AlfalfaGEDB | MS.gene069771

M. sativa cultivar XinJiangDaYe / MS.gene069771

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069771.t1	XP_013452627.1	85.5	220	27	2	1	218	1	217	5.20E-99	370.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069771.t1	Q41015	52.2	207	90	4	1	200	1	205	3.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069771.t1	B7FN38	85.5	220	27	2	1	218	1	217	3.7e-99	370.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049989	MS.gene069771	0.810869	9.30E-51	-1.69E-46
MS.gene050338	MS.gene069771	0.838896	2.19E-57	-1.69E-46
MS.gene05124	MS.gene069771	0.80872	2.70E-50	-1.69E-46
MS.gene051914	MS.gene069771	0.822287	2.57E-53	-1.69E-46
MS.gene052143	MS.gene069771	0.83249	9.18E-56	-1.69E-46
MS.gene052308	MS.gene069771	0.8293	5.56E-55	-1.69E-46
MS.gene052367	MS.gene069771	0.82313	1.63E-53	-1.69E-46
MS.gene052383	MS.gene069771	0.805605	1.23E-49	-1.69E-46
MS.gene053173	MS.gene069771	0.868117	8.29E-66	-1.69E-46
MS.gene053348	MS.gene069771	0.823117	1.65E-53	-1.69E-46
MS.gene053416	MS.gene069771	0.814179	1.76E-51	-1.69E-46
MS.gene053417	MS.gene069771	0.812952	3.27E-51	-1.69E-46
MS.gene054551	MS.gene069771	0.866167	3.47E-65	-1.69E-46
MS.gene054593	MS.gene069771	0.838234	3.25E-57	-1.69E-46
MS.gene054633	MS.gene069771	0.835922	1.27E-56	-1.69E-46
MS.gene055251	MS.gene069771	0.821274	4.41E-53	-1.69E-46
MS.gene055308	MS.gene069771	0.816645	4.97E-52	-1.69E-46
MS.gene055578	MS.gene069771	0.806005	1.01E-49	-1.69E-46
MS.gene056041	MS.gene069771	0.82493	6.18E-54	-1.69E-46
MS.gene056266	MS.gene069771	-0.825869	3.71E-54	-1.69E-46
MS.gene05640	MS.gene069771	0.81966	1.03E-52	-1.69E-46
MS.gene056526	MS.gene069771	0.830567	2.73E-55	-1.69E-46
MS.gene056694	MS.gene069771	0.843666	1.22E-58	-1.69E-46
MS.gene056697	MS.gene069771	0.809204	2.12E-50	-1.69E-46
MS.gene058128	MS.gene069771	0.806733	7.13E-50	-1.69E-46
MS.gene058853	MS.gene069771	0.823189	1.58E-53	-1.69E-46
MS.gene05926	MS.gene069771	0.816051	6.75E-52	-1.69E-46
MS.gene059304	MS.gene069771	0.820375	7.09E-53	-1.69E-46
MS.gene059553	MS.gene069771	0.80718	5.73E-50	-1.69E-46
MS.gene060473	MS.gene069771	0.804019	2.64E-49	-1.69E-46
MS.gene060565	MS.gene069771	0.800548	1.37E-48	-1.69E-46
MS.gene06093	MS.gene069771	0.839173	1.86E-57	-1.69E-46
MS.gene061044	MS.gene069771	0.836651	8.26E-57	-1.69E-46
MS.gene061099	MS.gene069771	0.811022	8.62E-51	-1.69E-46
MS.gene061260	MS.gene069771	0.81379	2.14E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069771.t1	MTR_6g066190	85.973	221	24	3	1	218	1	217	7.99e-132	370
MS.gene069771.t1	MTR_6g009650	74.091	220	45	2	1	218	1	210	3.26e-114	325
MS.gene069771.t1	MTR_6g009563	71.233	219	50	3	1	216	1	209	1.94e-107	308
MS.gene069771.t1	MTR_6g066210	66.667	222	64	2	1	218	1	216	7.97e-103	296
MS.gene069771.t1	MTR_6g059730	55.288	208	85	4	1	202	1	206	1.75e-62	194
MS.gene069771.t1	MTR_6g017225	49.362	235	101	5	1	218	1	234	2.55e-62	199
MS.gene069771.t1	MTR_6g059680	53.810	210	89	4	1	202	1	210	8.59e-52	167
MS.gene069771.t1	MTR_1g035770	42.523	214	109	6	1	202	1	212	8.14e-48	157
MS.gene069771.t1	MTR_6g059810	45.833	216	100	8	1	202	1	213	1.35e-43	145
MS.gene069771.t1	MTR_6g065460	45.192	208	102	6	1	200	1	204	3.32e-39	134
MS.gene069771.t1	MTR_6g059650	40.642	187	100	5	22	201	21	203	2.87e-35	124
MS.gene069771.t1	MTR_6g059760	41.232	211	104	11	1	200	1	202	1.62e-29	109
MS.gene069771.t1	MTR_6g059530	40.000	210	105	9	1	202	1	197	5.39e-28	105
MS.gene069771.t1	MTR_6g065460	40.000	205	93	6	1	200	1	180	5.02e-27	102
MS.gene069771.t1	MTR_6g059410	38.164	207	108	8	5	202	1	196	1.07e-25	99.4
MS.gene069771.t1	MTR_6g045403	37.850	214	115	9	1	203	1	207	4.58e-24	95.9
MS.gene069771.t1	MTR_3g463590	37.500	184	98	7	27	200	27	203	6.21e-23	92.4
MS.gene069771.t1	MTR_6g044780	38.053	226	111	13	1	207	1	216	1.45e-22	91.7
MS.gene069771.t1	MTR_6g045433	36.957	230	113	11	1	207	1	221	1.58e-22	91.7
MS.gene069771.t1	MTR_6g044810	37.445	227	111	13	1	207	1	216	1.96e-22	91.3
MS.gene069771.t1	MTR_6g045097	37.885	227	110	14	1	207	1	216	1.34e-21	89.4
MS.gene069771.t1	MTR_1g057110	31.731	208	116	10	1	202	1	188	9.09e-18	78.2
MS.gene069771.t1	MTR_2g073010	31.731	208	114	11	4	200	8	198	2.53e-11	60.8
MS.gene069771.t1	MTR_6g065570	43.662	71	33	1	4	74	9	72	3.92e-11	58.2
MS.gene069771.t1	MTR_6g078070	30.198	202	129	8	7	200	6	203	9.12e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069771.t1	AT1G73325	29.545	220	125	11	7	208	4	211	3.94e-11	60.8
MS.gene069771.t1	AT1G17860	30.814	172	105	7	6	171	3	166	4.70e-11	60.1

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTAAAACCCATACTAAAT+AGG	0.276635	6.2:-71547910	None:intergenic
CCAAGTATGGTGAATTTAAC+TGG	0.279016	6.2:-71547502	None:intergenic
TATCCTGGTGTAGAATTAAT+TGG	0.295026	6.2:+71547670	MS.gene069771:CDS
CCAGTTAAATTCACCATACT+TGG	0.298543	6.2:+71547502	MS.gene069771:CDS
ACTAGTGATGTTTCCAAGTA+TGG	0.319310	6.2:-71547515	None:intergenic
TGAAACATGGATCTGGATAT+AGG	0.333570	6.2:+71547710	MS.gene069771:CDS
GGACTACAAAAGTTCAATTC+TGG	0.335250	6.2:+71547775	MS.gene069771:CDS
TTACCATGTTTGTCAAATAC+TGG	0.337755	6.2:-71547343	None:intergenic
CTACAAAACAATGCTATAAC+TGG	0.349490	6.2:+71547475	MS.gene069771:CDS
AAAATATAGTATTGGCTACT+AGG	0.372442	6.2:-71547376	None:intergenic
TATTGGCTACTAGGGTTAAG+AGG	0.375270	6.2:-71547367	None:intergenic
GGAAAGGGTGAGGGATAAAA+CGG	0.381013	6.2:-71547269	None:intergenic
GAAACATGGATCTGGATATA+GGG	0.381905	6.2:+71547711	MS.gene069771:CDS
CTGCTAGGGTTATCACTTGC+TGG	0.383052	6.2:-71547400	None:intergenic
GATTGGTGATGAAAGCAAAA+AGG	0.386990	6.2:-71547297	None:intergenic
GAGGGATAAAACGGGTTTCA+TGG	0.388199	6.2:-71547260	None:intergenic
TTTGAAGATTCAGCACAATC+TGG	0.404925	6.2:-71547583	None:intergenic
TCTACACCAGGATAGTTTCC+AGG	0.412867	6.2:-71547661	None:intergenic
TTAATCTTGAAACATGGATC+TGG	0.434400	6.2:+71547703	MS.gene069771:CDS
AGGGTTAGTCCTCCACTGCT+AGG	0.442500	6.2:-71547415	None:intergenic
ATTGTGCTGAATCTTCAAAA+TGG	0.450225	6.2:+71547587	MS.gene069771:CDS
CAACTAAGTTGTGTTGGTAT+TGG	0.455890	6.2:+71547634	MS.gene069771:CDS
CTAAGTTGTGTTGGTATTGG+TGG	0.456720	6.2:+71547637	MS.gene069771:CDS
CTGAATCACCGACTTTGTCT+AGG	0.458530	6.2:-71547435	None:intergenic
AGCGTTGCTTGGTGAGAGAT+TGG	0.473756	6.2:-71547314	None:intergenic
TGTGTTGGTATTGGTGGTCC+TGG	0.481842	6.2:+71547643	MS.gene069771:CDS
GGTGGTCCTGGAAACTATCC+TGG	0.488659	6.2:+71547655	MS.gene069771:CDS
CCACCAATTAATTCTACACC+AGG	0.511123	6.2:-71547673	None:intergenic
AAAGGAAGAAGGAAAGGGTG+AGG	0.524873	6.2:-71547279	None:intergenic
TTGCTGGTAAAATATAGTAT+TGG	0.526997	6.2:-71547384	None:intergenic
CATGGATCTGGATATAGGGT+TGG	0.534082	6.2:+71547715	MS.gene069771:CDS
GGGTTAGTCCTCCACTGCTA+GGG	0.534912	6.2:-71547414	None:intergenic
ACTGGAGATTGTGCTTATGT+TGG	0.540839	6.2:+71547754	MS.gene069771:CDS
GAAAGGGTGAGGGATAAAAC+GGG	0.542290	6.2:-71547268	None:intergenic
ATCACTAGTAACATCGTGAC+AGG	0.548264	6.2:+71547529	MS.gene069771:CDS
ATTAATACTATGTCTAATGA+AGG	0.548963	6.2:+71547868	MS.gene069771:CDS
ATTGGCTACTAGGGTTAAGA+GGG	0.549466	6.2:-71547366	None:intergenic
TGAATCACCGACTTTGTCTA+GGG	0.549511	6.2:-71547434	None:intergenic
AAATATAGTATTGGCTACTA+GGG	0.549975	6.2:-71547375	None:intergenic
AAGGAAGAAGGAAAGGGTGA+GGG	0.562566	6.2:-71547278	None:intergenic
CAAAAGTTCAATTCTGGTGA+AGG	0.564140	6.2:+71547781	MS.gene069771:CDS
CAACCAGTATTTGACAAACA+TGG	0.565333	6.2:+71547340	MS.gene069771:CDS
AATACTCAACTAAGTTGTGT+TGG	0.566729	6.2:+71547628	MS.gene069771:CDS
AAGTTCAATTCTGGTGAAGG+TGG	0.567292	6.2:+71547784	MS.gene069771:CDS
ACTGGTTGAACAGCGTTGCT+TGG	0.579741	6.2:-71547325	None:intergenic
AGTGAACTCGATCTCAAGAT+CGG	0.599342	6.2:-71547554	None:intergenic
CCTGGTGTAGAATTAATTGG+TGG	0.603874	6.2:+71547673	MS.gene069771:CDS
AGTGATAACCCTAGCAGTGG+AGG	0.604496	6.2:+71547406	MS.gene069771:CDS
GCAAGTGATAACCCTAGCAG+TGG	0.634880	6.2:+71547403	MS.gene069771:CDS
GGACTAACCCTAGACAAAGT+CGG	0.647043	6.2:+71547427	MS.gene069771:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTTAATCTTGAAACA+TGG	+	chr6.2:71547697-71547716	MS.gene069771:CDS	15.0%
!!	ATTAATACTATGTCTAATGA+AGG	+	chr6.2:71547868-71547887	MS.gene069771:CDS	20.0%
!	TTGCTGGTAAAATATAGTAT+TGG	-	chr6.2:71547387-71547406	None:intergenic	25.0%
!!!	AAAATATAGTATTGGCTACT+AGG	-	chr6.2:71547379-71547398	None:intergenic	25.0%
!!!	AAATATAGTATTGGCTACTA+GGG	-	chr6.2:71547378-71547397	None:intergenic	25.0%
	AATACTCAACTAAGTTGTGT+TGG	+	chr6.2:71547628-71547647	MS.gene069771:CDS	30.0%
	ATTGTGCTGAATCTTCAAAA+TGG	+	chr6.2:71547587-71547606	MS.gene069771:CDS	30.0%
	CTACAAAACAATGCTATAAC+TGG	+	chr6.2:71547475-71547494	MS.gene069771:CDS	30.0%
	TATCCTGGTGTAGAATTAAT+TGG	+	chr6.2:71547670-71547689	MS.gene069771:CDS	30.0%
	TTAATCTTGAAACATGGATC+TGG	+	chr6.2:71547703-71547722	MS.gene069771:CDS	30.0%
	TTACCATGTTTGTCAAATAC+TGG	-	chr6.2:71547346-71547365	None:intergenic	30.0%
!	ATATAGGGTTGGATTTTGTT+TGG	+	chr6.2:71547726-71547745	MS.gene069771:CDS	30.0%
!!	CAGTTATAGCATTGTTTTGT+AGG	-	chr6.2:71547477-71547496	None:intergenic	30.0%
	AAGCAAAAAGGAAGAAGGAA+AGG	-	chr6.2:71547288-71547307	None:intergenic	35.0%
	ACTAGTGATGTTTCCAAGTA+TGG	-	chr6.2:71547518-71547537	None:intergenic	35.0%
	AGCAAAAAGGAAGAAGGAAA+GGG	-	chr6.2:71547287-71547306	None:intergenic	35.0%
	CAAAAGTTCAATTCTGGTGA+AGG	+	chr6.2:71547781-71547800	MS.gene069771:CDS	35.0%
	CAACCAGTATTTGACAAACA+TGG	+	chr6.2:71547340-71547359	MS.gene069771:CDS	35.0%
	CCAAGTATGGTGAATTTAAC+TGG	-	chr6.2:71547505-71547524	None:intergenic	35.0%
	CCAGTTAAATTCACCATACT+TGG	+	chr6.2:71547502-71547521	MS.gene069771:CDS	35.0%
	GAAACATGGATCTGGATATA+GGG	+	chr6.2:71547711-71547730	MS.gene069771:CDS	35.0%
	GATGAAAGCAAAAAGGAAGA+AGG	-	chr6.2:71547293-71547312	None:intergenic	35.0%
	GGACTACAAAAGTTCAATTC+TGG	+	chr6.2:71547775-71547794	MS.gene069771:CDS	35.0%
	TGAAACATGGATCTGGATAT+AGG	+	chr6.2:71547710-71547729	MS.gene069771:CDS	35.0%
	TTTGAAGATTCAGCACAATC+TGG	-	chr6.2:71547586-71547605	None:intergenic	35.0%
!	CAACTAAGTTGTGTTGGTAT+TGG	+	chr6.2:71547634-71547653	MS.gene069771:CDS	35.0%
!!	GATTGGTGATGAAAGCAAAA+AGG	-	chr6.2:71547300-71547319	None:intergenic	35.0%
!!	GGATTTTGTTTGGATACAAC+TGG	+	chr6.2:71547736-71547755	MS.gene069771:CDS	35.0%
!!	TTGTTTTGTAGGACAGTTAG+TGG	-	chr6.2:71547466-71547485	None:intergenic	35.0%
	AAGTTCAATTCTGGTGAAGG+TGG	+	chr6.2:71547784-71547803	MS.gene069771:CDS	40.0%
	ACTGGAGATTGTGCTTATGT+TGG	+	chr6.2:71547754-71547773	MS.gene069771:CDS	40.0%
	AGTGAACTCGATCTCAAGAT+CGG	-	chr6.2:71547557-71547576	None:intergenic	40.0%
	ATCACTAGTAACATCGTGAC+AGG	+	chr6.2:71547529-71547548	MS.gene069771:CDS	40.0%
	ATTGGCTACTAGGGTTAAGA+GGG	-	chr6.2:71547369-71547388	None:intergenic	40.0%
	CCACCAATTAATTCTACACC+AGG	-	chr6.2:71547676-71547695	None:intergenic	40.0%
	CCTGGTGTAGAATTAATTGG+TGG	+	chr6.2:71547673-71547692	MS.gene069771:CDS	40.0%
!	CTAAGTTGTGTTGGTATTGG+TGG	+	chr6.2:71547637-71547656	MS.gene069771:CDS	40.0%
!	TATTGGCTACTAGGGTTAAG+AGG	-	chr6.2:71547370-71547389	None:intergenic	40.0%
!!	TGAATCACCGACTTTGTCTA+GGG	-	chr6.2:71547437-71547456	None:intergenic	40.0%
	AAAGGAAGAAGGAAAGGGTG+AGG	-	chr6.2:71547282-71547301	None:intergenic	45.0%
	AAGGAAGAAGGAAAGGGTGA+GGG	-	chr6.2:71547281-71547300	None:intergenic	45.0%
	CATGGATCTGGATATAGGGT+TGG	+	chr6.2:71547715-71547734	MS.gene069771:CDS	45.0%
	GAAAGGGTGAGGGATAAAAC+GGG	-	chr6.2:71547271-71547290	None:intergenic	45.0%
	GGAAAGGGTGAGGGATAAAA+CGG	-	chr6.2:71547272-71547291	None:intergenic	45.0%
	GGACTAACCCTAGACAAAGT+CGG	+	chr6.2:71547427-71547446	MS.gene069771:CDS	45.0%
	TCTACACCAGGATAGTTTCC+AGG	-	chr6.2:71547664-71547683	None:intergenic	45.0%
!!	CTGAATCACCGACTTTGTCT+AGG	-	chr6.2:71547438-71547457	None:intergenic	45.0%
	ACTGGTTGAACAGCGTTGCT+TGG	-	chr6.2:71547328-71547347	None:intergenic	50.0%
	AGTGATAACCCTAGCAGTGG+AGG	+	chr6.2:71547406-71547425	MS.gene069771:CDS	50.0%
	CTGCTAGGGTTATCACTTGC+TGG	-	chr6.2:71547403-71547422	None:intergenic	50.0%
	GCAAGTGATAACCCTAGCAG+TGG	+	chr6.2:71547403-71547422	MS.gene069771:CDS	50.0%
!	AGCGTTGCTTGGTGAGAGAT+TGG	-	chr6.2:71547317-71547336	None:intergenic	50.0%
!!	TGTGTTGGTATTGGTGGTCC+TGG	+	chr6.2:71547643-71547662	MS.gene069771:CDS	50.0%
	GGTGGTCCTGGAAACTATCC+TGG	+	chr6.2:71547655-71547674	MS.gene069771:CDS	55.0%
!!	AGGGTTAGTCCTCCACTGCT+AGG	-	chr6.2:71547418-71547437	None:intergenic	55.0%
!!	GGGTTAGTCCTCCACTGCTA+GGG	-	chr6.2:71547417-71547436	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	71547262	71547918	71547262	ID=MS.gene069771
chr6.2	mRNA	71547262	71547918	71547262	ID=MS.gene069771.t1;Parent=MS.gene069771
chr6.2	exon	71547262	71547918	71547262	ID=MS.gene069771.t1.exon1;Parent=MS.gene069771.t1
chr6.2	CDS	71547262	71547918	71547262	ID=cds.MS.gene069771.t1;Parent=MS.gene069771.t1

Gene Sequence

>MS.gene069771

ATGAAACCCGTTTTATCCCTCACCCTTTCCTTCTTCCTTTTTGCTTTCATCACCAATCTCTCACCAAGCAACGCTGTTCAACCAGTATTTGACAAACATGGTAACCCTCTTAACCCTAGTAGCCAATACTATATTTTACCAGCAAGTGATAACCCTAGCAGTGGAGGACTAACCCTAGACAAAGTCGGTGATTCAGAGTGTCCACTAACTGTCCTACAAAACAATGCTATAACTGGTTTACCAGTTAAATTCACCATACTTGGAAACATCACTAGTAACATCGTGACAGGTACCGATCTTGAGATCGAGTTCACTAAGAAGCCAGATTGTGCTGAATCTTCAAAATGGTTGATGTTTGTTGATCATAATACTCAACTAAGTTGTGTTGGTATTGGTGGTCCTGGAAACTATCCTGGTGTAGAATTAATTGGTGGCAAATTTTTAATCTTGAAACATGGATCTGGATATAGGGTTGGATTTTGTTTGGATACAACTGGAGATTGTGCTTATGTTGGACTACAAAAGTTCAATTCTGGTGAAGGTGGATCACGTTTGATTTTGACTGTAACTGATGCTTATTCTGTTGTATTTGTTGATGCTGCTTCTATTAATACTATGTCTAATGAAGGTAATGCAAATAGTGCATTGCCTATTTAG

Protein sequence

>MS.gene069771.t1

MKPVLSLTLSFFLFAFITNLSPSNAVQPVFDKHGNPLNPSSQYYILPASDNPSSGGLTLDKVGDSECPLTVLQNNAITGLPVKFTILGNITSNIVTGTDLEIEFTKKPDCAESSKWLMFVDHNTQLSCVGIGGPGNYPGVELIGGKFLILKHGSGYRVGFCLDTTGDCAYVGLQKFNSGEGGSRLILTVTDAYSVVFVDAASINTMSNEGNANSALPI