Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072602.t1 | XP_003626135.2 | 100 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 2.40E-21 | 110.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072602.t1 | Q9ZNS6 | 81.5 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 7.3e-19 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072602.t1 | Q2HRR6 | 100.0 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 1.7e-21 | 110.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene072602 | -0.827929 | 1.19E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene072602 | -0.80288 | 4.55E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene072602 | 0.807447 | 5.03E-50 | -1.69E-46 |
| MS.gene050800 | MS.gene072602 | 0.934797 | 2.31E-96 | -1.69E-46 |
| MS.gene051113 | MS.gene072602 | 0.835861 | 1.31E-56 | -1.69E-46 |
| MS.gene051694 | MS.gene072602 | -0.843365 | 1.47E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene072602 | -0.806697 | 7.25E-50 | -1.69E-46 |
| MS.gene05234 | MS.gene072602 | 0.820008 | 8.60E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene072602 | -0.817708 | 2.87E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene072602 | -0.803638 | 3.17E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene072602 | -0.819872 | 9.24E-53 | -1.69E-46 |
| MS.gene052825 | MS.gene072602 | -0.817594 | 3.04E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene072602 | -0.805527 | 1.28E-49 | -1.69E-46 |
| MS.gene053286 | MS.gene072602 | -0.800209 | 1.60E-48 | -1.69E-46 |
| MS.gene053421 | MS.gene072602 | 0.813068 | 3.09E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene072602 | -0.819978 | 8.74E-53 | -1.69E-46 |
| MS.gene054706 | MS.gene072602 | 0.805975 | 1.03E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene072602 | -0.830433 | 2.94E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene072602 | -0.803367 | 3.61E-49 | -1.69E-46 |
| MS.gene056942 | MS.gene072602 | -0.815834 | 7.54E-52 | -1.69E-46 |
| MS.gene05726 | MS.gene072602 | 0.803671 | 3.12E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene072602 | -0.800117 | 1.68E-48 | -1.69E-46 |
| MS.gene058157 | MS.gene072602 | -0.844302 | 8.22E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene072602 | -0.800421 | 1.45E-48 | -1.69E-46 |
| MS.gene059718 | MS.gene072602 | -0.822596 | 2.18E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene072602 | -0.806506 | 7.96E-50 | -1.69E-46 |
| MS.gene060602 | MS.gene072602 | 0.802492 | 5.47E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072602.t1 | MTR_7g111450 | 100.000 | 54 | 0 | 0 | 1 | 54 | 1 | 54 | 8.33e-33 | 106 |
| MS.gene072602.t1 | MTR_7g111380 | 87.037 | 54 | 7 | 0 | 1 | 54 | 1 | 54 | 2.10e-29 | 98.2 |
| MS.gene072602.t1 | MTR_7g111350 | 81.481 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 2.25e-26 | 90.5 |
| MS.gene072602.t1 | MTR_6g033495 | 61.538 | 52 | 20 | 0 | 1 | 52 | 1 | 52 | 1.67e-19 | 73.2 |
| MS.gene072602.t1 | MTR_4g130675 | 62.500 | 48 | 17 | 1 | 6 | 52 | 8 | 55 | 8.08e-14 | 60.5 |
| MS.gene072602.t1 | MTR_4g130660 | 56.522 | 46 | 19 | 1 | 8 | 52 | 10 | 55 | 7.41e-12 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072602.t1 | AT3G05890 | 81.481 | 54 | 10 | 0 | 1 | 54 | 1 | 54 | 1.83e-26 | 90.9 |
| MS.gene072602.t1 | AT3G05880 | 75.926 | 54 | 13 | 0 | 1 | 54 | 1 | 54 | 3.56e-25 | 87.4 |
| MS.gene072602.t1 | AT2G38905 | 62.264 | 53 | 20 | 0 | 1 | 53 | 1 | 53 | 2.73e-20 | 75.1 |
| MS.gene072602.t1 | AT1G57550 | 50.980 | 51 | 25 | 0 | 4 | 54 | 2 | 52 | 1.47e-15 | 63.2 |
| MS.gene072602.t1 | AT4G28088 | 57.692 | 52 | 21 | 1 | 2 | 52 | 4 | 55 | 9.79e-15 | 61.6 |
| MS.gene072602.t1 | AT2G24040 | 64.583 | 48 | 16 | 1 | 6 | 52 | 8 | 55 | 1.34e-14 | 61.2 |
| MS.gene072602.t1 | AT4G30650 | 65.217 | 46 | 15 | 1 | 8 | 52 | 10 | 55 | 4.59e-14 | 60.1 |
| MS.gene072602.t1 | AT4G30660 | 67.391 | 46 | 14 | 1 | 8 | 52 | 10 | 55 | 2.93e-13 | 58.2 |
| MS.gene072602.t1 | AT4G30660 | 67.391 | 46 | 14 | 1 | 8 | 52 | 10 | 55 | 2.93e-13 | 58.2 |
Find 0 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAATTAATCAATTGTT+TGG | - | 50598:40891-40910 | MS.gene072602:intron | 10.0% |
| !! | CAAAAAAAAAAGAAGAAGAA+AGG | + | 50598:40853-40872 | None:intergenic | 20.0% |
| !! | TTAATTAATCAATTGTTTGG+TGG | - | 50598:40894-40913 | MS.gene072602:intron | 20.0% |
| !! | TTGGTGGTAATGTATTATAT+AGG | - | 50598:40910-40929 | MS.gene072602:intron | 25.0% |
| ! | GTGGTAATGTATTATATAGG+TGG | - | 50598:40913-40932 | MS.gene072602:intron | 30.0% |
| !!! | GGAAGAGAATTTTAGTTACA+TGG | + | 50598:40832-40851 | None:intergenic | 30.0% |
| AGATAGCATAGAGAATTCCA+GGG | + | 50598:40979-40998 | None:intergenic | 35.0% | |
| GTATTATATAGGTGGAGTTC+TGG | - | 50598:40921-40940 | MS.gene072602:intron | 35.0% | |
| TAGATAGCATAGAGAATTCC+AGG | + | 50598:40980-40999 | None:intergenic | 35.0% | |
| !!! | TGTTTGGTGCTAACCCTTTT+TGG | - | 50598:40947-40966 | MS.gene072602:CDS | 40.0% |
| AACTTGAGGAAGACACCAAG+AGG | + | 50598:40803-40822 | None:intergenic | 45.0% | |
| TCCAGGGAGATAGCCAAAAA+GGG | + | 50598:40963-40982 | None:intergenic | 45.0% | |
| TTACATGGCAGCCAAACTTG+AGG | + | 50598:40817-40836 | None:intergenic | 45.0% | |
| TTCCAGGGAGATAGCCAAAA+AGG | + | 50598:40964-40983 | None:intergenic | 45.0% | |
| !! | CTTGGTGTCTTCCTCAAGTT+TGG | - | 50598:40803-40822 | MS.gene072602:CDS | 45.0% |
| TGAGGAAGACACCAAGAGGA+GGG | + | 50598:40799-40818 | None:intergenic | 50.0% | |
| TTGAGGAAGACACCAAGAGG+AGG | + | 50598:40800-40819 | None:intergenic | 50.0% | |
| !!! | ACCCTTTTTGGCTATCTCCC+TGG | - | 50598:40959-40978 | MS.gene072602:CDS | 50.0% |
| ACCAAGAGGAGGGAGGATGA+TGG | + | 50598:40789-40808 | None:intergenic | 55.0% | |
| !! | GGTGGAGTTCTGGCTCTGTT+TGG | - | 50598:40931-40950 | MS.gene072602:intron | 55.0% |
| GCCATCATCCTCCCTCCTCT+TGG | - | 50598:40785-40804 | MS.gene072602:CDS | 60.0% | |
| GGAAGACACCAAGAGGAGGG+AGG | + | 50598:40796-40815 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50598 | gene | 40752 | 41015 | 40752 | ID=MS.gene072602 |
| 50598 | mRNA | 40752 | 41015 | 40752 | ID=MS.gene072602.t1;Parent=MS.gene072602 |
| 50598 | exon | 40935 | 41015 | 40935 | ID=MS.gene072602.t1.exon1;Parent=MS.gene072602.t1 |
| 50598 | CDS | 40935 | 41015 | 40935 | ID=cds.MS.gene072602.t1;Parent=MS.gene072602.t1 |
| 50598 | exon | 40752 | 40835 | 40752 | ID=MS.gene072602.t1.exon2;Parent=MS.gene072602.t1 |
| 50598 | CDS | 40752 | 40835 | 40752 | ID=cds.MS.gene072602.t1;Parent=MS.gene072602.t1 |
| Gene Sequence |
| Protein sequence |
