Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072900.t1 | XP_013461885.1 | 97.9 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 5.90E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072900.t1 | Q9SN73 | 50.6 | 81 | 40 | 0 | 60 | 140 | 56 | 136 | 2.0e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072900.t1 | A0A072VCQ1 | 97.9 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 4.3e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene072900 | 0.818665 | 1.74E-52 | -1.69E-46 |
| MS.gene050404 | MS.gene072900 | 0.877117 | 8.13E-69 | -1.69E-46 |
| MS.gene050406 | MS.gene072900 | 0.871374 | 7.18E-67 | -1.69E-46 |
| MS.gene050464 | MS.gene072900 | 0.805115 | 1.56E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene072900 | 0.834377 | 3.11E-56 | -1.69E-46 |
| MS.gene050812 | MS.gene072900 | 0.805487 | 1.30E-49 | -1.69E-46 |
| MS.gene051171 | MS.gene072900 | 0.876286 | 1.58E-68 | -1.69E-46 |
| MS.gene051240 | MS.gene072900 | 0.842687 | 2.22E-58 | -1.69E-46 |
| MS.gene051242 | MS.gene072900 | 0.880125 | 7.10E-70 | -1.69E-46 |
| MS.gene051244 | MS.gene072900 | 0.849593 | 2.93E-60 | -1.69E-46 |
| MS.gene051471 | MS.gene072900 | 0.810829 | 9.49E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene072900 | 0.833316 | 5.72E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene072900 | 0.801467 | 8.88E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene072900 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene053330 | MS.gene072900 | 0.877741 | 4.93E-69 | -1.69E-46 |
| MS.gene053907 | MS.gene072900 | 0.86703 | 1.85E-65 | -1.69E-46 |
| MS.gene053971 | MS.gene072900 | 0.914708 | 1.40E-84 | -1.69E-46 |
| MS.gene054983 | MS.gene072900 | 0.801635 | 8.21E-49 | -1.69E-46 |
| MS.gene055370 | MS.gene072900 | 0.856496 | 3.09E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene072900 | 0.813116 | 3.01E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene072900 | 0.815456 | 9.16E-52 | -1.69E-46 |
| MS.gene055980 | MS.gene072900 | 0.811804 | 5.83E-51 | -1.69E-46 |
| MS.gene056305 | MS.gene072900 | 0.814429 | 1.55E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene072900 | 0.85616 | 3.88E-62 | -1.69E-46 |
| MS.gene056612 | MS.gene072900 | 0.842082 | 3.21E-58 | -1.69E-46 |
| MS.gene056633 | MS.gene072900 | 0.917662 | 4.04E-86 | -1.69E-46 |
| MS.gene056664 | MS.gene072900 | 0.901295 | 3.09E-78 | -1.69E-46 |
| MS.gene05702 | MS.gene072900 | 0.827857 | 1.24E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene072900 | 0.812733 | 3.66E-51 | -1.69E-46 |
| MS.gene058233 | MS.gene072900 | 0.888871 | 4.02E-73 | -1.69E-46 |
| MS.gene058276 | MS.gene072900 | 0.865386 | 6.13E-65 | -1.69E-46 |
| MS.gene058743 | MS.gene072900 | 0.805627 | 1.22E-49 | -1.69E-46 |
| MS.gene058746 | MS.gene072900 | 0.932363 | 9.53E-95 | -1.69E-46 |
| MS.gene059894 | MS.gene072900 | 0.85115 | 1.07E-60 | -1.69E-46 |
| MS.gene060407 | MS.gene072900 | 0.852455 | 4.57E-61 | -1.69E-46 |
| MS.gene061049 | MS.gene072900 | 0.831743 | 1.40E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072900.t1 | MTR_3g107397 | 97.857 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 4.37e-99 | 280 |
| MS.gene072900.t1 | MTR_4g114180 | 52.885 | 104 | 48 | 1 | 37 | 139 | 29 | 132 | 5.56e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072900.t1 | AT3G47650 | 69.136 | 81 | 25 | 0 | 60 | 140 | 56 | 136 | 3.31e-37 | 124 |
Find 35 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATTTCAGGGTCAATTA+TGG | 0.200301 | 3.2:+87166001 | MS.gene072900:CDS |
| GTGCCTCCTGTTGGCTTTGC+GGG | 0.255239 | 3.2:+87166520 | MS.gene072900:CDS |
| TCAGCACAGATGACACTATT+TGG | 0.263290 | 3.2:-87166192 | None:intergenic |
| ATACGAACCGAAAACATTTC+AGG | 0.285412 | 3.2:+87165990 | MS.gene072900:CDS |
| TGCAATGGAGCTGGATTTAT+CGG | 0.322631 | 3.2:+87166776 | MS.gene072900:CDS |
| GTTCAAACTTCTGAATGTTC+AGG | 0.329988 | 3.2:+87165944 | MS.gene072900:CDS |
| AACATTTCAGGGTCAATTAT+GGG | 0.350289 | 3.2:+87166002 | MS.gene072900:CDS |
| ATTTATCTGTGGGAGGGCCT+TGG | 0.381621 | 3.2:-87166027 | None:intergenic |
| TGCCTAACACCTTCATGTCT+TGG | 0.395804 | 3.2:+87165905 | MS.gene072900:CDS |
| GGTGCCTCCTGTTGGCTTTG+CGG | 0.447694 | 3.2:+87166519 | MS.gene072900:CDS |
| TTCGATGGACTGTTTAAAGC+TGG | 0.460757 | 3.2:+87166498 | MS.gene072900:CDS |
| TACGAACCGAAAACATTTCA+GGG | 0.468265 | 3.2:+87165991 | MS.gene072900:CDS |
| TAGGTACCCGCAAAGCCAAC+AGG | 0.473779 | 3.2:-87166526 | None:intergenic |
| TGCCAAGACATGAAGGTGTT+AGG | 0.493946 | 3.2:-87165907 | None:intergenic |
| TTAAAGCTGGTGCCTCCTGT+TGG | 0.514045 | 3.2:+87166511 | MS.gene072900:CDS |
| TGTGGGAATTGCAATGGAGC+TGG | 0.521634 | 3.2:+87166767 | MS.gene072900:CDS |
| AGGGTCAATTATGGGTTCCA+AGG | 0.522125 | 3.2:+87166010 | MS.gene072900:CDS |
| GGCAGGAAGGAGATGTTATG+TGG | 0.530714 | 3.2:+87166749 | MS.gene072900:CDS |
| TGTGCTGATTGTGATGGAAA+TGG | 0.538508 | 3.2:+87166207 | MS.gene072900:CDS |
| GCAGGAAGGAGATGTTATGT+GGG | 0.542628 | 3.2:+87166750 | MS.gene072900:CDS |
| TGCTCTCAATGCAAAGGTAG+CGG | 0.551522 | 3.2:+87166456 | MS.gene072900:CDS |
| CTTGAGCACATATGACCAGT+AGG | 0.554604 | 3.2:+87166805 | MS.gene072900:CDS |
| GACAAGGCAATATTTATCTG+TGG | 0.574721 | 3.2:-87166038 | None:intergenic |
| GGCAATATTTATCTGTGGGA+GGG | 0.576292 | 3.2:-87166033 | None:intergenic |
| ACAAGGCAATATTTATCTGT+GGG | 0.583163 | 3.2:-87166037 | None:intergenic |
| AGGCAAAGAGCAGATACCAT+TGG | 0.591049 | 3.2:-87165887 | None:intergenic |
| ATGTTATGTGGGAATTGCAA+TGG | 0.609174 | 3.2:+87166761 | MS.gene072900:CDS |
| AATGGAGCTGGATTTATCGG+TGG | 0.609254 | 3.2:+87166779 | MS.gene072900:CDS |
| AATGTACCTTTGCAGTGACA+AGG | 0.612316 | 3.2:-87166054 | None:intergenic |
| TTGAGCACATATGACCAGTA+GGG | 0.613108 | 3.2:+87166806 | MS.gene072900:CDS |
| AGGCAATATTTATCTGTGGG+AGG | 0.615579 | 3.2:-87166034 | None:intergenic |
| ATTGTCTGCTCTCAATGCAA+AGG | 0.622596 | 3.2:+87166450 | MS.gene072900:CDS |
| GTCATCTGTGCTGATTGTGA+TGG | 0.623087 | 3.2:+87166201 | MS.gene072900:CDS |
| ATATTGCCTTGTCACTGCAA+AGG | 0.634181 | 3.2:+87166048 | MS.gene072900:CDS |
| GTACCCGCAAAGCCAACAGG+AGG | 0.718423 | 3.2:-87166523 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTGCATTTAACAATT+AGG | - | chr3.2:87166608-87166627 | None:intergenic | 15.0% |
| !!! | ATAGAATGATTTTACTAATT+AGG | - | chr3.2:87166548-87166567 | None:intergenic | 15.0% |
| !!! | TTATATTTTGTGTAATTAAC+AGG | + | chr3.2:87166423-87166442 | MS.gene072900:intron | 15.0% |
| !! | CGATGAACAATAATAATATA+AGG | - | chr3.2:87166715-87166734 | None:intergenic | 20.0% |
| !!! | AGATGACATATGATTTTATA+AGG | + | chr3.2:87166232-87166251 | MS.gene072900:intron | 20.0% |
| ! | CAAGAGAAAATATACATCTA+CGG | + | chr3.2:87166387-87166406 | MS.gene072900:intron | 25.0% |
| ! | TAATAATATAAGGCACATTG+TGG | - | chr3.2:87166705-87166724 | None:intergenic | 25.0% |
| !!! | TATTTGGTTTTGTGTTTTGT+TGG | - | chr3.2:87166179-87166198 | None:intergenic | 25.0% |
| AAACATTTCAGGGTCAATTA+TGG | + | chr3.2:87166001-87166020 | MS.gene072900:CDS | 30.0% | |
| AACATTTCAGGGTCAATTAT+GGG | + | chr3.2:87166002-87166021 | MS.gene072900:CDS | 30.0% | |
| AACGATTTACATGCTCATAA+CGG | - | chr3.2:87166313-87166332 | None:intergenic | 30.0% | |
| AATGTATTTGTGGTTGTTGA+AGG | + | chr3.2:87166358-87166377 | MS.gene072900:intron | 30.0% | |
| ACAAGGCAATATTTATCTGT+GGG | - | chr3.2:87166040-87166059 | None:intergenic | 30.0% | |
| TGCTTCTTGTAATGTATTTG+TGG | + | chr3.2:87166348-87166367 | MS.gene072900:intron | 30.0% | |
| ! | AACTCTGTTGATATTTTCGA+TGG | + | chr3.2:87166483-87166502 | MS.gene072900:CDS | 30.0% |
| ! | AATTGACCCTGAAATGTTTT+CGG | - | chr3.2:87166000-87166019 | None:intergenic | 30.0% |
| AAGGCTTCATTGTATTCTAG+AGG | - | chr3.2:87166643-87166662 | None:intergenic | 35.0% | |
| ATACGAACCGAAAACATTTC+AGG | + | chr3.2:87165990-87166009 | MS.gene072900:CDS | 35.0% | |
| ATGTTATGTGGGAATTGCAA+TGG | + | chr3.2:87166761-87166780 | MS.gene072900:CDS | 35.0% | |
| GACAAGGCAATATTTATCTG+TGG | - | chr3.2:87166041-87166060 | None:intergenic | 35.0% | |
| GTTCAAACTTCTGAATGTTC+AGG | + | chr3.2:87165944-87165963 | MS.gene072900:CDS | 35.0% | |
| TACGAACCGAAAACATTTCA+GGG | + | chr3.2:87165991-87166010 | MS.gene072900:CDS | 35.0% | |
| ! | AATGAAGCCTTATGATCAGT+AGG | + | chr3.2:87166652-87166671 | MS.gene072900:intron | 35.0% |
| ! | ATGAAGCCTTATGATCAGTA+GGG | + | chr3.2:87166653-87166672 | MS.gene072900:intron | 35.0% |
| ! | CACTGCAAAGGTACATTTAT+GGG | + | chr3.2:87166060-87166079 | MS.gene072900:intron | 35.0% |
| ! | TCACTGCAAAGGTACATTTA+TGG | + | chr3.2:87166059-87166078 | MS.gene072900:intron | 35.0% |
| !! | ATTTTAGTGCCAAGACATGA+AGG | - | chr3.2:87165917-87165936 | None:intergenic | 35.0% |
| !!! | TTCATCGTTTTTTACTCCAG+AGG | + | chr3.2:87166728-87166747 | MS.gene072900:intron | 35.0% |
| AATGTACCTTTGCAGTGACA+AGG | - | chr3.2:87166057-87166076 | None:intergenic | 40.0% | |
| AGGCAATATTTATCTGTGGG+AGG | - | chr3.2:87166037-87166056 | None:intergenic | 40.0% | |
| ATATTGCCTTGTCACTGCAA+AGG | + | chr3.2:87166048-87166067 | MS.gene072900:CDS | 40.0% | |
| ATTGTCTGCTCTCAATGCAA+AGG | + | chr3.2:87166450-87166469 | MS.gene072900:CDS | 40.0% | |
| GGCAATATTTATCTGTGGGA+GGG | - | chr3.2:87166036-87166055 | None:intergenic | 40.0% | |
| TCAGCACAGATGACACTATT+TGG | - | chr3.2:87166195-87166214 | None:intergenic | 40.0% | |
| TGCAATGGAGCTGGATTTAT+CGG | + | chr3.2:87166776-87166795 | MS.gene072900:CDS | 40.0% | |
| !! | TGTGCTGATTGTGATGGAAA+TGG | + | chr3.2:87166207-87166226 | MS.gene072900:CDS | 40.0% |
| !! | TTCGATGGACTGTTTAAAGC+TGG | + | chr3.2:87166498-87166517 | MS.gene072900:CDS | 40.0% |
| AATGGAGCTGGATTTATCGG+TGG | + | chr3.2:87166779-87166798 | MS.gene072900:CDS | 45.0% | |
| AGGGTCAATTATGGGTTCCA+AGG | + | chr3.2:87166010-87166029 | MS.gene072900:CDS | 45.0% | |
| GCAGGAAGGAGATGTTATGT+GGG | + | chr3.2:87166750-87166769 | MS.gene072900:CDS | 45.0% | |
| GTTCCTCCCTACTGATCATA+AGG | - | chr3.2:87166662-87166681 | None:intergenic | 45.0% | |
| TGCCTAACACCTTCATGTCT+TGG | + | chr3.2:87165905-87165924 | MS.gene072900:CDS | 45.0% | |
| TGCTCTCAATGCAAAGGTAG+CGG | + | chr3.2:87166456-87166475 | MS.gene072900:CDS | 45.0% | |
| ! | AAGCCTTATGATCAGTAGGG+AGG | + | chr3.2:87166656-87166675 | MS.gene072900:intron | 45.0% |
| ! | TTTTTACTCCAGAGGCAGGA+AGG | + | chr3.2:87166736-87166755 | MS.gene072900:intron | 45.0% |
| !! | GTCATCTGTGCTGATTGTGA+TGG | + | chr3.2:87166201-87166220 | MS.gene072900:CDS | 45.0% |
| !! | TGCCAAGACATGAAGGTGTT+AGG | - | chr3.2:87165910-87165929 | None:intergenic | 45.0% |
| !!! | TCGTTTTTTACTCCAGAGGC+AGG | + | chr3.2:87166732-87166751 | MS.gene072900:intron | 45.0% |
| !! | TAATTATTTATTTATTATGT+AGG | + | chr3.2:87166140-87166159 | MS.gene072900:intron | 5.0% |
| ATTTATCTGTGGGAGGGCCT+TGG | - | chr3.2:87166030-87166049 | None:intergenic | 50.0% | |
| GGCAGGAAGGAGATGTTATG+TGG | + | chr3.2:87166749-87166768 | MS.gene072900:CDS | 50.0% | |
| TAACATCTCCTTCCTGCCTC+TGG | - | chr3.2:87166747-87166766 | None:intergenic | 50.0% | |
| TGTGGGAATTGCAATGGAGC+TGG | + | chr3.2:87166767-87166786 | MS.gene072900:CDS | 50.0% | |
| TTAAAGCTGGTGCCTCCTGT+TGG | + | chr3.2:87166511-87166530 | MS.gene072900:CDS | 50.0% | |
| TAGGTACCCGCAAAGCCAAC+AGG | - | chr3.2:87166529-87166548 | None:intergenic | 55.0% | |
| GTACCCGCAAAGCCAACAGG+AGG | - | chr3.2:87166526-87166545 | None:intergenic | 60.0% | |
| !! | GGTGCCTCCTGTTGGCTTTG+CGG | + | chr3.2:87166519-87166538 | MS.gene072900:CDS | 60.0% |
| !! | GTGCCTCCTGTTGGCTTTGC+GGG | + | chr3.2:87166520-87166539 | MS.gene072900:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 87165890 | 87166826 | 87165890 | ID=MS.gene072900 |
| chr3.2 | mRNA | 87165890 | 87166826 | 87165890 | ID=MS.gene072900.t1;Parent=MS.gene072900 |
| chr3.2 | exon | 87165890 | 87166069 | 87165890 | ID=MS.gene072900.t1.exon1;Parent=MS.gene072900.t1 |
| chr3.2 | CDS | 87165890 | 87166069 | 87165890 | ID=cds.MS.gene072900.t1;Parent=MS.gene072900.t1 |
| chr3.2 | exon | 87166162 | 87166228 | 87166162 | ID=MS.gene072900.t1.exon2;Parent=MS.gene072900.t1 |
| chr3.2 | CDS | 87166162 | 87166228 | 87166162 | ID=cds.MS.gene072900.t1;Parent=MS.gene072900.t1 |
| chr3.2 | exon | 87166445 | 87166541 | 87166445 | ID=MS.gene072900.t1.exon3;Parent=MS.gene072900.t1 |
| chr3.2 | CDS | 87166445 | 87166541 | 87166445 | ID=cds.MS.gene072900.t1;Parent=MS.gene072900.t1 |
| chr3.2 | exon | 87166748 | 87166826 | 87166748 | ID=MS.gene072900.t1.exon4;Parent=MS.gene072900.t1 |
| chr3.2 | CDS | 87166748 | 87166826 | 87166748 | ID=cds.MS.gene072900.t1;Parent=MS.gene072900.t1 |
| Gene Sequence |
| Protein sequence |
