AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06890.t1 MTR_3g091820 74.315 292 6 5 1 225 17 306 2.93e-133 377
MS.gene06890.t1 MTR_8g027295 36.364 286 104 15 1 225 1 269 5.07e-33 120
MS.gene06890.t1 MTR_1g077600 33.721 258 89 14 14 216 30 260 1.58e-19 84.7
MS.gene06890.t1 MTR_1g077600 33.721 258 89 14 14 216 30 260 1.83e-19 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06890.t1 AT3G61850 29.643 280 113 13 13 225 50 312 2.25e-22 93.2
MS.gene06890.t1 AT3G61850 29.643 280 113 13 13 225 62 324 4.06e-22 92.8
MS.gene06890.t1 AT3G61850 31.418 261 105 14 13 225 50 284 9.35e-21 88.6
MS.gene06890.t1 AT3G61850 31.418 261 105 14 13 225 50 284 9.35e-21 88.6
MS.gene06890.t1 AT3G61850 31.418 261 105 14 13 225 62 296 1.62e-20 87.8

Find 48 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTCCTTGATGGATCTT+AGG 0.169048 3.2:-77302869 None:intergenic
TTTAAGCTTGGGGTTAATTT+TGG 0.185562 3.2:+77303109 MS.gene06890:CDS
TGTTCAAACTCACCACTATT+TGG 0.210892 3.2:-77303283 None:intergenic
AAGGTGATTCTACTGGTTAT+TGG 0.303787 3.2:+77303323 MS.gene06890:CDS
GAAGGGTTTGAGAATGGTTA+TGG 0.323309 3.2:+77303136 MS.gene06890:CDS
GGTTAAGATCTTGTCCTTGA+TGG 0.347085 3.2:-77302877 None:intergenic
GCAAAACTTGTAGAAGGTAT+TGG 0.349440 3.2:+77302660 MS.gene06890:intron
GGTTCTGCTGGTGGTGGTGG+TGG 0.365919 3.2:+77303187 MS.gene06890:CDS
GAGGGTTCTGCTGGTGGTGG+TGG 0.372229 3.2:+77303184 MS.gene06890:CDS
CTTGTCCTTGATGGATCTTA+GGG 0.390118 3.2:-77302868 None:intergenic
TGAAAACTGCTTCTTCATCA+AGG 0.432756 3.2:+77303008 MS.gene06890:CDS
AAGCTCAATGAATTTAGATA+TGG 0.438747 3.2:-77302939 None:intergenic
AACTGATGATCATCTACAAC+AGG 0.454576 3.2:-77303235 None:intergenic
TTGTCTTGATCAGTTGTGTA+AGG 0.463233 3.2:-77302962 None:intergenic
GGTTTAGAAGGGTTTGAGAA+TGG 0.472180 3.2:+77303130 MS.gene06890:CDS
CTCTCTAACATAGAAGGTGT+TGG 0.476502 3.2:-77302527 None:intergenic
GATTCTACTGGTTATTGGAA+TGG 0.482077 3.2:+77303328 MS.gene06890:CDS
CTAGACTCAGACAAAGTCAT+AGG 0.485051 3.2:-77303049 None:intergenic
GAAAACTGCTTCTTCATCAA+GGG 0.488666 3.2:+77303009 MS.gene06890:CDS
CAGCAACAAATTCCAAATAG+TGG 0.488831 3.2:+77303271 MS.gene06890:CDS
TAATTATCCTCAGTTGTTTG+TGG 0.490761 3.2:-77302911 None:intergenic
CAAGAGGGTTCTGCTGGTGG+TGG 0.493771 3.2:+77303181 MS.gene06890:CDS
TTGTTTGTGGTGGATAAGCT+AGG 0.500159 3.2:-77302898 None:intergenic
AATGGTTATGGTGGCTTGCA+AGG 0.516585 3.2:+77303148 MS.gene06890:CDS
AATAATCAAGAGGGTTCTGC+TGG 0.524023 3.2:+77303175 MS.gene06890:CDS
TCTTTAAGAAATGTTCCTGT+AGG 0.524165 3.2:+77302695 MS.gene06890:CDS
GAATGTTCGGTGGAGGATCA+TGG 0.528349 3.2:+77303350 MS.gene06890:CDS
TTATCCTCAGTTGTTTGTGG+TGG 0.529976 3.2:-77302908 None:intergenic
AGACAAAACTACAAGTGCTA+TGG 0.533697 3.2:+77302979 MS.gene06890:CDS
AGAACTCAAGGTGATTCTAC+TGG 0.537114 3.2:+77303316 MS.gene06890:CDS
CATCAGCAAAATAGAACTCA+AGG 0.544205 3.2:+77303304 MS.gene06890:CDS
GGTTATTGGAATGGAATGTT+CGG 0.551153 3.2:+77303337 MS.gene06890:CDS
GGATTGGTTAAACAAATGGA+TGG 0.552908 3.2:+77302485 None:intergenic
TGGAATGGAATGTTCGGTGG+AGG 0.558324 3.2:+77303343 MS.gene06890:CDS
ATCAAGCTTTAAACTGTCCA+AGG 0.561852 3.2:+77302570 MS.gene06890:CDS
GGGTTTGAGAATGGTTATGG+TGG 0.564934 3.2:+77303139 MS.gene06890:CDS
AAAACTGCTTCTTCATCAAG+GGG 0.565168 3.2:+77303010 MS.gene06890:CDS
AGTGCAATGTATAATTCAAG+TGG 0.566445 3.2:+77303070 MS.gene06890:CDS
CAAAACCCTAAGATCCATCA+AGG 0.571674 3.2:+77302863 MS.gene06890:CDS
TATTGGAATGGAATGTTCGG+TGG 0.576421 3.2:+77303340 MS.gene06890:CDS
CAAGGGATTAATAATCAAGA+GGG 0.577580 3.2:+77303166 MS.gene06890:CDS
GCAAGGGATTAATAATCAAG+AGG 0.579146 3.2:+77303165 MS.gene06890:CDS
ATGGTTATGGTGGCTTGCAA+GGG 0.581200 3.2:+77303149 MS.gene06890:CDS
TGTAGAAGGTATTGGACTGA+AGG 0.583395 3.2:+77302668 MS.gene06890:intron
AATCAAGAGGGTTCTGCTGG+TGG 0.591602 3.2:+77303178 MS.gene06890:CDS
AGAAGGTATTGGACTGAAGG+TGG 0.592159 3.2:+77302671 MS.gene06890:intron
TTATCCACCACAAACAACTG+AGG 0.701982 3.2:+77302904 MS.gene06890:CDS
CTATGTTAGAGAGAAAAGCA+AGG 0.712069 3.2:+77302537 MS.gene06890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTGTTATTGATGA+TGG - chr3.2:77302788-77302807 None:intergenic 15.0%
! AAGCTCAATGAATTTAGATA+TGG - chr3.2:77302942-77302961 None:intergenic 25.0%
!!! TTTAAAGCTTGATCTTTTTG+TGG - chr3.2:77302563-77302582 None:intergenic 25.0%
!!! TTTTTTGTTATTGATGATGG+AGG - chr3.2:77302785-77302804 None:intergenic 25.0%
AGTGCAATGTATAATTCAAG+TGG + chr3.2:77303070-77303089 MS.gene06890:CDS 30.0%
CAAGGGATTAATAATCAAGA+GGG + chr3.2:77303166-77303185 MS.gene06890:CDS 30.0%
TAATTATCCTCAGTTGTTTG+TGG - chr3.2:77302914-77302933 None:intergenic 30.0%
! ACTTTTGCAAAACTTGTAGA+AGG + chr3.2:77302654-77302673 MS.gene06890:intron 30.0%
! CTTTGCAAGATTTTAAGCTT+GGG + chr3.2:77303098-77303117 MS.gene06890:CDS 30.0%
! TCTTTAAGAAATGTTCCTGT+AGG + chr3.2:77302695-77302714 MS.gene06890:CDS 30.0%
! TTTAAGCTTGGGGTTAATTT+TGG + chr3.2:77303109-77303128 MS.gene06890:CDS 30.0%
! TTTGCAAGATTTTAAGCTTG+GGG + chr3.2:77303099-77303118 MS.gene06890:CDS 30.0%
!!! GGGTTAATTTTGGTTTAGAA+GGG + chr3.2:77303119-77303138 MS.gene06890:CDS 30.0%
AAAACTGCTTCTTCATCAAG+GGG + chr3.2:77303010-77303029 MS.gene06890:CDS 35.0%
AACTGATGATCATCTACAAC+AGG - chr3.2:77303238-77303257 None:intergenic 35.0%
AGACAAAACTACAAGTGCTA+TGG + chr3.2:77302979-77302998 MS.gene06890:CDS 35.0%
CAGCAACAAATTCCAAATAG+TGG + chr3.2:77303271-77303290 MS.gene06890:CDS 35.0%
CATCAGCAAAATAGAACTCA+AGG + chr3.2:77303304-77303323 MS.gene06890:CDS 35.0%
CTATGTTAGAGAGAAAAGCA+AGG + chr3.2:77302537-77302556 MS.gene06890:CDS 35.0%
GAAAACTGCTTCTTCATCAA+GGG + chr3.2:77303009-77303028 MS.gene06890:CDS 35.0%
GCAAGGGATTAATAATCAAG+AGG + chr3.2:77303165-77303184 MS.gene06890:CDS 35.0%
GGTTATTGGAATGGAATGTT+CGG + chr3.2:77303337-77303356 MS.gene06890:CDS 35.0%
TGAAAACTGCTTCTTCATCA+AGG + chr3.2:77303008-77303027 MS.gene06890:CDS 35.0%
TGTTCAAACTCACCACTATT+TGG - chr3.2:77303286-77303305 None:intergenic 35.0%
TTAAGAAATGTTCCTGTAGG+TGG + chr3.2:77302698-77302717 MS.gene06890:intron 35.0%
TTGTCTTGATCAGTTGTGTA+AGG - chr3.2:77302965-77302984 None:intergenic 35.0%
! AAGGTGATTCTACTGGTTAT+TGG + chr3.2:77303323-77303342 MS.gene06890:CDS 35.0%
! GATTCTACTGGTTATTGGAA+TGG + chr3.2:77303328-77303347 MS.gene06890:CDS 35.0%
! GCAAAACTTGTAGAAGGTAT+TGG + chr3.2:77302660-77302679 MS.gene06890:intron 35.0%
! GCTTTGCAAGATTTTAAGCT+TGG + chr3.2:77303097-77303116 MS.gene06890:CDS 35.0%
! GCTTTTCTCTCTAACATAGA+AGG - chr3.2:77302536-77302555 None:intergenic 35.0%
!! ATCAAGCTTTAAACTGTCCA+AGG + chr3.2:77302570-77302589 MS.gene06890:CDS 35.0%
!!! CAAGTTTTGCAAAAGTACCT+TGG - chr3.2:77302650-77302669 None:intergenic 35.0%
!!! GGGGTTAATTTTGGTTTAGA+AGG + chr3.2:77303118-77303137 MS.gene06890:CDS 35.0%
AATAATCAAGAGGGTTCTGC+TGG + chr3.2:77303175-77303194 MS.gene06890:CDS 40.0%
AGAACTCAAGGTGATTCTAC+TGG + chr3.2:77303316-77303335 MS.gene06890:CDS 40.0%
CAAAACCCTAAGATCCATCA+AGG + chr3.2:77302863-77302882 MS.gene06890:CDS 40.0%
CTAGACTCAGACAAAGTCAT+AGG - chr3.2:77303052-77303071 None:intergenic 40.0%
GAAGGGTTTGAGAATGGTTA+TGG + chr3.2:77303136-77303155 MS.gene06890:CDS 40.0%
GGTTAAGATCTTGTCCTTGA+TGG - chr3.2:77302880-77302899 None:intergenic 40.0%
GGTTGTGATTGTGAGATTGA+AGG - chr3.2:77302764-77302783 None:intergenic 40.0%
GGTTTAGAAGGGTTTGAGAA+TGG + chr3.2:77303130-77303149 MS.gene06890:CDS 40.0%
GTGTTAGTTGAATTGCACCT+TGG - chr3.2:77302590-77302609 None:intergenic 40.0%
TATTGGAATGGAATGTTCGG+TGG + chr3.2:77303340-77303359 MS.gene06890:CDS 40.0%
TTATCCACCACAAACAACTG+AGG + chr3.2:77302904-77302923 MS.gene06890:CDS 40.0%
TTATCCTCAGTTGTTTGTGG+TGG - chr3.2:77302911-77302930 None:intergenic 40.0%
! CTCTCTAACATAGAAGGTGT+TGG - chr3.2:77302530-77302549 None:intergenic 40.0%
! CTTGTCCTTGATGGATCTTA+GGG - chr3.2:77302871-77302890 None:intergenic 40.0%
! GATTGAAGGTGATGATGATG+AGG - chr3.2:77302750-77302769 None:intergenic 40.0%
! TCTTGTCCTTGATGGATCTT+AGG - chr3.2:77302872-77302891 None:intergenic 40.0%
! TTTTGTGCTGTAGCAAGATC+AGG - chr3.2:77302821-77302840 None:intergenic 40.0%
!! AAAAGTACCTTGGTTGTGAG+AGG - chr3.2:77302640-77302659 None:intergenic 40.0%
!! TGTAGAAGGTATTGGACTGA+AGG + chr3.2:77302668-77302687 MS.gene06890:intron 40.0%
!! TTGTTTGTGGTGGATAAGCT+AGG - chr3.2:77302901-77302920 None:intergenic 40.0%
!!! GGTTTTGAGAAGCAGATTGA+GGG - chr3.2:77302850-77302869 None:intergenic 40.0%
ACTATAGCCTCTCACAACCA+AGG + chr3.2:77302630-77302649 MS.gene06890:intron 45.0%
AGAAATGTTCCTGTAGGTGG+TGG + chr3.2:77302701-77302720 MS.gene06890:intron 45.0%
GGGTTTGAGAATGGTTATGG+TGG + chr3.2:77303139-77303158 MS.gene06890:CDS 45.0%
TTTCTTGAACCACCACCTAC+AGG - chr3.2:77302713-77302732 None:intergenic 45.0%
! AATGGTTATGGTGGCTTGCA+AGG + chr3.2:77303148-77303167 MS.gene06890:CDS 45.0%
! ATGGTTATGGTGGCTTGCAA+GGG + chr3.2:77303149-77303168 MS.gene06890:CDS 45.0%
!! AGAAGGTATTGGACTGAAGG+TGG + chr3.2:77302671-77302690 MS.gene06890:intron 45.0%
!!! GGGTTTTGAGAAGCAGATTG+AGG - chr3.2:77302851-77302870 None:intergenic 45.0%
AATCAAGAGGGTTCTGCTGG+TGG + chr3.2:77303178-77303197 MS.gene06890:CDS 50.0%
! GAATGTTCGGTGGAGGATCA+TGG + chr3.2:77303350-77303369 MS.gene06890:CDS 50.0%
! TGGAATGGAATGTTCGGTGG+AGG + chr3.2:77303343-77303362 MS.gene06890:CDS 50.0%
CAAGAGGGTTCTGCTGGTGG+TGG + chr3.2:77303181-77303200 MS.gene06890:CDS 60.0%
!! GAGGGTTCTGCTGGTGGTGG+TGG + chr3.2:77303184-77303203 MS.gene06890:CDS 65.0%
!! GGTTCTGCTGGTGGTGGTGG+TGG + chr3.2:77303187-77303206 MS.gene06890:CDS 65.0%
!! GTGGTGGTGGTGGTGGTGCA+AGG + chr3.2:77303203-77303222 MS.gene06890:CDS 65.0%
!! TCTGCTGGTGGTGGTGGTGG+TGG + chr3.2:77303190-77303209 MS.gene06890:CDS 65.0%
!! GCTGGTGGTGGTGGTGGTGG+TGG + chr3.2:77303193-77303212 MS.gene06890:CDS 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG + chr3.2:77303196-77303215 MS.gene06890:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 77302500 77303375 77302500 ID=MS.gene06890
chr3.2 mRNA 77302500 77303375 77302500 ID=MS.gene06890.t1;Parent=MS.gene06890
chr3.2 exon 77302500 77302591 77302500 ID=MS.gene06890.t1.exon1;Parent=MS.gene06890.t1
chr3.2 CDS 77302500 77302591 77302500 ID=cds.MS.gene06890.t1;Parent=MS.gene06890.t1
chr3.2 exon 77302673 77302716 77302673 ID=MS.gene06890.t1.exon2;Parent=MS.gene06890.t1
chr3.2 CDS 77302673 77302716 77302673 ID=cds.MS.gene06890.t1;Parent=MS.gene06890.t1
chr3.2 exon 77302834 77303375 77302834 ID=MS.gene06890.t1.exon3;Parent=MS.gene06890.t1
chr3.2 CDS 77302834 77303375 77302834 ID=cds.MS.gene06890.t1;Parent=MS.gene06890.t1
Gene Sequence

>MS.gene06890

ATGGATGGATCAACTTCAAATTCAAAACCAACACCTTCTATGTTAGAGAGAAAAGCAAGGCCACAAAAAGATCAAGCTTTAAACTGTCCAAGGTGCAATTCAACTAACACAAAATTCTGTTACTACAATAACTATAGCCTCTCACAACCAAGGTACTTTTGCAAAACTTGTAGAAGGTATTGGACTGAAGGTGGTTCTTTAAGAAATGTTCCTGTAGGTGGTGGTTCAAGAAAAAACAAAAGATCAACCTCATCATCATCACCTTCAATCTCACAATCACAACCTCCATCATCAATAACAAAAAATATTATCATTAATCCTGATCTTGCTACAGCACAAAATAATTTCCCTCAATCTGCTTCTCAAAACCCTAAGATCCATCAAGGACAAGATCTTAACCTAGCTTATCCACCACAAACAACTGAGGATAATTATAATACCATATCTAAATTCATTGAGCTTCCTTACACAACTGATCAAGACAAAACTACAAGTGCTATGGAGCTTCTGAAAACTGCTTCTTCATCAAGGGGTTTGAATTCTTTTATGCCTATGACTTTGTCTGAGTCTAGTGCAATGTATAATTCAAGTGGTTTTGCTTTGCAAGATTTTAAGCTTGGGGTTAATTTTGGTTTAGAAGGGTTTGAGAATGGTTATGGTGGCTTGCAAGGGATTAATAATCAAGAGGGTTCTGCTGGTGGTGGTGGTGGTGGTGGTGGTGCAAGGATGTTGTTTCCTGTTGTAGATGATCATCAGTTGAATAATAAGCAGCAGCAACAAATTCCAAATAGTGGTGAGTTTGAACATCAGCAAAATAGAACTCAAGGTGATTCTACTGGTTATTGGAATGGAATGTTCGGTGGAGGATCATGGTAG

Protein sequence

>MS.gene06890.t1

MDGSTSNSKPTPSMLERKARPQKDQALNCPRRYWTEGGSLRNVPVAQNNFPQSASQNPKIHQGQDLNLAYPPQTTEDNYNTISKFIELPYTTDQDKTTSAMELLKTASSSRGLNSFMPMTLSESSAMYNSSGFALQDFKLGVNFGLEGFENGYGGLQGINNQEGSAGGGGGGGGARMLFPVVDDHQLNNKQQQQIPNSGEFEHQQNRTQGDSTGYWNGMFGGGSW