AlfalfaGEDB | MS.gene050137

M. sativa cultivar XinJiangDaYe / MS.gene050137

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050137.t1	RHN60701.1	94.6	129	7	0	1	129	538	666	5.70E-61	243.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050137.t1	A0A396I7I3	94.6	129	7	0	1	129	538	666	4.1e-61	243.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050136	MS.gene050137	0.833352	5.60E-56	-1.69E-46
MS.gene050137	MS.gene050156	0.800348	1.50E-48	-1.69E-46
MS.gene050137	MS.gene051247	0.813109	3.02E-51	-1.69E-46
MS.gene050137	MS.gene051265	0.804087	2.56E-49	-1.69E-46
MS.gene050137	MS.gene053641	0.82084	5.55E-53	-1.69E-46
MS.gene050137	MS.gene05468	0.831796	1.36E-55	-1.69E-46
MS.gene050137	MS.gene055356	0.805811	1.11E-49	-1.69E-46
MS.gene050137	MS.gene055357	0.810807	9.59E-51	-1.69E-46
MS.gene050137	MS.gene055358	0.801326	9.49E-49	-1.69E-46
MS.gene050137	MS.gene05604	0.826325	2.89E-54	-1.69E-46
MS.gene050137	MS.gene061333	0.821938	3.09E-53	-1.69E-46
MS.gene050137	MS.gene061334	0.814702	1.35E-51	-1.69E-46
MS.gene050137	MS.gene061336	0.803528	3.34E-49	-1.69E-46
MS.gene050137	MS.gene061337	0.815375	9.55E-52	-1.69E-46
MS.gene050137	MS.gene062743	0.802297	6.00E-49	-1.69E-46
MS.gene050137	MS.gene06339	0.800127	1.67E-48	-1.69E-46
MS.gene050137	MS.gene064944	0.827414	1.59E-54	-1.69E-46
MS.gene050137	MS.gene066320	0.813157	2.95E-51	-1.69E-46
MS.gene050137	MS.gene066782	0.807993	3.85E-50	-1.69E-46
MS.gene050137	MS.gene066784	0.80888	2.49E-50	-1.69E-46
MS.gene050137	MS.gene068609	0.815723	7.99E-52	-1.69E-46
MS.gene050137	MS.gene071457	0.800629	1.32E-48	-1.69E-46
MS.gene050137	MS.gene07344	0.831081	2.04E-55	-1.69E-46
MS.gene050137	MS.gene073753	0.814027	1.90E-51	-1.69E-46
MS.gene050137	MS.gene07876	0.804614	1.99E-49	-1.69E-46
MS.gene050137	MS.gene08680	0.981457	1.23E-152	-1.69E-46
MS.gene050137	MS.gene08743	0.865082	7.64E-65	-1.69E-46
MS.gene050137	MS.gene09740	0.801609	8.31E-49	-1.69E-46
MS.gene050137	MS.gene20809	0.802073	6.67E-49	-1.69E-46
MS.gene050137	MS.gene21927	0.809192	2.14E-50	-1.69E-46
MS.gene050137	MS.gene21929	0.809473	1.86E-50	-1.69E-46
MS.gene050137	MS.gene22271	0.807398	5.15E-50	-1.69E-46
MS.gene050137	MS.gene23571	0.813521	2.45E-51	-1.69E-46
MS.gene050137	MS.gene25706	0.800328	1.52E-48	-1.69E-46
MS.gene050137	MS.gene25872	0.807584	4.71E-50	-1.69E-46
MS.gene050137	MS.gene27414	0.83693	7.01E-57	-1.69E-46
MS.gene050137	MS.gene29022	0.803161	3.98E-49	-1.69E-46
MS.gene050137	MS.gene29095	0.802234	6.18E-49	-1.69E-46
MS.gene050137	MS.gene30350	0.814205	1.73E-51	-1.69E-46
MS.gene050137	MS.gene31360	0.80157	8.46E-49	-1.69E-46
MS.gene050137	MS.gene33527	0.809473	1.86E-50	-1.69E-46
MS.gene050137	MS.gene36899	0.823937	1.06E-53	-1.69E-46
MS.gene050137	MS.gene37338	0.803311	3.70E-49	-1.69E-46
MS.gene050137	MS.gene40837	-0.809956	1.46E-50	-1.69E-46
MS.gene050137	MS.gene48577	0.806925	6.49E-50	-1.69E-46
MS.gene050137	MS.gene53321	0.835404	1.71E-56	-1.69E-46
MS.gene050137	MS.gene53586	0.833402	5.45E-56	-1.69E-46
MS.gene050137	MS.gene55406	0.802917	4.47E-49	-1.69E-46
MS.gene050137	MS.gene56748	0.82696	2.04E-54	-1.69E-46
MS.gene050137	MS.gene57486	0.817809	2.72E-52	-1.69E-46
MS.gene050137	MS.gene58468	0.809432	1.90E-50	-1.69E-46
MS.gene050137	MS.gene58547	0.809168	2.16E-50	-1.69E-46
MS.gene050137	MS.gene58802	0.811785	5.88E-51	-1.69E-46
MS.gene050137	MS.gene59840	0.848576	5.61E-60	-1.69E-46
MS.gene050137	MS.gene59848	0.804008	2.66E-49	-1.69E-46
MS.gene050137	MS.gene62214	0.802416	5.67E-49	-1.69E-46
MS.gene050137	MS.gene64249	0.827121	1.86E-54	-1.69E-46
MS.gene050137	MS.gene65172	0.822857	1.89E-53	-1.69E-46
MS.gene050137	MS.gene70022	0.800778	1.23E-48	-1.69E-46
MS.gene050137	MS.gene72268	0.834983	2.19E-56	-1.69E-46
MS.gene050137	MS.gene72812	0.802994	4.31E-49	-1.69E-46
MS.gene050137	MS.gene73043	0.801279	9.71E-49	-1.69E-46
MS.gene050137	MS.gene73250	0.83425	3.34E-56	-1.69E-46
MS.gene050137	MS.gene73902	0.802095	6.60E-49	-1.69E-46
MS.gene050137	MS.gene74367	0.845377	4.22E-59	-1.69E-46
MS.gene050137	MS.gene74370	0.805825	1.11E-49	-1.69E-46
MS.gene050137	MS.gene74373	0.805849	1.09E-49	-1.69E-46
MS.gene050137	MS.gene77453	0.801335	9.45E-49	-1.69E-46
MS.gene050137	MS.gene77781	0.854275	1.37E-61	-1.69E-46
MS.gene050137	MS.gene77785	0.801267	9.76E-49	-1.69E-46
MS.gene050137	MS.gene79919	0.803631	3.18E-49	-1.69E-46
MS.gene050137	MS.gene79960	0.803783	2.96E-49	-1.69E-46
MS.gene050137	MS.gene80853	0.804561	2.04E-49	-1.69E-46
MS.gene050137	MS.gene81370	0.802331	5.90E-49	-1.69E-46
MS.gene050137	MS.gene82321	0.812042	5.17E-51	-1.69E-46
MS.gene050137	MS.gene85553	0.811488	6.83E-51	-1.69E-46
MS.gene050137	MS.gene86086	0.805241	1.47E-49	-1.69E-46
MS.gene050137	MS.gene86335	0.804904	1.73E-49	-1.69E-46
MS.gene050137	MS.gene88243	0.809564	1.78E-50	-1.69E-46
MS.gene050137	MS.gene90649	0.811014	8.65E-51	-1.69E-46
MS.gene050137	MS.gene91807	0.803652	3.15E-49	-1.69E-46
MS.gene050137	MS.gene91808	0.80003	1.74E-48	-1.69E-46
MS.gene050137	MS.gene91809	0.828221	1.01E-54	-1.69E-46
MS.gene050137	MS.gene92495	0.806657	7.39E-50	-1.69E-46
MS.gene050137	MS.gene92579	0.810004	1.43E-50	-1.69E-46
MS.gene050137	MS.gene95236	0.801659	8.11E-49	-1.69E-46
MS.gene050137	MS.gene98848	0.814984	1.17E-51	-1.69E-46
MS.gene050137	MS.gene99254	0.809552	1.79E-50	-1.69E-46
MS.gene050137	MS.gene99849	0.803892	2.81E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCACTGTTGAATCCATATT+TGG	0.216643	4.1:+39730465	None:intergenic
TGATCAGATTACACCAAATA+TGG	0.305163	4.1:-39730478	MS.gene050137:CDS
TGAAAAGCAGTTTCAGTGTT+TGG	0.318681	4.1:-39730601	MS.gene050137:CDS
CAGTTTCAGTGTTTGGAAAT+GGG	0.336837	4.1:-39730594	MS.gene050137:CDS
GCAGTTTCAGTGTTTGGAAA+TGG	0.338021	4.1:-39730595	MS.gene050137:CDS
TTGGAAATGGGGTCATCTTC+AGG	0.341648	4.1:-39730582	MS.gene050137:CDS
GTAGACCTTAGACATCAATT+TGG	0.356780	4.1:-39730342	MS.gene050137:CDS
GCTCTCCAAATTGATGTCTA+AGG	0.413976	4.1:+39730337	None:intergenic
TGGATCCGTATCGCTGACTT+TGG	0.419631	4.1:-39730421	MS.gene050137:CDS
AGGCAATAACAATGTTGAAA+AGG	0.450410	4.1:-39730562	MS.gene050137:CDS
CTAATTAACCCACAAAATCA+TGG	0.474328	4.1:+39730307	None:intergenic
GGATCCGTATCGCTGACTTT+GGG	0.487993	4.1:-39730420	MS.gene050137:CDS
ATACACATGCTTGAATCAAA+AGG	0.494309	4.1:-39730684	None:intergenic
AAATATGGATTCAACAGTGA+TGG	0.505789	4.1:-39730463	MS.gene050137:CDS
AGTTTCAGTGTTTGGAAATG+GGG	0.530029	4.1:-39730593	MS.gene050137:CDS
GCAGCAACATCAGTTGCAGC+AGG	0.539152	4.1:-39730367	MS.gene050137:CDS
GATACGGATCCACGTTGATA+CGG	0.551902	4.1:+39730432	None:intergenic
AATATGGATTCAACAGTGAT+GGG	0.554086	4.1:-39730462	MS.gene050137:CDS
TTATTACAAACGAAGAGCAA+TGG	0.584359	4.1:-39730539	MS.gene050137:CDS
TTAATAATATTCAAGAGAAG+AGG	0.603624	4.1:-39730515	MS.gene050137:CDS
GAGACCCAAAGTCAGCGATA+CGG	0.605617	4.1:+39730416	None:intergenic
GCAACATCAGTTGCAGCAGG+AGG	0.630480	4.1:-39730364	MS.gene050137:CDS
CTGACTTTGGGTCTCAGACA+CGG	0.667702	4.1:-39730408	MS.gene050137:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCAAAACAATGATAAAAATG+AGG	-	chr4.1:39730346-39730365	MS.gene050137:CDS	20.0%
!!	TTAATAATATTCAAGAGAAG+AGG	-	chr4.1:39730474-39730493	MS.gene050137:CDS	20.0%
	AAATATGGATTCAACAGTGA+TGG	-	chr4.1:39730526-39730545	MS.gene050137:CDS	30.0%
	AATATGGATTCAACAGTGAT+GGG	-	chr4.1:39730527-39730546	MS.gene050137:CDS	30.0%
	AGGCAATAACAATGTTGAAA+AGG	-	chr4.1:39730427-39730446	MS.gene050137:CDS	30.0%
	ATCACTGTTGAATCCATATT+TGG	+	chr4.1:39730527-39730546	None:intergenic	30.0%
	TGATCAGATTACACCAAATA+TGG	-	chr4.1:39730511-39730530	MS.gene050137:CDS	30.0%
	TTATTACAAACGAAGAGCAA+TGG	-	chr4.1:39730450-39730469	MS.gene050137:CDS	30.0%
	AGTTTCAGTGTTTGGAAATG+GGG	-	chr4.1:39730396-39730415	MS.gene050137:CDS	35.0%
	CAGTTTCAGTGTTTGGAAAT+GGG	-	chr4.1:39730395-39730414	MS.gene050137:CDS	35.0%
	GTAGACCTTAGACATCAATT+TGG	-	chr4.1:39730647-39730666	MS.gene050137:CDS	35.0%
	TGAAAAGCAGTTTCAGTGTT+TGG	-	chr4.1:39730388-39730407	MS.gene050137:CDS	35.0%
!	CACATGATCCATGATTTTGT+GGG	-	chr4.1:39730674-39730693	MS.gene050137:CDS	35.0%
	AAAAATGAGGCATGCAGAGT+CGG	-	chr4.1:39730359-39730378	MS.gene050137:CDS	40.0%
	GCAGTTTCAGTGTTTGGAAA+TGG	-	chr4.1:39730394-39730413	MS.gene050137:CDS	40.0%
	GCTCTCCAAATTGATGTCTA+AGG	+	chr4.1:39730655-39730674	None:intergenic	40.0%
!	GCACATGATCCATGATTTTG+TGG	-	chr4.1:39730673-39730692	MS.gene050137:CDS	40.0%
	GATACGGATCCACGTTGATA+CGG	+	chr4.1:39730560-39730579	None:intergenic	45.0%
	TTGGAAATGGGGTCATCTTC+AGG	-	chr4.1:39730407-39730426	MS.gene050137:CDS	45.0%
!!	GGTTTTATGCCGTATCAACG+TGG	-	chr4.1:39730548-39730567	MS.gene050137:CDS	45.0%
	CTGACTTTGGGTCTCAGACA+CGG	-	chr4.1:39730581-39730600	MS.gene050137:CDS	50.0%
	GAGACCCAAAGTCAGCGATA+CGG	+	chr4.1:39730576-39730595	None:intergenic	50.0%
!!	GGATCCGTATCGCTGACTTT+GGG	-	chr4.1:39730569-39730588	MS.gene050137:CDS	50.0%
!!	TGGATCCGTATCGCTGACTT+TGG	-	chr4.1:39730568-39730587	MS.gene050137:CDS	50.0%
	GCAACATCAGTTGCAGCAGG+AGG	-	chr4.1:39730625-39730644	MS.gene050137:CDS	55.0%
	GCAGCAACATCAGTTGCAGC+AGG	-	chr4.1:39730622-39730641	MS.gene050137:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	39730311	39730700	39730311	ID=MS.gene050137
chr4.1	mRNA	39730311	39730700	39730311	ID=MS.gene050137.t1;Parent=MS.gene050137
chr4.1	exon	39730311	39730700	39730311	ID=MS.gene050137.t1.exon1;Parent=MS.gene050137.t1
chr4.1	CDS	39730311	39730700	39730311	ID=cds.MS.gene050137.t1;Parent=MS.gene050137.t1

Gene Sequence

>MS.gene050137

ATGCTTGAATCAAAAGGCACAGCAGAAGCTCAGAATCAAAACAATGATAAAAATGAGGCATGCAGAGTCGGAACGCATGAAAAGCAGTTTCAGTGTTTGGAAATGGGGTCATCTTCAGGCAATAACAATGTTGAAAAGGTTATTACAAACGAAGAGCAATGGATTAATAATATTCAAGAGAAGAGGTCTAAATTAGAAAGTGATCAGATTACACCAAATATGGATTCAACAGTGATGGGTTTTATGCCGTATCAACGTGGATCCGTATCGCTGACTTTGGGTCTCAGACACGGCGTTGAAAGTGTACAACAGCAGCAACATCAGTTGCAGCAGGAGGTAGACCTTAGACATCAATTTGGAGAGCACATGATCCATGATTTTGTGGGTTAA

Protein sequence

>MS.gene050137.t1

MLESKGTAEAQNQNNDKNEACRVGTHEKQFQCLEMGSSSGNNNVEKVITNEEQWINNIQEKRSKLESDQITPNMDSTVMGFMPYQRGSVSLTLGLRHGVESVQQQQHQLQQEVDLRHQFGEHMIHDFVG