AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22666


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22666.t1 MTR_7g114920 92.913 127 9 0 60 186 1 127 5.31e-87 252
MS.gene22666.t1 MTR_1g100773 75.556 135 24 2 60 186 1 134 5.49e-70 209
MS.gene22666.t1 MTR_2g054650 53.125 128 45 5 62 185 4 120 7.84e-34 117
MS.gene22666.t1 MTR_4g053440 37.500 152 66 3 64 186 13 164 6.30e-30 108
MS.gene22666.t1 MTR_2g086190 43.056 144 66 5 56 186 4 144 6.97e-30 107
MS.gene22666.t1 MTR_1g060380 40.972 144 69 3 56 183 4 147 6.52e-29 105
MS.gene22666.t1 MTR_7g104320 38.776 147 61 3 64 183 15 159 2.32e-28 104
MS.gene22666.t1 MTR_0249s0070 33.537 164 82 4 47 183 5 168 9.23e-25 95.5
MS.gene22666.t1 MTR_0249s0070 34.839 155 74 4 56 183 6 160 1.10e-24 95.1
MS.gene22666.t1 MTR_2g098160 35.570 149 69 3 62 183 15 163 1.08e-23 92.4
MS.gene22666.t1 MTR_7g092400 35.333 150 68 4 62 183 103 251 9.06e-23 92.0
MS.gene22666.t1 MTR_3g028010 30.921 152 98 2 39 183 3 154 1.54e-22 89.4
MS.gene22666.t1 MTR_4g065570 34.646 127 61 3 79 183 2 128 1.10e-18 78.6
MS.gene22666.t1 MTR_8g036980 53.846 52 24 0 132 183 91 142 1.14e-16 73.6
MS.gene22666.t1 MTR_3g025570 50.000 46 23 0 125 170 2 47 4.43e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22666.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22666.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22666.t1 AT1G12440 42.361 144 61 4 62 183 16 159 2.78e-32 114
MS.gene22666.t1 AT4G12040 40.397 151 61 4 62 183 16 166 2.57e-30 109
MS.gene22666.t1 AT4G12040 40.397 151 61 4 62 183 16 166 2.57e-30 109
MS.gene22666.t1 AT4G22820 35.000 160 77 2 51 183 8 167 5.55e-29 106
MS.gene22666.t1 AT4G22820 35.000 160 77 2 51 183 8 167 5.55e-29 106
MS.gene22666.t1 AT4G14225 44.800 125 59 4 62 186 5 119 2.36e-26 97.8
MS.gene22666.t1 AT4G25380 42.857 119 61 2 65 183 10 121 1.38e-25 96.3
MS.gene22666.t1 AT3G52800 39.241 158 66 5 56 183 4 161 2.04e-25 97.1
MS.gene22666.t1 AT3G52800 39.241 158 66 5 56 183 4 161 2.04e-25 97.1
MS.gene22666.t1 AT2G36320 38.732 142 69 4 64 187 15 156 2.07e-25 96.7
MS.gene22666.t1 AT2G27580 34.000 150 66 3 62 183 10 154 5.61e-24 92.8
MS.gene22666.t1 AT2G27580 34.000 150 66 3 62 183 10 154 5.61e-24 92.8
MS.gene22666.t1 AT3G12630 36.364 121 70 3 70 183 31 151 1.52e-20 84.0
MS.gene22666.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22666.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22666.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4
MS.gene22666.t1 AT1G51200 34.000 150 71 3 62 183 15 164 8.00e-20 82.4

Find 46 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGCATGTATGTTCTTC+TGG 0.205127 7.2:+2401554 None:intergenic
GCCAATGGTTGTGGTTTCTA+TGG 0.283691 7.2:-2401836 MS.gene22666:CDS
CTTTGCATGTATGTTCTTCT+GGG 0.309729 7.2:+2401555 None:intergenic
AAGCCGATGACTCAAGAATA+AGG 0.356929 7.2:+2401756 None:intergenic
GAACAAAGGTTCTTGTTTGA+AGG 0.378685 7.2:+2401809 None:intergenic
GAAACAAAATCCTTTGTGTA+TGG 0.383600 7.2:-2401498 MS.gene22666:CDS
GACGGAGAAGGAGAGGGGTT+TGG 0.390926 7.2:-2402392 MS.gene22666:CDS
AAAGTGGATTTAAAAGAGAT+TGG 0.430845 7.2:-2401536 MS.gene22666:CDS
AGTGAGGGTTATGGCTTTGA+CGG 0.436346 7.2:-2402410 None:intergenic
TCACAAATATCATTGATACT+AGG 0.441086 7.2:+2401716 None:intergenic
GTGCGTAGTTTAAGAGAGGA+AGG 0.456139 7.2:-2402370 MS.gene22666:CDS
TCACTTTGTGCCAATGGTTG+TGG 0.466305 7.2:-2401845 MS.gene22666:CDS
AAACCTTATTCTTGAGTCAT+CGG 0.472750 7.2:-2401759 MS.gene22666:CDS
TTGATACTAGGGTTCTTTGA+AGG 0.481391 7.2:+2401728 None:intergenic
AACAAAATCCTTTGTGTATG+GGG 0.487990 7.2:-2401496 MS.gene22666:CDS
AAACAAAATCCTTTGTGTAT+GGG 0.488764 7.2:-2401497 MS.gene22666:CDS
CACAAATATCATTGATACTA+GGG 0.493722 7.2:+2401717 None:intergenic
GGTTATGGCTTTGACGGAGA+AGG 0.516948 7.2:-2402404 None:intergenic
GTTCCGTCACTTTGTGCCAA+TGG 0.523599 7.2:-2401851 MS.gene22666:CDS
TAAGAGAGGAAGGGGTGCGT+AGG 0.523850 7.2:-2402360 MS.gene22666:CDS
GGCTTTGACGGAGAAGGAGA+GGG 0.532470 7.2:-2402398 MS.gene22666:CDS
TGCGTAGTTTAAGAGAGGAA+GGG 0.537577 7.2:-2402369 MS.gene22666:CDS
CATTGTAACACTTTGAACAA+AGG 0.545148 7.2:+2401795 None:intergenic
AGGAGAAGAAAACTAGGTGC+AGG 0.548806 7.2:-2401652 MS.gene22666:CDS
TGGTGGTGATGAGAGGGATA+AGG 0.549250 7.2:-2402296 MS.gene22666:CDS
ACCATAGAAACCACAACCAT+TGG 0.550076 7.2:+2401835 None:intergenic
TTTGGTGCGTAGTTTAAGAG+AGG 0.557983 7.2:-2402374 MS.gene22666:CDS
AGAGTGATGGTGGTGATGAG+AGG 0.569060 7.2:-2402303 MS.gene22666:CDS
GCGTAGTTTAAGAGAGGAAG+GGG 0.569207 7.2:-2402368 MS.gene22666:CDS
GAGTGATGGTGGTGATGAGA+GGG 0.569582 7.2:-2402302 MS.gene22666:CDS
ACATGAAGGAGAAGAAAACT+AGG 0.590117 7.2:-2401658 MS.gene22666:CDS
TGGCTTTGACGGAGAAGGAG+AGG 0.597092 7.2:-2402399 MS.gene22666:CDS
AATGAGGAAGAGAGTGATGG+TGG 0.598981 7.2:-2402313 MS.gene22666:CDS
TGTGGAGGCATTGAAGAATG+AGG 0.609129 7.2:-2402329 MS.gene22666:CDS
CATTGACAATGATAACATGA+AGG 0.609470 7.2:-2401672 MS.gene22666:CDS
ACAGGGTTCAAGTGCCGTTG+TGG 0.610080 7.2:-2401605 MS.gene22666:CDS
CAACCATTGGCACAAAGTGA+CGG 0.611416 7.2:+2401848 None:intergenic
AGAAGAACATACATGCAAAG+TGG 0.612995 7.2:-2401552 MS.gene22666:CDS
GGTGCGTAGGTTAAGACGTG+TGG 0.614414 7.2:-2402347 MS.gene22666:CDS
ACTAAGTGAAACAACATGCA+TGG 0.623449 7.2:-2401873 MS.gene22666:CDS
AAGAATGAGGAAGAGAGTGA+TGG 0.624967 7.2:-2402316 MS.gene22666:CDS
ATTGAATTGAAAATCAACAA+TGG 0.628122 7.2:+2402270 None:intergenic
GCTTTGACGGAGAAGGAGAG+GGG 0.637010 7.2:-2402397 MS.gene22666:CDS
GCGTAGGTTAAGACGTGTGG+AGG 0.638442 7.2:-2402344 MS.gene22666:CDS
ACAAAATCCTTTGTGTATGG+GGG 0.652043 7.2:-2401495 MS.gene22666:CDS
CACAAAATACATCACCACAA+CGG 0.670499 7.2:+2401591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGGTAAAAAAAAAAA+GGG + chr7.2:2401956-2401975 None:intergenic 10.0%
!! TATATTTGGTAAAAAAAAAA+AGG + chr7.2:2401957-2401976 None:intergenic 10.0%
!!! TATAATTTTCAATTGAATTT+TGG - chr7.2:2401808-2401827 MS.gene22666:CDS 10.0%
!! GAAATAGTTGTAATTATATT+TGG + chr7.2:2401971-2401990 None:intergenic 15.0%
!! ATAAACAAAAATATAACCTG+CGG + chr7.2:2401762-2401781 None:intergenic 20.0%
!! ATTGAATTGAAAATCAACAA+TGG + chr7.2:2401617-2401636 None:intergenic 20.0%
!!! AAAAAGGGTATAGAATAATT+AGG + chr7.2:2401941-2401960 None:intergenic 20.0%
!!! GATTTTGTTTCTCTAATATT+TGG + chr7.2:2402377-2402396 None:intergenic 20.0%
! AAACAAAATCCTTTGTGTAT+GGG - chr7.2:2402387-2402406 MS.gene22666:CDS 25.0%
! CACAAATATCATTGATACTA+GGG + chr7.2:2402170-2402189 None:intergenic 25.0%
! TCACAAATATCATTGATACT+AGG + chr7.2:2402171-2402190 None:intergenic 25.0%
!! AAAGTGGATTTAAAAGAGAT+TGG - chr7.2:2402348-2402367 MS.gene22666:CDS 25.0%
AAACCTTATTCTTGAGTCAT+CGG - chr7.2:2402125-2402144 MS.gene22666:intron 30.0%
AACAAAATCCTTTGTGTATG+GGG - chr7.2:2402388-2402407 MS.gene22666:CDS 30.0%
ACAAAAGAGATGAGATAGAT+AGG + chr7.2:2401688-2401707 None:intergenic 30.0%
AGCATATATCGTTTAGAAGT+TGG + chr7.2:2401886-2401905 None:intergenic 30.0%
CATTGACAATGATAACATGA+AGG - chr7.2:2402212-2402231 MS.gene22666:intron 30.0%
CATTGTAACACTTTGAACAA+AGG + chr7.2:2402092-2402111 None:intergenic 30.0%
GAAACAAAATCCTTTGTGTA+TGG - chr7.2:2402386-2402405 MS.gene22666:CDS 30.0%
GATTCATTCACATTCATATG+CGG - chr7.2:2401906-2401925 MS.gene22666:intron 30.0%
! GATTATAGAACTGAAGTGTT+GGG + chr7.2:2401666-2401685 None:intergenic 30.0%
AACAAAAAAGTGGGACTAAC+AGG - chr7.2:2402261-2402280 MS.gene22666:intron 35.0%
ACAAAAAAGTGGGACTAACA+GGG - chr7.2:2402262-2402281 MS.gene22666:intron 35.0%
ACAAAATCCTTTGTGTATGG+GGG - chr7.2:2402389-2402408 MS.gene22666:CDS 35.0%
ACAATCCAACTTTGCTGAAA+AGG + chr7.2:2401840-2401859 None:intergenic 35.0%
ACATGAAGGAGAAGAAAACT+AGG - chr7.2:2402226-2402245 MS.gene22666:intron 35.0%
ACTAAGTGAAACAACATGCA+TGG - chr7.2:2402011-2402030 MS.gene22666:intron 35.0%
ACTTTGCATGTATGTTCTTC+TGG + chr7.2:2402333-2402352 None:intergenic 35.0%
AGAAGAACATACATGCAAAG+TGG - chr7.2:2402332-2402351 MS.gene22666:CDS 35.0%
AGGAGTTGCAACAAAAAAGT+GGG - chr7.2:2402252-2402271 MS.gene22666:intron 35.0%
CACAAAATACATCACCACAA+CGG + chr7.2:2402296-2402315 None:intergenic 35.0%
CTTTGCATGTATGTTCTTCT+GGG + chr7.2:2402332-2402351 None:intergenic 35.0%
GGATTATAGAACTGAAGTGT+TGG + chr7.2:2401667-2401686 None:intergenic 35.0%
TCAGCAAAGTTGGATTGTAT+TGG - chr7.2:2401842-2401861 MS.gene22666:CDS 35.0%
TTGATACTAGGGTTCTTTGA+AGG + chr7.2:2402159-2402178 None:intergenic 35.0%
! ATCGTTTAGAAGTTGGTTGT+TGG + chr7.2:2401879-2401898 None:intergenic 35.0%
! GAACAAAGGTTCTTGTTTGA+AGG + chr7.2:2402078-2402097 None:intergenic 35.0%
! GTTTTCCTTTTCAGCAAAGT+TGG - chr7.2:2401832-2401851 MS.gene22666:CDS 35.0%
!! CTCATCTCTTTTGTTGTGTT+TGG - chr7.2:2401694-2401713 MS.gene22666:CDS 35.0%
!! TCTCTTTTGTTGTGTTTGGT+TGG - chr7.2:2401698-2401717 MS.gene22666:CDS 35.0%
AAGAATGAGGAAGAGAGTGA+TGG - chr7.2:2401568-2401587 MS.gene22666:CDS 40.0%
AAGCCGATGACTCAAGAATA+AGG + chr7.2:2402131-2402150 None:intergenic 40.0%
ACCATAGAAACCACAACCAT+TGG + chr7.2:2402052-2402071 None:intergenic 40.0%
CAAAAATATAACCTGCGGTG+TGG + chr7.2:2401757-2401776 None:intergenic 40.0%
CAGGAGTTGCAACAAAAAAG+TGG - chr7.2:2402251-2402270 MS.gene22666:intron 40.0%
TGCGTAGTTTAAGAGAGGAA+GGG - chr7.2:2401515-2401534 MS.gene22666:CDS 40.0%
! TTTGGTGCGTAGTTTAAGAG+AGG - chr7.2:2401510-2401529 MS.gene22666:CDS 40.0%
!! CGTTGTGGTGATGTATTTTG+TGG - chr7.2:2402294-2402313 MS.gene22666:CDS 40.0%
AATGAGGAAGAGAGTGATGG+TGG - chr7.2:2401571-2401590 MS.gene22666:CDS 45.0%
AGGAGAAGAAAACTAGGTGC+AGG - chr7.2:2402232-2402251 MS.gene22666:intron 45.0%
ATATAACCTGCGGTGTGGTT+TGG + chr7.2:2401752-2401771 None:intergenic 45.0%
CAACCATTGGCACAAAGTGA+CGG + chr7.2:2402039-2402058 None:intergenic 45.0%
CTTATCACCCCCATACACAA+AGG + chr7.2:2402399-2402418 None:intergenic 45.0%
GCCAATGGTTGTGGTTTCTA+TGG - chr7.2:2402048-2402067 MS.gene22666:intron 45.0%
GCGTAGTTTAAGAGAGGAAG+GGG - chr7.2:2401516-2401535 MS.gene22666:CDS 45.0%
GTGCGTAGTTTAAGAGAGGA+AGG - chr7.2:2401514-2401533 MS.gene22666:CDS 45.0%
TCACTTTGTGCCAATGGTTG+TGG - chr7.2:2402039-2402058 MS.gene22666:intron 45.0%
!! TGTGGAGGCATTGAAGAATG+AGG - chr7.2:2401555-2401574 MS.gene22666:CDS 45.0%
! GTTCCGTCACTTTGTGCCAA+TGG - chr7.2:2402033-2402052 MS.gene22666:intron 50.0%
! TGGTGGTGATGAGAGGGATA+AGG - chr7.2:2401588-2401607 MS.gene22666:CDS 50.0%
!! AGAGTGATGGTGGTGATGAG+AGG - chr7.2:2401581-2401600 MS.gene22666:CDS 50.0%
!! GAGTGATGGTGGTGATGAGA+GGG - chr7.2:2401582-2401601 MS.gene22666:CDS 50.0%
ACCAAACCAAACCACACCGC+AGG - chr7.2:2401743-2401762 MS.gene22666:CDS 55.0%
GCGTAGGTTAAGACGTGTGG+AGG - chr7.2:2401540-2401559 MS.gene22666:CDS 55.0%
GCTTTGACGGAGAAGGAGAG+GGG - chr7.2:2401487-2401506 MS.gene22666:CDS 55.0%
GGTGCGTAGGTTAAGACGTG+TGG - chr7.2:2401537-2401556 MS.gene22666:CDS 55.0%
! ACAGGGTTCAAGTGCCGTTG+TGG - chr7.2:2402279-2402298 MS.gene22666:CDS 55.0%
! GGCTTTGACGGAGAAGGAGA+GGG - chr7.2:2401486-2401505 MS.gene22666:CDS 55.0%
! TAAGAGAGGAAGGGGTGCGT+AGG - chr7.2:2401524-2401543 MS.gene22666:CDS 55.0%
!! ACCTGCGGTGTGGTTTGGTT+TGG + chr7.2:2401747-2401766 None:intergenic 55.0%
!! TGGCTTTGACGGAGAAGGAG+AGG - chr7.2:2401485-2401504 MS.gene22666:CDS 55.0%
GACGGAGAAGGAGAGGGGTT+TGG - chr7.2:2401492-2401511 MS.gene22666:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 2401484 2402422 2401484 ID=MS.gene22666
chr7.2 mRNA 2401484 2402422 2401484 ID=MS.gene22666.t1;Parent=MS.gene22666
chr7.2 exon 2402270 2402422 2402270 ID=MS.gene22666.t1.exon1;Parent=MS.gene22666.t1
chr7.2 CDS 2402270 2402422 2402270 ID=cds.MS.gene22666.t1;Parent=MS.gene22666.t1
chr7.2 exon 2401484 2401900 2401484 ID=MS.gene22666.t1.exon2;Parent=MS.gene22666.t1
chr7.2 CDS 2401484 2401900 2401484 ID=cds.MS.gene22666.t1;Parent=MS.gene22666.t1
Gene Sequence

>MS.gene22666

ATGGCTTTGACGGAGAAGGAGAGGGGTTTGGTGCGTAGTTTAAGAGAGGAAGGGGTGCGTAGGTTAAGACGTGTGGAGGCATTGAAGAATGAGGAAGAGAGTGATGGTGGTGATGAGAGGGATAAGGATGCCATTGTTGATTTTCAATTCAATGTAATGTAAAAGTGATTCAAACTATACCCAACACTTCAGTTCTATAATCCTATCTATCTCATCTCTTTTGTTGTGTTTGGTTGGTGTTTAGATTTAGATTAGATTGACCAAACCAAACCACACCGCAGGTTATATTTTTGTTTATTTTAGTATATTGCTTTTATCTTTTACTATAATTTTCAATTGAATTTTGGCGTTTTCCTTTTCAGCAAAGTTGGATTGTATTGGATGTTTCTTTTCCAACAACCAACTTCTAAACGATATATGCTGATTCATTCACATTCATATGCGGATTCATTCACCTAATTATTCTATACCCTTTTTTTTTTTACCAAATATAATTACAACTATTTCAATCTTTTTCTTTAGTCAAAACTAAGTGAAACAACATGCATGGTTCCGTCACTTTGTGCCAATGGTTGTGGTTTCTATGGTTCTCCTTCAAACAAGAACCTTTGTTCAAAGTGTTACAATGTTTATCTCAAAGAAAACCTTATTCTTGAGTCATCGGCTTCTTGTCCTTCAAAGAACCCTAGTATCAATGATATTTGTGATGCTGTGACTGCTATTTCTCTCATTGACAATGATAACATGAAGGAGAAGAAAACTAGGTGCAGGAGTTGCAACAAAAAAGTGGGACTAACAGGGTTCAAGTGCCGTTGTGGTGATGTATTTTGTGGAATGCATAGATACCCAGAAGAACATACATGCAAAGTGGATTTAAAAGAGATTGGTCGCCAAATATTAGAGAAACAAAATCCTTTGTGTATGGGGGTGATAAGTTAG

Protein sequence

>MS.gene22666.t1

MALTEKERGLVRSLREEGVRRLRRVEALKNEEESDGGDERDKDAIVDFQFNSKLSETTCMVPSLCANGCGFYGSPSNKNLCSKCYNVYLKENLILESSASCPSKNPSINDICDAVTAISLIDNDNMKEKKTRCRSCNKKVGLTGFKCRCGDVFCGMHRYPEEHTCKVDLKEIGRQILEKQNPLCMGVIS