AlfalfaGEDB | MS.gene009372

M. sativa cultivar XinJiangDaYe / MS.gene009372

KEGG: K09549
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF01920.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009372.t1	XP_003598099.1	100	147	0	0	1	147	1	147	7.60E-70	273.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009372.t1	Q9LJ98	70.7	150	39	3	1	147	1	148	3.8e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009372.t1	G7J0A7	100.0	147	0	0	1	147	1	147	5.5e-70	273.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene009372	MS.gene00146	PPI
MS.gene009372	MS.gene57178	PPI
MS.gene009372	MS.gene79100	PPI
MS.gene009372	MS.gene005125	PPI
MS.gene009372	MS.gene41343	PPI
MS.gene009372	MS.gene066566	PPI
MS.gene009372	MS.gene53708	PPI
MS.gene009372	MS.gene071261	PPI
MS.gene009372	MS.gene20252	PPI
MS.gene009372	MS.gene067039	PPI
MS.gene009372	MS.gene045141	PPI
MS.gene009372	MS.gene064353	PPI
MS.gene009372	MS.gene047578	PPI
MS.gene009372	MS.gene23930	PPI
MS.gene009372	MS.gene043595	PPI
MS.gene009372	MS.gene02047	PPI
MS.gene009372	MS.gene09543	PPI
MS.gene009372	MS.gene20253	PPI
MS.gene57177	MS.gene009372	PPI
MS.gene009372	MS.gene03171	PPI
MS.gene009372	MS.gene98781	PPI
MS.gene42750	MS.gene009372	PPI
MS.gene035826	MS.gene009372	PPI
MS.gene009372	MS.gene78457	PPI
MS.gene000365	MS.gene009372	PPI
MS.gene066552	MS.gene009372	PPI
MS.gene009372	MS.gene015213	PPI
MS.gene041917	MS.gene009372	PPI
MS.gene009372	MS.gene058245	PPI
MS.gene009372	MS.gene037362	PPI
MS.gene09552	MS.gene009372	PPI
MS.gene009372	MS.gene011754	PPI
MS.gene009372	MS.gene045765	PPI
MS.gene009372	MS.gene01284	PPI
MS.gene009372	MS.gene75567	PPI
MS.gene009372	MS.gene002442	PPI
MS.gene009372	MS.gene066875	PPI
MS.gene009372	MS.gene79126	PPI
MS.gene009372	MS.gene068943	PPI
MS.gene99872	MS.gene009372	PPI
MS.gene009372	MS.gene01091	PPI
MS.gene009372	MS.gene69947	PPI
MS.gene009372	MS.gene043645	PPI
MS.gene009372	MS.gene44510	PPI
MS.gene009372	MS.gene061811	PPI
MS.gene009372	MS.gene015212	PPI
MS.gene009372	MS.gene066876	PPI
MS.gene65650	MS.gene009372	PPI
MS.gene009372	MS.gene84699	PPI
MS.gene009372	MS.gene064351	PPI
MS.gene014578	MS.gene009372	PPI
MS.gene035828	MS.gene009372	PPI
MS.gene009372	MS.gene050877	PPI
MS.gene009372	MS.gene67725	PPI
MS.gene003245	MS.gene009372	PPI
MS.gene009372	MS.gene003428	PPI
MS.gene009372	MS.gene043593	PPI
MS.gene009372	MS.gene69089	PPI
MS.gene058013	MS.gene009372	PPI
MS.gene009372	MS.gene09540	PPI
MS.gene009372	MS.gene045227	PPI
MS.gene009372	MS.gene40225	PPI
MS.gene009372	MS.gene066877	PPI
MS.gene009372	MS.gene73093	PPI
MS.gene009372	MS.gene01697	PPI
MS.gene009372	MS.gene78156	PPI
MS.gene009372	MS.gene20245	PPI
MS.gene009372	MS.gene066551	PPI
MS.gene009372	MS.gene051698	PPI
MS.gene009372	MS.gene74586	PPI
MS.gene009372	MS.gene029211	PPI
MS.gene009372	MS.gene02265	PPI
MS.gene009372	MS.gene75501	PPI
MS.gene009372	MS.gene28046	PPI
MS.gene009372	MS.gene93131	PPI
MS.gene009372	MS.gene57325	PPI
MS.gene009372	MS.gene035825	PPI
MS.gene009372	MS.gene36102	PPI
MS.gene009372	MS.gene002306	PPI
MS.gene009372	MS.gene043643	PPI
MS.gene009372	MS.gene77306	PPI
MS.gene009372	MS.gene43369	PPI
MS.gene009372	MS.gene09551	PPI
MS.gene009372	MS.gene01425	PPI
MS.gene43992	MS.gene009372	PPI
MS.gene030840	MS.gene009372	PPI
MS.gene015206	MS.gene009372	PPI
MS.gene009372	MS.gene55980	PPI
MS.gene009372	MS.gene23694	PPI
MS.gene009372	MS.gene015202	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009372.t1	MTR_3g007250	100.000	147	0	0	1	147	1	147	4.41e-105	296

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009372.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201
MS.gene009372.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201

Find 44 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTCAAGTTCCGTTATTT+TGG	0.192430	3.1:-2696847	None:intergenic
GCAACTGCTTGTTCATTTAC+TGG	0.248625	3.1:-2696780	None:intergenic
TACTGGCTCCTTGCGATCTT+CGG	0.331035	3.1:-2696763	None:intergenic
CAAAATAACGGAACTTGAAA+TGG	0.346568	3.1:+2696848	MS.gene009372:CDS
CGGCGTCTGCCTTCCTCATC+CGG	0.403003	3.1:-2697094	None:intergenic
AGACATTGGAAAAGAAGAAA+AGG	0.418775	3.1:+2697039	MS.gene009372:CDS
GGAGTACTTGTGGGCCCTGC+GGG	0.425415	3.1:+2697158	MS.gene009372:CDS
AGCAGGCAAGACCTCTTTGA+TGG	0.434394	3.1:-2696967	None:intergenic
AGACGATGCTATCGAATGAT+TGG	0.440454	3.1:+2696924	MS.gene009372:CDS
TTCGATAGCATCGTCTAGAT+TGG	0.460560	3.1:-2696917	None:intergenic
TGTCATTCGCTGCCACCCGC+AGG	0.461645	3.1:-2697173	None:intergenic
TCTTAATGCCATTCAGCCGC+TGG	0.472683	3.1:+2696893	MS.gene009372:CDS
AGATTGGTCCAGCGGCTGAA+TGG	0.484859	3.1:-2696901	None:intergenic
TCTTTATTTCGCTGCACAGC+AGG	0.487561	3.1:-2696984	None:intergenic
TTGAGACTAAATATAAAATC+CGG	0.490885	3.1:+2697075	MS.gene009372:CDS
GACATTGGAAAAGAAGAAAA+GGG	0.500685	3.1:+2697040	MS.gene009372:CDS
GTGTAGAATCATGGCTAGTA+AGG	0.508954	3.1:+2696740	None:intergenic
GCCGAGGTGAATGATGAATC+TGG	0.515159	3.1:+2697113	MS.gene009372:CDS
GAATGATGAATCTGGTAAGA+AGG	0.524633	3.1:+2697121	MS.gene009372:CDS
CCAAATTTACACCAAAATAA+CGG	0.527386	3.1:+2696836	MS.gene009372:CDS
GTACTTGTGGGCCCTGCGGG+TGG	0.538420	3.1:+2697161	MS.gene009372:CDS
TGCGAGGCTTAACGAGACAT+TGG	0.548123	3.1:+2697025	MS.gene009372:CDS
GTCATTCGCTGCCACCCGCA+GGG	0.549383	3.1:-2697172	None:intergenic
TCAGCTCAAGGAGTACTTGT+GGG	0.554339	3.1:+2697149	MS.gene009372:CDS
AAGAAGGAAGGTTCAGCTCA+AGG	0.558366	3.1:+2697137	MS.gene009372:CDS
CGATGCTATCGAATGATTGG+AGG	0.563926	3.1:+2696927	MS.gene009372:CDS
GATGAATCTGGTAAGAAGGA+AGG	0.576871	3.1:+2697125	MS.gene009372:CDS
ATATAAAATCCGGATGAGGA+AGG	0.583660	3.1:+2697085	MS.gene009372:CDS
GCTGTGCAGCGAAATAAAGA+AGG	0.585102	3.1:+2696987	MS.gene009372:CDS
CTAAATATAAAATCCGGATG+AGG	0.587775	3.1:+2697081	MS.gene009372:CDS
TAGTAAGGCCGAAGATCGCA+AGG	0.597879	3.1:+2696755	MS.gene009372:CDS
GGAAGTCAGCGAGCACTCGT+TGG	0.600111	3.1:+2696869	MS.gene009372:CDS
AGGAGTACTTGTGGGCCCTG+CGG	0.600783	3.1:+2697157	MS.gene009372:CDS
TTCAGCTCAAGGAGTACTTG+TGG	0.603923	3.1:+2697148	MS.gene009372:CDS
AATGATTGGAGGGGTGCTTG+TGG	0.605277	3.1:+2696938	MS.gene009372:CDS
AAATAAAGAAGGACTTGAAG+AGG	0.608952	3.1:+2696998	MS.gene009372:CDS
GATGCTATCGAATGATTGGA+GGG	0.617244	3.1:+2696928	MS.gene009372:CDS
ACCAGATTCATCATTCACCT+CGG	0.628665	3.1:-2697114	None:intergenic
GACTTGAAGAGGTTGTTGCG+AGG	0.642407	3.1:+2697009	MS.gene009372:CDS
CATCGTCTAGATTGGTCCAG+CGG	0.650779	3.1:-2696909	None:intergenic
GATGAGGAAGGCAGACGCCG+AGG	0.652733	3.1:+2697097	MS.gene009372:CDS
CAAATATGTATGCTGCTATG+AGG	0.656265	3.1:+2696802	MS.gene009372:CDS
TGTGGAAAGAACCATCAAAG+AGG	0.689890	3.1:+2696956	MS.gene009372:CDS
ATGCTATCGAATGATTGGAG+GGG	0.721195	3.1:+2696929	MS.gene009372:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAGACTAAATATAAAATC+CGG	+	chr3.1:2697075-2697094	MS.gene009372:CDS	20.0%
!	CCAAATTTACACCAAAATAA+CGG	+	chr3.1:2696836-2696855	MS.gene009372:CDS	25.0%
	AAATAAAGAAGGACTTGAAG+AGG	+	chr3.1:2696998-2697017	MS.gene009372:CDS	30.0%
	AGACATTGGAAAAGAAGAAA+AGG	+	chr3.1:2697039-2697058	MS.gene009372:CDS	30.0%
	CTAAATATAAAATCCGGATG+AGG	+	chr3.1:2697081-2697100	MS.gene009372:CDS	30.0%
	GACATTGGAAAAGAAGAAAA+GGG	+	chr3.1:2697040-2697059	MS.gene009372:CDS	30.0%
!	CAAAATAACGGAACTTGAAA+TGG	+	chr3.1:2696848-2696867	MS.gene009372:CDS	30.0%
!!	TTTTGGTGTAAATTTGGTTG+AGG	-	chr3.1:2696833-2696852	None:intergenic	30.0%
!!!	CATTTCAAGTTCCGTTATTT+TGG	-	chr3.1:2696850-2696869	None:intergenic	30.0%
!!!	CCGTTATTTTGGTGTAAATT+TGG	-	chr3.1:2696839-2696858	None:intergenic	30.0%
	ATATAAAATCCGGATGAGGA+AGG	+	chr3.1:2697085-2697104	MS.gene009372:CDS	35.0%
	CAAATATGTATGCTGCTATG+AGG	+	chr3.1:2696802-2696821	MS.gene009372:CDS	35.0%
	GAATGATGAATCTGGTAAGA+AGG	+	chr3.1:2697121-2697140	MS.gene009372:CDS	35.0%
	ACCAGATTCATCATTCACCT+CGG	-	chr3.1:2697117-2697136	None:intergenic	40.0%
	AGACGATGCTATCGAATGAT+TGG	+	chr3.1:2696924-2696943	MS.gene009372:CDS	40.0%
	ATGCTATCGAATGATTGGAG+GGG	+	chr3.1:2696929-2696948	MS.gene009372:CDS	40.0%
	GATGAATCTGGTAAGAAGGA+AGG	+	chr3.1:2697125-2697144	MS.gene009372:CDS	40.0%
	GATGCTATCGAATGATTGGA+GGG	+	chr3.1:2696928-2696947	MS.gene009372:CDS	40.0%
	GCAACTGCTTGTTCATTTAC+TGG	-	chr3.1:2696783-2696802	None:intergenic	40.0%
	TGTGGAAAGAACCATCAAAG+AGG	+	chr3.1:2696956-2696975	MS.gene009372:CDS	40.0%
	TTCGATAGCATCGTCTAGAT+TGG	-	chr3.1:2696920-2696939	None:intergenic	40.0%
	AAGAAGGAAGGTTCAGCTCA+AGG	+	chr3.1:2697137-2697156	MS.gene009372:CDS	45.0%
	CGATGCTATCGAATGATTGG+AGG	+	chr3.1:2696927-2696946	MS.gene009372:CDS	45.0%
	GCTGTGCAGCGAAATAAAGA+AGG	+	chr3.1:2696987-2697006	MS.gene009372:CDS	45.0%
!	TCTTTATTTCGCTGCACAGC+AGG	-	chr3.1:2696987-2697006	None:intergenic	45.0%
!!	TCAGCTCAAGGAGTACTTGT+GGG	+	chr3.1:2697149-2697168	MS.gene009372:CDS	45.0%
!!	TTCAGCTCAAGGAGTACTTG+TGG	+	chr3.1:2697148-2697167	MS.gene009372:CDS	45.0%
	AGCAGGCAAGACCTCTTTGA+TGG	-	chr3.1:2696970-2696989	None:intergenic	50.0%
	GACTTGAAGAGGTTGTTGCG+AGG	+	chr3.1:2697009-2697028	MS.gene009372:CDS	50.0%
	GCCGAGGTGAATGATGAATC+TGG	+	chr3.1:2697113-2697132	MS.gene009372:CDS	50.0%
	TAGTAAGGCCGAAGATCGCA+AGG	+	chr3.1:2696755-2696774	MS.gene009372:CDS	50.0%
	TCTTAATGCCATTCAGCCGC+TGG	+	chr3.1:2696893-2696912	MS.gene009372:CDS	50.0%
	TGCGAGGCTTAACGAGACAT+TGG	+	chr3.1:2697025-2697044	MS.gene009372:CDS	50.0%
!	CATCGTCTAGATTGGTCCAG+CGG	-	chr3.1:2696912-2696931	None:intergenic	50.0%
!!	AATGATTGGAGGGGTGCTTG+TGG	+	chr3.1:2696938-2696957	MS.gene009372:CDS	50.0%
!!	TACTGGCTCCTTGCGATCTT+CGG	-	chr3.1:2696766-2696785	None:intergenic	50.0%
!	AGATTGGTCCAGCGGCTGAA+TGG	-	chr3.1:2696904-2696923	None:intergenic	55.0%
	GGAAGTCAGCGAGCACTCGT+TGG	+	chr3.1:2696869-2696888	MS.gene009372:CDS	60.0%
!!	AGGAGTACTTGTGGGCCCTG+CGG	+	chr3.1:2697157-2697176	MS.gene009372:CDS	60.0%
	CGGCGTCTGCCTTCCTCATC+CGG	-	chr3.1:2697097-2697116	None:intergenic	65.0%
	GATGAGGAAGGCAGACGCCG+AGG	+	chr3.1:2697097-2697116	MS.gene009372:CDS	65.0%
!	GGAGTACTTGTGGGCCCTGC+GGG	+	chr3.1:2697158-2697177	MS.gene009372:CDS	65.0%
	GTACTTGTGGGCCCTGCGGG+TGG	+	chr3.1:2697161-2697180	MS.gene009372:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	2696750	2697193	2696750	ID=MS.gene009372
chr3.1	mRNA	2696750	2697193	2696750	ID=MS.gene009372.t1;Parent=MS.gene009372
chr3.1	exon	2696750	2697193	2696750	ID=MS.gene009372.t1.exon1;Parent=MS.gene009372.t1
chr3.1	CDS	2696750	2697193	2696750	ID=cds.MS.gene009372.t1;Parent=MS.gene009372.t1

Gene Sequence

>MS.gene009372

ATGGCTAGTAAGGCCGAAGATCGCAAGGAGCCAGTAAATGAACAAGCAGTTGCAAATATGTATGCTGCTATGAGGTCAGACCTCAACCAAATTTACACCAAAATAACGGAACTTGAAATGGAAGTCAGCGAGCACTCGTTGGTTCTTAATGCCATTCAGCCGCTGGACCAATCTAGACGATGCTATCGAATGATTGGAGGGGTGCTTGTGGAAAGAACCATCAAAGAGGTCTTGCCTGCTGTGCAGCGAAATAAAGAAGGACTTGAAGAGGTTGTTGCGAGGCTTAACGAGACATTGGAAAAGAAGAAAAGGGAAATTGCTGAGTTTGAGACTAAATATAAAATCCGGATGAGGAAGGCAGACGCCGAGGTGAATGATGAATCTGGTAAGAAGGAAGGTTCAGCTCAAGGAGTACTTGTGGGCCCTGCGGGTGGCAGCGAATGA

Protein sequence

>MS.gene009372.t1

MASKAEDRKEPVNEQAVANMYAAMRSDLNQIYTKITELEMEVSEHSLVLNAIQPLDQSRRCYRMIGGVLVERTIKEVLPAVQRNKEGLEEVVARLNETLEKKKREIAEFETKYKIRMRKADAEVNDESGKKEGSAQGVLVGPAGGSE