AlfalfaGEDB | MS.gene67743

M. sativa cultivar XinJiangDaYe / MS.gene67743

KEGG: K09549
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF01920.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67743.t1	XP_003598099.1	100	147	0	0	1	147	1	147	7.60E-70	273.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67743.t1	Q9LJ98	70.7	150	39	3	1	147	1	148	3.8e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67743.t1	G7J0A7	100.0	147	0	0	1	147	1	147	5.5e-70	273.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene67743	MS.gene071261	PPI
MS.gene066877	MS.gene67743	PPI
MS.gene28046	MS.gene67743	PPI
MS.gene67743	MS.gene058245	PPI
MS.gene67743	MS.gene037362	PPI
MS.gene67743	MS.gene09551	PPI
MS.gene67743	MS.gene047578	PPI
MS.gene043645	MS.gene67743	PPI
MS.gene67743	MS.gene75567	PPI
MS.gene67743	MS.gene23930	PPI
MS.gene003428	MS.gene67743	PPI
MS.gene67743	MS.gene002442	PPI
MS.gene67743	MS.gene79126	PPI
MS.gene67743	MS.gene043595	PPI
MS.gene43992	MS.gene67743	PPI
MS.gene67743	MS.gene69947	PPI
MS.gene030840	MS.gene67743	PPI
MS.gene043593	MS.gene67743	PPI
MS.gene015206	MS.gene67743	PPI
MS.gene67743	MS.gene20253	PPI
MS.gene67743	MS.gene44510	PPI
MS.gene67743	MS.gene061811	PPI
MS.gene67743	MS.gene78457	PPI
MS.gene045765	MS.gene67743	PPI
MS.gene67743	MS.gene015213	PPI
MS.gene064353	MS.gene67743	PPI
MS.gene67743	MS.gene050877	PPI
MS.gene67743	MS.gene01284	PPI
MS.gene67743	MS.gene045227	PPI
MS.gene67743	MS.gene40225	PPI
MS.gene67743	MS.gene01697	PPI
MS.gene67743	MS.gene20245	PPI
MS.gene67743	MS.gene066875	PPI
MS.gene67743	MS.gene068943	PPI
MS.gene67743	MS.gene051698	PPI
MS.gene67743	MS.gene01091	PPI
MS.gene67743	MS.gene029211	PPI
MS.gene67743	MS.gene75501	PPI
MS.gene002306	MS.gene67743	PPI
MS.gene67743	MS.gene93131	PPI
MS.gene67743	MS.gene015212	PPI
MS.gene02265	MS.gene67743	PPI
MS.gene67743	MS.gene035825	PPI
MS.gene67743	MS.gene36102	PPI
MS.gene67743	MS.gene066876	PPI
MS.gene57177	MS.gene67743	PPI
MS.gene67743	MS.gene84699	PPI
MS.gene67743	MS.gene77306	PPI
MS.gene67743	MS.gene43369	PPI
MS.gene42750	MS.gene67743	PPI
MS.gene035826	MS.gene67743	PPI
MS.gene67743	MS.gene01425	PPI
MS.gene67743	MS.gene67725	PPI
MS.gene000365	MS.gene67743	PPI
MS.gene066552	MS.gene67743	PPI
MS.gene67743	MS.gene55980	PPI
MS.gene041917	MS.gene67743	PPI
MS.gene064351	MS.gene67743	PPI
MS.gene67743	MS.gene69089	PPI
MS.gene67743	MS.gene09540	PPI
MS.gene09552	MS.gene67743	PPI
MS.gene67743	MS.gene23694	PPI
MS.gene67743	MS.gene015202	PPI
MS.gene67743	MS.gene73093	PPI
MS.gene67743	MS.gene066551	PPI
MS.gene67743	MS.gene00146	PPI
MS.gene011754	MS.gene67743	PPI
MS.gene67743	MS.gene74586	PPI
MS.gene41343	MS.gene67743	PPI
MS.gene67743	MS.gene066566	PPI
MS.gene67743	MS.gene57325	PPI
MS.gene99872	MS.gene67743	PPI
MS.gene67743	MS.gene20252	PPI
MS.gene043643	MS.gene67743	PPI
MS.gene03171	MS.gene67743	PPI
MS.gene67743	MS.gene067039	PPI
MS.gene65650	MS.gene67743	PPI
MS.gene78156	MS.gene67743	PPI
MS.gene014578	MS.gene67743	PPI
MS.gene035828	MS.gene67743	PPI
MS.gene53708	MS.gene67743	PPI
MS.gene67743	MS.gene02047	PPI
MS.gene003245	MS.gene67743	PPI
MS.gene67743	MS.gene09543	PPI
MS.gene045141	MS.gene67743	PPI
MS.gene67743	MS.gene98781	PPI
MS.gene058013	MS.gene67743	PPI
MS.gene67743	MS.gene57178	PPI
MS.gene67743	MS.gene79100	PPI
MS.gene67743	MS.gene005125	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67743.t1	MTR_3g007250	100.000	147	0	0	1	147	1	147	4.41e-105	296

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67743.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201
MS.gene67743.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201

Find 44 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTCAAGTTCCGTTATTT+TGG	0.192430	3.3:-3410357	None:intergenic
GCAACTGCTTGTTCATTTAC+TGG	0.248625	3.3:-3410290	None:intergenic
TACTGGCTCCTTGCGATCTT+CGG	0.331035	3.3:-3410273	None:intergenic
CAAAATAACGGAACTTGAAA+TGG	0.346568	3.3:+3410358	MS.gene67743:CDS
AGCAGGCAGGACCTCTTTGA+TGG	0.408930	3.3:-3410477	None:intergenic
AGACATTGGAAAAGAAGAAA+AGG	0.418775	3.3:+3410549	MS.gene67743:CDS
TTGAGACTAAATATAAAATC+AGG	0.420368	3.3:+3410585	MS.gene67743:CDS
GGAGTACTTGTGGGCCCTGC+GGG	0.425415	3.3:+3410668	MS.gene67743:CDS
TCTTTATTCCGCTGCACAGC+AGG	0.438536	3.3:-3410494	None:intergenic
AGACGATGCTATCGAATGAT+TGG	0.440454	3.3:+3410434	MS.gene67743:CDS
TTCGATAGCATCGTCTAGAT+TGG	0.458077	3.3:-3410427	None:intergenic
GACATTGGAAAAGAAGAAAA+GGG	0.500685	3.3:+3410550	MS.gene67743:CDS
GTGTAGAATCATGGCTAGTA+AGG	0.508954	3.3:+3410250	None:intergenic
GCTGAGGTGAACGATGAATC+TGG	0.515292	3.3:+3410623	MS.gene67743:CDS
CCAAATTTACACCAAAATAA+CGG	0.527386	3.3:+3410346	MS.gene67743:CDS
AGATTGGTCTAGCGGCTGAA+TGG	0.531922	3.3:-3410411	None:intergenic
TGTCATTCGCTACCACCCGC+AGG	0.539315	3.3:-3410683	None:intergenic
TATTCCGCTGCACAGCAGGC+AGG	0.540606	3.3:-3410490	None:intergenic
TGCAAGGCTTAACGAGACAT+TGG	0.541862	3.3:+3410535	MS.gene67743:CDS
GTACTTGTGGGCCCTGCGGG+TGG	0.543152	3.3:+3410671	MS.gene67743:CDS
GAACGATGAATCTGGTAAGA+AGG	0.544140	3.3:+3410631	MS.gene67743:CDS
TCAGCTCAAGGAGTACTTGT+GGG	0.554339	3.3:+3410659	MS.gene67743:CDS
AAGAAGGAAGGTTCAGCTCA+AGG	0.558366	3.3:+3410647	MS.gene67743:CDS
GCTGTGCAGCGGAATAAAGA+AGG	0.563818	3.3:+3410497	MS.gene67743:CDS
CGATGCTATCGAATGATTGG+AGG	0.563926	3.3:+3410437	MS.gene67743:CDS
TTCATCGTTCACCTCAGCAT+CGG	0.575473	3.3:-3410618	None:intergenic
GATGAATCTGGTAAGAAGGA+AGG	0.587326	3.3:+3410635	MS.gene67743:CDS
CTAAATATAAAATCAGGATG+AGG	0.587775	3.3:+3410591	MS.gene67743:CDS
GATGAGGAAGGCCGATGCTG+AGG	0.588771	3.3:+3410607	MS.gene67743:CDS
AGGTCCTGCCTGCTGTGCAG+CGG	0.589956	3.3:+3410486	MS.gene67743:CDS
GACTTGAAGAGGTTGTTGCA+AGG	0.593695	3.3:+3410519	MS.gene67743:CDS
GTCATTCGCTACCACCCGCA+GGG	0.594608	3.3:-3410682	None:intergenic
ATATAAAATCAGGATGAGGA+AGG	0.596264	3.3:+3410595	MS.gene67743:CDS
TAGTAAGGCCGAAGATCGCA+AGG	0.597879	3.3:+3410265	MS.gene67743:CDS
AGGAGTACTTGTGGGCCCTG+CGG	0.600783	3.3:+3410667	MS.gene67743:CDS
TTCAGCTCAAGGAGTACTTG+TGG	0.603923	3.3:+3410658	MS.gene67743:CDS
AATGATTGGAGGGGTGCTTG+TGG	0.605277	3.3:+3410448	MS.gene67743:CDS
GATGCTATCGAATGATTGGA+GGG	0.617244	3.3:+3410438	MS.gene67743:CDS
GGAAGTCAGTGAGCACTCGT+TGG	0.621570	3.3:+3410379	MS.gene67743:CDS
CAAATATGTATGCAGCTATG+AGG	0.631644	3.3:+3410312	MS.gene67743:CDS
CATCGTCTAGATTGGTCTAG+CGG	0.643268	3.3:-3410419	None:intergenic
GAATAAAGAAGGACTTGAAG+AGG	0.645797	3.3:+3410508	MS.gene67743:CDS
TGTGGAAAGAACCATCAAAG+AGG	0.692372	3.3:+3410466	MS.gene67743:CDS
ATGCTATCGAATGATTGGAG+GGG	0.721195	3.3:+3410439	MS.gene67743:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAGACTAAATATAAAATC+AGG	+	chr3.3:3410585-3410604	MS.gene67743:CDS	20.0%
!	CCAAATTTACACCAAAATAA+CGG	+	chr3.3:3410346-3410365	MS.gene67743:CDS	25.0%
!	CTAAATATAAAATCAGGATG+AGG	+	chr3.3:3410591-3410610	MS.gene67743:CDS	25.0%
	AGACATTGGAAAAGAAGAAA+AGG	+	chr3.3:3410549-3410568	MS.gene67743:CDS	30.0%
	ATATAAAATCAGGATGAGGA+AGG	+	chr3.3:3410595-3410614	MS.gene67743:CDS	30.0%
	GACATTGGAAAAGAAGAAAA+GGG	+	chr3.3:3410550-3410569	MS.gene67743:CDS	30.0%
!	CAAAATAACGGAACTTGAAA+TGG	+	chr3.3:3410358-3410377	MS.gene67743:CDS	30.0%
!!	TTTTGGTGTAAATTTGGTTG+AGG	-	chr3.3:3410343-3410362	None:intergenic	30.0%
!!!	CATTTCAAGTTCCGTTATTT+TGG	-	chr3.3:3410360-3410379	None:intergenic	30.0%
!!!	CCGTTATTTTGGTGTAAATT+TGG	-	chr3.3:3410349-3410368	None:intergenic	30.0%
	CAAATATGTATGCAGCTATG+AGG	+	chr3.3:3410312-3410331	MS.gene67743:CDS	35.0%
	GAATAAAGAAGGACTTGAAG+AGG	+	chr3.3:3410508-3410527	MS.gene67743:CDS	35.0%
	AGACGATGCTATCGAATGAT+TGG	+	chr3.3:3410434-3410453	MS.gene67743:CDS	40.0%
	ATGCTATCGAATGATTGGAG+GGG	+	chr3.3:3410439-3410458	MS.gene67743:CDS	40.0%
	GAACGATGAATCTGGTAAGA+AGG	+	chr3.3:3410631-3410650	MS.gene67743:CDS	40.0%
	GATGAATCTGGTAAGAAGGA+AGG	+	chr3.3:3410635-3410654	MS.gene67743:CDS	40.0%
	GATGCTATCGAATGATTGGA+GGG	+	chr3.3:3410438-3410457	MS.gene67743:CDS	40.0%
	GCAACTGCTTGTTCATTTAC+TGG	-	chr3.3:3410293-3410312	None:intergenic	40.0%
	TGTGGAAAGAACCATCAAAG+AGG	+	chr3.3:3410466-3410485	MS.gene67743:CDS	40.0%
	TTCGATAGCATCGTCTAGAT+TGG	-	chr3.3:3410430-3410449	None:intergenic	40.0%
	AAGAAGGAAGGTTCAGCTCA+AGG	+	chr3.3:3410647-3410666	MS.gene67743:CDS	45.0%
	CGATGCTATCGAATGATTGG+AGG	+	chr3.3:3410437-3410456	MS.gene67743:CDS	45.0%
	GACTTGAAGAGGTTGTTGCA+AGG	+	chr3.3:3410519-3410538	MS.gene67743:CDS	45.0%
	TGCAAGGCTTAACGAGACAT+TGG	+	chr3.3:3410535-3410554	MS.gene67743:CDS	45.0%
	TTCATCGTTCACCTCAGCAT+CGG	-	chr3.3:3410621-3410640	None:intergenic	45.0%
!!	CATCGTCTAGATTGGTCTAG+CGG	-	chr3.3:3410422-3410441	None:intergenic	45.0%
!!	TCAGCTCAAGGAGTACTTGT+GGG	+	chr3.3:3410659-3410678	MS.gene67743:CDS	45.0%
!!	TTCAGCTCAAGGAGTACTTG+TGG	+	chr3.3:3410658-3410677	MS.gene67743:CDS	45.0%
	GCTGAGGTGAACGATGAATC+TGG	+	chr3.3:3410623-3410642	MS.gene67743:CDS	50.0%
	GCTGTGCAGCGGAATAAAGA+AGG	+	chr3.3:3410497-3410516	MS.gene67743:CDS	50.0%
	TAGTAAGGCCGAAGATCGCA+AGG	+	chr3.3:3410265-3410284	MS.gene67743:CDS	50.0%
	TCTTTATTCCGCTGCACAGC+AGG	-	chr3.3:3410497-3410516	None:intergenic	50.0%
!!	AATGATTGGAGGGGTGCTTG+TGG	+	chr3.3:3410448-3410467	MS.gene67743:CDS	50.0%
!!	AGATTGGTCTAGCGGCTGAA+TGG	-	chr3.3:3410414-3410433	None:intergenic	50.0%
!!	TACTGGCTCCTTGCGATCTT+CGG	-	chr3.3:3410276-3410295	None:intergenic	50.0%
	AGCAGGCAGGACCTCTTTGA+TGG	-	chr3.3:3410480-3410499	None:intergenic	55.0%
	GGAAGTCAGTGAGCACTCGT+TGG	+	chr3.3:3410379-3410398	MS.gene67743:CDS	55.0%
	GATGAGGAAGGCCGATGCTG+AGG	+	chr3.3:3410607-3410626	MS.gene67743:CDS	60.0%
	TATTCCGCTGCACAGCAGGC+AGG	-	chr3.3:3410493-3410512	None:intergenic	60.0%
!!	AGGAGTACTTGTGGGCCCTG+CGG	+	chr3.3:3410667-3410686	MS.gene67743:CDS	60.0%
	AGGTCCTGCCTGCTGTGCAG+CGG	+	chr3.3:3410486-3410505	MS.gene67743:CDS	65.0%
!	GGAGTACTTGTGGGCCCTGC+GGG	+	chr3.3:3410668-3410687	MS.gene67743:CDS	65.0%
	GTACTTGTGGGCCCTGCGGG+TGG	+	chr3.3:3410671-3410690	MS.gene67743:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	3410260	3410703	3410260	ID=MS.gene67743
chr3.3	mRNA	3410260	3410703	3410260	ID=MS.gene67743.t1;Parent=MS.gene67743
chr3.3	exon	3410260	3410703	3410260	ID=MS.gene67743.t1.exon1;Parent=MS.gene67743.t1
chr3.3	CDS	3410260	3410703	3410260	ID=cds.MS.gene67743.t1;Parent=MS.gene67743.t1

Gene Sequence

>MS.gene67743

ATGGCTAGTAAGGCCGAAGATCGCAAGGAGCCAGTAAATGAACAAGCAGTTGCAAATATGTATGCAGCTATGAGGTCAGACCTCAACCAAATTTACACCAAAATAACGGAACTTGAAATGGAAGTCAGTGAGCACTCGTTGGTTCTTAATGCCATTCAGCCGCTAGACCAATCTAGACGATGCTATCGAATGATTGGAGGGGTGCTTGTGGAAAGAACCATCAAAGAGGTCCTGCCTGCTGTGCAGCGGAATAAAGAAGGACTTGAAGAGGTTGTTGCAAGGCTTAACGAGACATTGGAAAAGAAGAAAAGGGAAATTGCTGAGTTTGAGACTAAATATAAAATCAGGATGAGGAAGGCCGATGCTGAGGTGAACGATGAATCTGGTAAGAAGGAAGGTTCAGCTCAAGGAGTACTTGTGGGCCCTGCGGGTGGTAGCGAATGA

Protein sequence

>MS.gene67743.t1

MASKAEDRKEPVNEQAVANMYAAMRSDLNQIYTKITELEMEVSEHSLVLNAIQPLDQSRRCYRMIGGVLVERTIKEVLPAVQRNKEGLEEVVARLNETLEKKKREIAEFETKYKIRMRKADAEVNDESGKKEGSAQGVLVGPAGGSE