AlfalfaGEDB | MS.gene51515

M. sativa cultivar XinJiangDaYe / MS.gene51515

KEGG: K09549
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF01920.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51515.t1	XP_003598099.1	100	147	0	0	1	147	1	147	7.60E-70	273.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51515.t1	Q9LJ98	70.7	150	39	3	1	147	1	148	3.8e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51515.t1	G7J0A7	100.0	147	0	0	1	147	1	147	5.5e-70	273.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene51515	0.816202	6.24E-52	-1.69E-46
MS.gene050114	MS.gene51515	0.825845	3.75E-54	-1.69E-46
MS.gene050317	MS.gene51515	0.83514	1.99E-56	-1.69E-46
MS.gene050838	MS.gene51515	0.805423	1.34E-49	-1.69E-46
MS.gene051245	MS.gene51515	0.809143	2.19E-50	-1.69E-46
MS.gene051748	MS.gene51515	0.820702	5.97E-53	-1.69E-46
MS.gene051756	MS.gene51515	0.83929	1.73E-57	-1.69E-46
MS.gene051903	MS.gene51515	0.853004	3.18E-61	-1.69E-46
MS.gene052527	MS.gene51515	0.803298	3.73E-49	-1.69E-46
MS.gene05380	MS.gene51515	0.801904	7.22E-49	-1.69E-46
MS.gene053952	MS.gene51515	0.828541	8.49E-55	-1.69E-46
MS.gene055424	MS.gene51515	0.809848	1.54E-50	-1.69E-46
MS.gene057057	MS.gene51515	0.802123	6.51E-49	-1.69E-46
MS.gene057094	MS.gene51515	0.863244	2.86E-64	-1.69E-46
MS.gene057140	MS.gene51515	0.817535	3.14E-52	-1.69E-46
MS.gene057234	MS.gene51515	0.828525	8.57E-55	-1.69E-46
MS.gene057452	MS.gene51515	-0.802774	4.78E-49	-1.69E-46
MS.gene058182	MS.gene51515	0.803602	3.22E-49	-1.69E-46
MS.gene058238	MS.gene51515	0.849331	3.46E-60	-1.69E-46
MS.gene058654	MS.gene51515	0.80046	1.43E-48	-1.69E-46
MS.gene058906	MS.gene51515	0.820803	5.65E-53	-1.69E-46
MS.gene06001	MS.gene51515	0.81145	6.96E-51	-1.69E-46
MS.gene06011	MS.gene51515	0.801738	7.82E-49	-1.69E-46
MS.gene060297	MS.gene51515	0.820747	5.83E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene51515	MS.gene69089	PPI
MS.gene51515	MS.gene09540	PPI
MS.gene51515	MS.gene23694	PPI
MS.gene51515	MS.gene015202	PPI
MS.gene51515	MS.gene73093	PPI
MS.gene51515	MS.gene066551	PPI
MS.gene57177	MS.gene51515	PPI
MS.gene51515	MS.gene00146	PPI
MS.gene51515	MS.gene74586	PPI
MS.gene42750	MS.gene51515	PPI
MS.gene035826	MS.gene51515	PPI
MS.gene51515	MS.gene41343	PPI
MS.gene000365	MS.gene51515	PPI
MS.gene51515	MS.gene28046	PPI
MS.gene65650	MS.gene51515	PPI
MS.gene51515	MS.gene53708	PPI
MS.gene51515	MS.gene57325	PPI
MS.gene041917	MS.gene51515	PPI
MS.gene51515	MS.gene20252	PPI
MS.gene014578	MS.gene51515	PPI
MS.gene035828	MS.gene51515	PPI
MS.gene51515	MS.gene043643	PPI
MS.gene51515	MS.gene067039	PPI
MS.gene003245	MS.gene51515	PPI
MS.gene51515	MS.gene064353	PPI
MS.gene058013	MS.gene51515	PPI
MS.gene51515	MS.gene02047	PPI
MS.gene51515	MS.gene09543	PPI
MS.gene99872	MS.gene51515	PPI
MS.gene51515	MS.gene03171	PPI
MS.gene51515	MS.gene98781	PPI
MS.gene51515	MS.gene57178	PPI
MS.gene51515	MS.gene79100	PPI
MS.gene51515	MS.gene005125	PPI
MS.gene51515	MS.gene066566	PPI
MS.gene51515	MS.gene071261	PPI
MS.gene51515	MS.gene058245	PPI
MS.gene51515	MS.gene037362	PPI
MS.gene51515	MS.gene011754	PPI
MS.gene030840	MS.gene51515	PPI
MS.gene015206	MS.gene51515	PPI
MS.gene51515	MS.gene045141	PPI
MS.gene51515	MS.gene047578	PPI
MS.gene51515	MS.gene75567	PPI
MS.gene51515	MS.gene23930	PPI
MS.gene51515	MS.gene002442	PPI
MS.gene51515	MS.gene79126	PPI
MS.gene51515	MS.gene043595	PPI
MS.gene51515	MS.gene69947	PPI
MS.gene51515	MS.gene20253	PPI
MS.gene51515	MS.gene44510	PPI
MS.gene51515	MS.gene061811	PPI
MS.gene51515	MS.gene78457	PPI
MS.gene51515	MS.gene015213	PPI
MS.gene51515	MS.gene064351	PPI
MS.gene51515	MS.gene050877	PPI
MS.gene43992	MS.gene51515	PPI
MS.gene51515	MS.gene045765	PPI
MS.gene51515	MS.gene01284	PPI
MS.gene51515	MS.gene003428	PPI
MS.gene51515	MS.gene043593	PPI
MS.gene51515	MS.gene045227	PPI
MS.gene51515	MS.gene40225	PPI
MS.gene51515	MS.gene066877	PPI
MS.gene51515	MS.gene01697	PPI
MS.gene51515	MS.gene78156	PPI
MS.gene51515	MS.gene20245	PPI
MS.gene51515	MS.gene066875	PPI
MS.gene51515	MS.gene068943	PPI
MS.gene51515	MS.gene051698	PPI
MS.gene51515	MS.gene01091	PPI
MS.gene51515	MS.gene043645	PPI
MS.gene51515	MS.gene029211	PPI
MS.gene51515	MS.gene02265	PPI
MS.gene51515	MS.gene75501	PPI
MS.gene066552	MS.gene51515	PPI
MS.gene51515	MS.gene93131	PPI
MS.gene51515	MS.gene015212	PPI
MS.gene51515	MS.gene035825	PPI
MS.gene51515	MS.gene36102	PPI
MS.gene51515	MS.gene066876	PPI
MS.gene51515	MS.gene84699	PPI
MS.gene51515	MS.gene002306	PPI
MS.gene51515	MS.gene77306	PPI
MS.gene09552	MS.gene51515	PPI
MS.gene51515	MS.gene43369	PPI
MS.gene51515	MS.gene09551	PPI
MS.gene51515	MS.gene01425	PPI
MS.gene51515	MS.gene67725	PPI
MS.gene51515	MS.gene55980	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51515.t1	MTR_3g007250	100.000	147	0	0	1	147	1	147	4.41e-105	296

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51515.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201
MS.gene51515.t1	AT3G22480	70.667	150	39	3	1	147	1	148	2.03e-67	201

Find 44 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTCAAGTTCCGTTATTT+TGG	0.192430	3.4:-2923488	None:intergenic
GCAACTGCTTGTTCATTTAC+TGG	0.248625	3.4:-2923421	None:intergenic
TACTGGCTCCTTGCGATCTT+CGG	0.331035	3.4:-2923404	None:intergenic
CAAAATAACGGAACTTGAAA+TGG	0.346568	3.4:+2923489	MS.gene51515:CDS
AGCAGGCAGGACCTCTTTGA+TGG	0.408930	3.4:-2923608	None:intergenic
AGACATTGGAAAAGAAGAAA+AGG	0.418775	3.4:+2923680	MS.gene51515:CDS
TTGAGACTAAATATAAAATC+AGG	0.420368	3.4:+2923716	MS.gene51515:CDS
GGAGTACTTGTGGGCCCTGC+GGG	0.425415	3.4:+2923799	MS.gene51515:CDS
TCTTTATTCCGCTGCACAGC+AGG	0.438536	3.4:-2923625	None:intergenic
AGACGATGCTATCGAATGAT+TGG	0.440454	3.4:+2923565	MS.gene51515:CDS
TTCGATAGCATCGTCTAGAT+TGG	0.458077	3.4:-2923558	None:intergenic
GACATTGGAAAAGAAGAAAA+GGG	0.500685	3.4:+2923681	MS.gene51515:CDS
GTGTAGAATCATGGCTAGTA+AGG	0.508954	3.4:+2923381	None:intergenic
GCTGAGGTGAACGATGAATC+TGG	0.515292	3.4:+2923754	MS.gene51515:CDS
CCAAATTTACACCAAAATAA+CGG	0.527386	3.4:+2923477	MS.gene51515:CDS
AGATTGGTCTAGCGGCTGAA+TGG	0.531922	3.4:-2923542	None:intergenic
TGTCATTCGCTACCACCCGC+AGG	0.539315	3.4:-2923814	None:intergenic
TATTCCGCTGCACAGCAGGC+AGG	0.540606	3.4:-2923621	None:intergenic
TGCAAGGCTTAACGAGACAT+TGG	0.541862	3.4:+2923666	MS.gene51515:CDS
GTACTTGTGGGCCCTGCGGG+TGG	0.543152	3.4:+2923802	MS.gene51515:CDS
GAACGATGAATCTGGTAAGA+AGG	0.544140	3.4:+2923762	MS.gene51515:CDS
TCAGCTCAAGGAGTACTTGT+GGG	0.554339	3.4:+2923790	MS.gene51515:CDS
AAGAAGGAAGGTTCAGCTCA+AGG	0.558366	3.4:+2923778	MS.gene51515:CDS
GCTGTGCAGCGGAATAAAGA+AGG	0.563818	3.4:+2923628	MS.gene51515:CDS
CGATGCTATCGAATGATTGG+AGG	0.563926	3.4:+2923568	MS.gene51515:CDS
TTCATCGTTCACCTCAGCAT+CGG	0.575473	3.4:-2923749	None:intergenic
GATGAATCTGGTAAGAAGGA+AGG	0.587326	3.4:+2923766	MS.gene51515:CDS
CTAAATATAAAATCAGGATG+AGG	0.587775	3.4:+2923722	MS.gene51515:CDS
GATGAGGAAGGCCGATGCTG+AGG	0.588771	3.4:+2923738	MS.gene51515:CDS
AGGTCCTGCCTGCTGTGCAG+CGG	0.589956	3.4:+2923617	MS.gene51515:CDS
GACTTGAAGAGGTTGTTGCA+AGG	0.593695	3.4:+2923650	MS.gene51515:CDS
GTCATTCGCTACCACCCGCA+GGG	0.594608	3.4:-2923813	None:intergenic
ATATAAAATCAGGATGAGGA+AGG	0.596264	3.4:+2923726	MS.gene51515:CDS
TAGTAAGGCCGAAGATCGCA+AGG	0.597879	3.4:+2923396	MS.gene51515:CDS
AGGAGTACTTGTGGGCCCTG+CGG	0.600783	3.4:+2923798	MS.gene51515:CDS
TTCAGCTCAAGGAGTACTTG+TGG	0.603923	3.4:+2923789	MS.gene51515:CDS
AATGATTGGAGGGGTGCTTG+TGG	0.605277	3.4:+2923579	MS.gene51515:CDS
GATGCTATCGAATGATTGGA+GGG	0.617244	3.4:+2923569	MS.gene51515:CDS
GGAAGTCAGTGAGCACTCGT+TGG	0.621570	3.4:+2923510	MS.gene51515:CDS
CAAATATGTATGCAGCTATG+AGG	0.631644	3.4:+2923443	MS.gene51515:CDS
CATCGTCTAGATTGGTCTAG+CGG	0.643268	3.4:-2923550	None:intergenic
GAATAAAGAAGGACTTGAAG+AGG	0.645797	3.4:+2923639	MS.gene51515:CDS
TGTGGAAAGAACCATCAAAG+AGG	0.692372	3.4:+2923597	MS.gene51515:CDS
ATGCTATCGAATGATTGGAG+GGG	0.721195	3.4:+2923570	MS.gene51515:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAGACTAAATATAAAATC+AGG	+	chr3.4:2923716-2923735	MS.gene51515:CDS	20.0%
!	CCAAATTTACACCAAAATAA+CGG	+	chr3.4:2923477-2923496	MS.gene51515:CDS	25.0%
!	CTAAATATAAAATCAGGATG+AGG	+	chr3.4:2923722-2923741	MS.gene51515:CDS	25.0%
	AGACATTGGAAAAGAAGAAA+AGG	+	chr3.4:2923680-2923699	MS.gene51515:CDS	30.0%
	ATATAAAATCAGGATGAGGA+AGG	+	chr3.4:2923726-2923745	MS.gene51515:CDS	30.0%
	GACATTGGAAAAGAAGAAAA+GGG	+	chr3.4:2923681-2923700	MS.gene51515:CDS	30.0%
!	CAAAATAACGGAACTTGAAA+TGG	+	chr3.4:2923489-2923508	MS.gene51515:CDS	30.0%
!!	TTTTGGTGTAAATTTGGTTG+AGG	-	chr3.4:2923474-2923493	None:intergenic	30.0%
!!!	CATTTCAAGTTCCGTTATTT+TGG	-	chr3.4:2923491-2923510	None:intergenic	30.0%
!!!	CCGTTATTTTGGTGTAAATT+TGG	-	chr3.4:2923480-2923499	None:intergenic	30.0%
	CAAATATGTATGCAGCTATG+AGG	+	chr3.4:2923443-2923462	MS.gene51515:CDS	35.0%
	GAATAAAGAAGGACTTGAAG+AGG	+	chr3.4:2923639-2923658	MS.gene51515:CDS	35.0%
	AGACGATGCTATCGAATGAT+TGG	+	chr3.4:2923565-2923584	MS.gene51515:CDS	40.0%
	ATGCTATCGAATGATTGGAG+GGG	+	chr3.4:2923570-2923589	MS.gene51515:CDS	40.0%
	GAACGATGAATCTGGTAAGA+AGG	+	chr3.4:2923762-2923781	MS.gene51515:CDS	40.0%
	GATGAATCTGGTAAGAAGGA+AGG	+	chr3.4:2923766-2923785	MS.gene51515:CDS	40.0%
	GATGCTATCGAATGATTGGA+GGG	+	chr3.4:2923569-2923588	MS.gene51515:CDS	40.0%
	GCAACTGCTTGTTCATTTAC+TGG	-	chr3.4:2923424-2923443	None:intergenic	40.0%
	TGTGGAAAGAACCATCAAAG+AGG	+	chr3.4:2923597-2923616	MS.gene51515:CDS	40.0%
	TTCGATAGCATCGTCTAGAT+TGG	-	chr3.4:2923561-2923580	None:intergenic	40.0%
	AAGAAGGAAGGTTCAGCTCA+AGG	+	chr3.4:2923778-2923797	MS.gene51515:CDS	45.0%
	CGATGCTATCGAATGATTGG+AGG	+	chr3.4:2923568-2923587	MS.gene51515:CDS	45.0%
	GACTTGAAGAGGTTGTTGCA+AGG	+	chr3.4:2923650-2923669	MS.gene51515:CDS	45.0%
	TGCAAGGCTTAACGAGACAT+TGG	+	chr3.4:2923666-2923685	MS.gene51515:CDS	45.0%
	TTCATCGTTCACCTCAGCAT+CGG	-	chr3.4:2923752-2923771	None:intergenic	45.0%
!!	CATCGTCTAGATTGGTCTAG+CGG	-	chr3.4:2923553-2923572	None:intergenic	45.0%
!!	TCAGCTCAAGGAGTACTTGT+GGG	+	chr3.4:2923790-2923809	MS.gene51515:CDS	45.0%
!!	TTCAGCTCAAGGAGTACTTG+TGG	+	chr3.4:2923789-2923808	MS.gene51515:CDS	45.0%
	GCTGAGGTGAACGATGAATC+TGG	+	chr3.4:2923754-2923773	MS.gene51515:CDS	50.0%
	GCTGTGCAGCGGAATAAAGA+AGG	+	chr3.4:2923628-2923647	MS.gene51515:CDS	50.0%
	TAGTAAGGCCGAAGATCGCA+AGG	+	chr3.4:2923396-2923415	MS.gene51515:CDS	50.0%
	TCTTTATTCCGCTGCACAGC+AGG	-	chr3.4:2923628-2923647	None:intergenic	50.0%
!!	AATGATTGGAGGGGTGCTTG+TGG	+	chr3.4:2923579-2923598	MS.gene51515:CDS	50.0%
!!	AGATTGGTCTAGCGGCTGAA+TGG	-	chr3.4:2923545-2923564	None:intergenic	50.0%
!!	TACTGGCTCCTTGCGATCTT+CGG	-	chr3.4:2923407-2923426	None:intergenic	50.0%
	AGCAGGCAGGACCTCTTTGA+TGG	-	chr3.4:2923611-2923630	None:intergenic	55.0%
	GGAAGTCAGTGAGCACTCGT+TGG	+	chr3.4:2923510-2923529	MS.gene51515:CDS	55.0%
	GATGAGGAAGGCCGATGCTG+AGG	+	chr3.4:2923738-2923757	MS.gene51515:CDS	60.0%
	TATTCCGCTGCACAGCAGGC+AGG	-	chr3.4:2923624-2923643	None:intergenic	60.0%
!!	AGGAGTACTTGTGGGCCCTG+CGG	+	chr3.4:2923798-2923817	MS.gene51515:CDS	60.0%
	AGGTCCTGCCTGCTGTGCAG+CGG	+	chr3.4:2923617-2923636	MS.gene51515:CDS	65.0%
!	GGAGTACTTGTGGGCCCTGC+GGG	+	chr3.4:2923799-2923818	MS.gene51515:CDS	65.0%
	GTACTTGTGGGCCCTGCGGG+TGG	+	chr3.4:2923802-2923821	MS.gene51515:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	2923391	2923834	2923391	ID=MS.gene51515
chr3.4	mRNA	2923391	2923834	2923391	ID=MS.gene51515.t1;Parent=MS.gene51515
chr3.4	exon	2923391	2923834	2923391	ID=MS.gene51515.t1.exon1;Parent=MS.gene51515.t1
chr3.4	CDS	2923391	2923834	2923391	ID=cds.MS.gene51515.t1;Parent=MS.gene51515.t1

Gene Sequence

>MS.gene51515

ATGGCTAGTAAGGCCGAAGATCGCAAGGAGCCAGTAAATGAACAAGCAGTTGCAAATATGTATGCAGCTATGAGGTCAGACCTCAACCAAATTTACACCAAAATAACGGAACTTGAAATGGAAGTCAGTGAGCACTCGTTGGTTCTTAATGCCATTCAGCCGCTAGACCAATCTAGACGATGCTATCGAATGATTGGAGGGGTGCTTGTGGAAAGAACCATCAAAGAGGTCCTGCCTGCTGTGCAGCGGAATAAAGAAGGACTTGAAGAGGTTGTTGCAAGGCTTAACGAGACATTGGAAAAGAAGAAAAGGGAAATTGCTGAGTTTGAGACTAAATATAAAATCAGGATGAGGAAGGCCGATGCTGAGGTGAACGATGAATCTGGTAAGAAGGAAGGTTCAGCTCAAGGAGTACTTGTGGGCCCTGCGGGTGGTAGCGAATGA

Protein sequence

>MS.gene51515.t1

MASKAEDRKEPVNEQAVANMYAAMRSDLNQIYTKITELEMEVSEHSLVLNAIQPLDQSRRCYRMIGGVLVERTIKEVLPAVQRNKEGLEEVVARLNETLEKKKREIAEFETKYKIRMRKADAEVNDESGKKEGSAQGVLVGPAGGSE