AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061316

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061316.t1 MTR_1g061970 74.292 424 108 1 2 424 68 491 0.0 642
MS.gene061316.t1 MTR_4g049910 72.406 424 100 5 2 424 43 450 0.0 591
MS.gene061316.t1 MTR_0427s0010 80.723 249 23 1 176 424 2 225 3.37e-138 395
MS.gene061316.t1 MTR_2g039960 34.988 403 225 13 14 411 41 411 2.46e-61 204
MS.gene061316.t1 MTR_4g125970 34.568 405 224 13 14 411 41 411 2.75e-60 202
MS.gene061316.t1 MTR_4g125970 34.568 405 224 13 14 411 58 428 4.54e-60 201
MS.gene061316.t1 MTR_2g099620 35.787 394 219 13 14 404 25 387 3.76e-59 198
MS.gene061316.t1 MTR_2g099620 35.859 396 216 14 14 404 41 403 5.36e-59 198
MS.gene061316.t1 MTR_8g018400 33.835 399 223 13 14 405 41 405 1.13e-57 195
MS.gene061316.t1 MTR_2g438110 32.570 393 236 10 14 405 34 398 9.15e-57 192
MS.gene061316.t1 MTR_7g076820 32.071 396 240 10 10 405 146 512 3.17e-53 186
MS.gene061316.t1 MTR_7g076800 31.818 396 241 10 10 405 137 503 1.09e-51 181
MS.gene061316.t1 MTR_7g076830 31.818 396 241 10 10 405 139 505 1.36e-51 181
MS.gene061316.t1 MTR_1g012420 28.750 400 254 9 4 398 35 408 1.38e-47 168
MS.gene061316.t1 MTR_4g010565 28.822 399 257 6 12 408 122 495 4.79e-47 169
MS.gene061316.t1 MTR_3g107420 28.974 390 248 8 12 398 45 408 7.55e-47 166
MS.gene061316.t1 MTR_8g018370 31.818 352 184 12 51 395 4 306 2.13e-37 139
MS.gene061316.t1 MTR_6g082080 30.075 399 238 14 14 404 10 375 3.26e-37 140
MS.gene061316.t1 MTR_2g066050 31.407 398 239 13 5 396 44 413 3.44e-37 142
MS.gene061316.t1 MTR_2g100230 30.469 384 220 13 11 383 98 445 7.59e-37 140
MS.gene061316.t1 MTR_7g056830 29.574 399 240 14 14 404 10 375 2.83e-36 138
MS.gene061316.t1 MTR_7g100820 30.423 378 231 11 31 405 150 498 3.98e-36 140
MS.gene061316.t1 MTR_1g095810 28.993 407 243 13 1 391 132 508 1.01e-34 136
MS.gene061316.t1 MTR_2g007000 30.601 366 209 12 34 385 523 857 1.13e-34 137
MS.gene061316.t1 MTR_1g026830 28.501 407 245 13 1 391 134 510 1.90e-34 135
MS.gene061316.t1 MTR_7g109720 29.255 376 235 11 14 383 232 582 2.33e-34 136
MS.gene061316.t1 MTR_1g046570 29.584 409 220 14 34 404 10 388 4.43e-34 131
MS.gene061316.t1 MTR_6g005550 28.499 393 244 13 1 385 152 515 5.50e-33 132
MS.gene061316.t1 MTR_4g105100 27.824 363 229 9 28 383 215 551 3.24e-32 129
MS.gene061316.t1 MTR_4g134170 28.784 403 219 11 14 383 287 654 9.14e-32 128
MS.gene061316.t1 MTR_2g041140 27.696 408 243 15 11 402 138 509 2.49e-31 127
MS.gene061316.t1 MTR_3g467550 27.569 399 252 12 14 400 117 490 4.76e-31 125
MS.gene061316.t1 MTR_3g049990 27.470 415 247 15 4 402 143 519 5.16e-31 126
MS.gene061316.t1 MTR_6g056080 30.287 383 216 16 40 403 128 478 6.10e-31 125
MS.gene061316.t1 MTR_1g102410 29.723 397 205 15 40 399 69 428 6.84e-31 125
MS.gene061316.t1 MTR_2g032630 27.632 380 238 13 14 385 496 846 1.35e-30 125
MS.gene061316.t1 MTR_6g005320 28.288 403 219 12 14 383 249 614 2.28e-30 124
MS.gene061316.t1 MTR_3g083690 27.638 398 241 15 5 380 137 509 2.29e-30 124
MS.gene061316.t1 MTR_3g449250 28.267 375 230 13 48 411 119 465 3.47e-30 123
MS.gene061316.t1 MTR_3g467550 27.895 380 238 11 33 400 104 459 3.67e-30 122
MS.gene061316.t1 MTR_3g449250 28.267 375 230 13 48 411 119 465 4.31e-30 122
MS.gene061316.t1 MTR_1g054630 27.708 397 244 12 4 380 144 517 5.77e-30 122
MS.gene061316.t1 MTR_5g027840 29.668 391 223 13 4 376 94 450 9.02e-30 121
MS.gene061316.t1 MTR_4g114120 27.200 375 229 11 23 376 148 499 1.84e-29 121
MS.gene061316.t1 MTR_8g027775 30.000 390 236 12 2 376 90 457 2.54e-29 120
MS.gene061316.t1 MTR_4g079320 28.133 391 219 16 15 385 20 368 1.01e-28 119
MS.gene061316.t1 MTR_0915s0010 29.066 289 192 7 31 319 5 280 1.82e-28 113
MS.gene061316.t1 MTR_5g090120 25.974 385 226 13 48 411 110 456 1.83e-28 118
MS.gene061316.t1 MTR_8g027770 29.716 387 235 12 5 376 192 556 4.87e-28 117
MS.gene061316.t1 MTR_8g027780 29.504 383 233 12 9 376 101 461 5.29e-28 116
MS.gene061316.t1 MTR_2g063080 26.327 452 246 18 6 398 102 525 3.23e-27 114
MS.gene061316.t1 MTR_3g467550 27.566 341 225 10 14 342 117 447 2.76e-25 107
MS.gene061316.t1 MTR_6g033070 27.154 383 239 13 14 385 140 493 9.86e-25 106
MS.gene061316.t1 MTR_6g039840 26.536 407 242 15 14 395 110 484 3.23e-24 105
MS.gene061316.t1 MTR_1g083130 25.721 451 244 16 14 404 26 445 6.49e-24 104
MS.gene061316.t1 MTR_4g112340 28.804 368 224 14 30 385 112 453 7.38e-24 104
MS.gene061316.t1 MTR_8g097000 27.366 391 241 12 11 383 285 650 8.06e-24 104
MS.gene061316.t1 MTR_3g071620 27.696 408 232 14 11 385 11 388 3.77e-23 102
MS.gene061316.t1 MTR_4g105100 26.899 316 212 8 31 339 218 521 1.05e-22 100
MS.gene061316.t1 MTR_5g092560 27.013 385 238 14 9 376 63 421 1.66e-21 97.8
MS.gene061316.t1 MTR_8g027780 29.275 345 221 11 9 338 101 437 6.41e-21 95.1
MS.gene061316.t1 MTR_5g069330 25.272 368 232 14 14 370 3 338 6.02e-20 91.7
MS.gene061316.t1 MTR_5g069330 25.272 368 232 14 14 370 7 342 6.93e-20 91.7
MS.gene061316.t1 MTR_3g107420 30.693 202 128 5 12 210 45 237 3.07e-18 85.5
MS.gene061316.t1 MTR_6g039840 25.994 327 197 12 85 395 11 308 1.06e-16 82.4
MS.gene061316.t1 MTR_5g077690 26.570 414 207 23 54 396 70 457 2.32e-15 78.2
MS.gene061316.t1 MTR_4g077370 31.754 211 127 9 4 210 243 440 7.31e-14 73.6
MS.gene061316.t1 MTR_4g459350 27.160 243 145 10 6 224 33 267 2.70e-13 70.1
MS.gene061316.t1 MTR_3g118120 23.892 406 256 15 4 379 122 504 8.93e-13 70.5
MS.gene061316.t1 MTR_8g032230 34.568 162 83 7 14 165 10 158 2.65e-12 65.5
MS.gene061316.t1 MTR_4g112320 26.621 293 183 11 101 385 3 271 6.38e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061316.t1 AT3G53110 59.906 424 159 5 2 423 80 494 6.72e-176 501
MS.gene061316.t1 AT1G72730 35.859 396 216 13 14 404 42 404 6.19e-62 206
MS.gene061316.t1 AT3G19760 33.249 394 234 10 13 405 35 400 2.34e-59 199
MS.gene061316.t1 AT3G13920 35.176 398 216 14 14 404 30 392 8.51e-59 197
MS.gene061316.t1 AT3G13920 35.176 398 216 14 14 404 40 402 1.32e-58 197
MS.gene061316.t1 AT3G13920 35.176 398 211 14 14 404 40 397 4.05e-58 196
MS.gene061316.t1 AT3G13920 35.433 381 206 12 14 386 40 388 1.20e-57 195
MS.gene061316.t1 AT3G13920 35.433 381 206 12 14 386 44 392 1.30e-57 195
MS.gene061316.t1 AT1G54270 34.925 398 217 14 14 404 40 402 7.66e-57 192
MS.gene061316.t1 AT1G54270 34.925 398 212 14 14 404 40 397 3.67e-56 191
MS.gene061316.t1 AT4G00660 31.566 396 242 10 10 405 128 494 8.36e-53 184
MS.gene061316.t1 AT4G00660 31.566 396 242 10 10 405 128 494 8.36e-53 184
MS.gene061316.t1 AT3G61240 32.323 396 239 10 10 405 121 487 1.24e-52 184
MS.gene061316.t1 AT3G61240 32.323 396 239 10 10 405 121 487 1.24e-52 184
MS.gene061316.t1 AT1G51380 31.807 393 239 11 14 405 23 387 2.51e-51 177
MS.gene061316.t1 AT2G45810 31.818 396 241 10 10 405 151 517 3.84e-51 180
MS.gene061316.t1 AT5G11170 27.250 400 262 8 12 408 45 418 3.90e-46 164
MS.gene061316.t1 AT5G11200 27.250 400 262 8 12 408 45 418 3.90e-46 164
MS.gene061316.t1 AT5G11170 27.451 357 233 6 55 408 2 335 2.63e-43 155
MS.gene061316.t1 AT5G11200 24.886 438 265 9 12 408 45 459 3.20e-40 149
MS.gene061316.t1 AT5G11200 25.658 456 257 13 12 408 45 477 1.14e-38 145
MS.gene061316.t1 AT3G09720 29.647 425 260 12 3 419 127 520 3.61e-37 142
MS.gene061316.t1 AT3G09720 29.647 425 260 12 3 419 127 520 3.61e-37 142
MS.gene061316.t1 AT4G33370 29.306 389 231 11 15 388 99 458 6.10e-37 142
MS.gene061316.t1 AT5G51280 29.064 406 244 13 1 391 134 510 3.85e-35 137
MS.gene061316.t1 AT1G20920 28.726 369 218 12 31 385 208 545 8.91e-35 137
MS.gene061316.t1 AT1G16280 30.825 412 245 16 10 412 55 435 9.51e-35 135
MS.gene061316.t1 AT3G01540 28.934 394 241 14 1 385 146 509 1.82e-34 135
MS.gene061316.t1 AT3G01540 28.934 394 241 14 1 385 146 509 1.94e-34 135
MS.gene061316.t1 AT3G01540 28.934 394 241 14 1 385 146 509 1.94e-34 135
MS.gene061316.t1 AT3G01540 28.934 394 241 14 1 385 146 509 1.94e-34 135
MS.gene061316.t1 AT5G63120 28.158 380 235 11 15 385 167 517 3.73e-34 134
MS.gene061316.t1 AT5G60990 29.340 409 238 15 14 404 10 385 4.55e-34 132
MS.gene061316.t1 AT3G22310 27.833 406 249 15 17 411 120 492 5.13e-34 134
MS.gene061316.t1 AT3G22310 27.833 406 249 15 17 411 120 492 5.67e-34 134
MS.gene061316.t1 AT3G06480 30.000 380 229 13 14 385 436 786 6.47e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G20920 28.804 368 217 12 32 385 547 883 7.88e-34 135
MS.gene061316.t1 AT1G55150 28.796 382 231 13 34 403 119 471 1.99e-33 131
MS.gene061316.t1 AT1G55150 28.272 382 233 12 34 403 144 496 2.12e-33 131
MS.gene061316.t1 AT5G08620 29.877 405 242 13 9 396 76 455 7.98e-33 130
MS.gene061316.t1 AT5G14610 28.173 394 244 14 1 385 157 520 2.40e-32 129
MS.gene061316.t1 AT5G14610 28.173 394 244 14 1 385 149 512 2.74e-32 129
MS.gene061316.t1 AT5G14610 27.919 394 245 13 1 385 149 512 2.83e-32 129
MS.gene061316.t1 AT2G47330 27.114 402 261 11 3 398 218 593 5.75e-32 129
MS.gene061316.t1 AT5G26742 28.495 372 225 11 54 412 47 390 7.95e-32 128
MS.gene061316.t1 AT5G26742 28.495 372 225 11 54 412 140 483 9.15e-32 128
MS.gene061316.t1 AT5G26742 28.495 372 225 11 54 412 140 483 9.16e-32 128
MS.gene061316.t1 AT4G16630 29.602 402 240 14 12 402 166 535 9.56e-32 128
MS.gene061316.t1 AT1G31970 30.311 386 218 14 9 376 112 464 2.00e-31 126
MS.gene061316.t1 AT5G14610 28.338 367 225 12 28 385 44 381 2.35e-31 125
MS.gene061316.t1 AT5G14610 28.338 367 225 12 28 385 44 381 2.35e-31 125
MS.gene061316.t1 AT3G22330 27.200 375 234 14 48 411 134 480 3.89e-31 126
MS.gene061316.t1 AT2G42520 28.894 398 238 13 4 380 150 523 4.36e-31 125
MS.gene061316.t1 AT2G42520 28.894 398 238 13 4 380 150 523 4.36e-31 125
MS.gene061316.t1 AT2G42520 28.894 398 238 13 4 380 150 523 4.36e-31 125
MS.gene061316.t1 AT1G28180 30.398 352 206 12 44 385 3 325 9.04e-31 122
MS.gene061316.t1 AT5G14610 28.177 362 220 12 34 385 249 580 1.38e-30 124
MS.gene061316.t1 AT5G08610 29.383 405 244 13 9 396 378 757 4.81e-30 123
MS.gene061316.t1 AT3G09620 29.076 368 187 14 32 385 415 722 8.73e-30 122
MS.gene061316.t1 AT3G09620 29.076 368 187 14 32 385 414 721 1.02e-29 122
MS.gene061316.t1 AT2G33730 27.665 394 235 12 14 383 314 681 1.04e-29 122
MS.gene061316.t1 AT3G58510 27.273 396 245 12 4 380 142 513 2.16e-29 120
MS.gene061316.t1 AT3G58510 27.273 396 245 12 4 380 142 513 2.16e-29 120
MS.gene061316.t1 AT3G58510 27.273 396 245 12 4 380 142 513 2.16e-29 120
MS.gene061316.t1 AT1G12770 26.846 447 250 16 7 399 104 527 4.94e-29 119
MS.gene061316.t1 AT1G12770 26.846 447 250 16 7 399 104 527 4.94e-29 119
MS.gene061316.t1 AT3G58570 27.136 398 245 13 4 380 137 510 1.02e-28 119
MS.gene061316.t1 AT5G63630 29.231 390 235 12 4 376 45 410 1.58e-28 117
MS.gene061316.t1 AT5G63630 29.231 390 235 12 4 376 239 604 2.99e-28 117
MS.gene061316.t1 AT1G77030 27.424 361 223 12 34 385 48 378 3.34e-28 117
MS.gene061316.t1 AT5G62190 30.214 374 217 15 40 399 122 465 5.04e-28 117
MS.gene061316.t1 AT4G09730 27.446 419 233 16 15 401 114 493 5.11e-28 117
MS.gene061316.t1 AT3G02065 29.199 387 236 12 14 390 111 469 4.44e-27 113
MS.gene061316.t1 AT3G02065 29.199 387 236 12 14 390 111 469 4.44e-27 113
MS.gene061316.t1 AT3G02065 28.902 346 213 9 54 390 11 332 5.86e-26 108
MS.gene061316.t1 AT1G51380 30.451 266 171 8 14 278 23 275 1.42e-25 105
MS.gene061316.t1 AT3G06480 29.518 332 211 12 14 337 436 752 5.31e-25 108
MS.gene061316.t1 AT3G18600 29.073 399 236 16 1 385 78 443 1.06e-24 107
MS.gene061316.t1 AT5G63120 27.554 323 210 10 15 328 167 474 2.45e-24 105
MS.gene061316.t1 AT5G63120 27.554 323 210 10 15 328 167 474 2.45e-24 105
MS.gene061316.t1 AT5G63120 27.554 323 210 10 15 328 167 474 2.45e-24 105
MS.gene061316.t1 AT5G63120 27.554 323 210 10 15 328 167 474 2.57e-24 105
MS.gene061316.t1 AT5G14610 27.434 339 219 12 1 329 157 478 1.69e-23 102
MS.gene061316.t1 AT2G40700 25.785 446 226 16 11 376 28 448 2.65e-21 96.7
MS.gene061316.t1 AT5G05450 26.291 426 267 13 11 415 12 411 6.80e-21 95.5
MS.gene061316.t1 AT5G65900 26.632 383 237 14 7 376 148 499 6.90e-21 95.5
MS.gene061316.t1 AT1G63250 26.575 365 229 9 36 383 350 692 8.00e-20 92.4
MS.gene061316.t1 AT1G51380 30.631 222 142 7 14 234 23 233 1.52e-19 89.0
MS.gene061316.t1 AT1G51380 30.631 222 142 7 14 234 23 233 1.98e-19 89.0
MS.gene061316.t1 AT4G34910 24.818 411 228 14 14 371 47 429 2.51e-19 90.9
MS.gene061316.t1 AT1G71370 25.445 393 248 12 38 415 40 402 4.19e-19 90.1
MS.gene061316.t1 AT2G07750 26.301 365 230 9 36 383 397 739 6.86e-18 86.7
MS.gene061316.t1 AT3G06980 25.061 407 243 18 7 379 368 746 9.89e-14 73.9

Find 85 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTAGAGAGTTAGCTATTC+AGG 0.181810 4.2:-51927282 MS.gene061316:intron
GTTGGTAGGGCCGGGCGATT+TGG 0.216013 4.2:-51925739 MS.gene061316:CDS
AAAGATTATATATTCAAAAT+AGG 0.248299 4.2:-51926163 MS.gene061316:CDS
CATAAAGCTCTTGTTGATTT+AGG 0.258960 4.2:-51926082 MS.gene061316:CDS
ATTATGTCTCAGGTGGTTAT+TGG 0.264228 4.2:-51927070 MS.gene061316:CDS
GATTCTTGAACCTCCATATA+AGG 0.271464 4.2:-51927417 MS.gene061316:CDS
AAGAATCATTGGCAAACTTA+TGG 0.276132 4.2:+51927433 None:intergenic
CTGTGACATGGTTGCCAAAC+TGG 0.287364 4.2:+51925507 None:intergenic
TCTTGTTGTTTAGGTTAATC+TGG 0.310627 4.2:-51925851 MS.gene061316:intron
AAAACCGCTTGTTTCGTACT+AGG 0.340407 4.2:-51927362 MS.gene061316:CDS
TTGGTAGGGCCGGGCGATTT+GGG 0.348110 4.2:-51925738 MS.gene061316:CDS
AATAACCACCTGAGACATAA+TGG 0.350333 4.2:+51927072 None:intergenic
ATGTTCAAGAGCATCTAGTA+TGG 0.382960 4.2:+51926045 None:intergenic
TCAAAGATTGAAAACCAGTT+TGG 0.383675 4.2:-51925521 MS.gene061316:CDS
TGTGCGATCAAATCCTTATA+TGG 0.390278 4.2:+51927404 None:intergenic
ATGCTCTTGAACATGAAGAT+AGG 0.393586 4.2:-51926036 MS.gene061316:CDS
TTTCACAGGGTTGGTAGGGC+CGG 0.394537 4.2:-51925748 MS.gene061316:CDS
GCACAAGCACCTAATGGTTC+TGG 0.408582 4.2:-51927386 MS.gene061316:CDS
AAAGTTCAATTCAAACTGTC+AGG 0.412003 4.2:-51926616 MS.gene061316:intron
TATGGAGGTTCAAGAATCAT+TGG 0.416364 4.2:+51927422 None:intergenic
TTGATCGCACAAGCACCTAA+TGG 0.438798 4.2:-51927392 MS.gene061316:CDS
GCTTGAGGATCATGCAAATT+CGG 0.438890 4.2:+51927320 None:intergenic
GGCCGGGCGATTTGGGCGCA+AGG 0.444627 4.2:-51925731 MS.gene061316:CDS
CTGTCAAGAATTTCGTTACA+AGG 0.452513 4.2:-51926300 MS.gene061316:CDS
TTCACAGGGTTGGTAGGGCC+GGG 0.453138 4.2:-51925747 MS.gene061316:CDS
CAGGTGGTTATTGGCACTCC+TGG 0.453907 4.2:-51927061 MS.gene061316:CDS
TCAAAATAGGAGAGAACATC+GGG 0.456602 4.2:-51926150 MS.gene061316:CDS
AAAGATGCTTTCAAGAAAGC+TGG 0.464233 4.2:-51925267 MS.gene061316:CDS
TCTTGTTGATTTAGGCTATG+AGG 0.465633 4.2:-51926074 MS.gene061316:CDS
TCTTGCTCGAGGCTTTGATC+AGG 0.465845 4.2:-51925954 MS.gene061316:CDS
ACCCTTGCGCCCAAATCGCC+CGG 0.472926 4.2:+51925729 None:intergenic
AGAACTTGCAATTCCTTGCA+TGG 0.479404 4.2:-51927128 MS.gene061316:CDS
ACTTCGGTGCAGGTTATCTC+CGG 0.480062 4.2:-51927566 MS.gene061316:intron
ATGGGTGGTCGTTTCGATTG+TGG 0.481474 4.2:+51927091 None:intergenic
TCGAGGCTTTGATCAGGATC+AGG 0.484998 4.2:-51925948 MS.gene061316:intron
CTGAATAGCTAACTCTCTAG+TGG 0.485079 4.2:+51927283 None:intergenic
TGCTCTTGAACATGAAGATA+GGG 0.486088 4.2:-51926035 MS.gene061316:CDS
GAACTTGCAATTCCTTGCAT+GGG 0.486931 4.2:-51927127 MS.gene061316:CDS
AAACCGCTTGTTTCGTACTA+GGG 0.495364 4.2:-51927361 MS.gene061316:CDS
ATGCAGTTCTTAATTGTGCC+AGG 0.497085 4.2:+51927043 None:intergenic
GAAGCTCCTTGGAGAGGGAC+AGG 0.497510 4.2:+51927507 None:intergenic
GGATGTGTACGGAGTGTTGC+CGG 0.498474 4.2:+51927547 None:intergenic
TAATCTGATATGTGATGAAA+GGG 0.501009 4.2:-51925558 MS.gene061316:CDS
AAGCTCAGGTTCACGGCCAC+AGG 0.504380 4.2:+51925786 None:intergenic
ATAACCACCTGAGACATAAT+GGG 0.506481 4.2:+51927073 None:intergenic
TGAATAGCTAACTCTCTAGT+GGG 0.508229 4.2:+51927284 None:intergenic
ATACGAGGTTCTTCAAAAGA+TGG 0.508765 4.2:-51927173 MS.gene061316:CDS
TCAATCTTAGCAAGTTCATC+GGG 0.514577 4.2:+51926193 None:intergenic
GAGTCCCTTCAGAAGCTCCT+TGG 0.517455 4.2:+51927496 None:intergenic
CAGAATCAAGCTCAGGTTCA+CGG 0.517842 4.2:+51925779 None:intergenic
TTAATCTGATATGTGATGAA+AGG 0.520394 4.2:-51925559 MS.gene061316:CDS
ACGACCACCCATTATGTCTC+AGG 0.520930 4.2:-51927080 MS.gene061316:CDS
TTCAATCTTAGCAAGTTCAT+CGG 0.523373 4.2:+51926192 None:intergenic
CCTCTCCAAGGAGCTTCTGA+AGG 0.525386 4.2:-51927501 MS.gene061316:CDS
TACGAGGTTCTTCAAAAGAT+GGG 0.527127 4.2:-51927172 MS.gene061316:CDS
TGAACCTGAGCTTGATTCTG+AGG 0.528859 4.2:-51925776 MS.gene061316:CDS
CGTTACAAGGATTGTTGCGA+AGG 0.547449 4.2:-51926287 MS.gene061316:CDS
TCGTTTCGATTGTGGAGTTG+AGG 0.548075 4.2:+51927099 None:intergenic
CTTCAGAAGCTCCTTGGAGA+GGG 0.557207 4.2:+51927502 None:intergenic
TAAACCTCAGAATCAAGCTC+AGG 0.560719 4.2:+51925772 None:intergenic
GTTGAGGTCTTTCCCATGCA+AGG 0.561437 4.2:+51927115 None:intergenic
ATGGTCAAGGAGTTCAAAGA+TGG 0.567169 4.2:-51926007 MS.gene061316:CDS
AATGTTGTGGCGGATGTGTA+CGG 0.570604 4.2:+51927536 None:intergenic
AGCTGATCAAATGTTTGCCA+AGG 0.582447 4.2:-51926969 MS.gene061316:intron
GCCGGGCGATTTGGGCGCAA+GGG 0.582545 4.2:-51925730 MS.gene061316:intron
CATAGACCTGTCCCTCTCCA+AGG 0.586996 4.2:-51927513 MS.gene061316:CDS
ACATGAAGATAGGGACAAGA+TGG 0.587018 4.2:-51926026 MS.gene061316:CDS
GGGCAAATACATAGAGCTTG+AGG 0.596315 4.2:+51927305 None:intergenic
TCAAACGATGTTCTTGCTCG+AGG 0.599572 4.2:-51925965 MS.gene061316:CDS
TTCAAAATAGGAGAGAACAT+CGG 0.600608 4.2:-51926151 MS.gene061316:CDS
GATTCAGAGATGATTCTATG+AGG 0.602154 4.2:-51926656 MS.gene061316:CDS
CCTTCAGAAGCTCCTTGGAG+AGG 0.605973 4.2:+51927501 None:intergenic
AACTCAGCAGTGTAATTCAG+TGG 0.609596 4.2:+51925811 None:intergenic
CTCTCCAAGGAGCTTCTGAA+GGG 0.610324 4.2:-51927500 MS.gene061316:CDS
GGACAGGTCTATGAATGTTG+TGG 0.620529 4.2:+51927523 None:intergenic
GCGATCAAATCCTTATATGG+AGG 0.638313 4.2:+51927407 None:intergenic
TAGCATATACCTCTGTGACA+TGG 0.642965 4.2:+51925495 None:intergenic
AGATAGGGACAAGATGGTCA+AGG 0.653247 4.2:-51926020 MS.gene061316:CDS
CATCCCTAGTACGAAACAAG+CGG 0.659036 4.2:+51927358 None:intergenic
CAGGTCTATGAATGTTGTGG+CGG 0.664552 4.2:+51927526 None:intergenic
TACACTGCTGAGTTCACCTG+TGG 0.672228 4.2:-51925802 MS.gene061316:CDS
ACCACCCATTATGTCTCAGG+TGG 0.688323 4.2:-51927077 MS.gene061316:CDS
ACCACCTGAGACATAATGGG+TGG 0.699071 4.2:+51927076 None:intergenic
GAATAGCTAACTCTCTAGTG+GGG 0.713974 4.2:+51927285 None:intergenic
GTTTGGCAACCATGTCACAG+AGG 0.743084 4.2:-51925504 MS.gene061316:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATTATATATTCAAAAT+AGG - chr4.2:51926729-51926748 MS.gene061316:intron 10.0%
!! ATAAAAAACAATTTGATACA+TGG + chr4.2:51927011-51927030 None:intergenic 15.0%
!! CATTTATTATTATTTGTGCA+TGG - chr4.2:51926367-51926386 MS.gene061316:intron 20.0%
!! TTACTTCCAAGTAAATATAT+AGG - chr4.2:51926094-51926113 MS.gene061316:CDS 20.0%
!!! AATTTTGTATTGGGTATTAT+TGG - chr4.2:51925948-51925967 MS.gene061316:intron 20.0%
!!! ATCTTTTTCTATAGTTCTTT+TGG - chr4.2:51925985-51926004 MS.gene061316:CDS 20.0%
! ATGTAAGAAAAAACACAACT+TGG + chr4.2:51927283-51927302 None:intergenic 25.0%
! CTATGTGAGAATAATAAACA+TGG + chr4.2:51927223-51927242 None:intergenic 25.0%
! GTAAATATATAGGACATACT+AGG - chr4.2:51926104-51926123 MS.gene061316:CDS 25.0%
! GTATGTCCTATATATTTACT+TGG + chr4.2:51926103-51926122 None:intergenic 25.0%
! TAATCTGATATGTGATGAAA+GGG - chr4.2:51927334-51927353 MS.gene061316:CDS 25.0%
! TTAATCTGATATGTGATGAA+AGG - chr4.2:51927333-51927352 MS.gene061316:CDS 25.0%
! TTGATACATGGACAAATAAT+GGG + chr4.2:51926999-51927018 None:intergenic 25.0%
! TTTGATACATGGACAAATAA+TGG + chr4.2:51927000-51927019 None:intergenic 25.0%
! TTTGTTTCATTTGATTGAAC+AGG - chr4.2:51927302-51927321 MS.gene061316:CDS 25.0%
!! TCAAAGTTTGCAAATTTTGA+AGG + chr4.2:51927491-51927510 None:intergenic 25.0%
!! TCATTTTGTTAATTTCACTG+TGG - chr4.2:51927569-51927588 MS.gene061316:intron 25.0%
!!! AAGGAGCATAATGAAATTTT+TGG - chr4.2:51926624-51926643 MS.gene061316:CDS 25.0%
!!! AATGAAATTTTTGGGAAGAA+AGG - chr4.2:51926633-51926652 MS.gene061316:CDS 25.0%
!!! AGGAGCATAATGAAATTTTT+GGG - chr4.2:51926625-51926644 MS.gene061316:CDS 25.0%
!!! ATGAAATTTTTGGGAAGAAA+GGG - chr4.2:51926634-51926653 MS.gene061316:CDS 25.0%
!!! CTTCTTTCTCTTTAAAATCT+TGG - chr4.2:51925277-51925296 MS.gene061316:CDS 25.0%
AAAGTTCAATTCAAACTGTC+AGG - chr4.2:51926276-51926295 MS.gene061316:CDS 30.0%
AAGAATCATTGGCAAACTTA+TGG + chr4.2:51925462-51925481 None:intergenic 30.0%
ACCCAATACAAAATTTACCT+TGG + chr4.2:51925943-51925962 None:intergenic 30.0%
ACTTTATATGCATGTGCAAA+GGG - chr4.2:51927513-51927532 MS.gene061316:CDS 30.0%
ATGGAACGCTGAATATTATA+CGG - chr4.2:51926456-51926475 MS.gene061316:intron 30.0%
ATTATTATTTGTGCATGGAG+TGG - chr4.2:51926372-51926391 MS.gene061316:intron 30.0%
CAAGCATATGAAAAGATAAG+CGG - chr4.2:51926484-51926503 MS.gene061316:intron 30.0%
GATTAACCTAAACAACAAGA+TGG + chr4.2:51927041-51927060 None:intergenic 30.0%
TATTCAAACAATTTCGTCGT+TGG - chr4.2:51927248-51927267 MS.gene061316:intron 30.0%
TCAAAGATTGAAAACCAGTT+TGG - chr4.2:51927371-51927390 MS.gene061316:CDS 30.0%
TTACTGAATTTGTCATGCAT+TGG - chr4.2:51926146-51926165 MS.gene061316:CDS 30.0%
TTCAAAATAGGAGAGAACAT+CGG - chr4.2:51926741-51926760 MS.gene061316:intron 30.0%
TTCAATCTTAGCAAGTTCAT+CGG + chr4.2:51926703-51926722 None:intergenic 30.0%
! AGGTTTGATGCTTTTCTTTT+AGG - chr4.2:51925630-51925649 MS.gene061316:intron 30.0%
! GCCAAGGTAAATTTTGTATT+GGG - chr4.2:51925939-51925958 MS.gene061316:intron 30.0%
! GGTTTGATGCTTTTCTTTTA+GGG - chr4.2:51925631-51925650 MS.gene061316:intron 30.0%
! TGCCAAGGTAAATTTTGTAT+TGG - chr4.2:51925938-51925957 MS.gene061316:intron 30.0%
! TGTTTTGTGTTAGATATACG+AGG - chr4.2:51925704-51925723 MS.gene061316:intron 30.0%
!! ATAGTTCTTTTGGTGTGAAT+CGG - chr4.2:51925995-51926014 MS.gene061316:CDS 30.0%
!! ATGCTACTTGATAATGTACT+TGG - chr4.2:51927414-51927433 MS.gene061316:CDS 30.0%
!! CATAAAGCTCTTGTTGATTT+AGG - chr4.2:51926810-51926829 MS.gene061316:intron 30.0%
!! TCTTGTTGTTTAGGTTAATC+TGG - chr4.2:51927041-51927060 MS.gene061316:CDS 30.0%
!! TGAAATTTTTGGGAAGAAAG+GGG - chr4.2:51926635-51926654 MS.gene061316:CDS 30.0%
!!! ATTCTGAGGTTTATTTTCAC+AGG - chr4.2:51927130-51927149 MS.gene061316:CDS 30.0%
!!! TGTCATTTTGCAACACTAAA+AGG - chr4.2:51926423-51926442 MS.gene061316:intron 30.0%
!!! TTCTGAGGTTTATTTTCACA+GGG - chr4.2:51927131-51927150 MS.gene061316:CDS 30.0%
AAAGATGCTTTCAAGAAAGC+TGG - chr4.2:51927625-51927644 MS.gene061316:intron 35.0%
AATAACCACCTGAGACATAA+TGG + chr4.2:51925823-51925842 None:intergenic 35.0%
AATATTCAGCGTTCCATAAG+TGG + chr4.2:51926453-51926472 None:intergenic 35.0%
ACCGAAGTAAAAAACCCTAA+GGG + chr4.2:51925314-51925333 None:intergenic 35.0%
ACTAAATCTTCTAGCAAGAG+AGG - chr4.2:51926039-51926058 MS.gene061316:CDS 35.0%
AGTAAATCAAAACCATTCCC+TGG + chr4.2:51926131-51926150 None:intergenic 35.0%
ATAACCACCTGAGACATAAT+GGG + chr4.2:51925822-51925841 None:intergenic 35.0%
ATACGAGGTTCTTCAAAAGA+TGG - chr4.2:51925719-51925738 MS.gene061316:intron 35.0%
ATATTCAGCGTTCCATAAGT+GGG + chr4.2:51926452-51926471 None:intergenic 35.0%
ATGCTCTTGAACATGAAGAT+AGG - chr4.2:51926856-51926875 MS.gene061316:intron 35.0%
ATGTTCTACATCATGCTTCA+AGG + chr4.2:51927457-51927476 None:intergenic 35.0%
ATTATGTCTCAGGTGGTTAT+TGG - chr4.2:51925822-51925841 MS.gene061316:CDS 35.0%
CACTTTATATGCATGTGCAA+AGG - chr4.2:51927512-51927531 MS.gene061316:CDS 35.0%
CTGTCAAGAATTTCGTTACA+AGG - chr4.2:51926592-51926611 MS.gene061316:intron 35.0%
GAAAAGATAAGCGGAGATAA+AGG - chr4.2:51926493-51926512 MS.gene061316:intron 35.0%
GATTCAGAGATGATTCTATG+AGG - chr4.2:51926236-51926255 MS.gene061316:CDS 35.0%
GATTCTTGAACCTCCATATA+AGG - chr4.2:51925475-51925494 MS.gene061316:intron 35.0%
GTTAATTTCACTGTGGATGT+AGG - chr4.2:51927576-51927595 MS.gene061316:intron 35.0%
TACGAGGTTCTTCAAAAGAT+GGG - chr4.2:51925720-51925739 MS.gene061316:intron 35.0%
TATGGAGGTTCAAGAATCAT+TGG + chr4.2:51925473-51925492 None:intergenic 35.0%
TCAAAATAGGAGAGAACATC+GGG - chr4.2:51926742-51926761 MS.gene061316:intron 35.0%
TCAATCTTAGCAAGTTCATC+GGG + chr4.2:51926702-51926721 None:intergenic 35.0%
TGAATAGCTAACTCTCTAGT+GGG + chr4.2:51925611-51925630 None:intergenic 35.0%
TGCTCTTGAACATGAAGATA+GGG - chr4.2:51926857-51926876 MS.gene061316:intron 35.0%
TGTGCGATCAAATCCTTATA+TGG + chr4.2:51925491-51925510 None:intergenic 35.0%
! CTTATGGCTTGAATTTTGCT+AGG + chr4.2:51925446-51925465 None:intergenic 35.0%
! GGTTTGGTTGCTAAATATGA+TGG - chr4.2:51926167-51926186 MS.gene061316:CDS 35.0%
! GTTTTCAAAACTGTCTGCTT+AGG - chr4.2:51927188-51927207 MS.gene061316:intron 35.0%
! TACATGGACAAATAATGGGA+AGG + chr4.2:51926995-51927014 None:intergenic 35.0%
!! AAAACTTTGTTGTTGCAGGA+TGG - chr4.2:51926214-51926233 MS.gene061316:CDS 35.0%
!! ATGTTCAAGAGCATCTAGTA+TGG + chr4.2:51926850-51926869 None:intergenic 35.0%
!! CATCTTCCATCTTGTTGTTT+AGG - chr4.2:51927032-51927051 MS.gene061316:CDS 35.0%
!! CTGTAAAACTTTGTTGTTGC+AGG - chr4.2:51926210-51926229 MS.gene061316:CDS 35.0%
!! GAATTTGTCATGCATTGGTT+TGG - chr4.2:51926151-51926170 MS.gene061316:CDS 35.0%
!! TCTTGTTGATTTAGGCTATG+AGG - chr4.2:51926818-51926837 MS.gene061316:intron 35.0%
!! TTTATTTTCACAGGGTTGGT+AGG - chr4.2:51927139-51927158 MS.gene061316:CDS 35.0%
!!! ACCCTTAGGGTTTTTTACTT+CGG - chr4.2:51925310-51925329 MS.gene061316:intron 35.0%
!!! CTTTTCTTTTAGGGCATGTT+TGG - chr4.2:51925640-51925659 MS.gene061316:intron 35.0%
!!! TTATTTTCACAGGGTTGGTA+GGG - chr4.2:51927140-51927159 MS.gene061316:CDS 35.0%
AAAACCGCTTGTTTCGTACT+AGG - chr4.2:51925530-51925549 MS.gene061316:CDS 40.0%
AAACCGCTTGTTTCGTACTA+GGG - chr4.2:51925531-51925550 MS.gene061316:CDS 40.0%
AACTCAGCAGTGTAATTCAG+TGG + chr4.2:51927084-51927103 None:intergenic 40.0%
ACATGAAGATAGGGACAAGA+TGG - chr4.2:51926866-51926885 MS.gene061316:intron 40.0%
AGAACTTGCAATTCCTTGCA+TGG - chr4.2:51925764-51925783 MS.gene061316:CDS 40.0%
AGATAAGCGGAGATAAAGGT+CGG - chr4.2:51926497-51926516 MS.gene061316:intron 40.0%
AGCTGATCAAATGTTTGCCA+AGG - chr4.2:51925923-51925942 MS.gene061316:intron 40.0%
ATAAAGGTCGGAGAGATTGA+AGG - chr4.2:51926509-51926528 MS.gene061316:intron 40.0%
ATAAGTTGTCAATCGAGAGG+CGG - chr4.2:51926400-51926419 MS.gene061316:intron 40.0%
ATATAGGACATACTAGGTCC+AGG - chr4.2:51926110-51926129 MS.gene061316:CDS 40.0%
ATGCAGTTCTTAATTGTGCC+AGG + chr4.2:51925852-51925871 None:intergenic 40.0%
ATGGTCAAGGAGTTCAAAGA+TGG - chr4.2:51926885-51926904 MS.gene061316:intron 40.0%
CACCGAAGTAAAAAACCCTA+AGG + chr4.2:51925315-51925334 None:intergenic 40.0%
CACTAGAGAGTTAGCTATTC+AGG - chr4.2:51925610-51925629 MS.gene061316:intron 40.0%
CTGAATAGCTAACTCTCTAG+TGG + chr4.2:51925612-51925631 None:intergenic 40.0%
GAACTTGCAATTCCTTGCAT+GGG - chr4.2:51925765-51925784 MS.gene061316:CDS 40.0%
GAATAGCTAACTCTCTAGTG+GGG + chr4.2:51925610-51925629 None:intergenic 40.0%
GATTGAAGGTGACTAAGGAT+TGG - chr4.2:51926523-51926542 MS.gene061316:intron 40.0%
GCGATCAAATCCTTATATGG+AGG + chr4.2:51925488-51925507 None:intergenic 40.0%
GCTTGAGGATCATGCAAATT+CGG + chr4.2:51925575-51925594 None:intergenic 40.0%
TAAACCTCAGAATCAAGCTC+AGG + chr4.2:51927123-51927142 None:intergenic 40.0%
TAGCATATACCTCTGTGACA+TGG + chr4.2:51927400-51927419 None:intergenic 40.0%
TATAGGACATACTAGGTCCA+GGG - chr4.2:51926111-51926130 MS.gene061316:CDS 40.0%
TATTTGTGCATGGAGTGGAT+TGG - chr4.2:51926377-51926396 MS.gene061316:intron 40.0%
TGGATAAGTTGTCAATCGAG+AGG - chr4.2:51926397-51926416 MS.gene061316:intron 40.0%
TGGCGAGAAATTAACCCTTA+GGG - chr4.2:51925297-51925316 MS.gene061316:CDS 40.0%
TTGGCGAGAAATTAACCCTT+AGG - chr4.2:51925296-51925315 MS.gene061316:CDS 40.0%
!! GAGGTTTATTTTCACAGGGT+TGG - chr4.2:51927135-51927154 MS.gene061316:CDS 40.0%
AGATAGGGACAAGATGGTCA+AGG - chr4.2:51926872-51926891 MS.gene061316:intron 45.0%
ATCAGGATCAGGTGCTCATT+TGG - chr4.2:51926955-51926974 MS.gene061316:intron 45.0%
CAGAATCAAGCTCAGGTTCA+CGG + chr4.2:51927116-51927135 None:intergenic 45.0%
CAGGTCTATGAATGTTGTGG+CGG + chr4.2:51925369-51925388 None:intergenic 45.0%
CATCCCTAGTACGAAACAAG+CGG + chr4.2:51925537-51925556 None:intergenic 45.0%
CGTTACAAGGATTGTTGCGA+AGG - chr4.2:51926605-51926624 MS.gene061316:intron 45.0%
GGACAGGTCTATGAATGTTG+TGG + chr4.2:51925372-51925391 None:intergenic 45.0%
GGAGAGATTGAAGGTGACTA+AGG - chr4.2:51926518-51926537 MS.gene061316:intron 45.0%
GGGCAAATACATAGAGCTTG+AGG + chr4.2:51925590-51925609 None:intergenic 45.0%
TCAAACGATGTTCTTGCTCG+AGG - chr4.2:51926927-51926946 MS.gene061316:intron 45.0%
TCGTTTCGATTGTGGAGTTG+AGG + chr4.2:51925796-51925815 None:intergenic 45.0%
TGAACCTGAGCTTGATTCTG+AGG - chr4.2:51927116-51927135 MS.gene061316:CDS 45.0%
TTGATCGCACAAGCACCTAA+TGG - chr4.2:51925500-51925519 MS.gene061316:intron 45.0%
!! AATGTTGTGGCGGATGTGTA+CGG + chr4.2:51925359-51925378 None:intergenic 45.0%
!! GAAGGGACTCTTTTTGGAGA+TGG - chr4.2:51925409-51925428 MS.gene061316:intron 45.0%
!! GCTTCTGAAGGGACTCTTTT+TGG - chr4.2:51925403-51925422 MS.gene061316:intron 45.0%
!!! AGGGTTTTTTACTTCGGTGC+AGG - chr4.2:51925316-51925335 MS.gene061316:intron 45.0%
!!! GGATTGGCTTCTGTTTTTGC+AGG - chr4.2:51926539-51926558 MS.gene061316:intron 45.0%
ACCACCCATTATGTCTCAGG+TGG - chr4.2:51925815-51925834 MS.gene061316:CDS 50.0%
ACGACCACCCATTATGTCTC+AGG - chr4.2:51925812-51925831 MS.gene061316:CDS 50.0%
ACTAAAAGGCCGCCCACTTA+TGG - chr4.2:51926437-51926456 MS.gene061316:intron 50.0%
ATGGGTGGTCGTTTCGATTG+TGG + chr4.2:51925804-51925823 None:intergenic 50.0%
CTAGCAAGAGAGGACCTCTA+CGG - chr4.2:51926049-51926068 MS.gene061316:CDS 50.0%
CTCTCCAAGGAGCTTCTGAA+GGG - chr4.2:51925392-51925411 MS.gene061316:intron 50.0%
CTGTGACATGGTTGCCAAAC+TGG + chr4.2:51927388-51927407 None:intergenic 50.0%
CTTCAGAAGCTCCTTGGAGA+GGG + chr4.2:51925393-51925412 None:intergenic 50.0%
GACATACTAGGTCCAGGGAA+TGG - chr4.2:51926116-51926135 MS.gene061316:CDS 50.0%
GCACAAGCACCTAATGGTTC+TGG - chr4.2:51925506-51925525 MS.gene061316:CDS 50.0%
GTTGAGGTCTTTCCCATGCA+AGG + chr4.2:51925780-51925799 None:intergenic 50.0%
GTTTGGCAACCATGTCACAG+AGG - chr4.2:51927388-51927407 MS.gene061316:CDS 50.0%
TACACTGCTGAGTTCACCTG+TGG - chr4.2:51927090-51927109 MS.gene061316:CDS 50.0%
TAGCAAGAGAGGACCTCTAC+GGG - chr4.2:51926050-51926069 MS.gene061316:CDS 50.0%
TTCAGCGTTCCATAAGTGGG+CGG + chr4.2:51926449-51926468 None:intergenic 50.0%
TTTAGTCTCTACGAGCGCAG+AGG + chr4.2:51926025-51926044 None:intergenic 50.0%
! ACCACCTGAGACATAATGGG+TGG + chr4.2:51925819-51925838 None:intergenic 50.0%
! ACTTCGGTGCAGGTTATCTC+CGG - chr4.2:51925326-51925345 MS.gene061316:intron 50.0%
! GCGGTTTTGCCAGAACCATT+AGG + chr4.2:51925518-51925537 None:intergenic 50.0%
! TATAGCTTTGCCACCCGTAG+AGG + chr4.2:51926066-51926085 None:intergenic 50.0%
! TCGAGGCTTTGATCAGGATC+AGG - chr4.2:51926944-51926963 MS.gene061316:intron 50.0%
! TCTTGCTCGAGGCTTTGATC+AGG - chr4.2:51926938-51926957 MS.gene061316:intron 50.0%
CAGGTGGTTATTGGCACTCC+TGG - chr4.2:51925831-51925850 MS.gene061316:CDS 55.0%
CATAGACCTGTCCCTCTCCA+AGG - chr4.2:51925379-51925398 MS.gene061316:intron 55.0%
CCTCTCCAAGGAGCTTCTGA+AGG - chr4.2:51925391-51925410 MS.gene061316:intron 55.0%
CCTTCAGAAGCTCCTTGGAG+AGG + chr4.2:51925394-51925413 None:intergenic 55.0%
GAGTCCCTTCAGAAGCTCCT+TGG + chr4.2:51925399-51925418 None:intergenic 55.0%
!! GGATGTGTACGGAGTGTTGC+CGG + chr4.2:51925348-51925367 None:intergenic 55.0%
!! TTTCACAGGGTTGGTAGGGC+CGG - chr4.2:51927144-51927163 MS.gene061316:CDS 55.0%
AAGCTCAGGTTCACGGCCAC+AGG + chr4.2:51927109-51927128 None:intergenic 60.0%
CAAGAGAGGACCTCTACGGG+TGG - chr4.2:51926053-51926072 MS.gene061316:CDS 60.0%
GAAGCTCCTTGGAGAGGGAC+AGG + chr4.2:51925388-51925407 None:intergenic 60.0%
TTGGTAGGGCCGGGCGATTT+GGG - chr4.2:51927154-51927173 MS.gene061316:CDS 60.0%
!! TTCACAGGGTTGGTAGGGCC+GGG - chr4.2:51927145-51927164 MS.gene061316:CDS 60.0%
ACCCTTGCGCCCAAATCGCC+CGG + chr4.2:51927166-51927185 None:intergenic 65.0%
! GTTGGTAGGGCCGGGCGATT+TGG - chr4.2:51927153-51927172 MS.gene061316:CDS 65.0%
! GCCGGGCGATTTGGGCGCAA+GGG - chr4.2:51927162-51927181 MS.gene061316:CDS 70.0%
! GGCCGGGCGATTTGGGCGCA+AGG - chr4.2:51927161-51927180 MS.gene061316:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 51925254 51927660 51925254 ID=MS.gene061316
chr4.2 mRNA 51925254 51927660 51925254 ID=MS.gene061316.t1;Parent=MS.gene061316
chr4.2 exon 51927658 51927660 51927658 ID=MS.gene061316.t1.exon1;Parent=MS.gene061316.t1
chr4.2 CDS 51927658 51927660 51927658 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51927283 51927576 51927283 ID=MS.gene061316.t1.exon2;Parent=MS.gene061316.t1
chr4.2 CDS 51927283 51927576 51927283 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51926970 51927197 51926970 ID=MS.gene061316.t1.exon3;Parent=MS.gene061316.t1
chr4.2 CDS 51926970 51927197 51926970 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51926617 51926682 51926617 ID=MS.gene061316.t1.exon4;Parent=MS.gene061316.t1
chr4.2 CDS 51926617 51926682 51926617 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51925949 51926353 51925949 ID=MS.gene061316.t1.exon5;Parent=MS.gene061316.t1
chr4.2 CDS 51925949 51926353 51925949 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51925731 51925860 51925731 ID=MS.gene061316.t1.exon6;Parent=MS.gene061316.t1
chr4.2 CDS 51925731 51925860 51925731 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51925505 51925590 51925505 ID=MS.gene061316.t1.exon7;Parent=MS.gene061316.t1
chr4.2 CDS 51925505 51925590 51925505 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
chr4.2 exon 51925254 51925316 51925254 ID=MS.gene061316.t1.exon8;Parent=MS.gene061316.t1
chr4.2 CDS 51925254 51925316 51925254 ID=cds.MS.gene061316.t1;Parent=MS.gene061316.t1
Gene Sequence

>MS.gene061316

ATGGTTCGACACTGATCTTCTTTCTTCTTTCTCTTTAAAATCTTGGCGAGAAATTAACCCTTAGGGTTTTTTACTTCGGTGCAGGTTATCTCCGGCAACACTCCGTACACATCCGCCACAACATTCATAGACCTGTCCCTCTCCAAGGAGCTTCTGAAGGGACTCTTTTTGGAGATGGAGTTTCGAAAGCCTAGCAAAATTCAAGCCATAAGTTTGCCAATGATTCTTGAACCTCCATATAAGGATTTGATCGCACAAGCACCTAATGGTTCTGGCAAAACCGCTTGTTTCGTACTAGGGATGCTTAGTCGTGTTGATCCGAATTTGCATGATCCTCAAGCTCTATGTATTTGCCCCACTAGAGAGTTAGCTATTCAGGTTTGATGCTTTTCTTTTAGGGCATGTTTGGATAAATAAACTAGTTAATTATTTGATTTTTGTTTATTGTTGTGTTTTGTGTTAGATATACGAGGTTCTTCAAAAGATGGGAAAGTACACACGCATTATTTCAGAACTTGCAATTCCTTGCATGGGAAAGACCTCAACTCCACAATCGAAACGACCACCCATTATGTCTCAGGTGGTTATTGGCACTCCTGGCACAATTAAGAACTGCATGATTTATAAAAAACTCGAGATGACGAGATTGAAGATTCTTGTATTTGATGAAGCTGATCAAATGTTTGCCAAGGTAAATTTTGTATTGGGTATTATTGGAGATATAGTTAGATATCTTTTTCTATAGTTCTTTTGGTGTGAATCGGATATCCTCTGCGCTCGTAGAGACTAAATCTTCTAGCAAGAGAGGACCTCTACGGGTGGCAAAGCTATAACTAGTAATTACTTCCAAGTAAATATATAGGACATACTAGGTCCAGGGAATGGTTTTGATTTACTGAATTTGTCATGCATTGGTTTGGTTGCTAAATATGATGGTTTCGTAATTTACTTAATAACTGTAAAACTTTGTTGTTGCAGGATGGATTCAGAGATGATTCTATGAGGATAATAAAAGAAACACAAAAGTTCAATTCAAACTGTCAGGTTTTTTGATTCTCTTGCTTAATGCACTTGCAAATATTAAATATTTTTCTGCTTGATGTTATTGTGTTACATTTATTATTATTTGTGCATGGAGTGGATTGGATAAGTTGTCAATCGAGAGGCGGTGTCATTTTGCAACACTAAAAGGCCGCCCACTTATGGAACGCTGAATATTATACGGAGATACAAGCATATGAAAAGATAAGCGGAGATAAAGGTCGGAGAGATTGAAGGTGACTAAGGATTGGCTTCTGTTTTTGCAGGTTCTCTTTTTTTCTGCTACATTAAATGACGCTGTCAAGAATTTCGTTACAAGGATTGTTGCGAAGGAGCATAATGAAATTTTTGGGAAGAAAGGGGAGCTATCTTTAGAAGCAGTGAAGCAGTACAAAGTGCATTGTCCCGATGAACTTGCTAAGATTGAAGTGATTAAAGATTATATATTCAAAATAGGAGAGAACATCGGGAAAACAATTATCTTTGTGCGCACTACAAATAGTGCAAAAGTGTTGCATAAAGCTCTTGTTGATTTAGGCTATGAGGTTTGTTCCATACTAGATGCTCTTGAACATGAAGATAGGGACAAGATGGTCAAGGAGTTCAAAGATGGTTCGACTCGTGTTCTTATATCAAACGATGTTCTTGCTCGAGGCTTTGATCAGGATCAGGTGCTCATTTGGTTTCTCTATCTATGCCTTCCCATTATTTGTCCATGTATCAAATTGTTTTTTATGCATCTTCCATCTTGTTGTTTAGGTTAATCTGGTTATCAACTATGATCTTCCACTGAATTACACTGCTGAGTTCACCTGTGGCCGTGAACCTGAGCTTGATTCTGAGGTTTATTTTCACAGGGTTGGTAGGGCCGGGCGATTTGGGCGCAAGGGTAAGTTTTCAAAACTGTCTGCTTAGGTGAAATTTACCATGTTTATTATTCTCACATAGTATAGTATTCAAACAATTTCGTCGTTGGTGAAAAATACCAAGTTGTGTTTTTTCTTACATTTGTTTCATTTGATTGAACAGGTGCTGTATTTAATCTGATATGTGATGAAAGGGATGAGAATTTAATGTCAAAGATTGAAAACCAGTTTGGCAACCATGTCACAGAGGTATATGCTACTTGATAATGTACTTGGATTATGATTATGATGATCCTTGAAGCATGATGTAGAACATTAAAGATTAAACCTTCAAAATTTGCAAACTTTGAACACTTTATATGCATGTGCAAAGGGTTATGACTTGTGACAATATGAATCTTACATAAATCATTTTGTTAATTTCACTGTGGATGTAGGTGCGACAAAAATGTGTTGAAGACTATAAAGATGCTTTCAAGAAAGCTGGTTTACTTTACTGA
Protein sequence

>MS.gene061316.t1

MVISGNTPYTSATTFIDLSLSKELLKGLFLEMEFRKPSKIQAISLPMILEPPYKDLIAQAPNGSGKTACFVLGMLSRVDPNLHDPQALCICPTRELAIQIYEVLQKMGKYTRIISELAIPCMGKTSTPQSKRPPIMSQVVIGTPGTIKNCMIYKKLEMTRLKILVFDEADQMFAKDGFRDDSMRIIKETQKFNSNCQVLFFSATLNDAVKNFVTRIVAKEHNEIFGKKGELSLEAVKQYKVHCPDELAKIEVIKDYIFKIGENIGKTIIFVRTTNSAKVLHKALVDLGYEVCSILDALEHEDRDKMVKEFKDGSTRVLISNDVLARGFDQDQVNLVINYDLPLNYTAEFTCGREPELDSEVYFHRVGRAGRFGRKGAVFNLICDERDENLMSKIENQFGNHVTEVRQKCVEDYKDAFKKAGLLY