Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08680.t1 | RHN60701.1 | 94.6 | 129 | 7 | 0 | 1 | 129 | 538 | 666 | 5.70E-61 | 243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08680.t1 | A0A396I7I3 | 94.6 | 129 | 7 | 0 | 1 | 129 | 538 | 666 | 4.1e-61 | 243.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049647 | MS.gene08680 | 0.805169 | 1.52E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene08680 | 0.800604 | 1.33E-48 | -1.69E-46 |
| MS.gene050135 | MS.gene08680 | 0.807055 | 6.09E-50 | -1.69E-46 |
| MS.gene050137 | MS.gene08680 | 0.981457 | 1.23E-152 | -1.69E-46 |
| MS.gene050156 | MS.gene08680 | 0.810133 | 1.34E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene08680 | 0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene051265 | MS.gene08680 | 0.80772 | 4.40E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene08680 | 0.806797 | 6.91E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene08680 | 0.800873 | 1.17E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene08680 | 0.808951 | 2.41E-50 | -1.69E-46 |
| MS.gene053641 | MS.gene08680 | 0.834624 | 2.69E-56 | -1.69E-46 |
| MS.gene054467 | MS.gene08680 | 0.807355 | 5.26E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene08680 | 0.806498 | 7.99E-50 | -1.69E-46 |
| MS.gene05468 | MS.gene08680 | 0.836916 | 7.07E-57 | -1.69E-46 |
| MS.gene054775 | MS.gene08680 | 0.810514 | 1.11E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene08680 | 0.805328 | 1.41E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene08680 | 0.823504 | 1.34E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene08680 | 0.809836 | 1.55E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene08680 | 0.80763 | 4.60E-50 | -1.69E-46 |
| MS.gene055926 | MS.gene08680 | 0.802895 | 4.52E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene08680 | 0.826447 | 2.70E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene08680 | 0.804554 | 2.04E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene08680 | 0.800069 | 1.71E-48 | -1.69E-46 |
| MS.gene058168 | MS.gene08680 | 0.809408 | 1.92E-50 | -1.69E-46 |
| MS.gene058829 | MS.gene08680 | 0.801767 | 7.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACTGTTGAATCCATATT+TGG | 0.216643 | 4.4:+45186942 | None:intergenic |
| TGATCAGATTACACCAAATA+TGG | 0.305163 | 4.4:-45186955 | MS.gene08680:CDS |
| TGAAAAGCAGTTTCAGTGTT+TGG | 0.318681 | 4.4:-45187078 | MS.gene08680:CDS |
| CAGTTTCAGTGTTTGGAAAT+GGG | 0.336837 | 4.4:-45187071 | MS.gene08680:CDS |
| GCAGTTTCAGTGTTTGGAAA+TGG | 0.338021 | 4.4:-45187072 | MS.gene08680:CDS |
| TTGGAAATGGGGTCATCTTC+AGG | 0.341648 | 4.4:-45187059 | MS.gene08680:CDS |
| GTAGACCTTAGACATCAATT+TGG | 0.356780 | 4.4:-45186819 | MS.gene08680:CDS |
| GCTCTCCAAATTGATGTCTA+AGG | 0.413976 | 4.4:+45186814 | None:intergenic |
| TGGATCCGTATCGCTGACTT+TGG | 0.419631 | 4.4:-45186898 | MS.gene08680:CDS |
| AGGCAATAACAATGTTGAAA+AGG | 0.450410 | 4.4:-45187039 | MS.gene08680:CDS |
| CTAATTAACCCACAAAATCA+TGG | 0.474328 | 4.4:+45186784 | None:intergenic |
| GGATCCGTATCGCTGACTTT+GGG | 0.487993 | 4.4:-45186897 | MS.gene08680:CDS |
| ATACACATGCTTGAATCAAA+AGG | 0.494309 | 4.4:-45187161 | None:intergenic |
| AAATATGGATTCAACAGTGA+TGG | 0.505789 | 4.4:-45186940 | MS.gene08680:CDS |
| AGTTTCAGTGTTTGGAAATG+GGG | 0.530029 | 4.4:-45187070 | MS.gene08680:CDS |
| GCAGCAACATCAGTTGCAGC+AGG | 0.539152 | 4.4:-45186844 | MS.gene08680:CDS |
| GATACGGATCCACGTTGATA+CGG | 0.551902 | 4.4:+45186909 | None:intergenic |
| AATATGGATTCAACAGTGAT+GGG | 0.554086 | 4.4:-45186939 | MS.gene08680:CDS |
| TTATTACAAACGAAGAGCAA+TGG | 0.584359 | 4.4:-45187016 | MS.gene08680:CDS |
| TTAATAATATTCAAGAGAAG+AGG | 0.603624 | 4.4:-45186992 | MS.gene08680:CDS |
| GAGACCCAAAGTCAGCGATA+CGG | 0.605617 | 4.4:+45186893 | None:intergenic |
| GCAACATCAGTTGCAGCAGG+AGG | 0.630480 | 4.4:-45186841 | MS.gene08680:CDS |
| CTGACTTTGGGTCTCAGACA+CGG | 0.667702 | 4.4:-45186885 | MS.gene08680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAACAATGATAAAAATG+AGG | - | chr4.4:45186823-45186842 | MS.gene08680:CDS | 20.0% |
| !! | TTAATAATATTCAAGAGAAG+AGG | - | chr4.4:45186951-45186970 | MS.gene08680:CDS | 20.0% |
| AAATATGGATTCAACAGTGA+TGG | - | chr4.4:45187003-45187022 | MS.gene08680:CDS | 30.0% | |
| AATATGGATTCAACAGTGAT+GGG | - | chr4.4:45187004-45187023 | MS.gene08680:CDS | 30.0% | |
| AGGCAATAACAATGTTGAAA+AGG | - | chr4.4:45186904-45186923 | MS.gene08680:CDS | 30.0% | |
| ATCACTGTTGAATCCATATT+TGG | + | chr4.4:45187004-45187023 | None:intergenic | 30.0% | |
| TGATCAGATTACACCAAATA+TGG | - | chr4.4:45186988-45187007 | MS.gene08680:CDS | 30.0% | |
| TTATTACAAACGAAGAGCAA+TGG | - | chr4.4:45186927-45186946 | MS.gene08680:CDS | 30.0% | |
| AGTTTCAGTGTTTGGAAATG+GGG | - | chr4.4:45186873-45186892 | MS.gene08680:CDS | 35.0% | |
| CAGTTTCAGTGTTTGGAAAT+GGG | - | chr4.4:45186872-45186891 | MS.gene08680:CDS | 35.0% | |
| GTAGACCTTAGACATCAATT+TGG | - | chr4.4:45187124-45187143 | MS.gene08680:CDS | 35.0% | |
| TGAAAAGCAGTTTCAGTGTT+TGG | - | chr4.4:45186865-45186884 | MS.gene08680:CDS | 35.0% | |
| ! | CACATGATCCATGATTTTGT+GGG | - | chr4.4:45187151-45187170 | MS.gene08680:CDS | 35.0% |
| AAAAATGAGGCATGCAGAGT+CGG | - | chr4.4:45186836-45186855 | MS.gene08680:CDS | 40.0% | |
| GCAGTTTCAGTGTTTGGAAA+TGG | - | chr4.4:45186871-45186890 | MS.gene08680:CDS | 40.0% | |
| GCTCTCCAAATTGATGTCTA+AGG | + | chr4.4:45187132-45187151 | None:intergenic | 40.0% | |
| ! | GCACATGATCCATGATTTTG+TGG | - | chr4.4:45187150-45187169 | MS.gene08680:CDS | 40.0% |
| GATACGGATCCACGTTGATA+CGG | + | chr4.4:45187037-45187056 | None:intergenic | 45.0% | |
| TTGGAAATGGGGTCATCTTC+AGG | - | chr4.4:45186884-45186903 | MS.gene08680:CDS | 45.0% | |
| !! | GGTTTTATGCCGTATCAACG+TGG | - | chr4.4:45187025-45187044 | MS.gene08680:CDS | 45.0% |
| CTGACTTTGGGTCTCAGACA+CGG | - | chr4.4:45187058-45187077 | MS.gene08680:CDS | 50.0% | |
| GAGACCCAAAGTCAGCGATA+CGG | + | chr4.4:45187053-45187072 | None:intergenic | 50.0% | |
| !! | GGATCCGTATCGCTGACTTT+GGG | - | chr4.4:45187046-45187065 | MS.gene08680:CDS | 50.0% |
| !! | TGGATCCGTATCGCTGACTT+TGG | - | chr4.4:45187045-45187064 | MS.gene08680:CDS | 50.0% |
| GCAACATCAGTTGCAGCAGG+AGG | - | chr4.4:45187102-45187121 | MS.gene08680:CDS | 55.0% | |
| GCAGCAACATCAGTTGCAGC+AGG | - | chr4.4:45187099-45187118 | MS.gene08680:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 45186788 | 45187177 | 45186788 | ID=MS.gene08680 |
| chr4.4 | mRNA | 45186788 | 45187177 | 45186788 | ID=MS.gene08680.t1;Parent=MS.gene08680 |
| chr4.4 | exon | 45186788 | 45187177 | 45186788 | ID=MS.gene08680.t1.exon1;Parent=MS.gene08680.t1 |
| chr4.4 | CDS | 45186788 | 45187177 | 45186788 | ID=cds.MS.gene08680.t1;Parent=MS.gene08680.t1 |
| Gene Sequence |
| Protein sequence |
