Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062775.t1 | XP_003589223.1 | 95.2 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 5.60E-111 | 410.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062775.t1 | G7I4F7 | 95.2 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 4.1e-111 | 410.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene062775 | 0.816972 | 4.20E-52 | -1.69E-46 |
| MS.gene050680 | MS.gene062775 | 0.812825 | 3.49E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene062775 | 0.806307 | 8.76E-50 | -1.69E-46 |
| MS.gene050787 | MS.gene062775 | 0.839292 | 1.73E-57 | -1.69E-46 |
| MS.gene050844 | MS.gene062775 | 0.809296 | 2.03E-50 | -1.69E-46 |
| MS.gene051207 | MS.gene062775 | 0.809483 | 1.85E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene062775 | 0.843695 | 1.20E-58 | -1.69E-46 |
| MS.gene052190 | MS.gene062775 | 0.820089 | 8.24E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene062775 | 0.807581 | 4.71E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene062775 | 0.800671 | 1.29E-48 | -1.69E-46 |
| MS.gene053166 | MS.gene062775 | 0.834742 | 2.51E-56 | -1.69E-46 |
| MS.gene054093 | MS.gene062775 | 0.800272 | 1.56E-48 | -1.69E-46 |
| MS.gene054291 | MS.gene062775 | -0.818285 | 2.12E-52 | -1.69E-46 |
| MS.gene054467 | MS.gene062775 | 0.841981 | 3.42E-58 | -1.69E-46 |
| MS.gene054468 | MS.gene062775 | 0.844518 | 7.19E-59 | -1.69E-46 |
| MS.gene054559 | MS.gene062775 | 0.822701 | 2.06E-53 | -1.69E-46 |
| MS.gene05468 | MS.gene062775 | 0.818489 | 1.91E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene062775 | 0.857286 | 1.81E-62 | -1.69E-46 |
| MS.gene055357 | MS.gene062775 | 0.828306 | 9.67E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene062775 | 0.858455 | 8.14E-63 | -1.69E-46 |
| MS.gene055701 | MS.gene062775 | 0.818068 | 2.38E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene062775 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene062775 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene062775 | 0.810131 | 1.34E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene062775 | 0.811071 | 8.41E-51 | -1.69E-46 |
| MS.gene059789 | MS.gene062775 | 0.800585 | 1.34E-48 | -1.69E-46 |
| MS.gene060137 | MS.gene062775 | -0.80894 | 2.42E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene062775 | 0.811609 | 6.43E-51 | -1.69E-46 |
| MS.gene060683 | MS.gene062775 | 0.817246 | 3.64E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene062775 | 0.821956 | 3.06E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene062775 | -0.81327 | 2.79E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062775.t1 | MTR_1g019800 | 95.192 | 208 | 10 | 0 | 1 | 208 | 1 | 208 | 7.13e-148 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062775.t1 | AT1G47410 | 38.500 | 200 | 87 | 6 | 21 | 208 | 9 | 184 | 1.98e-32 | 116 |
| MS.gene062775.t1 | AT1G47410 | 38.500 | 200 | 87 | 6 | 21 | 208 | 9 | 184 | 1.98e-32 | 116 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCATCAAGATCACTAAT+TGG | 0.271099 | 1.4:+3466608 | None:intergenic |
| ATTATGTGTTACTGGGAAAA+TGG | 0.272982 | 1.4:-3466327 | MS.gene062775:CDS |
| TTGGAAGCGTTCTATAATTC+AGG | 0.285690 | 1.4:-3466132 | MS.gene062775:CDS |
| ACAAGAGAATTATGTGTTAC+TGG | 0.321124 | 1.4:-3466335 | MS.gene062775:CDS |
| TTACCTCCCTCCTTTGCCTT+TGG | 0.326286 | 1.4:+3466302 | None:intergenic |
| GTGTATTCTTCTAGTGCTAA+TGG | 0.339241 | 1.4:-3466227 | MS.gene062775:CDS |
| TGCAAAGCATTTGCATCTCT+TGG | 0.342800 | 1.4:+3466715 | None:intergenic |
| CATTGTGAAGGATGGCCTTT+CGG | 0.363378 | 1.4:-3466101 | MS.gene062775:CDS |
| ACACATAATTCTCTTGTTGC+AGG | 0.374763 | 1.4:+3466341 | None:intergenic |
| TGTATTCTTCTAGTGCTAAT+GGG | 0.374850 | 1.4:-3466226 | MS.gene062775:CDS |
| TTGCATCTCTTGGAATGGTT+TGG | 0.387372 | 1.4:+3466725 | None:intergenic |
| CATATAAATTCAAGTAACTT+TGG | 0.429792 | 1.4:+3465995 | None:intergenic |
| TAGTGATCTTGATGAAGAAC+AGG | 0.434961 | 1.4:-3466603 | MS.gene062775:CDS |
| ATTCAAGTAACTTTGGTACT+TGG | 0.457278 | 1.4:+3466002 | None:intergenic |
| ATGCTTTGCAGCTGCTCTGA+AGG | 0.476673 | 1.4:-3466702 | MS.gene062775:CDS |
| TAATTGGAAACTCCTCTTCA+AGG | 0.478540 | 1.4:+3466624 | None:intergenic |
| GGTTTGGTTGCTCCTCCTCA+TGG | 0.490726 | 1.4:+3466741 | None:intergenic |
| GGCGGTTAGAAGCCGAGCTA+TGG | 0.496315 | 1.4:-3466420 | MS.gene062775:CDS |
| AAACTCTCTCTCAGTAGGCT+TGG | 0.513974 | 1.4:+3466380 | None:intergenic |
| CTAAGTCACCATCTGAATCA+TGG | 0.518553 | 1.4:-3466043 | MS.gene062775:CDS |
| AGCATTTGCATCTCTTGGAA+TGG | 0.526952 | 1.4:+3466720 | None:intergenic |
| CAAGAGAATTATGTGTTACT+GGG | 0.534810 | 1.4:-3466334 | MS.gene062775:CDS |
| TTCGACCAAAAGTTTGACAC+TGG | 0.545482 | 1.4:+3466669 | None:intergenic |
| TCTTCTAGTGCTAATGGGGA+AGG | 0.547226 | 1.4:-3466221 | MS.gene062775:CDS |
| TCAAGCTTGAACATCTTGAA+AGG | 0.551732 | 1.4:+3466177 | None:intergenic |
| AATGGAACCAAAGGCAAAGG+AGG | 0.558037 | 1.4:-3466309 | MS.gene062775:CDS |
| TACTGGGAAAATGGAACCAA+AGG | 0.569555 | 1.4:-3466318 | MS.gene062775:CDS |
| TTCGACTACAAGCCTTGAAG+AGG | 0.572674 | 1.4:-3466636 | MS.gene062775:CDS |
| CGATACAACCATGATTCAGA+TGG | 0.575454 | 1.4:+3466035 | None:intergenic |
| CATGATTCAGATGGTGACTT+AGG | 0.584417 | 1.4:+3466044 | None:intergenic |
| TCGACCAAAAGTTTGACACT+GGG | 0.608137 | 1.4:+3466670 | None:intergenic |
| GTATTCTTCTAGTGCTAATG+GGG | 0.608962 | 1.4:-3466225 | MS.gene062775:CDS |
| GATGGTGACTTAGGCAGTGG+TGG | 0.623202 | 1.4:+3466053 | None:intergenic |
| GAAAATGGAACCAAAGGCAA+AGG | 0.636908 | 1.4:-3466312 | MS.gene062775:CDS |
| ATGGAACCAAAGGCAAAGGA+GGG | 0.641016 | 1.4:-3466308 | MS.gene062775:CDS |
| GCTTTACGACAGAGACCGAA+AGG | 0.645910 | 1.4:+3466086 | None:intergenic |
| GAGGGAGGTAATGAAGAACA+AGG | 0.652290 | 1.4:-3466290 | MS.gene062775:CDS |
| TCAGATGGTGACTTAGGCAG+TGG | 0.663320 | 1.4:+3466050 | None:intergenic |
| GAACCAAAGGCAAAGGAGGG+AGG | 0.663364 | 1.4:-3466305 | MS.gene062775:CDS |
| TCTTCAGGTGATTGTCTCGG+CGG | 0.664433 | 1.4:-3466438 | MS.gene062775:intron |
| TGTTGCAGGAGAATATACCC+AGG | 0.714302 | 1.4:+3466355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAGTTTTTCTTTGATTTTA+AGG | + | chr1.4:3466213-3466232 | None:intergenic | 15.0% |
| !!! | AATTAGTTATTGTTCTTTTG+TGG | - | chr1.4:3466295-3466314 | MS.gene062775:CDS | 20.0% |
| !!! | ATTTTGATTTGTCAGAATAT+TGG | - | chr1.4:3466608-3466627 | MS.gene062775:CDS | 20.0% |
| !!! | TTCTTTGATTTTAAGGTTGA+AGG | + | chr1.4:3466206-3466225 | None:intergenic | 25.0% |
| ACAAGAGAATTATGTGTTAC+TGG | - | chr1.4:3466424-3466443 | MS.gene062775:CDS | 30.0% | |
| TCTTCATCAAGATCACTAAT+TGG | + | chr1.4:3466154-3466173 | None:intergenic | 30.0% | |
| TGTATTCTTCTAGTGCTAAT+GGG | - | chr1.4:3466533-3466552 | MS.gene062775:intron | 30.0% | |
| ! | ATTATGTGTTACTGGGAAAA+TGG | - | chr1.4:3466432-3466451 | MS.gene062775:CDS | 30.0% |
| ! | CAAGAGAATTATGTGTTACT+GGG | - | chr1.4:3466425-3466444 | MS.gene062775:CDS | 30.0% |
| ! | TGAAAGAGAAGAGTTTTTGT+CGG | - | chr1.4:3466492-3466511 | MS.gene062775:intron | 30.0% |
| ACACATAATTCTCTTGTTGC+AGG | + | chr1.4:3466421-3466440 | None:intergenic | 35.0% | |
| GTATTCTTCTAGTGCTAATG+GGG | - | chr1.4:3466534-3466553 | MS.gene062775:intron | 35.0% | |
| GTGTATTCTTCTAGTGCTAA+TGG | - | chr1.4:3466532-3466551 | MS.gene062775:intron | 35.0% | |
| TAATTGGAAACTCCTCTTCA+AGG | + | chr1.4:3466138-3466157 | None:intergenic | 35.0% | |
| TAGTGATCTTGATGAAGAAC+AGG | - | chr1.4:3466156-3466175 | MS.gene062775:CDS | 35.0% | |
| TCAAGCTTGAACATCTTGAA+AGG | + | chr1.4:3466585-3466604 | None:intergenic | 35.0% | |
| ! | GTTCTTTTGTGGTTTTCTTC+AGG | - | chr1.4:3466306-3466325 | MS.gene062775:CDS | 35.0% |
| ! | TTTTCCCAGTGTCAAACTTT+TGG | - | chr1.4:3466085-3466104 | MS.gene062775:CDS | 35.0% |
| ! | TTTTCTTCAGGTGATTGTCT+CGG | - | chr1.4:3466318-3466337 | MS.gene062775:CDS | 35.0% |
| !! | AGTTTTGTCATTGTGAAGGA+TGG | - | chr1.4:3466650-3466669 | MS.gene062775:CDS | 35.0% |
| !! | TCTTTTGTTTCTCCATAGCT+CGG | + | chr1.4:3466354-3466373 | None:intergenic | 35.0% |
| !! | TTGGAAGCGTTCTATAATTC+AGG | - | chr1.4:3466627-3466646 | MS.gene062775:CDS | 35.0% |
| AGCATTTGCATCTCTTGGAA+TGG | + | chr1.4:3466042-3466061 | None:intergenic | 40.0% | |
| AGGAAAAACTCTCTCTCAGT+AGG | + | chr1.4:3466387-3466406 | None:intergenic | 40.0% | |
| CATGATTCAGATGGTGACTT+AGG | + | chr1.4:3466718-3466737 | None:intergenic | 40.0% | |
| CGATACAACCATGATTCAGA+TGG | + | chr1.4:3466727-3466746 | None:intergenic | 40.0% | |
| CTAAGTCACCATCTGAATCA+TGG | - | chr1.4:3466716-3466735 | MS.gene062775:CDS | 40.0% | |
| GAAAATGGAACCAAAGGCAA+AGG | - | chr1.4:3466447-3466466 | MS.gene062775:intron | 40.0% | |
| TACTGGGAAAATGGAACCAA+AGG | - | chr1.4:3466441-3466460 | MS.gene062775:intron | 40.0% | |
| TCGACCAAAAGTTTGACACT+GGG | + | chr1.4:3466092-3466111 | None:intergenic | 40.0% | |
| TTCGACCAAAAGTTTGACAC+TGG | + | chr1.4:3466093-3466112 | None:intergenic | 40.0% | |
| TTGCATCTCTTGGAATGGTT+TGG | + | chr1.4:3466037-3466056 | None:intergenic | 40.0% | |
| ! | AAACTTTTGGTCGAAGTCGA+AGG | - | chr1.4:3466098-3466117 | MS.gene062775:CDS | 40.0% |
| ! | ACTGAGAGAGAGTTTTTCCT+GGG | - | chr1.4:3466387-3466406 | MS.gene062775:CDS | 40.0% |
| ! | TACTGAGAGAGAGTTTTTCC+TGG | - | chr1.4:3466386-3466405 | MS.gene062775:CDS | 40.0% |
| ! | TGCAAAGCATTTGCATCTCT+TGG | + | chr1.4:3466047-3466066 | None:intergenic | 40.0% |
| !! | CAGGAGTTTTGTCATTGTGA+AGG | - | chr1.4:3466646-3466665 | MS.gene062775:CDS | 40.0% |
| AAACTCTCTCTCAGTAGGCT+TGG | + | chr1.4:3466382-3466401 | None:intergenic | 45.0% | |
| AATGGAACCAAAGGCAAAGG+AGG | - | chr1.4:3466450-3466469 | MS.gene062775:intron | 45.0% | |
| ATGGAACCAAAGGCAAAGGA+GGG | - | chr1.4:3466451-3466470 | MS.gene062775:intron | 45.0% | |
| CATTGTGAAGGATGGCCTTT+CGG | - | chr1.4:3466658-3466677 | MS.gene062775:CDS | 45.0% | |
| GAGGGAGGTAATGAAGAACA+AGG | - | chr1.4:3466469-3466488 | MS.gene062775:intron | 45.0% | |
| TCTTCTAGTGCTAATGGGGA+AGG | - | chr1.4:3466538-3466557 | MS.gene062775:intron | 45.0% | |
| TGTTGCAGGAGAATATACCC+AGG | + | chr1.4:3466407-3466426 | None:intergenic | 45.0% | |
| TTCGACTACAAGCCTTGAAG+AGG | - | chr1.4:3466123-3466142 | MS.gene062775:CDS | 45.0% | |
| ATGCTTTGCAGCTGCTCTGA+AGG | - | chr1.4:3466057-3466076 | MS.gene062775:CDS | 50.0% | |
| GCTTTACGACAGAGACCGAA+AGG | + | chr1.4:3466676-3466695 | None:intergenic | 50.0% | |
| TCAGATGGTGACTTAGGCAG+TGG | + | chr1.4:3466712-3466731 | None:intergenic | 50.0% | |
| TCTTCAGGTGATTGTCTCGG+CGG | - | chr1.4:3466321-3466340 | MS.gene062775:CDS | 50.0% | |
| TTACCTCCCTCCTTTGCCTT+TGG | + | chr1.4:3466460-3466479 | None:intergenic | 50.0% | |
| ! | GGGTGTTTTCTACCATGAGG+AGG | - | chr1.4:3466006-3466025 | MS.gene062775:CDS | 50.0% |
| GAACCAAAGGCAAAGGAGGG+AGG | - | chr1.4:3466454-3466473 | MS.gene062775:intron | 55.0% | |
| GATGGTGACTTAGGCAGTGG+TGG | + | chr1.4:3466709-3466728 | None:intergenic | 55.0% | |
| ! | GGTTTGGTTGCTCCTCCTCA+TGG | + | chr1.4:3466021-3466040 | None:intergenic | 55.0% |
| GGCGGTTAGAAGCCGAGCTA+TGG | - | chr1.4:3466339-3466358 | MS.gene062775:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 3466004 | 3466777 | 3466004 | ID=MS.gene062775 |
| chr1.4 | mRNA | 3466004 | 3466777 | 3466004 | ID=MS.gene062775.t1;Parent=MS.gene062775 |
| chr1.4 | exon | 3466601 | 3466777 | 3466601 | ID=MS.gene062775.t1.exon1;Parent=MS.gene062775.t1 |
| chr1.4 | CDS | 3466601 | 3466777 | 3466601 | ID=cds.MS.gene062775.t1;Parent=MS.gene062775.t1 |
| chr1.4 | exon | 3466004 | 3466453 | 3466004 | ID=MS.gene062775.t1.exon2;Parent=MS.gene062775.t1 |
| chr1.4 | CDS | 3466004 | 3466453 | 3466004 | ID=cds.MS.gene062775.t1;Parent=MS.gene062775.t1 |
| Gene Sequence |
| Protein sequence |
