAGGACAGAGGTCTTTTACA+AGG + chr4.3:86048484-86048503 None:intergenic 40.0%
! ACATTGTTGTTGGAACACCT+GGG - chr4.3:86045563-86045582 MS.gene60217:intron 40.0%
! ACCTTGGTTTGAAGAATGTG+AGG - chr4.3:86045614-86045633 MS.gene60217:intron 40.0%
! AGTTCTCTGCCGAATTTTGT+AGG - chr4.3:86047251-86047270 MS.gene60217:intron 40.0%
! CACAAGAGAACTAGCTTATC+AGG - chr4.3:86045270-86045289 MS.gene60217:intron 40.0%
! GATAAGCTAGTTCTCTTGTG+TGG + chr4.3:86045270-86045289 None:intergenic 40.0%
! TTCAATTATAATGGGGGGAC+CGG - chr4.3:86047515-86047534 MS.gene60217:intron 40.0%
! TTCCAGCTTAGAAGGTATAG+TGG - chr4.3:86047487-86047506 MS.gene60217:CDS 40.0%
! TTGCTTGTCAGATCTGAAGT+GGG + chr4.3:86047817-86047836 None:intergenic 40.0%
!! TTGAAGAGGGTGTTGTTTCT+AGG - chr4.3:86047635-86047654 MS.gene60217:CDS 40.0%
AACCCGCAATTAAGGAGACA+AGG + chr4.3:86045697-86045716 None:intergenic 45.0%
AATCCCCCTCCCTACTAAAT+CGG + chr4.3:86048308-86048327 None:intergenic 45.0%
AATTGATCCTGTTCCTGGTC+AGG - chr4.3:86045225-86045244 MS.gene60217:intron 45.0%
ACTATGCAAACATGCACAGG+AGG + chr4.3:86047383-86047402 None:intergenic 45.0%
AGACCAGCGTAGTGTTTATG+TGG - chr4.3:86046147-86046166 MS.gene60217:intron 45.0%
AGATTTGCCACGAAGTTGAG+AGG - chr4.3:86045446-86045465 MS.gene60217:intron 45.0%
AGCACTTACCCTGTGAAGAT+AGG + chr4.3:86048386-86048405 None:intergenic 45.0%
CAAGGTCCTTATCTCTAGTC+AGG + chr4.3:86045600-86045619 None:intergenic 45.0%
CACCACTATACCTTCTAAGC+TGG + chr4.3:86047492-86047511 None:intergenic 45.0%
CACCCTCTTCAAACAGGAAA+TGG + chr4.3:86047627-86047646 None:intergenic 45.0%
CAGCAAATTGATCCTGTTCC+TGG - chr4.3:86045220-86045239 MS.gene60217:intron 45.0%
CATTGAAACTGGAGGGGAAT+AGG - chr4.3:86048661-86048680 MS.gene60217:CDS 45.0%
CCTCCTTGTCTCCTTAATTG+CGG - chr4.3:86045691-86045710 MS.gene60217:CDS 45.0%
CGATACAAGGGCTTCAAAGA+GGG - chr4.3:86048259-86048278 MS.gene60217:intron 45.0%
CTATGCAAACATGCACAGGA+GGG + chr4.3:86047382-86047401 None:intergenic 45.0%
CTCCTTGTCTCCTTAATTGC+GGG - chr4.3:86045692-86045711 MS.gene60217:CDS 45.0%
CTCGACAAAACCCGCAATTA+AGG + chr4.3:86045705-86045724 None:intergenic 45.0%
CTGAAACAGACATGTCGTCA+TGG + chr4.3:86048232-86048251 None:intergenic 45.0%
CTGACTAGAGATAAGGACCT+TGG - chr4.3:86045598-86045617 MS.gene60217:intron 45.0%
CTGCTGATACCTATCTTCAC+AGG - chr4.3:86048374-86048393 MS.gene60217:CDS 45.0%
GACAAGATGCTGGAATCACT+TGG - chr4.3:86045658-86045677 MS.gene60217:CDS 45.0%
GATACAAGGGCTTCAAAGAG+GGG - chr4.3:86048260-86048279 MS.gene60217:intron 45.0%
GCCTCACATTCTTCAAACCA+AGG + chr4.3:86045618-86045637 None:intergenic 45.0%
GGGAGGTTAACTTACAGGAA+AGG + chr4.3:86047362-86047381 None:intergenic 45.0%
GGTCCCCCCATTATAATTGA+AGG + chr4.3:86047516-86047535 None:intergenic 45.0%
GGTGTTCCAACAACAATGTG+TGG + chr4.3:86045562-86045581 None:intergenic 45.0%
TCAGGTGCAATCTAGGTTTG+AGG - chr4.3:86048734-86048753 MS.gene60217:CDS 45.0%
TCCTGTGCATGTTTGCATAG+TGG - chr4.3:86047382-86047401 MS.gene60217:CDS 45.0%
TCTCAACTTCGTGGCAAATC+TGG + chr4.3:86045447-86045466 None:intergenic 45.0%
TCTTGCCCTGACTAGAGATA+AGG - chr4.3:86045591-86045610 MS.gene60217:intron 45.0%
TGAGCCAGTAATCCGACATA+TGG - chr4.3:86047780-86047799 MS.gene60217:intron 45.0%
TGGAGTCTAACTAGTTGCTC+TGG + chr4.3:86047845-86047864 None:intergenic 45.0%
TTGCCAAGCAAAATCTGGGA+TGG - chr4.3:86045165-86045184 MS.gene60217:intron 45.0%
! AATCTTTTTCCTCCAGCCCA+TGG - chr4.3:86046570-86046589 MS.gene60217:intron 45.0%
! CACATTGTTGTTGGAACACC+TGG - chr4.3:86045562-86045581 MS.gene60217:intron 45.0%
! CTATGGTGGTGTCAACATCA+AGG - chr4.3:86045507-86045526 MS.gene60217:intron 45.0%
! CTTGTTCAGGTGCGTTTTGT+GGG - chr4.3:86046634-86046653 MS.gene60217:intron 45.0%
! GACAGAGGTCTTTTACAAGG+AGG + chr4.3:86048481-86048500 None:intergenic 45.0%
! GAGGCCAAATTGACTCTTCA+TGG - chr4.3:86046610-86046629 MS.gene60217:intron 45.0%
! GTTTTCAGCTACACTCAGCA+AGG - chr4.3:86045839-86045858 MS.gene60217:intron 45.0%
! TCGTGGCAACCGATTTAGTA+GGG - chr4.3:86048296-86048315 MS.gene60217:intron 45.0%
! TCTTGTTCAGGTGCGTTTTG+TGG - chr4.3:86046633-86046652 MS.gene60217:intron 45.0%
! TGCCAAGCAAAATCTGGGAT+GGG - chr4.3:86045166-86045185 MS.gene60217:intron 45.0%
! TGCTTGTCAGATCTGAAGTG+GGG + chr4.3:86047816-86047835 None:intergenic 45.0%
! TTCCCATCCCAGATTTTGCT+TGG + chr4.3:86045171-86045190 None:intergenic 45.0%
!! CATAAATTTCCATGGGCTGG+AGG + chr4.3:86046582-86046601 None:intergenic 45.0%
!! GACTCTTCATGGTCTTGTTC+AGG - chr4.3:86046621-86046640 MS.gene60217:intron 45.0%
!! GTGGAGGTGTCAATTTGTTC+AGG + chr4.3:86048777-86048796 None:intergenic 45.0%
AGCTGGTAGATTTGGCACCA+AGG - chr4.3:86048549-86048568 MS.gene60217:intron 50.0%
AGTTATCAATGGCAGGGGAG+TGG + chr4.3:86047974-86047993 None:intergenic 50.0%
CAGGAGGGAGGTTAACTTAC+AGG + chr4.3:86047367-86047386 None:intergenic 50.0%
CAGGCCATATGTCGGATTAC+TGG + chr4.3:86047787-86047806 None:intergenic 50.0%
CCGCAATTAAGGAGACAAGG+AGG + chr4.3:86045694-86045713 None:intergenic 50.0%
CGACATCTGAACTGGTAGGT+GGG - chr4.3:86048523-86048542 MS.gene60217:intron 50.0%
CTGCCACATAAACACTACGC+TGG + chr4.3:86046153-86046172 None:intergenic 50.0%
CTGGTAGATTTGGCACCAAG+GGG - chr4.3:86048551-86048570 MS.gene60217:intron 50.0%
GAGTGCCCACACATTGTTGT+TGG - chr4.3:86045553-86045572 MS.gene60217:intron 50.0%
GCCACTATGCAAACATGCAC+AGG + chr4.3:86047386-86047405 None:intergenic 50.0%
GCGATACAAGGGCTTCAAAG+AGG - chr4.3:86048258-86048277 MS.gene60217:intron 50.0%
GCTGGTAGATTTGGCACCAA+GGG - chr4.3:86048550-86048569 MS.gene60217:intron 50.0%
GTGGGTAGAGCTGGTAGATT+TGG - chr4.3:86048541-86048560 MS.gene60217:intron 50.0%
TCGACATCTGAACTGGTAGG+TGG - chr4.3:86048522-86048541 MS.gene60217:intron 50.0%
TGCTGAACCTCTCAACTTCG+TGG + chr4.3:86045456-86045475 None:intergenic 50.0%
! ACTGGCTCAACTAGCCATCT+TGG + chr4.3:86047769-86047788 None:intergenic 50.0%
! CAACCGATTTAGTAGGGAGG+GGG - chr4.3:86048302-86048321 MS.gene60217:intron 50.0%
! CTCGTGGCAACCGATTTAGT+AGG - chr4.3:86048295-86048314 MS.gene60217:intron 50.0%
! GAGGGGAATAGGACCCTTTT+TGG - chr4.3:86048672-86048691 MS.gene60217:CDS 50.0%
! GCAACCGATTTAGTAGGGAG+GGG - chr4.3:86048301-86048320 MS.gene60217:intron 50.0%
! GGACCGGACTGGATATGTTT+AGG - chr4.3:86047531-86047550 MS.gene60217:intron 50.0%
! GGCAACCGATTTAGTAGGGA+GGG - chr4.3:86048300-86048319 MS.gene60217:intron 50.0%
! GTGGATTGTGGCACAGTAAC+TGG - chr4.3:86047672-86047691 MS.gene60217:intron 50.0%
! TGGCAACCGATTTAGTAGGG+AGG - chr4.3:86048299-86048318 MS.gene60217:intron 50.0%
! TTGTTGTTGGAACACCTGGG+AGG - chr4.3:86045566-86045585 MS.gene60217:intron 50.0%
AACTGGTAGGTGGGTAGAGC+TGG - chr4.3:86048532-86048551 MS.gene60217:intron 55.0%
GGCTTGGACAGGCCATATGT+CGG + chr4.3:86047795-86047814 None:intergenic 55.0%
GGGGAAGCAGAGAATTCTCG+TGG - chr4.3:86048279-86048298 MS.gene60217:intron 55.0%
TGCAAACATGCACAGGAGGG+AGG + chr4.3:86047379-86047398 None:intergenic 55.0%
! ACAAGTGCTGCAACCTGACC+AGG + chr4.3:86045241-86045260 None:intergenic 55.0%
! ATCTGAAGTGGGGCTTGGAC+AGG + chr4.3:86047806-86047825 None:intergenic 55.0%
! GTCAGATCTGAAGTGGGGCT+TGG + chr4.3:86047811-86047830 None:intergenic 55.0%
! TATCAATGGCAGGGTAGCGG+TGG + chr4.3:86047885-86047904 None:intergenic 55.0%
! TTATAATGGGGGGACCGGAC+TGG - chr4.3:86047520-86047539 MS.gene60217:intron 55.0%
GCTGCAACCTGACCAGGAAC+AGG + chr4.3:86045235-86045254 None:intergenic 60.0%
GTCAGGGCAAGAATCCTCCC+AGG + chr4.3:86045583-86045602 None:intergenic 60.0%
AATGGCAGGGGAGTGGTGCC+TGG + chr4.3:86047967-86047986 None:intergenic 65.0%
! AATGGCAGGGTAGCGGTGGC+TGG + chr4.3:86047881-86047900 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 86045154 86049101 86045154 ID=MS.gene60217
chr4.3 mRNA 86045154 86049101 86045154 ID=MS.gene60217.t1;Parent=MS.gene60217
chr4.3 exon 86048964 86049101 86048964 ID=MS.gene60217.t1.exon1;Parent=MS.gene60217.t1
chr4.3 CDS 86048964 86049101 86048964 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86048576 86048807 86048576 ID=MS.gene60217.t1.exon2;Parent=MS.gene60217.t1
chr4.3 CDS 86048576 86048807 86048576 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86048359 86048477 86048359 ID=MS.gene60217.t1.exon3;Parent=MS.gene60217.t1
chr4.3 CDS 86048359 86048477 86048359 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86047613 86047669 86047613 ID=MS.gene60217.t1.exon4;Parent=MS.gene60217.t1
chr4.3 CDS 86047613 86047669 86047613 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86047343 86047527 86047343 ID=MS.gene60217.t1.exon5;Parent=MS.gene60217.t1
chr4.3 CDS 86047343 86047527 86047343 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86045859 86046003 86045859 ID=MS.gene60217.t1.exon6;Parent=MS.gene60217.t1
chr4.3 CDS 86045859 86046003 86045859 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86045631 86045714 86045631 ID=MS.gene60217.t1.exon7;Parent=MS.gene60217.t1
chr4.3 CDS 86045631 86045714 86045631 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86045454 86045517 86045454 ID=MS.gene60217.t1.exon8;Parent=MS.gene60217.t1
chr4.3 CDS 86045454 86045517 86045454 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
chr4.3 exon 86045154 86045164 86045154 ID=MS.gene60217.t1.exon9;Parent=MS.gene60217.t1
chr4.3 CDS 86045154 86045164 86045154 ID=cds.MS.gene60217.t1;Parent=MS.gene60217.t1
Gene Sequence

>MS.gene60217

ATGGACGTCATTTGCCAAGCAAAATCTGGGATGGGAAAGACTGCTGTTTTTGTTCTGTCAACACTGCAGCAAATTGATCCTGTTCCTGGTCAGGTTGCAGCACTTGTTCTTTGCCACACAAGAGAACTAGCTTATCAGGTATTTCTGCATTAATTTGAATGTAATATATCTCGAATGTTTTGCAAAAGCTATATGTTTGCTAATTGCTAATCTCGAATTACCTTTGTTTCCAATACACTATCACAAACGTATATATTCGCATGTGCATACTCAGCAAATGCTTCATTTGTCCAGATTTGCCACGAAGTTGAGAGGTTCAGCACATATCTTCCAGATATAAAAGTTGCTGTTTTCTATGGTGGTGTCAACATCAAGGTTCACAAAGATTTGCTCAAGAATGAGTGCCCACACATTGTTGTTGGAACACCTGGGAGGATTCTTGCCCTGACTAGAGATAAGGACCTTGGTTTGAAGAATGTGAGGCATTTTATACTTGATGAATGTGACAAGATGCTGGAATCACTTGGTCTATTTCTTCCTCCTTGTCTCCTTAATTGCGGGTTTTGTCGAGTTTGTATATGCGATTTCTTATTTCTTAATATATTTGCCATTTGACCTTTGCAGACATGAGAAAAGATGTGCAAGAGATTTTTAAATTGACTCCCCATGATAAACAAGTGATGATGTTTTCAGCTACACTCAGCAAGGAAATCCGACCTGTTTGCAAGAAATTCATGCAAGATGTAATTTTCTACTCCCAACTGTCTCAATCATAAGGAAAACAAACTAACTGCACACCTATTATGAATAGTAGTTAACCTCATTTAATTTTCTTGATGTAATAAAAATGATTGCATTTCCAAAATTACCCCTCATATTCATTTAATTTTGTATGGAATTAAATATTATTTTGAGAGCATTTAATAAGGCGAAGTTGATTAGGTTTTCCTTATATTTTAGACTACCAAATATGATTTTTTTTGCTTATATTTGAGACCAGCGTAGTGTTTATGTGGCAGTTTTTGGACCATATATGCAAGAACTGTTTTGTCATCCTTCTTATTGTTCCAACTCCTGTTATTTTCGCATCACATTATTGCCATATTTTGTTTAAATGCATTTTGTTGGTTTTGTGCGGTCCTATTTGTGATGTGTTTTGAATTTTTGATCCTTTTTTCTGTATTAATTTGTAAATAATGTCTCATGTTATTTTGGAATCACATCATTGCCATTTTTGTTGAATGTCATTAGTTTTTTTTCTTGTTGTAATGCATTTCGTTGATTTTGTGTGGTCATATTTGTGATAAGTTTTGACTTTTTGATCCTTATTTGTATTAATTTGCAAATATGTTTTATATACCAAAATAACTGAGTTTTTATTTTTTTAATGTATGTCATCATTATATTCTCATTCATAATCTTTTTCCTCCAGCCCATGGAAATTTATGTAGATGATGAGGCCAAATTGACTCTTCATGGTCTTGTTCAGGTGCGTTTTGTGGGCTTTATTTATTCCCTATTATTGTTGATATCTTTCTGTCTTCTAATTTTTACTTAATTGTCACCTTTTTCAGCACTACATTAAATTACAAGAGCCAGAGAAGAATCGTAAGCTGAATGATCTTCTTGATGCTCTTGACTTCAACCAAGTTGTTATTTTTGTCAAAAGTGTTAGCAGAGCAGCAGAATTGAATAAGTTGCTTGTTGAGTGTAACTTCCCATCAATTTGCATACATTCTGGAATGTCACAAGAAGAAAGGTATTTACACGTTCTTTTGAAATTAATGCCACATGCGTAGTCTTATGTACTCTCAATGCTTTAAGCTGATTTAGGATTTGATAGCATTGTAGCGTTCTAAGATGTTTTGTTCCGGGTATATGTTTTATATGCTGGATGTCTGTAAAATGGGACATGGTTGGTTTTTGCCTATGTTACTATATTTTTAGCATTGGTAAACATCGTGTTAAAACCAAAATATTGACTATTCGTGCATTATATTATGCAAGATTATCTCTTGGGCAGTTTTGAAAGCTGATGTAATTACCATAGTATTAAGTAAGAGATTGCGTTTTTTATATTGGTGTCATGCACATCACAGTTCTCTGCCGAATTTTGTAGGATTGATAGTAAAATGGACTTATGTCTGATTAGGTCTCTGCATTGTTGACTTTAATTTTTATGGTTGTAGCTTTATAAACCCAATCCCTTTCCTGTAAGTTAACCTCCCTCCTGTGCATGTTTGCATAGTGGCTTGCATGATCTTGTGCTTTAAGTAGGTTGGTCCTAATCTCATCAGCAATTTGTCTCTTTGCTTAGCAAGTCTGTTGACTACTTCCAGCTTAGAAGGTATAGTGGTGACCTTCAATTATAATGGGGGGACCGGACTGGATATGTTTAGGTATTTTGTTAAACAACTGTGACAACCCTTCCAGAAGTTTCAAGATCTTGTAAATTGGGAAAGATAGATATCCATTTCCTGTTTGAAGAGGGTGTTGTTTCTAGGCATAGTTATCAATTGTGGATTGTGGCACAGTAACTGGATCCAACAATTTTATAATGGGGTGACTACTGTTCTAATTTATTTTAATACATATAAATCAGTCTTAATAAACCAAGATGGCTAGTTGAGCCAGTAATCCGACATATGGCCTGTCCAAGCCCCACTTCAGATCTGACAAGCAAATAAACCAGAGCAACTAGTTAGACTCCATTTAGAATAGTTACCAGCCACCGCTACCCTGCCATTGATAAATATGATTTTAGAAAGATCTCTTGTTTCTTTTATTAGCATATTCATTGAGAAAAGTTACCAGGCACCACTCCCCTGCCATTGATAACTATGATTATAGGAAGATCTTCTCTTATTTCTTTTATTAGCATAGATTTATTTTCTCTTATTTGTACAAATCATTTTTATTGTTTTACTATTAAGACAGATTCTTTCTATTAATTTAAGGGTAAAAACTAATAGATTTTTTTTTGGTGAAAAATTAAGGGAATCTATGATAGCTGTGTCCTCGTACAAATAAAGTTCAGTAATTTTTCTCTTTTCAAGTTGCAGTCTTAATATTTTTCCATGACGACATGTCTGTTTCAGATTGAAGCGATACAAGGGCTTCAAAGAGGGGAAGCAGAGAATTCTCGTGGCAACCGATTTAGTAGGGAGGGGGATTGATATAGAGCGTGTCAATATAGTCATCAACTACGACATGCCTGATTCTGCTGATACCTATCTTCACAGGGTAAGTGCTTGATTGAACAAATTTTTATCAATCTAGTTTAAATATAGTTTTTGGCTTGTAGTATGGTATGCTTACTTGCTACCTCCTTGTAAAAGACCTCTGTCCTTTCTATCTTTATATGCTTATCGACATCTGAACTGGTAGGTGGGTAGAGCTGGTAGATTTGGCACCAAGGGGCTTGCAATTACTTTTGTGTCTTCTGCTGGTGACTCTGAAGTTCTTAATCAGGTTAGTTAGGAACTTAATTTTTTTTTCTATCATTTTCATTGAAACTGGAGGGGAATAGGACCCTTTTTGGCCATTGATACTTTTATTAACCAATAGCTAAACTTTATTTTCAGGTGCAATCTAGGTTTGAGGTTGATATAAAGGAGCTTCCTGAACAAATTGACACCTCCACATACAGTAAGATACTTTTTCGTAGTAGTTTATGGTTTTAATAGTTTGAGTAGATGCACAAAGTTTATTAGAACTGGAAGCATTGCATAAATGAAGTTTATCGCAACTGCAAGTGTGATGTTTGTATATAATAAAGTTGATCAGAACTGCAAATAGTGTTTGCATATAATAATACTTGAACATACATGAAATTGAAGCAGAAAATGTTGATTTCATTAATATTTTTCACAAGTGTGATGCTTTATAAAGGTTTTATCTTGGTCACAAATGTAACATCCTTTTGTTTTTACTGCAGTGCCTAATTGA
Protein sequence

>MS.gene60217.t1

MDVICQAKSGMGKTAVFVLSTLQQIDPVPGQVAALVLCHTRELAYQICHEVERFSTYLPDIKVAVFYGGVNIKVHKDLLKNECPHIVVGTPGRILALTRDKDLGLKNVRHFILDECDKMLESLDMRKDVQEIFKLTPHDKQVMMFSATLSKEIRPVCKKFMQDPMEIYVDDEAKLTLHGLVQHYIKLQEPEKNRKLNDLLDALDFNQVVIFVKSVSRAAELNKLLVECNFPSICIHSGMSQEERLKRYKGFKEGKQRILVATDLVGRGIDIERVNIVINYDMPDSADTYLHRVGRAGRFGTKGLAITFVSSAGDSEVLNQVQSRFEVDIKELPEQIDTSTYMPN