Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23097.t1 | XP_003626077.1 | 97.4 | 195 | 5 | 0 | 1 | 195 | 83 | 277 | 5.10E-114 | 420.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23097.t1 | Q5NB11 | 74.4 | 195 | 50 | 0 | 1 | 195 | 96 | 290 | 1.5e-90 | 333.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23097.t1 | G7KUN3 | 97.4 | 195 | 5 | 0 | 1 | 195 | 83 | 277 | 3.7e-114 | 420.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene23097 | -0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene23097 | -0.810026 | 1.41E-50 | -1.69E-46 |
| MS.gene049402 | MS.gene23097 | 0.819137 | 1.36E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene23097 | -0.818919 | 1.52E-52 | -1.69E-46 |
| MS.gene050165 | MS.gene23097 | -0.835432 | 1.68E-56 | -1.69E-46 |
| MS.gene050333 | MS.gene23097 | -0.814113 | 1.82E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene23097 | -0.825437 | 4.69E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene23097 | -0.823785 | 1.15E-53 | -1.69E-46 |
| MS.gene050832 | MS.gene23097 | 0.997813 | 9.70E-250 | -1.69E-46 |
| MS.gene050833 | MS.gene23097 | 0.967389 | 3.29E-127 | -1.69E-46 |
| MS.gene050883 | MS.gene23097 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene051364 | MS.gene23097 | 0.800933 | 1.14E-48 | -1.69E-46 |
| MS.gene054642 | MS.gene23097 | -0.800297 | 1.54E-48 | -1.69E-46 |
| MS.gene054954 | MS.gene23097 | -0.800226 | 1.59E-48 | -1.69E-46 |
| MS.gene055502 | MS.gene23097 | -0.809007 | 2.34E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene23097 | -0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene23097 | -0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene23097 | -0.821405 | 4.11E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene23097 | 0.802279 | 6.05E-49 | -1.69E-46 |
| MS.gene058111 | MS.gene23097 | 0.826879 | 2.13E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene23097 | -0.821059 | 4.94E-53 | -1.69E-46 |
| MS.gene059479 | MS.gene23097 | 0.813512 | 2.47E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene23097 | -0.819051 | 1.42E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene23097 | -0.805176 | 1.52E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene23097 | -0.800601 | 1.34E-48 | -1.69E-46 |
| MS.gene060823 | MS.gene23097 | 0.82103 | 5.01E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGATACTGGACCTAATT+TGG | 0.146711 | 7.4:-5179680 | MS.gene23097:CDS |
| TGTGATGGTTGGAAAATATT+TGG | 0.256663 | 7.4:-5179851 | MS.gene23097:CDS |
| TCAAAACTGCTCTATGGTTT+TGG | 0.260706 | 7.4:-5179909 | MS.gene23097:CDS |
| GCTAATCGCACAGCTGGTTT+TGG | 0.280719 | 7.4:-5179808 | MS.gene23097:CDS |
| TGATATGCACAATCCAAATT+AGG | 0.305058 | 7.4:+5179667 | None:intergenic |
| TTGTGTAGGAATTATAACTA+TGG | 0.344973 | 7.4:-5180006 | MS.gene23097:intron |
| AAAGATTGGCCTTGCTTTGA+AGG | 0.347412 | 7.4:-5180712 | MS.gene23097:CDS |
| TTGCTTTCAAAACTGCTCTA+TGG | 0.359827 | 7.4:-5179915 | MS.gene23097:CDS |
| AAGCATAAGCCCCAAGAAAA+TGG | 0.365932 | 7.4:+5180781 | None:intergenic |
| GCAAGAGTTAATGATCGAAT+TGG | 0.385700 | 7.4:-5179733 | MS.gene23097:CDS |
| AAGTTGTTCAATGTGGATAC+TGG | 0.403395 | 7.4:-5179691 | MS.gene23097:CDS |
| GAATTGTTTGATACAATCTC+TGG | 0.409450 | 7.4:+5179943 | None:intergenic |
| GGGAAGGCGTTAGGATTTGA+TGG | 0.420884 | 7.4:-5179976 | MS.gene23097:CDS |
| CCCCAAGAAAATGGTCCATC+AGG | 0.433362 | 7.4:+5180790 | None:intergenic |
| GTATACCAAGATAGTTGAAT+AGG | 0.440524 | 7.4:+5180667 | None:intergenic |
| TCATTAACTCTTGCATCATC+AGG | 0.440723 | 7.4:+5179742 | None:intergenic |
| GAGGTCCTATTCAACTATCT+TGG | 0.447585 | 7.4:-5180672 | MS.gene23097:intron |
| GGGTGGGCCACTGCACCTGA+TGG | 0.450425 | 7.4:-5180805 | MS.gene23097:CDS |
| GAATCACAGTAGTTACTTTG+AGG | 0.463755 | 7.4:+5180742 | None:intergenic |
| GGACCAGCAGGGAAGGCGTT+AGG | 0.472444 | 7.4:-5179985 | MS.gene23097:CDS |
| AAATATAGTCAATGGTGGAT+TGG | 0.476185 | 7.4:-5179776 | MS.gene23097:CDS |
| AATTATAACTATGGACCAGC+AGG | 0.477533 | 7.4:-5179997 | MS.gene23097:CDS |
| AATCCTAACGCCTTCCCTGC+TGG | 0.489708 | 7.4:+5179982 | None:intergenic |
| ACATTGTGACAAGAAGGTAT+TGG | 0.494924 | 7.4:+5179871 | None:intergenic |
| GTGATTCTACTGACAAAGAT+TGG | 0.496296 | 7.4:-5180726 | MS.gene23097:CDS |
| CAGATTTCACATGAAACTAC+TGG | 0.531637 | 7.4:-5180829 | MS.gene23097:CDS |
| ATAGAAGCTAATCGCACAGC+TGG | 0.541134 | 7.4:-5179814 | MS.gene23097:CDS |
| AGCTATCTCACGCTTTCTTG+TGG | 0.548914 | 7.4:+5180864 | None:intergenic |
| GGCAAAAGTTACAAAGGAAG+AGG | 0.557753 | 7.4:-5180691 | MS.gene23097:CDS |
| ACCATCACATTGTGACAAGA+AGG | 0.564779 | 7.4:+5179865 | None:intergenic |
| ACATGAAACTACTGGTGGGT+GGG | 0.569584 | 7.4:-5180821 | MS.gene23097:CDS |
| GGAAGGCGTTAGGATTTGAT+GGG | 0.584389 | 7.4:-5179975 | MS.gene23097:CDS |
| GTCAATGGTGGATTGGAATG+TGG | 0.588138 | 7.4:-5179769 | MS.gene23097:CDS |
| TTTGAAGGCAAAAGTTACAA+AGG | 0.591242 | 7.4:-5180697 | MS.gene23097:CDS |
| TCTTGTCACAATGTGATGGT+TGG | 0.593829 | 7.4:-5179862 | MS.gene23097:CDS |
| CACATGAAACTACTGGTGGG+TGG | 0.597128 | 7.4:-5180822 | MS.gene23097:CDS |
| TAACTATGGACCAGCAGGGA+AGG | 0.603344 | 7.4:-5179992 | MS.gene23097:CDS |
| AAATGGTCCATCAGGTGCAG+TGG | 0.617726 | 7.4:+5180798 | None:intergenic |
| GTGACAAATATAGTCAATGG+TGG | 0.618848 | 7.4:-5179781 | MS.gene23097:CDS |
| TTGGTGACAAATATAGTCAA+TGG | 0.622222 | 7.4:-5179784 | MS.gene23097:CDS |
| ACCTTCTTGTCACAATGTGA+TGG | 0.640940 | 7.4:-5179866 | MS.gene23097:CDS |
| ATTTCACATGAAACTACTGG+TGG | 0.650794 | 7.4:-5180826 | MS.gene23097:CDS |
| ATTATAACTATGGACCAGCA+GGG | 0.676909 | 7.4:-5179996 | MS.gene23097:CDS |
| ATATACTAAGTTGTTCAATG+TGG | 0.678623 | 7.4:-5179698 | MS.gene23097:CDS |
| TTTCACATGAAACTACTGGT+GGG | 0.698219 | 7.4:-5180825 | MS.gene23097:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTAGATTAAATTATTATT+TGG | - | chr7.4:5179907-5179926 | MS.gene23097:CDS | 10.0% |
| !! | ATACGAAATAATTACTAAAT+CGG | + | chr7.4:5180114-5180133 | None:intergenic | 15.0% |
| !! | TGTCAACAAAAAAAAAAAAA+AGG | - | chr7.4:5180286-5180305 | MS.gene23097:intron | 15.0% |
| !! | TACGAAATAATTACTAAATC+GGG | + | chr7.4:5180113-5180132 | None:intergenic | 20.0% |
| !! | TGATTAAGTATAATATGGTT+GGG | + | chr7.4:5180347-5180366 | None:intergenic | 20.0% |
| !! | TTGATTAAGTATAATATGGT+TGG | + | chr7.4:5180348-5180367 | None:intergenic | 20.0% |
| ! | ATATACTAAGTTGTTCAATG+TGG | - | chr7.4:5180829-5180848 | MS.gene23097:CDS | 25.0% |
| ! | TCGCTTGATTAAGTATAATA+TGG | + | chr7.4:5180352-5180371 | None:intergenic | 25.0% |
| ! | TTGTGTAGGAATTATAACTA+TGG | - | chr7.4:5180521-5180540 | MS.gene23097:intron | 25.0% |
| !!! | CATAGAAACAATTTTAGGTT+TGG | + | chr7.4:5179966-5179985 | None:intergenic | 25.0% |
| AGCTAATGAGTTTCAGTTAA+AGG | - | chr7.4:5180384-5180403 | MS.gene23097:intron | 30.0% | |
| CTACACAAAAGTTTATGAGA+GGG | + | chr7.4:5180509-5180528 | None:intergenic | 30.0% | |
| GAATTGTTTGATACAATCTC+TGG | + | chr7.4:5180587-5180606 | None:intergenic | 30.0% | |
| GTATACCAAGATAGTTGAAT+AGG | + | chr7.4:5179863-5179882 | None:intergenic | 30.0% | |
| TACACAAAAGTTTATGAGAG+GGG | + | chr7.4:5180508-5180527 | None:intergenic | 30.0% | |
| TGATATGCACAATCCAAATT+AGG | + | chr7.4:5180863-5180882 | None:intergenic | 30.0% | |
| TGTGATGGTTGGAAAATATT+TGG | - | chr7.4:5180676-5180695 | MS.gene23097:CDS | 30.0% | |
| TTTGAAGGCAAAAGTTACAA+AGG | - | chr7.4:5179830-5179849 | MS.gene23097:CDS | 30.0% | |
| ! | AGGATCATATGGATTAAAAC+TGG | + | chr7.4:5180044-5180063 | None:intergenic | 30.0% |
| ! | GAGCTCATAGAAACAATTTT+AGG | + | chr7.4:5179971-5179990 | None:intergenic | 30.0% |
| ! | TTGGTGACAAATATAGTCAA+TGG | - | chr7.4:5180743-5180762 | MS.gene23097:CDS | 30.0% |
| ! | TTTGAAACTAATTTAGCGAC+CGG | + | chr7.4:5180157-5180176 | None:intergenic | 30.0% |
| !! | AAATATAGTCAATGGTGGAT+TGG | - | chr7.4:5180751-5180770 | MS.gene23097:CDS | 30.0% |
| AAAACTTATTTCCCAAGCTG+AGG | + | chr7.4:5180069-5180088 | None:intergenic | 35.0% | |
| AAGTTGTTCAATGTGGATAC+TGG | - | chr7.4:5180836-5180855 | MS.gene23097:CDS | 35.0% | |
| AATTATAACTATGGACCAGC+AGG | - | chr7.4:5180530-5180549 | MS.gene23097:intron | 35.0% | |
| AGCAGCGTTAAAATTCTTGA+CGG | - | chr7.4:5180018-5180037 | MS.gene23097:intron | 35.0% | |
| ATTATAACTATGGACCAGCA+GGG | - | chr7.4:5180531-5180550 | MS.gene23097:intron | 35.0% | |
| ATTTCACATGAAACTACTGG+TGG | - | chr7.4:5179701-5179720 | MS.gene23097:CDS | 35.0% | |
| CAGATTTCACATGAAACTAC+TGG | - | chr7.4:5179698-5179717 | MS.gene23097:CDS | 35.0% | |
| CCTACACAAAAGTTTATGAG+AGG | + | chr7.4:5180510-5180529 | None:intergenic | 35.0% | |
| GCAAGAGTTAATGATCGAAT+TGG | - | chr7.4:5180794-5180813 | MS.gene23097:CDS | 35.0% | |
| GTGACAAATATAGTCAATGG+TGG | - | chr7.4:5180746-5180765 | MS.gene23097:CDS | 35.0% | |
| GTGATTCTACTGACAAAGAT+TGG | - | chr7.4:5179801-5179820 | MS.gene23097:CDS | 35.0% | |
| TATTTCGTATCTGTAGTGAC+CGG | - | chr7.4:5180124-5180143 | MS.gene23097:intron | 35.0% | |
| TCATTAACTCTTGCATCATC+AGG | + | chr7.4:5180788-5180807 | None:intergenic | 35.0% | |
| TGTGGATTTGGACAAAGAAT+TGG | + | chr7.4:5179940-5179959 | None:intergenic | 35.0% | |
| TTGCTTTCAAAACTGCTCTA+TGG | - | chr7.4:5180612-5180631 | MS.gene23097:intron | 35.0% | |
| TTTCACATGAAACTACTGGT+GGG | - | chr7.4:5179702-5179721 | MS.gene23097:CDS | 35.0% | |
| ! | ACATTGTGACAAGAAGGTAT+TGG | + | chr7.4:5180659-5180678 | None:intergenic | 35.0% |
| ! | CCTCTCATAAACTTTTGTGT+AGG | - | chr7.4:5180507-5180526 | MS.gene23097:intron | 35.0% |
| ! | GAATCACAGTAGTTACTTTG+AGG | + | chr7.4:5179788-5179807 | None:intergenic | 35.0% |
| ! | TAACTTTTGCCTTCAAAGCA+AGG | + | chr7.4:5179827-5179846 | None:intergenic | 35.0% |
| ! | TCAAAACTGCTCTATGGTTT+TGG | - | chr7.4:5180618-5180637 | MS.gene23097:intron | 35.0% |
| !!! | CAATTTTAGGTTTGGATGTG+TGG | + | chr7.4:5179958-5179977 | None:intergenic | 35.0% |
| AAGCATAAGCCCCAAGAAAA+TGG | + | chr7.4:5179749-5179768 | None:intergenic | 40.0% | |
| ACCATCACATTGTGACAAGA+AGG | + | chr7.4:5180665-5180684 | None:intergenic | 40.0% | |
| ACCTTCTTGTCACAATGTGA+TGG | - | chr7.4:5180661-5180680 | MS.gene23097:intron | 40.0% | |
| GAGGTCCTATTCAACTATCT+TGG | - | chr7.4:5179855-5179874 | MS.gene23097:CDS | 40.0% | |
| GGCAAAAGTTACAAAGGAAG+AGG | - | chr7.4:5179836-5179855 | MS.gene23097:CDS | 40.0% | |
| TAGGTTTGGATGTGTGGATT+TGG | + | chr7.4:5179952-5179971 | None:intergenic | 40.0% | |
| TCTTGTCACAATGTGATGGT+TGG | - | chr7.4:5180665-5180684 | MS.gene23097:intron | 40.0% | |
| TGTGGATACTGGACCTAATT+TGG | - | chr7.4:5180847-5180866 | MS.gene23097:CDS | 40.0% | |
| TTTAGCGACCGGTTATAAAC+CGG | + | chr7.4:5180146-5180165 | None:intergenic | 40.0% | |
| ! | TGTAGTGACCGGTTTATAAC+CGG | - | chr7.4:5180135-5180154 | MS.gene23097:intron | 40.0% |
| !! | AAAGATTGGCCTTGCTTTGA+AGG | - | chr7.4:5179815-5179834 | MS.gene23097:CDS | 40.0% |
| !! | ACCTGATGGACCATTTTCTT+GGG | - | chr7.4:5179736-5179755 | MS.gene23097:CDS | 40.0% |
| AAGCTGAGGTGAGGATCATA+TGG | + | chr7.4:5180055-5180074 | None:intergenic | 45.0% | |
| AGCTATCTCACGCTTTCTTG+TGG | + | chr7.4:5179666-5179685 | None:intergenic | 45.0% | |
| ATAGAAGCTAATCGCACAGC+TGG | - | chr7.4:5180713-5180732 | MS.gene23097:CDS | 45.0% | |
| ATATGATCCTCACCTCAGCT+TGG | - | chr7.4:5180054-5180073 | MS.gene23097:intron | 45.0% | |
| TATGATCCTCACCTCAGCTT+GGG | - | chr7.4:5180055-5180074 | MS.gene23097:intron | 45.0% | |
| TTATTTCCCAAGCTGAGGTG+AGG | + | chr7.4:5180064-5180083 | None:intergenic | 45.0% | |
| ! | ACATGAAACTACTGGTGGGT+GGG | - | chr7.4:5179706-5179725 | MS.gene23097:CDS | 45.0% |
| !! | CACCTGATGGACCATTTTCT+TGG | - | chr7.4:5179735-5179754 | MS.gene23097:CDS | 45.0% |
| !! | CCTGATGGACCATTTTCTTG+GGG | - | chr7.4:5179737-5179756 | MS.gene23097:CDS | 45.0% |
| !! | GGAAGGCGTTAGGATTTGAT+GGG | - | chr7.4:5180552-5180571 | MS.gene23097:intron | 45.0% |
| !! | GTCAATGGTGGATTGGAATG+TGG | - | chr7.4:5180758-5180777 | MS.gene23097:CDS | 45.0% |
| AAATGGTCCATCAGGTGCAG+TGG | + | chr7.4:5179732-5179751 | None:intergenic | 50.0% | |
| CACATGAAACTACTGGTGGG+TGG | - | chr7.4:5179705-5179724 | MS.gene23097:CDS | 50.0% | |
| CCCCAAGAAAATGGTCCATC+AGG | + | chr7.4:5179740-5179759 | None:intergenic | 50.0% | |
| TAACTATGGACCAGCAGGGA+AGG | - | chr7.4:5180535-5180554 | MS.gene23097:intron | 50.0% | |
| !! | GCTAATCGCACAGCTGGTTT+TGG | - | chr7.4:5180719-5180738 | MS.gene23097:CDS | 50.0% |
| !! | GGGAAGGCGTTAGGATTTGA+TGG | - | chr7.4:5180551-5180570 | MS.gene23097:intron | 50.0% |
| !!! | TCGCACAGCTGGTTTTGGAT+TGG | - | chr7.4:5180724-5180743 | MS.gene23097:CDS | 50.0% |
| AATCCTAACGCCTTCCCTGC+TGG | + | chr7.4:5180548-5180567 | None:intergenic | 55.0% | |
| !! | GGACCAGCAGGGAAGGCGTT+AGG | - | chr7.4:5180542-5180561 | MS.gene23097:intron | 65.0% |
| GGGTGGGCCACTGCACCTGA+TGG | - | chr7.4:5179722-5179741 | MS.gene23097:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 5179654 | 5180895 | 5179654 | ID=MS.gene23097 |
| chr7.4 | mRNA | 5179654 | 5180895 | 5179654 | ID=MS.gene23097.t1;Parent=MS.gene23097 |
| chr7.4 | exon | 5180673 | 5180895 | 5180673 | ID=MS.gene23097.t1.exon1;Parent=MS.gene23097.t1 |
| chr7.4 | CDS | 5180673 | 5180895 | 5180673 | ID=cds.MS.gene23097.t1;Parent=MS.gene23097.t1 |
| chr7.4 | exon | 5179654 | 5180020 | 5179654 | ID=MS.gene23097.t1.exon2;Parent=MS.gene23097.t1 |
| chr7.4 | CDS | 5179654 | 5180020 | 5179654 | ID=cds.MS.gene23097.t1;Parent=MS.gene23097.t1 |
| Gene Sequence |
| Protein sequence |
