Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066875.t1 | RHN40643.1 | 93.6 | 690 | 44 | 0 | 3 | 692 | 5 | 694 | 5.90E-290 | 1006.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066875.t1 | Q9SA32 | 51.3 | 380 | 151 | 3 | 313 | 692 | 6 | 351 | 4.0e-93 | 344.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066875.t1 | A0A396GJC6 | 93.6 | 690 | 44 | 0 | 3 | 692 | 5 | 694 | 4.3e-290 | 1006.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049251 | MS.gene066875 | 0.820315 | 7.32E-53 | -1.69E-46 |
| MS.gene049596 | MS.gene066875 | 0.853137 | 2.91E-61 | -1.69E-46 |
| MS.gene050297 | MS.gene066875 | 0.84183 | 3.74E-58 | -1.69E-46 |
| MS.gene05124 | MS.gene066875 | 0.858151 | 1.00E-62 | -1.69E-46 |
| MS.gene051914 | MS.gene066875 | 0.806186 | 9.29E-50 | -1.69E-46 |
| MS.gene052367 | MS.gene066875 | 0.816574 | 5.16E-52 | -1.69E-46 |
| MS.gene053173 | MS.gene066875 | 0.810727 | 9.98E-51 | -1.69E-46 |
| MS.gene053348 | MS.gene066875 | 0.80059 | 1.34E-48 | -1.69E-46 |
| MS.gene053416 | MS.gene066875 | 0.811427 | 7.04E-51 | -1.69E-46 |
| MS.gene053417 | MS.gene066875 | 0.805964 | 1.04E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene066875 | 0.80511 | 1.56E-49 | -1.69E-46 |
| MS.gene054593 | MS.gene066875 | 0.800789 | 1.22E-48 | -1.69E-46 |
| MS.gene054633 | MS.gene066875 | 0.810748 | 9.88E-51 | -1.69E-46 |
| MS.gene054643 | MS.gene066875 | 0.845579 | 3.72E-59 | -1.69E-46 |
| MS.gene056041 | MS.gene066875 | 0.822503 | 2.29E-53 | -1.69E-46 |
| MS.gene05640 | MS.gene066875 | 0.814304 | 1.65E-51 | -1.69E-46 |
| MS.gene058818 | MS.gene066875 | 0.800255 | 1.57E-48 | -1.69E-46 |
| MS.gene058844 | MS.gene066875 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene060565 | MS.gene066875 | 0.806134 | 9.53E-50 | -1.69E-46 |
| MS.gene06093 | MS.gene066875 | 0.815745 | 7.90E-52 | -1.69E-46 |
| MS.gene061099 | MS.gene066875 | 0.819002 | 1.46E-52 | -1.69E-46 |
| MS.gene061260 | MS.gene066875 | 0.851719 | 7.39E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene67725 | MS.gene066875 | PPI |
| MS.gene00063 | MS.gene066875 | PPI |
| MS.gene78156 | MS.gene066875 | PPI |
| MS.gene55900 | MS.gene066875 | PPI |
| MS.gene67743 | MS.gene066875 | PPI |
| MS.gene009372 | MS.gene066875 | PPI |
| MS.gene28739 | MS.gene066875 | PPI |
| MS.gene028366 | MS.gene066875 | PPI |
| MS.gene071261 | MS.gene066875 | PPI |
| MS.gene76034 | MS.gene066875 | PPI |
| MS.gene36424 | MS.gene066875 | PPI |
| MS.gene35086 | MS.gene066875 | PPI |
| MS.gene066551 | MS.gene066875 | PPI |
| MS.gene00146 | MS.gene066875 | PPI |
| MS.gene51515 | MS.gene066875 | PPI |
| MS.gene55980 | MS.gene066875 | PPI |
| MS.gene67884 | MS.gene066875 | PPI |
| MS.gene074258 | MS.gene066875 | PPI |
| MS.gene058245 | MS.gene066875 | PPI |
| MS.gene045765 | MS.gene066875 | PPI |
| MS.gene066552 | MS.gene066875 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066875.t1 | MTR_5g023790 | 47.679 | 237 | 118 | 2 | 462 | 692 | 144 | 380 | 3.10e-65 | 221 |
| MS.gene066875.t1 | MTR_5g023790 | 47.679 | 237 | 118 | 2 | 462 | 692 | 144 | 380 | 4.66e-65 | 221 |
| MS.gene066875.t1 | MTR_5g088980 | 40.339 | 295 | 160 | 7 | 404 | 692 | 90 | 374 | 1.77e-63 | 217 |
| MS.gene066875.t1 | MTR_8g074000 | 47.748 | 222 | 113 | 1 | 474 | 692 | 163 | 384 | 3.72e-61 | 211 |
| MS.gene066875.t1 | MTR_7g089080 | 44.053 | 227 | 116 | 3 | 474 | 692 | 189 | 412 | 1.65e-54 | 202 |
| MS.gene066875.t1 | MTR_5g023790 | 47.778 | 180 | 86 | 1 | 474 | 645 | 162 | 341 | 1.07e-47 | 172 |
| MS.gene066875.t1 | MTR_1g011470 | 42.593 | 216 | 117 | 3 | 484 | 692 | 161 | 376 | 1.28e-47 | 174 |
| MS.gene066875.t1 | MTR_3g107970 | 41.743 | 218 | 120 | 3 | 482 | 692 | 211 | 428 | 1.43e-47 | 175 |
| MS.gene066875.t1 | MTR_2g102520 | 41.096 | 219 | 122 | 3 | 481 | 692 | 212 | 430 | 2.16e-46 | 172 |
| MS.gene066875.t1 | MTR_2g102550 | 37.768 | 233 | 137 | 4 | 468 | 692 | 150 | 382 | 5.21e-44 | 164 |
| MS.gene066875.t1 | MTR_3g088415 | 38.028 | 213 | 123 | 4 | 488 | 692 | 244 | 455 | 7.35e-43 | 162 |
| MS.gene066875.t1 | MTR_3g088405 | 37.736 | 212 | 125 | 3 | 488 | 692 | 247 | 458 | 1.96e-41 | 158 |
| MS.gene066875.t1 | MTR_3g088415 | 38.947 | 190 | 110 | 2 | 488 | 671 | 244 | 433 | 2.33e-40 | 154 |
| MS.gene066875.t1 | MTR_3g102510 | 37.438 | 203 | 118 | 4 | 484 | 678 | 74 | 275 | 1.85e-36 | 140 |
| MS.gene066875.t1 | MTR_3g060400 | 40.196 | 204 | 111 | 5 | 482 | 678 | 26 | 225 | 1.47e-35 | 136 |
| MS.gene066875.t1 | MTR_3g460990 | 40.196 | 204 | 111 | 5 | 482 | 678 | 26 | 225 | 1.47e-35 | 136 |
| MS.gene066875.t1 | MTR_1g018680 | 33.945 | 218 | 135 | 4 | 484 | 692 | 91 | 308 | 1.43e-34 | 135 |
| MS.gene066875.t1 | MTR_3g060770 | 37.799 | 209 | 119 | 4 | 477 | 678 | 25 | 229 | 2.53e-34 | 132 |
| MS.gene066875.t1 | MTR_3g102530 | 33.486 | 218 | 121 | 3 | 485 | 680 | 71 | 286 | 3.81e-34 | 134 |
| MS.gene066875.t1 | MTR_3g102520 | 35.321 | 218 | 128 | 5 | 481 | 687 | 88 | 303 | 4.41e-34 | 134 |
| MS.gene066875.t1 | MTR_3g060820 | 37.255 | 204 | 117 | 4 | 482 | 678 | 20 | 219 | 3.18e-33 | 129 |
| MS.gene066875.t1 | MTR_3g061680 | 37.143 | 210 | 121 | 4 | 476 | 678 | 14 | 219 | 3.85e-33 | 129 |
| MS.gene066875.t1 | MTR_3g060410 | 42.941 | 170 | 90 | 4 | 516 | 678 | 135 | 304 | 4.51e-33 | 131 |
| MS.gene066875.t1 | MTR_3g088415 | 41.259 | 143 | 83 | 1 | 488 | 629 | 244 | 386 | 1.35e-32 | 130 |
| MS.gene066875.t1 | MTR_3g102560 | 33.193 | 238 | 146 | 3 | 452 | 687 | 45 | 271 | 7.88e-32 | 126 |
| MS.gene066875.t1 | MTR_3g102540 | 36.548 | 197 | 116 | 5 | 496 | 687 | 23 | 215 | 4.09e-29 | 117 |
| MS.gene066875.t1 | MTR_6g045270 | 37.186 | 199 | 110 | 6 | 483 | 677 | 45 | 232 | 8.99e-26 | 108 |
| MS.gene066875.t1 | MTR_5g011900 | 27.094 | 203 | 141 | 3 | 451 | 649 | 40 | 239 | 1.42e-16 | 80.1 |
| MS.gene066875.t1 | MTR_1g032850 | 32.468 | 154 | 101 | 3 | 517 | 667 | 372 | 525 | 2.82e-16 | 82.8 |
| MS.gene066875.t1 | MTR_5g044040 | 38.667 | 75 | 46 | 0 | 538 | 612 | 7 | 81 | 2.05e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066875.t1 | AT1G16330 | 48.578 | 457 | 188 | 6 | 242 | 692 | 188 | 603 | 1.42e-131 | 403 |
| MS.gene066875.t1 | AT5G06150 | 47.489 | 219 | 114 | 1 | 474 | 692 | 172 | 389 | 3.27e-66 | 225 |
| MS.gene066875.t1 | AT1G20610 | 45.059 | 253 | 133 | 3 | 446 | 692 | 133 | 385 | 1.97e-65 | 223 |
| MS.gene066875.t1 | AT3G11520 | 43.019 | 265 | 139 | 4 | 430 | 692 | 112 | 366 | 1.39e-64 | 220 |
| MS.gene066875.t1 | AT1G76310 | 50.698 | 215 | 105 | 1 | 474 | 687 | 167 | 381 | 3.47e-64 | 218 |
| MS.gene066875.t1 | AT3G11520 | 43.019 | 265 | 139 | 4 | 430 | 692 | 112 | 366 | 7.07e-64 | 217 |
| MS.gene066875.t1 | AT1G76310 | 50.698 | 215 | 105 | 1 | 474 | 687 | 167 | 381 | 8.28e-64 | 218 |
| MS.gene066875.t1 | AT2G26760 | 38.276 | 290 | 155 | 7 | 408 | 692 | 69 | 339 | 2.12e-60 | 208 |
| MS.gene066875.t1 | AT1G76310 | 44.856 | 243 | 105 | 2 | 474 | 687 | 167 | 409 | 8.81e-58 | 203 |
| MS.gene066875.t1 | AT4G37490 | 41.880 | 234 | 132 | 3 | 462 | 692 | 141 | 373 | 2.81e-57 | 201 |
| MS.gene066875.t1 | AT5G11300 | 44.053 | 227 | 116 | 4 | 473 | 692 | 165 | 387 | 4.98e-53 | 189 |
| MS.gene066875.t1 | AT2G17620 | 45.339 | 236 | 127 | 1 | 459 | 692 | 144 | 379 | 2.51e-51 | 184 |
| MS.gene066875.t1 | AT4G35620 | 47.032 | 219 | 116 | 0 | 474 | 692 | 162 | 380 | 5.29e-51 | 182 |
| MS.gene066875.t1 | AT4G35620 | 47.032 | 219 | 116 | 0 | 474 | 692 | 162 | 380 | 1.05e-50 | 182 |
| MS.gene066875.t1 | AT5G25380 | 41.284 | 218 | 121 | 3 | 482 | 692 | 171 | 388 | 1.34e-47 | 174 |
| MS.gene066875.t1 | AT5G25380 | 41.284 | 218 | 121 | 3 | 482 | 692 | 171 | 388 | 1.46e-47 | 174 |
| MS.gene066875.t1 | AT5G25380 | 41.284 | 218 | 121 | 3 | 482 | 692 | 171 | 388 | 1.46e-47 | 174 |
| MS.gene066875.t1 | AT5G25380 | 41.284 | 218 | 121 | 3 | 482 | 692 | 177 | 394 | 1.96e-47 | 174 |
| MS.gene066875.t1 | AT1G76310 | 49.143 | 175 | 86 | 2 | 474 | 647 | 167 | 339 | 1.31e-46 | 169 |
| MS.gene066875.t1 | AT5G11300 | 43.662 | 213 | 109 | 4 | 473 | 678 | 165 | 373 | 3.74e-46 | 169 |
| MS.gene066875.t1 | AT5G06150 | 46.429 | 168 | 87 | 2 | 474 | 641 | 172 | 336 | 3.76e-46 | 167 |
| MS.gene066875.t1 | AT1G15570 | 38.053 | 226 | 133 | 3 | 474 | 692 | 178 | 403 | 1.13e-45 | 169 |
| MS.gene066875.t1 | AT1G80370 | 38.496 | 226 | 132 | 3 | 474 | 692 | 187 | 412 | 3.95e-45 | 168 |
| MS.gene066875.t1 | AT5G43080 | 38.278 | 209 | 123 | 3 | 484 | 688 | 87 | 293 | 4.22e-43 | 159 |
| MS.gene066875.t1 | AT1G44110 | 39.151 | 212 | 122 | 3 | 488 | 692 | 198 | 409 | 2.47e-42 | 160 |
| MS.gene066875.t1 | AT1G20590 | 49.669 | 151 | 76 | 0 | 542 | 692 | 63 | 213 | 1.28e-41 | 152 |
| MS.gene066875.t1 | AT5G43080 | 38.318 | 214 | 121 | 5 | 484 | 688 | 87 | 298 | 1.89e-41 | 155 |
| MS.gene066875.t1 | AT1G47230 | 33.080 | 263 | 154 | 5 | 439 | 692 | 63 | 312 | 6.14e-41 | 154 |
| MS.gene066875.t1 | AT1G47220 | 39.091 | 220 | 121 | 5 | 484 | 692 | 56 | 273 | 6.60e-40 | 150 |
| MS.gene066875.t1 | AT1G47210 | 36.818 | 220 | 126 | 5 | 484 | 692 | 102 | 319 | 1.43e-39 | 150 |
| MS.gene066875.t1 | AT1G47230 | 32.955 | 264 | 154 | 6 | 439 | 692 | 63 | 313 | 2.57e-39 | 149 |
| MS.gene066875.t1 | AT1G77390 | 37.850 | 214 | 122 | 4 | 488 | 692 | 182 | 393 | 7.52e-39 | 149 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 3.49e-27 | 116 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 259 | 401 | 3.55e-27 | 116 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 259 | 401 | 3.55e-27 | 116 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 248 | 390 | 3.91e-27 | 115 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 4.36e-27 | 115 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 4.36e-27 | 115 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 4.36e-27 | 115 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 4.36e-27 | 115 |
| MS.gene066875.t1 | AT1G34460 | 37.195 | 164 | 82 | 2 | 474 | 637 | 262 | 404 | 5.49e-27 | 115 |
| MS.gene066875.t1 | AT1G47210 | 42.857 | 91 | 49 | 2 | 484 | 571 | 102 | 192 | 6.96e-17 | 80.1 |
| MS.gene066875.t1 | AT1G14750 | 33.742 | 163 | 104 | 3 | 510 | 669 | 380 | 541 | 2.11e-16 | 83.6 |
| MS.gene066875.t1 | AT1G14750 | 33.742 | 163 | 104 | 3 | 510 | 669 | 380 | 541 | 2.95e-16 | 83.2 |
| MS.gene066875.t1 | AT1G14750 | 33.742 | 163 | 104 | 3 | 510 | 669 | 212 | 373 | 1.62e-15 | 79.7 |
| MS.gene066875.t1 | AT1G14750 | 33.742 | 163 | 103 | 4 | 510 | 669 | 212 | 372 | 1.11e-14 | 77.0 |
Find 152 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGGTTCTTACTTAATTT+TGG | 0.126058 | 8.4:-54315146 | None:intergenic |
| CTCTGCCAGTTACAGGTTTA+AGG | 0.127014 | 8.4:+54316966 | MS.gene066875:CDS |
| GTTTCGTTCTTGCATGTTTC+AGG | 0.152281 | 8.4:+54313703 | None:intergenic |
| AATTAGTAGGCACAGGTTTA+AGG | 0.156384 | 8.4:+54316611 | MS.gene066875:intron |
| ATCTTATACAACTTCATTAA+TGG | 0.209874 | 8.4:+54319317 | MS.gene066875:CDS |
| ATGCTATATATCAGTACTAT+TGG | 0.246809 | 8.4:+54319520 | MS.gene066875:CDS |
| AGGAAGTCTCTGCCAGTTAC+AGG | 0.257709 | 8.4:+54316959 | MS.gene066875:CDS |
| TTGCAGATTCTCAAGGAAAA+TGG | 0.281849 | 8.4:+54319423 | MS.gene066875:intron |
| CCTCAAAGTATGAGGACTTT+TGG | 0.284590 | 8.4:+54321148 | MS.gene066875:CDS |
| AACTAGTACAATTGCCTCTA+AGG | 0.304795 | 8.4:+54319044 | MS.gene066875:CDS |
| AACAAAACATGTGGAGCTTC+TGG | 0.315400 | 8.4:+54314951 | MS.gene066875:CDS |
| AACTTGAATCCGGTCAATTT+CGG | 0.318319 | 8.4:-54313915 | None:intergenic |
| TCAAGGAAAATGGTGAAGTT+AGG | 0.319988 | 8.4:+54319433 | MS.gene066875:CDS |
| TGATTTGTTCTGGCCTGATA+TGG | 0.332477 | 8.4:-54319016 | None:intergenic |
| CAGTTCCAAGGAGGGTAAAC+AGG | 0.338773 | 8.4:+54317319 | MS.gene066875:CDS |
| CTAAAATCTAAATCCATATC+AGG | 0.341068 | 8.4:+54319003 | MS.gene066875:CDS |
| TGCTCCTTGCATTAATTAGT+TGG | 0.354273 | 8.4:-54317714 | None:intergenic |
| CTTTGGATTAGCGGCAGTAT+TGG | 0.366466 | 8.4:-54314761 | None:intergenic |
| TGCTATATATCAGTACTATT+GGG | 0.372911 | 8.4:+54319521 | MS.gene066875:CDS |
| TCACAAAACCTTGTAGTTGT+AGG | 0.374254 | 8.4:-54316845 | None:intergenic |
| TTAATGCAAGGAGCAATCTA+TGG | 0.378840 | 8.4:+54317722 | MS.gene066875:CDS |
| ATTAGTAGGCACAGGTTTAA+GGG | 0.382551 | 8.4:+54316612 | MS.gene066875:intron |
| ATGTCTTTGACTTGCATCAT+TGG | 0.383091 | 8.4:-54319118 | None:intergenic |
| TCTGCCAGTTACAGGTTTAA+GGG | 0.386828 | 8.4:+54316967 | MS.gene066875:CDS |
| CTTGTAATTGTGGGTAACAT+AGG | 0.388170 | 8.4:-54317191 | None:intergenic |
| CTGTTTACCCTCCTTGGAAC+TGG | 0.389619 | 8.4:-54317318 | None:intergenic |
| TACCTATCCTTTGTGCCAAC+TGG | 0.389906 | 8.4:-54316679 | None:intergenic |
| GAAGCCCTTAAACCTGTAAC+TGG | 0.401913 | 8.4:-54316971 | None:intergenic |
| ACAGGTTTAAGGGCTTCTTT+AGG | 0.403015 | 8.4:+54316977 | MS.gene066875:CDS |
| TCTACCCTGTTTACCCTCCT+TGG | 0.405631 | 8.4:-54317324 | None:intergenic |
| GCCTCTGAATCGCGTCCAAC+TGG | 0.413116 | 8.4:+54313752 | MS.gene066875:CDS |
| TGCGGGGAGTATTGGTCAAC+TGG | 0.421771 | 8.4:+54320799 | MS.gene066875:CDS |
| GCTCCTTGCATTAATTAGTT+GGG | 0.422245 | 8.4:-54317713 | None:intergenic |
| TTAGAGGCAATTGTACTAGT+TGG | 0.428782 | 8.4:-54319042 | None:intergenic |
| GGCTCGAGTTGAGCCCAATC+TGG | 0.429781 | 8.4:-54313732 | None:intergenic |
| ATGTTTCTTCCAGCACTTCA+AGG | 0.433166 | 8.4:+54316939 | MS.gene066875:intron |
| TCCTCATATGCGGGGAGTAT+TGG | 0.436208 | 8.4:+54320791 | MS.gene066875:CDS |
| AAACAGTGGGCATCCCTTTG+AGG | 0.437482 | 8.4:+54315417 | MS.gene066875:CDS |
| AACCCCTTTGTTAGCTGCTA+TGG | 0.437995 | 8.4:-54314734 | None:intergenic |
| TTGATTGTGGTCTTAACAAC+AGG | 0.438316 | 8.4:-54318958 | None:intergenic |
| CCAAAAGTCCTCATACTTTG+AGG | 0.440603 | 8.4:-54321148 | None:intergenic |
| TTCGCAAACCTTGTAATTGT+GGG | 0.442913 | 8.4:-54317200 | None:intergenic |
| CTTGTAGTTGTAGGTAACAT+AGG | 0.444522 | 8.4:-54316836 | None:intergenic |
| AGGACTTCTGCAAGAGAAGT+TGG | 0.449064 | 8.4:+54314029 | MS.gene066875:intron |
| GGGAAATAAGTCACCTCCAT+TGG | 0.449283 | 8.4:-54316530 | None:intergenic |
| CTGACTTTCTTGAGGAGACT+TGG | 0.449935 | 8.4:-54318573 | None:intergenic |
| CGGAAGAATAGTTCATCAAA+TGG | 0.450007 | 8.4:+54316649 | MS.gene066875:CDS |
| ATTCGCAAACCTTGTAATTG+TGG | 0.452704 | 8.4:-54317201 | None:intergenic |
| TGTCTGTTCCAAGCACTTCA+AGG | 0.453793 | 8.4:+54317286 | MS.gene066875:intron |
| CCCTTTGAGGAAATCCTTCA+CGG | 0.456403 | 8.4:+54315430 | MS.gene066875:CDS |
| CAAGGAAAATGGTGAAGTTA+GGG | 0.456754 | 8.4:+54319434 | MS.gene066875:CDS |
| TGCCTTACCAGGGTTTCAGC+AGG | 0.457395 | 8.4:+54315383 | MS.gene066875:intron |
| TGAAGTAGTGGTGTCCATGA+TGG | 0.459792 | 8.4:-54322177 | None:intergenic |
| AAATGGTGAAGTTAGGGAGC+AGG | 0.462821 | 8.4:+54319440 | MS.gene066875:CDS |
| GTTTCAGGTAAAATCCAGAT+TGG | 0.463789 | 8.4:+54313718 | MS.gene066875:CDS |
| CTATGTTACCCACAATTACA+AGG | 0.468217 | 8.4:+54317192 | MS.gene066875:CDS |
| ATATCTGACAGTGACATTGT+TGG | 0.470336 | 8.4:+54321053 | MS.gene066875:CDS |
| GAAGTAGTGGTGTCCATGAT+GGG | 0.471578 | 8.4:-54322176 | None:intergenic |
| TGTATAAGATTTCCGACGGC+TGG | 0.471671 | 8.4:-54319304 | None:intergenic |
| GAGAAGTTGGCGCAGCTTCA+AGG | 0.471958 | 8.4:+54314042 | MS.gene066875:CDS |
| GATCAGAAGAATAATTCAAA+TGG | 0.472744 | 8.4:+54317001 | MS.gene066875:CDS |
| GTTATGAAGTTTCACAGATC+AGG | 0.476147 | 8.4:+54322211 | MS.gene066875:CDS |
| ACAGGTTTAAGGGTTTCCTT+AGG | 0.478290 | 8.4:+54316622 | MS.gene066875:intron |
| TGGAAGACCACTTTGGCCAC+TGG | 0.483759 | 8.4:-54317567 | None:intergenic |
| GTGCCATAGCAGCTAACAAA+GGG | 0.485499 | 8.4:+54314731 | MS.gene066875:CDS |
| TATACAAGAGATCAAATGCT+TGG | 0.491502 | 8.4:+54321305 | MS.gene066875:CDS |
| CATACTTTGAGGCCAACAAA+AGG | 0.492005 | 8.4:-54321137 | None:intergenic |
| AGGAAATATGAAAGCGAGAA+AGG | 0.495289 | 8.4:+54315063 | MS.gene066875:intron |
| TTTCAGGTAAAATCCAGATT+GGG | 0.496626 | 8.4:+54313719 | MS.gene066875:CDS |
| TGTTGTTAAGACCACAATCA+AGG | 0.496768 | 8.4:+54318960 | MS.gene066875:CDS |
| TTACATTCATCGTCTATACT+TGG | 0.497518 | 8.4:-54319471 | None:intergenic |
| CACTGTCAGATATAGTGTCT+CGG | 0.498442 | 8.4:-54321043 | None:intergenic |
| TTTGTTGGCCTCAAAGTATG+AGG | 0.498991 | 8.4:+54321140 | MS.gene066875:CDS |
| ACCCATCACCAACTTGAATC+CGG | 0.499561 | 8.4:-54313925 | None:intergenic |
| GACCGGATTCAAGTTGGTGA+TGG | 0.501396 | 8.4:+54313923 | MS.gene066875:CDS |
| GGCTGCACAATCAGATATGA+AGG | 0.502649 | 8.4:+54321889 | MS.gene066875:CDS |
| GTATATTACCTTTGGATTAG+CGG | 0.512604 | 8.4:-54314770 | None:intergenic |
| ATGACAACTGAATTATTCTC+AGG | 0.516778 | 8.4:-54319192 | None:intergenic |
| TGGGGAGAGCAGTTTGAAAA+CGG | 0.518960 | 8.4:+54319251 | MS.gene066875:CDS |
| ACTGTCAGATATAGTGTCTC+GGG | 0.522142 | 8.4:-54321042 | None:intergenic |
| ACCGGATTCAAGTTGGTGAT+GGG | 0.523190 | 8.4:+54313924 | MS.gene066875:CDS |
| CCTTAATTTGCAGATTCTCA+AGG | 0.524626 | 8.4:+54319416 | MS.gene066875:intron |
| CTATGTTACCTACAACTACA+AGG | 0.525004 | 8.4:+54316837 | MS.gene066875:CDS |
| TCTCAAGTACCTATCCTTTG+TGG | 0.526837 | 8.4:-54317038 | None:intergenic |
| TATGAAAGCGAGAAAGGCAT+TGG | 0.528115 | 8.4:+54315069 | MS.gene066875:CDS |
| ACCAATACTCCCCGCATATG+AGG | 0.531007 | 8.4:-54320792 | None:intergenic |
| AGAGATTACTCCTCATATGC+GGG | 0.536325 | 8.4:+54320782 | MS.gene066875:CDS |
| TACAATTGCCTCTAAGGATG+AGG | 0.537132 | 8.4:+54319050 | MS.gene066875:CDS |
| TCTTACTTGGAAGACCACTT+TGG | 0.541955 | 8.4:-54317574 | None:intergenic |
| TAAGGGTTTCCTTAGGTGAT+CGG | 0.541985 | 8.4:+54316629 | MS.gene066875:CDS |
| TGTAGAGCTTCCTTGAGCAT+TGG | 0.542467 | 8.4:-54315101 | None:intergenic |
| CAATTAGATGCTTCCAATGG+AGG | 0.543103 | 8.4:+54316517 | MS.gene066875:CDS |
| CCGTGAAGGATTTCCTCAAA+GGG | 0.543716 | 8.4:-54315430 | None:intergenic |
| TACCTTGACTGACTTTCTTG+AGG | 0.544981 | 8.4:-54318581 | None:intergenic |
| ACGAGTGAGATGCAGTTAGT+TGG | 0.548556 | 8.4:+54321105 | MS.gene066875:CDS |
| AAAGACATCAACCATCTGAT+GGG | 0.554208 | 8.4:+54319133 | MS.gene066875:CDS |
| ACCGTGAAGGATTTCCTCAA+AGG | 0.554678 | 8.4:-54315431 | None:intergenic |
| CAAAGACATCGACCATCTGA+TGG | 0.555227 | 8.4:+54319231 | MS.gene066875:CDS |
| ATACTTTGAGGCCAACAAAA+GGG | 0.555362 | 8.4:-54321136 | None:intergenic |
| CACTGCAAATAACCCCATCA+TGG | 0.557574 | 8.4:+54322163 | MS.gene066875:CDS |
| ATTCATCGTCTATACTTGGT+AGG | 0.558688 | 8.4:-54319467 | None:intergenic |
| ACTATTCTTCCGATCACCTA+AGG | 0.561914 | 8.4:-54316638 | None:intergenic |
| AAAGACATCGACCATCTGAT+GGG | 0.563380 | 8.4:+54319232 | MS.gene066875:CDS |
| GAAATTGACCGGATTCAAGT+TGG | 0.565766 | 8.4:+54313917 | MS.gene066875:CDS |
| ATTGGTCAACTGGCTAATTG+AGG | 0.566050 | 8.4:+54320809 | MS.gene066875:CDS |
| ATACTGCACTCCCCATCAGA+TGG | 0.569624 | 8.4:-54319144 | None:intergenic |
| GTTCAATTAGATGCTTCCAA+TGG | 0.571665 | 8.4:+54316514 | MS.gene066875:CDS |
| CAAAGACATCAACCATCTGA+TGG | 0.574777 | 8.4:+54319132 | MS.gene066875:CDS |
| AAGAGATCAAATGCTTGGAA+TGG | 0.575226 | 8.4:+54321310 | MS.gene066875:CDS |
| GTTCCCAACTAATTAATGCA+AGG | 0.576421 | 8.4:+54317710 | MS.gene066875:CDS |
| ACCAGTTGGACGCGATTCAG+AGG | 0.576483 | 8.4:-54313753 | None:intergenic |
| AATCAGTTGCCACAAAGGAT+AGG | 0.580793 | 8.4:+54317029 | MS.gene066875:CDS |
| CAATACTGCCGCTAATCCAA+AGG | 0.583084 | 8.4:+54314762 | MS.gene066875:CDS |
| AAACTGCTCTCCCCATCAGA+TGG | 0.584983 | 8.4:-54319243 | None:intergenic |
| GGAAGTCTCTGCCAGTTCCA+AGG | 0.587112 | 8.4:+54317307 | MS.gene066875:CDS |
| TACTTCCAATCGCAGGCACA+TGG | 0.589068 | 8.4:+54320726 | MS.gene066875:intron |
| GAAGGGTGATTTCAAAAGCA+TGG | 0.592171 | 8.4:+54314920 | MS.gene066875:intron |
| CAGAAGAATAATTCAAATGG+AGG | 0.592330 | 8.4:+54317004 | MS.gene066875:CDS |
| CTTCATATTCAACTAAGCAT+AGG | 0.594043 | 8.4:-54322083 | None:intergenic |
| GTCTCTGCCAGTTCCAAGGA+GGG | 0.594149 | 8.4:+54317311 | MS.gene066875:CDS |
| TCTTTCTGATAACAGTTCAG+TGG | 0.602262 | 8.4:+54319089 | MS.gene066875:CDS |
| GAGAAAAGTAACAAAACATG+TGG | 0.602918 | 8.4:+54314942 | MS.gene066875:CDS |
| AGGTTCAACCACTAAAACAG+TGG | 0.606699 | 8.4:+54315403 | MS.gene066875:CDS |
| GCTGATGTTACCAATGCTCA+AGG | 0.609500 | 8.4:+54315091 | MS.gene066875:CDS |
| GCTGATGCGCATAGTAGTGA+AGG | 0.610271 | 8.4:-54322120 | None:intergenic |
| CTCCTCAAGAAAGTCAGTCA+AGG | 0.612330 | 8.4:+54318579 | MS.gene066875:CDS |
| AACCAGTTGGCACAAAGGAT+AGG | 0.612355 | 8.4:+54316677 | MS.gene066875:CDS |
| TTATATGAAGCAGTGGGTGG+TGG | 0.612502 | 8.4:+54313872 | MS.gene066875:intron |
| TGGAAGACTCGAGTTAGTGA+TGG | 0.613219 | 8.4:+54317742 | MS.gene066875:CDS |
| AGGTCAATCAGTTGCCACAA+AGG | 0.614915 | 8.4:+54317024 | MS.gene066875:CDS |
| TCAAATGGAGGTCAACCAGT+TGG | 0.620772 | 8.4:+54316664 | MS.gene066875:CDS |
| CAGAGATTACTCCTCATATG+CGG | 0.620861 | 8.4:+54320781 | MS.gene066875:CDS |
| AAGTTGTATAAGATTTCCGA+CGG | 0.622303 | 8.4:-54319308 | None:intergenic |
| AGTTCCAAGGAGGGTAAACA+GGG | 0.627018 | 8.4:+54317320 | MS.gene066875:CDS |
| AGGTCAACCAGTTGGCACAA+AGG | 0.627713 | 8.4:+54316672 | MS.gene066875:CDS |
| GAAACAGCCAGTGGCCAAAG+TGG | 0.629869 | 8.4:+54317560 | MS.gene066875:CDS |
| AAGGAGTGGGAGCATTAAGA+CGG | 0.642469 | 8.4:-54321841 | None:intergenic |
| AAGAATAGTTCATCAAATGG+AGG | 0.648198 | 8.4:+54316652 | MS.gene066875:CDS |
| GGTTCAACCACTAAAACAGT+GGG | 0.650460 | 8.4:+54315404 | MS.gene066875:CDS |
| AGATGTAGAAGCCTTGATTG+TGG | 0.654920 | 8.4:-54318971 | None:intergenic |
| TGTGACTTTCCATACCGTGA+AGG | 0.662185 | 8.4:-54315444 | None:intergenic |
| AAGTAGTGGTGTCCATGATG+GGG | 0.666437 | 8.4:-54322175 | None:intergenic |
| AAGACATCAACCATCTGATG+GGG | 0.669550 | 8.4:+54319134 | MS.gene066875:CDS |
| TCAGTACTATTGGGTTACTG+AGG | 0.675513 | 8.4:+54319530 | MS.gene066875:CDS |
| AGTCTCTGCCAGTTCCAAGG+AGG | 0.676674 | 8.4:+54317310 | MS.gene066875:CDS |
| AAGACATCGACCATCTGATG+GGG | 0.684383 | 8.4:+54319233 | MS.gene066875:CDS |
| GAGATTACTCCTCATATGCG+GGG | 0.688505 | 8.4:+54320783 | MS.gene066875:CDS |
| AGTGCCATAGCAGCTAACAA+AGG | 0.688688 | 8.4:+54314730 | MS.gene066875:CDS |
| GGTAGAGATGAATAACACAA+AGG | 0.692382 | 8.4:+54317341 | MS.gene066875:CDS |
| GGAAGTTCTGAAACAGCCAG+TGG | 0.695203 | 8.4:+54317551 | MS.gene066875:CDS |
| TCAAGGCTTCTACATCTCAG+AGG | 0.717453 | 8.4:+54318977 | MS.gene066875:CDS |
| TGCCATAGCAGCTAACAAAG+GGG | 0.737862 | 8.4:+54314732 | MS.gene066875:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 54313716 | 54322232 | 54313716 | ID=MS.gene066875 |
| chr8.4 | mRNA | 54313716 | 54322232 | 54313716 | ID=MS.gene066875.t1;Parent=MS.gene066875 |
| chr8.4 | exon | 54313716 | 54313773 | 54313716 | ID=MS.gene066875.t1.exon1;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54313716 | 54313773 | 54313716 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54313885 | 54313954 | 54313885 | ID=MS.gene066875.t1.exon2;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54313885 | 54313954 | 54313885 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54314031 | 54314063 | 54314031 | ID=MS.gene066875.t1.exon3;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54314031 | 54314063 | 54314031 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54314726 | 54314783 | 54314726 | ID=MS.gene066875.t1.exon4;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54314726 | 54314783 | 54314726 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54314924 | 54314972 | 54314924 | ID=MS.gene066875.t1.exon5;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54314924 | 54314972 | 54314924 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54315065 | 54315155 | 54315065 | ID=MS.gene066875.t1.exon6;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54315065 | 54315155 | 54315065 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54315394 | 54315451 | 54315394 | ID=MS.gene066875.t1.exon7;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54315394 | 54315451 | 54315394 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54316481 | 54316538 | 54316481 | ID=MS.gene066875.t1.exon8;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54316481 | 54316538 | 54316481 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54316626 | 54316698 | 54316626 | ID=MS.gene066875.t1.exon9;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54316626 | 54316698 | 54316626 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54316823 | 54316858 | 54316823 | ID=MS.gene066875.t1.exon10;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54316823 | 54316858 | 54316823 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54316952 | 54317050 | 54316952 | ID=MS.gene066875.t1.exon11;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54316952 | 54317050 | 54316952 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54317178 | 54317213 | 54317178 | ID=MS.gene066875.t1.exon12;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54317178 | 54317213 | 54317178 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54317299 | 54317362 | 54317299 | ID=MS.gene066875.t1.exon13;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54317299 | 54317362 | 54317299 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54317542 | 54317590 | 54317542 | ID=MS.gene066875.t1.exon14;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54317542 | 54317590 | 54317542 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54317683 | 54317776 | 54317683 | ID=MS.gene066875.t1.exon15;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54317683 | 54317776 | 54317683 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54318555 | 54318600 | 54318555 | ID=MS.gene066875.t1.exon16;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54318555 | 54318600 | 54318555 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54318958 | 54319350 | 54318958 | ID=MS.gene066875.t1.exon17;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54318958 | 54319350 | 54318958 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54319429 | 54319551 | 54319429 | ID=MS.gene066875.t1.exon18;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54319429 | 54319551 | 54319429 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54320741 | 54320830 | 54320741 | ID=MS.gene066875.t1.exon19;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54320741 | 54320830 | 54320741 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54321018 | 54321179 | 54321018 | ID=MS.gene066875.t1.exon20;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54321018 | 54321179 | 54321018 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54321272 | 54321331 | 54321272 | ID=MS.gene066875.t1.exon21;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54321272 | 54321331 | 54321272 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54321812 | 54321910 | 54321812 | ID=MS.gene066875.t1.exon22;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54321812 | 54321910 | 54321812 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| chr8.4 | exon | 54322054 | 54322232 | 54322054 | ID=MS.gene066875.t1.exon23;Parent=MS.gene066875.t1 |
| chr8.4 | CDS | 54322054 | 54322232 | 54322054 | ID=cds.MS.gene066875.t1;Parent=MS.gene066875.t1 |
| Gene Sequence |
| Protein sequence |
