Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09212.t1 | XP_013446572.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.30E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09212.t1 | I3SCJ8 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 2.4e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene09212 | 0.846947 | 1.58E-59 | -1.69E-46 |
| MS.gene050838 | MS.gene09212 | 0.819137 | 1.36E-52 | -1.69E-46 |
| MS.gene051063 | MS.gene09212 | 0.800986 | 1.11E-48 | -1.69E-46 |
| MS.gene051245 | MS.gene09212 | 0.835867 | 1.31E-56 | -1.69E-46 |
| MS.gene051746 | MS.gene09212 | 0.81802 | 2.44E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene09212 | 0.82531 | 5.03E-54 | -1.69E-46 |
| MS.gene051756 | MS.gene09212 | 0.867586 | 1.23E-65 | -1.69E-46 |
| MS.gene051903 | MS.gene09212 | 0.840262 | 9.67E-58 | -1.69E-46 |
| MS.gene053109 | MS.gene09212 | 0.807207 | 5.66E-50 | -1.69E-46 |
| MS.gene05380 | MS.gene09212 | 0.824251 | 8.93E-54 | -1.69E-46 |
| MS.gene053952 | MS.gene09212 | 0.818512 | 1.89E-52 | -1.69E-46 |
| MS.gene055788 | MS.gene09212 | 0.807134 | 5.86E-50 | -1.69E-46 |
| MS.gene056580 | MS.gene09212 | 0.802617 | 5.15E-49 | -1.69E-46 |
| MS.gene056601 | MS.gene09212 | 0.80173 | 7.84E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene09212 | 0.80938 | 1.95E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene09212 | 0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene09212 | 0.841866 | 3.66E-58 | -1.69E-46 |
| MS.gene057234 | MS.gene09212 | 0.84032 | 9.34E-58 | -1.69E-46 |
| MS.gene058055 | MS.gene09212 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene09212 | 0.817703 | 2.87E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene09212 | 0.841492 | 4.60E-58 | -1.69E-46 |
| MS.gene06001 | MS.gene09212 | 0.831152 | 1.96E-55 | -1.69E-46 |
| MS.gene061145 | MS.gene09212 | 0.830836 | 2.35E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09212.t1 | MTR_8g080630 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 3.22e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09212.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
| MS.gene09212.t1 | AT1G67350 | 73.810 | 84 | 22 | 0 | 1 | 84 | 1 | 84 | 1.33e-45 | 142 |
Find 29 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTACCCTATGCGACCTTA+TGG | 0.310546 | 4.1:+15140888 | MS.gene09212:CDS |
| AATCTACGATCCCTTTCATT+TGG | 0.347988 | 4.1:-15140810 | None:intergenic |
| CTATGCGACCTTATGGGTTC+TGG | 0.350279 | 4.1:+15140895 | MS.gene09212:CDS |
| AGTGAAAATGGCTTTCATAA+TGG | 0.354869 | 4.1:+15140749 | None:intergenic |
| ATGAAAGGGATCGTAGATTC+AGG | 0.366060 | 4.1:+15140814 | MS.gene09212:CDS |
| AATGGAATTCGCTGAGAATC+TGG | 0.378487 | 4.1:+15140767 | MS.gene09212:CDS |
| GTTACCCTATGCGACCTTAT+GGG | 0.431260 | 4.1:+15140889 | MS.gene09212:CDS |
| CCTCATTTCGACGACGAGGC+AGG | 0.443390 | 4.1:-15141014 | None:intergenic |
| GAATCTGGTTCTGAAATTGA+TGG | 0.457082 | 4.1:+15140782 | MS.gene09212:CDS |
| CCCAAGCAAGCTGAGAGTTA+TGG | 0.460911 | 4.1:-15140929 | None:intergenic |
| GCCATAACTCTCAGCTTGCT+TGG | 0.460971 | 4.1:+15140928 | MS.gene09212:CDS |
| CCATAACTCTCAGCTTGCTT+GGG | 0.463504 | 4.1:+15140929 | MS.gene09212:CDS |
| GCTCAGATTAGTCAAGTTGC+CGG | 0.484929 | 4.1:+15140954 | MS.gene09212:CDS |
| TGTGAAAGGATTTCATCATA+AGG | 0.496680 | 4.1:-15140987 | None:intergenic |
| CCTGCCTCGTCGTCGAAATG+AGG | 0.497822 | 4.1:+15141014 | MS.gene09212:CDS |
| CTCATTTCGACGACGAGGCA+GGG | 0.505092 | 4.1:-15141013 | None:intergenic |
| TCAAATGTCCAGAACCCATA+AGG | 0.513007 | 4.1:-15140903 | None:intergenic |
| TAGTCAAGTTGCCGGTCGTA+GGG | 0.521121 | 4.1:+15140962 | MS.gene09212:CDS |
| GATGGAAGATCCAAATGAAA+GGG | 0.524216 | 4.1:+15140800 | MS.gene09212:CDS |
| TGATGGAAGATCCAAATGAA+AGG | 0.535018 | 4.1:+15140799 | MS.gene09212:CDS |
| AGATAGATGCGCGAAGACGA+AGG | 0.541002 | 4.1:+15140857 | MS.gene09212:CDS |
| TGAAAGGGATCGTAGATTCA+GGG | 0.568833 | 4.1:+15140815 | MS.gene09212:CDS |
| CAGAACCCATAAGGTCGCAT+AGG | 0.571457 | 4.1:-15140894 | None:intergenic |
| GCGCGAAGACGAAGGAGATG+TGG | 0.571625 | 4.1:+15140865 | MS.gene09212:CDS |
| TTAGTCAAGTTGCCGGTCGT+AGG | 0.572671 | 4.1:+15140961 | MS.gene09212:CDS |
| ATCATAAGGATCCCTACGAC+CGG | 0.573881 | 4.1:-15140973 | None:intergenic |
| AGAACCCATAAGGTCGCATA+GGG | 0.582926 | 4.1:-15140893 | None:intergenic |
| ACGAGGCAGGGTAATGTGAA+AGG | 0.598363 | 4.1:-15141001 | None:intergenic |
| AAAACCTCATTTCGACGACG+AGG | 0.717022 | 4.1:-15141018 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTGAAAGGATTTCATCATA+AGG | - | chr4.1:15140990-15141009 | None:intergenic | 30.0% | |
| AATCTACGATCCCTTTCATT+TGG | - | chr4.1:15140813-15140832 | None:intergenic | 35.0% | |
| GATGGAAGATCCAAATGAAA+GGG | + | chr4.1:15140800-15140819 | MS.gene09212:CDS | 35.0% | |
| TGATGGAAGATCCAAATGAA+AGG | + | chr4.1:15140799-15140818 | MS.gene09212:CDS | 35.0% | |
| !! | GAATCTGGTTCTGAAATTGA+TGG | + | chr4.1:15140782-15140801 | MS.gene09212:CDS | 35.0% |
| AATGGAATTCGCTGAGAATC+TGG | + | chr4.1:15140767-15140786 | MS.gene09212:CDS | 40.0% | |
| ATGAAAGGGATCGTAGATTC+AGG | + | chr4.1:15140814-15140833 | MS.gene09212:CDS | 40.0% | |
| TCAAATGTCCAGAACCCATA+AGG | - | chr4.1:15140906-15140925 | None:intergenic | 40.0% | |
| TGAAAGGGATCGTAGATTCA+GGG | + | chr4.1:15140815-15140834 | MS.gene09212:CDS | 40.0% | |
| AGAACCCATAAGGTCGCATA+GGG | - | chr4.1:15140896-15140915 | None:intergenic | 45.0% | |
| AGTTACCCTATGCGACCTTA+TGG | + | chr4.1:15140888-15140907 | MS.gene09212:CDS | 45.0% | |
| ATCATAAGGATCCCTACGAC+CGG | - | chr4.1:15140976-15140995 | None:intergenic | 45.0% | |
| GTTACCCTATGCGACCTTAT+GGG | + | chr4.1:15140889-15140908 | MS.gene09212:CDS | 45.0% | |
| ! | CCATAACTCTCAGCTTGCTT+GGG | + | chr4.1:15140929-15140948 | MS.gene09212:CDS | 45.0% |
| ! | GCTCAGATTAGTCAAGTTGC+CGG | + | chr4.1:15140954-15140973 | MS.gene09212:CDS | 45.0% |
| ACGAGGCAGGGTAATGTGAA+AGG | - | chr4.1:15141004-15141023 | None:intergenic | 50.0% | |
| AGATAGATGCGCGAAGACGA+AGG | + | chr4.1:15140857-15140876 | MS.gene09212:CDS | 50.0% | |
| CAGAACCCATAAGGTCGCAT+AGG | - | chr4.1:15140897-15140916 | None:intergenic | 50.0% | |
| CCCAAGCAAGCTGAGAGTTA+TGG | - | chr4.1:15140932-15140951 | None:intergenic | 50.0% | |
| CTATGCGACCTTATGGGTTC+TGG | + | chr4.1:15140895-15140914 | MS.gene09212:CDS | 50.0% | |
| ! | GCCATAACTCTCAGCTTGCT+TGG | + | chr4.1:15140928-15140947 | MS.gene09212:CDS | 50.0% |
| ! | TAGTCAAGTTGCCGGTCGTA+GGG | + | chr4.1:15140962-15140981 | MS.gene09212:CDS | 50.0% |
| ! | TTAGTCAAGTTGCCGGTCGT+AGG | + | chr4.1:15140961-15140980 | MS.gene09212:CDS | 50.0% |
| GCGCGAAGACGAAGGAGATG+TGG | + | chr4.1:15140865-15140884 | MS.gene09212:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 15140756 | 15141034 | 15140756 | ID=MS.gene09212 |
| chr4.1 | mRNA | 15140756 | 15141034 | 15140756 | ID=MS.gene09212.t1;Parent=MS.gene09212 |
| chr4.1 | exon | 15140756 | 15141034 | 15140756 | ID=MS.gene09212.t1.exon1;Parent=MS.gene09212.t1 |
| chr4.1 | CDS | 15140756 | 15141034 | 15140756 | ID=cds.MS.gene09212.t1;Parent=MS.gene09212.t1 |
| Gene Sequence |
| Protein sequence |
