AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08783


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08783.t1 MTR_8g085590 93.789 161 7 1 1 158 1 161 5.66e-98 279
MS.gene08783.t1 MTR_8g085580 41.270 126 72 2 23 147 22 146 1.54e-30 108
MS.gene08783.t1 MTR_4g081560 41.026 117 67 2 31 146 29 144 3.19e-27 100
MS.gene08783.t1 MTR_7g080280 37.778 135 77 4 18 150 32 161 4.91e-26 97.4
MS.gene08783.t1 MTR_7g053107 35.088 114 72 2 31 143 36 148 3.51e-22 87.8
MS.gene08783.t1 MTR_2g010970 34.188 117 75 2 31 146 28 143 1.29e-21 85.9
MS.gene08783.t1 MTR_4g091980 31.111 135 91 2 12 145 12 145 1.58e-20 83.2
MS.gene08783.t1 MTR_2g010970 35.227 88 56 1 31 118 28 114 3.36e-17 73.6
MS.gene08783.t1 MTR_4g091930 28.148 135 95 2 12 145 12 145 3.89e-17 73.9
MS.gene08783.t1 MTR_4g091970 27.632 152 105 4 1 150 3 151 2.33e-16 72.4
MS.gene08783.t1 MTR_4g064460 29.661 118 81 2 29 145 29 145 1.06e-15 70.5
MS.gene08783.t1 MTR_6g086680 30.534 131 85 3 23 149 27 155 2.21e-14 66.6
MS.gene08783.t1 MTR_7g108775 29.008 131 88 3 16 143 14 142 2.81e-14 66.6
MS.gene08783.t1 MTR_7g108775 29.008 131 88 3 16 143 14 142 4.85e-14 66.6
MS.gene08783.t1 MTR_1g088250 35.652 115 68 4 32 143 25 136 8.22e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08783.t1 AT1G55265 60.000 115 46 0 30 144 53 167 1.61e-46 149
MS.gene08783.t1 AT5G19860 41.379 116 66 2 29 143 34 148 9.66e-27 99.8
MS.gene08783.t1 AT1G61667 38.462 117 69 3 29 143 20 135 1.09e-25 96.3
MS.gene08783.t1 AT1G61667 38.462 117 69 3 29 143 56 171 1.45e-25 96.7
MS.gene08783.t1 AT5G54530 38.261 115 69 2 31 144 29 142 1.83e-24 93.2
MS.gene08783.t1 AT3G07470 29.747 158 108 3 1 156 1 157 2.52e-20 82.4
MS.gene08783.t1 AT3G07470 31.034 145 97 3 1 143 1 144 1.76e-19 80.1
MS.gene08783.t1 AT3G07470 31.034 145 97 3 1 143 1 144 2.12e-19 80.1
MS.gene08783.t1 AT5G16380 31.757 148 93 6 12 156 14 156 4.30e-18 77.4
MS.gene08783.t1 AT3G07460 29.927 137 93 3 19 153 18 153 6.68e-18 76.6
MS.gene08783.t1 AT3G07460 29.927 137 93 3 19 153 18 153 3.77e-17 76.3
MS.gene08783.t1 AT1G02813 28.467 137 95 2 8 143 2 136 1.11e-16 72.8
MS.gene08783.t1 AT4G02360 28.030 132 91 3 13 143 12 140 9.06e-14 65.1
MS.gene08783.t1 AT1G02816 32.609 138 84 5 12 143 10 144 1.07e-13 65.5
MS.gene08783.t1 AT2G03350 36.607 112 65 5 33 141 61 169 1.78e-13 65.1
MS.gene08783.t1 AT5G19590 30.159 126 83 3 28 150 28 151 1.68e-12 61.6
MS.gene08783.t1 AT5G19590 30.159 126 83 3 28 150 28 151 1.68e-12 61.6

Find 63 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCACTTACGGTTCCGTTTC+CGG 0.165426 4.2:-12828340 MS.gene08783:CDS
TTCATCTGGCTCCCTGTTAC+TGG 0.204778 4.2:-12828289 MS.gene08783:CDS
TTCCGGTGTTTCCGGGATTC+AGG 0.260942 4.2:-12828323 MS.gene08783:CDS
TCAACTTCCATTCCAGTAAC+AGG 0.329362 4.2:+12828277 None:intergenic
GGTTCCGTTTCCGGTGTTTC+CGG 0.329438 4.2:-12828331 MS.gene08783:CDS
ATGGAAGTTGATTCCCGTTC+TGG 0.333675 4.2:-12828265 MS.gene08783:CDS
GTTCCGTTTCCGGTGTTTCC+GGG 0.340302 4.2:-12828330 MS.gene08783:CDS
ACCGGAGAAGCGAACGTAAC+AGG 0.340745 4.2:+12828399 None:intergenic
GGTATGCTTCAATTCTTTGT+TGG 0.346268 4.2:-12828244 MS.gene08783:CDS
GTGTAGGAGGCGATGTTGTT+AGG 0.359883 4.2:+12828463 None:intergenic
TTCTGCAAGTAAATACTTTG+TGG 0.366443 4.2:-12828158 MS.gene08783:CDS
CCCTGTTACGTTCGCTTCTC+CGG 0.394931 4.2:-12828400 MS.gene08783:CDS
TGTTAGGAAGGATGCCCTTT+GGG 0.399077 4.2:+12828479 None:intergenic
GCCCTTTGGGAAACCGTAAT+CGG 0.407957 4.2:+12828492 None:intergenic
AGCTTTGTCTAAGAAATTAC+CGG 0.420637 4.2:-12828221 MS.gene08783:CDS
CCCTTTGGGAAACCGTAATC+GGG 0.430500 4.2:+12828493 None:intergenic
CACGACCTCCTCCCCGATTA+CGG 0.436117 4.2:-12828505 MS.gene08783:CDS
TCCTACACCCTCTCCTCATC+CGG 0.443813 4.2:-12828448 MS.gene08783:CDS
AGGCCAAGATGCTCTTCATC+TGG 0.445884 4.2:-12828303 MS.gene08783:CDS
GAAGGGTTGTGGCTGTTGCA+TGG 0.449955 4.2:+12828539 None:intergenic
GAATTGAAGCATACCAGAAC+GGG 0.452831 4.2:+12828252 None:intergenic
CCCGATTACGGTTTCCCAAA+GGG 0.457567 4.2:-12828493 MS.gene08783:CDS
TTGTTAGGAAGGATGCCCTT+TGG 0.469698 4.2:+12828478 None:intergenic
CGTAATCGGGGAGGAGGTCG+TGG 0.480861 4.2:+12828506 None:intergenic
TACTACGACACCCTCGTCAC+CGG 0.484561 4.2:-12828367 MS.gene08783:CDS
AATCCCGGAAACACCGGAAA+CGG 0.488780 4.2:+12828327 None:intergenic
CTGGCTCCCTGTTACTGGAA+TGG 0.520106 4.2:-12828284 MS.gene08783:CDS
CTGTTGCATGGGATATGAAT+AGG 0.520381 4.2:+12828551 None:intergenic
GAGATGGAGGGTGAAGTAAC+CGG 0.520551 4.2:+12828429 None:intergenic
CCGGAGAAGCGAACGTAACA+GGG 0.524118 4.2:+12828400 None:intergenic
AGGAGGCGATGTTGTTAGGA+AGG 0.525908 4.2:+12828467 None:intergenic
AGGAGGTCGTGGATGTCCGA+TGG 0.528432 4.2:+12828517 None:intergenic
GAGCATCTTGGCCTGAATCC+CGG 0.529909 4.2:+12828312 None:intergenic
GAGGGTGAAGTAACCGGATG+AGG 0.538161 4.2:+12828435 None:intergenic
GGAAACCGTAATCGGGGAGG+AGG 0.542068 4.2:+12828500 None:intergenic
GGTCGTGGATGTCCGATGGA+AGG 0.542935 4.2:+12828521 None:intergenic
GAGCCAGATGAAGAGCATCT+TGG 0.544202 4.2:+12828300 None:intergenic
AAGGGTTGTGGCTGTTGCAT+GGG 0.549396 4.2:+12828540 None:intergenic
CCCCGATTACGGTTTCCCAA+AGG 0.549824 4.2:-12828494 MS.gene08783:CDS
AATCAGTTTCAAGATGTGCC+TGG 0.550036 4.2:-12828196 MS.gene08783:CDS
AAGTGAGGGTTCCGGTGACG+AGG 0.556293 4.2:+12828356 None:intergenic
GTCGTGGATGTCCGATGGAA+GGG 0.557395 4.2:+12828522 None:intergenic
GGCCTGAATCCCGGAAACAC+CGG 0.568473 4.2:+12828321 None:intergenic
ACATCTTGAAACTGATTCGC+CGG 0.569152 4.2:+12828202 None:intergenic
GTCACCGGAACCCTCACTTA+CGG 0.574574 4.2:-12828352 MS.gene08783:CDS
TGTTGAAAAGAATTGAAACA+AGG 0.578826 4.2:+12828576 None:intergenic
ACCGGATGAGGAGAGGGTGT+AGG 0.581276 4.2:+12828447 None:intergenic
GTCGTAGTAGACTAATTGAC+CGG 0.588836 4.2:+12828381 None:intergenic
GAAGTAACCGGATGAGGAGA+GGG 0.591167 4.2:+12828441 None:intergenic
CGGAAACGGAACCGTAAGTG+AGG 0.593119 4.2:+12828341 None:intergenic
GGATGAGGAGAGGGTGTAGG+AGG 0.593846 4.2:+12828450 None:intergenic
GGAACCGTAAGTGAGGGTTC+CGG 0.598341 4.2:+12828348 None:intergenic
AACAGCCACAACCCTTCCAT+CGG 0.601184 4.2:-12828533 MS.gene08783:CDS
AGAATTGAAGCATACCAGAA+CGG 0.613753 4.2:+12828251 None:intergenic
TGAAGTAACCGGATGAGGAG+AGG 0.621652 4.2:+12828440 None:intergenic
CAACTTCCATTCCAGTAACA+GGG 0.628240 4.2:+12828278 None:intergenic
AGTGAGGGTTCCGGTGACGA+GGG 0.629622 4.2:+12828357 None:intergenic
GATGTCCGATGGAAGGGTTG+TGG 0.630291 4.2:+12828528 None:intergenic
TTGGGAAACCGTAATCGGGG+AGG 0.667430 4.2:+12828497 None:intergenic
CCTTTGGGAAACCGTAATCG+GGG 0.689281 4.2:+12828494 None:intergenic
CGGAGAAGCGAACGTAACAG+GGG 0.693110 4.2:+12828401 None:intergenic
GGAAACGGAACCGTAAGTGA+GGG 0.696303 4.2:+12828342 None:intergenic
GGAGAAGCGAACGTAACAGG+GGG 0.736155 4.2:+12828402 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTTGAAAAGAATTGAAACA+AGG + chr4.2:12828169-12828188 None:intergenic 25.0%
TTCTGCAAGTAAATACTTTG+TGG - chr4.2:12828584-12828603 MS.gene08783:CDS 30.0%
! AGCTTTGTCTAAGAAATTAC+CGG - chr4.2:12828521-12828540 MS.gene08783:CDS 30.0%
! GAATTGAAACAAGGTTTTCA+AGG + chr4.2:12828160-12828179 None:intergenic 30.0%
AGAATTGAAGCATACCAGAA+CGG + chr4.2:12828494-12828513 None:intergenic 35.0%
GGTATGCTTCAATTCTTTGT+TGG - chr4.2:12828498-12828517 MS.gene08783:CDS 35.0%
!! AAACAAGGTTTTCAAGGTTC+TGG + chr4.2:12828154-12828173 None:intergenic 35.0%
!! GAAGTTTTTGAAGAACATCC+AGG + chr4.2:12828567-12828586 None:intergenic 35.0%
AATCAGTTTCAAGATGTGCC+TGG - chr4.2:12828546-12828565 MS.gene08783:CDS 40.0%
CAACTTCCATTCCAGTAACA+GGG + chr4.2:12828467-12828486 None:intergenic 40.0%
CTGTTGCATGGGATATGAAT+AGG + chr4.2:12828194-12828213 None:intergenic 40.0%
GAATTGAAGCATACCAGAAC+GGG + chr4.2:12828493-12828512 None:intergenic 40.0%
GTCGTAGTAGACTAATTGAC+CGG + chr4.2:12828364-12828383 None:intergenic 40.0%
TCAACTTCCATTCCAGTAAC+AGG + chr4.2:12828468-12828487 None:intergenic 40.0%
!! ACATCTTGAAACTGATTCGC+CGG + chr4.2:12828543-12828562 None:intergenic 40.0%
TGTTAGGAAGGATGCCCTTT+GGG + chr4.2:12828266-12828285 None:intergenic 45.0%
TTGTTAGGAAGGATGCCCTT+TGG + chr4.2:12828267-12828286 None:intergenic 45.0%
! ATGGAAGTTGATTCCCGTTC+TGG - chr4.2:12828477-12828496 MS.gene08783:CDS 45.0%
!! CAAGGTTTTCAAGGTTCTGG+TGG + chr4.2:12828151-12828170 None:intergenic 45.0%
AACAGCCACAACCCTTCCAT+CGG - chr4.2:12828209-12828228 MS.gene08783:CDS 50.0%
AAGGGTTGTGGCTGTTGCAT+GGG + chr4.2:12828205-12828224 None:intergenic 50.0%
AATCCCGGAAACACCGGAAA+CGG + chr4.2:12828418-12828437 None:intergenic 50.0%
AGGAGGCGATGTTGTTAGGA+AGG + chr4.2:12828278-12828297 None:intergenic 50.0%
AGGCCAAGATGCTCTTCATC+TGG - chr4.2:12828439-12828458 MS.gene08783:CDS 50.0%
CCCGATTACGGTTTCCCAAA+GGG - chr4.2:12828249-12828268 MS.gene08783:CDS 50.0%
CTCACTTACGGTTCCGTTTC+CGG - chr4.2:12828402-12828421 MS.gene08783:CDS 50.0%
GAAGTAACCGGATGAGGAGA+GGG + chr4.2:12828304-12828323 None:intergenic 50.0%
GAGATGGAGGGTGAAGTAAC+CGG + chr4.2:12828316-12828335 None:intergenic 50.0%
GGAAACGGAACCGTAAGTGA+GGG + chr4.2:12828403-12828422 None:intergenic 50.0%
GTGTAGGAGGCGATGTTGTT+AGG + chr4.2:12828282-12828301 None:intergenic 50.0%
TGAAGTAACCGGATGAGGAG+AGG + chr4.2:12828305-12828324 None:intergenic 50.0%
! CCCTTTGGGAAACCGTAATC+GGG + chr4.2:12828252-12828271 None:intergenic 50.0%
! CCTTTGGGAAACCGTAATCG+GGG + chr4.2:12828251-12828270 None:intergenic 50.0%
! GCCCTTTGGGAAACCGTAAT+CGG + chr4.2:12828253-12828272 None:intergenic 50.0%
! TTCATCTGGCTCCCTGTTAC+TGG - chr4.2:12828453-12828472 MS.gene08783:CDS 50.0%
!! GAGCCAGATGAAGAGCATCT+TGG + chr4.2:12828445-12828464 None:intergenic 50.0%
AACAGGGGGATTGGAGATGG+AGG + chr4.2:12828329-12828348 None:intergenic 55.0%
ACAGGGGGATTGGAGATGGA+GGG + chr4.2:12828328-12828347 None:intergenic 55.0%
ACCGGAGAAGCGAACGTAAC+AGG + chr4.2:12828346-12828365 None:intergenic 55.0%
AGCGAACGTAACAGGGGGAT+TGG + chr4.2:12828338-12828357 None:intergenic 55.0%
CCCCGATTACGGTTTCCCAA+AGG - chr4.2:12828248-12828267 MS.gene08783:CDS 55.0%
CCCTGTTACGTTCGCTTCTC+CGG - chr4.2:12828342-12828361 MS.gene08783:CDS 55.0%
CCGGAGAAGCGAACGTAACA+GGG + chr4.2:12828345-12828364 None:intergenic 55.0%
CGGAAACGGAACCGTAAGTG+AGG + chr4.2:12828404-12828423 None:intergenic 55.0%
CGGAGAAGCGAACGTAACAG+GGG + chr4.2:12828344-12828363 None:intergenic 55.0%
CGTAACAGGGGGATTGGAGA+TGG + chr4.2:12828332-12828351 None:intergenic 55.0%
CTGGCTCCCTGTTACTGGAA+TGG - chr4.2:12828458-12828477 MS.gene08783:CDS 55.0%
GAAGGGTTGTGGCTGTTGCA+TGG + chr4.2:12828206-12828225 None:intergenic 55.0%
GAGCATCTTGGCCTGAATCC+CGG + chr4.2:12828433-12828452 None:intergenic 55.0%
GAGGGTGAAGTAACCGGATG+AGG + chr4.2:12828310-12828329 None:intergenic 55.0%
GATGTCCGATGGAAGGGTTG+TGG + chr4.2:12828217-12828236 None:intergenic 55.0%
GGAACCGTAAGTGAGGGTTC+CGG + chr4.2:12828397-12828416 None:intergenic 55.0%
GGAGAAGCGAACGTAACAGG+GGG + chr4.2:12828343-12828362 None:intergenic 55.0%
GGTTCCGTTTCCGGTGTTTC+CGG - chr4.2:12828411-12828430 MS.gene08783:CDS 55.0%
GTCACCGGAACCCTCACTTA+CGG - chr4.2:12828390-12828409 MS.gene08783:CDS 55.0%
GTCGTGGATGTCCGATGGAA+GGG + chr4.2:12828223-12828242 None:intergenic 55.0%
GTTCCGTTTCCGGTGTTTCC+GGG - chr4.2:12828412-12828431 MS.gene08783:CDS 55.0%
TACTACGACACCCTCGTCAC+CGG - chr4.2:12828375-12828394 MS.gene08783:CDS 55.0%
TCCTACACCCTCTCCTCATC+CGG - chr4.2:12828294-12828313 MS.gene08783:CDS 55.0%
TTCCGGTGTTTCCGGGATTC+AGG - chr4.2:12828419-12828438 MS.gene08783:CDS 55.0%
TTGGGAAACCGTAATCGGGG+AGG + chr4.2:12828248-12828267 None:intergenic 55.0%
AAGTGAGGGTTCCGGTGACG+AGG + chr4.2:12828389-12828408 None:intergenic 60.0%
AGGAGGTCGTGGATGTCCGA+TGG + chr4.2:12828228-12828247 None:intergenic 60.0%
AGTGAGGGTTCCGGTGACGA+GGG + chr4.2:12828388-12828407 None:intergenic 60.0%
CACGACCTCCTCCCCGATTA+CGG - chr4.2:12828237-12828256 MS.gene08783:CDS 60.0%
GGAAACCGTAATCGGGGAGG+AGG + chr4.2:12828245-12828264 None:intergenic 60.0%
GGCCTGAATCCCGGAAACAC+CGG + chr4.2:12828424-12828443 None:intergenic 60.0%
GGTCGTGGATGTCCGATGGA+AGG + chr4.2:12828224-12828243 None:intergenic 60.0%
! ACCGGATGAGGAGAGGGTGT+AGG + chr4.2:12828298-12828317 None:intergenic 60.0%
! GGATGAGGAGAGGGTGTAGG+AGG + chr4.2:12828295-12828314 None:intergenic 60.0%
CGTAATCGGGGAGGAGGTCG+TGG + chr4.2:12828239-12828258 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 12828144 12828620 12828144 ID=MS.gene08783
chr4.2 mRNA 12828144 12828620 12828144 ID=MS.gene08783.t1;Parent=MS.gene08783
chr4.2 exon 12828144 12828620 12828144 ID=MS.gene08783.t1.exon1;Parent=MS.gene08783.t1
chr4.2 CDS 12828144 12828620 12828144 ID=cds.MS.gene08783.t1;Parent=MS.gene08783.t1
Gene Sequence

>MS.gene08783

ATGTCCACCAGAACCTTGAAAACCTTGTTTCAATTCTTTTCAACATTCCTATTCATATCCCATGCAACAGCCACAACCCTTCCATCGGACATCCACGACCTCCTCCCCGATTACGGTTTCCCAAAGGGCATCCTTCCTAACAACATCGCCTCCTACACCCTCTCCTCATCCGGTTACTTCACCCTCCATCTCCAATCCCCCTGTTACGTTCGCTTCTCCGGTCAATTAGTCTACTACGACACCCTCGTCACCGGAACCCTCACTTACGGTTCCGTTTCCGGTGTTTCCGGGATTCAGGCCAAGATGCTCTTCATCTGGCTCCCTGTTACTGGAATGGAAGTTGATTCCCGTTCTGGTATGCTTCAATTCTTTGTTGGAGCTTTGTCTAAGAAATTACCGGCGAATCAGTTTCAAGATGTGCCTGGATGTTCTTCAAAAACTTCTGCAAGTAAATACTTTGTGGATCTTATTATGTAA

Protein sequence

>MS.gene08783.t1

MSTRTLKTLFQFFSTFLFISHATATTLPSDIHDLLPDYGFPKGILPNNIASYTLSSSGYFTLHLQSPCYVRFSGQLVYYDTLVTGTLTYGSVSGVSGIQAKMLFIWLPVTGMEVDSRSGMLQFFVGALSKKLPANQFQDVPGCSSKTSASKYFVDLIM