Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073244.t1 | KEH29628.1 | 98.2 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 5.60E-49 | 203.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073244.t1 | A0A072UKK4 | 98.2 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 4.0e-49 | 203.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene073244 | 0.805473 | 1.31E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene073244 | 0.849977 | 2.29E-60 | -1.69E-46 |
| MS.gene049499 | MS.gene073244 | 0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene050414 | MS.gene073244 | 0.83378 | 4.38E-56 | -1.69E-46 |
| MS.gene050945 | MS.gene073244 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene051637 | MS.gene073244 | 0.810663 | 1.03E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene073244 | 0.850927 | 1.24E-60 | -1.69E-46 |
| MS.gene052016 | MS.gene073244 | 0.809737 | 1.63E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene073244 | 0.858801 | 6.42E-63 | -1.69E-46 |
| MS.gene052793 | MS.gene073244 | 0.803489 | 3.40E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene073244 | 0.80486 | 1.76E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene073244 | 0.836767 | 7.72E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene073244 | 0.84087 | 6.70E-58 | -1.69E-46 |
| MS.gene054957 | MS.gene073244 | 0.809901 | 1.50E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene073244 | 0.807567 | 4.74E-50 | -1.69E-46 |
| MS.gene05624 | MS.gene073244 | 0.825735 | 3.99E-54 | -1.69E-46 |
| MS.gene056677 | MS.gene073244 | 0.815514 | 8.89E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene073244 | 0.80628 | 8.88E-50 | -1.69E-46 |
| MS.gene057155 | MS.gene073244 | 0.843028 | 1.80E-58 | -1.69E-46 |
| MS.gene057622 | MS.gene073244 | 0.802782 | 4.77E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene073244 | 0.802487 | 5.48E-49 | -1.69E-46 |
| MS.gene058279 | MS.gene073244 | 0.807226 | 5.61E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene073244 | 0.807853 | 4.13E-50 | -1.69E-46 |
| MS.gene05878 | MS.gene073244 | 0.807773 | 4.29E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene073244 | 0.845024 | 5.26E-59 | -1.69E-46 |
| MS.gene05985 | MS.gene073244 | -0.828621 | 8.12E-55 | -1.69E-46 |
| MS.gene06018 | MS.gene073244 | 0.803879 | 2.82E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene073244 | 0.826365 | 2.82E-54 | -1.69E-46 |
| MS.gene060412 | MS.gene073244 | 0.816253 | 6.08E-52 | -1.69E-46 |
| MS.gene060413 | MS.gene073244 | 0.805613 | 1.23E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene073244 | 0.815202 | 1.04E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene073244 | 0.806537 | 7.84E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene073244 | 0.811616 | 6.40E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073244.t1 | MTR_4g046147 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 3.09e-73 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAAAAGACCCAAGATAAAA+AGG | 0.220778 | 4.3:+55116745 | MS.gene073244:CDS |
| CCTCAAAGGATCTTCCTAAT+AGG | 0.333048 | 4.3:-55116876 | None:intergenic |
| CTAGCTCTCCTGTTCCTATT+AGG | 0.342733 | 4.3:+55116862 | MS.gene073244:CDS |
| AGACAAGGTTATGAGCTTCT+TGG | 0.395080 | 4.3:+55116603 | MS.gene073244:CDS |
| CCTATTAGGAAGATCCTTTG+AGG | 0.449804 | 4.3:+55116876 | MS.gene073244:CDS |
| AGGATCTTCCTAATAGGAAC+AGG | 0.452269 | 4.3:-55116870 | None:intergenic |
| TATGAGCTTCTTGGTTTACA+AGG | 0.464864 | 4.3:+55116612 | MS.gene073244:CDS |
| GTTGCGAACAAGGGTGTTCA+TGG | 0.470725 | 4.3:-55116559 | None:intergenic |
| ACACCACTCTCACCTTCAGC+AGG | 0.551528 | 4.3:+55116580 | MS.gene073244:CDS |
| TGAGAGTGGTGTTGCGAACA+AGG | 0.556014 | 4.3:-55116569 | None:intergenic |
| TCTCCTGCTGAAGGTGAGAG+TGG | 0.568581 | 4.3:-55116583 | None:intergenic |
| ATAACCTTGTCTCCTGCTGA+AGG | 0.573023 | 4.3:-55116592 | None:intergenic |
| ATGAGCTTCTTGGTTTACAA+GGG | 0.601511 | 4.3:+55116613 | MS.gene073244:CDS |
| CTCACCTTCAGCAGGAGACA+AGG | 0.603930 | 4.3:+55116588 | MS.gene073244:CDS |
| GAAAGAGAAAACACTCAAGT+CGG | 0.616749 | 4.3:+55116635 | MS.gene073244:CDS |
| GACGCAGAAGAGCCAAACAA+AGG | 0.632309 | 4.3:+55116667 | MS.gene073244:CDS |
| GAGAGTGGTGTTGCGAACAA+GGG | 0.709541 | 4.3:-55116568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAAGACCCAAGATAAAAA+GGG | + | chr4.3:55116746-55116765 | MS.gene073244:CDS | 25.0% |
| CTAAAAGACCCAAGATAAAA+AGG | + | chr4.3:55116745-55116764 | MS.gene073244:CDS | 30.0% | |
| ! | CTTTTTATCTTGGGTCTTTT+AGG | - | chr4.3:55116747-55116766 | None:intergenic | 30.0% |
| ! | GAGAAAGATTTTCCTTTGTT+TGG | - | chr4.3:55116682-55116701 | None:intergenic | 30.0% |
| !!! | GTTTTGTTGTTTATGAGAAC+AGG | + | chr4.3:55116801-55116820 | MS.gene073244:CDS | 30.0% |
| ATGAGCTTCTTGGTTTACAA+GGG | + | chr4.3:55116613-55116632 | MS.gene073244:CDS | 35.0% | |
| GAAAGAGAAAACACTCAAGT+CGG | + | chr4.3:55116635-55116654 | MS.gene073244:CDS | 35.0% | |
| TATGAGCTTCTTGGTTTACA+AGG | + | chr4.3:55116612-55116631 | MS.gene073244:CDS | 35.0% | |
| ! | AACAGCAACCCTTTTTATCT+TGG | - | chr4.3:55116757-55116776 | None:intergenic | 35.0% |
| ! | ACAGCAACCCTTTTTATCTT+GGG | - | chr4.3:55116756-55116775 | None:intergenic | 35.0% |
| ! | GATAAAAAGGGTTGCTGTTA+AGG | + | chr4.3:55116758-55116777 | MS.gene073244:CDS | 35.0% |
| AGACAAGGTTATGAGCTTCT+TGG | + | chr4.3:55116603-55116622 | MS.gene073244:CDS | 40.0% | |
| AGGATCTTCCTAATAGGAAC+AGG | - | chr4.3:55116873-55116892 | None:intergenic | 40.0% | |
| ATAACCTTGTCTCCTGCTGA+AGG | - | chr4.3:55116595-55116614 | None:intergenic | 45.0% | |
| ! | CTAGCTCTCCTGTTCCTATT+AGG | + | chr4.3:55116862-55116881 | MS.gene073244:CDS | 45.0% |
| GACGCAGAAGAGCCAAACAA+AGG | + | chr4.3:55116667-55116686 | MS.gene073244:CDS | 50.0% | |
| !! | GAGAGTGGTGTTGCGAACAA+GGG | - | chr4.3:55116571-55116590 | None:intergenic | 50.0% |
| !! | TGAGAGTGGTGTTGCGAACA+AGG | - | chr4.3:55116572-55116591 | None:intergenic | 50.0% |
| ACACCACTCTCACCTTCAGC+AGG | + | chr4.3:55116580-55116599 | MS.gene073244:CDS | 55.0% | |
| CTCACCTTCAGCAGGAGACA+AGG | + | chr4.3:55116588-55116607 | MS.gene073244:CDS | 55.0% | |
| TCTCCTGCTGAAGGTGAGAG+TGG | - | chr4.3:55116586-55116605 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 55116561 | 55116896 | 55116561 | ID=MS.gene073244 |
| chr4.3 | mRNA | 55116561 | 55116896 | 55116561 | ID=MS.gene073244.t1;Parent=MS.gene073244 |
| chr4.3 | exon | 55116561 | 55116896 | 55116561 | ID=MS.gene073244.t1.exon1;Parent=MS.gene073244.t1 |
| chr4.3 | CDS | 55116561 | 55116896 | 55116561 | ID=cds.MS.gene073244.t1;Parent=MS.gene073244.t1 |
| Gene Sequence |
| Protein sequence |
