Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068727.t1 | RHN75073.1 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 2.40E-34 | 154.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068727.t1 | A0A072VB89 | 91.6 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 1.7e-34 | 154.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene068727 | 0.807058 | 6.08E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene068727 | 0.826931 | 2.07E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene068727 | 0.812094 | 5.04E-51 | -1.69E-46 |
| MS.gene050237 | MS.gene068727 | 0.822163 | 2.74E-53 | -1.69E-46 |
| MS.gene050330 | MS.gene068727 | -0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene068727 | 0.832235 | 1.06E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene068727 | 0.828535 | 8.52E-55 | -1.69E-46 |
| MS.gene050721 | MS.gene068727 | 0.828727 | 7.65E-55 | -1.69E-46 |
| MS.gene050724 | MS.gene068727 | 0.807589 | 4.70E-50 | -1.69E-46 |
| MS.gene050824 | MS.gene068727 | 0.801446 | 8.97E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene068727 | 0.811932 | 5.47E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene068727 | 0.809421 | 1.91E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene068727 | -0.807194 | 5.69E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene068727 | 0.801512 | 8.70E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene068727 | 0.819781 | 9.70E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene068727 | 0.800017 | 1.76E-48 | -1.69E-46 |
| MS.gene053054 | MS.gene068727 | -0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene053085 | MS.gene068727 | 0.805731 | 1.16E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene068727 | 0.804325 | 2.28E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene068727 | -0.80034 | 1.51E-48 | -1.69E-46 |
| MS.gene054468 | MS.gene068727 | 0.801558 | 8.51E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene068727 | 0.835818 | 1.34E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene068727 | 0.802894 | 4.52E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene068727 | 0.832059 | 1.17E-55 | -1.69E-46 |
| MS.gene057060 | MS.gene068727 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene057414 | MS.gene068727 | 0.800575 | 1.35E-48 | -1.69E-46 |
| MS.gene057980 | MS.gene068727 | 0.805765 | 1.14E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene068727 | 0.801675 | 8.05E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene068727 | 0.811103 | 8.28E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene068727 | 0.808828 | 2.56E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene068727 | 0.804906 | 1.72E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene068727 | 0.829445 | 5.13E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene068727 | 0.825018 | 5.89E-54 | -1.69E-46 |
| MS.gene060521 | MS.gene068727 | 0.820215 | 7.72E-53 | -1.69E-46 |
| MS.gene060553 | MS.gene068727 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene068727 | 0.837379 | 5.38E-57 | -1.69E-46 |
| MS.gene060683 | MS.gene068727 | 0.822809 | 1.94E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene068727 | 0.82301 | 1.74E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene068727 | -0.815136 | 1.08E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068727.t1 | MTR_2g079000 | 91.566 | 83 | 5 | 1 | 7 | 87 | 1 | 83 | 5.01e-42 | 137 |
| MS.gene068727.t1 | MTR_5g012820 | 65.517 | 87 | 25 | 3 | 3 | 87 | 7 | 90 | 5.56e-25 | 95.9 |
| MS.gene068727.t1 | MTR_5g012820 | 66.667 | 81 | 24 | 2 | 7 | 87 | 3 | 80 | 1.16e-23 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068727.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 24 | 81 | 3.40e-13 | 63.5 |
| MS.gene068727.t1 | AT5G05210 | 57.627 | 59 | 24 | 1 | 29 | 87 | 16 | 73 | 3.87e-13 | 63.5 |
Find 18 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACGAGAAGCCATGGTTTC+AGG | 0.217653 | 2.3:+18914802 | MS.gene068727:CDS |
| CTGAAGGGTGTTGCAGATAT+TGG | 0.394867 | 2.3:+18914680 | MS.gene068727:CDS |
| GCTGTTACAATGAGAGATTC+CGG | 0.414625 | 2.3:+18914635 | None:intergenic |
| GTTGGTAGATAAAACTTAGC+TGG | 0.463279 | 2.3:-18914773 | None:intergenic |
| AACGAGAAGCCATGGTTTCA+GGG | 0.477522 | 2.3:+18914803 | MS.gene068727:CDS |
| TATTGGTGCTTGTCAAGACT+TGG | 0.520276 | 2.3:+18914697 | MS.gene068727:CDS |
| GTTAGGGTCTGAACTCTAAA+CGG | 0.521104 | 2.3:-18914895 | None:intergenic |
| GGCTTCTCGTTATCATCAGT+TGG | 0.521411 | 2.3:-18914791 | None:intergenic |
| AATGAGAGATTCCGGAGTGA+TGG | 0.528245 | 2.3:+18914643 | None:intergenic |
| TTGCTTAGACCCTGAAACCA+TGG | 0.533554 | 2.3:-18914812 | None:intergenic |
| GGAGATTAAGAAGAAGCTGA+AGG | 0.558266 | 2.3:+18914664 | MS.gene068727:CDS |
| AAGCAAGGGTGCAAAAGCTA+AGG | 0.564446 | 2.3:+18914829 | MS.gene068727:CDS |
| GAGATTAAGAAGAAGCTGAA+GGG | 0.579276 | 2.3:+18914665 | MS.gene068727:CDS |
| TAGACAATATTATGAAGTCT+CGG | 0.586642 | 2.3:+18914867 | MS.gene068727:CDS |
| ATGGTTTCAGGGTCTAAGCA+AGG | 0.591717 | 2.3:+18914814 | MS.gene068727:CDS |
| CTTCTTAATCTCCATCACTC+CGG | 0.599988 | 2.3:-18914654 | None:intergenic |
| CTGATGATAACGAGAAGCCA+TGG | 0.624704 | 2.3:+18914795 | MS.gene068727:CDS |
| TGGTTTCAGGGTCTAAGCAA+GGG | 0.633440 | 2.3:+18914815 | MS.gene068727:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAATTTGTCGAAAAAAT+GGG | - | chr2.3:18914744-18914763 | None:intergenic | 25.0% |
| ! | TAGACAATATTATGAAGTCT+CGG | + | chr2.3:18914867-18914886 | MS.gene068727:CDS | 25.0% |
| CGATCAATTTGTCGAAAAAA+TGG | - | chr2.3:18914745-18914764 | None:intergenic | 30.0% | |
| GAGATTAAGAAGAAGCTGAA+GGG | + | chr2.3:18914665-18914684 | MS.gene068727:CDS | 35.0% | |
| ! | GTTGGTAGATAAAACTTAGC+TGG | - | chr2.3:18914776-18914795 | None:intergenic | 35.0% |
| CTTCTTAATCTCCATCACTC+CGG | - | chr2.3:18914657-18914676 | None:intergenic | 40.0% | |
| GGAGATTAAGAAGAAGCTGA+AGG | + | chr2.3:18914664-18914683 | MS.gene068727:CDS | 40.0% | |
| ! | TATTGGTGCTTGTCAAGACT+TGG | + | chr2.3:18914697-18914716 | MS.gene068727:CDS | 40.0% |
| AACGAGAAGCCATGGTTTCA+GGG | + | chr2.3:18914803-18914822 | MS.gene068727:CDS | 45.0% | |
| ATGGTTTCAGGGTCTAAGCA+AGG | + | chr2.3:18914814-18914833 | MS.gene068727:CDS | 45.0% | |
| GGCTTCTCGTTATCATCAGT+TGG | - | chr2.3:18914794-18914813 | None:intergenic | 45.0% | |
| TAACGAGAAGCCATGGTTTC+AGG | + | chr2.3:18914802-18914821 | MS.gene068727:CDS | 45.0% | |
| TGGTTTCAGGGTCTAAGCAA+GGG | + | chr2.3:18914815-18914834 | MS.gene068727:CDS | 45.0% | |
| TTGCTTAGACCCTGAAACCA+TGG | - | chr2.3:18914815-18914834 | None:intergenic | 45.0% | |
| ! | AAGCAAGGGTGCAAAAGCTA+AGG | + | chr2.3:18914829-18914848 | MS.gene068727:CDS | 45.0% |
| ! | CTGATGATAACGAGAAGCCA+TGG | + | chr2.3:18914795-18914814 | MS.gene068727:CDS | 45.0% |
| !! | CTGAAGGGTGTTGCAGATAT+TGG | + | chr2.3:18914680-18914699 | MS.gene068727:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 18914644 | 18914916 | 18914644 | ID=MS.gene068727 |
| chr2.3 | mRNA | 18914644 | 18914916 | 18914644 | ID=MS.gene068727.t1;Parent=MS.gene068727 |
| chr2.3 | exon | 18914644 | 18914916 | 18914644 | ID=MS.gene068727.t1.exon1;Parent=MS.gene068727.t1 |
| chr2.3 | CDS | 18914644 | 18914916 | 18914644 | ID=cds.MS.gene068727.t1;Parent=MS.gene068727.t1 |
| Gene Sequence |
| Protein sequence |
